Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002740

Physical Location & Seq

Genomic Coordinates Reverse (-)

2586804 .. 2595654

Gene Structure 8851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G08530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5112 bp

ATGGCGGCTGCCAACGCCCCCATCACGATGAAGGAGGTCCTGACGCTTCCGAGCATCGGGATCAATCAGCAATTCATCACGTTTACAAATGTTACTATGGAGTCTGACAAGTATATCTGTGTACGAGAGACGTCACCGCAAAACAGTGTTGTAATTATAGATATGAATATGCCGATGCAGCCTCTTAGGAGGCCTATTACTGCAGACTCTGCTCTTATGAACCCAAACTCGAAGATCCTTGCCTTGAAAGCTCAAGTTCCAGGAACTACTCAGGATCATCTACAGATATTTAACATTGAAGCCAAAGCAAAGTTGAAATCACATCAGATGCCTGAGCAGGTTGTCTTTTGGAAGTGGATTACACCAAAGATGTTGGGGCTGGTCACACAGAATTCCGTCTATCATTGGTCAATTGAAGGTGATTCTGAGCCAGTTAAGATGTTTGATAGAACAGCCAATTTGGCCAACAATCAAATTATAAACTATAAGTGCTCTCCTAATGAGAAGTGGTTGGTTTTAATTGGAATTGCTCCTGGCTCTCCTGAGAGACAACAATTGGTAAAAGGAAATATGCAGCTTTTCTCTGTGGATCAACAGCGGAGTCAGGCCCTTGAGGCACATGCTGCATCGTTTGCGCAGTTTAAGGTTCCTGGGAATGAGAATCCTTCTATTCTTATATCATTTGCAAGCAAGAGCTTTAATGCTGGGCAGATAACATCAAAGTTGCATGTCATTGAGCTTGGTGCCCAACCTGGAAAACCATCATTTACAAAAAAACAAGCAGATCTCTTTTTTCCCCCTGATTTTGCTGATGATTTTCCTGTTGCCATGCAGGTCTCTCACAAATTTAACTTGATATATGTTATCACCAAACTTGGCCTGTTGTTTGTGTACGATCTAGAGACTGCTTCTGCTATCTACAGAAATCGGATTAGTCCAGACCCAATTTTCTTGACTTCTGAAGCTTCCTCAGTTGGGGGTTTCTATGCCATTAATAGGCGAGGACAAGTTCTTTTGGCTACAGTAAACGAGGCTACCATTATACCTTTTATTAGTGGCCAATTGAACAATTTGGAGCTTGCTGTCAATCTTGCTAAAAGAGGAAACCTCCCAGGTGCAGAAAATCTGGTTGTCCAGCGGTTCCAGGAGTTGTTCGCTCAAACAAAGTACAAGGAGGCTGCTGAGCTTGCTGCTGAATCTCCTCAAGGAATTCTAAGGACACCTGATACAGTGGCCAAATTCCAGAGTGTTCCTGTACAAGCTGGACAAACTCCTCCATTGTTACAGTATTTTGGGACTCTTTTGACTAGAGGGAAACTCAATTCATATGAGTCACTGGAACTATCTCGGCTGGTTGTGAACCAAAACAAGAAGAACCTCCTGGAAAACTGGTTGGCAGAGGATAAACTAGAATGCAGTGAAGAACTTGGAGACCTTGTGAAGACTGTGGATAATGATCTCGCTCTGAAAATATACATCAAAGCTCGGGCGACTCCAAAAGTTGTAGCAGCTTTTGCAGAAAGAAGGGAGTTTGACAAAATACTGATTTACTCAAAGCAGGTTGGGTACACACCTGATTACCTGTTTCTTCTGCAAACCATACTCCGTACAGATCCTCAGGGAGCAGTAAATTTTGCATTAATGATGTCTCAAATGGAAGGAGGTTCTCCAGTTGACTACAACACCATCACTGATCTTTTTCTTCAGAGAAATCTGATCCGTGAAGCGACTTCTTTCCTTCTGGATGTGCTAAAGCCAAATTTACCTGAGCATGCTTTTCTGCAAACTAAGGTTCTGGAGATAAATTTGGTAACTTTTCCTAATGTGGCTGATGCGGTATTAGCAAATGGGATGTTCACCCACTATGACCGGCCTCGCATTGCTCAACTTTGTGAAAAGGCTGGACTTTATATCCAATCCTTGAAGCATTACTCAGAGTTACCTGATATAAAACGTGTAATTGTAAACACACATGCTATCGAGCCACAGGCTCTTGTTGAGTTTTTTGGCACTCTTTCTAGCGAGTGGGCTATGGAGTGCATGAAGGATCTGTTGCTGGTCAACCTGAGAGGCAATCTGCAGATAATTGTTCAGGCTTGCAAGGAGTACTGTGAGCAACTTGGTGTTGATGCATGTATTAAGCTCTTTGAGCAGTTCAAGTCATATGAAGGGCTATACTTTTTCCTAGGTTCATATTTGAGTATGAGTGAGGATCCTGAGATCCATTTCAAGTACATTGAAGCAGCTGCCAAGACTGGTCAAATAAAGGAGGTTGAGCGTGTAACTAGAGAGTCTAACTTCTATGATGCTGAAAAGACGAAGAACTTTTTGATGGAAGCTAAGCTGCCTGATGCACGACCTTTGATAAATGTCTGTGATCGTTTCAGCTTTGTGCCTGATCTTACTCATTACCTCTACACAAACAACATGCTTCGTTACATTGAAGGCTACGTGCAGAAGGTGAACCCTGGGAATGCTCCCTTAGTTGTCGGGCAGTTGCTTGATGATGAATGCCCTGAAGATTTTATAAAAGGCCTCATTCTCTCTGTTCGTTCATTGCTTCCTGTTGAACCACTTGTCGAGGAATGTGAGAAGAGAAATCGACTCCGTCTCCTCACTCAGTTCTTGGAGCATTTAGTTAGTGAGGGAAGCCAAGATGTGCATGTCCATAATGCCTTGGGTAAAATTATCATAGACAGTAACAACAACCCTGAGCATTTCCTGACCACCAACCCTTACTACGACTCTAAGGTTGTGGGTAAGTACTGCGAGAAACGTGATCCTACCCTAGCAGTTGTGGCATACAGAAGAGGACAATGTGACGAGGAACTCATCAATGTCACTAACAAAAACTCTTTGTTTAAGTTACAAGCCAGGTATGTAGTGGAGAGGATGGATGGTGATCTGTGGGATAAGGTTCTCGATGAAAACAATGATTATAGAAGACAGCTTATTGACCAAGTTGTGTCTACTGCTTTACCTGAAAGCAAGAGCCCAGAGCAAGTTTCTGCAGCTGTTAAAGCATTCATGACTGCTGATTTGCCGCACGAGCTTATTGAGCTTCTTGAAAAGATTGTTCTACAAAATTCTGCCTTCAGTGGAAACTTCAATCTGCAAAATCTGCTTATATTGACAGCTATTAAGGCAGATCCGTCTAGAGTTATGGATTACATCAACAGGCTGGACAACTTTGATGGACCTGCAGTTGGAGAAGTCGCAGTTGAAGCCCAATTATATGAGGAAGCATTTGCTATCTTCAAGAAGTTCAACTTAAATGTTCAGGCTGTCAATGTATTGCTGGATAATGTCAGGAGCATTGAGCGTGCAGTTGAGTTTGCATTCCGGGTCGAAGAAGATTCTGTTTGGAGCCAAGTCGCTAAGGCGCAACTCAGGGAAGGACTAGTCAGTGATGCAATTGAGTCCTTTATCCGTGCTGATGATGCCACTCATTTCTTGGAGGTCATACGTGTCTCTGAAGATACTGATGTCTATGATGACTTGGTCAAATACCTTCTGATGGTTAGACAGAAGGTGAAGGAACCCAAGGTTGATAGTGAGCTCATCTATGCATATGCAAAGATTGATCGGTTAGGTGAGATTGAAGAATTCATTCTGATGCCAAACGTTGCTAATCTACAACATGTTGGTGATCGTCTGTATGACGAAGCTCTCTATGAGGCTGCAAAAATAATCTATGCGTTCATATCAAACTGGGGCAAGTTAGCCGTCACACTTGTGAAGTTGCAACAGTTCCAAGGTGCTGTTGATGCTGCAAGGAAAGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGGTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTCAATGAATTAATCTCACTTATGGAGAGTGGACTTGGGCTTGAACGTGCTCACATGGGTATCTTCACCGAGTTGGGTGTACTCTATGCTAGATATCGTTATGAGAAACTTATGGAACACATCAAGTTATTTTCAACTCGCCTCAATATTCCCAAGCTTATACGGGCCTGTGATGAACAACAGCACTGGCAGGAACTTACCTATCTGTACATTCAATATGATGAGTTTGATAATGCTGCGACCACCGTCATGAACCATTCTCCTGAAGCATGGGAGCACATGCAATTCAAAGACATTGTTGCCAAGGTAGCGAATGTTGAACTGTACTACAAGGCTGTGCACTTCTACTTGCAAGAGCATCCGGATATAATCAACGATCTTCTCAACGTACTTGCGTTGCGGTTAGACCATACTCGTGTTGTTGATATAATGCGGAAGGCTGGTCACTTGCGTCTTATCAAGCCATACATGATTGCAGTTCAGAGCAACAATGTCTCTGCTGTTAATGAGGCTCTGAATGAGATATACGTGGAAGAAGAAGACTACGATAGGTTGCGAGAGTCTATTGATTTACATGACAGCTTTGACCAGATTGGCCTCGCTCAGAAGATCGAGAAACACGAACTTGTTGAAATGAGACGTGTGGCTGCTTATATCTACAAGAAAGCGGGCAGATGGAAGCAATCAATTGCATTATCAAAGAAAGACAACATGTACAAGGACTGTATGGAAACGGCTTCACAATCAGGCGAACATGAACTTGCAGAACAACTACTGGTGTATTTCATTGAACAGGGCAAAAAGGAATGCTTTGCTACATGTCTCTTTGTATGTTATGACTTAATCCGACCAGACGTGGCTCTAGAACTTGCCTGGATCAACAATATGATGGACTTTGCCTTCCCGTATCTCCTCCAGTTTATCCGAGAGTACTCTGGCAAAGTGGATGAGCTAATAAAGGACAAGCTTGAGGCACAAAAAGAGGTGAAGGCCAAAGAGCAGGAAGAGAAGGATGTCATATCCCAACAGAACATGTATGCCCAAATGTTGCCACTTGCTTTACCTGCACCACCGATGCCAGGAATGGGAGGTGGTGGTGGGTATGGTCCTCCACCACAAATGGGCGGTATGCCAGGAATGCCTCCCATGCCACCATATGGAATGCCACCGATGGGCGGCTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006897 GO:0006898 GO:0008150 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010118 GO:0012505 GO:0016020 GO:0016192 GO:0030054 GO:0031090 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0071944 GO:0072583 GO:0098588 GO:0098657 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100

