FvH4_4g27270

Caffeoylshikimate esterase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001162
Physical Location & Seq
Forward (+)
28329851 .. 28332832
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27270.t3

Sequence Viewer

Length: 1374 bp
ATGGAAAACCAAACAGACAATCCAGATAAAGAATTCGACACATGCTCCATGTGCATCTTCCAAAACCCAAAGAAAGCGACGTCCACTTTGGACGGTCAAAACCCATACCTCTACAACAAAACCAGAACTATCTTCAACCTTAGTCTTCTGACTCAGTTTCATTCGAACTCTGAGAGCTCCGTGCTTAACTTTTTCCTCAACTCCACCATGGAACTTTCTCAATCTCTGAGATTCCAGCAACTGGAGCTTCCATTTTGCCCCCAACAACTCCAGCAACCCCAGAAGCCAACATTTTCAGTCAGAAGAACAAGTCAGGTAATAGTTGCAGCGGCCAAAACCAAGAGAAGGCCACCCATTGAAGGTGTTAGTGAGGAGCTCAATTTGATCGCCGATCAGAGCCTTGACTTTGCTCCGGCTAGGAGAAGAGTTCGATCCGCATTCGTTGAGCTGCAGCAACAGCTTGATCACTGCTTGTTTAAGTTGGCTCCAACTGGGATCAGAACAGAAGAGTGGTATGAAATAAATTCAAGGGGAATTGGAATTTTCTGTAAAAGCTGGAAGCCAAGAGAAGGTGTTCCGATTAAAGGGGTGTTGTGTTTCTGCCATGGGTATGGTGACACTTGCGTTTTCTTCTTTGAAGGTATTGCCAAGCAAATTGCTGCGGCTGGGTACGCTGTGTACGCCATGGACTACCCAGGATTTGGCCTTTCGGAAGGATTGCACGGTTTTATCCCGAGCTTTGATCAGTTAGCCGATGATGTTATTGAACAGTATACAAAGATTAAAGCAAAACCAGAGTTGAAAGGGTTGCCTCACTTCATATTGGGGCAGTCCATGGGTGGAGCTGTTACTCTAAAAGTTCACTTCAAGCAACCATATGCCTGGGATGGCATAGTTCTTGTGGCTCCAATGTGTAAAATTGCAGAGGATGTAACACCTCCACCTGCGGCACAGAAGATATTAACTCTTATGTCCAGAGTTGTCCCTAAAGCAAAGCTTGTCCCACAGAAAGACTTGGCCGAGTTGGCCTTCAGAGATGAAAGGAAGAGGAAACTGGCTGTCTACAATGTGACATGCTACAATGGCCCCGTGCGATTGAAGACTGCTGTGGAACTTCTAAACGCTACTAAGGAGATAGAGATGCAAGTGGATAAGGTTTCATCTCCACTGCTAATTCTTCATGGAGCTGCAGATAAGGTGACTGATCCCTTAGTGAGCCAGTTTCTTTATGAGAAGGCATCAAGCAAAGACAAAAGTCTAAAACTCTACCCTGATGGCTTTCATTGCATTCTTGAAGGTGAACCTGATGATAGAATTTTTTCAGTCCTTGAAGATATTATCACTTGGCTTGATCACCGGTGTTTGCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

458

Amino Acids

51.48

Weight (kDa)

