Rh4AG337500

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001162
Physical Location & Seq
Forward (+)
64655741 .. 64658260
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG337500.1

Sequence Viewer

Length: 1311 bp
ATGCTCCATGTGCATCTTCCAGAGACAGCGATGTCCACATTGGACGGTCAAAACTCTAGCATCAACCTTAGTCATCTGACTCAGTTTCATTCGAACTCTGAGAGCTCCGTGCTTAACGTTTTCCTCACCAAACTCACCATGGAACTTTCTCGAGCTCTGAGATTCCAGCAACTGGAGCTTCCTTTCTGCCCCCAACAACTCCAAACCCAGAGGCCTACATTTTCAGTCGCCGCCAGAAGAACAAGTCAGATAATAGTTGCAGCGGTCAAGACCAAGAGAAGAAGGCCACCCATTGAAGGCGTTAGCGAGGAGCTCAATTTGATCGCCGATCAGAGCCTTGACTGTGCTCCGGCTCGGAGAAGAGTTCGATCCGCATTCGTTGAGCTGCAGCAGCAACTTGATCACTGCTTGTTTAAGTTGGCTCCAACTGGGATCAGAACAGAAGAGTGGTACGAAATAAATTCAAGGGGGCTAGGAATTTTCTGTAAAAGCTGGAAGCCCAGAGAAGGGGTTCCGATTAAAGGCGTATTGTGTTTCTGCCATGGTTATGGTGATACTTGCGTTTTCTTCTTTGAAGGTATTGCCAAGCAGATTGCGGCAGCTGGGTACGCTGTGTACGCCATGGACTACCCAGGCTTTGGCCTTTCAGAAGGATTGCATGGTTATATCCCGAGCTTTGATCAGCTAGCTGACGATGTTATTGAACAGTATACAAAGATTAAAGCAAAACCTGAGTTGAAGGGGTTGCCTCACTTCATATTGGGCCAGTCCATGGGTGGAGCTGTTACTCTCAAAGTTCACTTTAAGCAACCATATGCATGGGATGGCATAGTTCTTGTGGCTCCAATGTGTAAAATTGCAGAGGATGTAACACCTCCACCAGCGGCAGCGAAGCTATTAACTCTTATGTCCAATGTTGTCCCCAAAGCAAAGCTTGTCCCGCAGAAAGACTTGGCCGAGCTGGCCTTCAGAGATGAAAGGAAAAGGAAACTGGCTGTCTACAATGTGACATGCTACAATGGCCCCGCGCGATTGAAGACTGCTGTGGAACTACTAAACGCTACTAAGGAGATTGAGATGCAAGTGCATAAGGTTTCATCTCCACTGCTAGTTCTTCATGGAGCTGCAGATAAGGTGACTGATCCCTTAGTGAGCCAGTTTCTTTATGAGAAGGCCTCAAGCAAAGACAAAACTCTAAAACTCTACCCAGATGGCTTTCATTGCATTCTTGAAGGAGAACCTGATGATAGAATCTTTTCAGTCCTTGAAGATATTATCACTTGGCTTGATCACCGGTGTTTGCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

436

Amino Acids

48.75

Weight (kDa)

