AT2G26740

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000795
Physical Location & Seq
Reverse (-)
11392849 .. 11394363
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G26740.1

Sequence Viewer

Length: 966 bp
ATGGAACACAGAAAGGTAAGAGGGAACGGCATAGACATTCACGTAGCAATCCAAGGTCCCTCCGATGGTCCTATAGTCCTCCTCCTCCATGGTTTCCCTGAGCTTTGGTACTCATGGCGCCACCAGATCCCTGGACTCGCCGCTCGTGGCTACCGTGCCGTCGCTCCTGACCTACGTGGTTACGGTGACTCCGATGCTCCCGCTGAGATCTCTTCCTACACATGCTTCAACATCGTTGGTGACTTAATCGCCGTCATATCCGCCTTAACCGCCTCAGAGGATGAGAAGGTCTTTGTGGTTGGACATGATTGGGGAGCTCTCATCGCTTGGTATTTATGTCTCTTCCGACCAGATAGAGTCAAAGCTCTGGTCAACCTCAGTGTTCCGTTCTCTTTCCGGCCAACCGATCCATCGGTGAAGCCCGTTGATCGCATGCGTGCTTTCTACGGCGATGATTACTATATCTGCAGGTTTCAGGAATTTGGAGATGTAGAAGCTGAGATTGCAGAGGTTGGAACAGAGAGAGTCATGAAGAGACTACTCACTTATAGAACTCCTGGACCGGTAATTATCCCGAAGGACAAAAGTTTTTGGGGATCCAAAGGCGAAACTATACCACTCCCGTCTTGGCTAACGGAGGAAGATGTTGCTTACTTCGTCAGCAAGTTTGAAGAGAAAGGCTTCTCTGGTCCAGTCAATTACTACCGTAACTTCAACAGGAACAATGAGTTGTTGGGTCCATGGGTGGGAAGCAAAATCCAGGTTCCTACAAAGTTTGTGATAGGGGAGCTTGATCTGGTCTACTATATGCCTGGTGTGAAGGAATATATACATGGTCCCCAGTTTAAGGAAGATGTGCCTTTGCTTGAAGAGCCTGTGGTCATGGAAGGAGTGGCTCACTTCATTAACCAAGAAAAGCCTCAGGAGATTCTCCAAATTATCCTTGATTTCATCTCCAAATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

321

Amino Acids

36.42

Weight (kDa)

