Rroxscaffold_5G00376250

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001162
Physical Location & Seq
Forward (+)
56979181 .. 56981478
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376250.1

Sequence Viewer

Length: 1194 bp
ATGAACCTCTCCCTGGGTTTCCGATCACCAGAGGCGTCTCTGTTTCCCCGGAGATATAGCTCTCCGATCAAACATCTGCAAAAACAGCAAGTGCCCAATTTACTCTCGTTCCCCAAACTACAATTTCCAGTGCCCAAAACTGGGTTCAGGGTCACGGCTCGGAAAGGCTCAGCTATAGAAGGCTTAAGCCAAGAGATGAACACCATCGCCTCGCAGAACCTGGATCACGCGCCTGCTCGGAGACGAGTTCGCTCGGCATTCATCGACGTGCATAAACAACTTGACCATTGCTTGTTCAAGATGGATCATGCTGGCATCAGAACAGAGGAGTGGTATGGAAGGAATTCAAGGGGTATGGAAATTTTCTGCAAAAGCTGGTTGCCAAAACCAGGTGATCAAATCAAAGCTGGCTTATGTTTTTGCCATGGGTACGGTAGTACTTGCACATTCTTCTTTGAAGGTATTGCTAGGAGAATTGCTCAGTCTGGGTACGCTGTATATGCAGTAGACTATCCAGGTTTTGGTCTTTCTGAAGGGTTGCATGGTTTCATCCCAGACTTTGATGAGTTGGTCGATGATGTTATTGAACAATTTACTAGTATCAAAGGAAGATCGGAAGTGGAAGGTTTGCCCTTTTTTATAATGGGGGAGTCGATGGGTGGAGCTGTCACTCTAAAGATTCATCTAAAGGAACCGCATGAATGGGATGGAGTGATCCTTGTAGCTCCAATGTGTAAAATTGCAGATGATGTGATGCCTCCAGCAATAGTGCTGAAGCTATTAGCCTTCATGTCTGAAGTTTTGCCTCGGGCAAAACTCTTCCCACAGAGAGATATAGCTCATCTAAGCTACAGAGAGCTCGGAAAGAGAAAAACGGCTGGTTACAATGTGATTTCTTATAAGGACCAAATGCGATCAAGAACAGCTGTAGAACTTCTGAAGGCCACTAGCGATATTGAAATGCAGTTGGATAAGGTTTCATCCCCATTGCTAATTCTTCATGGAGAAGCAGATAAGGTGACAGACCCTTTGGTGAGCCAGTTGCTGTATGAAAAGTCCTCTAGCAAGGATAAGACTTTGAAGCTCTATCAAGATGGTTATCACTGCATCCTTCAAGGGGAGCCTGATGACACAATTTTCACCGTCCTTGATGACATTGTTACTTGGCTTGATTCCCGGTGCTTCCGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

397

Amino Acids

44.78

Weight (kDa)

7.62

Isoelectric Point (pI)

