Rh5DG490800

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002740
Physical Location & Seq
Forward (+)
77112884 .. 77126910
14027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG490800.1

Sequence Viewer

Length: 6318 bp
ATGGAGAATTCTCACTACAGTCAATCCCACACGGCTTCATGGATCAGAGGCAAGTGCGTAGGCAGAGGCGCGTTCGGCACGGTCAATATCGGAGTCAACAAATCGGACGGTGAAGCGTTCGCCGTCAAGTCCGTGGATCTCGCCGCGTGTCTCCCCAGCCAGGCGGAGGCGCTCGAGAACGAAATCCGGATTCTCCGGTCGCTCTCCTCGCCTTATGTGGTGAAGTACCTGAACGACGATCACACGGCGTCGTATCGGAATTTGCATATGGAGTACTTGCCGGGTGGCACCGCCGGGGACTCTGCTGACGTGGACGAGAGTGTTGTACAGTCGCGGACGTGGTGCGTGGTGTCGGCTCTCAAGTATGTACACTCCCGGGGGATTGTTCACTGCGACGTGAAAGGGAGAAATGTTCTGGTGGGGTCCGGTTCGGACCGGGCGAAGCTAGCGGATTTCGGTTCGGCCGTGGATTATATGTCGGGGGACGGGTGTAGGGGGAGGATCTCGCCACGTGGAAGCCCGCTGTGGATGGCGCCGGAGGTGATCAGAGGGGAGTATCAGGGGCCGGAGTCAGACGTGTGGTCGTTGGGGTGCACGGTGATCGAGATGGTCACCGGGAAGCCCGCGTGGGAGGACGAGGGAGTCGAGACGCTGAGCCGGATCGGGTTGTCGGTCGGGTTGCCGGAGTTTCCGACCCGGTTGTCCGAAACTGGTCGGGATTTTCTGGACAAGTGTCTGAGGAGAGACCCGGAGGAGAGGTGGAGCTGCGATCAGCTACTGCAGCACCCATTTCTGGCGTCAGTTTCGCCCAATGCGGCGGCGGATTCTTCGCCGCGGTGCGTGCTCGACTGGGTCAACTCGGAGTTCGAAGACGACGAGTACGAAAACGACGAAGAGAGTTGTTCTATTGACGAGGATTCCGCTAAGGAGCGGATTGGTAAATTGGCCTCGAACTCAGGGGTAGATTGGGAATTTTCAGACGGGTGGACGGTGGTGCGGGGCGCAGTAGAAGTAGTGGAATCCGGCGCGTTTTCGCGGGAAGAGACGAGTTCGGGATATTCAGGTTGTGAAGGGGCATTTTCGGAAGGTGAAAGTTGCGGTGGCGGTGATTTAGTGGAGTGGGCACGTAATGAGTGTGATACGGTTGGCTCAAGTGGTCGTTGGTATGGGCCGTCGGTGATGGAATATTCGGCGGTGGAGAGGAGCAGCTTCGCCAATCGGGTCGGTCCATCCCTTCGAAGCCCCGATCCAGGCGCCATGGCCGCCGCTGCCAATGCCCCCATGACCATGAAAGAGCAGCTCACGCTGCCTAGTATTGGGATTAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGCGTTAGGGAGACCTCGCCGCAGAATAGCATCGTGATCGTTGACATGAACATGCCAATGCAGCCGTTGCGACGGCCGATTACTGCTGATTCGGCTTTGATGAATCCTAATTCGAGGATTCTGGCTCTTAAAGCTCAAGTGCAAGGAACTACTCAGGATCATTTACAGATATTTAACATTGAACTGAAAACGAAGATGAAATCACATCAGATGCCTGAGCAGATTGTGTTTTGGAAGTGGGTCACTCCTAAGATGCTGGGGATGGTCACACAGACCTCAGTATACCATTGGTTAATTGATGGGGATTCTGAGCCAACAAAGGTGTTTGAGCGTACAGCTAATTTAGCAAACAATCAAATTATCAACTACCGTTGTGATCCCTCTGAAAAGTGGTTGGTCTTAATTGGAATTGCTCCTGGTTCTCCTGAGAGGCCACAACTAGTTAAAGGAAACATGCAGCTTTTCTCTGTGGAGCAACAACGTAGTCAGGCTCTTGAAGCACATGCTGCATCATTTGCCCCATTTAAAGTTGCAGGGAACGAGAACCCTTCAATTCTTATATGCTTTGCTGCAAAGTCCTCCAACGCTGGACAAGTTACATCAAAGTTGCATGTTATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATATACTAAGAAGCAAGCAGATCTATTCTTTCCTCCGGATTTTGCTGATGACTTTCCTGTGTCAATGCAGATATCAGAAAAGTATGGGTTGATATATGTTATTACAAAGCTTGGACTTCTGTTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCGGATCCTATATTTTTGACAGCTGAAGCTTCATCTGTTGGAGGTTTTTATGCAGTCAACAGGCGTGGTCAGGTGTTACTTGCCACTGTAAATGAACAAACAATTGTACCTTTTGTTAGTGGTCAGTTAAACAATTTGGGACTCGCAGTTAGTCTTGCCAGAAGAGGAAATCTTCCTGGTGCTGAAGACTTGGTTGTCCAGCGGTTCCAAGAATTGTTTTCTCAGACAAAATATAAGGAGGCTGCAGAGCTTGCTGCAGATTCTCCGATGGGAGTACTCCGTACACCTGAAACTGTTGCTAAATTTCAGGGTGTTCCTGTGCAAGCTGGGCAAACACCACCATTGTTGCAGTACTTTGGAACTTTGTTAACTAAAGGGAAACTCAACGCTTTTGAATCATTGGAACTGTCACGTTTGGTTGTAAATCAAAACAAGAAGAATCTTTTGGAGAATTGGCTGGCGGAGGACAAGCTTGAATGTAGCGAAGAGCTAGGAGATCTTGTGAAGACAGTGGATAATGACCTTGCTTTGAAAATATACATCAAAGCCAGGGCAACTCCAAAAGTTGTTGCAGCCTTTGCTGAGAGAAGGGAATTTGACAAGATTTTAATATACTCAAAACAGGTTGGATATACACCTGATTATATGTTCCTTCTGCAAACAATTTTGCGGACAGATGCCCAGGGGGCTGTCAATTTTGCACTAATGATGTCTCAAATGGAAGGAGGTTGCCCTATTGACTACAATACCATTACTGATCTGTTCCTTCAGAGGAATTTGATTCGTGAGGCAACAGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCTGAGCATGCCCACCTTCAGACAAAGGTTTTGGAAATCAATCTAGTAACTTTTCCCAATGTTGCGGATGCTATATTGACCAATGGTATGTTCAGTCATTATGACCGCCCCCGCATTGGTCAACTCTGTGAAAAAGCTGGTTTGTATTTGCGTGCCTTACAGCATTATACCGAGTTACCTGATATTAAAAGAGTCATTGTGAACACACATGCAATTGACCCTCAGGCTCTTGTTGAGTTCTTCGGGACTCTTTCCATAGAATGGGCTCTTGAGTGCATGAAAGATCTTCTACTGGTCAATCTTAGGGGCAATCTTCAGATTATTGTGCAGACTGCCAAAGAGTACTCCGAGCAACTGGGTGTAGATAAATGTATAAAGTTGTTTGAGCAGTTCAAGTCCTATGAGGGGTTGTACTTTTTCTTAGGCTCATACTTGAGCTCCAGTGAGGATCCCGATATCCACTTCAAGTATATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTAGAGCGAGTGACTCGTGAATCTAATTTTTATGATGCTGAGAAGGCTAAGAACTTTCTAATGGAAGCAAAGCTTCCAGATGCGCGTCCCCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCTGATCTCACTCATTACCTATATACGAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTTGGGCAGCTTTTGGATGATGAATGTCCTGAAGATTTCATCAAAGGACTTATTCTATCAGTTCGTTCACTTCTACCAGTCGAGCCCCTTGTTGATGAATGTGAAAAGAGGAATCGTCTTCGCCTGCTCACTCAGTTCTTGGAGCATCTTGTGAGCGAAGGAAGCCAAGATGTACATGTTCATAATGCTCTTGGTAAAATCATCATAGATAGTGGCAACAATCCAGAGCATTTTCTCACAACTAACCCATATTATGATTCTCGCGTTGTGGGTAAATATTGTGAAAAAAGAGATCCCACCCTTGCTGTTGTTGCTTACCGTAGAGGGCAATGTGATGATGAACTTGTTAATGTAACAAATAAGAATTCATTATTCAAACTCCAGGCCAGATATGTTGTGGAAAGAATGGATGATGACCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAGTATAGAAGGCAGCTAATTGATCAAGTTGTCTCTACTGCTTTGCCTGAAAGCAAGAGCCCTGAGCAAGTTTCAGCAACTGTTAAAGCGTTTATGACTGCGGACCTTCCACATGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTCCAAAATTCAGCTTTTAGTGGGAACTTCAATCTACAGAATCTGTTGATCTTGACTGCAATTAAGGCTGATCCATCCAGAGTCATGGATTATATCAATAGATTAGACAACTTTGATGGGCCTGCAGTTGGAGTAATGGCTGTAGAAGCCCAACTCTATGAGGAAGCATTTGCAATCTTTAAGAAGTTCAATTTGAATGTTGATGCTGTCAATGTTCTGTTGGATAACATTCAGAGCATTGACCGTGCCGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTTGCCAAGGCCCAACTACGAGAGGGGCTTGTGAGTGATGCTATCGAGTCATTCATTCGTGCTGATGATGCCACCCAGTTTTTGGATGTCATACGTGCTGCTCAGGATGCAGATGTGTATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGGCGAAAGAGCCCAAGGTAGACAGTGAACTCATTTATGCATATGCTAAGATTGATCGACTTGGTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCTAATCTCCCAAATGTTGGGGATCGCCTGTATGATGAAGAACTATATGAAGCAGCCAAGATCATATTTGCATTTATTTCTAATTGGGCCAAGCTGGCGTGTACTCTAGTGAAGCTCAAGCAGTTTCAAGGTGCTGTTGATGCCGCCCGGAAAGCTAACAGCTCAAAAACTTGGAAGGAAGTTTGCTTTGCATGTGTTGATGCTGAGGAATTCCGGTTGGCTCAAATTTGTGGACTCAACATCATCATCCAGGTGGATGATTTGGAAGAGGTCAGTGAGTTTTACCAGAACAGGGGTTGCTTTAATGAGCTCATTAATCTTATGGAGAGTGGACTAGGATTGGAACGTGCACATATGGGCATCTTTACTGAGTTAGGAGTTCTATATGCAAGATACCGTCCTGAGAAGCTTATGGAGCACATTAAACTGTTTTCTACTCGTCTCAACATTCCCAAACTTATACGAGCTTGTGATGAACAGCAGCACTGGAAGGAATTAACATACTTATATATACAATACGATGAATTTGATAATGCTGCAACCACCATAATGAACCACTCCCCAGAAGCATGGGAACACATGCAATTCAAGGATGTGATAGTCAAAGTTGCAAGTGTTGAGCTTTACTATAGGGCTGTGCACTTTTACTTAGAAGAACATCCTGATATTATCAATGATGTTCTAAATGTGCTTGCGCTTCGTGTGGATCATACCCGAGTGGTGGATATAATGCGAAAGGCTGGTCACGTCCGTCTGGTAAAGCCATACATGGTTGCAGTCCAGAGCAGCAATGTAACAGCTGTAAATGAAGCTCTAAATGAAATATATATTGAGGAGGAAGACTATGACAGACTTCGTGAATCTATAGATTTGCATGATAGCTTTGACCAGATAGGTCTTGCACAAAAGCTTGAGAAACATGAGCTTCTTGAGATGAGACGTGTTGCTGCATACATTTATAAGAAAGCTGGAAGATGGAAGCAATCCATTGCATTGTCAAAGAAAGACAAACATTACAAAGACGCCATGGAGACATGCTCTCAGTCTGGTGATCATGAACTTTCAGAGGAATTGCTTGTCTATTTCATCGAGCAGGGAAAGAAAGAATGTTTCGCATCTTGTCTCTTTGTTTGTTATGATCTAGTCCGGCCAGACGTTGCTCTTGAGCTGGCATGGATGAACAATATAATTGATTTTGCATTCCCATATATCCTGCAGTTTATTCGTGAGTACACAGGCAAGGTTGATGAACTCGTCAAAGACAAAATTGAGACTCAGAAAGAGGTGAAAGCCAAAGAGCAAGAAGAGAAGGAATTGGTTGCTAAGCAGGTATTGCTAGTTGCTTTATCCTTTTGTATCATTGATGGCCTGTGTCATACATTTGTTGTCTCCAAGAACTATAATTCGTCTGCAAGACTGCTTGGTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

2105

Amino Acids

236.9

Weight (kDa)

5.09

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 17 - 265 9e-55 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 261 3.6e-26 Protein tyrosine and serine/threonine kinase
Clathrin_propel PF01394 440 - 476 3.1e-06 Clathrin propeller repeat
Clathrin-link PF09268 764 - 786 7.8e-08 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 789 - 854 1.5e-26 Clathrin-H-link
Clathrin PF00637 977 - 1112 5.7e-25 Region in Clathrin and VPS
Clathrin PF00637 1121 - 1261 9.3e-27 Region in Clathrin and VPS
Clathrin PF00637 1270 - 1404 3e-32 Region in Clathrin and VPS
Clathrin PF00637 1413 - 1551 3.9e-32 Region in Clathrin and VPS
Clathrin PF00637 1564 - 1701 8.9e-30 Region in Clathrin and VPS
Clathrin PF00637 1710 - 1851 1e-25 Region in Clathrin and VPS
Clathrin PF00637 1858 - 1999 1.8e-31 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5847
AasI GACNNNNNNGTC 3 cut(s) 641, 700, 2390
Acc36I ACCTGC 1 cut(s) 6205
Acc65I GGTACC 1 cut(s) 4767
