MD01G1154700.v1.1

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002740
Physical Location & Seq
Forward (+)
26294653 .. 26304991
10339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1154700.v1.1.491

Sequence Viewer

Length: 5118 bp
ATGGCGGCCGCGAACGCTCCGATCACCATGAAGGAGGCCCTAACGCTTCCGAGCGTTGGGATTAATCCACAGTTCATAACGTTTACACATGTTACTATGGAATCCGATAAGTACATATGCGTTCGGGAGACTTCGCCTCAGAATAGTATTGTCATTATCGATATGAGTATGCCGATGCAACCTCTGAGGAGGCCTATCACTGCAGACTCTGCGCTTATGAATCCGAATTCCAAAATCCTTGCCTTGAAAGCTGGAGTTCAAGGTACTACTCAGGATCACCTTCAAATATTTAACATTGAGATGAAAGCAAAACTGAAATCTCATCTTATGCCTGAGCAGATTGTTTTCTGGAAGTGGATAACCCCAAAGATGTTGGGTTTAGTCACTCAGACTACGGTATATCACTGGTCAATTGAAGGTGAATCTGAACCTGTAAAGGTGTTTGAGCGAACAGCTAATCTGGCTAACAATCAGATTATAAATTACCGCTGCGATCCTTCTGAGAAGTGGTTGGTTTTGATTGGAATTGCTCCTGGTGCACCAGAGAGGCCACAATTGGTGAAAGGAAATTTGCAACTGTTCTCTGTGGATCAGCAGCGCAGTCAAGCTCTTGAAGCACATGCTGCATCATTTGCCCAATACAAGGTTCCAGGGAATGAGAATCCTTCAACTCTTATTTCTTTTGCCACCAAGACCCTCAATGCTGGACAGATTACATCAAAGTTACATGTTATTGAGCTTGGTGCCCAGCCAGGGAAACCATCTTTTACGAAGAAACAAGCAGATCTCTTCTTTCCTCCAGATTTTGCTGATGATTTCCCAGTCGCCATGCAGATGTCTCACAAGTACAGCTTGATTTATGTAATTACAAAGCTTGGGCTACTATTTGTGTATGATTTAGAGACAGCTAGTGCAGTTTACAGAAATAGAATTAGTCCGGATCCAATTTTCTTGACAACAGAGGCTTCATCAGTTGGAGGGTTTTATGCAGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACAATTAATGAACAAACAATTGTGCCTTTTGTCAGTGGTCAGCTAAACAATTTGGAGCTTGCTGTGAATCTGGCTAAAAGAGGAAATCTTCCAGGTGCCGAGAATCTGGTTGTCCAGCGTTTCCAAGAACTGTTTGCCCAAACAAAGTACAAAGAAGCTGCTGAGCTTGCAGCGGAGTCCCCACAAGGAATTCTCCGCACACCTGATACTGTTGCTAAATTCCAGAGTGTTCCTGTCCAAGCTGGGCAAACACCGCCTTTGTTGCAGTACTTTGGGACACTCTTGACAAGAGGGAAGCTCAATGCGTTTGAGTCATTGGAATTGTCACGTCTTGTAGTAAACCAGAACAAGAAGAACCTTTTAGAGAACTGGTTAGCAGAGGACAAACTCGAATGTAGTGAGGAATTGGGAGATCTTGTGAAGACTGTGGATAATGATCTGGCTTTGAAAATATATATTAAAGCCAGGGCAACTCCCAAAGTTGTTGCAGCTTTTGCTGAACGAAGGGAATTTGACAAAATTCTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTATCTGTTCCTTTTGCAAACAATTCTTCGAGCAGATCCTCAGGGTGCTGTTAATTTTGCATTAATGATGTCTCAAATGGAAGGTGGTTGTCCAGTTGATTACAACACCATAACGGATCTCTTCCTTCAGAGGAACCTTATTCGCGAGGCAACCGCCTTTTTATTGGATGTTTTGAAGCCAAATCTTCCAGAGCATGCGTTCCTGCAAACAAAGGTCCTTGAGATCAATTTAGTGACTTTCCCAAATGTGGCTGATGCTATTTTAGCCAATGGAATGTTCAGTCATTATGACCGTCCTCGTATTGCCCAGCTATGCGAAAAGGCTGGTCTTTATGTGCGAGCTTTGCAACATTACTCAGAGTTGCCTGATATTAAACGTGTCATAGTGAACACACATGCAATAGAGCCACAGTCACTTGTAGAGTTTTTTGGCACTCTATCAAAAGAATGGGCATTGGAGTGCATGAAAGATCTTTTATTGGTCAACTTAAGAGGAAACCTTCAGATAATAGTGCAGGTTGCTAAGGAATACTCTGAGCAGTTGGGAGTTGATCAGTGCATGAAGCTCTTTGAACAGTTCAAGTCATATGAAGGGTTGTATTTCTTCCTCGGTTCGTTTTTGAGCTCAAGTGAGGATCCTGATATCCACTTCAAGTACATTGAGGCAGCTGCTAAAACTGGACAAATCAAAGAGGTGGAGCGAGTTACTAGAGAATCAAACTTCTATGATGCTGAGAAGACGAAGAATTTCTTAATGGAAGCGAAGCTCCCCGATGCACGTCCATTGATTAATGTTTGTGATCGTTTTGGATTTGTTCCAGATCTCACCCACTACTTGTACACGAACAATATGCTTCGTTACATTGAAGGTTACGTTCAGAAGGTGAATCCTGGGAATGCTCCATTAGTTGTGGGACAGTTGCTGGATGATGAGTGCCCTGAAGATTTTATCAAAGGCTTAATTCTTTCTGTCCGTTCATTGCTTCCAGTGGAGCCCCTTGTGGACGAATGTGAGAAGAGGAATCGCCTTCGTTTACTCACACAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGATGTACATGTTCACAATGCTTTGGGTAAAATCATCATTGATAGCGGCAATAATCCAGAACACTTCCTTACCACAAACCCATATTATGATTCGCGTGTTGTTGGTAAATATTGTGAGAAACGTGATCCTACCCTGGCTGTTGTTGCTTACCGCCGTGGACAATGTGACGATGAACTCATTAATGTCACAAATAAGAACTCTTTGTTCAAATTGCAAGCAAGGTATGTGGTTGAGAGGATGGATGAAGATCTTTGGGGAAAAGTTCTTGACCCTGAGAATGAGTACAGAAGACAGCTTATTGATCAAGTTGTTTCTACTGCCTTGCCCGAAAGCAAGAGCCCGGAACAAGTTTCTGCGGCTGTTAAGGCTTTCATGACTGCTGATTTGCCCCATGAGTTAATTGAGCTTCTTGAAAAGATTGTGCTTCAAAACTCTGCCTTCAGTGGGAACTTTAATCTGCAAAACCTGCTAATCTTGACCGCCATCAAGGCAGATCCGTCGAGAGTCATGGATTACATTAATAGGCTTGATAATTTTGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCCCAACTGTATGAGGAAGCGTATGCAATCTTCAAGAAGTTTAACTTGAATGTCCAAGCGGTTAATGTCTTGCTGGATAACATCCGAAGCATTGATAGGGCTGTTGAGTTTGCGTTCCGTGTGGAAGAAGATGCTGTTTGGAGCCAGGTTGCAAAGGCTCAGCTCCGGGAGGGTCTGGTGAGCGATGCTATTGAGTCATTTATCCGTGCAGATGATGCCACTCAATTCTTGGATGTTATTCGCGCTTCTGAGGATGCAGATGTGTACCATGACTTGGTGAGGTATCTTCTGATGGTTAGGCAAAAGGTGAGAGAGCCCAAGGTGGACAGTGAGCTCATTTATGCATATGCAAAGATTGATAGGCTTGCTGACATTGAAGAATTTATTCTGATGCCCAATGTCGCTAATCTCCAAAATGTTGGTGATCGCCTATATGACGAGGCTCTCTATGAGGCTGCAAAGATAATATATGCCTTCATATCTAACTGGGCAAAGTTGGCTGTCACACTTGTGAAGCTCAAACAATTTCAAGGTGCTGTTGATGCAGCACGAAAAGCCAACAGTTCAAAGACATGGAAGGAGGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGCTTAGCTCAGATTTGTGGTCTCAACATTATTATACAGGTGGATGATCTGGAAGAGGTCAGTGAATACTACCAGAATAGAGGATGCTTCAACGAGCTAATATCTCTAATGGAGAGTGGTCTGGGGTTGGAACGTGCTCACATGGGAATCTTTACCGAGTTGGGGGTTCTATATGCTAGATATCGCCCAGAGAAACTTATGGAGCACATCAAATTGTTCGCAAATAGGCTAAATATTCCCAAGCTTATTAGAGCATGTGATGAGCAGCAACATTGGAAGGAGCTGACCTATCTGTATATTCAATATGATGAGTTTGATAATGCTGCAACCACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAGTTCAAAGACGTGGCAGTTAAAGTTGCTAACGTGGAGCTATATTATAAGGCTGTGCACTTCTACTTGCAAGAGCATCCGGACCTCATTAATGATATTCTAAATGTGCTTGCACTTCGTGTAGATCATACACGTGTGGTTGACATCATGCGAAAGGCTGGTCACTTGCTTCTTGTGAAGCCATACATGGTTGCAGTTCAGAGCAACAATGTCTCTGCTGTGAATGAGGCTTTGAACGAGATATATGTTGAGGAGGAAGACTATGAAAGATTGCGTGAATCCATTGATTTGCACGATAGCTTTGACCAAATAGGCCTCGCTCAAAAGATTGAGAAACACGAACTTCTGGAAATGAGACGTGTTGCTGCATATATCTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGCATTGTCAAAGAAAGATAAACTTTACAAAGATGCCATGGAGACAGCCTCACAATCTGGTGACCGTGAACTGGCAGAGGAGTTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCATCGTGCCTCTTTGTCTGTTACGATTTGATAAGGCCGGATATTGCCCTTGAACTTGCATGGATGAACAACATGATCGACTTTGCTTTCCCATATCTGTTACAGTTTATCCGGGAATACACTGGCAAAGTTGATGAACTGGTTAAGGACAAGATTGAAGCTCAAAAGGAGGTCAAGGCTAAAGAACAGGAAGATAAAGAAGTTATTGCGCAGCAGAACATGTATGCTCAGTTGCTGCCTCTTGCTTTGCCTGCGCCACCAATGCCTGGCATGGGAGGCGGAATGGGAGGAGGGTATGCTCCACCGCCACCTATGGGAGGAATGCCACCGATGGGCATGCCGCCCTTTGGCATGCCACCAATGGGCAGTAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1706

Amino Acids

193.09

Weight (kDa)

5.28

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 4.8e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 9.3e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 4e-28 Clathrin-H-link
Clathrin PF00637 557 - 692 7.9e-25 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 6.4e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 1.2e-32 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 4.4e-32 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 3.3e-30 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1432 1.1e-27 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 3.7e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 479, 4257
Acc16I TGCGCA 1 cut(s) 4950
Acc36I ACCTGC 4 cut(s) 1549, 2083, 3129, 3208
AccB1I GGYRCC 2 cut(s) 743, 1115
AccB7I CCANNNNNTGG 2 cut(s) 3499, 5006
AccII CGCG 4 cut(s) 11, 1722, 2749, 3468
AccIII TCCGGA 2 cut(s) 937, 4288
AclI AACGTT 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 8 cut(s) 226, 568, 1209, 1238, 1529, 1539, 2323, 3605
AcuI CTGAAG 5 cut(s) 1688, 2063, 2538, 3079, 4212
AcvI CACGTG 1 cut(s) 4343
AdeI CACNNNGTG 1 cut(s) 4328
AflII CTTAAG 1 cut(s) 2065
AflIII ACRYGT 8 cut(s) 88, 727, 1954, 2662, 4340, 4342, 4567, 4959
AjiI CACGTC 4 cut(s) 1349, 2357, 4222, 4568
