MD16G1042000.v1.1

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001162
Physical Location & Seq
Forward (+)
2958014 .. 2960730
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1042000.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGACTTTTCTCGCTCCCTGAGACTCCAAACTCTCGAGCTTCCTCTCAGATTCCAAACCCCAATCGACCACCTCCACCAAAACCCCACATTCTCCGTCCCCGGAGGAGCTCGCGGCCGGATTGTTTCAGCCTTGAGAAGGCCACCCATTGAAGGTTTGAGCGAGGAGCTGAACTCGATCGCCAGGCAGAACCTCGACTTTGCCCGCACTCGCCGGCAGGTTCGATCCGCTTTCGTTGAAGTTCAGGAACAGCTCGATCATGTCCTGTTTAAGTTTCCAACTGGGATCAGAACAGAGGAGTGGTACGAAAGGAATTCTAGGGGGGTGGAGATTTTCTGTAAAAGCTGGATGCCGAAACAAGGTGTTCGAATTAAAGGTGTAATGTGTTTTTGTCACGGATATGGTGATACTTGCGTTTTCTTCTTTGAAGGTATTGCCAGGCGAATTGCTGCAGCTGGCTACGCTGTATATGCCATGGACTATCCGGGTTTTGGTCTTTCAGAAGGATTGCATGGTTACGTCCCAAGCTTTGATCAATTAGCTGATGATGTGATCGAACAGTACACCAAGATTAAAGAAAGACCGGAGTTGAAAGGGTTGCCCCGCTTTATATTGGGGCAGTCCATGGGTGGTGCCGTTACTCTCAAAGTGCACCTAAAGGAACCATATGCATGGGATGGAGTAGTTCTTGTGGCACCAATGTGTAAAATTGCAGAAGACGTAACACCTCCAGCAGCAGCTCTGAAGATATTAACTCTCATGTCAAAAGTTATGCCGAAAGCAAAGCTTGTCCCACAGAAAGATTTAGCGGAGCTGGCCTTCAGAGACCTTCGGAAACGGGAAATGGCTGTCTACAATGTGATATGCTACAACGACAATGTGCGGTTGAAGACTGCTGTGGAACTGCTACAAGCTACTAAGGATATTGAGATGCAACTCGAGAAGGTATCAGCGCCACTCCTAGTTCTTCATGGAGCTGCAGACAAGGTGACTGATCCTCGGGTGAGCCAGTTTCTTTATGAGAAGGCCTCTAGCAAGGATAAAACCCTAAAACTCTACCCGGAAGGTTATCATTCCATTCTCGAAGGAGAACCCGATGACAGAATTCTTACCGTCCTTGACGATATTGTCACTTGGCTGGATTCCCGCGTTCCCCTACATAGCTTTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

392

Amino Acids

44.23

Weight (kDa)

8.11

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 124 - 365 2.3e-68 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 127 - 364 2.5e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 130 - 368 1.1e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1127
Acc36I ACCTGC 1 cut(s) 208
AccB1I GGYRCC 2 cut(s) 632, 694
AccI GTMKAC 1 cut(s) 852
AccII CGCG 2 cut(s) 114, 1149
AciI CCGC 7 cut(s) 114, 205, 228, 604, 809, 883, 1147
AclWI GGATC 3 cut(s) 219, 293, 989
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 2 cut(s) 313, 1104
AcuI CTGAAG 2 cut(s) 764, 805
AfaI GTAC 2 cut(s) 305, 563
AfiI CCNNNNNNNGG 4 cut(s) 138, 152, 359, 491
AgsI TTSAA 5 cut(s) 152, 239, 428, 592, 889
AjnI CCWGG 2 cut(s) 182, 437
AleI CACNNNNGTG 1 cut(s) 700
Alw21I GWGCWC 2 cut(s) 112, 654
Alw26I GTCTC 2 cut(s) 16, 819
Alw44I GTGCAC 1 cut(s) 650
AlwI GGATC 3 cut(s) 219, 293, 989
Ama87I CYCGRG 3 cut(s) 35, 938, 999
AoxI GGCC 4 cut(s) 115, 140, 816, 1026
ApaLI GTGCAC 1 cut(s) 650
ApeKI GCWGC 5 cut(s) 449, 452, 734, 737, 977
ApoI RAATTY 2 cut(s) 313, 1104