AccB1I GGYRCC 5 cut(s) 287, 532, 1253, 2003, 4767
AccB7I CCANNNNNTGG 3 cut(s) 3285, 4714, 4904
AccBSI CCGCTC 1 cut(s) 931
AccI GTMKAC 2 cut(s) 1659, 4809
AccII CGCG 9 cut(s) 71, 146, 334, 626, 835, 1028, 1036, 3616, 4011
AccIII TCCGGA 3 cut(s) 186, 2059, 2197
AcoI YGGCCR 4 cut(s) 462, 1260, 1451, 6035
AcuI CTGAAG 7 cut(s) 2241, 2400, 2948, 3026, 3032, 3323, 3798
AcvI CACGTG 1 cut(s) 512
AcyI GRCGYC 5 cut(s) 248, 533, 797, 1254, 5910
AflIII ACRYGT 3 cut(s) 576, 3922, 5827
AhdI GACNNNNNGTC 1 cut(s) 580
AhlI ACTAGT 1 cut(s) 1816
AjiI CACGTC 6 cut(s) 310, 339, 397, 577, 5635, 5828
AjuI GAANNNNNNNTTGG 4 cut(s) 2624, 2656, 3098, 3130
Alw21I GWGCWC 8 cut(s) 596, 846, 3464, 4341, 5199, 5239, 5307, 5529
Alw44I GTGCAC 3 cut(s) 592, 5235, 5525
AlwNI CAGNNNCTG 4 cut(s) 778, 2171, 4274, 4381
Ama87I CYCGRG 3 cut(s) 173, 375, 5601
Aor13HI TCCGGA 3 cut(s) 186, 2059, 2197
ApaLI GTGCAC 3 cut(s) 592, 5235, 5525
ArsI GACNNNNNNTTYG 4 cut(s) 3037, 3069, 5614, 5646
AseI ATTAAT 3 cut(s) 1323, 3627, 5202
Asp718I GGTACC 1 cut(s) 4767
AspLEI GCGC 8 cut(s) 71, 172, 535, 1004, 1028, 1256, 3616, 5584
AsuII TTCGAA 2 cut(s) 867, 1237
AsuNHI GCTAGC 1 cut(s) 445
AvaI CYCGRG 3 cut(s) 173, 375, 5601
AvaII GGWCC 5 cut(s) 423, 433, 1226, 4177, 4297
AxyI CCTNAGG 1 cut(s) 3246
BaeGI GKGCMC 5 cut(s) 596, 1126, 2008, 5239, 5529
BaeI ACNNNNGTAYC 2 cut(s) 2286, 2319
BamHI GGATCC 2 cut(s) 2200, 3472
BanI GGYRCC 5 cut(s) 287, 532, 1253, 2003, 4767
BanII GRGCYC 6 cut(s) 3292, 3464, 3834, 4256, 4803, 5199
BarI GAAGNNNNNNTAC 2 cut(s) 4755, 4787
BauI CACGAG 1 cut(s) 3546
BbrPI CACGTG 1 cut(s) 512
BbsI GAAGAC 5 cut(s) 876, 2388, 2708, 3857, 5733
Bbv12I GWGCWC 8 cut(s) 596, 846, 3464, 4341, 5199, 5239, 5307, 5529
BbvCI CCTCAGC 1 cut(s) 5091
BceAI ACGGC 8 cut(s) 48, 107, 261, 449, 1156, 1426, 1466, 4571
BcgI CGANNNNNNTGC 8 cut(s) 583, 617, 1396, 1430, 3670, 3704, 6092, 6126
BclI TGATCA 3 cut(s) 543, 4216, 5938
BcuI ACTAGT 1 cut(s) 1816
BfoI RGCGCY 3 cut(s) 173, 536, 1257
BfuAI ACCTGC 1 cut(s) 6205
BglI GCCNNNNNGGC 2 cut(s) 2882, 4982
BglII AGATCT 3 cut(s) 2044, 2692, 3307
BlpI GCTNAGC 2 cut(s) 653, 6210
BmcAI AGTACT 4 cut(s) 275, 2472, 2549, 3368
Bme18I GGWCC 5 cut(s) 423, 433, 1226, 4177, 4297
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 3 cut(s) 173, 375, 5601
BmgBI CACGTC 6 cut(s) 310, 339, 397, 577, 5635, 5828
BmrI ACTGGG 3 cut(s) 859, 3389, 4702
BmtI GCTAGC 1 cut(s) 449
BmuI ACTGGG 3 cut(s) 859, 3389, 4702
BoxI GACNNNNGTC 1 cut(s) 732
BpiI GAAGAC 5 cut(s) 876, 2388, 2708, 3857, 5733
BplI GAGNNNNNCTC 2 cut(s) 5909, 5941
BpmI CTGGAG 2 cut(s) 3448, 4112
Bpu10I CCTNAGC 7 cut(s) 924, 1593, 3027, 4173, 4257, 4734, 5091
