Rw1G032230

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002740
Physical Location & Seq
Forward (+)
60558977 .. 60568618
9642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032230.1

Sequence Viewer

Length: 5103 bp
ATGGCGGCGGCTAACGCTCCGATCACCATGAAGGAGGCCTTAACGCTTCCGAGCGTTGGGATTAATCCGCAATTCATAACGTTTACACATGTTACTATGGAGTCCGATAAGTACATATGTGTTCGGGAAACAGCGCCGCAGAATAGTATTGTGATTATTGATATGAGTATGCCGAATCAACCTTTGAGGAGGCCTATCACCGCCGACTCTGCACTTATGAATCCAAACTCCAAAATCCTTGCTTTGAAAGCTCAAGTCCAGGGAACTACTCAGGACCACCTTCAAATTTTTAACATTGAGATGAAAGCAAAACTGAAATCACACCTTATGCCTGAGCAGATTGTCTTTTGGAAATGGATAACCCCAAAGATGTTGGGTCTTGTCACACAGACTACAGTATATCATTGGTCAATTGAAGGTGAGTCTGAACCAGTAAAGGTATTTGAGCGAACAGCTAACCTGGCAAGCAATCAGATCATAAATTACCGTTGTGATCCTTCTGAGAAGTGGTTGGTTCTGATTGGAATTGCTCCTGGTGCTCCTGAGAGGCCGCAATTGGTTAAAGGGAACTTGCAACTCTTCTCGGTGGATCAGCAGCGCAGTCAAGCTCTTGAAGCGCATGCCGCATCCTTTGCCCAGTACAAGGTTCCAGGGAATGAGAATCCTTCCATTCTGATTTCCTTTGCCACTAAGACACTCAATGCTGGACAGATTACATCCAAGTTACATGTTATTGAGCTTGGTGCCCAGCCAGGGAAGCCATCTTTTACAAAGAAACAAGCAGATCTTTTCTTTCCTCCAGATTTTGCAGATGATTTCCCAGTTGCCATGCAGATGTCTCACAAGTACAGTTTGATTTATGTAATTACAAAGCTGGGGCTTCTATTTGTATACGACTTAGAGACAGCTAGTGCAGTTTACAGAAATAGAATTAGTCCAGATCCAATTTTCTTGACGACAGAAGCTTCATCAGTGGGAGGGTTTTATGCTGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACCATTAATGAGCAAACAATTGTGCCTTTTGTCAGTGGTCAGTTAAACAATTTGGAGCTTGCCGTCAATCTGGCAAAAAGAGGAAATCTTCCTGGTGCTGAGAATCTGGTTGTCCAGCGTTTCCAAGAACTGTTTGCCCAAACCAAGTACAAAGAAGCTGCTGAACTGGCTGCTGAGTCCCCGCAAGGAATTCTTCGTACACCGGATACAGTTGCTAAATTTCAGTGTTCCTGTCCAGCTGGGCAAACACCCCCTTTGTTGCAATACTTTGGGACTCTCTTAACGAGAGGGAAGCTCAATGCCTTTGAGTCATTGGAATTGTCACGTCTTGTTGTGAACCAGAACAAGAAGAATCTTTTGGAAAACTGGTTGGCAGAGGACAAACTGGAATGCAGTGAGGAACTAGGAGATCTTGTGAAGACTGTGGATAATGATCTGGCTTTGAAAATATACATTAAAGCCAGAGCAACTCCAAAAGTTGTTGCAGCTTTTGCCGAGCGAAGGGAGTTTGACAAAATCCTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTATCTGTTCCTTTTGCAAACAATTCTCCGAGCAGACCCGCAGGGTGCTGTTAATTTTGCATTGATGATGTCCCAAATGGAGGGTGGTTGTCCTGTTGATTACAACACCATTACAGATCTCTTTCTTCAGAGGAATCTGATTCGTGAGGCAACCGCTTTTCTACTGGATGTTTTGAAGCCAAATCTCCCTGAACATGCCTTCCTGCAGACAAAGGTCCTCGAGATTAACTTGGTTACTTTTCCAAATGTGGCTGATGCCATTTTAGCCAATGGTATGTTCAGTCATTATGATCGTCCTCGTATTGCCCAGTTATGTGAAAAAGCTGGTCTTTATGTGCGAGCTTTGCAACACTACTCCGAGTTGCCTGATATTAAACGTGTCATAGTGAATACACATGCAATAGAGCCGCAGTCACTAGTAGAGTTTTTTGGTACCTTGTCAAAGGAATGGGCGTTGGAGTGTATGAAGGATCTCTTGCTGGTCAATTTGAGAGGCAACCTTCAGATAATTGTGCAGGTTGCTAAGGAGTACTCTGATCAGTTGGGTACTGATCAATGCATGAAGCTATTTGAGCAGTTCAAGTCATATGAAGGATTGTACTTCTTCCTCGGATCATTTTTGAGTTCAAGTGAGGATCCTGAAATCCACTTCAAGTACATTGAGGCAGCTGCCAAAACTGGACAAATTAAAGAGGTTGAGAGAGTTACTAGAGAATCAAATTTCTATGATGCAGAGAAAACCAAGAACTTTTTAATGGAAGCTAAGCTCCCTGATGCACGACCCCTTATCAATGTTTGCGACCGTTTTGGTTTTGTTCCAGATCTCACCCACTACTTGTACACAAACAATATGCTCCGTTACATTGAGGGTTACGTTCAAAAGGTGAATCCTTCAAATGCTCCTTTAGTTGTGGGACAGTTGCTAGATGACGAGTGCCCTGAAGATTTCATCAAAGGCTTGATCCTTTCTGTACGTTCATTGCTTCCAGTGGAGCCCCTTGTGGAGGAATGTGAGAAGAGGAATCGACTTCGTTTACTCACTCAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAGGATGTGCACGTCCACAATGCGTTGGGTAAAATCATCATTGATAGTGGAAACAATCCTGAACACTTCCTTACCACAAACCCATATTATGATTCTCGTGTTGTGGGTAAATATTGTGAGAAACGTGATCCTACTCTGGCTGTTGTTGCTTATCGACGTGGGCAATGCGATGATGAGCTTATCAATGTTACAAATAAAAACTCTTTGTTCAAATTACAAGCAAGATATGTCGTTGAGAGGATGGATGAGGATCTTTGGGCTAAAGTTCTTGACCCTGAGAATGAATTTAGAAGACAACTTATCGATCAAGTGGTTTCTACTGCCTTGCCTGAAAGCAAGAGCCCAGAACAAGTTTCTGCGGCTGTTAAGGCTTTCATGACTGCTGATTTGCCCCATGAATTAATTGAGCTTCTTGAAAAGATTGTGCTTCAAAACTCTGCCTTCAGTGGGAACTTTAATCTGCAAAATCTGCTCATTTTGACAGCCATCAAGGCAGATCCGTCCAGAGTTATGGATTATATTAATAGGCTAGACAATTTCGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCCCAACTGTATGAGGAAGCATTTGCAATTTTCAAGAAGTTCAACTTGAATGTCCAAGCTGTCAATGTCTTGCTGGATAATATCCGAAGCATTGATAGGGCTGTAGAATTTGCATTCCGTGTTGAAGAGGATGCTGTTTGGAGTCAGGTTGGAAAGGCTCAACTCCGGGAGGGACTTGTGAGTGATGCGATTGAGTCATTTATTAGGGCGGATGATGCCACACAATTCTTGGATGTTATTCGAGCTTCTGAAGATGCGGATGTTTACCATGACTTGGTGAGATACCTTCTGATGGTTAGGCAGAAGACAAAAGAGCCGAAGGTGGACAGTGAGCTCATTTATGCATATGCCAAGATTGATAGGCTGGCTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCCAATCTTCAAAATGTTGGTGATCGCCTATATGATGAGACCCTCTATGAGGCTGCAAAAATAATTTATGCTTTTATATCCAACTGGGCCAAGTTGGCTGTCACACTTGTCAAGCTCAAACAATTCCAAGGTGCTGTTGATGCAGCACGAAAAGCCAATAGTGCAAAGACATGGAAGGAAGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGCTTAGCTCAAATTTGTGGTCTCAACATCATTGTACAGGTGGATGATTTGGAGGAGGTCAGTGAATTCTACCAGAATAGAGGGTGCTTCAATGAGCTAATATCTCTAATGGAAAGTGGTCTTGGCTTAGAACGTGCACACATGGGAATCTTTACTGAATTGGGGGTTCTGTATGCTAGATACCGTCCTGAGAAACTTATGGAGCACATCAAACTGTTCTCAACTCGTTTAAATATTCCCAAGCTCATTCGAGCTTGTGATGAGCAGCAGCATTGGAAGGAGCTAACCTATCTATATATTCAATATGATGAGTTTGATAATGCTGCAACAACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAGTTCAAAGATGTGGCAGTTAAAGTTGCAAATGTGGAGCTATATTATAAGGCTGTGCACTTTTACTTGCAAGAGCATCCAGATCTCATTAATGATATTCTAAATGTGCTTGCTCTCCGTGTAGATCATACACGTGTGGTGGACATTATGCGAAAGGCTGGGCACCTACTCCTAGTGAAGCCATACATGGTTGCTGTTCAGAGCAACAATGTCTCAGCTGTGAATGAGGCTTTGAATGAGATATATGTTGAAGAGGAAGACTATGAAAGATTGCGTGAATCAATTGATTTACATGACAGCTTTGATCAAATAGGTCTTGCTCAGAAGATTGAAAAACACGAGCTTCTGGAAATGAGGCGCGTTGCTGCTTATATTTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGCATTATCAAAGAAAGACAAACTTTACAAAGATGCCATGGAGACAGCCTCACAGTCTGGTGACCGCGAACTTGCGGAGGAATTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCGTCATGCCTCTTCGTCTGTTACGATTTGATTAGGCCGGATACTGCCCTTGAACTTGCTTGGATGAACAACATGATAGACTTTGCCTTCCCATATCTGTTGCAGTTCATCCGTGAATACACCGGTAAAGTCGATGAATTGGTGAAGGACAGAATTGAAGCCCAGAATGAGGTCAAGGAGAAGGAGAAGGAAGAGAAAGAAGTTATAGCACAGCAGAACATGTATGCACAGTTGTTGCCTCTTGCTTTACCTGCTCCACCAATGCCTGGTATGGGAGGAGGGTATGGTCCTCCTCCTCCAATGGGAGGAATGGGGATGCCTCCGATGCCACCTTATGGCATGCCACCAATGGGCAGTAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1700

Amino Acids

192.74

Weight (kDa)

5.27

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 2.9e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 9.3e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 433 3.2e-24 Clathrin-H-link
Clathrin PF00637 556 - 691 7.9e-25 Region in Clathrin and VPS
Clathrin PF00637 700 - 840 7.4e-27 Region in Clathrin and VPS
Clathrin PF00637 849 - 983 1.6e-32 Region in Clathrin and VPS
Clathrin PF00637 992 - 1130 4.1e-32 Region in Clathrin and VPS
Clathrin PF00637 1143 - 1280 1.1e-30 Region in Clathrin and VPS
Clathrin PF00637 1287 - 1431 1e-27 Region in Clathrin and VPS
Clathrin PF00637 1437 - 1578 3.7e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4254
Acc36I ACCTGC 4 cut(s) 1546, 2080, 3205, 4996
Acc65I GGTACC 1 cut(s) 2006
AccB1I GGYRCC 3 cut(s) 743, 2006, 4368
AccB7I CCANNNNNTGG 3 cut(s) 3496, 5003, 5072
AccI GTMKAC 1 cut(s) 891
AccII CGCG 2 cut(s) 4566, 4680
AclI AACGTT 1 cut(s) 80
AcsI RAATTY 8 cut(s) 285, 1209, 1238, 2293, 2933, 3329, 3849, 3902
AcuI CTGAAG 6 cut(s) 1685, 2060, 2535, 3076, 3492, 4209
AcvI CACGTG 1 cut(s) 4340
AflIII ACRYGT 6 cut(s) 88, 727, 1951, 4337, 4339, 4956
AgeI ACCGGT 1 cut(s) 4859
AhlI ACTAGT 1 cut(s) 1990
AjiI CACGTC 3 cut(s) 1346, 2662, 2807
AjuI GAANNNNNNNTTGG 6 cut(s) 1361, 1393, 1835, 1867, 3942, 3974
AloI GAACNNNNNNTCC 2 cut(s) 1758, 1790
Alw21I GWGCWC 6 cut(s) 541, 2661, 3558, 3976, 4044, 4266
