pycom16g03620

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001162
Physical Location & Seq
Forward (+)
2242598 .. 2245586
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03620.8

Sequence Viewer

Length: 1341 bp
ATGCCACCTCAACAGGATAAATCGATAATTAAGGCGATTATAAATCTACACGTTTTCTTTGAGTTTACCAAAAAGCAAAGAACTTTTGAACATCAAACGTTGAATCCACTTGACTTTCCAAAAGCCCGTCCAATCTTTACAACCATGATCCTGTCACCTACTCCGACTTTCCGACCACGAACCCCTCCTCCGTTTCACCGGAAATCAAGCTCTCCGAGCAAACATCCACAAAAAAGGCACCTACCCTCTTTCCTCCCATCAAAATCGTATCCGAAAACCCCAAAAACTCACTTCAGAGTTATGGCCGGGAAACGGTTGCCAATGGAAGGGTTAAGTCGAGAGATGTGCGCCATAGCTTCGCAGAATCTGGACCATGCGCCGGCTCGAAGACGAGTCCGGTCCGCATTCATCGACGTGCATCAGCAACTTGATCATTGCCTGTTCAAGATGGATCATAGTGGCATCAGAACAGAGGAGTGGTATGGAAGGAATTCAAGGGGTATGGAGATTTTCTGCAAAAGCTGGTTGCCAAAAGCTGGTGATCGAATCAAAGGTGCTTTATGCTTCTGCCATGGCTATGGTAGTACTTGCACATTTTTCTTTGAAGGTATCGCCAAGCGAATTGCTGCATCTGGATACGCTATATACGCCGTGGACTATCCGGGTTTCGGTCTTTCTGAAGGATTGCATGGTTACATCCCAAACTTTGATGAATTAGTCGGCGATGTAATCGAACAGTTTACTAATGTCAAAGGAAGATCTGATGTGAAAGGTCTGCCATTCTTTGTAATGGGAGAATCCATGGGAGGAGCCGTGAGTCTCAAGATTCATCTTAAGGAGCCGGACAAATGGGATGGTGTAATCCTTGTAGCTCCAATGTGCAAAATAGCGGAAGACGTGATGCCTCCGGCAATGGTACTGAAGCTATTAGCCCTGATGTCTGAAGTTTTGCCTGAGGCGAAACTCTTCCCGCAGAAAGATACATCCGAGCTAAGCTACAGAGATCCGAAGAACAGAAAAACGGCCGGTTACAATGTGATTTCTTACAACGACAACATGCGATCAAGAACAGCTGTGGAACTTCTGAAGGCCACAAGTGATATCGAAACGCAGTTGCATAAGGTTTCGGGCCCGGTTCTAATTCTTCATGGAGCAGAAGATAAGGTGACAGATCCTTTGGTGAGCCGGTTGCTTTACGAGAAGGCATCTGGCAAGGATAAAACTTTGAAGCTCTATCCGGATGGTTTTCACTGCATTCTTCAAGGGGAGCCCGATGACAGAATTTTCACTGTTCTTGATGACATTGTAACATGGCTTGATTCCCGTTGCTCCCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

447

Amino Acids

50.34

Weight (kDa)