Bpu1102I GCTNAGC 2 cut(s) 653, 6210
Bpu14I TTCGAA 2 cut(s) 867, 1237
BpuEI CTTGAG 9 cut(s) 344, 1135, 1497, 3314, 3478, 4988, 5819, 5837, 6071
BsaAI YACGTR 3 cut(s) 512, 1127, 4727
BsaBI GATNNNNATC 1 cut(s) 4749
BsaHI GRCGYC 5 cut(s) 248, 533, 797, 1254, 5910
BsaI GGTCTC 2 cut(s) 738, 1382
BsaWI WCCGGW 6 cut(s) 186, 195, 425, 2059, 2197, 5100
BsaXI ACNNNNNCTCC 6 cut(s) 743, 773, 2682, 2712, 3446, 3476
Bse1I ACTGG 9 cut(s) 715, 854, 3321, 3384, 3465, 3520, 3824, 4708, 5378
Bse21I CCTNAGG 1 cut(s) 3246
Bse3DI GCAATG 3 cut(s) 4082, 5683, 5874
Bse8I GATNNNNATC 1 cut(s) 4749
BseAI TCCGGA 3 cut(s) 186, 2059, 2197
BseJI GATNNNNATC 1 cut(s) 4749
BseMI GCAATG 3 cut(s) 4082, 5683, 5874
BseNI ACTGG 9 cut(s) 715, 854, 3321, 3384, 3465, 3520, 3824, 4708, 5378
BseRI GAGGAG 5 cut(s) 196, 754, 767, 1216, 5735
BseSI GKGCMC 5 cut(s) 596, 1126, 2008, 5239, 5529
BseX3I CGGCCG 2 cut(s) 462, 1451
BseYI CCCAGC 4 cut(s) 155, 1633, 2007, 2522
BsgI GTGCAG 1 cut(s) 3371
Bsh1236I CGCG 9 cut(s) 71, 146, 334, 626, 835, 1028, 1036, 3616, 4011
Bsh1285I CGRYCG 4 cut(s) 200, 465, 675, 1454
BshNI GGYRCC 5 cut(s) 287, 532, 1253, 2003, 4767
BsiEI CGRYCG 4 cut(s) 200, 465, 675, 1454
BsiHKAI GWGCWC 8 cut(s) 596, 846, 3464, 4341, 5199, 5239, 5307, 5529
BsiHKCI CYCGRG 3 cut(s) 173, 375, 5601
BslFI GGGAC 5 cut(s) 311, 497, 2349, 3283, 3603
BsmBI CGTCTC 4 cut(s) 641, 1037, 5333, 5818
BsmFI GGGAC 5 cut(s) 311, 497, 2349, 3283, 3603
BsmI GAATGC 2 cut(s) 4598, 6086
Bso31I GGTCTC 2 cut(s) 738, 1382
BsoBI CYCGRG 3 cut(s) 173, 375, 5601
Bsp119I TTCGAA 2 cut(s) 867, 1237
Bsp13I TCCGGA 3 cut(s) 186, 2059, 2197
Bsp1407I TGTACA 3 cut(s) 325, 367, 3919
Bsp1720I GCTNAGC 2 cut(s) 653, 6210
Bsp19I CCATGG 2 cut(s) 1257, 5913
BspEI TCCGGA 3 cut(s) 186, 2059, 2197
BspFNI CGCG 9 cut(s) 71, 146, 334, 626, 835, 1028, 1036, 3616, 4011
BspHI TCATGA 2 cut(s) 4753, 5941
BspMAI CTGCAG 6 cut(s) 783, 2176, 2443, 2455, 4465, 6105
BspMI ACCTGC 1 cut(s) 6205
BspOI GCTAGC 1 cut(s) 449
BspQI GCTCTTC 1 cut(s) 2676
BspT104I TTCGAA 2 cut(s) 867, 1237
BspT107I GGYRCC 5 cut(s) 287, 532, 1253, 2003, 4767
BspTNI GGTCTC 2 cut(s) 738, 1382
BsrBI CCGCTC 1 cut(s) 931
BsrDI GCAATG 3 cut(s) 4082, 5683, 5874
BsrGI TGTACA 3 cut(s) 325, 367, 3919
BsrI ACTGG 9 cut(s) 715, 854, 3321, 3384, 3465, 3520, 3824, 4708, 5378
BssNAI GTATAC 1 cut(s) 1660
BssNI GRCGYC 5 cut(s) 248, 533, 797, 1254, 5910
BssSI CACGAG 1 cut(s) 3546
BssT1I CCWWGG 4 cut(s) 1257, 4638, 4803, 5913
Bst1107I GTATAC 1 cut(s) 1660
Bst2BI CACGAG 1 cut(s) 3546
Bst6I CTCTTC 7 cut(s) 888, 1035, 2353, 2676, 4857, 5148, 6186
BstACI GRCGYC 5 cut(s) 248, 533, 797, 1254, 5910
