Rw5G042930

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002740
Physical Location & Seq
Forward (+)
75069514 .. 75079573
10060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042930.1

Sequence Viewer

Length: 5085 bp
ATGGCCGCCGCTGCCAATGCCCCCATGACCATGAAAGAGCAGCTCACGCTGCCTAGTATTGGGATTAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGCGTTCGGGAGACCTCGCCGCAGAATAGCATCGTGATCGTTGACATGAACATGCCAATGCAGCCGTTGCGACGGCCGATTACTGCTGATTCGGCTTTGATGAATCCTAATTCGAGGATTCTGGCTCTTAAAGCTCAAGTGCAAGGAACTACTCAGGATCATTTACAGATATTTAACATTGAACTGAAAACGAAGATGAAATCACATCAGATGCCTGAGCAGATGCTGGGGATGGTCACACAGACCTCAGTATACCACTGGTTAATTGACGGGGAGTCTGAGCCAACAAAGGTGTTTGAGCGTACAGCTAATTTAGCAAATAATCAAATTATCAACTACCGTTGTGATCCCTCTGAAAAGTGGTTGGTCTTAATTGGAATTGCTCCTGGTTCTCCTGAGAGGCCACAACTGGTTAAAGGAAACATGCAGCTTTTCTCTGTGGAGCAGCAACGTAGTCAGGCTCTTGAAGCACATGCTGCATCATTTGCCCCATTTAAAGTTGCAGGGAACGAGAACCCTTCAATTCTTATCTGCTTTGCTGCAAAGTCCTCCAACGCTGGACAAGTTACATCAAAGTTGCATGTTATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATATACTAAGAAGCAAGCAGATCTATTCTTTCCTCCGGATTTTGCTGATGACTTTCCTGTGTCAATGCAGATATCTGAAAAGTATGGGTTGATATATGTTATTACAAAGCTTGGACTACTGTTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCGGATCCTATATTTTTGACAGCTGAAGCTTCATCTGTTGGAGGTTTTTATGCAGTCAACAGGCGTGGGCAGGTGTTACTTGCCACTGTAAATGAACAAACAATTGTACCTTTTGTTAGTGGTCAGTTAAACAATTTGGGACTCGCAGTTAGTCTTGCCAGAAGAGGAAATCTTCCTGGTGCTGAAGACTTGGTTGTCCAGCGGTTCCAAGAATTGTTTTCTCAGACAAAATATAAGGAGGCTGCAGAGCTTGCTGCAGATTCTCCAATGGGAGTACTCCGTACACCTGAAACTGTTGCTAAATTTCAGGGTGTTCCTGTGCAAGCTGGGCAAACACCACCATTGTTGCAGTACTTTGGAACTTTGTTAACTAAAGGGAAACTCAACGCTTTTGAATCATTGGAACTGTCACGTTTGGTTGTAAATCAAAACAAAAAGAATCTTTTGGAGAATTGGCTAGCGGAGGACAAGCTTGAATGTAGCGAAGAGCTAGGAGATCTTGTGAAGACAGTGGATAATGACCTTGCTTTGAAAATATACGTCAAAGCCAGGGCAACTCCAAAAGTTGTTGCAGCCTTTGCTGAGAGAAGGGAATTTGACAAGATTTTAATATACTCAAAACAGGTTGGATATACACCTGATTATATGTTCCTTCTGCAAACAATTTTGCGGACAGATCCCCAGGGGGCGGTCAATTTTGCACTAATGATGTCTCAAATGGAAGGAGGTTGCCCTATTGACTACAATACCATTACTGATCTGTTCCTTCAGAGGAATTTGATTCGTGAGGCAACAGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCTGAGCATGCCCACCTTCAGACAAAGGTTTTGGAAATCAATCTAGTGACTTTTCCCAATGTTGCGGATGCTATATTGGCCAACGGTATGTTCAGTCATTATGACCGCCCCCGCATTGGTCAGCTCTGTGAAAAAGCTGGTTTGTATTTGCGTGCCTTACAGCATTATACCGAGTTACCTGATATTAAAAGAGTCATTGTGAACACTCATGCAATTGACCCTCAGGCTCTTGTTGAGTTCTTCGGGACTCTTTCCATAGAATGGGCTCTTGAGTGCATGAAAGATCTTCTACTGGTCAATCTTAGGGGCAATCTTCAGATTATTGTGCAGACTGCCAAAGAGTATTCCGAGCAACTGGGTGTAGATAAATGTATAAAGTTGTTTGAGCAGTTCAAGTCCTATGAGGGGTTGTACTTTTTCTTAGGCTCATACTTGAGCTCCAGTGAGGATCCCGATATCCACTTTAAGTATATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGAGTGACTCGTGAATCTAATTTTTATGATGCTGAGAAGGCTAAGAACTTTCTGATGGAAGCAAAGCTTCCAGATGCGCGTCCCCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCTGATCTCACTCATTACCTATATACGAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTTGGGCAGCTTTTGGATGATGAATGTCCTGAAGATTTCATCAAAGGACTTATTCTATCAGTTCGTTCTCTTCTACCAGTCGAGCCCCTTGTTGATGAATGTGAAAAGAGGAATCGTCTTCGCCTGCTCACTCAGTTCTTGGAGCATCTTGTGAGTGAAGGAAGCCAAGATGTACATGTTCATAATGCTCTTGGTAAAATCATCATAGATAGTGGCAACAATCCAGAGCATTTTCTCACAACTAACCCATATTATGATTCTCGCGTTGTGGGTAAATATTGTGAAAAAAGAGATCCCACCCTTGCTGTTGTTGCTTACCGTAGAGGGCAATGTGATGATGAACTTGTTAATGTAACAAATAAGAATTCATTATTCAAACTCCAGGCCAGATATGTTGTGGAAAGAATGGATGATGACCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAGTATAGAAGGCAGCTAATTGATCAAGTTGTCTCTACTGCTTTGCCTGAAAGCAAGAGCCCTGAGCAGGTTTCAGCAACTGTTAAAGCGTTTATGACTGCAGACCTTCCACATGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTCCAAAATTCAGCTTTTAGTGGGAACTTCAATCTACAGAATCTGTTGATCTTGACTGCAATTAAGGCTGATCCATCCAGAGTCATGGATTATATCAATAGATTAGACAACTTTGATGGACCTGCGGTTGGCGTAATGGCTGTAGAAGCCCAACTCTATGAGGAAGCATTTGCAATCTTTAAGAAGTTCAATTTGAATGTTGATGCTGTCAATGTTCTGTTGGATAACATTCAGAGCATTGACCGTGCCGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTTGCCAAGGCCCAACTACGAGAGGGGCTTGTGAGTGATGCTATTGAGTCATTCATTCATGCTGATGATGCCACCCAGTTTTTGGATGTCATACGTGCTGCTCAGGATGCAGATGTGTATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGGTGAAAGAGCCCAAGGTAGACAGTGAACTCATTTATGCATATGCTAAGATTGATCGACTTGGTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCTAATCTCCCAAATGTTGGGGATCGCCTGTATGATGAAGAACTATATGAAGCAGCCAAGATCATATTTGCATTTATTTCTAATTGGGCCAAGCTGGCGTGTACTCTAGTGAAGCTCAAGCAGTTTCAAGGTGCTGTTGATGCCGCCCGGAAAGCTAACAGCTCAAAAACTTGGAAGGAAGTTTGCTTTGCATGTGTTGATGCTGAGGAATTCCGGTTGGCTCAAATTTGTGGACTCAACATCATCATCCAGGTGGATGATTTGGAAGAGGTCAGTGAGTTTTACCAGAACAGGGGTTGCTTTAATGAGCTCATTAATCTTATGGAGAGTGGACTAGGATTGGAACGTGCACATATGGGCATTTTTACTGAATTAGGAGTTCTATATGCAAGATACCGTCCTGAGAAGCTTATGGAGCACATTAAACTGTTTTCTACTCGTCTCAACATTCCCAAACTTATACGAGCTTGTGATGAACAGCAGCACTGGAAGGAATTAACATACTTATATATACAATACGATGAATTTGATAATGCTGCAACCACCATAATGAACCACTCCCCAGAAGCATGGGAACACATGCAATTCAAGGATGTGATAGTCAAAGTTGCAAGTGTTGAGCTTTACTATAGGGCTGTGCACTTTTACTTAGAAGAACATCCTGATATTATCAATGATGTTCTAAATGTGCTTGCGCTTCGTGTGGATCATACCCGAGTGGTGGATATAATGCGAAAGGCTGGTCACATCCGTCTGGTAAAGCCATACATGGTTGCAGTCCAGAGCAACAATGTAACAGCTGTAAATGAAGCTCTAAATGAAATATATATTGAGGAGGAAGACTATGACAGACTTCGTGAATCTATAGATTTGCATGATAGCTTTGACCAGATAGGTCTTGCACAAAAGCTTGAGAAACATGAGCTTCTTGAGATGAGACGTGTTGCTGCCTACATTTATAAGAAAGCTGGAAGATGGAAGCAATCCATTGCATTGTCAAAGAAAGACAAACATTACAAAGACGCCATGGAGACATGCTCGCAGTCTGGTGATCATGAACTTTCAGAGGAATTGCTTGTCTATTTCATCGAGCAGGGAAAGAAAGAATGTTTCGCATCGTGTCTCTTTGTTTGTTTTGATTTAATCCGGCCAGATGTTGCTCTTGAGCTGGCATGGATGAACAATATAATTGATTTTGCATTCCCATATATCCTGCAGTTTATTCGTGAGTACACAGGCAAGGTTGATGAACTTGTCAAAGACAAAATTGAGACTCAGAAAGAGGTGAAAGCCAAAGAGCAAGAGGAGAAGGAATTGGTTGCTAAGCAGAACATGTATGCTCAGTTGCTGCCTGCTTTGCCTGCACCTCCGGGAATGGGTGGAGGTTACGCTCCACCTCCAATGCCACCAATGCCACAGATGGGTGGGATGGGGATGCCTCCAATGCCACCTTTCGGAATGCCACAAATGGGCCCTTCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1694

Amino Acids

191.89

Weight (kDa)

5.24

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 21 - 57 2.5e-06 Clathrin propeller repeat
Clathrin-link PF09268 335 - 357 6.2e-08 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 360 - 425 1.2e-26 Clathrin-H-link
Clathrin PF00637 548 - 683 6.4e-25 Region in Clathrin and VPS
Clathrin PF00637 692 - 832 7.1e-27 Region in Clathrin and VPS
Clathrin PF00637 841 - 975 2.3e-32 Region in Clathrin and VPS
Clathrin PF00637 984 - 1122 3e-32 Region in Clathrin and VPS
Clathrin PF00637 1137 - 1272 2.1e-29 Region in Clathrin and VPS
Clathrin PF00637 1281 - 1422 7.6e-26 Region in Clathrin and VPS
Clathrin PF00637 1429 - 1570 8.2e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4560
AarI CACCTGC 1 cut(s) 970
AasI GACNNNNNNGTC 1 cut(s) 1103
Acc36I ACCTGC 3 cut(s) 970, 2965, 3181
Acc65I GGTACC 1 cut(s) 3480
AccB1I GGYRCC 2 cut(s) 716, 3480
AccB7I CCANNNNNTGG 3 cut(s) 1998, 3427, 3617
AccI GTMKAC 2 cut(s) 372, 3522
AccII CGCG 2 cut(s) 2329, 2724
AccIII TCCGGA 2 cut(s) 772, 910
AcoI YGGCCR 4 cut(s) 3, 194, 1815, 4748
AcsI RAATTY 8 cut(s) 1211, 1502, 1684, 2824, 3058, 3809, 3825, 4124
AcuI CTGAAG 7 cut(s) 954, 1113, 1661, 1739, 1745, 2036, 2511
AcyI GRCGYC 1 cut(s) 4623
AflIII ACRYGT 3 cut(s) 2635, 4540, 4932
AhdI GACNNNNNGTC 1 cut(s) 394
AjiI CACGTC 1 cut(s) 4541
AjnI CCWGG 9 cut(s) 505, 724, 1084, 1457, 1590, 2440, 2841, 3342, 3849
AjuI GAANNNNNNNTTGG 2 cut(s) 1811, 1843
Alw21I GWGCWC 6 cut(s) 2177, 3054, 3912, 3952, 4020, 4242
Alw26I GTCTC 9 cut(s) 125, 869, 1626, 2944, 4046, 4531, 4625, 4727, 4865
Alw44I GTGCAC 2 cut(s) 3948, 4238
AlwNI CAGNNNCTG 5 cut(s) 346, 884, 2987, 3094, 4948
Ama87I CYCGRG 1 cut(s) 4314
Aor13HI TCCGGA 2 cut(s) 772, 910
AoxI GGCC 9 cut(s) 3, 194, 521, 1815, 2844, 3354, 3687, 4748, 5071
ApaI GGGCCC 1 cut(s) 5075
ApaLI GTGCAC 2 cut(s) 3948, 4238
ApoI RAATTY 8 cut(s) 1211, 1502, 1684, 2824, 3058, 3809, 3825, 4124
ArsI GACNNNNNNTTYG 4 cut(s) 1750, 1782, 4327, 4359
AseI ATTAAT 3 cut(s) 66, 2340, 3915
Asp718I GGTACC 1 cut(s) 3480
AspLEI GCGC 2 cut(s) 2329, 4297