Alw26I GTCTC 6 cut(s) 843, 896, 3656, 3863, 4423, 4649
Alw44I GTGCAC 3 cut(s) 2657, 3972, 4262
Ama87I CYCGRG 1 cut(s) 1793
AoxI GGCC 6 cut(s) 36, 191, 548, 1003, 3711, 4772
ApaLI GTGCAC 3 cut(s) 2657, 3972, 4262
ApoI RAATTY 8 cut(s) 285, 1209, 1238, 2293, 2933, 3329, 3849, 3902
ArsI GACNNNNNNTTYG 4 cut(s) 3019, 3051, 3841, 3873
AseI ATTAAT 5 cut(s) 63, 1026, 3050, 3171, 4296
AsiGI ACCGGT 1 cut(s) 4859
Asp718I GGTACC 1 cut(s) 2006
AspLEI GCGC 4 cut(s) 136, 600, 619, 4566
AspS9I GGNCC 6 cut(s) 274, 1003, 1789, 3194, 3711, 5024
AsuC2I CCSGG 1 cut(s) 3389
AsuHPI GGTGA 9 cut(s) 16, 190, 431, 2392, 2470, 3511, 3656, 4685, 4891
AvaI CYCGRG 1 cut(s) 1793
AvaII GGWCC 4 cut(s) 274, 1789, 3194, 5024
BaeGI GKGCMC 6 cut(s) 748, 2513, 2661, 3976, 4266, 4371
BamHI GGATCC 1 cut(s) 2209
BanI GGYRCC 3 cut(s) 743, 2006, 4368
BanII GRGCYC 3 cut(s) 2571, 2993, 3558
BauI CACGAG 2 cut(s) 2745, 4544
BbrPI CACGTG 1 cut(s) 4340
BbsI GAAGAC 4 cut(s) 1445, 2947, 3533, 4470
Bbv12I GWGCWC 6 cut(s) 541, 2661, 3558, 3976, 4044, 4266
BbvCI CCTCAGC 1 cut(s) 3828
BccI CCATC 6 cut(s) 769, 2884, 3143, 3185, 3508, 4178
BceAI ACGGC 1 cut(s) 1067
BcgI CGANNNNNNTGC 2 cut(s) 3179, 3213
BciVI GTATCC 2 cut(s) 1219, 4771
BclI TGATCA 3 cut(s) 2110, 2125, 4510
BcnI CCSGG 1 cut(s) 3389
BcoDI GTCTC 6 cut(s) 843, 896, 3656, 3863, 4423, 4649
BcuI ACTAGT 1 cut(s) 1990
BfaI CTAG 8 cut(s) 909, 1424, 1991, 2283, 2498, 3179, 4014, 4379
BfmI CTRYAG 4 cut(s) 393, 1778, 3198, 3324
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 4 cut(s) 1546, 2080, 3205, 4996
BfuI GTATCC 2 cut(s) 1219, 4771
BglI GCCNNNNNGGC 2 cut(s) 3140, 3719
BglII AGATCT 5 cut(s) 784, 1429, 1690, 2395, 4288
BlpI GCTNAGC 2 cut(s) 2337, 3841
BmcAI AGTACT 1 cut(s) 2105
Bme18I GGWCC 4 cut(s) 274, 1789, 3194, 5024
BmeT110I CYCGRG 1 cut(s) 1793
BmgBI CACGTC 3 cut(s) 1346, 2662, 2807
BmgT120I GGNCC 6 cut(s) 274, 1003, 1789, 3194, 3711, 5024
BmiI GGNNCC 7 cut(s) 648, 745, 1004, 2008, 2211, 2568, 4370
BmrI ACTGGG 4 cut(s) 631, 815, 1876, 3718
BmuI ACTGGG 4 cut(s) 631, 815, 1876, 3718
BoxI GACNNNNGTC 1 cut(s) 1787
BpiI GAAGAC 4 cut(s) 1445, 2947, 3533, 4470
BplI GAGNNNNNCTC 4 cut(s) 1299, 1331, 4646, 4678
BpmI CTGGAG 1 cut(s) 783
Bpu10I CCTNAGC 3 cut(s) 333, 2097, 3828
Bpu1102I GCTNAGC 2 cut(s) 2337, 3841
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 3389
Bsa29I ATCGAT 1 cut(s) 2952