AspLEI GCGC 1 cut(s) 955
AsuC2I CCSGG 3 cut(s) 102, 486, 1061
AsuHPI GGTGA 3 cut(s) 416, 1000, 1015
AsuII TTCGAA 1 cut(s) 367
AvaI CYCGRG 3 cut(s) 35, 938, 999
BaeGI GKGCMC 1 cut(s) 654
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanI GGYRCC 2 cut(s) 632, 694
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 723, 896
Bbv12I GWGCWC 2 cut(s) 112, 654
BbvI GCAGC 5 cut(s) 436, 464, 746, 749, 964
BccI CCATC 1 cut(s) 671
BceAI ACGGC 1 cut(s) 620
BciT130I CCWGG 2 cut(s) 184, 439
BclI TGATCA 1 cut(s) 532
BcnI CCSGG 3 cut(s) 102, 486, 1061
BcoDI GTCTC 2 cut(s) 16, 819
BfaI CTAG 3 cut(s) 318, 962, 1032
BfmI CTRYAG 2 cut(s) 450, 978
BfoI RGCGCY 1 cut(s) 956
BfuAI ACCTGC 1 cut(s) 208
BisI GCNGC 6 cut(s) 115, 450, 453, 735, 738, 978
BlsI GCNGC 6 cut(s) 116, 451, 454, 736, 739, 979
Bme1390I CCNGG 5 cut(s) 102, 184, 439, 486, 1061
BmeT110I CYCGRG 3 cut(s) 35, 938, 999
BmiI GGNNCC 3 cut(s) 634, 663, 696
BmrFI CCNGG 5 cut(s) 102, 184, 439, 486, 1061
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 2 cut(s) 339, 921
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 723, 896
BplI GAGNNNNNCTC 4 cut(s) 158, 190, 1013, 1045
BpmI CTGGAG 1 cut(s) 714
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 3 cut(s) 102, 486, 1061
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 4 cut(s) 100, 474, 624, 998
BsaWI WCCGGW 1 cut(s) 583
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 152, 359, 491
Bse118I RCCGGY 1 cut(s) 213
Bse1I ACTGG 2 cut(s) 286, 1009
BseBI CCWGG 2 cut(s) 184, 439
BseDI CCNNGG 4 cut(s) 100, 474, 624, 998
BseGI GGATG 2 cut(s) 354, 682
BseLI CCNNNNNNNGG 4 cut(s) 138, 152, 359, 491
BseMII CTCAG 2 cut(s) 11, 61
BseNI ACTGG 2 cut(s) 286, 1009
BseRI GAGGAG 3 cut(s) 120, 179, 311
BseSI GKGCMC 1 cut(s) 654
BseX3I CGGCCG 1 cut(s) 115
BseXI GCAGC 5 cut(s) 436, 464, 746, 749, 964
Bsh1236I CGCG 2 cut(s) 114, 1149
Bsh1285I CGRYCG 2 cut(s) 118, 180
BshFI GGCC 4 cut(s) 117, 142, 818, 1028
BshNI GGYRCC 2 cut(s) 632, 694
BsiEI CGRYCG 2 cut(s) 118, 180
BsiHKAI GWGCWC 2 cut(s) 112, 654
BsiHKCI CYCGRG 3 cut(s) 35, 938, 999
BsiSI CCGG 6 cut(s) 102, 118, 214, 485, 584, 1061
BslFI GGGAC 3 cut(s) 83, 506, 776
BslI CCNNNNNNNGG 4 cut(s) 138, 152, 359, 491
BsmAI GTCTC 2 cut(s) 16, 819
BsmFI GGGAC 3 cut(s) 83, 506, 776
BsnI GGCC 4 cut(s) 117, 142, 818, 1028
Bso31I GGTCTC 1 cut(s) 819
BsoBI CYCGRG 3 cut(s) 35, 938, 999
Bsp119I TTCGAA 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 112, 654
Bsp143I GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
Bsp19I CCATGG 2 cut(s) 474, 624