Pfam Domains

Protein Families

Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1703

Amino Acids

193.27

Weight (kDa)

5.25

Isoelectric Point (pI)

42.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clathrin_propel	PF01394	20 - 56	8.4e-08	Clathrin propeller repeat
Clathrin-link	PF09268	344 - 367	3.4e-09	Clathrin, heavy-chain linker
Clathrin_H_link	PF13838	369 - 434	4e-28	Clathrin-H-link
Clathrin	PF00637	553 - 692	9.4e-26	Region in Clathrin and VPS
Clathrin	PF00637	701 - 841	3.8e-27	Region in Clathrin and VPS
Clathrin	PF00637	850 - 984	7.4e-34	Region in Clathrin and VPS
Clathrin	PF00637	993 - 1131	2e-31	Region in Clathrin and VPS
Clathrin	PF00637	1144 - 1281	1.1e-30	Region in Clathrin and VPS
Clathrin	PF00637	1290 - 1431	2e-26	Region in Clathrin and VPS
Clathrin	PF00637	1438 - 1579	2.9e-32	Region in Clathrin and VPS

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002740)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08530 AT3G11130
fragaria_vesca	FvH4_3g39020 FvH4_7g24660 FvH4_7g24660
malus_domestica	MD01G1154700.v1.1 MD03G1065300.v1.1 MD07G1223800.v1.1 MD11G1070100.v1.1
prunus_persica	Prupe.2G252300_v2.0.a1 Prupe.6G054300_v2.0.a1 Prupe.6G054300_v2.0.a1 Prupe.6G054300_v2.0.a1 Prupe.6G054300_v2.0.a1
pyrus_communis	pycom01g17210 pycom03g05230 pycom07g20230 pycom07g20240 pycom11g05860
rosa_chinensis	RchiOBHm_Chr1g0370491 RchiOBHm_Chr5g0070331
rosa_laevigata	RLG00000027035 RLG00000036125
rosa_multiflora	Rmu_sc0002558.1_g000009 Rmu_sc0007961.1_g000022
rosa_roxburghii	Rroxscaffold_1G00010850 Rroxscaffold_4G00286510
rosa_rugosa	Rorug01G0357300 Rorug05G0404900
rosa_samantha	Rh1AG365800 Rh1BG329500 Rh1CG341800 Rh1CG343600 Rh5AG460600 Rh5BG478900 Rh5CG503100 Rh5DG490800
rosa_wichuraiana	Rw1G032230 Rw5G042930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	479, 2528
AatII	GACGTC	1 cut(s)	134
Acc16I	TGCGCA	1 cut(s)	636
Acc36I	ACCTGC	5 cut(s)	328, 823, 1549, 3208, 4999
AccB1I	GGYRCC	1 cut(s)	743
AccB7I	CCANNNNNTGG	1 cut(s)	5084
AccI	GTMKAC	1 cut(s)	2969
AccIII	TCCGGA	1 cut(s)	4288
AclI	AACGTT	1 cut(s)	3624
AcoI	YGGCCR	3 cut(s)	462, 1057, 1233
AcuI	CTGAAG	6 cut(s)	981, 1688, 2538, 3079, 3495, 4212
AcyI	GRCGYC	1 cut(s)	131