AspS9I GGNCC 6 cut(s) 2890, 3170, 3355, 3687, 5071, 5072
AsuC2I CCSGG 3 cut(s) 3317, 3748, 4971
AsuHPI GGTGA 4 cut(s) 3517, 3578, 4661, 4897
AsuNHI GCTAGC 1 cut(s) 1366
AvaI CYCGRG 1 cut(s) 4314
AvaII GGWCC 2 cut(s) 2890, 3170
AxyI CCTNAGG 1 cut(s) 1959
BaeGI GKGCMC 4 cut(s) 721, 3952, 4242, 5075
BaeI ACNNNNGTAYC 2 cut(s) 999, 1032
BalI TGGCCA 1 cut(s) 1817
BamHI GGATCC 2 cut(s) 913, 2185
BanI GGYRCC 2 cut(s) 716, 3480
BanII GRGCYC 7 cut(s) 2005, 2177, 2547, 2969, 3516, 3912, 5075
BarI GAAGNNNNNNTAC 2 cut(s) 3468, 3500
BauI CACGAG 1 cut(s) 2259
BbsI GAAGAC 4 cut(s) 1101, 1421, 2570, 4446
Bbv12I GWGCWC 6 cut(s) 2177, 3054, 3912, 3952, 4020, 4242
BbvCI CCTCAGC 1 cut(s) 3804
BccI CCATC 9 cut(s) 346, 742, 2299, 3133, 3161, 3484, 4569, 5014, 5023
BceAI ACGGC 3 cut(s) 169, 209, 3284
BcgI CGANNNNNNTGC 6 cut(s) 139, 173, 2383, 2417, 4805, 4839
BciT130I CCWGG 9 cut(s) 507, 726, 1086, 1459, 1592, 2442, 2843, 3344, 3851
BclI TGATCA 2 cut(s) 2929, 4651
BcnI CCSGG 3 cut(s) 3317, 3748, 4971
BcoDI GTCTC 9 cut(s) 125, 869, 1626, 2944, 4046, 4531, 4625, 4727, 4865
BfaI CTAG 7 cut(s) 54, 872, 1367, 1400, 1781, 3707, 3935
BfmI CTRYAG 9 cut(s) 885, 1152, 1164, 3006, 3086, 3192, 4228, 4464, 4814
BfuAI ACCTGC 3 cut(s) 970, 2965, 3181
BglI GCCNNNNNGGC 1 cut(s) 3695
BglII AGATCT 3 cut(s) 757, 1405, 2020
BlpI GCTNAGC 1 cut(s) 4923
BmcAI AGTACT 2 cut(s) 1185, 1262
Bme18I GGWCC 2 cut(s) 2890, 3170
BmeRI GACNNNNNGTC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 4314
BmgBI CACGTC 1 cut(s) 4541
BmgT120I GGNCC 6 cut(s) 2890, 3170, 3355, 3687, 5071, 5072
BmiI GGNNCC 7 cut(s) 718, 915, 1115, 2187, 3341, 3482, 5073
BmrI ACTGGG 2 cut(s) 2102, 3415
BmtI GCTAGC 1 cut(s) 1370
BmuI ACTGGG 2 cut(s) 2102, 3415
BpiI GAAGAC 4 cut(s) 1101, 1421, 2570, 4446
BplI GAGNNNNNCTC 2 cut(s) 4622, 4654
BpmI CTGGAG 2 cut(s) 2161, 2825
Bpu10I CCTNAGC 6 cut(s) 336, 1740, 2886, 2970, 3447, 3804
Bpu1102I GCTNAGC 1 cut(s) 4923
BpuEI CTTGAG 7 cut(s) 240, 2027, 2191, 3701, 4532, 4550, 4784
BpuMI CCSGG 3 cut(s) 3317, 3748, 4971
BsaAI YACGTR 1 cut(s) 3440
BsaBI GATNNNNATC 1 cut(s) 3462
BsaHI GRCGYC 1 cut(s) 4623
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 6 cut(s) 1458, 1590, 1591, 3351, 3516, 4626
BsaWI WCCGGW 3 cut(s) 772, 910, 3813
BsaXI ACNNNNNCTCC 4 cut(s) 1395, 1425, 2159, 2189
Bse1I ACTGG 9 cut(s) 383, 534, 2034, 2097, 2178, 2233, 2537, 3421, 4091
Bse21I CCTNAGG 1 cut(s) 1959
Bse3DI GCAATG 2 cut(s) 2795, 4587
Bse8I GATNNNNATC 1 cut(s) 3462
BseAI TCCGGA 2 cut(s) 772, 910