BspACI CCGC 7 cut(s) 114, 205, 228, 604, 809, 883, 1147
BspANI GGCC 4 cut(s) 117, 142, 818, 1028
BspCNI CTCAG 2 cut(s) 12, 60
BspFNI CGCG 2 cut(s) 114, 1149
BspLI GGNNCC 3 cut(s) 634, 663, 696
BspMAI CTGCAG 2 cut(s) 454, 982
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 3 cut(s) 219, 293, 989
BspT104I TTCGAA 1 cut(s) 367
BspT107I GGYRCC 2 cut(s) 632, 694
BspTNI GGTCTC 1 cut(s) 819
BsrFI RCCGGY 1 cut(s) 213
BsrI ACTGG 2 cut(s) 286, 1009
BssAI RCCGGY 1 cut(s) 213
BssECI CCNNGG 4 cut(s) 100, 474, 624, 998
BssMI GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
BssT1I CCWWGG 2 cut(s) 474, 624
Bst2UI CCWGG 2 cut(s) 184, 439
Bst4CI ACNGT 2 cut(s) 561, 1114
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 5 cut(s) 112, 205, 215, 457, 816
BstDEI CTNAG 3 cut(s) 20, 47, 918
BstDSI CCRYGG 2 cut(s) 474, 624
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 2 cut(s) 354, 682
BstFNI CGCG 2 cut(s) 114, 1149
BstH2I RGCGCY 1 cut(s) 956
BstHHI GCGC 1 cut(s) 955
BstKTI GATC 7 cut(s) 180, 227, 259, 288, 535, 555, 997
BstMAI GTCTC 2 cut(s) 16, 819
BstMBI GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
BstMCI CGRYCG 2 cut(s) 118, 180
BstMWI GCNNNNNNNGC 3 cut(s) 461, 470, 815
BstNI CCWGG 2 cut(s) 184, 439
BstSCI CCNGG 5 cut(s) 100, 182, 437, 484, 1059
BstSFI CTRYAG 2 cut(s) 450, 978
BstSLI GKGCMC 1 cut(s) 654
BstUI CGCG 2 cut(s) 114, 1149
BstV1I GCAGC 5 cut(s) 436, 464, 746, 749, 964
BstV2I GAAGAC 2 cut(s) 723, 896
BstXI CCANNNNNNTGG 1 cut(s) 672
BstZI CGGCCG 1 cut(s) 115
BsuRI GGCC 4 cut(s) 117, 142, 818, 1028
BtgI CCRYGG 2 cut(s) 474, 624
BtsCI GGATG 2 cut(s) 354, 682
BveI ACCTGC 1 cut(s) 208
Cac8I GCNNGC 5 cut(s) 112, 205, 215, 457, 816
CfoI GCGC 1 cut(s) 955
Cfr10I RCCGGY 1 cut(s) 213
Csp6I GTAC 2 cut(s) 304, 562
CviAII CATG 7 cut(s) 260, 475, 512, 625, 672, 760, 971
CviQI GTAC 2 cut(s) 304, 562
DdeI CTNAG 3 cut(s) 20, 47, 918
DpnI GATC 7 cut(s) 179, 226, 258, 287, 534, 554, 996
DpnII GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
DrdI GACNNNNNNGTC 1 cut(s) 1127
DseDI GACNNNNNNGTC 1 cut(s) 1127
EaeI YGGCCR 1 cut(s) 115
EagI CGGCCG 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 110
EclXI CGGCCG 1 cut(s) 115
Eco130I CCWWGG 2 cut(s) 474, 624
Eco147I AGGCCT 1 cut(s) 1028
Eco24I GRGCYC 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 819
Eco52I CGGCCG 1 cut(s) 115
Eco53kI GAGCTC 1 cut(s) 110
Eco57I CTGAAG 2 cut(s) 764, 805
Eco88I CYCGRG 3 cut(s) 35, 938, 999
EcoICRI GAGCTC 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoRI GAATTC 2 cut(s) 313, 1104
EcoRII CCWGG 2 cut(s) 182, 437
