Prupe.1G312100_v2.0.a1

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001162
Physical Location & Seq
Reverse (-)
30287567 .. 30291123
3557 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G312100.2

Sequence Viewer

Length: 1182 bp
ATGGACCTTTCCCGTGCTCTGAGATACCAACCTCTTAAGCTTCCTTTGTTTCCTCCTCAATTCCAAAACCCAATCAACCATCTTCATCCAAAGCCCACATTTTTAGTCCCCAGAGGAACTCGCCTAATGGTTACCGCCAAGAAAAGGCCACCCATTGAAGGTGTAAGTGAGGAGCTGAACTCGATCGCTAACCAGAACCTCGACTTTGCTTATACTCGCAGGCAGGTTCGATCCGCTTTCGTTGAGGTCCAGCAACAGCTTGATCATTGCTTGTTTAAGTTGGCTCCTACTGGGATCAGAACAGAGGAGTGGTACGAAAGAAATTCGAGGGGGTTGCAAATTTTCTGTAAAAGCTGGATGCCAAAACAAGGTGTTCCAATTAAAGGTGTTTTGTTTTTTTGTCATGGGTATGGTGACACTTGCGTTTTCTTCTTTGAAGGTATAGCCAGGCGAATTGCTGCATCTGGGTACGCTGTATATGCCATGGACTATCCAGGTTTTGGTCTTTCTGAAGGATTGCATGGTTACATTCCAAGCTTTGATCAATTAGCTGATGATGTGATTGAACAGTACACAAAGATTAAAGGAAGACCAGAGTTGAAAGGGTTGCCCCACTTTATATTGGGGCAGTCCATGGGTGGAGCCGTTACTCTCAAAGTTCACTTAAAGGAACCATATGCATGGGATGGAGTTGTTCTCGTGGCCCCAATGTGTAAAATTGCAGAGGATGTAACGCCTCCAGCAGCTGCTCAGAAGATATTAATTCTTATGTCAAAAGTTATGCCGAAAGCAAAGCTTATCCCACAGAAAGATTTAGCTGAGCTGGCTTTCAGAGATCCGATAAAAAGGAAAATGGCTGTTTACAATGTGACATGCTACAATGATCACGTGCGGTTGAAGACTGCAGTGGAACTTCTATATGCTACCAAGGATATTGAGATGCAAGTTGAGAAGATATGTCAGGTTTCATCTCCACTGCTAATTCTTCATGGAGCAGCAGATAAGGTGACTGATCCCCTAGTGAGTCAGTATCTTTATGAGAAGGCCTCTAGCGAGGATAAAACCCTTAAACTCTACCCGGAAGGTTATCATTGCATTCTTGAAGGAGAACCTGATGACAGAATTTTTTCAGTCCTTGATGATATTATCACTTGGCTTGATTCCCGGTGTGCCCTGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

394

Amino Acids

44.44

Weight (kDa)