BstAPI GCANNNNNTGC 6 cut(s) 1444, 1883, 1892, 2447, 2774, 6220
BstAUI TGTACA 3 cut(s) 325, 367, 3919
BstBAI YACGTR 3 cut(s) 512, 1127, 4727
BstBI TTCGAA 2 cut(s) 867, 1237
BstDSI CCRYGG 5 cut(s) 132, 465, 833, 1257, 5913
BstEII GGTNACC 1 cut(s) 610
BstFNI CGCG 9 cut(s) 71, 146, 334, 626, 835, 1028, 1036, 3616, 4011
BstH2I RGCGCY 3 cut(s) 173, 536, 1257
BstHHI GCGC 8 cut(s) 71, 172, 535, 1004, 1028, 1256, 3616, 5584
BstMCI CGRYCG 4 cut(s) 200, 465, 675, 1454
BstPAI GACNNNNGTC 1 cut(s) 732
BstPI GGTNACC 1 cut(s) 610
BstSLI GKGCMC 5 cut(s) 596, 1126, 2008, 5239, 5529
BstUI CGCG 9 cut(s) 71, 146, 334, 626, 835, 1028, 1036, 3616, 4011
BstV2I GAAGAC 5 cut(s) 876, 2388, 2708, 3857, 5733
BstX2I RGATCY 8 cut(s) 136, 501, 2044, 2200, 2692, 3307, 3472, 4039
BstXI CCANNNNNNTGG 2 cut(s) 4423, 5457
BstYI RGATCY 8 cut(s) 136, 501, 2044, 2200, 2692, 3307, 3472, 4039
BstZ17I GTATAC 1 cut(s) 1660
BstZI CGGCCG 2 cut(s) 462, 1451
Bsu36I CCTNAGG 1 cut(s) 3246
BtgI CCRYGG 5 cut(s) 132, 465, 833, 1257, 5913
BtrI CACGTC 6 cut(s) 310, 339, 397, 577, 5635, 5828
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 7 cut(s) 388, 2280, 2712, 3472, 4819, 5167, 5371
BveI ACCTGC 1 cut(s) 6205
CaiI CAGNNNCTG 4 cut(s) 778, 2171, 4274, 4381
CciI TCATGA 2 cut(s) 4753, 5941
CfoI GCGC 8 cut(s) 71, 172, 535, 1004, 1028, 1256, 3616, 5584
Cfr42I CCGCGG 1 cut(s) 836
Cfr9I CCCGGG 1 cut(s) 375
CpoI CGGWCCG 1 cut(s) 433
CseI GACGC 5 cut(s) 237, 658, 786, 3605, 5918
CspCI CAANNNNNGTGG 6 cut(s) 2242, 2277, 2523, 2558, 4293, 4328
CspI CGGWCCG 1 cut(s) 433
DinI GGCGCC 2 cut(s) 534, 1255
DraI TTTAAA 1 cut(s) 1903
DrdI GACNNNNNNGTC 3 cut(s) 641, 700, 2390
DriI GACNNNNNGTC 1 cut(s) 580
DseDI GACNNNNNNGTC 3 cut(s) 641, 700, 2390
EaeI YGGCCR 4 cut(s) 462, 1260, 1451, 6035
EagI CGGCCG 2 cut(s) 462, 1451
Eam1104I CTCTTC 7 cut(s) 888, 1035, 2353, 2676, 4857, 5148, 6186
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 7 cut(s) 888, 1035, 2353, 2676, 4857, 5148, 6186
EciI GGCGGA 3 cut(s) 179, 836, 2672
Ecl136II GAGCTC 2 cut(s) 3462, 5197
EclXI CGGCCG 2 cut(s) 462, 1451
Eco130I CCWWGG 4 cut(s) 1257, 4638, 4803, 5913
Eco24I GRGCYC 6 cut(s) 3292, 3464, 3834, 4256, 4803, 5199
Eco31I GGTCTC 2 cut(s) 738, 1382
Eco32I GATATC 2 cut(s) 2097, 3481
Eco47I GGWCC 5 cut(s) 423, 433, 1226, 4177, 4297
Eco52I CGGCCG 2 cut(s) 462, 1451
Eco53kI GAGCTC 2 cut(s) 3462, 5197
Eco57I CTGAAG 7 cut(s) 2241, 2400, 2948, 3026, 3032, 3323, 3798
Eco72I CACGTG 1 cut(s) 512
Eco81I CCTNAGG 1 cut(s) 3246
Eco88I CYCGRG 3 cut(s) 173, 375, 5601
Eco91I GGTNACC 1 cut(s) 610
EcoICRI GAGCTC 2 cut(s) 3462, 5197