AflIII	ACRYGT	7 cut(s)	1954, 3466, 3640, 4567, 4638, 4745, 4959
AhlI	ACTAGT	1 cut(s)	3400
AjiI	CACGTC	2 cut(s)	4568, 4783
AleI	CACNNNNGTG	1 cut(s)	3734
Alw21I	GWGCWC	5 cut(s)	494, 3561, 3979, 4206, 4269
Alw44I	GTGCAC	1 cut(s)	4265
AlwNI	CAGNNNCTG	1 cut(s)	209
Ama87I	CYCGRG	1 cut(s)	1485
Aor13HI	TCCGGA	1 cut(s)	4288
ApaLI	GTGCAC	1 cut(s)	4265
ArsI	GACNNNNNNTTYG	4 cut(s)	3022, 3054, 3844, 3876
AseI	ATTAAT	3 cut(s)	993, 1640, 3938
Asp700I	GAANNNNTTC	1 cut(s)	3609
AspA2I	CCTAGG	1 cut(s)	2185
AspLEI	GCGC	2 cut(s)	637, 3385
AspS9I	GGNCC	5 cut(s)	37, 607, 3197, 4092, 5033
AsuC2I	CCSGG	1 cut(s)	3344
AvaI	CYCGRG	1 cut(s)	1485
AvaII	GGWCC	3 cut(s)	37, 3197, 5033
AvrII	CCTAGG	1 cut(s)	2185
AxyI	CCTNAGG	1 cut(s)	1617
BaeGI	GKGCMC	2 cut(s)	748, 4269
BaeI	ACNNNNGTAYC	4 cut(s)	3471, 3504, 3970, 4003
BalI	TGGCCA	3 cut(s)	464, 1059, 1235
BamHI	GGATCC	1 cut(s)	2212
BanI	GGYRCC	1 cut(s)	743
BanII	GRGCYC	2 cut(s)	2996, 3561
BauI	CACGAG	2 cut(s)	3047, 4341
BbsI	GAAGAC	3 cut(s)	1448, 2950, 4473
Bbv12I	GWGCWC	5 cut(s)	494, 3561, 3979, 4206, 4269
BbvCI	CCTCAGC	1 cut(s)	3831
BceAI	ACGGC	2 cut(s)	3710, 4677
BcnI	CCSGG	1 cut(s)	3344
BcuI	ACTAGT	1 cut(s)	3400
BfmI	CTRYAG	7 cut(s)	201, 281, 919, 1020, 2078, 3009, 3201
BfuAI	ACCTGC	5 cut(s)	328, 823, 1549, 3208, 4999
BglI	GCCNNNNNGGC	2 cut(s)	461, 614
BglII	AGATCT	1 cut(s)	784
BlnI	CCTAGG	1 cut(s)	2185
BlpI	GCTNAGC	2 cut(s)	1182, 2340
BmcAI	AGTACT	4 cut(s)	2108, 2765, 3908, 4859
Bme18I	GGWCC	3 cut(s)	37, 3197, 5033
BmeT110I	CYCGRG	1 cut(s)	1485
BmgBI	CACGTC	2 cut(s)	4568, 4783
BmgT120I	GGNCC	5 cut(s)	37, 607, 3197, 4092, 5033
BmiI	GGNNCC	6 cut(s)	648, 745, 1142, 2214, 3368, 3540
BmrI	ACTGGG	1 cut(s)	3721
BmuI	ACTGGG	1 cut(s)	3721
BoxI	GACNNNNGTC	1 cut(s)	4172
BpiI	GAAGAC	3 cut(s)	1448, 2950, 4473
BplI	GAGNNNNNCTC	4 cut(s)	1092, 1124, 1302, 1334
BpmI	CTGGAG	3 cut(s)	1653, 1817, 4826
Bpu10I	CCTNAGC	5 cut(s)	333, 1767, 2712, 3378, 3831
Bpu1102I	GCTNAGC	2 cut(s)	1182, 2340
BpuEI	CTTGAG	4 cut(s)	237, 632, 1188, 4916
BpuMI	CCSGG	1 cut(s)	3344
BsaAI	YACGTR	3 cut(s)	2452, 3467, 4456
BsaBI	GATNNNNATC	2 cut(s)	1455, 2901
BsaHI	GRCGYC	1 cut(s)	131
BsaI	GGTCTC	2 cut(s)	841, 1425
BsaJI	CCNNGG	9 cut(s)	650, 1111, 2185, 2467, 2468, 2676, 3543, 3754, 4230
