FvH4_4g27260

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001162
Physical Location & Seq
Forward (+)
28326847 .. 28329208
2362 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27260.t1

Sequence Viewer

Length: 876 bp
ATGCTGGCATCAGAACAGAGGAGGAATTCAAGGGGTATGGAAATTTTCTGCAAAAGCTGGTTGCCAAAACAAGGTGATCAGGTAAAAGGTGCCCTGTGTTTTTGCCATGGGTACGGTAGTACTTGTACATTCTTCTTTGAAGGCATTGCTAGGAGAATCACTCAATCCGGGTACGCTGTATATGCAGTAGACTATACAGGGTTTGGTCTTTCTGAAGGGTTGCATGGTTACATCTCAGACTTTGATGAGTTGGTCGATGATGTTATTGAACAATTTACTAGTATCAAAGGAAGATCTGAAGTGAAAGGTTTGCCGTTTTTTATAATGGGGGAGTCGATGGGTGGAGCTGTGACTCTAAAGATTCATCTAAAGGAACCAGATAAATGGGATGGAGTGATCCTTGTAGCTCCAATGTGTAAAATTGCAGATGATGTGATGCCTCCAGCTATAGTGCTGAAGCTATTAGCCTTCATGTCTGAAGTTTTGCCTCAGGCAAAACTCTTCCCACAGAAAGATATAGCTCATCTAAGCTACAGAGAACCCGCAAAGAGAAAAACGGCTGGTTACAATGTGATTTCTTACAAGGACCAAATGCGATCAAGAACAGCTGTAGAACTTCTGAAGGCGACTAGCGATATTGAAATGCACTTGGATAAGGTTTCATCCCCGTTGCTAATTCTTCATGGAGAAGCAGATAAGGTGACAGACCCTATAGTGAGCCAGTTGCTTTATGAAAAGGCCTCTAGCAAGGATAAGACTTTGAAGCTCTATCAAGATGCTTATCACTGCATTCTTCAAGGGGAGCCTGATGACACAATTTTCACCGTCCTTGATGACATTGTCACTTGGCTTGATTCCCGGTGCTTCCGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

292

Amino Acids

32.67

Weight (kDa)

