AT2G26750

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000795
Physical Location & Seq
Reverse (-)
11395707 .. 11397219
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G26750.1

Sequence Viewer

Length: 963 bp
ATGGAGCACAGAAATGTAAGAGGGAACGGCATAGACATCCACGTAGCAATCCAAGGTCCCTCCGATGGTACTATAGTCCTCCTCCTCCATGGTTTCCCTGAGCTTTGGTACTCATGGCGCCACCAGATCTCCGGACTCGCCGCTCGTGGCTACCGTGCCGTCGCTCCTGACCTACGTGGTTACGGTGACTCCGATGCTCCGGCTGAGATTTCTTCCTTTACATGCTTTAACATAGTAGGTGACTTAGTCGCCGTCATATCCACCTTAATCAAGGAGGATAAGAAGGTGTTTGTGGTTGGACATGATTGGGGAGCTCTCATCGCTTGGTACCTTTGTCTTTTTAGACCAGATAAAGTCAAAGCTTTGGTTAACCTTAGTGTTCCGTTGTCTTTCTGGCCAACCGATCCATCGGTGAAGCCCGTTGATCGCATGCGTGCTGTATACGGCAATGATTACTATGTCTGCAGGTTTCAGGAAGTTGGAGATATAGAAGCTGAGATTGCAGAGGTTGGAACAGAGAGAGTCATGAAGAGACTACTCACTTATAGAACTCCTGGACCGCTTATTATACCGAAGGACAAAAGTTTTTGGGGATCCAAAGGCGAAACTATACCACTACCGTCTTGGCTAACGGAGGAAGATGTTGCTTACTTCGTCAGCAAGTTTAAAGAGAAAGGCTTCTGTGGTCCAGTCAATTACTACCGTAACTTCAACAGGAACAATGAGTTGTTGGGTCCATGGGTGGGAAGCAAAATCCAGGTTCCTACAAAGTTTGTGATAGGGGAGCTTGATCTGGTCTACTATATGCCTGGTGTGAAGGAATATATACATGGTCCCCAGTTTAAGGAAGATGTGCCTTTGATTGAAGAGCCTGTGGTCATGGAAGGAGTGGCTCACTTTCTTAACCAAGAGAAGCCTCAAGAGATTCTCCAAATAATCCTTGATTTCATCTCCACATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

320

Amino Acids

36.26

Weight (kDa)

