Rh4CG360100

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001162
Physical Location & Seq
Forward (+)
61730557 .. 61732763
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG360100.1

Sequence Viewer

Length: 888 bp
ATGAATCTTTTCCTGAGTTTCCGATCACCAGAGGCGTCTCTGTTTCACCGGAGATATAGCTCTCCGATCAAACACCTGCAAAAACAGCAAGTACCCAATTCACTCTCGCTCCCCAAACTACAATTTCCAGTCCCCAAAACTCGGTTCAGGGTCACGGCTCGGAAAGGGTCAGCTATAGAAGGCTTAAGCCAAGAGATGAACACCATCGCCTCGCAGAACCTGGATCACGCGCCTGCTCGGAGACGAGTTCGCTCGGCATTCATCGACGTGCATAAACAACTGGACCATTGCCTGTTCAAGATGGATCATGCTGGCATCAGAACAGAGGAGTGGTATGGAAGGAATTCAAGGGGTATGGAAATTTTCTGCAAAAGCTGGTTGCCAAAACCAGGTGATCAAATCAAAGCTGGCTTATGTTTCTGCCATGGGTACGGGAGTACTTGCACTTTCTTCTTTGAAGGTATTGCTAGGAGAATTGCTCAGTCTGGGTACGCTGTGTATGCAGTAGACTATCCAGGTTTTGGTCTTTCTGAAGGGTTGCATGGTTTCATCCCAGACTTTGATGAGTTGGTCGATGATGTTATTGAACAATTTACTAGTATCAAAGGAAGATCTACTGAAGTGAAAGGTTTGCCCTTTTTTATAATGGGGGAGTCGATGGGTGGAGCTGTCACTCTAAAGATTCATCTAAAGGAACCGCATGAATGGGATGGAGTGATCCTTGTAGCTCCAATGTGTAAAATTGCAGATGATGTGATGCCTCCAGCAATAGTGCTGAAGCTATTAGCCTTCATGTCTGAAGTTTTGCCTCGGGCAAAACTCTTCCCACAGAAAGATATAGCTCATCTAAGCTACAGAGAGCTCGGAAAGAGAAAAACGACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

295

Amino Acids

33.34

Weight (kDa)

