Rorug04G0282100

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001162
Physical Location & Seq
Forward (+)
45556137 .. 45557423
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0282100.1

Sequence Viewer

Length: 882 bp
ATGGGGGGCCTTCTGGTGAGTATTTTGTTGACTCCCTTTGCACAATTTTTGGGGAGATCATGGAATTTGGTAGACATAATTAGAGCGGCCACTTTTCTGGCCCCCCACGTCCTCTGTTTGTTTGCTCCGTTTTATTTCACATGGGCTGCCTTTTGGGTGGCCGCCGCACTCTATATTCTCACTGGTTTGGGAGTCACTCTGGGTTTCCATAGACACCTTGCGCACAAGAGTTTACGGCTTCCCAGATGGCTTGAGTACTTGTTTGCCTATTTCGGGGCTCTATCACTTCAGGGAAGTCCGATAGAATGGGTGAGCACGCACCGATACCATCACCAGTTTACAGACACAGAGAGAGACCCTCACAGCCCCTTGAAAGGTTTCTGGTTTAGTCATGTGGGTTGGATCTTTGATAGCACTTCTCGATATCGAAAAAATGGAGGATTGAAGAACGTTGAGGATATGAAGAAGCAGTGGTTCTATAGATTTCTTCATCGTACATACCTTTTACACATAATTGTTTCTGGAGCTTCGCTCTATGCCCTAGGTGGACTACCTTTCTTTATTTGGGGCATGGGAGTGAGGACGGTATATGTTTTCCACTTCACCTTGCTAGTAAACTCGGCCGGTCACATCTGGGGAAAACAAGTATGGAACACCGGCGACCTGTCAAAGAACAATTGGTGGTTGGGATGGATCGCACTTGGAGAAGGGTGGCACAACAACCATCATGCATTTGAATACTCAGCTCGACAAGGTCTGGAATGGTGGCAAGTCGACTTGACTTGGTATGTAATCACAGCTCTTCAAGCACTTGGGTTGGCAACTGACGTCAGAGTTCCCACTGAATCTCAGATGCGTCGCAAAGCCCTCAAGAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

293

Amino Acids

34.05

Weight (kDa)