48.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 133 - 375 2.8e-61 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 138 - 371 2.7e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 140 - 377 2.6e-13 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 641, 900
AccB7I CCANNNNNTGG 1 cut(s) 521
AccI GTMKAC 1 cut(s) 507
AccII CGCG 1 cut(s) 230
AciI CCGC 1 cut(s) 695
AclWI GGATC 3 cut(s) 231, 312, 709
AcsI RAATTY 2 cut(s) 343, 360
AcuI CTGAAG 4 cut(s) 552, 794, 816, 959
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 3 cut(s) 431, 439, 491
AfiI CCNNNNNNNGG 6 cut(s) 13, 140, 141, 389, 521, 1117
AflII CTTAAG 1 cut(s) 184
AgsI TTSAA 7 cut(s) 298, 348, 458, 587, 959, 1081, 1115
AhlI ACTAGT 1 cut(s) 596
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 4 cut(s) 12, 219, 388, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 2 cut(s) 42, 235
AlwI GGATC 3 cut(s) 231, 312, 709
AlwNI CAGNNNCTG 2 cut(s) 220, 1045
Ama87I CYCGRG 1 cut(s) 807
AoxI GGCC 1 cut(s) 942
ApoI RAATTY 2 cut(s) 343, 360
ArsI GACNNNNNNTTYG 2 cut(s) 1012, 1044
Asp700I GAANNNNTTC 1 cut(s) 343
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 904
AsuC2I CCSGG 2 cut(s) 49, 1177
AsuHPI GGTGA 5 cut(s) 18, 404, 1030, 1045, 1132
AvaI CYCGRG 1 cut(s) 807
AvaII GGWCC 1 cut(s) 904
BaeGI GKGCMC 2 cut(s) 96, 135
BaeI ACNNNNGTAYC 2 cut(s) 421, 454
BanII GRGCYC 1 cut(s) 861
Bbv12I GWGCWC 1 cut(s) 861
BccI CCATC 5 cut(s) 212, 295, 649, 701, 1088
BceAI ACGGC 2 cut(s) 171, 891
BciT130I CCWGG 4 cut(s) 14, 221, 390, 516
BclI TGATCA 1 cut(s) 394
BcnI CCSGG 2 cut(s) 49, 1177
BcoDI GTCTC 2 cut(s) 42, 235
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 5 cut(s) 468, 597, 948, 1062, 1192
BfmI CTRYAG 3 cut(s) 174, 850, 927
BfrI CTTAAG 1 cut(s) 184
BlpI GCTNAGC 1 cut(s) 169
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 6 cut(s) 14, 49, 221, 390, 516, 1177
Bme18I GGWCC 1 cut(s) 904
BmeT110I CYCGRG 1 cut(s) 807
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 904
BmiI GGNNCC 2 cut(s) 693, 1122
BmrFI CCNGG 6 cut(s) 14, 49, 221, 390, 516, 1177
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 3 cut(s) 324, 744, 1116
BmuI ACTGGG 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 169
BpuMI CCSGG 2 cut(s) 49, 1177
BsaBI GATNNNNATC 1 cut(s) 1098
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 5 cut(s) 12, 13, 47, 424, 806
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 140, 141, 389, 521, 1117
Bse1I ACTGG 3 cut(s) 128, 145, 1039
Bse3DI GCAATG 2 cut(s) 286, 986
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 4 cut(s) 14, 221, 390, 516
BseDI CCNNGG 5 cut(s) 12, 13, 47, 424, 806
BseGI GGATG 4 cut(s) 549, 712, 980, 1107
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 6 cut(s) 13, 140, 141, 389, 521, 1117
BseMI GCAATG 2 cut(s) 286, 986
BseMII CTCAG 2 cut(s) 183, 494
BseNI ACTGG 3 cut(s) 128, 145, 1039
BseRI GAGGAG 1 cut(s) 341
BseSI GKGCMC 2 cut(s) 96, 135
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 1 cut(s) 944
BsiHKAI GWGCWC 1 cut(s) 861
BsiHKCI CYCGRG 1 cut(s) 807
BsiSI CCGG 2 cut(s) 49, 1177
BslI CCNNNNNNNGG 6 cut(s) 13, 140, 141, 389, 521, 1117
BsmAI GTCTC 2 cut(s) 42, 235
BsmBI CGTCTC 2 cut(s) 42, 235
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 1 cut(s) 944
BsoBI CYCGRG 1 cut(s) 807
Bsp1286I GDGCHC 3 cut(s) 96, 135, 861
Bsp143I GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