BsaWI	WCCGGW	2 cut(s)	3840, 4288
BsaXI	ACNNNNNCTCC	4 cut(s)	1422, 1452, 2595, 2625
Bse118I	RCCGGY	1 cut(s)	1869
Bse1I	ACTGG	9 cut(s)	431, 1341, 1394, 1670, 2260, 3716, 4118, 4707, 4843
Bse21I	CCTNAGG	1 cut(s)	1617
Bse3DI	GCAATG	2 cut(s)	1878, 2555
Bse8I	GATNNNNATC	2 cut(s)	1455, 2901
BseAI	TCCGGA	1 cut(s)	4288
BseDI	CCNNGG	9 cut(s)	650, 1111, 2185, 2467, 2468, 2676, 3543, 3754, 4230
BseGI	GGATG	8 cut(s)	1750, 1857, 2898, 2902, 3889, 4285, 4879, 4945
BseJI	GATNNNNATC	2 cut(s)	1455, 2901
BseMI	GCAATG	2 cut(s)	1878, 2555
BseNI	ACTGG	9 cut(s)	431, 1341, 1394, 1670, 2260, 3716, 4118, 4707, 4843
BseRI	GAGGAG	4 cut(s)	1191, 1263, 2603, 4829
BseSI	GKGCMC	2 cut(s)	748, 4269
BseYI	CCCAGC	1 cut(s)	704
BsgI	GTGCAG	4 cut(s)	1137, 2474, 3345, 4977
BshNI	GGYRCC	1 cut(s)	743
BsiHKAI	GWGCWC	5 cut(s)	494, 3561, 3979, 4206, 4269
BsiHKCI	CYCGRG	1 cut(s)	1485
BsiSI	CCGG	4 cut(s)	1870, 3343, 3841, 4289
BslFI	GGGAC	1 cut(s)	1309
BsmBI	CGTCTC	3 cut(s)	122, 2615, 4558
BsmFI	GGGAC	1 cut(s)	1309
BsmI	GAATGC	8 cut(s)	1418, 2479, 2516, 3023, 3338, 4739, 5070, 5094
Bso31I	GGTCTC	2 cut(s)	841, 1425
BsoBI	CYCGRG	1 cut(s)	1485
Bsp1286I	GDGCHC	7 cut(s)	494, 748, 2996, 3561, 3979, 4206, 4269
Bsp13I	TCCGGA	1 cut(s)	4288
Bsp1407I	TGTACA	3 cut(s)	1255, 4134, 4641
Bsp1720I	GCTNAGC	2 cut(s)	1182, 2340
BspEI	TCCGGA	1 cut(s)	4288
BspHI	TCATGA	2 cut(s)	3027, 4176
BspLI	GGNNCC	6 cut(s)	648, 745, 1142, 2214, 3368, 3540
BspMAI	CTGCAG	4 cut(s)	205, 2082, 3013, 3205
BspMI	ACCTGC	5 cut(s)	328, 823, 1549, 3208, 4999
BspT107I	GGYRCC	1 cut(s)	743
BspTNI	GGTCTC	2 cut(s)	841, 1425
BsrDI	GCAATG	2 cut(s)	1878, 2555
BsrFI	RCCGGY	1 cut(s)	1869
BsrGI	TGTACA	3 cut(s)	1255, 4134, 4641
BsrI	ACTGG	9 cut(s)	431, 1341, 1394, 1670, 2260, 3716, 4118, 4707, 4843
BssAI	RCCGGY	1 cut(s)	1869
BssECI	CCNNGG	9 cut(s)	650, 1111, 2185, 2467, 2468, 2676, 3543, 3754, 4230
BssNI	GRCGYC	1 cut(s)	131
BssSI	CACGAG	2 cut(s)	3047, 4341
BssT1I	CCWWGG	5 cut(s)	2185, 2676, 3543, 3754, 4230
Bst2BI	CACGAG	2 cut(s)	3047, 4341
Bst6I	CTCTTC	3 cut(s)	2588, 2803, 4926
BstACI	GRCGYC	1 cut(s)	131
BstAPI	GCANNNNNTGC	6 cut(s)	209, 623, 632, 1514, 3121, 3788
BstAUI	TGTACA	3 cut(s)	1255, 4134, 4641
BstBAI	YACGTR	3 cut(s)	2452, 3467, 4456