AjuI GAANNNNNNNTTGG 4 cut(s) 630, 662, 1838, 1870
AleI CACNNNNGTG 1 cut(s) 3734
Alw21I GWGCWC 6 cut(s) 541, 2204, 3561, 3979, 4047, 4269
Alw26I GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
Alw44I GTGCAC 2 cut(s) 537, 4265
AlwNI CAGNNNCTG 3 cut(s) 209, 2500, 4975
Aor13HI TCCGGA 2 cut(s) 937, 4288
AoxI GGCC 7 cut(s) 6, 36, 191, 548, 1003, 4522, 4775
ApaLI GTGCAC 2 cut(s) 537, 4265
ApoI RAATTY 8 cut(s) 226, 568, 1209, 1238, 1529, 1539, 2323, 3605
ArsI GACNNNNNNTTYG 4 cut(s) 3022, 3054, 4354, 4386
AseI ATTAAT 7 cut(s) 63, 1026, 1640, 2367, 2835, 3174, 4299
Asp700I GAANNNNTTC 2 cut(s) 2324, 3609
AspLEI GCGC 5 cut(s) 214, 600, 3470, 4951, 4996
AspS9I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 4291
AsuC2I CCSGG 3 cut(s) 2996, 3392, 4853
AvaII GGWCC 3 cut(s) 1792, 3197, 4291
AxyI CCTNAGG 1 cut(s) 1617
BaeGI GKGCMC 4 cut(s) 541, 748, 2516, 4269
BamHI GGATCC 2 cut(s) 940, 2212
BanI GGYRCC 2 cut(s) 743, 1115
BanII GRGCYC 5 cut(s) 2204, 2574, 2996, 3543, 3561
BarI GAAGNNNNNNTAC 2 cut(s) 3239, 3271
BbrPI CACGTG 1 cut(s) 4343
BbsI GAAGAC 4 cut(s) 1448, 2321, 2950, 4473
Bbv12I GWGCWC 6 cut(s) 541, 2204, 3561, 3979, 4047, 4269
BbvCI CCTCAGC 1 cut(s) 3831
BccI CCATC 7 cut(s) 769, 2887, 3146, 3188, 3511, 4181, 5065
BceAI ACGGC 1 cut(s) 2793
BcgI CGANNNNNNTGC 6 cut(s) 2410, 2444, 3135, 3169, 5059, 5093
BclI TGATCA 2 cut(s) 2128, 2956
BcnI CCSGG 3 cut(s) 2996, 3392, 4853
BcoDI GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
BfaI CTAG 3 cut(s) 909, 2286, 4017
BfmI CTRYAG 2 cut(s) 201, 3201
BfrI CTTAAG 1 cut(s) 2065
BfuAI ACCTGC 4 cut(s) 1549, 2083, 3129, 3208
BglI GCCNNNNNGGC 1 cut(s) 3143
BglII AGATCT 5 cut(s) 784, 1432, 2047, 2398, 2902
BlpI GCTNAGC 3 cut(s) 1182, 3384, 3844
BmcAI AGTACT 1 cut(s) 1289
Bme18I GGWCC 3 cut(s) 1792, 3197, 4291
BmgBI CACGTC 4 cut(s) 1349, 2357, 4222, 4568
BmgT120I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 4291
BmiI GGNNCC 9 cut(s) 648, 745, 942, 1004, 1117, 1712, 2214, 2571, 3368
BmrI ACTGGG 2 cut(s) 815, 3721
BmuI ACTGGG 2 cut(s) 815, 3721
BpiI GAAGAC 4 cut(s) 1448, 2321, 2950, 4473
BplI GAGNNNNNCTC 4 cut(s) 1302, 1334, 4649, 4681
BpmI CTGGAG 2 cut(s) 273, 783
Bpu10I CCTNAGC 3 cut(s) 333, 2100, 3831
Bpu1102I GCTNAGC 3 cut(s) 1182, 3384, 3844
BpuEI CTTGAG 2 cut(s) 1817, 2188
BpuMI CCSGG 3 cut(s) 2996, 3392, 4853