BsaAI YACGTR 1 cut(s) 4340
BsaBI GATNNNNATC 2 cut(s) 1452, 2898
BsaI GGTCTC 2 cut(s) 3656, 3863
BsaJI CCNNGG 7 cut(s) 259, 650, 752, 2182, 3751, 4194, 4650
BsaWI WCCGGW 2 cut(s) 1222, 4859
BsaXI ACNNNNNCTCC 2 cut(s) 1419, 1449
Bse118I RCCGGY 1 cut(s) 4859
Bse3DI GCAATG 3 cut(s) 2552, 2819, 4611
Bse8I GATNNNNATC 2 cut(s) 1452, 2898
BseCI ATCGAT 1 cut(s) 2952
BseDI CCNNGG 7 cut(s) 259, 650, 752, 2182, 3751, 4194, 4650
BseJI GATNNNNATC 2 cut(s) 1452, 2898
BseMI GCAATG 3 cut(s) 2552, 2819, 4611
BseRI GAGGAG 6 cut(s) 202, 3845, 3905, 5019, 5022, 5028
BseSI GKGCMC 6 cut(s) 748, 2513, 2661, 3976, 4266, 4371
BseYI CCCAGC 4 cut(s) 747, 874, 1259, 4364
BsgI GTGCAG 3 cut(s) 195, 933, 2108
Bsh1236I CGCG 2 cut(s) 4566, 4680
Bsh1285I CGRYCG 1 cut(s) 2377
BshFI GGCC 6 cut(s) 38, 193, 550, 1005, 3713, 4774
BshNI GGYRCC 3 cut(s) 743, 2006, 4368
BshTI ACCGGT 1 cut(s) 4859
BshVI ATCGAT 1 cut(s) 2952
BsiEI CGRYCG 1 cut(s) 2377
BsiHKAI GWGCWC 6 cut(s) 541, 2661, 3558, 3976, 4044, 4266
BsiHKCI CYCGRG 1 cut(s) 1793
BsiSI CCGG 4 cut(s) 1223, 3388, 4775, 4860
BslFI GGGAC 5 cut(s) 1183, 1306, 1630, 2502, 3408
BsmAI GTCTC 6 cut(s) 843, 896, 3656, 3863, 4423, 4649
BsmFI GGGAC 5 cut(s) 1183, 1306, 1630, 2502, 3408
BsmI GAATGC 3 cut(s) 1415, 3335, 4736
BsnI GGCC 6 cut(s) 38, 193, 550, 1005, 3713, 4774
Bso31I GGTCTC 2 cut(s) 3656, 3863
BsoBI CYCGRG 1 cut(s) 1793
Bsp1407I TGTACA 2 cut(s) 2412, 3871
Bsp1720I GCTNAGC 2 cut(s) 2337, 3841
Bsp19I CCATGG 1 cut(s) 4650
BspANI GGCC 6 cut(s) 38, 193, 550, 1005, 3713, 4774
BspDI ATCGAT 1 cut(s) 2952
BspFNI CGCG 2 cut(s) 4566, 4680
BspHI TCATGA 2 cut(s) 3024, 4173
BspLI GGNNCC 7 cut(s) 648, 745, 1004, 2008, 2211, 2568, 4370
BspMAI CTGCAG 2 cut(s) 1782, 3202
BspMI ACCTGC 4 cut(s) 1546, 2080, 3205, 4996
BspT107I GGYRCC 3 cut(s) 743, 2006, 4368
BspTNI GGTCTC 2 cut(s) 3656, 3863
BsrDI GCAATG 3 cut(s) 2552, 2819, 4611
BsrFI RCCGGY 1 cut(s) 4859
BsrGI TGTACA 2 cut(s) 2412, 3871
BssAI RCCGGY 1 cut(s) 4859
BssECI CCNNGG 7 cut(s) 259, 650, 752, 2182, 3751, 4194, 4650
BssNAI GTATAC 1 cut(s) 892
BssSI CACGAG 2 cut(s) 2745, 4544
BssT1I CCWWGG 2 cut(s) 3751, 4650
Bst1107I GTATAC 1 cut(s) 892
Bst2BI CACGAG 2 cut(s) 2745, 4544
Bst6I CTCTTC 7 cut(s) 584, 2585, 3342, 3594, 4452, 4754, 4923
BstAPI GCANNNNNTGC 3 cut(s) 632, 1511, 3118
BstAUI TGTACA 2 cut(s) 2412, 3871
BstBAI YACGTR 1 cut(s) 4340