BstC8I	GCNNGC	8 cut(s)	688, 1080, 1188, 1773, 2098, 3324, 3820, 4597
BstF5I	GGATG	8 cut(s)	1750, 1857, 2898, 2902, 3889, 4285, 4879, 4945
BstHHI	GCGC	2 cut(s)	637, 3385
BstPAI	GACNNNNGTC	1 cut(s)	4172
BstSFI	CTRYAG	7 cut(s)	201, 281, 919, 1020, 2078, 3009, 3201
BstSLI	GKGCMC	2 cut(s)	748, 4269
BstV2I	GAAGAC	3 cut(s)	1448, 2950, 4473
BstX2I	RGATCY	7 cut(s)	234, 784, 1612, 2047, 2212, 2220, 3148
BstYI	RGATCY	7 cut(s)	234, 784, 1612, 2047, 2212, 2220, 3148
Bsu36I	CCTNAGG	1 cut(s)	1617
BtrI	CACGTC	2 cut(s)	4568, 4783
BtsCI	GGATG	8 cut(s)	1750, 1857, 2898, 2902, 3889, 4285, 4879, 4945
BtsI	GCAGTG	1 cut(s)	1423
BtsIMutI	CAGTG	9 cut(s)	151, 1236, 1334, 1423, 1689, 3103, 3412, 3793, 4111
BveI	ACCTGC	5 cut(s)	328, 823, 1549, 3208, 4999
Cac8I	GCNNGC	8 cut(s)	688, 1080, 1188, 1773, 2098, 3324, 3820, 4597
CaiI	CAGNNNCTG	1 cut(s)	209
CciI	TCATGA	2 cut(s)	3027, 4176
CfoI	GCGC	2 cut(s)	637, 3385
Cfr10I	RCCGGY	1 cut(s)	1869
Cfr13I	GGNCC	5 cut(s)	37, 607, 3197, 4092, 5033
CseI	GACGC	2 cut(s)	52, 4367
EaeI	YGGCCR	3 cut(s)	462, 1057, 1233
Eam1104I	CTCTTC	3 cut(s)	2588, 2803, 4926
EarI	CTCTTC	3 cut(s)	2588, 2803, 4926
Ecl136II	GAGCTC	1 cut(s)	3559
Eco130I	CCWWGG	5 cut(s)	2185, 2676, 3543, 3754, 4230
Eco147I	AGGCCT	2 cut(s)	193, 2535
Eco24I	GRGCYC	2 cut(s)	2996, 3561
Eco31I	GGTCTC	2 cut(s)	841, 1425
Eco32I	GATATC	1 cut(s)	4022
Eco47I	GGWCC	3 cut(s)	37, 3197, 5033
Eco53kI	GAGCTC	1 cut(s)	3559
Eco57I	CTGAAG	6 cut(s)	981, 1688, 2538, 3079, 3495, 4212
Eco81I	CCTNAGG	1 cut(s)	1617
Eco88I	CYCGRG	1 cut(s)	1485
EcoICRI	GAGCTC	1 cut(s)	3559
EcoO109I	RGGNCCY	2 cut(s)	37, 607
EcoRI	GAATTC	4 cut(s)	391, 1209, 3605, 3836
EcoRV	GATATC	1 cut(s)	4022
EcoT14I	CCWWGG	5 cut(s)	2185, 2676, 3543, 3754, 4230
EcoT22I	ATGCAT	2 cut(s)	2134, 3571
EcoT38I	GRGCYC	2 cut(s)	2996, 3561
ErhI	CCWWGG	5 cut(s)	2185, 2676, 3543, 3754, 4230
Esp3I	CGTCTC	3 cut(s)	122, 2615, 4558
FalI	AAGNNNNNCTT	4 cut(s)	3254, 3286, 4721, 4753
FaqI	GGGAC	1 cut(s)	1309
FauI	CCCGC	1 cut(s)	4588
FauNDI	CATATG	4 cut(s)	1327, 2164, 3571, 5083
FblI	GTMKAC	1 cut(s)	2969
FokI	GGATG	8 cut(s)	1757, 1864, 2905, 2909, 3896, 4272, 4886, 4952
FriOI	GRGCYC	2 cut(s)	2996, 3561
FspI	TGCGCA	1 cut(s)	636
GlaI	GCGC	2 cut(s)	636, 3384