Bsa29I ATCGAT 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 4343
BsaBI GATNNNNATC 2 cut(s) 1455, 2901
BsaI GGTCTC 1 cut(s) 3866
BsaWI WCCGGW 2 cut(s) 937, 4288
BsaXI ACNNNNNCTCC 2 cut(s) 1422, 1452
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 2 cut(s) 2555, 4614
Bse8I GATNNNNATC 2 cut(s) 1455, 2901
BseAI TCCGGA 2 cut(s) 937, 4288
BseCI ATCGAT 1 cut(s) 159
BseJI GATNNNNATC 2 cut(s) 1455, 2901
BseMI GCAATG 2 cut(s) 2555, 4614
BseRI GAGGAG 5 cut(s) 202, 3848, 4475, 4709, 5043
BseSI GKGCMC 4 cut(s) 541, 748, 2516, 4269
BseX3I CGGCCG 1 cut(s) 6
BseYI CCCAGC 3 cut(s) 747, 1262, 1884
BsgI GTGCAG 3 cut(s) 933, 2111, 3453
Bsh1236I CGCG 4 cut(s) 11, 1722, 2749, 3468
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 7 cut(s) 8, 38, 193, 550, 1005, 4524, 4777
BshNI GGYRCC 2 cut(s) 743, 1115
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 6 cut(s) 541, 2204, 3561, 3979, 4047, 4269
BsiSI CCGG 6 cut(s) 938, 2996, 3391, 4289, 4778, 4852
BslFI GGGAC 3 cut(s) 1183, 1309, 2505
BsmAI GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
BsmBI CGTCTC 1 cut(s) 4558
BsmFI GGGAC 3 cut(s) 1183, 1309, 2505
BsmI GAATGC 3 cut(s) 2479, 4739, 5067
BsnI GGCC 7 cut(s) 8, 38, 193, 550, 1005, 4524, 4777
Bso31I GGTCTC 1 cut(s) 3866
Bsp13I TCCGGA 2 cut(s) 937, 4288
Bsp1407I TGTACA 2 cut(s) 2415, 2659
Bsp1720I GCTNAGC 3 cut(s) 1182, 3384, 3844
Bsp19I CCATGG 1 cut(s) 4653
Bsp68I TCGCGA 1 cut(s) 1722
BspANI GGCC 7 cut(s) 8, 38, 193, 550, 1005, 4524, 4777
BspDI ATCGAT 1 cut(s) 159
BspEI TCCGGA 2 cut(s) 937, 4288
BspFNI CGCG 4 cut(s) 11, 1722, 2749, 3468
BspHI TCATGA 2 cut(s) 3027, 4176
BspLI GGNNCC 9 cut(s) 648, 745, 942, 1004, 1117, 1712, 2214, 2571, 3368
BspMAI CTGCAG 2 cut(s) 205, 3205
BspMI ACCTGC 4 cut(s) 1549, 2083, 3129, 3208
BspT107I GGYRCC 2 cut(s) 743, 1115
BspTI CTTAAG 1 cut(s) 2065
BspTNI GGTCTC 1 cut(s) 3866
BsrDI GCAATG 2 cut(s) 2555, 4614
BsrGI TGTACA 2 cut(s) 2415, 2659
BssT1I CCWWGG 2 cut(s) 3543, 4653
Bst6I CTCTTC 4 cut(s) 794, 1703, 2588, 3888
BstAFI CTTAAG 1 cut(s) 2065
BstAPI GCANNNNNTGC 6 cut(s) 209, 623, 632, 1514, 3121, 3440
BstAUI TGTACA 2 cut(s) 2415, 2659
BstBAI YACGTR 1 cut(s) 4343
BstDSI CCRYGG 2 cut(s) 2809, 4653
BstEII GGTNACC 1 cut(s) 4676
BstENI CCTNNNNNAGG 1 cut(s) 5055
BstFNI CGCG 4 cut(s) 11, 1722, 2749, 3468