5.28

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 24 - 137 1.6e-26 alpha/beta hydrolase fold
Hydrolase_4 PF12146 25 - 133 5.4e-13 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 26 - 311 1.4e-19 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 117
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 801
AciI CCGC 4 cut(s) 141, 201, 261, 270
AclWI GGATC 4 cut(s) 121, 401, 591, 604
AcoI YGGCCR 1 cut(s) 398
AcsI RAATTY 2 cut(s) 479, 959
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 1 cut(s) 110
AfiI CCNNNNNNNGG 3 cut(s) 65, 746, 847
AgeI ACCGGT 1 cut(s) 562
AgsI TTSAA 4 cut(s) 229, 671, 715, 869
AjnI CCWGG 4 cut(s) 130, 556, 759, 811
AluBI AGCT 5 cut(s) 103, 317, 365, 497, 790
AluI AGCT 5 cut(s) 103, 317, 365, 497, 790
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 2 cut(s) 344, 529
AlwI GGATC 4 cut(s) 121, 401, 591, 604
AoxI GGCC 1 cut(s) 398
ApoI RAATTY 2 cut(s) 479, 959
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
AsiGI ACCGGT 1 cut(s) 562
Asp700I GAANNNNTTC 1 cut(s) 680
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 6 cut(s) 56, 68, 560, 689, 737, 836
AsuHPI GGTGA 3 cut(s) 197, 251, 427
AvaII GGWCC 6 cut(s) 56, 68, 560, 689, 737, 836
AxyI CCTNAGG 1 cut(s) 921
BamHI GGATCC 1 cut(s) 596
BanI GGYRCC 1 cut(s) 117
BanII GRGCYC 1 cut(s) 319
BauI CACGAG 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 319
BccI CCATC 2 cut(s) 59, 418
BceAI ACGGC 4 cut(s) 43, 143, 236, 463
BciT130I CCWGG 4 cut(s) 132, 558, 761, 813
BcoDI GTCTC 2 cut(s) 344, 529
BfmI CTRYAG 2 cut(s) 72, 466
BfoI RGCGCY 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 459
BglII AGATCT 1 cut(s) 207
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 4 cut(s) 132, 558, 761, 813
Bme18I GGWCC 6 cut(s) 56, 68, 560, 689, 737, 836
BmgT120I GGNCC 6 cut(s) 56, 68, 560, 689, 737, 836
BmiI GGNNCC 6 cut(s) 58, 119, 598, 738, 765, 838
BmrFI CCNGG 4 cut(s) 132, 558, 761, 813
BmrI ACTGGG 1 cut(s) 835
BmsI GCATC 1 cut(s) 184
BmuI ACTGGG 1 cut(s) 835
Bpu10I CCTNAGC 1 cut(s) 99
BsaAI YACGTR 2 cut(s) 43, 176
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 4 cut(s) 52, 88, 130, 740
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 746, 847
Bse118I RCCGGY 1 cut(s) 562
Bse1I ACTGG 2 cut(s) 692, 841
Bse21I CCTNAGG 1 cut(s) 921
BseBI CCWGG 4 cut(s) 132, 558, 761, 813
BseDI CCNNGG 4 cut(s) 52, 88, 130, 740
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 3 cut(s) 65, 746, 847
BseMII CTCAG 6 cut(s) 90, 195, 288, 391, 489, 935
BseNI ACTGG 2 cut(s) 692, 841
BseRI GAGGAG 2 cut(s) 71, 74
BshFI GGCC 1 cut(s) 400
BshNI GGYRCC 1 cut(s) 117
BshTI ACCGGT 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 2 cut(s) 397, 563
BslFI GGGAC 2 cut(s) 42, 822
BslI CCNNNNNNNGG 3 cut(s) 65, 746, 847
BsmAI GTCTC 2 cut(s) 344, 529
BsmFI GGGAC 2 cut(s) 42, 822
BsnI GGCC 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 6 cut(s) 126, 207, 406, 427, 596, 793
Bsp19I CCATGG 2 cut(s) 88, 740
BspACI CCGC 4 cut(s) 141, 201, 261, 270
BspANI GGCC 1 cut(s) 400
BspCNI CTCAG 6 cut(s) 91, 196, 287, 390, 490, 934
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 6 cut(s) 58, 119, 598, 738, 765, 838
BspMAI CTGCAG 1 cut(s) 470
BspMI ACCTGC 1 cut(s) 459
BspPI GGATC 4 cut(s) 121, 401, 591, 604
BspQI GCTCTTC 1 cut(s) 864
BspT107I GGYRCC 1 cut(s) 117
BsrBI CCGCTC 1 cut(s) 143
BsrFI RCCGGY 1 cut(s) 562
BsrI ACTGG 2 cut(s) 692, 841
BssAI RCCGGY 1 cut(s) 562