GsaI	CCCAGC	1 cut(s)	708
GsuI	CTGGAG	3 cut(s)	1653, 1817, 4826
HapII	CCGG	4 cut(s)	1870, 3343, 3841, 4289
HgaI	GACGC	2 cut(s)	52, 4367
HhaI	GCGC	2 cut(s)	637, 3385
Hin1I	GRCGYC	1 cut(s)	131
Hin6I	GCGC	2 cut(s)	635, 3383
HinP1I	GCGC	2 cut(s)	635, 3383
HincII	GTYRAC	2 cut(s)	1675, 2062
HindII	GTYRAC	2 cut(s)	1675, 2062
HindIII	AAGCTT	3 cut(s)	963, 4082, 4892
HpaII	CCGG	4 cut(s)	1870, 3343, 3841, 4289
Hsp92I	GRCGYC	1 cut(s)	131
HspAI	GCGC	2 cut(s)	635, 3383
Kpn2I	TCCGGA	1 cut(s)	4288
LmnI	GCTCC	8 cut(s)	535, 1075, 1622, 2482, 2629, 3312, 3366, 4201
MfeI	CAATTG	5 cut(s)	411, 554, 1061, 3414, 4614
MflI	RGATCY	7 cut(s)	234, 784, 1612, 2047, 2212, 2220, 3148
MhlI	GDGCHC	7 cut(s)	494, 748, 2996, 3561, 3979, 4206, 4269
MlsI	TGGCCA	3 cut(s)	464, 1059, 1235
MluNI	TGGCCA	3 cut(s)	464, 1059, 1235
MmeI	TCCRAC	2 cut(s)	3187, 4798
Mox20I	TGGCCA	3 cut(s)	464, 1059, 1235
Mph1103I	ATGCAT	2 cut(s)	2134, 3571
MroI	TCCGGA	1 cut(s)	4288
MroXI	GAANNNNTTC	1 cut(s)	3609
MscI	TGGCCA	3 cut(s)	464, 1059, 1235
MslI	CAYNNNNRTG	7 cut(s)	26, 3614, 3645, 3734, 3878, 4175, 4341
Msp20I	TGGCCA	3 cut(s)	464, 1059, 1235
MspA1I	CMGCKG	4 cut(s)	598, 1138, 2246, 3014
MspI	CCGG	4 cut(s)	1870, 3343, 3841, 4289
MunI	CAATTG	5 cut(s)	411, 554, 1061, 3414, 4614
Mva1269I	GAATGC	8 cut(s)	1418, 2479, 2516, 3023, 3338, 4739, 5070, 5094
NciI	CCSGG	1 cut(s)	3344
NdeI	CATATG	4 cut(s)	1327, 2164, 3571, 5083
NlaIV	GGNNCC	6 cut(s)	648, 745, 1142, 2214, 3368, 3540
NmeAIII	GCCGAG	1 cut(s)	1327
NmuCI	GTSAC	7 cut(s)	132, 382, 1332, 2819, 2839, 3727, 4370
NsbI	TGCGCA	1 cut(s)	636
NsiI	ATGCAT	2 cut(s)	2134, 3571
OliI	CACNNNNGTG	1 cut(s)	3734
PaeI	GCATGC	1 cut(s)	1775
PagI	TCATGA	2 cut(s)	3027, 4176
PasI	CCCWGGG	1 cut(s)	2468
PceI	AGGCCT	2 cut(s)	193, 2535
PciI	ACATGT	4 cut(s)	3640, 4638, 4745, 4959
PcsI	WCGNNNNNNNCGW	1 cut(s)	4545
PctI	GAATGC	8 cut(s)	1418, 2479, 2516, 3023, 3338, 4739, 5070, 5094
PdmI	GAANNNNTTC	1 cut(s)	3609
PfeI	GAWTC	4 cut(s)	422, 661, 1196, 3356
PflFI	GACNNNGTC	2 cut(s)	2607, 3500
PflMI	CCANNNNNTGG	1 cut(s)	5084
PfoI	TCCNGGA	3 cut(s)	259, 1143, 1380
Ppu21I	YACGTR	3 cut(s)	2452, 3467, 4456
PpuMI	RGGWCCY	1 cut(s)	37
PscI	ACATGT	4 cut(s)	3640, 4638, 4745, 4959
PshAI	GACNNNNGTC	1 cut(s)	4172