BstHHI GCGC 5 cut(s) 214, 600, 3470, 4951, 4996
BstMAI GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
BstMCI CGRYCG 1 cut(s) 9
BstPI GGTNACC 1 cut(s) 4676
BstSFI CTRYAG 2 cut(s) 201, 3201
BstSLI GKGCMC 4 cut(s) 541, 748, 2516, 4269
BstUI CGCG 4 cut(s) 11, 1722, 2749, 3468
BstV2I GAAGAC 4 cut(s) 1448, 2321, 2950, 4473
BstXI CCANNNNNNTGG 1 cut(s) 3644
BstZI CGGCCG 1 cut(s) 6
Bsu15I ATCGAT 1 cut(s) 159
Bsu36I CCTNAGG 1 cut(s) 1617
BsuRI GGCC 7 cut(s) 8, 38, 193, 550, 1005, 4524, 4777
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 2 cut(s) 2809, 4653
BtgZI GCGATG 1 cut(s) 3423
BtrI CACGTC 4 cut(s) 1349, 2357, 4222, 4568
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 9 cut(s) 198, 403, 1060, 2138, 2571, 3103, 3559, 3907, 4860
BtuMI TCGCGA 1 cut(s) 1722
BveI ACCTGC 4 cut(s) 1549, 2083, 3129, 3208
CaiI CAGNNNCTG 3 cut(s) 209, 2500, 4975
CciI TCATGA 2 cut(s) 3027, 4176
CciNI GCGGCCGC 1 cut(s) 6
CfoI GCGC 5 cut(s) 214, 600, 3470, 4951, 4996
Cfr13I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 4291
ClaI ATCGAT 1 cut(s) 159
CspCI CAANNNNNGTGG 2 cut(s) 2862, 2897
DraIII CACNNNGTG 1 cut(s) 4328
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 4 cut(s) 794, 1703, 2588, 3888
EarI CTCTTC 4 cut(s) 794, 1703, 2588, 3888
EciI GGCGGA 1 cut(s) 5034
Ecl136II GAGCTC 2 cut(s) 2202, 3559
EclXI CGGCCG 1 cut(s) 6
Eco130I CCWWGG 2 cut(s) 3543, 4653
Eco147I AGGCCT 2 cut(s) 193, 4524
Eco24I GRGCYC 5 cut(s) 2204, 2574, 2996, 3543, 3561
Eco31I GGTCTC 1 cut(s) 3866
Eco32I GATATC 2 cut(s) 2221, 4022
Eco47I GGWCC 3 cut(s) 1792, 3197, 4291
Eco52I CGGCCG 1 cut(s) 6
Eco53kI GAGCTC 2 cut(s) 2202, 3559
Eco57I CTGAAG 5 cut(s) 1688, 2063, 2538, 3079, 4212
Eco72I CACGTG 1 cut(s) 4343
Eco81I CCTNAGG 1 cut(s) 1617
Eco91I GGTNACC 1 cut(s) 4676
EcoICRI GAGCTC 2 cut(s) 2202, 3559
EcoNI CCTNNNNNAGG 1 cut(s) 5055
EcoO109I RGGNCCY 2 cut(s) 37, 1792
EcoO65I GGTNACC 1 cut(s) 4676
EcoRI GAATTC 2 cut(s) 226, 1209
EcoRV GATATC 2 cut(s) 2221, 4022
EcoT14I CCWWGG 2 cut(s) 3543, 4653
EcoT22I ATGCAT 1 cut(s) 3571
EcoT38I GRGCYC 5 cut(s) 2204, 2574, 2996, 3543, 3561
ErhI CCWWGG 2 cut(s) 3543, 4653
Esp3I CGTCTC 1 cut(s) 4558
FalI AAGNNNNNCTT 8 cut(s) 836, 868, 2312, 2344, 3254, 3286, 4623, 4655
FaqI GGGAC 3 cut(s) 1183, 1309, 2505
FauI CCCGC 1 cut(s) 993