BseBI CCWGG 9 cut(s) 507, 726, 1086, 1459, 1592, 2442, 2843, 3344, 3851
BseDI CCNNGG 6 cut(s) 1458, 1590, 1591, 3351, 3516, 4626
BseJI GATNNNNATC 1 cut(s) 3462
BseMI GCAATG 2 cut(s) 2795, 4587
BseNI ACTGG 9 cut(s) 383, 534, 2034, 2097, 2178, 2233, 2537, 3421, 4091
BseRI GAGGAG 2 cut(s) 4448, 4919
BseSI GKGCMC 4 cut(s) 721, 3952, 4242, 5075
BseX3I CGGCCG 1 cut(s) 194
BseYI CCCAGC 3 cut(s) 346, 720, 1235
BsgI GTGCAG 2 cut(s) 2084, 4947
Bsh1236I CGCG 2 cut(s) 2329, 2724
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 9 cut(s) 5, 196, 523, 1817, 2846, 3356, 3689, 4750, 5073
BshNI GGYRCC 2 cut(s) 716, 3480
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 6 cut(s) 2177, 3054, 3912, 3952, 4020, 4242
BsiHKCI CYCGRG 1 cut(s) 4314
BsiSI CCGG 7 cut(s) 773, 911, 3316, 3748, 3814, 4747, 4970
BslFI GGGAC 3 cut(s) 1062, 1996, 2316
BsmAI GTCTC 9 cut(s) 125, 869, 1626, 2944, 4046, 4531, 4625, 4727, 4865
BsmBI CGTCTC 2 cut(s) 4046, 4531
BsmFI GGGAC 3 cut(s) 1062, 1996, 2316
BsmI GAATGC 3 cut(s) 3311, 4799, 5064
BsnI GGCC 9 cut(s) 5, 196, 523, 1817, 2846, 3356, 3689, 4750, 5073
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 4314
Bsp120I GGGCCC 1 cut(s) 5071
Bsp13I TCCGGA 2 cut(s) 772, 910
Bsp1407I TGTACA 1 cut(s) 2632
Bsp1720I GCTNAGC 1 cut(s) 4923
Bsp19I CCATGG 1 cut(s) 4626
BspANI GGCC 9 cut(s) 5, 196, 523, 1817, 2846, 3356, 3689, 4750, 5073
BspEI TCCGGA 2 cut(s) 772, 910
BspFNI CGCG 2 cut(s) 2329, 2724
BspHI TCATGA 2 cut(s) 3466, 4654
BspLI GGNNCC 7 cut(s) 718, 915, 1115, 2187, 3341, 3482, 5073
BspMAI CTGCAG 5 cut(s) 889, 1156, 1168, 3010, 4818
BspMI ACCTGC 3 cut(s) 970, 2965, 3181
BspOI GCTAGC 1 cut(s) 1370
BspQI GCTCTTC 1 cut(s) 1389
BspT107I GGYRCC 2 cut(s) 716, 3480
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 2795, 4587
BsrGI TGTACA 1 cut(s) 2632
BsrI ACTGG 9 cut(s) 383, 534, 2034, 2097, 2178, 2233, 2537, 3421, 4091
BssECI CCNNGG 6 cut(s) 1458, 1590, 1591, 3351, 3516, 4626
BssNAI GTATAC 1 cut(s) 373
BssNI GRCGYC 1 cut(s) 4623
BssSI CACGAG 1 cut(s) 2259
BssT1I CCWWGG 3 cut(s) 3351, 3516, 4626
Bst1107I GTATAC 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 2259
Bst2UI CCWGG 9 cut(s) 507, 726, 1086, 1459, 1592, 2442, 2843, 3344, 3851
Bst6I CTCTTC 5 cut(s) 1066, 1389, 2535, 3570, 3861
BstACI GRCGYC 1 cut(s) 4623
BstAPI GCANNNNNTGC 5 cut(s) 187, 596, 605, 1160, 1487
BstAUI TGTACA 1 cut(s) 2632
BstBAI YACGTR 1 cut(s) 3440
BstDSI CCRYGG 1 cut(s) 4626
BstFNI CGCG 2 cut(s) 2329, 2724
BstHHI GCGC 2 cut(s) 2329, 4297
BstMAI GTCTC 9 cut(s) 125, 869, 1626, 2944, 4046, 4531, 4625, 4727, 4865
