MD16G1041900.v1.1

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001162
Physical Location & Seq
Forward (+)
2949505 .. 2957478
7974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1041900.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGATCCTGTCACCTACTCCGACTTTCCGACCACCAACCCCCCCTCCGTTTCACCGGAAATCAAGCTCTCCGAGCAAACAACCACAAAGGAAGCACCTACCCTCTTTCCTCCCATCAAAATCGTATCCGAAAACCCCAAAAACTCACTTCAGAGTTACCGCCTGGAAACGGTTGCCAACGGAAGGGTTAAGTCAAGAGATGTGCGCCATAGCTTCGCAGAATCTGGACCATGCGCCGGCTCGAAGACGAGTCCGGTCCGCATTCATCGACGTGCATCAGCAACTTGATCATTGCTTGTTCAAGATGGATCATGGTGGCATCAGAACAGAGGAGTGGTATGGAAGGAATTCAAGGGGTATGGAGATTTTCTGCAAAAGCTGGTTGCCAAAAGTTGGTGATCGAATCAAAGGTGCTTTATGCTTCTGCCATGGCTATGGTAGTACTTGCACATTTTTCTTTGGAGGTATCGCCAAGCGAATTGCTGCATCCGGATACGCTATATACGCCGTGGACTATCCGGGTTTCGGTCTTTCTGAAGGATTGCATGGCTACATCCCAAACTTTGATGAATTAGTCGGCGATGTAATCGAACAGTTTACTGATGTCAAAGGAAGATCTGAAGTGAAAGGCCTGCCATTCTTTGTAATGGGAGAATCCATGGGAGGAGCCGTGAGTCTCAAGATAAATCTTAAGGAGCCGGACAAATGGGATGGAGTAATCCTTGTAGCTCCAATGTGCAAAATTGCGGAAGACGTGATGCCTCCGGCAATAGTACTGAAGCTATTAGCCCTGATGTCTGAAGTTTTGCCTGAGGCGAAACTCTTCCCGCAGAAAGATACATCCGAGCTAAGCTACAGAGATCCGAAGAACAGAAAAACGGCCGGTTACAATGTGATTTCTTACAAGGACAACATGCGATCAAGAACAGCTGTGGAACTTCTGAAGGCCACAAGTGATATCGAAACGCAGTTGGATAAGGTTTCGGGCCCGGTTCTAATTCTTCATGGAGCAGAAGATAAGGTGACGGATCCTCTGGTGAGCCGGTTGCTTTACGAGAAGGCATCTAGCAAGGATAAAACTTTGAAGCTCTATCCGGATGGTTTTCACTGCATTCTTCAAGGGGAGCCCGATGACAGAATTTTCACTGTTCTTGATGACATTGTAACATGGCTTGATTCCCGTTGCTCCCCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

399

Amino Acids

44.54

Weight (kDa)

8.8

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 135 - 376 1.4e-62 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 138 - 372 6.2e-16 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 141 - 378 4.1e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 392
AccIII TCCGGA 2 cut(s) 488, 1093
AciI CCGC 4 cut(s) 159, 258, 746, 827
AclWI GGATC 4 cut(s) 315, 854, 1022, 1035
AcoI YGGCCR 1 cut(s) 879
AcsI RAATTY 2 cut(s) 346, 1137
AcuI CTGAAG 6 cut(s) 133, 555, 639, 797, 819, 962
AfaI GTAC 2 cut(s) 442, 774
AfiI CCNNNNNNNGG 5 cut(s) 168, 182, 235, 392, 524
AflII CTTAAG 1 cut(s) 689
AgsI TTSAA 4 cut(s) 301, 351, 1084, 1118
AjiI CACGTC 2 cut(s) 271, 754
AjnI CCWGG 1 cut(s) 161
AluBI AGCT 9 cut(s) 66, 212, 378, 728, 781, 847, 852, 929, 1087
AluI AGCT 9 cut(s) 66, 212, 378, 728, 781, 847, 852, 929, 1087
Alw26I GTCTC 1 cut(s) 680
AlwI GGATC 4 cut(s) 315, 854, 1022, 1035
AlwNI CAGNNNCTG 1 cut(s) 223
Aor13HI TCCGGA 2 cut(s) 488, 1093
AoxI GGCC 4 cut(s) 628, 879, 945, 985