8.24

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 500
AciI CCGC 3 cut(s) 135, 234, 892
AclWI GGATC 4 cut(s) 225, 302, 830, 1007
AcsI RAATTY 3 cut(s) 322, 339, 1122
AcuI CTGAAG 1 cut(s) 531
AcvI CACGTG 1 cut(s) 889
AfaI GTAC 3 cut(s) 314, 470, 572
AfiI CCNNNNNNNGG 5 cut(s) 144, 158, 368, 383, 500
AflII CTTAAG 1 cut(s) 35
AgsI TTSAA 6 cut(s) 158, 437, 566, 601, 898, 1103
AjnI CCWGG 2 cut(s) 446, 493
Alw21I GWGCWC 1 cut(s) 19
AlwI GGATC 4 cut(s) 225, 302, 830, 1007
AlwNI CAGNNNCTG 1 cut(s) 746
AoxI GGCC 3 cut(s) 146, 702, 1044
ApeKI GCWGC 4 cut(s) 458, 743, 746, 995
ApoI RAATTY 3 cut(s) 322, 339, 1122
AseI ATTAAT 1 cut(s) 761
Asp700I GAANNNNTTC 1 cut(s) 1126
AspS9I GGNCC 3 cut(s) 4, 247, 703
AsuC2I CCSGG 2 cut(s) 1079, 1165
AsuHPI GGTGA 2 cut(s) 425, 1018
AvaII GGWCC 2 cut(s) 4, 247
BaeGI GKGCMC 1 cut(s) 1174
BauI CACGAG 1 cut(s) 698
BbrPI CACGTG 1 cut(s) 889
BbsI GAAGAC 2 cut(s) 595, 905
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 4 cut(s) 445, 733, 755, 1007
BccI CCATC 2 cut(s) 87, 680
BceAI ACGGC 1 cut(s) 629
BcgI CGANNNNNNTGC 2 cut(s) 316, 350
BciT130I CCWGG 2 cut(s) 448, 495
BclI TGATCA 3 cut(s) 262, 541, 883
BcnI CCSGG 2 cut(s) 1079, 1165
BfaI CTAG 2 cut(s) 1019, 1050
BfmI CTRYAG 1 cut(s) 903
BfrI CTTAAG 1 cut(s) 35
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 4 cut(s) 459, 744, 747, 996
BlpI GCTNAGC 1 cut(s) 819
BlsI GCNGC 4 cut(s) 460, 745, 748, 997
Bme1390I CCNGG 4 cut(s) 448, 495, 1079, 1165
Bme18I GGWCC 2 cut(s) 4, 247
BmgT120I GGNCC 3 cut(s) 4, 247, 703
BmiI GGNNCC 4 cut(s) 285, 643, 672, 705
BmrFI CCNGG 4 cut(s) 448, 495, 1079, 1165
BmrI ACTGGG 1 cut(s) 300
BmsI GCATC 3 cut(s) 348, 470, 930
BmuI ACTGGG 1 cut(s) 300
BpiI GAAGAC 2 cut(s) 595, 905
BplI GAGNNNNNCTC 6 cut(s) 164, 196, 681, 713, 1031, 1063
BpmI CTGGAG 1 cut(s) 723
Bpu1102I GCTNAGC 1 cut(s) 819
BpuMI CCSGG 2 cut(s) 1079, 1165
BsaAI YACGTR 1 cut(s) 889
BsaJI CCNNGG 3 cut(s) 483, 633, 927
Bsc4I CCNNNNNNNGG 5 cut(s) 144, 158, 368, 383, 500
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 2 cut(s) 265, 1090
BseBI CCWGG 2 cut(s) 448, 495
BseDI CCNNGG 3 cut(s) 483, 633, 927
BseGI GGATG 4 cut(s) 85, 363, 691, 733
BseLI CCNNNNNNNGG 5 cut(s) 144, 158, 368, 383, 500
BseMI GCAATG 2 cut(s) 265, 1090
BseMII CTCAG 3 cut(s) 11, 764, 810
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 3 cut(s) 45, 185, 320
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 4 cut(s) 445, 733, 755, 1007
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 148, 704, 1046
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 2 cut(s) 1079, 1165
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 5 cut(s) 144, 158, 368, 383, 500
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 1095
BsnI GGCC 3 cut(s) 148, 704, 1046
Bsp1286I GDGCHC 2 cut(s) 19, 1174
Bsp143I GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
Bsp1720I GCTNAGC 1 cut(s) 819
Bsp19I CCATGG 2 cut(s) 483, 633
BspACI CCGC 3 cut(s) 135, 234, 892
BspANI GGCC 3 cut(s) 148, 704, 1046
BspCNI CTCAG 3 cut(s) 12, 763, 811
BspLI GGNNCC 4 cut(s) 285, 643, 672, 705
BspMAI CTGCAG 1 cut(s) 907
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 4 cut(s) 225, 302, 830, 1007
BspTI CTTAAG 1 cut(s) 35
BsrDI GCAATG 2 cut(s) 265, 1090
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 483, 633, 927
BssMI GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