9.78

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 47 - 265 3e-19 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 831
Acc16I TGCGCA 1 cut(s) 222
AccBSI CCGCTC 1 cut(s) 86
AccI GTMKAC 2 cut(s) 72, 774
AciI CCGC 3 cut(s) 86, 162, 165
AclI AACGTT 1 cut(s) 450
AclWI GGATC 2 cut(s) 410, 701
AcoI YGGCCR 3 cut(s) 87, 159, 621
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 828
AfaI GTAC 2 cut(s) 257, 496
AfiI CCNNNNNNNGG 2 cut(s) 273, 374
AgsI TTSAA 4 cut(s) 373, 445, 737, 806
AjiI CACGTC 1 cut(s) 109
AluBI AGCT 3 cut(s) 527, 746, 800
AluI AGCT 3 cut(s) 527, 746, 800
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 2 cut(s) 410, 701
AoxI GGCC 5 cut(s) 7, 87, 99, 159, 621
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 1 cut(s) 64
Asp700I GAANNNNTTC 1 cut(s) 377
AspA2I CCTAGG 1 cut(s) 541
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 2 cut(s) 7, 100
AsuHPI GGTGA 4 cut(s) 28, 322, 323, 595
AvrII CCTAGG 1 cut(s) 541
BanII GRGCYC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 317
BbvI GCAGC 1 cut(s) 133
BccI CCATC 4 cut(s) 240, 336, 684, 732
BceAI ACGGC 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 2 cut(s) 542, 611
BfmI CTRYAG 1 cut(s) 478
BisI GCNGC 4 cut(s) 87, 147, 162, 165
BlnI CCTAGG 1 cut(s) 541
BlsI GCNGC 4 cut(s) 88, 148, 163, 166
BmcAI AGTACT 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 7, 100
BmiI GGNNCC 2 cut(s) 8, 102
BmsI GCATC 1 cut(s) 843
BplI GAGNNNNNCTC 4 cut(s) 343, 375, 516, 548
BpmI CTGGAG 1 cut(s) 543
BpuEI CTTGAG 2 cut(s) 272, 854
BsaHI GRCGYC 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 541
Bsc4I CCNNNNNNNGG 2 cut(s) 273, 374
Bse118I RCCGGY 2 cut(s) 623, 656
Bse1I ACTGG 2 cut(s) 187, 334
BseDI CCNNGG 1 cut(s) 541
BseGI GGATG 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 273, 374
BseMII CTCAG 2 cut(s) 756, 863
BseNI ACTGG 2 cut(s) 187, 334
BseX3I CGGCCG 1 cut(s) 621
BseXI GCAGC 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 624
BshFI GGCC 5 cut(s) 9, 89, 101, 161, 623
BsiEI CGRYCG 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 317
BsiSI CCGG 2 cut(s) 624, 657
BslI CCNNNNNNNGG 2 cut(s) 273, 374
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 5 cut(s) 9, 89, 101, 161, 623
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 280, 317
Bsp143I GATC 3 cut(s) 56, 402, 693
BspACI CCGC 3 cut(s) 86, 162, 165
BspANI GGCC 5 cut(s) 9, 89, 101, 161, 623
BspCNI CTCAG 2 cut(s) 755, 862
BspLI GGNNCC 2 cut(s) 8, 102
BspPI GGATC 2 cut(s) 410, 701
BspQI GCTCTTC 1 cut(s) 807
BspTNI GGTCTC 1 cut(s) 348
BsrBI CCGCTC 1 cut(s) 86
BsrFI RCCGGY 2 cut(s) 623, 656
BsrI ACTGG 2 cut(s) 187, 334
BssAI RCCGGY 2 cut(s) 623, 656
BssECI CCNNGG 1 cut(s) 541
BssMI GATC 3 cut(s) 56, 402, 693
BssNI GRCGYC 1 cut(s) 828
BssT1I CCWWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 1 cut(s) 828
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 2 cut(s) 742, 849
BstF5I GGATG 1 cut(s) 695
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 3 cut(s) 59, 405, 696
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 3 cut(s) 56, 402, 693
BstMCI CGRYCG 1 cut(s) 624
BstMWI GCNNNNNNNGC 1 cut(s) 806
BstSFI CTRYAG 1 cut(s) 478
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 402
BstXI CCANNNNNNTGG 1 cut(s) 97
BstYI RGATCY 1 cut(s) 402
BstZI CGGCCG 1 cut(s) 621
BsuRI GGCC 5 cut(s) 9, 89, 101, 161, 623
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 695
BtsI GCAGTG 1 cut(s) 476
BtsIMutI CAGTG 3 cut(s) 180, 476, 840
Cac8I GCNNGC 1 cut(s) 317
CfoI GCGC 1 cut(s) 223
Cfr10I RCCGGY 2 cut(s) 623, 656
Cfr13I GGNCC 2 cut(s) 7, 100
CseI GACGC 1 cut(s) 845
Csp6I GTAC 2 cut(s) 256, 495
CviAII CATG 5 cut(s) 60, 141, 392, 571, 728
CviQI GTAC 2 cut(s) 256, 495
DdeI CTNAG 2 cut(s) 742, 849
DpnI GATC 3 cut(s) 58, 404, 695
DpnII GATC 3 cut(s) 56, 402, 693
EaeI YGGCCR 3 cut(s) 87, 159, 621
EagI CGGCCG 1 cut(s) 621
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
EclXI CGGCCG 1 cut(s) 621
Eco130I CCWWGG 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 348
Eco32I GATATC 1 cut(s) 425
Eco52I CGGCCG 1 cut(s) 621
Eco57I CTGAAG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 7
EcoRV GATATC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 541