ApaI GGGCCC 1 cut(s) 989
ApeKI GCWGC 1 cut(s) 482
ApoI RAATTY 2 cut(s) 346, 1137
Asp700I GAANNNNTTC 2 cut(s) 346, 821
AspLEI GCGC 2 cut(s) 206, 235
AspS9I GGNCC 4 cut(s) 226, 255, 985, 986
AsuC2I CCSGG 2 cut(s) 519, 989
AsuHPI GGTGA 5 cut(s) 3, 44, 407, 1033, 1048
AvaII GGWCC 2 cut(s) 226, 255
AxyI CCTNAGG 1 cut(s) 810
BaeGI GKGCMC 1 cut(s) 989
BamHI GGATCC 1 cut(s) 1027
BanII GRGCYC 2 cut(s) 989, 1128
BbsI GAAGAC 2 cut(s) 250, 756
BbvI GCAGC 1 cut(s) 469
BccI CCATC 4 cut(s) 121, 298, 704, 1091
BceAI ACGGC 3 cut(s) 491, 653, 894
BciT130I CCWGG 1 cut(s) 163
BciVI GTATCC 2 cut(s) 135, 485
BclI TGATCA 1 cut(s) 286
BcnI CCSGG 2 cut(s) 519, 989
BcoDI GTCTC 1 cut(s) 680
BfaI CTAG 1 cut(s) 1065
BfmI CTRYAG 1 cut(s) 853
BfrI CTTAAG 1 cut(s) 689
BfuI GTATCC 2 cut(s) 135, 485
BglII AGATCT 1 cut(s) 614
BisI GCNGC 1 cut(s) 483
BlpI GCTNAGC 1 cut(s) 848
BlsI GCNGC 1 cut(s) 484
BmcAI AGTACT 2 cut(s) 442, 774
Bme1390I CCNGG 3 cut(s) 163, 519, 989
Bme18I GGWCC 2 cut(s) 226, 255
BmgBI CACGTC 2 cut(s) 271, 754
BmgT120I GGNCC 4 cut(s) 226, 255, 985, 986
BmiI GGNNCC 5 cut(s) 667, 696, 987, 1029, 1125
BmrFI CCNGG 3 cut(s) 163, 519, 989
BmsI GCATC 5 cut(s) 283, 327, 494, 747, 1070
BpiI GAAGAC 2 cut(s) 250, 756
Bpu1102I GCTNAGC 1 cut(s) 848
BpuEI CTTGAG 1 cut(s) 662
BpuMI CCSGG 2 cut(s) 519, 989
BsaJI CCNNGG 3 cut(s) 427, 507, 657
BsaWI WCCGGW 4 cut(s) 54, 252, 488, 1093
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 654, 684
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 182, 235, 392, 524
Bse118I RCCGGY 3 cut(s) 235, 881, 1041
Bse21I CCTNAGG 1 cut(s) 810
Bse3DI GCAATG 1 cut(s) 289
BseAI TCCGGA 2 cut(s) 488, 1093
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 3 cut(s) 427, 507, 657
BseGI GGATG 5 cut(s) 485, 552, 715, 839, 1102
BseLI CCNNNNNNNGG 5 cut(s) 168, 182, 235, 392, 524
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 801
BseRI GAGGAG 2 cut(s) 344, 678
BseSI GKGCMC 1 cut(s) 989
BseX3I CGGCCG 1 cut(s) 879
BseXI GCAGC 1 cut(s) 469
Bsh1285I CGRYCG 1 cut(s) 882
BshFI GGCC 4 cut(s) 630, 881, 947, 987
BsiEI CGRYCG 1 cut(s) 882
BslI CCNNNNNNNGG 5 cut(s) 168, 182, 235, 392, 524
BsmAI GTCTC 1 cut(s) 680
BsmI GAATGC 2 cut(s) 260, 1110
BsnI GGCC 4 cut(s) 630, 881, 947, 987
Bsp120I GGGCCC 1 cut(s) 985
Bsp1286I GDGCHC 2 cut(s) 989, 1128
Bsp13I TCCGGA 2 cut(s) 488, 1093
Bsp143I GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
Bsp1720I GCTNAGC 1 cut(s) 848
Bsp19I CCATGG 2 cut(s) 427, 657
BspACI CCGC 4 cut(s) 159, 258, 746, 827
BspANI GGCC 4 cut(s) 630, 881, 947, 987