BstMCI CGRYCG 1 cut(s) 197
BstNI CCWGG 9 cut(s) 507, 726, 1086, 1459, 1592, 2442, 2843, 3344, 3851
BstSFI CTRYAG 9 cut(s) 885, 1152, 1164, 3006, 3086, 3192, 4228, 4464, 4814
BstSLI GKGCMC 4 cut(s) 721, 3952, 4242, 5075
BstUI CGCG 2 cut(s) 2329, 2724
BstV2I GAAGAC 4 cut(s) 1101, 1421, 2570, 4446
BstX2I RGATCY 7 cut(s) 757, 913, 1405, 1585, 2020, 2185, 2752
BstXI CCANNNNNNTGG 2 cut(s) 3136, 4170
BstYI RGATCY 7 cut(s) 757, 913, 1405, 1585, 2020, 2185, 2752
BstZ17I GTATAC 1 cut(s) 373
BstZI CGGCCG 1 cut(s) 194
Bsu36I CCTNAGG 1 cut(s) 1959
BsuRI GGCC 9 cut(s) 5, 196, 523, 1817, 2846, 3356, 3689, 4750, 5073
BtgI CCRYGG 1 cut(s) 4626
BtrI CACGTC 1 cut(s) 4541
BtsIMutI CAGTG 7 cut(s) 376, 993, 1425, 2185, 3532, 3880, 4084
BveI ACCTGC 3 cut(s) 970, 2965, 3181
CaiI CAGNNNCTG 5 cut(s) 346, 884, 2987, 3094, 4948
CciI TCATGA 2 cut(s) 3466, 4654
CfoI GCGC 2 cut(s) 2329, 4297
Cfr13I GGNCC 6 cut(s) 2890, 3170, 3355, 3687, 5071, 5072
CseI GACGC 2 cut(s) 2318, 4631
CspCI CAANNNNNGTGG 6 cut(s) 955, 990, 1236, 1271, 3006, 3041
DraI TTTAAA 1 cut(s) 616
DrdI GACNNNNNNGTC 1 cut(s) 1103
DriI GACNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 1103
EaeI YGGCCR 4 cut(s) 3, 194, 1815, 4748
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 5 cut(s) 1066, 1389, 2535, 3570, 3861
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 5 cut(s) 1066, 1389, 2535, 3570, 3861
Ecl136II GAGCTC 2 cut(s) 2175, 3910
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 3 cut(s) 3351, 3516, 4626
Eco24I GRGCYC 7 cut(s) 2005, 2177, 2547, 2969, 3516, 3912, 5075
Eco31I GGTCTC 1 cut(s) 125
Eco32I GATATC 2 cut(s) 810, 2194
Eco47I GGWCC 2 cut(s) 2890, 3170
Eco52I CGGCCG 1 cut(s) 194
Eco53kI GAGCTC 2 cut(s) 2175, 3910
Eco57I CTGAAG 7 cut(s) 954, 1113, 1661, 1739, 1745, 2036, 2511
Eco81I CCTNAGG 1 cut(s) 1959
Eco88I CYCGRG 1 cut(s) 4314
EcoICRI GAGCTC 2 cut(s) 2175, 3910
EcoO109I RGGNCCY 2 cut(s) 2890, 5072
EcoRI GAATTC 2 cut(s) 2824, 3809
EcoRII CCWGG 9 cut(s) 505, 724, 1084, 1457, 1590, 2440, 2841, 3342, 3849
EcoRV GATATC 2 cut(s) 810, 2194
EcoT14I CCWWGG 3 cut(s) 3351, 3516, 4626
EcoT22I ATGCAT 1 cut(s) 3544
EcoT38I GRGCYC 7 cut(s) 2005, 2177, 2547, 2969, 3516, 3912, 5075
ErhI CCWWGG 3 cut(s) 3351, 3516, 4626
Esp3I CGTCTC 2 cut(s) 4046, 4531
FalI AAGNNNNNCTT 2 cut(s) 2301, 2333
FaqI GGGAC 3 cut(s) 1062, 1996, 2316
FauI CCCGC 1 cut(s) 1856
FauNDI CATATG 2 cut(s) 3544, 3954
FbaI TGATCA 2 cut(s) 2929, 4651
FblI GTMKAC 2 cut(s) 372, 3522
FriOI GRGCYC 7 cut(s) 2005, 2177, 2547, 2969, 3516, 3912, 5075
