Rh4DG340000

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001162
Physical Location & Seq
Forward (+)
56242218 .. 56244707
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG340000.1

Sequence Viewer

Length: 1281 bp
ATGTCCACATTGGACGGTCAAAACTCTAGCATCAACCTTAGTCATCTGACTCAGTTTCATTCGAACTCTGAGAGCTCCGTGCTTAACGTTTTCCTCACCAAACTCACCATGGAACTTTCTCGAGCTCTGAGATTCCAGCAACTGGAGCTTCCTTTCTGCCCCCAACAACTCCAAACCCAGAGGCCTACATTTTCAGTCGCCGCCAGAAGAACAAGTCAGATAATAGTTGCAGCGGTCAAGACCAAGAGAAGAAGGCCACCCATTGAAGGCGTTAGCGAGGAGCTCAATTTGATCGCCGATCAGAGCCTTGACTGTGCTCCGGCTCGGAGAAGAGTTCGATCCGCATTCGTTGAGCTGCAGCAGCAACTTGATCACTGCTTGTTTAAGTTGGCTCCAACTGGGATCAGAACAGAAGAGTGGTACGAAATAAATTCAAGGGGGCTAGGAATTTTCTGTAAAAGCTGGAAGCCCAGAGAAGGGGTTCCGATTAAAGGCGTATTGTGTTTCTGCCATGGTTATGGTGATACTTGCGTTTTCTTCTTTGAAGGTATTGCCAAGCAGATTGCGGCAGCTGGGTACGCTGTGTACGCCATGGACTACCCAGGCTTTGGCCTTTCAGAAGGATTGCATGGTTATATCCCGAGCTTTGATCAGCTAGCTGACGATGTTATTGAACAGTATACAAAGATTAAAGCAAAACCTGAGTTGAAGGGGTTGCCTCACTTCATATTGGGCCAGTCCATGGGTGGAGCTGTTACTCTCAAAGTTCACTTTAAGCAACCATATGCATGGGATGGCATAGTTCTTGTGGCTCCAATGTGTAAAATTGCAGAGGATGTAACACCTCCACCAGCGGCAGCGAAGCTATTAACTCTTATGTCCAATGTTGTCCCCAAAGCAAAGCTTGTCCCGCAGAAAGACTTGGCCGAGCTGGCCTTCAGAGATGAAAGGAAAAGGAAACTGGCTGTCTACAATGTGACATGCTACAATGGCCCCGCGCGATTGAAGACTGCTGTGGAACTACTAAACGCTACTAAGGAGATTGAGATGCAAGTGCATAAGGTTTCATCTCCACTGCTAGTTCTTCATGGAGCTGCAGATAAGGTGACTGATCCCTTAGTGAGCCAGTTTCTTTATGAGAAGGCCTCAAGCAAAGACAAAACTCTAAAACTCTACCCAGATGGCTTTCATTGCATTCTTGAAGGAGAACCTGATGATAGAATCTTTTCAGTCCTTGAAGATATTATCACTTGGCTTGATCACCGGTGTTTGCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

426

Amino Acids

47.62

Weight (kDa)