Bsp1720I GCTNAGC 1 cut(s) 169
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 1 cut(s) 695
BspANI GGCC 1 cut(s) 944
BspCNI CTCAG 2 cut(s) 182, 493
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 2 cut(s) 693, 1122
BspPI GGATC 3 cut(s) 231, 312, 709
BspTI CTTAAG 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 286, 986
BsrI ACTGG 3 cut(s) 128, 145, 1039
BssECI CCNNGG 5 cut(s) 12, 13, 47, 424, 806
BssMI GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 4 cut(s) 14, 221, 390, 516
Bst4CI ACNGT 2 cut(s) 434, 1144
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 35
BstAFI CTTAAG 1 cut(s) 184
BstC8I GCNNGC 3 cut(s) 234, 313, 409
BstDEI CTNAG 3 cut(s) 169, 480, 845
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 4 cut(s) 549, 712, 980, 1107
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 8 cut(s) 26, 69, 226, 307, 397, 614, 717, 917
BstMAI GTCTC 2 cut(s) 42, 235
BstMBI GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
BstMWI GCNNNNNNNGC 2 cut(s) 85, 500
BstNI CCWGG 4 cut(s) 14, 221, 390, 516
BstSCI CCNGG 6 cut(s) 12, 47, 219, 388, 514, 1175
BstSFI CTRYAG 3 cut(s) 174, 850, 927
BstSLI GKGCMC 2 cut(s) 96, 135
BstUI CGCG 1 cut(s) 230
BsuRI GGCC 1 cut(s) 944
BtgI CCRYGG 1 cut(s) 424
BtgZI GCGATG 1 cut(s) 190
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 4 cut(s) 549, 712, 980, 1107
BtsI GCAGTG 1 cut(s) 1102
BtsIMutI CAGTG 2 cut(s) 135, 1102
Cac8I GCNNGC 3 cut(s) 234, 313, 409
CaiI CAGNNNCTG 2 cut(s) 220, 1045
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 904
CseI GACGC 1 cut(s) 24
CsiI ACCWGGT 1 cut(s) 388
Csp6I GTAC 3 cut(s) 430, 438, 490
CviAII CATG 6 cut(s) 308, 425, 542, 698, 790, 1001
CviQI GTAC 3 cut(s) 430, 438, 490
DdeI CTNAG 3 cut(s) 169, 480, 845
DpnI GATC 8 cut(s) 25, 68, 225, 306, 396, 613, 716, 916
DpnII GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 861
Eco47I GGWCC 1 cut(s) 904
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 4 cut(s) 552, 794, 816, 959
Eco88I CYCGRG 1 cut(s) 807
EcoICRI GAGCTC 1 cut(s) 859
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 4 cut(s) 12, 219, 388, 514
EcoT14I CCWWGG 1 cut(s) 424
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 2 cut(s) 42, 235
FaeI CATG 6 cut(s) 311, 428, 545, 701, 793, 1004
FatI CATG 6 cut(s) 307, 424, 541, 697, 789, 1000
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 507
FokI GGATG 4 cut(s) 536, 719, 967, 1094
FriOI GRGCYC 1 cut(s) 861
FspBI CTAG 5 cut(s) 468, 597, 948, 1062, 1192
GlaI GCGC 1 cut(s) 231
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 1 cut(s) 944
HapII CCGG 2 cut(s) 49, 1177
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 232
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 6 cut(s) 311, 428, 545, 701, 793, 1004
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 3 cut(s) 650, 679, 1172
HpaII CCGG 2 cut(s) 49, 1177
HphI GGTGA 5 cut(s) 18, 404, 1030, 1045, 1132
Hpy166II GTNNAC 1 cut(s) 508
Hpy188III TCNNGA 3 cut(s) 298, 918, 1091
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 8 cut(s) 173, 333, 452, 527, 617, 796, 934, 1121
HpyCH4III ACNGT 2 cut(s) 434, 1144
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 9 cut(s) 79, 271, 369, 444, 503, 541, 743, 964, 1107
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 500
HpyF3I CTNAG 3 cut(s) 169, 480, 845