7.89

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 194 - 307 5.4e-33 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 197 - 303 1.4e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 200 - 308 1.6e-08 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 952
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 1 cut(s) 952
AccB7I CCANNNNNTGG 2 cut(s) 241, 701
AccI GTMKAC 2 cut(s) 773, 1062
AciI CCGC 4 cut(s) 329, 435, 662, 947
AclWI GGATC 3 cut(s) 426, 503, 1199
AcoI YGGCCR 2 cut(s) 330, 1017
AcsI RAATTY 4 cut(s) 32, 523, 540, 1314
AcuI CTGAAG 1 cut(s) 1015
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 2 cut(s) 671, 680
AfiI CCNNNNNNNGG 6 cut(s) 241, 345, 359, 569, 584, 701
AgeI ACCGGT 1 cut(s) 1356
AjnI CCWGG 2 cut(s) 694, 881
Alw21I GWGCWC 2 cut(s) 179, 378
AlwI GGATC 3 cut(s) 426, 503, 1199
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 1 cut(s) 733
AoxI GGCC 6 cut(s) 330, 347, 703, 1017, 1026, 1084
ApeKI GCWGC 5 cut(s) 326, 448, 451, 659, 1187
ApoI RAATTY 4 cut(s) 32, 523, 540, 1314
ArsI GACNNNNNNTTYG 2 cut(s) 70, 102
AsiGI ACCGGT 1 cut(s) 1356
Asp700I GAANNNNTTC 2 cut(s) 573, 1318
AspS9I GGNCC 1 cut(s) 1085
AsuHPI GGTGA 4 cut(s) 626, 1210, 1310, 1346
AsuII TTCGAA 1 cut(s) 164
AvaI CYCGRG 1 cut(s) 733
BanII GRGCYC 2 cut(s) 179, 378
BbsI GAAGAC 2 cut(s) 137, 1106
Bbv12I GWGCWC 2 cut(s) 179, 378
BbvI GCAGC 5 cut(s) 338, 435, 463, 646, 1174
BccI CCATC 2 cut(s) 881, 1268
BciT130I CCWGG 2 cut(s) 696, 883
BclI TGATCA 3 cut(s) 463, 742, 1351
BfaI CTAG 1 cut(s) 417
BfmI CTRYAG 2 cut(s) 449, 1188
BfuAI ACCTGC 1 cut(s) 952
BglI GCCNNNNNGGC 1 cut(s) 1025
BisI GCNGC 8 cut(s) 327, 330, 449, 452, 660, 663, 948, 1188
BlsI GCNGC 8 cut(s) 328, 331, 450, 453, 661, 664, 949, 1189
Bme1390I CCNGG 2 cut(s) 696, 883
BmeT110I CYCGRG 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 1085
BmiI GGNNCC 3 cut(s) 486, 906, 1087
BmrFI CCNGG 2 cut(s) 696, 883
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 3 cut(s) 63, 1131, 1247
BmuI ACTGGG 1 cut(s) 501
BoxI GACNNNNGTC 1 cut(s) 1254
BpiI GAAGAC 2 cut(s) 137, 1106
BpmI CTGGAG 2 cut(s) 254, 263
Bpu14I TTCGAA 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 1352
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 6 cut(s) 207, 604, 684, 694, 834, 882
BsaWI WCCGGW 1 cut(s) 1356
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 6 cut(s) 241, 345, 359, 569, 584, 701
Bse118I RCCGGY 1 cut(s) 1356
Bse1I ACTGG 4 cut(s) 246, 496, 1059, 1219
Bse3DI GCAATG 1 cut(s) 1282
BseBI CCWGG 2 cut(s) 696, 883
BseDI CCNNGG 6 cut(s) 207, 604, 684, 694, 834, 882
BseGI GGATG 2 cut(s) 892, 934
BseLI CCNNNNNNNGG 6 cut(s) 241, 345, 359, 569, 584, 701
BseMI GCAATG 1 cut(s) 1282
BseMII CTCAG 3 cut(s) 162, 167, 218
BseNI ACTGG 4 cut(s) 246, 496, 1059, 1219
BseRI GAGGAG 1 cut(s) 386
BseXI GCAGC 5 cut(s) 338, 435, 463, 646, 1174