8.52

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 163 - 404 4.4e-66 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 166 - 403 2e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 169 - 407 7.3e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 142, 608, 742
AccI GTMKAC 2 cut(s) 680, 969
AccII CGCG 2 cut(s) 998, 1000
AciI CCGC 7 cut(s) 201, 233, 342, 566, 854, 911, 996
AclI AACGTT 1 cut(s) 87
AclWI GGATC 3 cut(s) 333, 410, 1106
AcoI YGGCCR 1 cut(s) 924
AcsI RAATTY 2 cut(s) 430, 447
AcuI CTGAAG 1 cut(s) 922
AfaI GTAC 3 cut(s) 422, 578, 587
AfiI CCNNNNNNNGG 7 cut(s) 142, 266, 476, 477, 491, 608, 742
AgeI ACCGGT 1 cut(s) 1263
AgsI TTSAA 8 cut(s) 266, 435, 545, 674, 709, 1006, 1202, 1238
AjnI CCWGG 1 cut(s) 601
Alw21I GWGCWC 4 cut(s) 77, 127, 285, 319
AlwI GGATC 3 cut(s) 333, 410, 1106
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 2 cut(s) 120, 640
AoxI GGCC 8 cut(s) 182, 254, 610, 733, 924, 933, 991, 1143
ApeKI GCWGC 7 cut(s) 230, 355, 358, 361, 569, 857, 1094
ApoI RAATTY 2 cut(s) 430, 447
AsiGI ACCGGT 1 cut(s) 1263
Asp700I GAANNNNTTC 2 cut(s) 480, 1225
AspLEI GCGC 1 cut(s) 1000
AspS9I GGNCC 2 cut(s) 733, 992
AsuHPI GGTGA 5 cut(s) 88, 97, 533, 1117, 1253
AsuII TTCGAA 1 cut(s) 62
AsuNHI GCTAGC 1 cut(s) 655
AvaI CYCGRG 2 cut(s) 120, 640
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BanII GRGCYC 3 cut(s) 77, 127, 285
BbsI GAAGAC 1 cut(s) 1013
Bbv12I GWGCWC 4 cut(s) 77, 127, 285, 319
BbvI GCAGC 7 cut(s) 242, 342, 370, 373, 581, 869, 1081
BccI CCATC 2 cut(s) 788, 1175
BciT130I CCWGG 1 cut(s) 603
BclI TGATCA 3 cut(s) 370, 649, 1258
BfaI CTAG 4 cut(s) 27, 443, 656, 1079
BfmI CTRYAG 2 cut(s) 356, 1095
BglI GCCNNNNNGGC 1 cut(s) 932
Bme1390I CCNGG 1 cut(s) 603
BmeT110I CYCGRG 2 cut(s) 120, 640
BmgT120I GGNCC 2 cut(s) 733, 992
BmiI GGNNCC 4 cut(s) 393, 483, 813, 994
BmrFI CCNGG 1 cut(s) 603
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 2 cut(s) 39, 1038
BmtI GCTAGC 1 cut(s) 659
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 1013
BplI GAGNNNNNCTC 2 cut(s) 1130, 1162
BpmI CTGGAG 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 62
BpuEI CTTGAG 2 cut(s) 1132, 1259
BsaJI CCNNGG 5 cut(s) 108, 511, 591, 601, 741
BsaWI WCCGGW 1 cut(s) 1263
Bsc4I CCNNNNNNNGG 7 cut(s) 142, 266, 476, 477, 491, 608, 742
Bse118I RCCGGY 1 cut(s) 1263
Bse1I ACTGG 5 cut(s) 147, 403, 736, 966, 1126
Bse3DI GCAATG 1 cut(s) 1189
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 5 cut(s) 108, 511, 591, 601, 741
BseGI GGATG 2 cut(s) 799, 841
BseLI CCNNNNNNNGG 7 cut(s) 142, 266, 476, 477, 491, 608, 742
BseMI GCAATG 1 cut(s) 1189
BseMII CTCAG 4 cut(s) 60, 65, 119, 693
BseNI ACTGG 5 cut(s) 147, 403, 736, 966, 1126
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 7 cut(s) 242, 342, 370, 373, 581, 869, 1081
BseYI CCCAGC 1 cut(s) 572
Bsh1236I CGCG 2 cut(s) 998, 1000
BshFI GGCC 8 cut(s) 184, 256, 612, 735, 926, 935, 993, 1145
BshTI ACCGGT 1 cut(s) 1263