8.38

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 173 - 414 4.7e-66 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 176 - 413 2.1e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 179 - 417 7.8e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 31
AccB7I CCANNNNNTGG 3 cut(s) 172, 638, 772
AccI GTMKAC 2 cut(s) 710, 999
AccII CGCG 2 cut(s) 1028, 1030
AciI CCGC 7 cut(s) 231, 263, 372, 596, 884, 941, 1026
AclI AACGTT 1 cut(s) 117
AclWI GGATC 3 cut(s) 363, 440, 1136
AcoI YGGCCR 1 cut(s) 954
AcsI RAATTY 2 cut(s) 460, 477
AcuI CTGAAG 1 cut(s) 952
AfaI GTAC 3 cut(s) 452, 608, 617
AfiI CCNNNNNNNGG 7 cut(s) 172, 296, 506, 507, 521, 638, 772
AgeI ACCGGT 1 cut(s) 1293
AgsI TTSAA 8 cut(s) 296, 465, 575, 704, 739, 1036, 1232, 1268
AjnI CCWGG 1 cut(s) 631
Alw21I GWGCWC 4 cut(s) 107, 157, 315, 349
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 3 cut(s) 363, 440, 1136
AlwNI CAGNNNCTG 1 cut(s) 172
Ama87I CYCGRG 2 cut(s) 150, 670
AoxI GGCC 8 cut(s) 212, 284, 640, 763, 954, 963, 1021, 1173
ApeKI GCWGC 7 cut(s) 260, 385, 388, 391, 599, 887, 1124
ApoI RAATTY 2 cut(s) 460, 477
AsiGI ACCGGT 1 cut(s) 1293
Asp700I GAANNNNTTC 2 cut(s) 510, 1255
AspLEI GCGC 1 cut(s) 1030
AspS9I GGNCC 2 cut(s) 763, 1022
AsuHPI GGTGA 5 cut(s) 118, 127, 563, 1147, 1283
AsuII TTCGAA 1 cut(s) 92
AsuNHI GCTAGC 1 cut(s) 685
AvaI CYCGRG 2 cut(s) 150, 670
BaeI ACNNNNGTAYC 2 cut(s) 546, 579
BanII GRGCYC 3 cut(s) 107, 157, 315
BbsI GAAGAC 1 cut(s) 1043
Bbv12I GWGCWC 4 cut(s) 107, 157, 315, 349
BbvI GCAGC 7 cut(s) 272, 372, 400, 403, 611, 899, 1111
BccI CCATC 2 cut(s) 818, 1205
BciT130I CCWGG 1 cut(s) 633
BclI TGATCA 3 cut(s) 400, 679, 1288
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 4 cut(s) 57, 473, 686, 1109
BfmI CTRYAG 2 cut(s) 386, 1125
BglI GCCNNNNNGGC 1 cut(s) 962
Bme1390I CCNGG 1 cut(s) 633
BmeT110I CYCGRG 2 cut(s) 150, 670
BmgT120I GGNCC 2 cut(s) 763, 1022
BmiI GGNNCC 4 cut(s) 423, 513, 843, 1024
BmrFI CCNGG 1 cut(s) 633
BmrI ACTGGG 1 cut(s) 438
BmsI GCATC 3 cut(s) 22, 69, 1068
BmtI GCTAGC 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 1043
BplI GAGNNNNNCTC 2 cut(s) 1160, 1192
BpmI CTGGAG 1 cut(s) 194
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 2 cut(s) 1162, 1289
BsaJI CCNNGG 5 cut(s) 138, 541, 621, 631, 771
BsaWI WCCGGW 1 cut(s) 1293
Bsc4I CCNNNNNNNGG 7 cut(s) 172, 296, 506, 507, 521, 638, 772
Bse118I RCCGGY 1 cut(s) 1293
Bse1I ACTGG 5 cut(s) 177, 433, 766, 996, 1156
Bse3DI GCAATG 1 cut(s) 1219
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 5 cut(s) 138, 541, 621, 631, 771
BseGI GGATG 2 cut(s) 829, 871
BseLI CCNNNNNNNGG 7 cut(s) 172, 296, 506, 507, 521, 638, 772
BseMI GCAATG 1 cut(s) 1219
BseMII CTCAG 4 cut(s) 90, 95, 149, 723
BseNI ACTGG 5 cut(s) 177, 433, 766, 996, 1156
BseRI GAGGAG 1 cut(s) 323
BseXI GCAGC 7 cut(s) 272, 372, 400, 403, 611, 899, 1111
BseYI CCCAGC 1 cut(s) 602
Bsh1236I CGCG 2 cut(s) 1028, 1030
BshFI GGCC 8 cut(s) 214, 286, 642, 765, 956, 965, 1023, 1175