5.84

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 27 - 269 9.3e-65 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 31 - 265 2e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 34 - 271 4e-13 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 189
AciI CCGC 1 cut(s) 543
AclWI GGATC 1 cut(s) 391
AcsI RAATTY 2 cut(s) 25, 42
AcuI CTGAAG 5 cut(s) 234, 318, 476, 498, 641
AfaI GTAC 4 cut(s) 113, 121, 127, 173
AfiI CCNNNNNNNGG 1 cut(s) 71
AgsI TTSAA 6 cut(s) 30, 140, 269, 641, 763, 797
AhlI ACTAGT 1 cut(s) 278
AluBI AGCT 9 cut(s) 57, 347, 407, 446, 460, 521, 531, 608, 766
AluI AGCT 9 cut(s) 57, 347, 407, 446, 460, 521, 531, 608, 766
AlwI GGATC 1 cut(s) 391
AoxI GGCC 1 cut(s) 738
ApoI RAATTY 2 cut(s) 25, 42
AspS9I GGNCC 1 cut(s) 586
AsuC2I CCSGG 2 cut(s) 169, 859
AsuHPI GGTGA 3 cut(s) 86, 712, 814
AvaII GGWCC 1 cut(s) 586
AxyI CCTNAGG 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 94
BaeI ACNNNNGTAYC 2 cut(s) 103, 136
BanI GGYRCC 1 cut(s) 89
BccI CCATC 2 cut(s) 331, 383
BceAI ACGGC 2 cut(s) 298, 573
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 2 cut(s) 169, 859
BcuI ACTAGT 1 cut(s) 278
BfaI CTAG 4 cut(s) 150, 279, 630, 744
BfmI CTRYAG 4 cut(s) 447, 532, 609, 711
BglII AGATCT 1 cut(s) 293
BmcAI AGTACT 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 169, 859
Bme18I GGWCC 1 cut(s) 586
BmgT120I GGNCC 1 cut(s) 586
BmiI GGNNCC 3 cut(s) 91, 375, 804
BmrFI CCNGG 2 cut(s) 169, 859
BmsI GCATC 3 cut(s) 17, 426, 766
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpmI CTGGAG 1 cut(s) 426
BpuMI CCSGG 2 cut(s) 169, 859
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 721
Bse21I CCTNAGG 1 cut(s) 489
Bse3DI GCAATG 1 cut(s) 144
Bse8I GATNNNNATC 1 cut(s) 780
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 2 cut(s) 394, 662
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 2 cut(s) 249, 503
BseNI ACTGG 1 cut(s) 721
BseRI GAGGAG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 94
BshFI GGCC 1 cut(s) 740
BshNI GGYRCC 1 cut(s) 89
BsiSI CCGG 2 cut(s) 168, 859
BslI CCNNNNNNNGG 1 cut(s) 71
BsmI GAATGC 1 cut(s) 789
BsnI GGCC 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 125
Bsp143I GATC 4 cut(s) 76, 293, 396, 596
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 1 cut(s) 543
BspANI GGCC 1 cut(s) 740
BspCNI CTCAG 2 cut(s) 248, 502
BspLI GGNNCC 3 cut(s) 91, 375, 804
BspPI GGATC 1 cut(s) 391
BspT107I GGYRCC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 125
BsrI ACTGG 1 cut(s) 721
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 4 cut(s) 76, 293, 396, 596
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 2 cut(s) 116, 826
Bst6I CTCTTC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 235, 489, 527
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 2 cut(s) 394, 662
BstKTI GATC 4 cut(s) 79, 296, 399, 599
BstMBI GATC 4 cut(s) 76, 293, 396, 596
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 167, 857
BstSFI CTRYAG 4 cut(s) 447, 532, 609, 711
BstSLI GKGCMC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 293
BstXI CCANNNNNNTGG 1 cut(s) 384
BstYI RGATCY 1 cut(s) 293
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 1 cut(s) 740
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 2 cut(s) 394, 662
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 1 cut(s) 784
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 586
Csp6I GTAC 4 cut(s) 112, 120, 126, 172
CviAII CATG 4 cut(s) 107, 224, 472, 683
CviQI GTAC 4 cut(s) 112, 120, 126, 172
DdeI CTNAG 3 cut(s) 235, 489, 527
DpnI GATC 4 cut(s) 78, 295, 398, 598
DpnII GATC 4 cut(s) 76, 293, 396, 596