BsiHKAI GWGCWC 4 cut(s) 77, 127, 285, 319
BsiHKCI CYCGRG 2 cut(s) 120, 640
BsiSI CCGG 2 cut(s) 320, 1264
BslFI GGGAC 2 cut(s) 875, 893
BslI CCNNNNNNNGG 7 cut(s) 142, 266, 476, 477, 491, 608, 742
BsmFI GGGAC 2 cut(s) 875, 893
BsmI GAATGC 2 cut(s) 344, 1194
BsnI GGCC 8 cut(s) 184, 256, 612, 735, 926, 935, 993, 1145
BsoBI CYCGRG 2 cut(s) 120, 640
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 4 cut(s) 77, 127, 285, 319
Bsp143I GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
Bsp19I CCATGG 4 cut(s) 108, 511, 591, 741
BspACI CCGC 7 cut(s) 201, 233, 342, 566, 854, 911, 996
BspANI GGCC 8 cut(s) 184, 256, 612, 735, 926, 935, 993, 1145
BspCNI CTCAG 4 cut(s) 61, 64, 120, 694
BspFNI CGCG 2 cut(s) 998, 1000
BspLI GGNNCC 4 cut(s) 393, 483, 813, 994
BspMAI CTGCAG 2 cut(s) 360, 1099
BspOI GCTAGC 1 cut(s) 659
BspPI GGATC 3 cut(s) 333, 410, 1106
BspT104I TTCGAA 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 1189
BsrFI RCCGGY 1 cut(s) 1263
BsrI ACTGG 5 cut(s) 147, 403, 736, 966, 1126
BssAI RCCGGY 1 cut(s) 1263
BssECI CCNNGG 5 cut(s) 108, 511, 591, 601, 741
BssMI GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
BssNAI GTATAC 1 cut(s) 681
BssT1I CCWWGG 4 cut(s) 108, 511, 591, 741
Bst1107I GTATAC 1 cut(s) 681
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 3 cut(s) 17, 314, 678
Bst6I CTCTTC 2 cut(s) 325, 408
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 657, 933
BstDEI CTNAG 7 cut(s) 38, 51, 69, 128, 702, 1035, 1117
BstDSI CCRYGG 4 cut(s) 108, 511, 591, 741
BstF5I GGATG 2 cut(s) 799, 841
BstFNI CGCG 2 cut(s) 998, 1000
BstHHI GCGC 1 cut(s) 1000
BstKTI GATC 8 cut(s) 294, 301, 341, 373, 405, 652, 1114, 1261
BstMBI GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
BstMWI GCNNNNNNNGC 8 cut(s) 145, 361, 578, 587, 910, 932, 990, 1191
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 1 cut(s) 984
BstSCI CCNGG 1 cut(s) 601
BstSFI CTRYAG 2 cut(s) 356, 1095
BstUI CGCG 2 cut(s) 998, 1000
BstV1I GCAGC 7 cut(s) 242, 342, 370, 373, 581, 869, 1081
BstV2I GAAGAC 1 cut(s) 1013
BstXI CCANNNNNNTGG 2 cut(s) 518, 789
BstZ17I GTATAC 1 cut(s) 681
BsuRI GGCC 8 cut(s) 184, 256, 612, 735, 926, 935, 993, 1145
BtgI CCRYGG 4 cut(s) 108, 511, 591, 741
BtsCI GGATG 2 cut(s) 799, 841
BtsI GCAGTG 2 cut(s) 373, 1073
BtsIMutI CAGTG 2 cut(s) 373, 1073
Cac8I GCNNGC 2 cut(s) 657, 933
CaiI CAGNNNCTG 1 cut(s) 142
CfoI GCGC 1 cut(s) 1000
Cfr10I RCCGGY 1 cut(s) 1263
Cfr13I GGNCC 2 cut(s) 733, 992
Csp6I GTAC 3 cut(s) 421, 577, 586
CspAI ACCGGT 1 cut(s) 1263
CviAII CATG 8 cut(s) 109, 512, 592, 629, 742, 789, 981, 1088
CviQI GTAC 3 cut(s) 421, 577, 586
DdeI CTNAG 7 cut(s) 38, 51, 69, 128, 702, 1035, 1117
DpnI GATC 8 cut(s) 293, 300, 340, 372, 404, 651, 1113, 1260
DpnII GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
EaeI YGGCCR 1 cut(s) 924
Eam1104I CTCTTC 2 cut(s) 325, 408
EarI CTCTTC 2 cut(s) 325, 408
Ecl136II GAGCTC 3 cut(s) 75, 125, 283
Eco130I CCWWGG 4 cut(s) 108, 511, 591, 741