FauNDI CATATG 3 cut(s) 116, 2164, 3571
FbaI TGATCA 2 cut(s) 2128, 2956
FriOI GRGCYC 5 cut(s) 2204, 2574, 2996, 3543, 3561
FspBI CTAG 3 cut(s) 909, 2286, 4017
FspI TGCGCA 1 cut(s) 4950
GlaI GCGC 5 cut(s) 213, 599, 3469, 4950, 4995
GsaI CCCAGC 3 cut(s) 751, 1266, 1888
GsuI CTGGAG 2 cut(s) 273, 783
HaeIII GGCC 7 cut(s) 8, 38, 193, 550, 1005, 4524, 4777
HapII CCGG 6 cut(s) 938, 2996, 3391, 4289, 4778, 4852
HhaI GCGC 5 cut(s) 214, 600, 3470, 4951, 4996
Hin6I GCGC 5 cut(s) 212, 598, 3468, 4949, 4994
HinP1I GCGC 5 cut(s) 212, 598, 3468, 4949, 4994
HincII GTYRAC 2 cut(s) 2062, 4351
HindII GTYRAC 2 cut(s) 2062, 4351
HindIII AAGCTT 2 cut(s) 872, 4082
HpaII CCGG 6 cut(s) 938, 2996, 3391, 4289, 4778, 4852
Hpy99I CGWCG 1 cut(s) 3157
HspAI GCGC 5 cut(s) 212, 598, 3468, 4949, 4994
Kpn2I TCCGGA 2 cut(s) 937, 4288
Ksp22I TGATCA 2 cut(s) 2128, 2956
MaeI CTAG 3 cut(s) 909, 2286, 4017
MfeI CAATTG 3 cut(s) 411, 554, 1038
MlyI GAGTC 5 cut(s) 200, 1205, 1340, 3168, 3428
MmeI TCCRAC 2 cut(s) 955, 3948
Mph1103I ATGCAT 1 cut(s) 3571
MroI TCCGGA 2 cut(s) 937, 4288
MroXI GAANNNNTTC 2 cut(s) 2324, 3609
MslI CAYNNNNRTG 6 cut(s) 299, 833, 2035, 3734, 4175, 4341
MspA1I CMGCKG 3 cut(s) 489, 1193, 2246
MspCI CTTAAG 1 cut(s) 2065
MspI CCGG 6 cut(s) 938, 2996, 3391, 4289, 4778, 4852
MunI CAATTG 3 cut(s) 411, 554, 1038
Mva1269I GAATGC 3 cut(s) 2479, 4739, 5067
MvnI CGCG 4 cut(s) 11, 1722, 2749, 3468
NciI CCSGG 3 cut(s) 2996, 3392, 4853
NcoI CCATGG 1 cut(s) 4653
NdeI CATATG 3 cut(s) 116, 2164, 3571
NlaIV GGNNCC 9 cut(s) 648, 745, 942, 1004, 1117, 1712, 2214, 2571, 3368
NmeAIII GCCGAG 1 cut(s) 1144
NmuCI GTSAC 9 cut(s) 382, 1344, 1810, 1989, 2819, 2839, 3727, 4370, 4676
NotI GCGGCCGC 1 cut(s) 6
NruI TCGCGA 1 cut(s) 1722
NsbI TGCGCA 1 cut(s) 4950
NsiI ATGCAT 1 cut(s) 3571
OliI CACNNNNGTG 1 cut(s) 3734
PaeI GCATGC 3 cut(s) 1775, 5080, 5095
PagI TCATGA 2 cut(s) 3027, 4176
PceI AGGCCT 2 cut(s) 193, 4524
PciI ACATGT 4 cut(s) 88, 727, 2662, 4959
PctI GAATGC 3 cut(s) 2479, 4739, 5067
PdmI GAANNNNTTC 2 cut(s) 2324, 3609
PflMI CCANNNNNTGG 2 cut(s) 3499, 5006
PfoI TCCNGGA 2 cut(s) 3390, 4851
PleI GAGTC 5 cut(s) 200, 1204, 1339, 3167, 3427
PmaCI CACGTG 1 cut(s) 4343
PmlI CACGTG 1 cut(s) 4343
PpsI GAGTC 5 cut(s) 200, 1204, 1339, 3167, 3427
Ppu21I YACGTR 1 cut(s) 4343
PpuMI RGGWCCY 1 cut(s) 1792