BstC8I GCNNGC 7 cut(s) 466, 621, 1080, 1914, 3588, 4317, 5078
BstDSI CCRYGG 1 cut(s) 4650
BstEII GGTNACC 1 cut(s) 4673
BstENI CCTNNNNNAGG 2 cut(s) 2576, 3671
BstFNI CGCG 2 cut(s) 4566, 4680
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 4 cut(s) 136, 600, 619, 4566
BstMAI GTCTC 6 cut(s) 843, 896, 3656, 3863, 4423, 4649
BstMCI CGRYCG 1 cut(s) 2377
BstNSI RCATGY 8 cut(s) 92, 623, 731, 1772, 1973, 4207, 4960, 5080
BstPAI GACNNNNGTC 1 cut(s) 1787
BstPI GGTNACC 1 cut(s) 4673
BstSFI CTRYAG 4 cut(s) 393, 1778, 3198, 3324
BstSLI GKGCMC 6 cut(s) 748, 2513, 2661, 3976, 4266, 4371
BstUI CGCG 2 cut(s) 4566, 4680
BstV2I GAAGAC 4 cut(s) 1445, 2947, 3533, 4470
BstXI CCANNNNNNTGG 1 cut(s) 3160
BstZ17I GTATAC 1 cut(s) 892
Bsu15I ATCGAT 1 cut(s) 2952
BsuI GTATCC 2 cut(s) 1219, 4771
BsuRI GGCC 6 cut(s) 38, 193, 550, 1005, 3713, 4774
BsuTUI ATCGAT 1 cut(s) 2952
BtgI CCRYGG 1 cut(s) 4650
BtgZI GCGATG 1 cut(s) 2832
BtrI CACGTC 3 cut(s) 1346, 2662, 2807
BtsI GCAGTG 1 cut(s) 1420
BtsIMutI CAGTG 8 cut(s) 978, 1060, 1250, 1420, 2568, 3100, 3556, 3904
BveI ACCTGC 4 cut(s) 1546, 2080, 3205, 4996
Cac8I GCNNGC 7 cut(s) 466, 621, 1080, 1914, 3588, 4317, 5078
CciI TCATGA 2 cut(s) 3024, 4173
CfoI GCGC 4 cut(s) 136, 600, 619, 4566
Cfr10I RCCGGY 1 cut(s) 4859
Cfr13I GGNCC 6 cut(s) 274, 1003, 1789, 3194, 3711, 5024
ClaI ATCGAT 1 cut(s) 2952
CseI GACGC 1 cut(s) 4728
CspAI ACCGGT 1 cut(s) 4859
DraI TTTAAA 1 cut(s) 4068
Eam1104I CTCTTC 7 cut(s) 584, 2585, 3342, 3594, 4452, 4754, 4923
EarI CTCTTC 7 cut(s) 584, 2585, 3342, 3594, 4452, 4754, 4923
EciI GGCGGA 1 cut(s) 3446
Ecl136II GAGCTC 1 cut(s) 3556
Eco130I CCWWGG 2 cut(s) 3751, 4650
Eco147I AGGCCT 2 cut(s) 38, 193
Eco24I GRGCYC 3 cut(s) 2571, 2993, 3558
Eco31I GGTCTC 2 cut(s) 3656, 3863
Eco47I GGWCC 4 cut(s) 274, 1789, 3194, 5024
Eco53kI GAGCTC 1 cut(s) 3556
Eco57I CTGAAG 6 cut(s) 1685, 2060, 2535, 3076, 3492, 4209
Eco72I CACGTG 1 cut(s) 4340
Eco88I CYCGRG 1 cut(s) 1793
Eco91I GGTNACC 1 cut(s) 4673
EcoICRI GAGCTC 1 cut(s) 3556
EcoNI CCTNNNNNAGG 2 cut(s) 2576, 3671
EcoO109I RGGNCCY 1 cut(s) 1789
EcoO65I GGTNACC 1 cut(s) 4673
EcoRI GAATTC 2 cut(s) 1209, 3902
EcoT14I CCWWGG 2 cut(s) 3751, 4650
EcoT22I ATGCAT 2 cut(s) 2135, 3568
EcoT38I GRGCYC 3 cut(s) 2571, 2993, 3558
ErhI CCWWGG 2 cut(s) 3751, 4650
FaqI GGGAC 5 cut(s) 1183, 1306, 1630, 2502, 3408