Eco147I AGGCCT 2 cut(s) 184, 1145
Eco24I GRGCYC 3 cut(s) 77, 127, 285
Eco53kI GAGCTC 3 cut(s) 75, 125, 283
Eco57I CTGAAG 1 cut(s) 922
Eco88I CYCGRG 2 cut(s) 120, 640
EcoICRI GAGCTC 3 cut(s) 75, 125, 283
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 4 cut(s) 108, 511, 591, 741
EcoT22I ATGCAT 1 cut(s) 790
EcoT38I GRGCYC 3 cut(s) 77, 127, 285
ErhI CCWWGG 4 cut(s) 108, 511, 591, 741
FaeI CATG 8 cut(s) 112, 515, 595, 632, 745, 792, 984, 1091
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 2 cut(s) 875, 893
FatI CATG 8 cut(s) 108, 511, 591, 628, 741, 788, 980, 1087
FauI CCCGC 2 cut(s) 918, 1003
FauNDI CATATG 1 cut(s) 784
FbaI TGATCA 3 cut(s) 370, 649, 1258
FblI GTMKAC 2 cut(s) 680, 969
FokI GGATG 2 cut(s) 806, 848
FriOI GRGCYC 3 cut(s) 77, 127, 285
FspBI CTAG 4 cut(s) 27, 443, 656, 1079
GlaI GCGC 1 cut(s) 999
GsaI CCCAGC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 164
HaeIII GGCC 8 cut(s) 184, 256, 612, 735, 926, 935, 993, 1145
HapII CCGG 2 cut(s) 320, 1264
HhaI GCGC 1 cut(s) 1000
Hin1II CATG 8 cut(s) 112, 515, 595, 632, 745, 792, 984, 1091
Hin6I GCGC 1 cut(s) 998
HinP1I GCGC 1 cut(s) 998
HindIII AAGCTT 1 cut(s) 902
HinfI GANTC 3 cut(s) 49, 132, 1221
HpaII CCGG 2 cut(s) 320, 1264
HphI GGTGA 5 cut(s) 88, 97, 533, 1117, 1253
Hpy166II GTNNAC 5 cut(s) 6, 586, 681, 769, 970
Hpy188III TCNNGA 4 cut(s) 120, 238, 640, 1199
Hpy8I GTNNAC 5 cut(s) 6, 586, 681, 769, 970
HpyAV CCTTC 9 cut(s) 246, 260, 470, 539, 614, 703, 946, 1135, 1196
HpyCH4III ACNGT 3 cut(s) 17, 314, 678
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 9 cut(s) 230, 358, 628, 788, 830, 1051, 1057, 1097, 1194
HpyF10VI GCNNNNNNNGC 8 cut(s) 145, 361, 578, 587, 910, 932, 990, 1191
HpyF3I CTNAG 7 cut(s) 38, 51, 69, 128, 702, 1035, 1117
HpySE526I ACGT 1 cut(s) 87
Hsp92II CATG 8 cut(s) 112, 515, 595, 632, 745, 792, 984, 1091
HspAI GCGC 1 cut(s) 998
Ksp22I TGATCA 3 cut(s) 370, 649, 1258
Kzo9I GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
LmnI GCTCC 8 cut(s) 80, 145, 280, 322, 397, 749, 817, 1091
Lsp1109I GCAGC 7 cut(s) 242, 342, 370, 373, 581, 869, 1081
LweI GCATC 2 cut(s) 39, 1038
MaeI CTAG 4 cut(s) 27, 443, 656, 1079
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 4 cut(s) 754, 838, 976, 1105
MalI GATC 8 cut(s) 293, 300, 340, 372, 404, 651, 1113, 1260
MboI GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
MboII GAAGA 8 cut(s) 219, 261, 342, 425, 529, 1018, 1076, 1250
MhlI GDGCHC 4 cut(s) 77, 127, 285, 319
MluCI AATT 4 cut(s) 286, 430, 447, 825
MlyI GAGTC 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 419
MnlI CCTC 7 cut(s) 104, 174, 271, 729, 826, 855, 1156
Mph1103I ATGCAT 1 cut(s) 790
MroXI GAANNNNTTC 2 cut(s) 480, 1225
MseI TTAA 6 cut(s) 84, 384, 489, 690, 774, 869
MslI CAYNNNNRTG 2 cut(s) 516, 787
MspA1I CMGCKG 3 cut(s) 233, 572, 854
MspI CCGG 2 cut(s) 320, 1264
MspR9I CCNGG 1 cut(s) 603
Mva1269I GAATGC 2 cut(s) 344, 1194
MvaI CCWGG 1 cut(s) 603
MvnI CGCG 2 cut(s) 998, 1000