EcoT14I CCWWGG 2 cut(s) 474, 624
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 2 cut(s) 474, 624
FaeI CATG 7 cut(s) 263, 478, 515, 628, 675, 763, 974
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 3 cut(s) 83, 506, 776
FatI CATG 7 cut(s) 259, 474, 511, 624, 671, 759, 970
FauI CCCGC 3 cut(s) 212, 611, 1154
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 852
Fnu4HI GCNGC 6 cut(s) 115, 450, 453, 735, 738, 978
FokI GGATG 2 cut(s) 361, 689
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 6 cut(s) 115, 450, 453, 735, 738, 978
FspBI CTAG 3 cut(s) 318, 962, 1032
GlaI GCGC 1 cut(s) 954
GluI GCNGC 6 cut(s) 115, 450, 453, 735, 738, 978
GsuI CTGGAG 1 cut(s) 714
HaeII RGCGCY 1 cut(s) 956
HaeIII GGCC 4 cut(s) 117, 142, 818, 1028
HapII CCGG 6 cut(s) 102, 118, 214, 485, 584, 1061
HhaI GCGC 1 cut(s) 955
Hin1II CATG 7 cut(s) 263, 478, 515, 628, 675, 763, 974
Hin6I GCGC 1 cut(s) 953
HinP1I GCGC 1 cut(s) 953
HindIII AAGCTT 2 cut(s) 526, 785
HinfI GANTC 3 cut(s) 24, 51, 1142
HpaII CCGG 6 cut(s) 102, 118, 214, 485, 584, 1061
HphI GGTGA 3 cut(s) 416, 1000, 1015
Hpy166II GTNNAC 3 cut(s) 564, 652, 853
Hpy188I TCNGA 7 cut(s) 50, 290, 502, 744, 824, 834, 1175
Hpy188III TCNNGA 4 cut(s) 35, 245, 940, 1082
Hpy8I GTNNAC 3 cut(s) 564, 652, 853
HpyCH4III ACNGT 2 cut(s) 561, 1114
HpyCH4IV ACGT 2 cut(s) 519, 720
HpyCH4V TGCA 7 cut(s) 452, 511, 652, 671, 713, 934, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 461, 470, 815
HpyF3I CTNAG 3 cut(s) 20, 47, 918
HpySE526I ACGT 2 cut(s) 519, 720
Hsp92II CATG 7 cut(s) 263, 478, 515, 628, 675, 763, 974
HspAI GCGC 1 cut(s) 953
KroI GCCGGC 1 cut(s) 213
KroNI GCCGGC 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
LmnI GCTCC 5 cut(s) 20, 107, 166, 811, 974
Lsp1109I GCAGC 5 cut(s) 436, 464, 746, 749, 964
LweI GCATC 2 cut(s) 339, 921
MaeI CTAG 3 cut(s) 318, 962, 1032
MaeII ACGT 2 cut(s) 519, 720
MaeIII GTNAC 6 cut(s) 392, 515, 637, 721, 988, 1129
MalI GATC 7 cut(s) 179, 226, 258, 287, 534, 554, 996
MboI GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
MboII GAAGA 5 cut(s) 412, 728, 757, 901, 959
MhlI GDGCHC 2 cut(s) 112, 654
MluCI AATT 6 cut(s) 313, 369, 444, 536, 708, 1104
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 9 cut(s) 54, 83, 98, 157, 203, 289, 738, 1008, 1039
Mph1103I ATGCAT 1 cut(s) 673
MroNI GCCGGC 1 cut(s) 213
MseI TTAA 4 cut(s) 270, 372, 573, 752
MslI CAYNNNNRTG 3 cut(s) 399, 670, 700
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 6 cut(s) 102, 118, 214, 485, 584, 1061
MspR9I CCNGG 5 cut(s) 102, 184, 439, 486, 1061
MvaI CCWGG 2 cut(s) 184, 439