9.26

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 133 - 283 2.7e-37 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 138 - 266 2.9e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 140 - 271 1.6e-10 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 644
AarI CACCTGC 1 cut(s) 84
Acc36I ACCTGC 1 cut(s) 84
AccB7I CCANNNNNTGG 1 cut(s) 521
AccI GTMKAC 1 cut(s) 507
AccII CGCG 1 cut(s) 230
AciI CCGC 1 cut(s) 698
AclWI GGATC 3 cut(s) 231, 312, 712
AcsI RAATTY 2 cut(s) 343, 360
AcuI CTGAAG 4 cut(s) 552, 639, 797, 819
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 4 cut(s) 93, 431, 439, 491
AfiI CCNNNNNNNGG 3 cut(s) 141, 389, 521
AflII CTTAAG 1 cut(s) 184
AgsI TTSAA 4 cut(s) 298, 348, 458, 587
AhlI ACTAGT 1 cut(s) 596
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 3 cut(s) 219, 388, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 2 cut(s) 42, 235
AlwI GGATC 3 cut(s) 231, 312, 712
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 810
ApoI RAATTY 2 cut(s) 343, 360
Asp700I GAANNNNTTC 2 cut(s) 8, 343
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 3 cut(s) 18, 38, 404
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 1 cut(s) 283
BaeI ACNNNNGTAYC 2 cut(s) 421, 454
BanII GRGCYC 1 cut(s) 864
Bbv12I GWGCWC 1 cut(s) 864
BccI CCATC 4 cut(s) 212, 295, 652, 704
BceAI ACGGC 1 cut(s) 171
BciT130I CCWGG 3 cut(s) 221, 390, 516
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 42, 235
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 2 cut(s) 468, 597
BfmI CTRYAG 2 cut(s) 174, 853
BfrI CTTAAG 1 cut(s) 184
BfuAI ACCTGC 1 cut(s) 84
BglII AGATCT 1 cut(s) 611
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 3 cut(s) 221, 390, 516
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 810
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 1 cut(s) 696
BmrFI CCNGG 3 cut(s) 221, 390, 516
BmsI GCATC 2 cut(s) 324, 747
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 1 cut(s) 747
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 424, 809
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 389, 521
Bse1I ACTGG 2 cut(s) 128, 285
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 3 cut(s) 221, 390, 516
BseDI CCNNGG 2 cut(s) 424, 809
BseGI GGATG 2 cut(s) 549, 715
BseLI CCNNNNNNNGG 3 cut(s) 141, 389, 521
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 5, 494
BseNI ACTGG 2 cut(s) 128, 285
BseRI GAGGAG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 3 cut(s) 141, 389, 521
BsmAI GTCTC 2 cut(s) 42, 235
BsmBI CGTCTC 2 cut(s) 42, 235
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 1 cut(s) 864
Bsp143I GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 1 cut(s) 698
BspCNI CTCAG 2 cut(s) 6, 493
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 696
BspMI ACCTGC 1 cut(s) 84
BspPI GGATC 3 cut(s) 231, 312, 712
BspTI CTTAAG 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 286
BsrI ACTGG 2 cut(s) 128, 285
BssECI CCNNGG 2 cut(s) 424, 809
BssMI GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 3 cut(s) 221, 390, 516
Bst6I CTCTTC 1 cut(s) 827
BstACI GRCGYC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 234, 313, 409
BstDEI CTNAG 3 cut(s) 14, 480, 848
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 2 cut(s) 549, 715
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 7 cut(s) 26, 69, 226, 307, 397, 614, 720
BstMAI GTCTC 2 cut(s) 42, 235
BstMBI GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
BstMWI GCNNNNNNNGC 2 cut(s) 85, 500
BstNI CCWGG 3 cut(s) 221, 390, 516
BstSCI CCNGG 3 cut(s) 219, 388, 514
BstSFI CTRYAG 2 cut(s) 174, 853
BstUI CGCG 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 611
BstYI RGATCY 1 cut(s) 611
BtgI CCRYGG 1 cut(s) 424
BtgZI GCGATG 1 cut(s) 190
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 2 cut(s) 549, 715
BveI ACCTGC 1 cut(s) 84
Cac8I GCNNGC 3 cut(s) 234, 313, 409
CaiI CAGNNNCTG 1 cut(s) 220
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 388
Csp6I GTAC 4 cut(s) 92, 430, 438, 490
CviAII CATG 5 cut(s) 308, 425, 542, 701, 793
CviQI GTAC 4 cut(s) 92, 430, 438, 490
DdeI CTNAG 3 cut(s) 14, 480, 848
DpnI GATC 7 cut(s) 25, 68, 225, 306, 396, 613, 719
DpnII GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
Ecl136II GAGCTC 1 cut(s) 862
Eco130I CCWWGG 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 864