9.14

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 536
AccIII TCCGGA 1 cut(s) 1237
AciI CCGC 3 cut(s) 402, 890, 971
AclI AACGTT 1 cut(s) 98
AclWI GGATC 4 cut(s) 142, 459, 998, 1166
AcoI YGGCCR 2 cut(s) 303, 1023
AcsI RAATTY 2 cut(s) 490, 1281
AcuI CTGAAG 5 cut(s) 277, 699, 941, 963, 1106
AfaI GTAC 2 cut(s) 586, 918
AfiI CCNNNNNNNGG 5 cut(s) 312, 326, 379, 536, 668
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 7 cut(s) 89, 103, 445, 495, 605, 1228, 1262
AjiI CACGTC 2 cut(s) 415, 898
AloI GAACNNNNNNTCC 2 cut(s) 172, 204
Alw26I GTCTC 1 cut(s) 824
AlwI GGATC 4 cut(s) 142, 459, 998, 1166
AlwNI CAGNNNCTG 1 cut(s) 367
Aor13HI TCCGGA 1 cut(s) 1237
AoxI GGCC 4 cut(s) 303, 1023, 1089, 1129
ApaI GGGCCC 1 cut(s) 1133
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 2 cut(s) 490, 1281
ArsI GACNNNNNNTTYG 2 cut(s) 1159, 1191
Asp700I GAANNNNTTC 2 cut(s) 490, 965
AspLEI GCGC 2 cut(s) 350, 379
AspS9I GGNCC 4 cut(s) 370, 399, 1129, 1130
AsuC2I CCSGG 3 cut(s) 307, 663, 1133
AsuHPI GGTGA 5 cut(s) 147, 188, 551, 1177, 1192
AvaII GGWCC 2 cut(s) 370, 399
AxyI CCTNAGG 1 cut(s) 954
BaeGI GKGCMC 1 cut(s) 1133
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 2 cut(s) 1133, 1272
BbsI GAAGAC 2 cut(s) 394, 900
BbvI GCAGC 1 cut(s) 613
BccI CCATC 4 cut(s) 265, 442, 848, 1235
BceAI ACGGC 3 cut(s) 635, 797, 1038
BcgI CGANNNNNNTGC 2 cut(s) 327, 361
BciVI GTATCC 2 cut(s) 279, 629
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 3 cut(s) 307, 663, 1133
BcoDI GTCTC 1 cut(s) 824
BfmI CTRYAG 1 cut(s) 997
BfrI CTTAAG 1 cut(s) 833
BfuI GTATCC 2 cut(s) 279, 629
BglII AGATCT 1 cut(s) 758
BisI GCNGC 1 cut(s) 627
BlpI GCTNAGC 1 cut(s) 992
BlsI GCNGC 1 cut(s) 628
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 3 cut(s) 307, 663, 1133
Bme18I GGWCC 2 cut(s) 370, 399
BmgBI CACGTC 2 cut(s) 415, 898
BmgT120I GGNCC 4 cut(s) 370, 399, 1129, 1130
BmiI GGNNCC 5 cut(s) 239, 811, 840, 1131, 1269
BmrFI CCNGG 3 cut(s) 307, 663, 1133
BmsI GCATC 5 cut(s) 427, 471, 638, 891, 1214
BpiI GAAGAC 2 cut(s) 394, 900
Bpu1102I GCTNAGC 1 cut(s) 992
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 3 cut(s) 307, 663, 1133
Bsa29I ATCGAT 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 571, 651, 801
BsaWI WCCGGW 3 cut(s) 198, 396, 1237
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 798, 828
Bsc4I CCNNNNNNNGG 5 cut(s) 312, 326, 379, 536, 668
Bse118I RCCGGY 3 cut(s) 379, 1025, 1185
Bse21I CCTNAGG 1 cut(s) 954
Bse3DI GCAATG 2 cut(s) 433, 918
BseAI TCCGGA 1 cut(s) 1237
BseCI ATCGAT 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 571, 651, 801
BseGI GGATG 5 cut(s) 223, 696, 859, 983, 1246
BseLI CCNNNNNNNGG 5 cut(s) 312, 326, 379, 536, 668
BseMI GCAATG 2 cut(s) 433, 918
BseMII CTCAG 1 cut(s) 945