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 6 cut(s) 311, 428, 545, 701, 793, 1004
HspAI GCGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
LmnI GCTCC 3 cut(s) 662, 730, 1120
LweI GCATC 3 cut(s) 324, 744, 1116
MabI ACCWGGT 1 cut(s) 388
MaeI CTAG 5 cut(s) 468, 597, 948, 1062, 1192
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 5 cut(s) 151, 667, 881, 1018, 1159
MalI GATC 8 cut(s) 25, 68, 225, 306, 396, 613, 716, 916
MboI GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
MboII GAAGA 4 cut(s) 442, 621, 811, 989
MhlI GDGCHC 3 cut(s) 96, 135, 861
MluCI AATT 9 cut(s) 97, 122, 343, 360, 474, 590, 738, 993, 1134
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 1 cut(s) 948
MnlI CCTC 7 cut(s) 17, 25, 220, 319, 768, 816, 1069
MroXI GAANNNNTTC 1 cut(s) 343
MseI TTAA 1 cut(s) 185
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 926
MspCI CTTAAG 1 cut(s) 184
MspI CCGG 2 cut(s) 49, 1177
MspR9I CCNGG 6 cut(s) 14, 49, 221, 390, 516, 1177
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 4 cut(s) 14, 221, 390, 516
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 2 cut(s) 85, 500
NciI CCSGG 2 cut(s) 49, 1177
NcoI CCATGG 1 cut(s) 424
NdeII GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
NlaIII CATG 6 cut(s) 311, 428, 545, 701, 793, 1004
NlaIV GGNNCC 2 cut(s) 693, 1122
NmeAIII GCCGAG 1 cut(s) 233
NmuCI GTSAC 3 cut(s) 151, 667, 1018
PasI CCCWGGG 1 cut(s) 13
PctI GAATGC 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 343
PfeI GAWTC 2 cut(s) 679, 1172
PflMI CCANNNNNTGG 1 cut(s) 521
PleI GAGTC 1 cut(s) 658
PpsI GAGTC 1 cut(s) 658
PsiI TTATAA 2 cut(s) 641, 900
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 4 cut(s) 12, 219, 388, 514
PspGI CCWGG 4 cut(s) 12, 219, 388, 514
PspN4I GGNNCC 2 cut(s) 693, 1122
PspPI GGNCC 1 cut(s) 904
PstNI CAGNNNCTG 2 cut(s) 220, 1045
PvuII CAGCTG 1 cut(s) 926
RsaI GTAC 3 cut(s) 431, 439, 491
RsaNI GTAC 3 cut(s) 430, 438, 490
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 861
SaqAI TTAA 1 cut(s) 185
Sau3AI GATC 8 cut(s) 23, 66, 223, 304, 394, 611, 714, 914
Sau96I GGNCC 1 cut(s) 904
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 6 cut(s) 14, 49, 221, 390, 516, 1177
SduI GDGCHC 3 cut(s) 96, 135, 861
SexAI ACCWGGT 1 cut(s) 388
SfaNI GCATC 3 cut(s) 324, 744, 1116
SfcI CTRYAG 3 cut(s) 174, 850, 927
SinI GGWCC 1 cut(s) 904
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 184
SmoI CTYRAG 1 cut(s) 184
SpeI ACTAGT 1 cut(s) 596
Sse9I AATT 9 cut(s) 97, 122, 343, 360, 474, 590, 738, 993, 1134
SsiI CCGC 1 cut(s) 695
SspMI CTAG 5 cut(s) 468, 597, 948, 1062, 1192
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 6 cut(s) 12, 47, 219, 388, 514, 1175
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 2 cut(s) 434, 1144
TaiI ACGT 1 cut(s) 270
TaqI TCGA 3 cut(s) 264, 573, 653
TasI AATT 9 cut(s) 97, 122, 343, 360, 474, 590, 738, 993, 1134
TatI WGTACW 1 cut(s) 437
TfiI GAWTC 2 cut(s) 679, 1172
Tru1I TTAA 1 cut(s) 185
Tru9I TTAA 1 cut(s) 185
TscAI CASTG 2 cut(s) 135, 1109
TseFI GTSAC 3 cut(s) 151, 667, 1018
Tsp45I GTSAC 3 cut(s) 151, 667, 1018
TspRI CASTG 2 cut(s) 135, 1109
Van91I CCANNNNNTGG 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 904
XapI RAATTY 2 cut(s) 343, 360
XmiI GTMKAC 1 cut(s) 507
XmnI GAANNNNTTC 1 cut(s) 343
XspI CTAG 5 cut(s) 468, 597, 948, 1062, 1192
ZrmI AGTACT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.