BssECI CCNNGG 4 cut(s) 52, 88, 130, 740
BssMI GATC 6 cut(s) 126, 207, 406, 427, 596, 793
BssNI GRCGYC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 144
BssT1I CCWWGG 3 cut(s) 52, 88, 740
Bst2BI CACGAG 1 cut(s) 144
Bst2UI CCWGG 4 cut(s) 132, 558, 761, 813
Bst4CI ACNGT 3 cut(s) 155, 185, 707
Bst6I CTCTTC 5 cut(s) 217, 347, 527, 666, 864
BstACI GRCGYC 1 cut(s) 118
BstBAI YACGTR 2 cut(s) 43, 176
BstC8I GCNNGC 2 cut(s) 434, 438
BstDEI CTNAG 6 cut(s) 99, 204, 274, 377, 498, 921
BstDSI CCRYGG 2 cut(s) 88, 740
BstF5I GGATG 1 cut(s) 286
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 6 cut(s) 129, 210, 409, 430, 599, 796
BstMAI GTCTC 2 cut(s) 344, 529
BstMBI GATC 6 cut(s) 126, 207, 406, 427, 596, 793
BstMWI GCNNNNNNNGC 4 cut(s) 269, 323, 503, 871
BstNI CCWGG 4 cut(s) 132, 558, 761, 813
BstNSI RCATGY 2 cut(s) 225, 436
BstSCI CCNGG 4 cut(s) 130, 556, 759, 811
BstSFI CTRYAG 2 cut(s) 72, 466
BstX2I RGATCY 3 cut(s) 126, 207, 596
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 3 cut(s) 126, 207, 596
Bsu36I CCTNAGG 1 cut(s) 921
BsuRI GGCC 1 cut(s) 400
BtgI CCRYGG 2 cut(s) 88, 740
BtgZI GCGATG 2 cut(s) 307, 465
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 385
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 2 cut(s) 434, 438
CciI TCATGA 1 cut(s) 528
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 562
Cfr13I GGNCC 6 cut(s) 56, 68, 560, 689, 737, 836
Csp6I GTAC 1 cut(s) 109
CspAI ACCGGT 1 cut(s) 562
CviAII CATG 9 cut(s) 89, 114, 222, 305, 433, 529, 741, 833, 883
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 6 cut(s) 99, 204, 274, 377, 498, 921
DinI GGCGCC 1 cut(s) 119
DpnI GATC 6 cut(s) 128, 209, 408, 429, 598, 795
DpnII GATC 6 cut(s) 126, 207, 406, 427, 596, 793
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 5 cut(s) 217, 347, 527, 666, 864
EarI CTCTTC 5 cut(s) 217, 347, 527, 666, 864
EciI GGCGGA 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 317
Eco130I CCWWGG 3 cut(s) 52, 88, 740
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 6 cut(s) 56, 68, 560, 689, 737, 836
Eco53kI GAGCTC 1 cut(s) 317
Eco81I CCTNAGG 1 cut(s) 921
EcoICRI GAGCTC 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 4 cut(s) 130, 556, 759, 811
EcoT14I CCWWGG 3 cut(s) 52, 88, 740
EcoT38I GRGCYC 1 cut(s) 319
EgeI GGCGCC 1 cut(s) 119
EheI GGCGCC 1 cut(s) 119
ErhI CCWWGG 3 cut(s) 52, 88, 740
FaeI CATG 9 cut(s) 92, 117, 225, 308, 436, 532, 744, 836, 886
FaqI GGGAC 2 cut(s) 42, 822
FatI CATG 9 cut(s) 88, 113, 221, 304, 432, 528, 740, 832, 882
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 141
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 1 cut(s) 400
HapII CCGG 2 cut(s) 397, 563
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 9 cut(s) 92, 117, 225, 308, 436, 532, 744, 836, 886
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 5 cut(s) 135, 188, 357, 525, 928
HpaII CCGG 2 cut(s) 397, 563
HphI GGTGA 3 cut(s) 197, 251, 427
Hpy166II GTNNAC 2 cut(s) 373, 802
Hpy188I TCNGA 4 cut(s) 64, 193, 277, 347
Hpy188III TCNNGA 5 cut(s) 167, 476, 529, 574, 923
Hpy8I GTNNAC 2 cut(s) 373, 802
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 280, 571, 814, 881
HpyCH4III ACNGT 3 cut(s) 155, 185, 707
HpyCH4IV ACGT 2 cut(s) 42, 175
HpyCH4V TGCA 2 cut(s) 468, 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 323, 503, 871
HpyF3I CTNAG 6 cut(s) 99, 204, 274, 377, 498, 921
HpySE526I ACGT 2 cut(s) 42, 175