MwoI GCNNNNNNNGC 8 cut(s) 145, 361, 578, 587, 910, 932, 990, 1191
NcoI CCATGG 4 cut(s) 108, 511, 591, 741
NdeI CATATG 1 cut(s) 784
NdeII GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
NheI GCTAGC 1 cut(s) 655
NlaIII CATG 8 cut(s) 112, 515, 595, 632, 745, 792, 984, 1091
NlaIV GGNNCC 4 cut(s) 393, 483, 813, 994
NmeAIII GCCGAG 1 cut(s) 952
NmuCI GTSAC 2 cut(s) 976, 1105
NsiI ATGCAT 1 cut(s) 790
NspI RCATGY 1 cut(s) 984
NspV TTCGAA 1 cut(s) 62
PaeR7I CTCGAG 1 cut(s) 120
PceI AGGCCT 2 cut(s) 184, 1145
PctI GAATGC 2 cut(s) 344, 1194
PdmI GAANNNNTTC 2 cut(s) 480, 1225
PfeI GAWTC 2 cut(s) 132, 1221
PflMI CCANNNNNTGG 3 cut(s) 142, 608, 742
PinAI ACCGGT 1 cut(s) 1263
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp124BI GAGCTC 3 cut(s) 77, 127, 285
Psp1406I AACGTT 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 601
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 4 cut(s) 393, 483, 813, 994
PspPI GGNCC 2 cut(s) 733, 992
PstI CTGCAG 2 cut(s) 360, 1099
PstNI CAGNNNCTG 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 3 cut(s) 422, 578, 587
RsaNI GTAC 3 cut(s) 421, 577, 586
RseI CAYNNNNRTG 2 cut(s) 516, 787
SacI GAGCTC 3 cut(s) 77, 127, 285
SaqAI TTAA 6 cut(s) 84, 384, 489, 690, 774, 869
Sau3AI GATC 8 cut(s) 291, 298, 338, 370, 402, 649, 1111, 1258
Sau96I GGNCC 2 cut(s) 733, 992
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 4 cut(s) 77, 127, 285, 319
SfaNI GCATC 2 cut(s) 39, 1038
SfcI CTRYAG 2 cut(s) 356, 1095
SfiI GGCCNNNNNGGCC 1 cut(s) 932
Sfr274I CTCGAG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 62
SgrAI CRCCGGYG 1 cut(s) 1263
SlaI CTCGAG 1 cut(s) 120
SmiMI CAYNNNNRTG 2 cut(s) 516, 787
SmlI CTYRAG 3 cut(s) 120, 1147, 1274
SmoI CTYRAG 3 cut(s) 120, 1147, 1274
Sse9I AATT 4 cut(s) 286, 430, 447, 825
SseBI AGGCCT 2 cut(s) 184, 1145
SsiI CCGC 7 cut(s) 201, 233, 342, 566, 854, 911, 996
SspMI CTAG 4 cut(s) 27, 443, 656, 1079
SstI GAGCTC 3 cut(s) 77, 127, 285
StuI AGGCCT 2 cut(s) 184, 1145
StyD4I CCNGG 1 cut(s) 601
StyI CCWWGG 4 cut(s) 108, 511, 591, 741
TaaI ACNGT 3 cut(s) 17, 314, 678
TaiI ACGT 1 cut(s) 90
TaqI TCGA 3 cut(s) 62, 121, 337
TasI AATT 4 cut(s) 286, 430, 447, 825
TauI GCSGC 3 cut(s) 203, 569, 857
TfiI GAWTC 2 cut(s) 132, 1221
Tru1I TTAA 6 cut(s) 84, 384, 489, 690, 774, 869
Tru9I TTAA 6 cut(s) 84, 384, 489, 690, 774, 869
TscAI CASTG 2 cut(s) 380, 1080
TseFI GTSAC 2 cut(s) 976, 1105
TseI GCWGC 7 cut(s) 230, 355, 358, 361, 569, 857, 1094
Tsp45I GTSAC 2 cut(s) 976, 1105
TspDTI ATGAA 6 cut(s) 47, 715, 960, 1056, 1076, 1178
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 2 cut(s) 380, 1080
Van91I CCANNNNNTGG 3 cut(s) 142, 608, 742
XapI RAATTY 2 cut(s) 430, 447
XceI RCATGY 1 cut(s) 984
XcmI CCANNNNNNNNNTGG 2 cut(s) 106, 743
XhoI CTCGAG 1 cut(s) 120
XmiI GTMKAC 2 cut(s) 680, 969
XmnI GAANNNNTTC 2 cut(s) 480, 1225
XspI CTAG 4 cut(s) 27, 443, 656, 1079
Zsp2I ATGCAT 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.