FspBI CTAG 7 cut(s) 54, 872, 1367, 1400, 1781, 3707, 3935
GlaI GCGC 2 cut(s) 2328, 4296
GsaI CCCAGC 3 cut(s) 350, 724, 1239
GsuI CTGGAG 2 cut(s) 2161, 2825
HaeIII GGCC 9 cut(s) 5, 196, 523, 1817, 2846, 3356, 3689, 4750, 5073
HapII CCGG 7 cut(s) 773, 911, 3316, 3748, 3814, 4747, 4970
HgaI GACGC 2 cut(s) 2318, 4631
HhaI GCGC 2 cut(s) 2329, 4297
Hin1I GRCGYC 1 cut(s) 4623
Hin6I GCGC 2 cut(s) 2327, 4295
HinP1I GCGC 2 cut(s) 2327, 4295
HincII GTYRAC 3 cut(s) 163, 967, 1278
HindII GTYRAC 3 cut(s) 163, 967, 1278
HindIII AAGCTT 6 cut(s) 845, 936, 1379, 2315, 4007, 4508
HpaI GTTAAC 1 cut(s) 1278
HpaII CCGG 7 cut(s) 773, 911, 3316, 3748, 3814, 4747, 4970
HphI GGTGA 4 cut(s) 3517, 3578, 4661, 4897
Hpy99I CGWCG 1 cut(s) 195
HpyCH4IV ACGT 7 cut(s) 83, 571, 1321, 1449, 3439, 3946, 4540
HpySE526I ACGT 7 cut(s) 83, 571, 1321, 1449, 3439, 3946, 4540
Hsp92I GRCGYC 1 cut(s) 4623
HspAI GCGC 2 cut(s) 2327, 4295
Kpn2I TCCGGA 2 cut(s) 772, 910
KpnI GGTACC 1 cut(s) 3484
Ksp22I TGATCA 2 cut(s) 2929, 4651
KspAI GTTAAC 1 cut(s) 1278
LguI GCTCTTC 1 cut(s) 1389
MaeI CTAG 7 cut(s) 54, 872, 1367, 1400, 1781, 3707, 3935
MaeII ACGT 7 cut(s) 83, 571, 1321, 1449, 3439, 3946, 4540
MfeI CAATTG 2 cut(s) 1011, 1950
MflI RGATCY 7 cut(s) 757, 913, 1405, 1585, 2020, 2185, 2752
MlsI TGGCCA 1 cut(s) 1817
MluNI TGGCCA 1 cut(s) 1817
MlyI GAGTC 9 cut(s) 404, 1044, 1938, 1978, 2251, 3141, 3401, 3828, 4867
MmeI TCCRAC 5 cut(s) 696, 928, 1516, 1695, 3252
Mox20I TGGCCA 1 cut(s) 1817
Mph1103I ATGCAT 1 cut(s) 3544
MroI TCCGGA 2 cut(s) 772, 910
MscI TGGCCA 1 cut(s) 1817
MslI CAYNNNNRTG 7 cut(s) 29, 170, 176, 3408, 3587, 3851, 4148
Msp20I TGGCCA 1 cut(s) 1817
MspA1I CMGCKG 5 cut(s) 11, 932, 1111, 2219, 4400
MspI CCGG 7 cut(s) 773, 911, 3316, 3748, 3814, 4747, 4970
MunI CAATTG 2 cut(s) 1011, 1950
Mva1269I GAATGC 3 cut(s) 3311, 4799, 5064
MvaI CCWGG 9 cut(s) 507, 726, 1086, 1459, 1592, 2442, 2843, 3344, 3851
MvnI CGCG 2 cut(s) 2329, 2724
NciI CCSGG 3 cut(s) 3317, 3748, 4971
NcoI CCATGG 1 cut(s) 4626
NdeI CATATG 2 cut(s) 3544, 3954
NheI GCTAGC 1 cut(s) 1366
NlaIV GGNNCC 7 cut(s) 718, 915, 1115, 2187, 3341, 3482, 5073
NmuCI GTSAC 7 cut(s) 94, 355, 1317, 1783, 2254, 3566, 4343
NsiI ATGCAT 1 cut(s) 3544
PaeI GCATGC 1 cut(s) 1748
PagI TCATGA 2 cut(s) 3466, 4654
PaqCI CACCTGC 1 cut(s) 970
PasI CCCWGGG 1 cut(s) 1591
PciI ACATGT 2 cut(s) 2635, 4932
PciSI GCTCTTC 1 cut(s) 1389
PctI GAATGC 3 cut(s) 3311, 4799, 5064
PflMI CCANNNNNTGG 3 cut(s) 1998, 3427, 3617
PfoI TCCNGGA 2 cut(s) 2440, 4969
PleI GAGTC 9 cut(s) 403, 1044, 1937, 1978, 2251, 3140, 3400, 3828, 4867
