Rw6G027230

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001162
Physical Location & Seq
Forward (+)
50963107 .. 50967694
4588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027230.1

Sequence Viewer

Length: 702 bp
ATGGATCATGCTGGCATCAGAACAGAGGAGTGGTATGGAAGGAATTCAAGGGGTATGGAAATTTTCTGCAAAAGCTGGTTGCCAAAACCAGGTGATCAAATCAAAGCTGGCTTATGTTTCTGCCATGGGTATGGTAGTACTTGCACATTCTTCTTTGAAGGTATTGCTAGGAGAATTGCTCAGTCTGGGTACGCTGTGTATGCAGTAGACTATCCAGGTTTTGGTCTTTCTGAAGGGTTGCATGGTTTCATCCCAAACTTTGATGAGTTGGTCGATGATGTTATTGAACAATTTACTAGTATCAAAGGTGTTTGCCCTTTTTTTATAATGGGGGAGTCGATGGGTGGAGCTGTCACTGTAAAGATTCATCTAAAGGAACCGCATGAATGGGATGGAGTGATCCTTGTAGCTCCAATGTTGGCCTTTACTTGGTGTACTGTTTGCGAAATTTTCCCATCGAAAAAAGATAATAATTATCAAGACAAAAAAAAAAAAAAAGGGGTTGCAAGATATTCGGAGGCTTCCCCTATTCAGCTTGTGGAACTCCTGGAAGCTGGTAATGGAGCGAAAGGAAAATCTCTTTTTGATTTCTCAATAGCTACTGTTACCATCCAGCTAGTCACAACAAAATTGTTCCTGGGTCACACCTTCGATCGATTTTGGGCCTGGTCTTCGCTCTATCAAGAGGGCTATCGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

233

Amino Acids

26.16

Weight (kDa)

6.31

Isoelectric Point (pI)