EcoO109I RGGNCCY 1 cut(s) 4177
EcoO65I GGTNACC 1 cut(s) 610
EcoRI GAATTC 3 cut(s) 7, 4111, 5096
EcoRV GATATC 2 cut(s) 2097, 3481
EcoT14I CCWWGG 4 cut(s) 1257, 4638, 4803, 5913
EcoT22I ATGCAT 1 cut(s) 4831
EcoT38I GRGCYC 6 cut(s) 3292, 3464, 3834, 4256, 4803, 5199
EgeI GGCGCC 2 cut(s) 534, 1255
EheI GGCGCC 2 cut(s) 534, 1255
ErhI CCWWGG 4 cut(s) 1257, 4638, 4803, 5913
Esp3I CGTCTC 4 cut(s) 641, 1037, 5333, 5818
FalI AAGNNNNNCTT 2 cut(s) 3588, 3620
FaqI GGGAC 5 cut(s) 311, 497, 2349, 3283, 3603
FauI CCCGC 5 cut(s) 528, 631, 990, 1029, 3143
FauNDI CATATG 3 cut(s) 267, 4831, 5241
FbaI TGATCA 3 cut(s) 543, 4216, 5938
FblI GTMKAC 2 cut(s) 1659, 4809
FriOI GRGCYC 6 cut(s) 3292, 3464, 3834, 4256, 4803, 5199
GlaI GCGC 8 cut(s) 70, 171, 534, 1003, 1027, 1255, 3615, 5583
GsaI CCCAGC 4 cut(s) 159, 1637, 2011, 2526
GsuI CTGGAG 2 cut(s) 3448, 4112
HaeII RGCGCY 3 cut(s) 173, 536, 1257
HgaI GACGC 5 cut(s) 237, 658, 786, 3605, 5918
HhaI GCGC 8 cut(s) 71, 172, 535, 1004, 1028, 1256, 3616, 5584
Hin1I GRCGYC 5 cut(s) 248, 533, 797, 1254, 5910
Hin6I GCGC 8 cut(s) 69, 170, 533, 1002, 1026, 1254, 3614, 5582
HinP1I GCGC 8 cut(s) 69, 170, 533, 1002, 1026, 1254, 3614, 5582
HincII GTYRAC 6 cut(s) 97, 856, 1420, 2254, 2565, 3146
HindII GTYRAC 6 cut(s) 97, 856, 1420, 2254, 2565, 3146
HindIII AAGCTT 6 cut(s) 2132, 2223, 2666, 3602, 5294, 5795
HpaI GTTAAC 1 cut(s) 2565
Hpy99I CGWCG 7 cut(s) 239, 253, 398, 878, 893, 1177, 1452
Hsp92I GRCGYC 5 cut(s) 248, 533, 797, 1254, 5910
HspAI GCGC 8 cut(s) 69, 170, 533, 1002, 1026, 1254, 3614, 5582
KasI GGCGCC 2 cut(s) 532, 1253
Kpn2I TCCGGA 3 cut(s) 186, 2059, 2197
KpnI GGTACC 1 cut(s) 4771
Ksp22I TGATCA 3 cut(s) 543, 4216, 5938
KspAI GTTAAC 1 cut(s) 2565
KspI CCGCGG 1 cut(s) 836
LguI GCTCTTC 1 cut(s) 2676
MbiI CCGCTC 1 cut(s) 931
MfeI CAATTG 2 cut(s) 2298, 3237
MflI RGATCY 8 cut(s) 136, 501, 2044, 2200, 2692, 3307, 3472, 4039
Mly113I GGCGCC 2 cut(s) 533, 1254
MmeI TCCRAC 7 cut(s) 716, 1983, 2215, 2803, 2982, 4447, 4539
Mph1103I ATGCAT 1 cut(s) 4831
MroI TCCGGA 3 cut(s) 186, 2059, 2197
MslI CAYNNNNRTG 6 cut(s) 1286, 1427, 1433, 4874, 5138, 5435
MspA1I CMGCKG 7 cut(s) 523, 835, 1268, 2219, 2398, 3506, 5687
MunI CAATTG 2 cut(s) 2298, 3237
Mva1269I GAATGC 2 cut(s) 4598, 6086
MvnI CGCG 9 cut(s) 71, 146, 334, 626, 835, 1028, 1036, 3616, 4011
NarI GGCGCC 2 cut(s) 533, 1254
NcoI CCATGG 2 cut(s) 1257, 5913
NdeI CATATG 3 cut(s) 267, 4831, 5241
NheI GCTAGC 1 cut(s) 445
NmuCI GTSAC 8 cut(s) 610, 1351, 1618, 1642, 2604, 3541, 4853, 5630
NsiI ATGCAT 1 cut(s) 4831
NspV TTCGAA 2 cut(s) 867, 1237
PaeI GCATGC 1 cut(s) 3035
PaeR7I CTCGAG 1 cut(s) 173