PshBI	ATTAAT	3 cut(s)	993, 1640, 3938
PsiI	TTATAA	2 cut(s)	479, 2528
Psp124BI	GAGCTC	1 cut(s)	3561
Psp1406I	AACGTT	1 cut(s)	3624
Psp5II	RGGWCCY	1 cut(s)	37
PspFI	CCCAGC	1 cut(s)	704
PspN4I	GGNNCC	6 cut(s)	648, 745, 1142, 2214, 3368, 3540
PspPI	GGNCC	5 cut(s)	37, 607, 3197, 4092, 5033
PspPPI	RGGWCCY	1 cut(s)	37
PstI	CTGCAG	4 cut(s)	205, 2082, 3013, 3205
PstNI	CAGNNNCTG	1 cut(s)	209
PsuI	RGATCY	7 cut(s)	234, 784, 1612, 2047, 2212, 2220, 3148
PsyI	GACNNNGTC	2 cut(s)	2607, 3500
PvuII	CAGCTG	2 cut(s)	2246, 3014
RseI	CAYNNNNRTG	7 cut(s)	26, 3614, 3645, 3734, 3878, 4175, 4341
SacI	GAGCTC	1 cut(s)	3561
Sau96I	GGNCC	5 cut(s)	37, 607, 3197, 4092, 5033
ScaI	AGTACT	4 cut(s)	2108, 2765, 3908, 4859
SduI	GDGCHC	7 cut(s)	494, 748, 2996, 3561, 3979, 4206, 4269
SfcI	CTRYAG	7 cut(s)	201, 281, 919, 1020, 2078, 3009, 3201
SinI	GGWCC	3 cut(s)	37, 3197, 5033
SmiMI	CAYNNNNRTG	7 cut(s)	26, 3614, 3645, 3734, 3878, 4175, 4341
SmlI	CTYRAG	4 cut(s)	252, 611, 1203, 4895
SmoI	CTYRAG	4 cut(s)	252, 611, 1203, 4895
SpeI	ACTAGT	1 cut(s)	3400
SphI	GCATGC	1 cut(s)	1775
SseBI	AGGCCT	2 cut(s)	193, 2535
SspI	AATATT	1 cut(s)	4075
SstI	GAGCTC	1 cut(s)	3561
StuI	AGGCCT	2 cut(s)	193, 2535
StyI	CCWWGG	5 cut(s)	2185, 2676, 3543, 3754, 4230
TaqI	TCGA	7 cut(s)	230, 1980, 2580, 2602, 2922, 3348, 4539
TauI	GCSGC	3 cut(s)	8, 3046, 5106
TfiI	GAWTC	4 cut(s)	422, 661, 1196, 3356
TscAI	CASTG	9 cut(s)	151, 1236, 1341, 1423, 1696, 3103, 3412, 3793, 4118
TseFI	GTSAC	7 cut(s)	132, 382, 1332, 2819, 2839, 3727, 4370
Tsp45I	GTSAC	7 cut(s)	132, 382, 1332, 2819, 2839, 3727, 4370
TspGWI	ACGGA	6 cut(s)	385, 1595, 1709, 2597, 3141, 3419
TspRI	CASTG	9 cut(s)	151, 1236, 1341, 1423, 1696, 3103, 3412, 3793, 4118
Tth111I	GACNNNGTC	2 cut(s)	2607, 3500
Van91I	CCANNNNNTGG	1 cut(s)	5084
VneI	GTGCAC	1 cut(s)	4265
VpaK11BI	GGWCC	3 cut(s)	37, 3197, 5033
VspI	ATTAAT	3 cut(s)	993, 1640, 3938
XbaI	TCTAGA	3 cut(s)	898, 3155, 4789
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2881, 3451
XmaJI	CCTAGG	1 cut(s)	2185
XmiI	GTMKAC	1 cut(s)	2969
XmnI	GAANNNNTTC	1 cut(s)	3609
ZraI	GACGTC	1 cut(s)	132
ZrmI	AGTACT	4 cut(s)	2108, 2765, 3908, 4859
Zsp2I	ATGCAT	2 cut(s)	2134, 3571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.