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 740
Eco47I GGWCC 1 cut(s) 586
Eco57I CTGAAG 5 cut(s) 234, 318, 476, 498, 641
Eco81I CCTNAGG 1 cut(s) 489
EcoRI GAATTC 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 4 cut(s) 110, 227, 475, 686
FatI CATG 4 cut(s) 106, 223, 471, 682
FauI CCCGC 1 cut(s) 550
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 1 cut(s) 189
FokI GGATG 2 cut(s) 401, 649
FspBI CTAG 4 cut(s) 150, 279, 630, 744
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 1 cut(s) 740
HapII CCGG 2 cut(s) 168, 859
Hin1II CATG 4 cut(s) 110, 227, 475, 686
HinfI GANTC 6 cut(s) 156, 332, 352, 361, 854, 870
HpaII CCGG 2 cut(s) 168, 859
HphI GGTGA 3 cut(s) 86, 712, 814
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 7 cut(s) 13, 214, 238, 298, 478, 621, 869
Hpy188III TCNNGA 2 cut(s) 600, 773
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 4 cut(s) 134, 209, 478, 616
HpyCH4III ACNGT 2 cut(s) 116, 826
HpyCH4V TGCA 6 cut(s) 51, 185, 223, 425, 646, 789
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 3 cut(s) 235, 489, 527
Hsp92II CATG 4 cut(s) 110, 227, 475, 686
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 4 cut(s) 76, 293, 396, 596
LmnI GCTCC 3 cut(s) 344, 412, 802
LweI GCATC 3 cut(s) 17, 426, 766
MaeI CTAG 4 cut(s) 150, 279, 630, 744
MaeIII GTNAC 5 cut(s) 227, 349, 563, 700, 841
MalI GATC 4 cut(s) 78, 295, 398, 598
MboI GATC 4 cut(s) 76, 293, 396, 596
MboII GAAGA 5 cut(s) 124, 303, 493, 671, 785
MflI RGATCY 1 cut(s) 293
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 6 cut(s) 25, 42, 272, 420, 675, 816
MlyI GAGTC 2 cut(s) 341, 346
MnlI CCTC 5 cut(s) 12, 15, 450, 498, 751
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 2 cut(s) 168, 859
MspR9I CCNGG 2 cut(s) 169, 859
Mva1269I GAATGC 1 cut(s) 789
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 2 cut(s) 169, 859
NcoI CCATGG 1 cut(s) 106
NdeII GATC 4 cut(s) 76, 293, 396, 596
NlaIII CATG 4 cut(s) 110, 227, 475, 686
NlaIV GGNNCC 3 cut(s) 91, 375, 804
NmuCI GTSAC 3 cut(s) 349, 700, 841
PceI AGGCCT 1 cut(s) 740
PctI GAATGC 1 cut(s) 789
PfeI GAWTC 4 cut(s) 156, 361, 854, 870
PflFI GACNNNGTC 1 cut(s) 839
PleI GAGTC 2 cut(s) 340, 346
PpsI GAGTC 2 cut(s) 340, 346
PsiI TTATAA 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 91, 375, 804
PspPI GGNCC 1 cut(s) 586
PsuI RGATCY 1 cut(s) 293
PsyI GACNNNGTC 1 cut(s) 839
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 4 cut(s) 113, 121, 127, 173
RsaNI GTAC 4 cut(s) 112, 120, 126, 172
Sau3AI GATC 4 cut(s) 76, 293, 396, 596
Sau96I GGNCC 1 cut(s) 586
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 2 cut(s) 341, 346
ScrFI CCNGG 2 cut(s) 169, 859
SduI GDGCHC 1 cut(s) 94
SfaNI GCATC 3 cut(s) 17, 426, 766
SfcI CTRYAG 4 cut(s) 447, 532, 609, 711
SinI GGWCC 1 cut(s) 586
SpeI ACTAGT 1 cut(s) 278
Sse9I AATT 6 cut(s) 25, 42, 272, 420, 675, 816
SseBI AGGCCT 1 cut(s) 740
SsiI CCGC 1 cut(s) 543
SspMI CTAG 4 cut(s) 150, 279, 630, 744
StuI AGGCCT 1 cut(s) 740
StyD4I CCNGG 2 cut(s) 167, 857
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 2 cut(s) 116, 826
TaqI TCGA 2 cut(s) 255, 335
TasI AATT 6 cut(s) 25, 42, 272, 420, 675, 816
TatI WGTACW 2 cut(s) 119, 125
TfiI GAWTC 4 cut(s) 156, 361, 854, 870
TscAI CASTG 1 cut(s) 791
TseFI GTSAC 3 cut(s) 349, 700, 841
Tsp45I GTSAC 3 cut(s) 349, 700, 841
TspDTI ATGAA 5 cut(s) 353, 460, 651, 671, 747
TspRI CASTG 1 cut(s) 791
Tth111I GACNNNGTC 1 cut(s) 839
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 2 cut(s) 25, 42
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 4 cut(s) 150, 279, 630, 744
ZrmI AGTACT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.