28.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 33 - 143 1.4e-30 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 38 - 164 8.8e-12 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 40 - 142 4.1e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 221
AccI GTMKAC 1 cut(s) 207
AciI CCGC 1 cut(s) 380
AclWI GGATC 2 cut(s) 12, 394
AcsI RAATTY 3 cut(s) 43, 60, 447
AcuI CTGAAG 1 cut(s) 252
AfaI GTAC 3 cut(s) 139, 191, 436
AfiI CCNNNNNNNGG 3 cut(s) 89, 221, 429
AgsI TTSAA 3 cut(s) 48, 158, 287
AhlI ACTAGT 1 cut(s) 296
AjnI CCWGG 5 cut(s) 88, 214, 546, 636, 665
AluBI AGCT 8 cut(s) 75, 107, 350, 410, 535, 554, 599, 616
AluI AGCT 8 cut(s) 75, 107, 350, 410, 535, 554, 599, 616
AlwI GGATC 2 cut(s) 12, 394
AoxI GGCC 2 cut(s) 420, 663
ApeKI GCWGC 1 cut(s) 696
ApoI RAATTY 3 cut(s) 43, 60, 447
Asp700I GAANNNNTTC 1 cut(s) 43
AspS9I GGNCC 1 cut(s) 663
AsuHPI GGTGA 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 663
BbvI GCAGC 1 cut(s) 683
BccI CCATC 4 cut(s) 334, 386, 463, 617
BcgI CGANNNNNNTGC 2 cut(s) 495, 529
BciT130I CCWGG 5 cut(s) 90, 216, 548, 638, 667
BclI TGATCA 1 cut(s) 94
BcuI ACTAGT 1 cut(s) 296
BfaI CTAG 4 cut(s) 168, 297, 617, 700
BisI GCNGC 1 cut(s) 697
BlsI GCNGC 1 cut(s) 698
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 5 cut(s) 90, 216, 548, 638, 667
BmgT120I GGNCC 1 cut(s) 663
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 5 cut(s) 90, 216, 548, 638, 667
BmsI GCATC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 663
BplI GAGNNNNNCTC 2 cut(s) 163, 195
Bsa29I ATCGAT 1 cut(s) 655
BsaJI CCNNGG 2 cut(s) 124, 637
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 221, 429
BseBI CCWGG 5 cut(s) 90, 216, 548, 638, 667
BseCI ATCGAT 1 cut(s) 655
BseDI CCNNGG 2 cut(s) 124, 637
BseGI GGATG 3 cut(s) 249, 397, 609
BseLI CCNNNNNNNGG 3 cut(s) 89, 221, 429
BseMII CTCAG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 655
BshFI GGCC 2 cut(s) 422, 665
BshVI ATCGAT 1 cut(s) 655
BsiEI CGRYCG 1 cut(s) 655
BslI CCNNNNNNNGG 3 cut(s) 89, 221, 429
BsnI GGCC 2 cut(s) 422, 665
Bsp143I GATC 4 cut(s) 4, 94, 399, 652
Bsp19I CCATGG 1 cut(s) 124
BspACI CCGC 1 cut(s) 380
BspANI GGCC 2 cut(s) 422, 665
BspCNI CTCAG 1 cut(s) 193
BspDI ATCGAT 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 2 cut(s) 12, 394
BssECI CCNNGG 2 cut(s) 124, 637
BssMI GATC 4 cut(s) 4, 94, 399, 652
BssT1I CCWWGG 1 cut(s) 124
Bst2UI CCWGG 5 cut(s) 90, 216, 548, 638, 667
Bst4CI ACNGT 3 cut(s) 358, 439, 604
BstC8I GCNNGC 2 cut(s) 13, 109
BstDEI CTNAG 1 cut(s) 180
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 3 cut(s) 249, 397, 609
BstKTI GATC 4 cut(s) 7, 97, 402, 655
BstMBI GATC 4 cut(s) 4, 94, 399, 652
BstMCI CGRYCG 1 cut(s) 655
BstMWI GCNNNNNNNGC 2 cut(s) 200, 696
BstNI CCWGG 5 cut(s) 90, 216, 548, 638, 667
BstSCI CCNGG 5 cut(s) 88, 214, 546, 636, 665
BstV1I GCAGC 1 cut(s) 683
BstV2I GAAGAC 1 cut(s) 663
BstXI CCANNNNNNTGG 1 cut(s) 131
Bsu15I ATCGAT 1 cut(s) 655
BsuRI GGCC 2 cut(s) 422, 665
BsuTUI ATCGAT 1 cut(s) 655
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 3 cut(s) 249, 397, 609
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 2 cut(s) 13, 109
Cfr13I GGNCC 1 cut(s) 663
ClaI ATCGAT 1 cut(s) 655
CsiI ACCWGGT 1 cut(s) 88
Csp6I GTAC 3 cut(s) 138, 190, 435
CviAII CATG 4 cut(s) 8, 125, 242, 383
CviQI GTAC 3 cut(s) 138, 190, 435
DdeI CTNAG 1 cut(s) 180
DpnI GATC 4 cut(s) 6, 96, 401, 654
DpnII GATC 4 cut(s) 4, 94, 399, 652