BseRI GAGGAG 3 cut(s) 177, 488, 822
BseSI GKGCMC 1 cut(s) 1133
BseX3I CGGCCG 1 cut(s) 1023
BseXI GCAGC 1 cut(s) 613
Bsh1285I CGRYCG 1 cut(s) 1026
BshFI GGCC 4 cut(s) 305, 1025, 1091, 1131
BshNI GGYRCC 1 cut(s) 237
BshVI ATCGAT 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 1026
BslI CCNNNNNNNGG 5 cut(s) 312, 326, 379, 536, 668
BsmAI GTCTC 1 cut(s) 824
BsmI GAATGC 2 cut(s) 404, 1254
BsnI GGCC 4 cut(s) 305, 1025, 1091, 1131
Bsp120I GGGCCC 1 cut(s) 1129
Bsp1286I GDGCHC 2 cut(s) 1133, 1272
Bsp13I TCCGGA 1 cut(s) 1237
Bsp143I GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
Bsp1720I GCTNAGC 1 cut(s) 992
Bsp19I CCATGG 2 cut(s) 571, 801
BspACI CCGC 3 cut(s) 402, 890, 971
BspANI GGCC 4 cut(s) 305, 1025, 1091, 1131
BspCNI CTCAG 1 cut(s) 946
BspDI ATCGAT 1 cut(s) 23
BspEI TCCGGA 1 cut(s) 1237
BspLI GGNNCC 5 cut(s) 239, 811, 840, 1131, 1269
BspPI GGATC 4 cut(s) 142, 459, 998, 1166
BspT107I GGYRCC 1 cut(s) 237
BspTI CTTAAG 1 cut(s) 833
BsrDI GCAATG 2 cut(s) 433, 918
BsrFI RCCGGY 3 cut(s) 379, 1025, 1185
BssAI RCCGGY 3 cut(s) 379, 1025, 1185
BssECI CCNNGG 3 cut(s) 571, 651, 801
BssMI GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
BssT1I CCWWGG 2 cut(s) 571, 801
Bst4CI ACNGT 3 cut(s) 315, 738, 1291
Bst6I CTCTTC 1 cut(s) 971
BstAFI CTTAAG 1 cut(s) 833
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 2 cut(s) 954, 992
BstDSI CCRYGG 3 cut(s) 571, 651, 801
BstF5I GGATG 5 cut(s) 223, 696, 859, 983, 1246
BstHHI GCGC 2 cut(s) 350, 379
BstKTI GATC 8 cut(s) 150, 433, 454, 544, 761, 1006, 1064, 1174
BstMAI GTCTC 1 cut(s) 824
BstMBI GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
BstMCI CGRYCG 1 cut(s) 1026
BstMWI GCNNNNNNNGC 2 cut(s) 216, 647
BstNSI RCATGY 1 cut(s) 1060
BstSCI CCNGG 3 cut(s) 305, 661, 1131
BstSFI CTRYAG 1 cut(s) 997
BstSLI GKGCMC 1 cut(s) 1133
BstV1I GCAGC 1 cut(s) 613
BstV2I GAAGAC 2 cut(s) 394, 900
BstX2I RGATCY 3 cut(s) 758, 1003, 1171
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 3 cut(s) 758, 1003, 1171
BstZI CGGCCG 1 cut(s) 1023
Bsu15I ATCGAT 1 cut(s) 23
Bsu36I CCTNAGG 1 cut(s) 954
BsuI GTATCC 2 cut(s) 279, 629
BsuRI GGCC 4 cut(s) 305, 1025, 1091, 1131
BsuTUI ATCGAT 1 cut(s) 23
BtgI CCRYGG 3 cut(s) 571, 651, 801
BtgZI GCGATG 1 cut(s) 738
BtrI CACGTC 2 cut(s) 415, 898
BtsCI GGATG 5 cut(s) 223, 696, 859, 983, 1246
BtsI GCAGTG 1 cut(s) 1249
BtsIMutI CAGTG 2 cut(s) 1249, 1287
Cac8I GCNNGC 1 cut(s) 381
CaiI CAGNNNCTG 1 cut(s) 367
CfoI GCGC 2 cut(s) 350, 379
Cfr10I RCCGGY 3 cut(s) 379, 1025, 1185
Cfr13I GGNCC 4 cut(s) 370, 399, 1129, 1130
ClaI ATCGAT 1 cut(s) 23
CpoI CGGWCCG 1 cut(s) 399
Csp6I GTAC 2 cut(s) 585, 917
CspI CGGWCCG 1 cut(s) 399
CviAII CATG 8 cut(s) 145, 374, 572, 689, 802, 1057, 1148, 1311
CviQI GTAC 2 cut(s) 585, 917
DdeI CTNAG 2 cut(s) 954, 992