PpsI GAGTC 9 cut(s) 403, 1044, 1937, 1978, 2251, 3140, 3400, 3828, 4867
Ppu21I YACGTR 1 cut(s) 3440
PpuMI RGGWCCY 1 cut(s) 2890
PscI ACATGT 2 cut(s) 2635, 4932
PshBI ATTAAT 3 cut(s) 66, 2340, 3915
PsiI TTATAA 1 cut(s) 4560
Psp124BI GAGCTC 2 cut(s) 2177, 3912
Psp5II RGGWCCY 1 cut(s) 2890
Psp6I CCWGG 9 cut(s) 505, 724, 1084, 1457, 1590, 2440, 2841, 3342, 3849
PspFI CCCAGC 3 cut(s) 346, 720, 1235
PspGI CCWGG 9 cut(s) 505, 724, 1084, 1457, 1590, 2440, 2841, 3342, 3849
PspN4I GGNNCC 7 cut(s) 718, 915, 1115, 2187, 3341, 3482, 5073
PspOMI GGGCCC 1 cut(s) 5071
PspPI GGNCC 6 cut(s) 2890, 3170, 3355, 3687, 5071, 5072
PspPPI RGGWCCY 1 cut(s) 2890
PstI CTGCAG 5 cut(s) 889, 1156, 1168, 3010, 4818
PstNI CAGNNNCTG 5 cut(s) 346, 884, 2987, 3094, 4948
PsuI RGATCY 7 cut(s) 757, 913, 1405, 1585, 2020, 2185, 2752
PvuII CAGCTG 3 cut(s) 932, 2219, 4400
RseI CAYNNNNRTG 7 cut(s) 29, 170, 176, 3408, 3587, 3851, 4148
SacI GAGCTC 2 cut(s) 2177, 3912
SapI GCTCTTC 1 cut(s) 1389
Sau96I GGNCC 6 cut(s) 2890, 3170, 3355, 3687, 5071, 5072
ScaI AGTACT 2 cut(s) 1185, 1262
SchI GAGTC 9 cut(s) 404, 1044, 1938, 1978, 2251, 3141, 3401, 3828, 4867
SfcI CTRYAG 9 cut(s) 885, 1152, 1164, 3006, 3086, 3192, 4228, 4464, 4814
SinI GGWCC 2 cut(s) 2890, 3170
SmiMI CAYNNNNRTG 7 cut(s) 29, 170, 176, 3408, 3587, 3851, 4148
SmlI CTYRAG 7 cut(s) 255, 2006, 2170, 3716, 4511, 4529, 4763
SmoI CTYRAG 7 cut(s) 255, 2006, 2170, 3716, 4511, 4529, 4763
SphI GCATGC 1 cut(s) 1748
SspI AATATT 1 cut(s) 2738
SspMI CTAG 7 cut(s) 54, 872, 1367, 1400, 1781, 3707, 3935
SstI GAGCTC 2 cut(s) 2177, 3912
StyI CCWWGG 3 cut(s) 3351, 3516, 4626
TaiI ACGT 7 cut(s) 86, 574, 1324, 1452, 3442, 3949, 4543
TaqI TCGA 5 cut(s) 233, 901, 2541, 3559, 4689
TatI WGTACW 7 cut(s) 114, 1183, 1260, 2148, 2632, 3701, 4830
TauI GCSGC 4 cut(s) 8, 11, 142, 3746
TscAI CASTG 7 cut(s) 383, 1000, 1425, 2185, 3532, 3880, 4091
TseFI GTSAC 7 cut(s) 94, 355, 1317, 1783, 2254, 3566, 4343
Tsp45I GTSAC 7 cut(s) 94, 355, 1317, 1783, 2254, 3566, 4343
TspGWI ACGGA 2 cut(s) 1178, 4340
TspRI CASTG 7 cut(s) 383, 1000, 1425, 2185, 3532, 3880, 4091
Van91I CCANNNNNTGG 3 cut(s) 1998, 3427, 3617
VneI GTGCAC 2 cut(s) 3948, 4238
VpaK11BI GGWCC 2 cut(s) 2890, 3170
VspI ATTAAT 3 cut(s) 66, 2340, 3915
XapI RAATTY 8 cut(s) 1211, 1502, 1684, 2824, 3058, 3809, 3825, 4124
XcmI CCANNNNNNNNNTGG 2 cut(s) 2854, 3424
XmiI GTMKAC 2 cut(s) 372, 3522
XspI CTAG 7 cut(s) 54, 872, 1367, 1400, 1781, 3707, 3935
ZrmI AGTACT 2 cut(s) 1185, 1262
Zsp2I ATGCAT 1 cut(s) 3544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.