BshTI ACCGGT 1 cut(s) 1293
BsiHKAI GWGCWC 4 cut(s) 107, 157, 315, 349
BsiHKCI CYCGRG 2 cut(s) 150, 670
BsiSI CCGG 2 cut(s) 350, 1294
BslFI GGGAC 2 cut(s) 905, 923
BslI CCNNNNNNNGG 7 cut(s) 172, 296, 506, 507, 521, 638, 772
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 2 cut(s) 905, 923
BsmI GAATGC 2 cut(s) 374, 1224
BsnI GGCC 8 cut(s) 214, 286, 642, 765, 956, 965, 1023, 1175
BsoBI CYCGRG 2 cut(s) 150, 670
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 4 cut(s) 107, 157, 315, 349
Bsp143I GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
Bsp19I CCATGG 4 cut(s) 138, 541, 621, 771
BspACI CCGC 7 cut(s) 231, 263, 372, 596, 884, 941, 1026
BspANI GGCC 8 cut(s) 214, 286, 642, 765, 956, 965, 1023, 1175
BspCNI CTCAG 4 cut(s) 91, 94, 150, 724
BspFNI CGCG 2 cut(s) 1028, 1030
BspLI GGNNCC 4 cut(s) 423, 513, 843, 1024
BspMAI CTGCAG 2 cut(s) 390, 1129
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 3 cut(s) 363, 440, 1136
BspT104I TTCGAA 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 1219
BsrFI RCCGGY 1 cut(s) 1293
BsrI ACTGG 5 cut(s) 177, 433, 766, 996, 1156
BssAI RCCGGY 1 cut(s) 1293
BssECI CCNNGG 5 cut(s) 138, 541, 621, 631, 771
BssMI GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
BssNAI GTATAC 1 cut(s) 711
BssT1I CCWWGG 4 cut(s) 138, 541, 621, 771
Bst1107I GTATAC 1 cut(s) 711
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 3 cut(s) 47, 344, 708
Bst6I CTCTTC 2 cut(s) 355, 438
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 2 cut(s) 687, 963
BstDEI CTNAG 7 cut(s) 68, 81, 99, 158, 732, 1065, 1147
BstDSI CCRYGG 4 cut(s) 138, 541, 621, 771
BstF5I GGATG 2 cut(s) 829, 871
BstFNI CGCG 2 cut(s) 1028, 1030
BstHHI GCGC 1 cut(s) 1030
BstKTI GATC 8 cut(s) 324, 331, 371, 403, 435, 682, 1144, 1291
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
BstMWI GCNNNNNNNGC 9 cut(s) 10, 175, 391, 608, 617, 940, 962, 1020, 1221
BstNI CCWGG 1 cut(s) 633
BstNSI RCATGY 1 cut(s) 1014
BstSCI CCNGG 1 cut(s) 631
BstSFI CTRYAG 2 cut(s) 386, 1125
BstUI CGCG 2 cut(s) 1028, 1030
BstV1I GCAGC 7 cut(s) 272, 372, 400, 403, 611, 899, 1111
BstV2I GAAGAC 1 cut(s) 1043
BstXI CCANNNNNNTGG 2 cut(s) 548, 819
BstZ17I GTATAC 1 cut(s) 711
BsuRI GGCC 8 cut(s) 214, 286, 642, 765, 956, 965, 1023, 1175
BtgI CCRYGG 4 cut(s) 138, 541, 621, 771
BtgZI GCGATG 1 cut(s) 44
BtsCI GGATG 2 cut(s) 829, 871
BtsI GCAGTG 2 cut(s) 403, 1103
BtsIMutI CAGTG 2 cut(s) 403, 1103
Cac8I GCNNGC 2 cut(s) 687, 963
CaiI CAGNNNCTG 1 cut(s) 172
CfoI GCGC 1 cut(s) 1030
Cfr10I RCCGGY 1 cut(s) 1293
Cfr13I GGNCC 2 cut(s) 763, 1022
Csp6I GTAC 3 cut(s) 451, 607, 616
CspAI ACCGGT 1 cut(s) 1293
CviAII CATG 9 cut(s) 8, 139, 542, 622, 659, 772, 819, 1011, 1118
CviQI GTAC 3 cut(s) 451, 607, 616
DdeI CTNAG 7 cut(s) 68, 81, 99, 158, 732, 1065, 1147
DpnI GATC 8 cut(s) 323, 330, 370, 402, 434, 681, 1143, 1290
DpnII GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 954
Eam1104I CTCTTC 2 cut(s) 355, 438
EarI CTCTTC 2 cut(s) 355, 438