FauI CCCGC 3 cut(s) 993, 1209, 1620
FauNDI CATATG 3 cut(s) 116, 2161, 3568
FbaI TGATCA 3 cut(s) 2110, 2125, 4510
FblI GTMKAC 1 cut(s) 891
FriOI GRGCYC 3 cut(s) 2571, 2993, 3558
FspBI CTAG 8 cut(s) 909, 1424, 1991, 2283, 2498, 3179, 4014, 4379
GlaI GCGC 4 cut(s) 135, 599, 618, 4565
GsaI CCCAGC 4 cut(s) 751, 878, 1263, 4368
GsuI CTGGAG 1 cut(s) 783
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 6 cut(s) 38, 193, 550, 1005, 3713, 4774
HapII CCGG 4 cut(s) 1223, 3388, 4775, 4860
HgaI GACGC 1 cut(s) 4728
HhaI GCGC 4 cut(s) 136, 600, 619, 4566
Hin6I GCGC 4 cut(s) 134, 598, 617, 4564
HinP1I GCGC 4 cut(s) 134, 598, 617, 4564
HindIII AAGCTT 1 cut(s) 963
HpaII CCGG 4 cut(s) 1223, 3388, 4775, 4860
HphI GGTGA 9 cut(s) 16, 190, 431, 2392, 2470, 3511, 3656, 4685, 4891
Hpy99I CGWCG 1 cut(s) 2808
HspAI GCGC 4 cut(s) 134, 598, 617, 4564
KpnI GGTACC 1 cut(s) 2010
Ksp22I TGATCA 3 cut(s) 2110, 2125, 4510
MaeI CTAG 8 cut(s) 909, 1424, 1991, 2283, 2498, 3179, 4014, 4379
MfeI CAATTG 4 cut(s) 411, 554, 1038, 4488
MlyI GAGTC 8 cut(s) 110, 200, 431, 1205, 1288, 1337, 3373, 3425
MmeI TCCRAC 3 cut(s) 2010, 3352, 3729
Mph1103I ATGCAT 2 cut(s) 2135, 3568
MslI CAYNNNNRTG 4 cut(s) 299, 833, 3611, 4338
MspA1I CMGCKG 3 cut(s) 1259, 2243, 4424
MspI CCGG 4 cut(s) 1223, 3388, 4775, 4860
MunI CAATTG 4 cut(s) 411, 554, 1038, 4488
Mva1269I GAATGC 3 cut(s) 1415, 3335, 4736
MvnI CGCG 2 cut(s) 4566, 4680
NciI CCSGG 1 cut(s) 3389
NcoI CCATGG 1 cut(s) 4650
NdeI CATATG 3 cut(s) 116, 2161, 3568
NlaIV GGNNCC 7 cut(s) 648, 745, 1004, 2008, 2211, 2568, 4370
NmeAIII GCCGAG 1 cut(s) 1540
NmuCI GTSAC 5 cut(s) 382, 1341, 1986, 3724, 4673
NsiI ATGCAT 2 cut(s) 2135, 3568
NspI RCATGY 8 cut(s) 92, 623, 731, 1772, 1973, 4207, 4960, 5080
PaeI GCATGC 2 cut(s) 623, 5080
PaeR7I CTCGAG 1 cut(s) 1793
PagI TCATGA 2 cut(s) 3024, 4173
PceI AGGCCT 2 cut(s) 38, 193
PciI ACATGT 3 cut(s) 88, 727, 4956
PcsI WCGNNNNNNNCGW 1 cut(s) 4758
PctI GAATGC 3 cut(s) 1415, 3335, 4736
PflMI CCANNNNNTGG 3 cut(s) 3496, 5003, 5072
PfoI TCCNGGA 1 cut(s) 3387
PinAI ACCGGT 1 cut(s) 4859
PleI GAGTC 8 cut(s) 109, 200, 430, 1204, 1288, 1336, 3372, 3424
PmaCI CACGTG 1 cut(s) 4340
PmlI CACGTG 1 cut(s) 4340
PpsI GAGTC 8 cut(s) 109, 200, 430, 1204, 1288, 1336, 3372, 3424
Ppu21I YACGTR 1 cut(s) 4340
PpuMI RGGWCCY 1 cut(s) 1789
PscI ACATGT 3 cut(s) 88, 727, 4956
PshAI GACNNNNGTC 1 cut(s) 1787