BspCNI CTCAG 1 cut(s) 802
BspEI TCCGGA 2 cut(s) 488, 1093
BspLI GGNNCC 5 cut(s) 667, 696, 987, 1029, 1125
BspPI GGATC 4 cut(s) 315, 854, 1022, 1035
BspTI CTTAAG 1 cut(s) 689
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 3 cut(s) 235, 881, 1041
BssAI RCCGGY 3 cut(s) 235, 881, 1041
BssECI CCNNGG 3 cut(s) 427, 507, 657
BssMI GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
BssT1I CCWWGG 2 cut(s) 427, 657
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 3 cut(s) 171, 594, 1147
Bst6I CTCTTC 1 cut(s) 827
BstAFI CTTAAG 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 237, 632
BstDEI CTNAG 2 cut(s) 810, 848
BstDSI CCRYGG 3 cut(s) 427, 507, 657
BstF5I GGATG 5 cut(s) 485, 552, 715, 839, 1102
BstHHI GCGC 2 cut(s) 206, 235
BstKTI GATC 8 cut(s) 6, 289, 310, 400, 617, 862, 920, 1030
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
BstMCI CGRYCG 1 cut(s) 882
BstMWI GCNNNNNNNGC 2 cut(s) 72, 503
BstNI CCWGG 1 cut(s) 163
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 3 cut(s) 161, 517, 987
BstSFI CTRYAG 1 cut(s) 853
BstSLI GKGCMC 1 cut(s) 989
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 2 cut(s) 250, 756
BstX2I RGATCY 3 cut(s) 614, 859, 1027
BstXI CCANNNNNNTGG 1 cut(s) 434
BstYI RGATCY 3 cut(s) 614, 859, 1027
BstZI CGGCCG 1 cut(s) 879
Bsu36I CCTNAGG 1 cut(s) 810
BsuI GTATCC 2 cut(s) 135, 485
BsuRI GGCC 4 cut(s) 630, 881, 947, 987
BtgI CCRYGG 3 cut(s) 427, 507, 657
BtgZI GCGATG 1 cut(s) 594
BtrI CACGTC 2 cut(s) 271, 754
BtsCI GGATG 5 cut(s) 485, 552, 715, 839, 1102
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 2 cut(s) 1105, 1143
Cac8I GCNNGC 2 cut(s) 237, 632
CaiI CAGNNNCTG 1 cut(s) 223
CfoI GCGC 2 cut(s) 206, 235
Cfr10I RCCGGY 3 cut(s) 235, 881, 1041
Cfr13I GGNCC 4 cut(s) 226, 255, 985, 986
CpoI CGGWCCG 1 cut(s) 255
Csp6I GTAC 2 cut(s) 441, 773
CspI CGGWCCG 1 cut(s) 255
CviAII CATG 8 cut(s) 230, 311, 428, 545, 658, 913, 1004, 1167
CviQI GTAC 2 cut(s) 441, 773
DdeI CTNAG 2 cut(s) 810, 848
DpnI GATC 8 cut(s) 5, 288, 309, 399, 616, 861, 919, 1029
DpnII GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
EaeI YGGCCR 1 cut(s) 879
EagI CGGCCG 1 cut(s) 879
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
EclXI CGGCCG 1 cut(s) 879
Eco130I CCWWGG 2 cut(s) 427, 657
Eco147I AGGCCT 1 cut(s) 630
Eco24I GRGCYC 2 cut(s) 989, 1128
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 226, 255
Eco52I CGGCCG 1 cut(s) 879
Eco57I CTGAAG 6 cut(s) 133, 555, 639, 797, 819, 962
Eco81I CCTNAGG 1 cut(s) 810
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 161