DpnI GATC 8 cut(s) 149, 432, 453, 543, 760, 1005, 1063, 1173
DpnII GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
EaeI YGGCCR 2 cut(s) 303, 1023
EagI CGGCCG 1 cut(s) 1023
Eam1104I CTCTTC 1 cut(s) 971
EarI CTCTTC 1 cut(s) 971
EclXI CGGCCG 1 cut(s) 1023
Eco130I CCWWGG 2 cut(s) 571, 801
Eco24I GRGCYC 2 cut(s) 1133, 1272
Eco32I GATATC 1 cut(s) 1102
Eco47I GGWCC 2 cut(s) 370, 399
Eco52I CGGCCG 1 cut(s) 1023
Eco57I CTGAAG 5 cut(s) 277, 699, 941, 963, 1106
Eco81I CCTNAGG 1 cut(s) 954
EcoRI GAATTC 1 cut(s) 490
EcoRV GATATC 1 cut(s) 1102
EcoT14I CCWWGG 2 cut(s) 571, 801
EcoT38I GRGCYC 2 cut(s) 1133, 1272
ErhI CCWWGG 2 cut(s) 571, 801
FaeI CATG 8 cut(s) 148, 377, 575, 692, 805, 1060, 1151, 1314
FatI CATG 8 cut(s) 144, 373, 571, 688, 801, 1056, 1147, 1310
FauI CCCGC 1 cut(s) 978
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 5 cut(s) 210, 683, 866, 970, 1253
FriOI GRGCYC 2 cut(s) 1133, 1272
Fsp4HI GCNGC 1 cut(s) 627
GlaI GCGC 2 cut(s) 349, 378
GluI GCNGC 1 cut(s) 627
HaeIII GGCC 4 cut(s) 305, 1025, 1091, 1131
HhaI GCGC 2 cut(s) 350, 379
Hin1II CATG 8 cut(s) 148, 377, 575, 692, 805, 1060, 1151, 1314
Hin6I GCGC 2 cut(s) 348, 377
HinP1I GCGC 2 cut(s) 348, 377
HinfI GANTC 8 cut(s) 103, 364, 393, 546, 797, 817, 826, 1319
HphI GGTGA 5 cut(s) 147, 188, 551, 1177, 1192
Hpy166II GTNNAC 3 cut(s) 66, 655, 741
Hpy188III TCNNGA 8 cut(s) 338, 368, 445, 633, 823, 1065, 1238, 1295
Hpy8I GTNNAC 3 cut(s) 66, 655, 741
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 6 cut(s) 320, 480, 599, 674, 1081, 1195
HpyCH4III ACNGT 3 cut(s) 315, 738, 1291
HpyCH4IV ACGT 4 cut(s) 51, 98, 414, 897
HpyCH4V TGCA 8 cut(s) 418, 516, 591, 629, 688, 882, 1117, 1254
HpyF10VI GCNNNNNNNGC 2 cut(s) 216, 647
HpyF3I CTNAG 2 cut(s) 954, 992
HpySE526I ACGT 4 cut(s) 51, 98, 414, 897
Hsp92II CATG 8 cut(s) 148, 377, 575, 692, 805, 1060, 1151, 1314
HspAI GCGC 2 cut(s) 348, 377
Kpn2I TCCGGA 1 cut(s) 1237
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
LmnI GCTCC 6 cut(s) 809, 838, 877, 1151, 1267, 1334
Lsp1109I GCAGC 1 cut(s) 613
LweI GCATC 5 cut(s) 427, 471, 638, 891, 1214
MaeII ACGT 4 cut(s) 51, 98, 414, 897
MaeIII GTNAC 5 cut(s) 153, 692, 1028, 1165, 1306
MalI GATC 8 cut(s) 149, 432, 453, 543, 760, 1005, 1063, 1173
MboI GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
MboII GAAGA 8 cut(s) 399, 768, 905, 958, 1021, 1136, 1169, 1250
MflI RGATCY 3 cut(s) 758, 1003, 1171
MhlI GDGCHC 2 cut(s) 1133, 1272
MluCI AATT 7 cut(s) 27, 490, 621, 713, 1140, 1281, 1336
MlyI GAGTC 2 cut(s) 402, 826
MmeI TCCRAC 2 cut(s) 188, 196
MnlI CCTC 9 cut(s) 18, 195, 198, 256, 263, 466, 800, 915, 949
MroI TCCGGA 1 cut(s) 1237
MroNI GCCGGC 1 cut(s) 379
MroXI GAANNNNTTC 2 cut(s) 490, 965
MseI TTAA 4 cut(s) 30, 332, 834, 1339
MslI CAYNNNNRTG 2 cut(s) 413, 576