PshBI ATTAAT 5 cut(s) 63, 1026, 3050, 3171, 4296
PsiI TTATAA 1 cut(s) 4254
Psp124BI GAGCTC 1 cut(s) 3558
Psp1406I AACGTT 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 1789
PspCI CACGTG 1 cut(s) 4340
PspEI GGTNACC 1 cut(s) 4673
PspFI CCCAGC 4 cut(s) 747, 874, 1259, 4364
PspN4I GGNNCC 7 cut(s) 648, 745, 1004, 2008, 2211, 2568, 4370
PspPI GGNCC 6 cut(s) 274, 1003, 1789, 3194, 3711, 5024
PspPPI RGGWCCY 1 cut(s) 1789
PstI CTGCAG 2 cut(s) 1782, 3202
PvuII CAGCTG 3 cut(s) 1259, 2243, 4424
RseI CAYNNNNRTG 4 cut(s) 299, 833, 3611, 4338
SacI GAGCTC 1 cut(s) 3558
Sau96I GGNCC 6 cut(s) 274, 1003, 1789, 3194, 3711, 5024
ScaI AGTACT 1 cut(s) 2105
SchI GAGTC 8 cut(s) 110, 200, 431, 1205, 1288, 1337, 3373, 3425
SfcI CTRYAG 4 cut(s) 393, 1778, 3198, 3324
Sfr274I CTCGAG 1 cut(s) 1793
SinI GGWCC 4 cut(s) 274, 1789, 3194, 5024
SlaI CTCGAG 1 cut(s) 1793
SmiMI CAYNNNNRTG 4 cut(s) 299, 833, 3611, 4338
SmlI CTYRAG 2 cut(s) 252, 1793
SmoI CTYRAG 2 cut(s) 252, 1793
SpeI ACTAGT 1 cut(s) 1990
SphI GCATGC 2 cut(s) 623, 5080
SseBI AGGCCT 2 cut(s) 38, 193
SspI AATATT 2 cut(s) 2762, 4072
SspMI CTAG 8 cut(s) 909, 1424, 1991, 2283, 2498, 3179, 4014, 4379
SstI GAGCTC 1 cut(s) 3558
StuI AGGCCT 2 cut(s) 38, 193
StyI CCWWGG 2 cut(s) 3751, 4650
TaqI TCGA 8 cut(s) 1794, 2599, 2803, 2952, 3189, 3463, 4087, 4869
TatI WGTACW 9 cut(s) 111, 639, 846, 1167, 2103, 2172, 2229, 2412, 3871
TauI GCSGC 7 cut(s) 8, 11, 139, 553, 626, 1984, 3011
TscAI CASTG 8 cut(s) 978, 1060, 1250, 1420, 2568, 3100, 3556, 3904
TseFI GTSAC 5 cut(s) 382, 1341, 1986, 3724, 4673
Tsp45I GTSAC 5 cut(s) 382, 1341, 1986, 3724, 4673
TspGWI ACGGA 5 cut(s) 2420, 3138, 3329, 4313, 4838
TspRI CASTG 8 cut(s) 978, 1060, 1250, 1420, 2568, 3100, 3556, 3904
Van91I CCANNNNNTGG 3 cut(s) 3496, 5003, 5072
VneI GTGCAC 3 cut(s) 2657, 3972, 4262
VpaK11BI GGWCC 4 cut(s) 274, 1789, 3194, 5024
VspI ATTAAT 5 cut(s) 63, 1026, 3050, 3171, 4296
XagI CCTNNNNNAGG 2 cut(s) 2576, 3671
XapI RAATTY 8 cut(s) 285, 1209, 1238, 2293, 2933, 3329, 3849, 3902
XceI RCATGY 8 cut(s) 92, 623, 731, 1772, 1973, 4207, 4960, 5080
XcmI CCANNNNNNNNNTGG 2 cut(s) 1655, 3448
XhoI CTCGAG 1 cut(s) 1793
XmiI GTMKAC 1 cut(s) 891
XspI CTAG 8 cut(s) 909, 1424, 1991, 2283, 2498, 3179, 4014, 4379
ZrmI AGTACT 1 cut(s) 2105
Zsp2I ATGCAT 2 cut(s) 2135, 3568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.