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 862
Eco57I CTGAAG 4 cut(s) 552, 639, 797, 819
Eco88I CYCGRG 1 cut(s) 810
EcoICRI GAGCTC 1 cut(s) 862
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 3 cut(s) 219, 388, 514
EcoT14I CCWWGG 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 864
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 2 cut(s) 42, 235
FaeI CATG 5 cut(s) 311, 428, 545, 704, 796
FaqI GGGAC 1 cut(s) 116
FatI CATG 5 cut(s) 307, 424, 541, 700, 792
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 507
FokI GGATG 2 cut(s) 536, 722
FriOI GRGCYC 1 cut(s) 864
FspBI CTAG 2 cut(s) 468, 597
GlaI GCGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 747
HapII CCGG 1 cut(s) 49
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 232
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 5 cut(s) 311, 428, 545, 704, 796
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 4 cut(s) 4, 653, 682, 880
HpaII CCGG 1 cut(s) 49
HphI GGTGA 3 cut(s) 18, 38, 404
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 8 cut(s) 23, 66, 162, 240, 320, 532, 799, 866
Hpy188III TCNNGA 2 cut(s) 13, 298
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 5 cut(s) 173, 333, 452, 527, 799
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 7 cut(s) 79, 271, 369, 444, 503, 541, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 500
HpyF3I CTNAG 3 cut(s) 14, 480, 848
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 5 cut(s) 311, 428, 545, 704, 796
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
LmnI GCTCC 3 cut(s) 114, 665, 733
LweI GCATC 2 cut(s) 324, 747
MabI ACCWGGT 1 cut(s) 388
MaeI CTAG 2 cut(s) 468, 597
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 151, 670
MalI GATC 7 cut(s) 25, 68, 225, 306, 396, 613, 719
MboI GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
MboII GAAGA 3 cut(s) 442, 621, 814
MflI RGATCY 1 cut(s) 611
MhlI GDGCHC 1 cut(s) 864
MluCI AATT 7 cut(s) 97, 122, 343, 360, 474, 590, 741
MlyI GAGTC 2 cut(s) 662, 874
MnlI CCTC 5 cut(s) 25, 220, 319, 771, 819
MroXI GAANNNNTTC 2 cut(s) 8, 343
MseI TTAA 1 cut(s) 185
MslI CAYNNNNRTG 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 184
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 3 cut(s) 221, 390, 516
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 3 cut(s) 221, 390, 516
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 2 cut(s) 85, 500
NcoI CCATGG 1 cut(s) 424
NdeII GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
NlaIII CATG 5 cut(s) 311, 428, 545, 704, 796
NlaIV GGNNCC 1 cut(s) 696
NmeAIII GCCGAG 1 cut(s) 233
NmuCI GTSAC 2 cut(s) 151, 670
PaqCI CACCTGC 1 cut(s) 84
PctI GAATGC 1 cut(s) 257
PdmI GAANNNNTTC 2 cut(s) 8, 343
PfeI GAWTC 2 cut(s) 4, 682
PflMI CCANNNNNTGG 1 cut(s) 521
PleI GAGTC 2 cut(s) 661, 874
PpsI GAGTC 2 cut(s) 661, 874
PsiI TTATAA 1 cut(s) 644
Psp124BI GAGCTC 1 cut(s) 864
Psp6I CCWGG 3 cut(s) 219, 388, 514
PspGI CCWGG 3 cut(s) 219, 388, 514
PspN4I GGNNCC 1 cut(s) 696
PspPI GGNCC 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 1 cut(s) 611
RsaI GTAC 4 cut(s) 93, 431, 439, 491
RsaNI GTAC 4 cut(s) 92, 430, 438, 490
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 864
SaqAI TTAA 1 cut(s) 185
Sau3AI GATC 7 cut(s) 23, 66, 223, 304, 394, 611, 717
Sau96I GGNCC 1 cut(s) 283
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 2 cut(s) 662, 874
ScrFI CCNGG 3 cut(s) 221, 390, 516
SduI GDGCHC 1 cut(s) 864
SexAI ACCWGGT 1 cut(s) 388
SfaNI GCATC 2 cut(s) 324, 747
SfcI CTRYAG 2 cut(s) 174, 853
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 7 cut(s) 97, 122, 343, 360, 474, 590, 741
SsiI CCGC 1 cut(s) 698
SspMI CTAG 2 cut(s) 468, 597
SstI GAGCTC 1 cut(s) 864
StyD4I CCNGG 3 cut(s) 219, 388, 514
StyI CCWWGG 1 cut(s) 424
TaiI ACGT 1 cut(s) 270
TaqI TCGA 3 cut(s) 264, 573, 656
TasI AATT 7 cut(s) 97, 122, 343, 360, 474, 590, 741
TatI WGTACW 1 cut(s) 437
TfiI GAWTC 2 cut(s) 4, 682
Tru1I TTAA 1 cut(s) 185
Tru9I TTAA 1 cut(s) 185
TseFI GTSAC 2 cut(s) 151, 670
Tsp45I GTSAC 2 cut(s) 151, 670
TspDTI ATGAA 7 cut(s) 17, 212, 250, 538, 674, 717, 781
Van91I CCANNNNNTGG 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 2 cut(s) 343, 360
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 2 cut(s) 8, 343
XspI CTAG 2 cut(s) 468, 597
ZrmI AGTACT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.