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 541
FaeI CATG 5 cut(s) 63, 144, 395, 574, 731
FatI CATG 5 cut(s) 59, 140, 391, 570, 727
FblI GTMKAC 2 cut(s) 72, 774
Fnu4HI GCNGC 4 cut(s) 87, 147, 162, 165
FokI GGATG 1 cut(s) 702
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 4 cut(s) 87, 147, 162, 165
FspBI CTAG 2 cut(s) 542, 611
FspI TGCGCA 1 cut(s) 222
GlaI GCGC 1 cut(s) 222
GluI GCNGC 4 cut(s) 87, 147, 162, 165
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 5 cut(s) 9, 89, 101, 161, 623
HapII CCGG 2 cut(s) 624, 657
HgaI GACGC 1 cut(s) 845
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 828
Hin1II CATG 5 cut(s) 63, 144, 395, 574, 731
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 2 cut(s) 30, 775
HindII GTYRAC 2 cut(s) 30, 775
HinfI GANTC 3 cut(s) 31, 192, 845
HpaII CCGG 2 cut(s) 624, 657
HphI GGTGA 4 cut(s) 28, 322, 323, 595
Hpy166II GTNNAC 7 cut(s) 30, 73, 233, 339, 548, 616, 775
Hpy188I TCNGA 3 cut(s) 300, 833, 852
Hpy188III TCNNGA 4 cut(s) 420, 522, 758, 871
Hpy8I GTNNAC 7 cut(s) 30, 73, 233, 339, 548, 616, 775
Hpy99I CGWCG 1 cut(s) 861
HpyAV CCTTC 2 cut(s) 20, 701
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 3 cut(s) 108, 450, 828
HpyCH4V TGCA 2 cut(s) 41, 731
HpyF10VI GCNNNNNNNGC 1 cut(s) 806
HpyF3I CTNAG 2 cut(s) 742, 849
HpySE526I ACGT 3 cut(s) 108, 450, 828
Hsp92I GRCGYC 1 cut(s) 828
Hsp92II CATG 5 cut(s) 63, 144, 395, 574, 731
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 3 cut(s) 56, 402, 693
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 2 cut(s) 130, 524
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 1 cut(s) 843
MaeI CTAG 2 cut(s) 542, 611
MaeII ACGT 3 cut(s) 108, 450, 828
MaeIII GTNAC 2 cut(s) 193, 626
MalI GATC 3 cut(s) 58, 404, 695
MbiI CCGCTC 1 cut(s) 86
MboI GATC 3 cut(s) 56, 402, 693
MboII GAAGA 4 cut(s) 457, 475, 479, 794
MfeI CAATTG 1 cut(s) 676
MflI RGATCY 1 cut(s) 402
MhlI GDGCHC 2 cut(s) 280, 317
MluCI AATT 5 cut(s) 44, 64, 78, 513, 676
MlyI GAGTC 2 cut(s) 25, 201
MmeI TCCRAC 1 cut(s) 380
MnlI CCTC 6 cut(s) 122, 369, 431, 448, 573, 878
Mph1103I ATGCAT 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 377
MslI CAYNNNNRTG 1 cut(s) 575
MspI CCGG 2 cut(s) 624, 657
MunI CAATTG 1 cut(s) 676
MwoI GCNNNNNNNGC 1 cut(s) 806
NdeII GATC 3 cut(s) 56, 402, 693
NlaIII CATG 5 cut(s) 63, 144, 395, 574, 731
NlaIV GGNNCC 2 cut(s) 8, 102
NmeAIII GCCGAG 1 cut(s) 599
NmuCI GTSAC 2 cut(s) 193, 626
NsbI TGCGCA 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 733
PciSI GCTCTTC 1 cut(s) 807
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 845
PflFI GACNNNGTC 1 cut(s) 753
PkrI GCNGC 4 cut(s) 88, 148, 163, 166
PleI GAGTC 2 cut(s) 25, 200
PpsI GAGTC 2 cut(s) 25, 200
Psp1406I AACGTT 1 cut(s) 450
PspN4I GGNNCC 2 cut(s) 8, 102
PspPI GGNCC 2 cut(s) 7, 100
PsuI RGATCY 1 cut(s) 402
PsyI GACNNNGTC 1 cut(s) 753
RsaI GTAC 2 cut(s) 257, 496
RsaNI GTAC 2 cut(s) 256, 495
RseI CAYNNNNRTG 1 cut(s) 575
SalI GTCGAC 1 cut(s) 773
SapI GCTCTTC 1 cut(s) 807
SatI GCNGC 4 cut(s) 87, 147, 162, 165
Sau3AI GATC 3 cut(s) 56, 402, 693
Sau96I GGNCC 2 cut(s) 7, 100
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 2 cut(s) 25, 201
SduI GDGCHC 2 cut(s) 280, 317
SfaNI GCATC 1 cut(s) 843
SfcI CTRYAG 1 cut(s) 478
SgrAI CRCCGGYG 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 251, 869
SmoI CTYRAG 2 cut(s) 251, 869
Sse9I AATT 5 cut(s) 44, 64, 78, 513, 676
SsiI CCGC 3 cut(s) 86, 162, 165
SspMI CTAG 2 cut(s) 542, 611
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 3 cut(s) 111, 453, 831
TaqI TCGA 4 cut(s) 421, 427, 748, 774
TasI AATT 5 cut(s) 44, 64, 78, 513, 676
TatI WGTACW 1 cut(s) 255
TauI GCSGC 3 cut(s) 89, 164, 167
TfiI GAWTC 1 cut(s) 845
TscAI CASTG 3 cut(s) 187, 476, 847
TseFI GTSAC 2 cut(s) 193, 626
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 2 cut(s) 193, 626
TspDTI ATGAA 2 cut(s) 476, 479
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 3 cut(s) 187, 476, 847
Tth111I GACNNNGTC 1 cut(s) 753
XapI RAATTY 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 541
XmiI GTMKAC 2 cut(s) 72, 774
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 2 cut(s) 542, 611
ZraI GACGTC 1 cut(s) 829
ZrmI AGTACT 1 cut(s) 257
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.