PagI TCATGA 2 cut(s) 4753, 5941
PasI CCCWGGG 1 cut(s) 2878
PciI ACATGT 1 cut(s) 3922
PciSI GCTCTTC 1 cut(s) 2676
PcsI WCGNNNNNNNCGW 6 cut(s) 437, 462, 642, 873, 879, 888
PctI GAATGC 2 cut(s) 4598, 6086
PflFI GACNNNGTC 1 cut(s) 851
PflMI CCANNNNNTGG 3 cut(s) 3285, 4714, 4904
PfoI TCCNGGA 1 cut(s) 3727
PluTI GGCGCC 2 cut(s) 536, 1257
PmaCI CACGTG 1 cut(s) 512
PmlI CACGTG 1 cut(s) 512
Ppu21I YACGTR 3 cut(s) 512, 1127, 4727
PpuMI RGGWCCY 1 cut(s) 4177
PscI ACATGT 1 cut(s) 3922
PshAI GACNNNNGTC 1 cut(s) 732
PshBI ATTAAT 3 cut(s) 1323, 3627, 5202
PsiI TTATAA 1 cut(s) 5847
Psp124BI GAGCTC 2 cut(s) 3464, 5199
Psp5II RGGWCCY 1 cut(s) 4177
PspCI CACGTG 1 cut(s) 512
PspEI GGTNACC 1 cut(s) 610
PspFI CCCAGC 4 cut(s) 155, 1633, 2007, 2522
PspPPI RGGWCCY 1 cut(s) 4177
PstI CTGCAG 6 cut(s) 783, 2176, 2443, 2455, 4465, 6105
PstNI CAGNNNCTG 4 cut(s) 778, 2171, 4274, 4381
PsuI RGATCY 8 cut(s) 136, 501, 2044, 2200, 2692, 3307, 3472, 4039
PsyI GACNNNGTC 1 cut(s) 851
PvuII CAGCTG 3 cut(s) 2219, 3506, 5687
RseI CAYNNNNRTG 6 cut(s) 1286, 1427, 1433, 4874, 5138, 5435
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SacI GAGCTC 2 cut(s) 3464, 5199
SacII CCGCGG 1 cut(s) 836
SapI GCTCTTC 1 cut(s) 2676
ScaI AGTACT 4 cut(s) 275, 2472, 2549, 3368
SfoI GGCGCC 2 cut(s) 534, 1255
Sfr274I CTCGAG 1 cut(s) 173
Sfr303I CCGCGG 1 cut(s) 836
SfuI TTCGAA 2 cut(s) 867, 1237
SgrBI CCGCGG 1 cut(s) 836
SinI GGWCC 5 cut(s) 423, 433, 1226, 4177, 4297
SlaI CTCGAG 1 cut(s) 173
SmaI CCCGGG 1 cut(s) 377
SmiMI CAYNNNNRTG 6 cut(s) 1286, 1427, 1433, 4874, 5138, 5435
SpeI ACTAGT 1 cut(s) 1816
SphI GCATGC 1 cut(s) 3035
SspDI GGCGCC 2 cut(s) 532, 1253
SspI AATATT 2 cut(s) 1187, 4025
SstI GAGCTC 2 cut(s) 3464, 5199
StyI CCWWGG 4 cut(s) 1257, 4638, 4803, 5913
TaqII GACCGA 2 cut(s) 661, 1214
TauI GCSGC 8 cut(s) 146, 818, 821, 835, 1265, 1268, 1399, 5033
TscAI CASTG 7 cut(s) 395, 2287, 2712, 3472, 4819, 5167, 5378
TseFI GTSAC 8 cut(s) 610, 1351, 1618, 1642, 2604, 3541, 4853, 5630
Tsp45I GTSAC 8 cut(s) 610, 1351, 1618, 1642, 2604, 3541, 4853, 5630
TspGWI ACGGA 3 cut(s) 121, 2465, 5627
TspMI CCCGGG 1 cut(s) 375
TspRI CASTG 7 cut(s) 395, 2287, 2712, 3472, 4819, 5167, 5378
Tth111I GACNNNGTC 1 cut(s) 851
Van91I CCANNNNNTGG 3 cut(s) 3285, 4714, 4904
VneI GTGCAC 3 cut(s) 592, 5235, 5525
VpaK11BI GGWCC 5 cut(s) 423, 433, 1226, 4177, 4297
VspI ATTAAT 3 cut(s) 1323, 3627, 5202
XcmI CCANNNNNNNNNTGG 2 cut(s) 4141, 4711
XhoI CTCGAG 1 cut(s) 173
XmaI CCCGGG 1 cut(s) 375
XmiI GTMKAC 2 cut(s) 1659, 4809
ZrmI AGTACT 4 cut(s) 275, 2472, 2549, 3368
Zsp2I ATGCAT 1 cut(s) 4831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.