MvnI CGCG 2 cut(s) 114, 1149
MwoI GCNNNNNNNGC 3 cut(s) 461, 470, 815
NaeI GCCGGC 1 cut(s) 215
NciI CCSGG 3 cut(s) 102, 486, 1061
NcoI CCATGG 2 cut(s) 474, 624
NdeI CATATG 1 cut(s) 667
NdeII GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
NgoMIV GCCGGC 1 cut(s) 213
NlaIII CATG 7 cut(s) 263, 478, 515, 628, 675, 763, 974
NlaIV GGNNCC 3 cut(s) 634, 663, 696
NmuCI GTSAC 3 cut(s) 392, 988, 1129
NsiI ATGCAT 1 cut(s) 673
NspV TTCGAA 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 700
PaeR7I CTCGAG 2 cut(s) 35, 938
PceI AGGCCT 1 cut(s) 1028
PdiI GCCGGC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 51, 1142
PkrI GCNGC 6 cut(s) 116, 451, 454, 736, 739, 979
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
Psp124BI GAGCTC 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 182, 437
PspGI CCWGG 2 cut(s) 182, 437
PspN4I GGNNCC 3 cut(s) 634, 663, 696
PstI CTGCAG 2 cut(s) 454, 982
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 2 cut(s) 305, 563
RsaNI GTAC 2 cut(s) 304, 562
RseI CAYNNNNRTG 3 cut(s) 399, 670, 700
SacI GAGCTC 1 cut(s) 112
SaqAI TTAA 4 cut(s) 270, 372, 573, 752
SatI GCNGC 6 cut(s) 115, 450, 453, 735, 738, 978
Sau3AI GATC 7 cut(s) 177, 224, 256, 285, 532, 552, 994
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 5 cut(s) 102, 184, 439, 486, 1061
SduI GDGCHC 2 cut(s) 112, 654
SfaNI GCATC 2 cut(s) 339, 921
SfcI CTRYAG 2 cut(s) 450, 978
Sfr274I CTCGAG 2 cut(s) 35, 938
SfuI TTCGAA 1 cut(s) 367
SlaI CTCGAG 2 cut(s) 35, 938
SmiMI CAYNNNNRTG 3 cut(s) 399, 670, 700
SmlI CTYRAG 3 cut(s) 35, 133, 938
SmoI CTYRAG 3 cut(s) 35, 133, 938
Sse9I AATT 6 cut(s) 313, 369, 444, 536, 708, 1104
SseBI AGGCCT 1 cut(s) 1028
SsiI CCGC 7 cut(s) 114, 205, 228, 604, 809, 883, 1147
SspMI CTAG 3 cut(s) 318, 962, 1032
SstI GAGCTC 1 cut(s) 112
StuI AGGCCT 1 cut(s) 1028
StyD4I CCNGG 5 cut(s) 100, 182, 437, 484, 1059
StyI CCWWGG 2 cut(s) 474, 624
TaaI ACNGT 2 cut(s) 561, 1114
TaiI ACGT 2 cut(s) 522, 723
TasI AATT 6 cut(s) 313, 369, 444, 536, 708, 1104
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 2 cut(s) 51, 1142
Tru1I TTAA 4 cut(s) 270, 372, 573, 752
Tru9I TTAA 4 cut(s) 270, 372, 573, 752
TseFI GTSAC 3 cut(s) 392, 988, 1129
TseI GCWGC 5 cut(s) 449, 452, 734, 737, 977
Tsp45I GTSAC 3 cut(s) 392, 988, 1129
TspDTI ATGAA 1 cut(s) 959
TspGWI ACGGA 2 cut(s) 85, 411
VneI GTGCAC 1 cut(s) 650
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 2 cut(s) 313, 1104
XhoI CTCGAG 2 cut(s) 35, 938
XmiI GTMKAC 1 cut(s) 852
XspI CTAG 3 cut(s) 318, 962, 1032
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.