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 2 cut(s) 427, 657
EcoT38I GRGCYC 2 cut(s) 989, 1128
ErhI CCWWGG 2 cut(s) 427, 657
FaeI CATG 8 cut(s) 233, 314, 431, 548, 661, 916, 1007, 1170
FatI CATG 8 cut(s) 229, 310, 427, 544, 657, 912, 1003, 1166
FauI CCCGC 1 cut(s) 834
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 483
FokI GGATG 5 cut(s) 472, 539, 722, 826, 1109
FriOI GRGCYC 2 cut(s) 989, 1128
Fsp4HI GCNGC 1 cut(s) 483
FspBI CTAG 1 cut(s) 1065
GlaI GCGC 2 cut(s) 205, 234
GluI GCNGC 1 cut(s) 483
HaeIII GGCC 4 cut(s) 630, 881, 947, 987
HhaI GCGC 2 cut(s) 206, 235
Hin1II CATG 8 cut(s) 233, 314, 431, 548, 661, 916, 1007, 1170
Hin6I GCGC 2 cut(s) 204, 233
HinP1I GCGC 2 cut(s) 204, 233
HinfI GANTC 6 cut(s) 220, 249, 402, 653, 673, 1175
HphI GGTGA 5 cut(s) 3, 44, 407, 1033, 1048
Hpy166II GTNNAC 2 cut(s) 511, 597
Hpy188III TCNNGA 8 cut(s) 194, 224, 301, 489, 679, 921, 1094, 1151
Hpy8I GTNNAC 2 cut(s) 511, 597
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 5 cut(s) 176, 336, 530, 937, 1051
HpyCH4III ACNGT 3 cut(s) 171, 594, 1147
HpyCH4IV ACGT 2 cut(s) 270, 753
HpyCH4V TGCA 7 cut(s) 274, 372, 447, 485, 544, 738, 1110
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 503
HpyF3I CTNAG 2 cut(s) 810, 848
HpySE526I ACGT 2 cut(s) 270, 753
Hsp92II CATG 8 cut(s) 233, 314, 431, 548, 661, 916, 1007, 1170
HspAI GCGC 2 cut(s) 204, 233
Kpn2I TCCGGA 2 cut(s) 488, 1093
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
LmnI GCTCC 6 cut(s) 665, 694, 733, 1007, 1123, 1190
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 5 cut(s) 283, 327, 494, 747, 1070
MaeI CTAG 1 cut(s) 1065
MaeII ACGT 2 cut(s) 270, 753
MaeIII GTNAC 5 cut(s) 9, 154, 884, 1021, 1162
MalI GATC 8 cut(s) 5, 288, 309, 399, 616, 861, 919, 1029
MboI GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
MboII GAAGA 8 cut(s) 255, 624, 761, 814, 877, 992, 1025, 1106
MflI RGATCY 3 cut(s) 614, 859, 1027
MhlI GDGCHC 2 cut(s) 989, 1128
MluCI AATT 7 cut(s) 346, 477, 569, 741, 996, 1137, 1192
MlyI GAGTC 2 cut(s) 258, 682
MmeI TCCRAC 3 cut(s) 44, 52, 951
MnlI CCTC 9 cut(s) 54, 112, 119, 322, 455, 656, 771, 805, 1041
MroI TCCGGA 2 cut(s) 488, 1093
MroNI GCCGGC 1 cut(s) 235
MroXI GAANNNNTTC 2 cut(s) 346, 821
MseI TTAA 3 cut(s) 188, 690, 1195
MslI CAYNNNNRTG 2 cut(s) 269, 432
MspA1I CMGCKG 1 cut(s) 929
MspCI CTTAAG 1 cut(s) 689
MspR9I CCNGG 3 cut(s) 163, 519, 989
Mva1269I GAATGC 2 cut(s) 260, 1110
MvaI CCWGG 1 cut(s) 163
MwoI GCNNNNNNNGC 2 cut(s) 72, 503
NaeI GCCGGC 1 cut(s) 237
NciI CCSGG 2 cut(s) 519, 989