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 9 cut(s) 92, 117, 225, 308, 436, 532, 744, 836, 886
HspAI GCGC 1 cut(s) 118
KasI GGCGCC 1 cut(s) 117
Kzo9I GATC 6 cut(s) 126, 207, 406, 427, 596, 793
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 4 cut(s) 169, 202, 314, 787
LweI GCATC 1 cut(s) 184
MaeII ACGT 2 cut(s) 42, 175
MaeIII GTNAC 4 cut(s) 179, 185, 239, 707
MalI GATC 6 cut(s) 128, 209, 408, 429, 598, 795
MbiI CCGCTC 1 cut(s) 143
MboI GATC 6 cut(s) 126, 207, 406, 427, 596, 793
MboII GAAGA 7 cut(s) 204, 334, 544, 653, 683, 863, 881
MflI RGATCY 3 cut(s) 126, 207, 596
MhlI GDGCHC 1 cut(s) 319
MluCI AATT 5 cut(s) 479, 567, 697, 936, 959
Mly113I GGCGCC 1 cut(s) 118
MlyI GAGTC 4 cut(s) 129, 182, 366, 534
MmeI TCCRAC 3 cut(s) 280, 370, 493
MroXI GAANNNNTTC 1 cut(s) 680
MseI TTAA 4 cut(s) 245, 266, 846, 906
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 2 cut(s) 397, 563
MspR9I CCNGG 4 cut(s) 132, 558, 761, 813
MvaI CCWGG 4 cut(s) 132, 558, 761, 813
MwoI GCNNNNNNNGC 4 cut(s) 269, 323, 503, 871
NarI GGCGCC 1 cut(s) 118
NcoI CCATGG 2 cut(s) 88, 740
NdeII GATC 6 cut(s) 126, 207, 406, 427, 596, 793
NlaIII CATG 9 cut(s) 92, 117, 225, 308, 436, 532, 744, 836, 886
NlaIV GGNNCC 6 cut(s) 58, 119, 598, 738, 765, 838
NmuCI GTSAC 2 cut(s) 185, 239
NspI RCATGY 2 cut(s) 225, 436
PaeI GCATGC 1 cut(s) 436
PagI TCATGA 1 cut(s) 528
PciSI GCTCTTC 1 cut(s) 864
PcsI WCGNNNNNNNCGW 2 cut(s) 151, 189
PdmI GAANNNNTTC 1 cut(s) 680
PfeI GAWTC 1 cut(s) 928
PfoI TCCNGGA 1 cut(s) 556
PinAI ACCGGT 1 cut(s) 562
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 4 cut(s) 129, 182, 365, 533
PluTI GGCGCC 1 cut(s) 121
PpsI GAGTC 4 cut(s) 129, 182, 365, 533
Ppu21I YACGTR 2 cut(s) 43, 176
PpuMI RGGWCCY 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 4 cut(s) 130, 556, 759, 811
PspGI CCWGG 4 cut(s) 130, 556, 759, 811
PspN4I GGNNCC 6 cut(s) 58, 119, 598, 738, 765, 838
PspPI GGNCC 6 cut(s) 56, 68, 560, 689, 737, 836
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 470
PsuI RGATCY 3 cut(s) 126, 207, 596
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SacI GAGCTC 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 864
SaqAI TTAA 4 cut(s) 245, 266, 846, 906
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 6 cut(s) 126, 207, 406, 427, 596, 793
Sau96I GGNCC 6 cut(s) 56, 68, 560, 689, 737, 836
SchI GAGTC 4 cut(s) 129, 182, 366, 534
ScrFI CCNGG 4 cut(s) 132, 558, 761, 813
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 2 cut(s) 72, 466
SfoI GGCGCC 1 cut(s) 119
SinI GGWCC 6 cut(s) 56, 68, 560, 689, 737, 836
SphI GCATGC 1 cut(s) 436
Sse9I AATT 5 cut(s) 479, 567, 697, 936, 959
SsiI CCGC 4 cut(s) 141, 201, 261, 270
SspDI GGCGCC 1 cut(s) 117
SstI GAGCTC 1 cut(s) 319
StyD4I CCNGG 4 cut(s) 130, 556, 759, 811
StyI CCWWGG 3 cut(s) 52, 88, 740
TaaI ACNGT 3 cut(s) 155, 185, 707
TaiI ACGT 2 cut(s) 45, 178
TasI AATT 5 cut(s) 479, 567, 697, 936, 959
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 928
Tru1I TTAA 4 cut(s) 245, 266, 846, 906
Tru9I TTAA 4 cut(s) 245, 266, 846, 906
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 2 cut(s) 185, 239
Tsp45I GTSAC 2 cut(s) 185, 239
TspDTI ATGAA 3 cut(s) 545, 892, 940
TspGWI ACGGA 2 cut(s) 375, 650
TspRI CASTG 1 cut(s) 385
VpaK11BI GGWCC 6 cut(s) 56, 68, 560, 689, 737, 836
XapI RAATTY 2 cut(s) 479, 959
XceI RCATGY 2 cut(s) 225, 436
XcmI CCANNNNNNNNNTGG 2 cut(s) 128, 624
XmiI GTMKAC 1 cut(s) 801
XmnI GAANNNNTTC 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.