BssSI CACGAG 1 cut(s) 698
BssT1I CCWWGG 3 cut(s) 483, 633, 927
Bst2BI CACGAG 1 cut(s) 698
Bst2UI CCWGG 2 cut(s) 448, 495
Bst4CI ACNGT 1 cut(s) 570
BstAFI CTTAAG 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 889
BstC8I GCNNGC 2 cut(s) 221, 825
BstDEI CTNAG 3 cut(s) 20, 750, 819
BstDSI CCRYGG 2 cut(s) 483, 633
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 4 cut(s) 85, 363, 691, 733
BstKTI GATC 8 cut(s) 186, 233, 265, 297, 544, 838, 886, 1015
BstMBI GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 479, 824
BstNI CCWGG 2 cut(s) 448, 495
BstNSI RCATGY 1 cut(s) 876
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 446, 493, 1077, 1163
BstSFI CTRYAG 1 cut(s) 903
BstSLI GKGCMC 1 cut(s) 1174
BstV1I GCAGC 4 cut(s) 445, 733, 755, 1007
BstV2I GAAGAC 2 cut(s) 595, 905
BstX2I RGATCY 1 cut(s) 835
BstXI CCANNNNNNTGG 1 cut(s) 681
BstYI RGATCY 1 cut(s) 835
BsuRI GGCC 3 cut(s) 148, 704, 1046
BtgI CCRYGG 2 cut(s) 483, 633
BtsCI GGATG 4 cut(s) 85, 363, 691, 733
BtsI GCAGTG 2 cut(s) 912, 974
BtsIMutI CAGTG 2 cut(s) 912, 974
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 221, 825
CaiI CAGNNNCTG 1 cut(s) 746
Cfr13I GGNCC 3 cut(s) 4, 247, 703
Csp6I GTAC 3 cut(s) 313, 469, 571
CviAII CATG 7 cut(s) 404, 484, 521, 634, 681, 873, 989
CviQI GTAC 3 cut(s) 313, 469, 571
DdeI CTNAG 3 cut(s) 20, 750, 819
DpnI GATC 8 cut(s) 185, 232, 264, 296, 543, 837, 885, 1014
DpnII GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
Eco130I CCWWGG 3 cut(s) 483, 633, 927
Eco147I AGGCCT 1 cut(s) 1046
Eco47I GGWCC 2 cut(s) 4, 247
Eco57I CTGAAG 1 cut(s) 531
Eco72I CACGTG 1 cut(s) 889
Eco91I GGTNACC 1 cut(s) 130
EcoO65I GGTNACC 1 cut(s) 130
EcoRII CCWGG 2 cut(s) 446, 493
EcoT14I CCWWGG 3 cut(s) 483, 633, 927
EcoT22I ATGCAT 1 cut(s) 682
ErhI CCWWGG 3 cut(s) 483, 633, 927
FaeI CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 992
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 92
FatI CATG 7 cut(s) 403, 483, 520, 633, 680, 872, 988
FauNDI CATATG 1 cut(s) 676
FbaI TGATCA 3 cut(s) 262, 541, 883
Fnu4HI GCNGC 4 cut(s) 459, 744, 747, 996
FokI GGATG 4 cut(s) 72, 370, 698, 740
Fsp4HI GCNGC 4 cut(s) 459, 744, 747, 996
FspBI CTAG 2 cut(s) 1019, 1050
GluI GCNGC 4 cut(s) 459, 744, 747, 996
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 3 cut(s) 148, 704, 1046
HapII CCGG 2 cut(s) 1079, 1165
Hin1II CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 992
HindIII AAGCTT 3 cut(s) 38, 535, 794
HinfI GANTC 2 cut(s) 1024, 1160
HpaII CCGG 2 cut(s) 1079, 1165
HphI GGTGA 2 cut(s) 425, 1018
Hpy166II GTNNAC 3 cut(s) 573, 661, 862
Hpy188I TCNGA 6 cut(s) 21, 299, 511, 753, 833, 840
Hpy188III TCNNGA 1 cut(s) 1100
Hpy8I GTNNAC 3 cut(s) 573, 661, 862
HpyAV CCTTC 6 cut(s) 152, 431, 506, 1036, 1076, 1097
HpyCH4III ACNGT 1 cut(s) 570
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 8 cut(s) 337, 461, 520, 680, 722, 905, 943, 1095
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 824
HpyF3I CTNAG 3 cut(s) 20, 750, 819
HpySE526I ACGT 1 cut(s) 888
Hsp92II CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 992
Ksp22I TGATCA 3 cut(s) 262, 541, 883
Kzo9I GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
LmnI GCTCC 4 cut(s) 172, 289, 641, 992
Lsp1109I GCAGC 4 cut(s) 445, 733, 755, 1007
LweI GCATC 3 cut(s) 348, 470, 930
MaeI CTAG 2 cut(s) 1019, 1050