Ecl136II GAGCTC 3 cut(s) 105, 155, 313
Eco130I CCWWGG 4 cut(s) 138, 541, 621, 771
Eco147I AGGCCT 2 cut(s) 214, 1175
Eco24I GRGCYC 3 cut(s) 107, 157, 315
Eco53kI GAGCTC 3 cut(s) 105, 155, 313
Eco57I CTGAAG 1 cut(s) 952
Eco88I CYCGRG 2 cut(s) 150, 670
EcoICRI GAGCTC 3 cut(s) 105, 155, 313
EcoRII CCWGG 1 cut(s) 631
EcoT14I CCWWGG 4 cut(s) 138, 541, 621, 771
EcoT22I ATGCAT 1 cut(s) 820
EcoT38I GRGCYC 3 cut(s) 107, 157, 315
ErhI CCWWGG 4 cut(s) 138, 541, 621, 771
FaeI CATG 9 cut(s) 11, 142, 545, 625, 662, 775, 822, 1014, 1121
FalI AAGNNNNNCTT 2 cut(s) 918, 950
FaqI GGGAC 2 cut(s) 905, 923
FatI CATG 9 cut(s) 7, 138, 541, 621, 658, 771, 818, 1010, 1117
FauI CCCGC 2 cut(s) 948, 1033
FauNDI CATATG 1 cut(s) 814
FbaI TGATCA 3 cut(s) 400, 679, 1288
FblI GTMKAC 2 cut(s) 710, 999
FokI GGATG 2 cut(s) 836, 878
FriOI GRGCYC 3 cut(s) 107, 157, 315
FspBI CTAG 4 cut(s) 57, 473, 686, 1109
GlaI GCGC 1 cut(s) 1029
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 1 cut(s) 194
HaeIII GGCC 8 cut(s) 214, 286, 642, 765, 956, 965, 1023, 1175
HapII CCGG 2 cut(s) 350, 1294
HhaI GCGC 1 cut(s) 1030
Hin1II CATG 9 cut(s) 11, 142, 545, 625, 662, 775, 822, 1014, 1121
Hin6I GCGC 1 cut(s) 1028
HinP1I GCGC 1 cut(s) 1028
HindIII AAGCTT 1 cut(s) 932
HinfI GANTC 3 cut(s) 79, 162, 1251
HpaII CCGG 2 cut(s) 350, 1294
HphI GGTGA 5 cut(s) 118, 127, 563, 1147, 1283
Hpy166II GTNNAC 5 cut(s) 36, 616, 711, 799, 1000
Hpy188III TCNNGA 5 cut(s) 20, 150, 268, 670, 1229
Hpy8I GTNNAC 5 cut(s) 36, 616, 711, 799, 1000
HpyAV CCTTC 9 cut(s) 276, 290, 500, 569, 644, 733, 976, 1165, 1226
HpyCH4III ACNGT 3 cut(s) 47, 344, 708
HpyCH4IV ACGT 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 175, 391, 608, 617, 940, 962, 1020, 1221
HpyF3I CTNAG 7 cut(s) 68, 81, 99, 158, 732, 1065, 1147
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 9 cut(s) 11, 142, 545, 625, 662, 775, 822, 1014, 1121
HspAI GCGC 1 cut(s) 1028
Ksp22I TGATCA 3 cut(s) 400, 679, 1288
Kzo9I GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
LmnI GCTCC 9 cut(s) 9, 110, 175, 310, 352, 427, 779, 847, 1121
Lsp1109I GCAGC 7 cut(s) 272, 372, 400, 403, 611, 899, 1111
LweI GCATC 3 cut(s) 22, 69, 1068
MaeI CTAG 4 cut(s) 57, 473, 686, 1109
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 4 cut(s) 784, 868, 1006, 1135
MalI GATC 8 cut(s) 323, 330, 370, 402, 434, 681, 1143, 1290
MboI GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
MboII GAAGA 9 cut(s) 8, 249, 291, 372, 455, 559, 1048, 1106, 1280
MhlI GDGCHC 4 cut(s) 107, 157, 315, 349
MluCI AATT 4 cut(s) 316, 460, 477, 855
MlyI GAGTC 1 cut(s) 73
MmeI TCCRAC 1 cut(s) 449
MnlI CCTC 7 cut(s) 134, 204, 301, 759, 856, 885, 1186
Mph1103I ATGCAT 1 cut(s) 820
MroXI GAANNNNTTC 2 cut(s) 510, 1255
MseI TTAA 6 cut(s) 114, 414, 519, 720, 804, 899
MslI CAYNNNNRTG 2 cut(s) 546, 817
MspA1I CMGCKG 3 cut(s) 263, 602, 884
MspI CCGG 2 cut(s) 350, 1294
MspR9I CCNGG 1 cut(s) 633
Mva1269I GAATGC 2 cut(s) 374, 1224
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 2 cut(s) 1028, 1030