5.58

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 25 - 133 5.9e-25 alpha/beta hydrolase fold
Hydrolase_4 PF12146 25 - 127 1.8e-13 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 26 - 310 2e-19 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
Acc65I GGTACC 1 cut(s) 327
AccB1I GGYRCC 2 cut(s) 117, 327
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 441, 798
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 2 cut(s) 141, 560
AclWI GGATC 3 cut(s) 398, 588, 601
AcoI YGGCCR 1 cut(s) 395
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 3 cut(s) 70, 110, 329
AfiI CCNNNNNNNGG 3 cut(s) 65, 743, 844
AgsI TTSAA 2 cut(s) 712, 866
AjnI CCWGG 3 cut(s) 553, 756, 808
AluBI AGCT 5 cut(s) 103, 314, 362, 494, 787
AluI AGCT 5 cut(s) 103, 314, 362, 494, 787
Alw21I GWGCWC 2 cut(s) 9, 316
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 3 cut(s) 398, 588, 601
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 1 cut(s) 395
Asp700I GAANNNNTTC 1 cut(s) 677
Asp718I GGTACC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 5 cut(s) 56, 557, 686, 734, 833
AsuHPI GGTGA 3 cut(s) 197, 251, 424
AvaII GGWCC 5 cut(s) 56, 557, 686, 734, 833
BaeI ACNNNNGTAYC 4 cut(s) 60, 93, 319, 352
BalI TGGCCA 1 cut(s) 397
BamHI GGATCC 1 cut(s) 593
BanI GGYRCC 2 cut(s) 117, 327
BanII GRGCYC 1 cut(s) 316
BauI CACGAG 1 cut(s) 144
Bbv12I GWGCWC 2 cut(s) 9, 316
BccI CCATC 2 cut(s) 59, 415
BceAI ACGGC 4 cut(s) 43, 143, 236, 460
BciT130I CCWGG 3 cut(s) 555, 758, 810
BcoDI GTCTC 1 cut(s) 526
BfmI CTRYAG 2 cut(s) 72, 463
BfoI RGCGCY 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 456
BglII AGATCT 1 cut(s) 126
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 3 cut(s) 555, 758, 810
Bme18I GGWCC 5 cut(s) 56, 557, 686, 734, 833
BmgT120I GGNCC 5 cut(s) 56, 557, 686, 734, 833
BmiI GGNNCC 7 cut(s) 58, 119, 329, 595, 735, 762, 835
BmrFI CCNGG 3 cut(s) 555, 758, 810
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 1 cut(s) 184
BmuI ACTGGG 1 cut(s) 832
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 903
BsaAI YACGTR 2 cut(s) 43, 176
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 3 cut(s) 52, 88, 737
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 173, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 743, 844
Bse1I ACTGG 2 cut(s) 689, 838
Bse3DI GCAATG 1 cut(s) 454
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 3 cut(s) 555, 758, 810
BseDI CCNNGG 3 cut(s) 52, 88, 737
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 3 cut(s) 65, 743, 844
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 3 cut(s) 90, 195, 486
BseNI ACTGG 2 cut(s) 689, 838
BseRI GAGGAG 2 cut(s) 71, 74
BshFI GGCC 1 cut(s) 397
BshNI GGYRCC 2 cut(s) 117, 327
BsiHKAI GWGCWC 2 cut(s) 9, 316
BsiSI CCGG 2 cut(s) 132, 200
BslFI GGGAC 2 cut(s) 42, 819
BslI CCNNNNNNNGG 3 cut(s) 65, 743, 844
BsmAI GTCTC 1 cut(s) 526
BsmFI GGGAC 2 cut(s) 42, 819
BsnI GGCC 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 9, 316
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 5 cut(s) 126, 403, 424, 593, 790
Bsp19I CCATGG 2 cut(s) 88, 737
BspACI CCGC 2 cut(s) 141, 560
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 3 cut(s) 91, 196, 487
BspEI TCCGGA 1 cut(s) 131
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 7 cut(s) 58, 119, 329, 595, 735, 762, 835
BspMAI CTGCAG 1 cut(s) 467
BspMI ACCTGC 1 cut(s) 456
BspPI GGATC 3 cut(s) 398, 588, 601
BspQI GCTCTTC 1 cut(s) 861
BspT107I GGYRCC 2 cut(s) 117, 327
BsrBI CCGCTC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 454