NcoI CCATGG 2 cut(s) 427, 657
NdeII GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
NgoMIV GCCGGC 1 cut(s) 235
NlaIII CATG 8 cut(s) 233, 314, 431, 548, 661, 916, 1007, 1170
NlaIV GGNNCC 5 cut(s) 667, 696, 987, 1029, 1125
NmuCI GTSAC 2 cut(s) 9, 1021
NspI RCATGY 1 cut(s) 916
PceI AGGCCT 1 cut(s) 630
PctI GAATGC 2 cut(s) 260, 1110
PdiI GCCGGC 1 cut(s) 237
PdmI GAANNNNTTC 2 cut(s) 346, 821
PfeI GAWTC 4 cut(s) 220, 402, 653, 1175
PflMI CCANNNNNTGG 1 cut(s) 392
PkrI GCNGC 1 cut(s) 484
PleI GAGTC 2 cut(s) 257, 681
PpsI GAGTC 2 cut(s) 257, 681
Psp6I CCWGG 1 cut(s) 161
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 5 cut(s) 667, 696, 987, 1029, 1125
PspOMI GGGCCC 1 cut(s) 985
PspPI GGNCC 4 cut(s) 226, 255, 985, 986
PstNI CAGNNNCTG 1 cut(s) 223
PsuI RGATCY 3 cut(s) 614, 859, 1027
PvuII CAGCTG 1 cut(s) 929
RsaI GTAC 2 cut(s) 442, 774
RsaNI GTAC 2 cut(s) 441, 773
RseI CAYNNNNRTG 2 cut(s) 269, 432
Rsr2I CGGWCCG 1 cut(s) 255
RsrII CGGWCCG 1 cut(s) 255
SaqAI TTAA 3 cut(s) 188, 690, 1195
SatI GCNGC 1 cut(s) 483
Sau3AI GATC 8 cut(s) 3, 286, 307, 397, 614, 859, 917, 1027
Sau96I GGNCC 4 cut(s) 226, 255, 985, 986
ScaI AGTACT 2 cut(s) 442, 774
SchI GAGTC 2 cut(s) 258, 682
ScrFI CCNGG 3 cut(s) 163, 519, 989
SduI GDGCHC 2 cut(s) 989, 1128
SfaNI GCATC 5 cut(s) 283, 327, 494, 747, 1070
SfcI CTRYAG 1 cut(s) 853
SinI GGWCC 2 cut(s) 226, 255
SmiMI CAYNNNNRTG 2 cut(s) 269, 432
SmlI CTYRAG 2 cut(s) 677, 689
SmoI CTYRAG 2 cut(s) 677, 689
Sse9I AATT 7 cut(s) 346, 477, 569, 741, 996, 1137, 1192
SseBI AGGCCT 1 cut(s) 630
SsiI CCGC 4 cut(s) 159, 258, 746, 827
SspMI CTAG 1 cut(s) 1065
StuI AGGCCT 1 cut(s) 630
StyD4I CCNGG 3 cut(s) 161, 517, 987
StyI CCWWGG 2 cut(s) 427, 657
TaaI ACNGT 3 cut(s) 171, 594, 1147
TaiI ACGT 2 cut(s) 273, 756
TaqI TCGA 5 cut(s) 241, 267, 400, 588, 960
TaqII GACCGA 1 cut(s) 515
TasI AATT 7 cut(s) 346, 477, 569, 741, 996, 1137, 1192
TatI WGTACW 2 cut(s) 440, 772
TfiI GAWTC 4 cut(s) 220, 402, 653, 1175
Tru1I TTAA 3 cut(s) 188, 690, 1195
Tru9I TTAA 3 cut(s) 188, 690, 1195
TscAI CASTG 2 cut(s) 1112, 1150
TseFI GTSAC 2 cut(s) 9, 1021
TseI GCWGC 1 cut(s) 482
Tsp45I GTSAC 2 cut(s) 9, 1021
TspDTI ATGAA 3 cut(s) 253, 582, 992
TspGWI ACGGA 3 cut(s) 36, 194, 1040
TspRI CASTG 2 cut(s) 1112, 1150
Van91I CCANNNNNTGG 1 cut(s) 392
Vha464I CTTAAG 1 cut(s) 689
VpaK11BI GGWCC 2 cut(s) 226, 255
XapI RAATTY 2 cut(s) 346, 1137
XceI RCATGY 1 cut(s) 916
XmnI GAANNNNTTC 2 cut(s) 346, 821
XspI CTAG 1 cut(s) 1065
ZrmI AGTACT 2 cut(s) 442, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.