Eco130I CCWWGG 1 cut(s) 124
Eco57I CTGAAG 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 5 cut(s) 88, 214, 546, 636, 665
EcoT14I CCWWGG 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 4 cut(s) 11, 128, 245, 386
FatI CATG 4 cut(s) 7, 124, 241, 382
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 1 cut(s) 697
FokI GGATG 3 cut(s) 236, 404, 596
Fsp4HI GCNGC 1 cut(s) 697
FspBI CTAG 4 cut(s) 168, 297, 617, 700
GluI GCNGC 1 cut(s) 697
HaeIII GGCC 2 cut(s) 422, 665
Hin1II CATG 4 cut(s) 11, 128, 245, 386
HinfI GANTC 2 cut(s) 335, 364
HphI GGTGA 1 cut(s) 104
Hpy166II GTNNAC 2 cut(s) 208, 435
Hpy188I TCNGA 3 cut(s) 20, 232, 517
Hpy188III TCNNGA 2 cut(s) 479, 683
Hpy8I GTNNAC 2 cut(s) 208, 435
HpyAV CCTTC 4 cut(s) 33, 152, 227, 658
HpyCH4III ACNGT 3 cut(s) 358, 439, 604
HpyCH4V TGCA 5 cut(s) 69, 144, 203, 241, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 696
HpyF3I CTNAG 1 cut(s) 180
Hsp92II CATG 4 cut(s) 11, 128, 245, 386
Ksp22I TGATCA 1 cut(s) 94
Kzo9I GATC 4 cut(s) 4, 94, 399, 652
LmnI GCTCC 3 cut(s) 347, 415, 563
Lsp1109I GCAGC 1 cut(s) 683
LweI GCATC 1 cut(s) 24
MabI ACCWGGT 1 cut(s) 88
MaeI CTAG 4 cut(s) 168, 297, 617, 700
MaeIII GTNAC 4 cut(s) 352, 604, 619, 641
MalI GATC 4 cut(s) 6, 96, 401, 654
MboI GATC 4 cut(s) 4, 94, 399, 652
MboII GAAGA 2 cut(s) 142, 663
MluCI AATT 7 cut(s) 43, 60, 174, 290, 447, 472, 629
MlyI GAGTC 1 cut(s) 344
MnlI CCTC 3 cut(s) 19, 511, 679
MroXI GAANNNNTTC 1 cut(s) 43
MslI CAYNNNNRTG 1 cut(s) 129
MspR9I CCNGG 5 cut(s) 90, 216, 548, 638, 667
MvaI CCWGG 5 cut(s) 90, 216, 548, 638, 667
MwoI GCNNNNNNNGC 2 cut(s) 200, 696
NcoI CCATGG 1 cut(s) 124
NdeII GATC 4 cut(s) 4, 94, 399, 652
NlaIII CATG 4 cut(s) 11, 128, 245, 386
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 3 cut(s) 352, 619, 641
PdmI GAANNNNTTC 1 cut(s) 43
PfeI GAWTC 1 cut(s) 364
PflMI CCANNNNNTGG 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 546
PkrI GCNGC 1 cut(s) 698
Ple19I CGATCG 1 cut(s) 655
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PsiI TTATAA 1 cut(s) 326
Psp6I CCWGG 5 cut(s) 88, 214, 546, 636, 665
PspGI CCWGG 5 cut(s) 88, 214, 546, 636, 665
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 663
PvuI CGATCG 1 cut(s) 655
RsaI GTAC 3 cut(s) 139, 191, 436
RsaNI GTAC 3 cut(s) 138, 190, 435
RseI CAYNNNNRTG 1 cut(s) 129
SatI GCNGC 1 cut(s) 697
Sau3AI GATC 4 cut(s) 4, 94, 399, 652
Sau96I GGNCC 1 cut(s) 663
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 5 cut(s) 90, 216, 548, 638, 667
SexAI ACCWGGT 1 cut(s) 88
SfaNI GCATC 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 129
SpeI ACTAGT 1 cut(s) 296
Sse9I AATT 7 cut(s) 43, 60, 174, 290, 447, 472, 629
SsiI CCGC 1 cut(s) 380
SspMI CTAG 4 cut(s) 168, 297, 617, 700
StyD4I CCNGG 5 cut(s) 88, 214, 546, 636, 665
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 3 cut(s) 358, 439, 604
TaqI TCGA 5 cut(s) 273, 338, 458, 651, 655
TasI AATT 7 cut(s) 43, 60, 174, 290, 447, 472, 629
TatI WGTACW 2 cut(s) 137, 434
TfiI GAWTC 1 cut(s) 364
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 3 cut(s) 352, 619, 641
TseI GCWGC 1 cut(s) 696
Tsp45I GTSAC 3 cut(s) 352, 619, 641
TspDTI ATGAA 3 cut(s) 238, 356, 399
TspRI CASTG 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 221
XapI RAATTY 3 cut(s) 43, 60, 447
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 43
XspI CTAG 4 cut(s) 168, 297, 617, 700
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.