MwoI GCNNNNNNNGC 9 cut(s) 10, 175, 391, 608, 617, 940, 962, 1020, 1221
NcoI CCATGG 4 cut(s) 138, 541, 621, 771
NdeI CATATG 1 cut(s) 814
NdeII GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
NheI GCTAGC 1 cut(s) 685
NlaIII CATG 9 cut(s) 11, 142, 545, 625, 662, 775, 822, 1014, 1121
NlaIV GGNNCC 4 cut(s) 423, 513, 843, 1024
NmeAIII GCCGAG 1 cut(s) 982
NmuCI GTSAC 2 cut(s) 1006, 1135
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 1 cut(s) 1014
NspV TTCGAA 1 cut(s) 92
PaeR7I CTCGAG 1 cut(s) 150
PceI AGGCCT 2 cut(s) 214, 1175
PctI GAATGC 2 cut(s) 374, 1224
PdmI GAANNNNTTC 2 cut(s) 510, 1255
PfeI GAWTC 2 cut(s) 162, 1251
PflMI CCANNNNNTGG 3 cut(s) 172, 638, 772
PinAI ACCGGT 1 cut(s) 1293
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
Psp124BI GAGCTC 3 cut(s) 107, 157, 315
Psp1406I AACGTT 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 4 cut(s) 423, 513, 843, 1024
PspPI GGNCC 2 cut(s) 763, 1022
PstI CTGCAG 2 cut(s) 390, 1129
PstNI CAGNNNCTG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 3 cut(s) 452, 608, 617
RsaNI GTAC 3 cut(s) 451, 607, 616
RseI CAYNNNNRTG 2 cut(s) 546, 817
SacI GAGCTC 3 cut(s) 107, 157, 315
SaqAI TTAA 6 cut(s) 114, 414, 519, 720, 804, 899
Sau3AI GATC 8 cut(s) 321, 328, 368, 400, 432, 679, 1141, 1288
Sau96I GGNCC 2 cut(s) 763, 1022
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 633
SduI GDGCHC 4 cut(s) 107, 157, 315, 349
SfaNI GCATC 3 cut(s) 22, 69, 1068
SfcI CTRYAG 2 cut(s) 386, 1125
SfiI GGCCNNNNNGGCC 1 cut(s) 962
Sfr274I CTCGAG 1 cut(s) 150
SfuI TTCGAA 1 cut(s) 92
SgrAI CRCCGGYG 1 cut(s) 1293
SlaI CTCGAG 1 cut(s) 150
SmiMI CAYNNNNRTG 2 cut(s) 546, 817
SmlI CTYRAG 3 cut(s) 150, 1177, 1304
SmoI CTYRAG 3 cut(s) 150, 1177, 1304
Sse9I AATT 4 cut(s) 316, 460, 477, 855
SseBI AGGCCT 2 cut(s) 214, 1175
SsiI CCGC 7 cut(s) 231, 263, 372, 596, 884, 941, 1026
SspMI CTAG 4 cut(s) 57, 473, 686, 1109
SstI GAGCTC 3 cut(s) 107, 157, 315
StuI AGGCCT 2 cut(s) 214, 1175
StyD4I CCNGG 1 cut(s) 631
StyI CCWWGG 4 cut(s) 138, 541, 621, 771
TaaI ACNGT 3 cut(s) 47, 344, 708
TaiI ACGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 92, 151, 367
TasI AATT 4 cut(s) 316, 460, 477, 855
TauI GCSGC 3 cut(s) 233, 599, 887
TfiI GAWTC 2 cut(s) 162, 1251
Tru1I TTAA 6 cut(s) 114, 414, 519, 720, 804, 899
Tru9I TTAA 6 cut(s) 114, 414, 519, 720, 804, 899
TscAI CASTG 2 cut(s) 410, 1110
TseFI GTSAC 2 cut(s) 1006, 1135
TseI GCWGC 7 cut(s) 260, 385, 388, 391, 599, 887, 1124
Tsp45I GTSAC 2 cut(s) 1006, 1135
TspDTI ATGAA 6 cut(s) 77, 745, 990, 1086, 1106, 1208
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 2 cut(s) 410, 1110
Van91I CCANNNNNTGG 3 cut(s) 172, 638, 772
XapI RAATTY 2 cut(s) 460, 477
XceI RCATGY 1 cut(s) 1014
XcmI CCANNNNNNNNNTGG 2 cut(s) 136, 773
XhoI CTCGAG 1 cut(s) 150
XmiI GTMKAC 2 cut(s) 710, 999
XmnI GAANNNNTTC 2 cut(s) 510, 1255
XspI CTAG 4 cut(s) 57, 473, 686, 1109
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.