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 7 cut(s) 130, 413, 524, 646, 730, 868, 1006
MalI GATC 8 cut(s) 185, 232, 264, 296, 543, 837, 885, 1014
MboI GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
MboII GAAGA 7 cut(s) 74, 421, 600, 766, 910, 964, 977
MflI RGATCY 1 cut(s) 835
MhlI GDGCHC 2 cut(s) 19, 1174
MlyI GAGTC 1 cut(s) 1033
Mph1103I ATGCAT 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 1126
MseI TTAA 7 cut(s) 36, 276, 381, 582, 665, 761, 1068
MslI CAYNNNNRTG 2 cut(s) 408, 679
MspA1I CMGCKG 1 cut(s) 746
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 2 cut(s) 1079, 1165
MspR9I CCNGG 4 cut(s) 448, 495, 1079, 1165
Mva1269I GAATGC 1 cut(s) 1095
MvaI CCWGG 2 cut(s) 448, 495
MwoI GCNNNNNNNGC 2 cut(s) 479, 824
NciI CCSGG 2 cut(s) 1079, 1165
NcoI CCATGG 2 cut(s) 483, 633
NdeI CATATG 1 cut(s) 676
NdeII GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
NlaIII CATG 7 cut(s) 407, 487, 524, 637, 684, 876, 992
NlaIV GGNNCC 4 cut(s) 285, 643, 672, 705
NmuCI GTSAC 3 cut(s) 413, 868, 1006
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 1 cut(s) 876
PceI AGGCCT 1 cut(s) 1046
PctI GAATGC 1 cut(s) 1095
PdmI GAANNNNTTC 1 cut(s) 1126
PfeI GAWTC 1 cut(s) 1160
PflMI CCANNNNNTGG 1 cut(s) 500
PkrI GCNGC 4 cut(s) 460, 745, 748, 997
Ple19I CGATCG 1 cut(s) 186
PleI GAGTC 1 cut(s) 1032
PmaCI CACGTG 1 cut(s) 889
PmlI CACGTG 1 cut(s) 889
PpsI GAGTC 1 cut(s) 1032
Ppu21I YACGTR 1 cut(s) 889
PshBI ATTAAT 1 cut(s) 761
Psp6I CCWGG 2 cut(s) 446, 493
PspCI CACGTG 1 cut(s) 889
PspEI GGTNACC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 446, 493
PspN4I GGNNCC 4 cut(s) 285, 643, 672, 705
PspPI GGNCC 3 cut(s) 4, 247, 703
PstI CTGCAG 1 cut(s) 907
PstNI CAGNNNCTG 1 cut(s) 746
PsuI RGATCY 1 cut(s) 835
PvuI CGATCG 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 746
RsaI GTAC 3 cut(s) 314, 470, 572
RsaNI GTAC 3 cut(s) 313, 469, 571
RseI CAYNNNNRTG 2 cut(s) 408, 679
SaqAI TTAA 7 cut(s) 36, 276, 381, 582, 665, 761, 1068
SatI GCNGC 4 cut(s) 459, 744, 747, 996
Sau3AI GATC 8 cut(s) 183, 230, 262, 294, 541, 835, 883, 1012
Sau96I GGNCC 3 cut(s) 4, 247, 703
SchI GAGTC 1 cut(s) 1033
ScrFI CCNGG 4 cut(s) 448, 495, 1079, 1165
SduI GDGCHC 2 cut(s) 19, 1174
SfaNI GCATC 3 cut(s) 348, 470, 930
SfcI CTRYAG 1 cut(s) 903
SinI GGWCC 2 cut(s) 4, 247
SmiMI CAYNNNNRTG 2 cut(s) 408, 679
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
SseBI AGGCCT 1 cut(s) 1046
SsiI CCGC 3 cut(s) 135, 234, 892
SspMI CTAG 2 cut(s) 1019, 1050
StuI AGGCCT 1 cut(s) 1046
StyD4I CCNGG 4 cut(s) 446, 493, 1077, 1163
StyI CCWWGG 3 cut(s) 483, 633, 927
TaaI ACNGT 1 cut(s) 570
TaiI ACGT 1 cut(s) 891
TaqI TCGA 4 cut(s) 182, 201, 229, 326
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 1 cut(s) 1160
Tru1I TTAA 7 cut(s) 36, 276, 381, 582, 665, 761, 1068
Tru9I TTAA 7 cut(s) 36, 276, 381, 582, 665, 761, 1068
TscAI CASTG 2 cut(s) 912, 981
TseFI GTSAC 3 cut(s) 413, 868, 1006
TseI GCWGC 4 cut(s) 458, 743, 746, 995
Tsp45I GTSAC 3 cut(s) 413, 868, 1006
TspDTI ATGAA 3 cut(s) 74, 957, 977
TspRI CASTG 2 cut(s) 912, 981
Van91I CCANNNNNTGG 1 cut(s) 500
Vha464I CTTAAG 1 cut(s) 35
VpaK11BI GGWCC 2 cut(s) 4, 247
VspI ATTAAT 1 cut(s) 761
XapI RAATTY 3 cut(s) 322, 339, 1122
XceI RCATGY 1 cut(s) 876
XmnI GAANNNNTTC 1 cut(s) 1126
XspI CTAG 2 cut(s) 1019, 1050
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.