BsrI ACTGG 2 cut(s) 689, 838
BssECI CCNNGG 3 cut(s) 52, 88, 737
BssMI GATC 5 cut(s) 126, 403, 424, 593, 790
BssNAI GTATAC 1 cut(s) 442
BssNI GRCGYC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 144
BssT1I CCWWGG 3 cut(s) 52, 88, 737
Bst1107I GTATAC 1 cut(s) 442
Bst2BI CACGAG 1 cut(s) 144
Bst2UI CCWGG 3 cut(s) 555, 758, 810
Bst4CI ACNGT 4 cut(s) 155, 185, 621, 704
Bst6I CTCTTC 2 cut(s) 524, 861
BstACI GRCGYC 1 cut(s) 118
BstBAI YACGTR 2 cut(s) 43, 176
BstC8I GCNNGC 2 cut(s) 431, 435
BstDEI CTNAG 5 cut(s) 99, 204, 244, 374, 495
BstDSI CCRYGG 2 cut(s) 88, 737
BstF5I GGATG 1 cut(s) 36
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 5 cut(s) 129, 406, 427, 596, 793
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 5 cut(s) 126, 403, 424, 593, 790
BstMWI GCNNNNNNNGC 2 cut(s) 320, 500
BstNI CCWGG 3 cut(s) 555, 758, 810
BstNSI RCATGY 2 cut(s) 225, 433
BstSCI CCNGG 3 cut(s) 553, 756, 808
BstSFI CTRYAG 2 cut(s) 72, 463
BstX2I RGATCY 2 cut(s) 126, 593
BstYI RGATCY 2 cut(s) 126, 593
BstZ17I GTATAC 1 cut(s) 442
BsuRI GGCC 1 cut(s) 397
BtgI CCRYGG 2 cut(s) 88, 737
BtgZI GCGATG 1 cut(s) 304
BtsCI GGATG 1 cut(s) 36
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 431, 435
CciI TCATGA 1 cut(s) 525
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 5 cut(s) 56, 557, 686, 734, 833
Csp6I GTAC 3 cut(s) 69, 109, 328
CviAII CATG 9 cut(s) 89, 114, 222, 302, 430, 526, 738, 830, 880
CviQI GTAC 3 cut(s) 69, 109, 328
DdeI CTNAG 5 cut(s) 99, 204, 244, 374, 495
DinI GGCGCC 1 cut(s) 119
DpnI GATC 5 cut(s) 128, 405, 426, 595, 792
DpnII GATC 5 cut(s) 126, 403, 424, 593, 790
DraI TTTAAA 1 cut(s) 667
EaeI YGGCCR 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 524, 861
EarI CTCTTC 2 cut(s) 524, 861
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 3 cut(s) 52, 88, 737
Eco24I GRGCYC 1 cut(s) 316
Eco47I GGWCC 5 cut(s) 56, 557, 686, 734, 833
Eco53kI GAGCTC 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 56
EcoRII CCWGG 3 cut(s) 553, 756, 808
EcoT14I CCWWGG 3 cut(s) 52, 88, 737
EcoT38I GRGCYC 1 cut(s) 316
EgeI GGCGCC 1 cut(s) 119
EheI GGCGCC 1 cut(s) 119
ErhI CCWWGG 3 cut(s) 52, 88, 737
FaeI CATG 9 cut(s) 92, 117, 225, 305, 433, 529, 741, 833, 883
FaqI GGGAC 2 cut(s) 42, 819
FatI CATG 9 cut(s) 88, 113, 221, 301, 429, 525, 737, 829, 879
FblI GTMKAC 2 cut(s) 441, 798
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 23
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 1 cut(s) 141
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 1 cut(s) 397
HapII CCGG 2 cut(s) 132, 200
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 9 cut(s) 92, 117, 225, 305, 433, 529, 741, 833, 883
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 4 cut(s) 135, 188, 522, 925
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 2 cut(s) 132, 200
HphI GGTGA 3 cut(s) 197, 251, 424
Hpy166II GTNNAC 3 cut(s) 370, 442, 799
Hpy188I TCNGA 2 cut(s) 64, 193
Hpy188III TCNNGA 5 cut(s) 132, 167, 473, 526, 920
Hpy8I GTNNAC 3 cut(s) 370, 442, 799
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 277, 568, 811, 878
HpyCH4III ACNGT 4 cut(s) 155, 185, 621, 704
HpyCH4IV ACGT 2 cut(s) 42, 175
HpyCH4V TGCA 2 cut(s) 465, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 500
HpyF3I CTNAG 5 cut(s) 99, 204, 244, 374, 495
HpySE526I ACGT 2 cut(s) 42, 175
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 9 cut(s) 92, 117, 225, 305, 433, 529, 741, 833, 883
HspAI GCGC 1 cut(s) 118
KasI GGCGCC 1 cut(s) 117
Kpn2I TCCGGA 1 cut(s) 131
KpnI GGTACC 1 cut(s) 331