PscI ACATGT 4 cut(s) 88, 727, 2662, 4959
PshBI ATTAAT 7 cut(s) 63, 1026, 1640, 2367, 2835, 3174, 4299
PsiI TTATAA 2 cut(s) 479, 4257
Psp124BI GAGCTC 2 cut(s) 2204, 3561
Psp1406I AACGTT 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 1792
PspCI CACGTG 1 cut(s) 4343
PspEI GGTNACC 1 cut(s) 4676
PspFI CCCAGC 3 cut(s) 747, 1262, 1884
PspN4I GGNNCC 9 cut(s) 648, 745, 942, 1004, 1117, 1712, 2214, 2571, 3368
PspPI GGNCC 5 cut(s) 37, 1003, 1792, 3197, 4291
PspPPI RGGWCCY 1 cut(s) 1792
PstI CTGCAG 2 cut(s) 205, 3205
PstNI CAGNNNCTG 3 cut(s) 209, 2500, 4975
PvuII CAGCTG 1 cut(s) 2246
RruI TCGCGA 1 cut(s) 1722
RseI CAYNNNNRTG 6 cut(s) 299, 833, 2035, 3734, 4175, 4341
SacI GAGCTC 2 cut(s) 2204, 3561
Sau96I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 4291
ScaI AGTACT 1 cut(s) 1289
SchI GAGTC 5 cut(s) 200, 1205, 1340, 3168, 3428
SfcI CTRYAG 2 cut(s) 201, 3201
SinI GGWCC 3 cut(s) 1792, 3197, 4291
SmiMI CAYNNNNRTG 6 cut(s) 299, 833, 2035, 3734, 4175, 4341
SmlI CTYRAG 3 cut(s) 1796, 2065, 2203
SmoI CTYRAG 3 cut(s) 1796, 2065, 2203
SphI GCATGC 3 cut(s) 1775, 5080, 5095
SseBI AGGCCT 2 cut(s) 193, 4524
SspI AATATT 3 cut(s) 288, 2765, 4075
SspMI CTAG 3 cut(s) 909, 2286, 4017
SstI GAGCTC 2 cut(s) 2204, 3561
StuI AGGCCT 2 cut(s) 193, 4524
StyI CCWWGG 2 cut(s) 3543, 4653
TaqI TCGA 5 cut(s) 159, 1410, 1606, 3155, 4818
TatI WGTACW 8 cut(s) 111, 846, 1167, 1287, 2232, 2415, 2659, 2937
TauI GCSGC 5 cut(s) 8, 11, 2703, 3014, 5083
TscAI CASTG 9 cut(s) 205, 410, 1060, 2138, 2571, 3103, 3559, 3907, 4867
TseFI GTSAC 9 cut(s) 382, 1344, 1810, 1989, 2819, 2839, 3727, 4370, 4676
Tsp45I GTSAC 9 cut(s) 382, 1344, 1810, 1989, 2819, 2839, 3727, 4370, 4676
TspGWI ACGGA 5 cut(s) 1706, 2540, 3141, 3332, 3419
TspRI CASTG 9 cut(s) 205, 410, 1060, 2138, 2571, 3103, 3559, 3907, 4867
Van91I CCANNNNNTGG 2 cut(s) 3499, 5006
Vha464I CTTAAG 1 cut(s) 2065
VneI GTGCAC 2 cut(s) 537, 4265
VpaK11BI GGWCC 3 cut(s) 1792, 3197, 4291
VspI ATTAAT 7 cut(s) 63, 1026, 1640, 2367, 2835, 3174, 4299
XagI CCTNNNNNAGG 1 cut(s) 5055
XapI RAATTY 8 cut(s) 226, 568, 1209, 1238, 1529, 1539, 2323, 3605
XcmI CCANNNNNNNNNTGG 1 cut(s) 3451
XmnI GAANNNNTTC 2 cut(s) 2324, 3609
XspI CTAG 3 cut(s) 909, 2286, 4017
ZrmI AGTACT 1 cut(s) 1289
Zsp2I ATGCAT 1 cut(s) 3571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.