BseYI CCCAGC 1 cut(s) 665
BshFI GGCC 6 cut(s) 332, 349, 705, 1019, 1028, 1086
BshTI ACCGGT 1 cut(s) 1356
BsiHKAI GWGCWC 2 cut(s) 179, 378
BsiHKCI CYCGRG 1 cut(s) 733
BsiSI CCGG 2 cut(s) 413, 1357
BslFI GGGAC 2 cut(s) 968, 986
BslI CCNNNNNNNGG 6 cut(s) 241, 345, 359, 569, 584, 701
BsmFI GGGAC 2 cut(s) 968, 986
BsmI GAATGC 2 cut(s) 437, 1287
BsnI GGCC 6 cut(s) 332, 349, 705, 1019, 1028, 1086
BsoBI CYCGRG 1 cut(s) 733
Bsp119I TTCGAA 1 cut(s) 164
Bsp1286I GDGCHC 2 cut(s) 179, 378
Bsp143I GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
Bsp19I CCATGG 4 cut(s) 207, 604, 684, 834
BspACI CCGC 4 cut(s) 329, 435, 662, 947
BspANI GGCC 6 cut(s) 332, 349, 705, 1019, 1028, 1086
BspCNI CTCAG 3 cut(s) 163, 166, 219
BspLI GGNNCC 3 cut(s) 486, 906, 1087
BspMAI CTGCAG 2 cut(s) 453, 1192
BspMI ACCTGC 1 cut(s) 952
BspPI GGATC 3 cut(s) 426, 503, 1199
BspT104I TTCGAA 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 1282
BsrFI RCCGGY 1 cut(s) 1356
BsrI ACTGG 4 cut(s) 246, 496, 1059, 1219
BssAI RCCGGY 1 cut(s) 1356
BssECI CCNNGG 6 cut(s) 207, 604, 684, 694, 834, 882
BssMI GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
BssNAI GTATAC 1 cut(s) 774
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 4 cut(s) 207, 604, 684, 834
Bst1107I GTATAC 1 cut(s) 774
Bst2UI CCWGG 2 cut(s) 696, 883
Bst4CI ACNGT 3 cut(s) 95, 725, 771
Bst6I CTCTTC 3 cut(s) 418, 501, 1040
BstACI GRCGYC 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 164
BstDEI CTNAG 6 cut(s) 140, 153, 171, 227, 1128, 1210
BstDSI CCRYGG 4 cut(s) 207, 604, 684, 834
BstF5I GGATG 2 cut(s) 892, 934
BstKTI GATC 8 cut(s) 387, 394, 434, 466, 498, 745, 1207, 1354
BstMBI GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
BstMWI GCNNNNNNNGC 8 cut(s) 51, 244, 457, 671, 680, 1025, 1083, 1284
BstNI CCWGG 2 cut(s) 696, 883
BstNSI RCATGY 2 cut(s) 45, 1077
BstPAI GACNNNNGTC 1 cut(s) 1254
BstSCI CCNGG 2 cut(s) 694, 881
BstSFI CTRYAG 2 cut(s) 449, 1188
BstV1I GCAGC 5 cut(s) 338, 435, 463, 646, 1174
BstV2I GAAGAC 2 cut(s) 137, 1106
BstXI CCANNNNNNTGG 2 cut(s) 611, 882
BstZ17I GTATAC 1 cut(s) 774
BsuRI GGCC 6 cut(s) 332, 349, 705, 1019, 1028, 1086
BtgI CCRYGG 4 cut(s) 207, 604, 684, 834
BtsCI GGATG 2 cut(s) 892, 934
BtsI GCAGTG 2 cut(s) 466, 1166
BtsIMutI CAGTG 2 cut(s) 466, 1166
BveI ACCTGC 1 cut(s) 952
CaiI CAGNNNCTG 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 1356
Cfr13I GGNCC 1 cut(s) 1085
Csp6I GTAC 2 cut(s) 670, 679
CspAI ACCGGT 1 cut(s) 1356
CviAII CATG 8 cut(s) 42, 49, 208, 605, 685, 835, 1074, 1181
CviQI GTAC 2 cut(s) 670, 679
DdeI CTNAG 6 cut(s) 140, 153, 171, 227, 1128, 1210
DpnI GATC 8 cut(s) 386, 393, 433, 465, 497, 744, 1206, 1353
DpnII GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
EaeI YGGCCR 2 cut(s) 330, 1017
Eam1104I CTCTTC 3 cut(s) 418, 501, 1040
EarI CTCTTC 3 cut(s) 418, 501, 1040
Ecl136II GAGCTC 2 cut(s) 177, 376