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 5 cut(s) 126, 403, 424, 593, 790
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 5 cut(s) 4, 169, 202, 311, 784
LweI GCATC 1 cut(s) 184
MaeII ACGT 2 cut(s) 42, 175
MaeIII GTNAC 4 cut(s) 179, 185, 239, 704
MalI GATC 5 cut(s) 128, 405, 426, 595, 792
MbiI CCGCTC 1 cut(s) 143
MboI GATC 5 cut(s) 126, 403, 424, 593, 790
MboII GAAGA 5 cut(s) 204, 541, 650, 860, 878
MflI RGATCY 2 cut(s) 126, 593
MhlI GDGCHC 2 cut(s) 9, 316
MlsI TGGCCA 1 cut(s) 397
MluCI AATT 1 cut(s) 694
MluNI TGGCCA 1 cut(s) 397
Mly113I GGCGCC 1 cut(s) 118
MlyI GAGTC 3 cut(s) 129, 182, 531
MmeI TCCRAC 3 cut(s) 277, 460, 490
MnlI CCTC 9 cut(s) 14, 70, 89, 92, 95, 268, 499, 628, 927
Mox20I TGGCCA 1 cut(s) 397
MroI TCCGGA 1 cut(s) 131
MroXI GAANNNNTTC 1 cut(s) 677
MscI TGGCCA 1 cut(s) 397
MseI TTAA 6 cut(s) 228, 266, 369, 666, 843, 903
MslI CAYNNNNRTG 1 cut(s) 12
Msp20I TGGCCA 1 cut(s) 397
MspI CCGG 2 cut(s) 132, 200
MspR9I CCNGG 3 cut(s) 555, 758, 810
MvaI CCWGG 3 cut(s) 555, 758, 810
MwoI GCNNNNNNNGC 2 cut(s) 320, 500
NarI GGCGCC 1 cut(s) 118
NcoI CCATGG 2 cut(s) 88, 737
NdeII GATC 5 cut(s) 126, 403, 424, 593, 790
NlaIII CATG 9 cut(s) 92, 117, 225, 305, 433, 529, 741, 833, 883
NlaIV GGNNCC 7 cut(s) 58, 119, 329, 595, 735, 762, 835
NmuCI GTSAC 2 cut(s) 185, 239
NspI RCATGY 2 cut(s) 225, 433
PaeI GCATGC 1 cut(s) 433
PagI TCATGA 1 cut(s) 525
PciSI GCTCTTC 1 cut(s) 861
PcsI WCGNNNNNNNCGW 2 cut(s) 151, 189
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 1 cut(s) 925
PflFI GACNNNGTC 1 cut(s) 245
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 3 cut(s) 129, 182, 530
PluTI GGCGCC 1 cut(s) 121
PpsI GAGTC 3 cut(s) 129, 182, 530
Ppu21I YACGTR 2 cut(s) 43, 176
PpuMI RGGWCCY 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 56
Psp6I CCWGG 3 cut(s) 553, 756, 808
PspGI CCWGG 3 cut(s) 553, 756, 808
PspN4I GGNNCC 7 cut(s) 58, 119, 329, 595, 735, 762, 835
PspPI GGNCC 5 cut(s) 56, 557, 686, 734, 833
PspPPI RGGWCCY 1 cut(s) 56
PstI CTGCAG 1 cut(s) 467
PsuI RGATCY 2 cut(s) 126, 593
PsyI GACNNNGTC 1 cut(s) 245
RsaI GTAC 3 cut(s) 70, 110, 329
RsaNI GTAC 3 cut(s) 69, 109, 328
RseI CAYNNNNRTG 1 cut(s) 12
SacI GAGCTC 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 6 cut(s) 228, 266, 369, 666, 843, 903
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 5 cut(s) 126, 403, 424, 593, 790
Sau96I GGNCC 5 cut(s) 56, 557, 686, 734, 833
SchI GAGTC 3 cut(s) 129, 182, 531
ScrFI CCNGG 3 cut(s) 555, 758, 810
SduI GDGCHC 2 cut(s) 9, 316
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 2 cut(s) 72, 463
SfoI GGCGCC 1 cut(s) 119
SinI GGWCC 5 cut(s) 56, 557, 686, 734, 833
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 918
SmoI CTYRAG 1 cut(s) 918
SphI GCATGC 1 cut(s) 433
Sse9I AATT 1 cut(s) 694
SsiI CCGC 2 cut(s) 141, 560
SspDI GGCGCC 1 cut(s) 117
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 553, 756, 808
StyI CCWWGG 3 cut(s) 52, 88, 737
TaaI ACNGT 4 cut(s) 155, 185, 621, 704
TaiI ACGT 2 cut(s) 45, 178
TasI AATT 1 cut(s) 694
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 925
Tru1I TTAA 6 cut(s) 228, 266, 369, 666, 843, 903
Tru9I TTAA 6 cut(s) 228, 266, 369, 666, 843, 903
TseFI GTSAC 2 cut(s) 185, 239
Tsp45I GTSAC 2 cut(s) 185, 239
TspDTI ATGAA 2 cut(s) 542, 937
TspGWI ACGGA 2 cut(s) 372, 647
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 5 cut(s) 56, 557, 686, 734, 833
XceI RCATGY 2 cut(s) 225, 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmiI GTMKAC 2 cut(s) 441, 798
XmnI GAANNNNTTC 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.