MspA1I CMGCKG 1 cut(s) 1073
MspCI CTTAAG 1 cut(s) 833
MspR9I CCNGG 3 cut(s) 307, 663, 1133
Mva1269I GAATGC 2 cut(s) 404, 1254
MwoI GCNNNNNNNGC 2 cut(s) 216, 647
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 3 cut(s) 307, 663, 1133
NcoI CCATGG 2 cut(s) 571, 801
NdeII GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 8 cut(s) 148, 377, 575, 692, 805, 1060, 1151, 1314
NlaIV GGNNCC 5 cut(s) 239, 811, 840, 1131, 1269
NmuCI GTSAC 2 cut(s) 153, 1165
NspI RCATGY 1 cut(s) 1060
PctI GAATGC 2 cut(s) 404, 1254
PdiI GCCGGC 1 cut(s) 381
PdmI GAANNNNTTC 2 cut(s) 490, 965
PfeI GAWTC 6 cut(s) 103, 364, 546, 797, 826, 1319
PflMI CCANNNNNTGG 1 cut(s) 536
PkrI GCNGC 1 cut(s) 628
PleI GAGTC 2 cut(s) 401, 825
PpsI GAGTC 2 cut(s) 401, 825
PsiI TTATAA 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 98
PspN4I GGNNCC 5 cut(s) 239, 811, 840, 1131, 1269
PspOMI GGGCCC 1 cut(s) 1129
PspPI GGNCC 4 cut(s) 370, 399, 1129, 1130
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 3 cut(s) 758, 1003, 1171
PvuII CAGCTG 1 cut(s) 1073
RsaI GTAC 2 cut(s) 586, 918
RsaNI GTAC 2 cut(s) 585, 917
RseI CAYNNNNRTG 2 cut(s) 413, 576
Rsr2I CGGWCCG 1 cut(s) 399
RsrII CGGWCCG 1 cut(s) 399
SaqAI TTAA 4 cut(s) 30, 332, 834, 1339
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 8 cut(s) 147, 430, 451, 541, 758, 1003, 1061, 1171
Sau96I GGNCC 4 cut(s) 370, 399, 1129, 1130
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 2 cut(s) 402, 826
ScrFI CCNGG 3 cut(s) 307, 663, 1133
SduI GDGCHC 2 cut(s) 1133, 1272
SfaNI GCATC 5 cut(s) 427, 471, 638, 891, 1214
SfcI CTRYAG 1 cut(s) 997
SinI GGWCC 2 cut(s) 370, 399
SmiMI CAYNNNNRTG 2 cut(s) 413, 576
SmlI CTYRAG 2 cut(s) 821, 833
SmoI CTYRAG 2 cut(s) 821, 833
Sse9I AATT 7 cut(s) 27, 490, 621, 713, 1140, 1281, 1336
SsiI CCGC 3 cut(s) 402, 890, 971
StyD4I CCNGG 3 cut(s) 305, 661, 1131
StyI CCWWGG 2 cut(s) 571, 801
TaaI ACNGT 3 cut(s) 315, 738, 1291
TaiI ACGT 4 cut(s) 54, 101, 417, 900
TaqI TCGA 7 cut(s) 23, 337, 385, 411, 544, 732, 1104
TaqII GACCGA 1 cut(s) 659
TasI AATT 7 cut(s) 27, 490, 621, 713, 1140, 1281, 1336
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 6 cut(s) 103, 364, 546, 797, 826, 1319
Tru1I TTAA 4 cut(s) 30, 332, 834, 1339
Tru9I TTAA 4 cut(s) 30, 332, 834, 1339
TscAI CASTG 2 cut(s) 1256, 1294
TseFI GTSAC 2 cut(s) 153, 1165
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 2 cut(s) 153, 1165
TspDTI ATGAA 4 cut(s) 397, 726, 818, 1136
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 2 cut(s) 1256, 1294
Van91I CCANNNNNTGG 1 cut(s) 536
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 370, 399
XapI RAATTY 2 cut(s) 490, 1281
XceI RCATGY 1 cut(s) 1060
XmnI GAANNNNTTC 2 cut(s) 490, 965
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.