Eco130I CCWWGG 4 cut(s) 207, 604, 684, 834
Eco24I GRGCYC 2 cut(s) 179, 378
Eco53kI GAGCTC 2 cut(s) 177, 376
Eco57I CTGAAG 1 cut(s) 1015
Eco88I CYCGRG 1 cut(s) 733
EcoICRI GAGCTC 2 cut(s) 177, 376
EcoRI GAATTC 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 694, 881
EcoT14I CCWWGG 4 cut(s) 207, 604, 684, 834
EcoT38I GRGCYC 2 cut(s) 179, 378
ErhI CCWWGG 4 cut(s) 207, 604, 684, 834
FaeI CATG 8 cut(s) 45, 52, 211, 608, 688, 838, 1077, 1184
FalI AAGNNNNNCTT 2 cut(s) 981, 1013
FaqI GGGAC 2 cut(s) 968, 986
FatI CATG 8 cut(s) 41, 48, 207, 604, 684, 834, 1073, 1180
FauNDI CATATG 1 cut(s) 877
FbaI TGATCA 3 cut(s) 463, 742, 1351
FblI GTMKAC 2 cut(s) 773, 1062
Fnu4HI GCNGC 8 cut(s) 327, 330, 449, 452, 660, 663, 948, 1188
FokI GGATG 2 cut(s) 899, 941
FriOI GRGCYC 2 cut(s) 179, 378
Fsp4HI GCNGC 8 cut(s) 327, 330, 449, 452, 660, 663, 948, 1188
FspBI CTAG 1 cut(s) 417
GluI GCNGC 8 cut(s) 327, 330, 449, 452, 660, 663, 948, 1188
GsaI CCCAGC 1 cut(s) 669
GsuI CTGGAG 2 cut(s) 254, 263
HaeIII GGCC 6 cut(s) 332, 349, 705, 1019, 1028, 1086
HapII CCGG 2 cut(s) 413, 1357
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 8 cut(s) 45, 52, 211, 608, 688, 838, 1077, 1184
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 2 cut(s) 151, 231
HpaII CCGG 2 cut(s) 413, 1357
HphI GGTGA 4 cut(s) 626, 1210, 1310, 1346
Hpy166II GTNNAC 6 cut(s) 84, 679, 774, 862, 1063, 1301
Hpy188I TCNGA 9 cut(s) 150, 172, 228, 302, 396, 500, 579, 712, 1034
Hpy188III TCNNGA 4 cut(s) 23, 733, 975, 1292
Hpy8I GTNNAC 6 cut(s) 84, 679, 774, 862, 1063, 1301
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 8 cut(s) 339, 353, 563, 632, 707, 1039, 1228, 1289
HpyCH4III ACNGT 3 cut(s) 95, 725, 771
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 8 cut(s) 54, 326, 451, 721, 923, 1144, 1190, 1287
HpyF10VI GCNNNNNNNGC 8 cut(s) 51, 244, 457, 671, 680, 1025, 1083, 1284
HpyF3I CTNAG 6 cut(s) 140, 153, 171, 227, 1128, 1210
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 8 cut(s) 45, 52, 211, 608, 688, 838, 1077, 1184
Ksp22I TGATCA 3 cut(s) 463, 742, 1351
Kzo9I GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
LmnI GCTCC 9 cut(s) 50, 182, 244, 373, 415, 490, 842, 910, 1184
Lsp1109I GCAGC 5 cut(s) 338, 435, 463, 646, 1174
LweI GCATC 3 cut(s) 63, 1131, 1247
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 5 cut(s) 614, 847, 931, 1069, 1198
MalI GATC 8 cut(s) 386, 393, 433, 465, 497, 744, 1206, 1353
MboI GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
MhlI GDGCHC 2 cut(s) 179, 378
MluCI AATT 9 cut(s) 32, 379, 523, 534, 540, 654, 918, 1173, 1314
MlyI GAGTC 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 512
MnlI CCTC 7 cut(s) 119, 206, 364, 822, 919, 948, 1041
MroXI GAANNNNTTC 2 cut(s) 573, 1318
MseI TTAA 5 cut(s) 186, 477, 582, 783, 962
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 2 cut(s) 413, 1357
MspR9I CCNGG 2 cut(s) 696, 883
Mva1269I GAATGC 2 cut(s) 437, 1287
MvaI CCWGG 2 cut(s) 696, 883
MwoI GCNNNNNNNGC 8 cut(s) 51, 244, 457, 671, 680, 1025, 1083, 1284
NcoI CCATGG 4 cut(s) 207, 604, 684, 834
NdeI CATATG 1 cut(s) 877
NdeII GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
NlaIII CATG 8 cut(s) 45, 52, 211, 608, 688, 838, 1077, 1184
NlaIV GGNNCC 3 cut(s) 486, 906, 1087
NmeAIII GCCGAG 1 cut(s) 1045
NmuCI GTSAC 3 cut(s) 614, 1069, 1198
NspI RCATGY 2 cut(s) 45, 1077
NspV TTCGAA 1 cut(s) 164
PaqCI CACCTGC 1 cut(s) 952
PctI GAATGC 2 cut(s) 437, 1287
PdmI GAANNNNTTC 2 cut(s) 573, 1318
PfeI GAWTC 1 cut(s) 231
PflMI CCANNNNNTGG 2 cut(s) 241, 701
PinAI ACCGGT 1 cut(s) 1356
PkrI GCNGC 8 cut(s) 328, 331, 450, 453, 661, 664, 949, 1189
PleI GAGTC 1 cut(s) 145
PpsI GAGTC 1 cut(s) 145
PshAI GACNNNNGTC 1 cut(s) 1254
Psp124BI GAGCTC 2 cut(s) 179, 378
Psp6I CCWGG 2 cut(s) 694, 881
PspFI CCCAGC 1 cut(s) 665
PspGI CCWGG 2 cut(s) 694, 881
PspN4I GGNNCC 3 cut(s) 486, 906, 1087
PspPI GGNCC 1 cut(s) 1085
PstI CTGCAG 2 cut(s) 453, 1192
PstNI CAGNNNCTG 1 cut(s) 241
RsaI GTAC 2 cut(s) 671, 680
RsaNI GTAC 2 cut(s) 670, 679
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 2 cut(s) 179, 378
SaqAI TTAA 5 cut(s) 186, 477, 582, 783, 962
SatI GCNGC 8 cut(s) 327, 330, 449, 452, 660, 663, 948, 1188
Sau3AI GATC 8 cut(s) 384, 391, 431, 463, 495, 742, 1204, 1351
Sau96I GGNCC 1 cut(s) 1085
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 2 cut(s) 696, 883
SduI GDGCHC 2 cut(s) 179, 378
SfaNI GCATC 3 cut(s) 63, 1131, 1247
SfcI CTRYAG 2 cut(s) 449, 1188
SfiI GGCCNNNNNGGCC 1 cut(s) 1025
SfuI TTCGAA 1 cut(s) 164
SgrAI CRCCGGYG 1 cut(s) 1356
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
Sse9I AATT 9 cut(s) 32, 379, 523, 534, 540, 654, 918, 1173, 1314
SsiI CCGC 4 cut(s) 329, 435, 662, 947
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 2 cut(s) 179, 378
StyD4I CCNGG 2 cut(s) 694, 881
StyI CCWWGG 4 cut(s) 207, 604, 684, 834
TaaI ACNGT 3 cut(s) 95, 725, 771
TaiI ACGT 1 cut(s) 83
TaqI TCGA 3 cut(s) 36, 164, 430
TasI AATT 9 cut(s) 32, 379, 523, 534, 540, 654, 918, 1173, 1314
TauI GCSGC 3 cut(s) 332, 665, 950
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 5 cut(s) 186, 477, 582, 783, 962
Tru9I TTAA 5 cut(s) 186, 477, 582, 783, 962
TscAI CASTG 2 cut(s) 473, 1173
TseFI GTSAC 3 cut(s) 614, 1069, 1198
TseI GCWGC 5 cut(s) 326, 448, 451, 659, 1187
Tsp45I GTSAC 3 cut(s) 614, 1069, 1198
TspDTI ATGAA 7 cut(s) 149, 531, 808, 1053, 1149, 1169, 1271
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 2 cut(s) 473, 1173
Van91I CCANNNNNTGG 2 cut(s) 241, 701
XapI RAATTY 4 cut(s) 32, 523, 540, 1314
XceI RCATGY 2 cut(s) 45, 1077
XmiI GTMKAC 2 cut(s) 773, 1062
XmnI GAANNNNTTC 2 cut(s) 573, 1318
XspI CTAG 1 cut(s) 417
ZraI GACGTC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.