Rh5CG503100

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0002740
Physical Location & Seq
Forward (+)
69879715 .. 69890963
11249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG503100.1

Sequence Viewer

Length: 5115 bp
ATGGCCGCCGCTGCCAATGCCCCCATGACCATGAAAGAGCAGCTCACGCTGCCTAGTATTGGGATTAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGCGTTAGGGAGACCTCGCCGCAGAATAGCATCGTGATCGTTGACATGAACATGCCAATGCAGCCGTTGCGACGGCCGATTACTGCTGATTCCGCTTTGATGAATCCTAATTCGAGGATTCTGGCTCTTAAAGCTCAAGTGCAAGGAACTACTCAGGATCATTTACAGATATTTAACATTGAACTGAAAACAAAGATGAAATCACATCAGATGCCTGAGCAGATCGTGTTTTGGAAGTGGGTCACTCCTAAGATGCTGGGGATGGTCACACAGACCTCAGTATACCACTGGTTAATTGACGGGGAGTCTGAGCCAACAAAGGTGTTTGAGCGTACAGCTAATTTAGCAAATAATCAAATTATCAACTACCGTTGTGATCCCTCTGAAAAGTGGTTGGTCTTAATTGGAATTGCTCCTGGTTCTCCTGAGAGGCCACAACTGGTTAAAGGAAACATGCAGCTTTTCTCTGTGGAGCAGCAACGTAGTCAGGCTCTTGAAGCACATGCTGCATCATTTGCCCCATTTAAAGTTGCAGGGAACGAGAACCCTTCAATTCTTATCTGCTTTGCTGCAAAGTCCTCCAACGCTGGACAAGTTACATCAAAGTTGCATGTTATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATATACTAAGAAGCAAGCAGATCTATTCTTTCCTCCGGATTTTGCTGATGACTTTCCTGTGTCAATGCAGATATCAGAAAAGTATGGGTTGATATATGTTATTACAAAGCTTGGACTACTGTTTGTATATGATCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCGGATCCTATATTTTTGACAGCTGAAGCTTCATCTGTTGGAGGTTTTTATGCAGTCAACAGGCGTGGTCAGGTGTTACTTGCCACTGTAAATGAACAAACAATTGTACCTTTTGTTAGTGGTCAGTTAAACAATTTGGGACTCGCAGTTAGTCTTGCCAGAAGAGGAAATCTTCCTGGTGCTGAAGACTTGGTTGTCCAGCGGTTCCAAGAATTGTTTTCTCAGACAAAATATAAGGAGGCTGCAGAGCTTGCTGCAGATTCTCCAATGGGAGTACTCCGTACACCTGAAACTGTTGCTAAATTTCAGGGTGTTCCTGTGCAAGCTGGGCAAACACCACCATTGTTGCAGTACTTTGGAACTTTGTTAACTAAAGGGAAACTCAACGCTTTTGAATCATTGGAACTGTCACGTTTGGTTGTAAATCAAAACAAAAAGAATCTTTTGGAGAATTGGCTAGCGGAGGACAAGCTTGAATGTAGCGAAGAGCTAGGAGATCTTGTGAAGACGGTGGATAATGACCTTGCTTTGAAAATATACGTCAAAGCCAGGGCAACTCCAAAAGTTGTTGCAGCCTTTGCTGAGAGAAGGGAATTTGACAAGATTTTAATATACTCGAAACAGGTTGGATATACACCTGATTATATGTTCCTTCTGCAAACAATTTTGCGGACAGATCCCCAGGGGGCGGTCAATTTTGCACTAATGATGTCTCAAATGGAAGGAGGTTGCCCTATTGACTACAATACCATTACTGATCTGTTCCTTCAGAGGAATTTGATTCGTGAGGCAACAGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCTGAGCATGCCCACCTTCAGACAAAGGTTTTGGAAATCAATCTAGTGACTTTTCCCAATGTTGCGGATGCTATATTGGCCAATGGTATGTTCAGTCATTATGACCGCCCCCGCATTGGTCAGCTCTGTGAAAAAGCTGGTTTGTATTTGCGTGCCTTACAGCATTATACCGAGTTACCTGATATTAAAAGAGTCATTGTGAACACTCATGCAATTGACCCTCAGGCTCTTGTTGAGTTCTTCGGGACTCTTTCCATAGAATGGGCTCTTGAGTGCATGAAAGATCTTCTACTGGTCAATCTTAGGGGCAATCTTCAGATCATTGTGCAGACTGCCAAAGAGTATTCCGAGCAACTGGGTGTAGATAAATGTATAAAGTTGTTTGAGCAGTTCAAGTCCTATGAGGGGTTGTACTTTTTCTTAGGCTCATACTTGAGCTCCAGTGAGGATCCCGATATCCACTTTAAGTATATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGAGTGACTCGTGAATCTAATTTTTATGATGCTGAGAAGGCTAAGAACTTTCTGATGGAAGCAAAGCTTCCAGATGCGCGTCCCCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCTGATCTCACTCATTACCTATATACGAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTTGGGCAGCTTTTGGATGATGAATGTCCTGAAGATTTCATCAAAGGACTTATTCTATCAGTTCGTTCTCTTCTACCAGTCGAGCCCCTTGTTGATGAATGTGAAAAGAGGAATCGTCTTCGCCTGCTCACTCAGTTCTTGGAGCATCTTGTGAGTGAAGGAAGCCAAGATGTACATGTTCATAATGCTCTTGGTAAAATCATCATAGATAGTGGCAACAATCCAGAGCATTTTCTCACAACTAACCCATATTATGATTCTCGTGTTGTGGGTAAATATTGTGAAAAAAGAGATCCCACCCTTGCTGTTGTTGCTTACCGTAGAGGGCAATGTGATGATGAACTTGTTAATGTAACAAATAAGAATTCATTATTCAAACTCCAGGCCAGATATGTTGTGGAAAGAATGGATGATGACCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAGTATAGAAGGCAGCTAATTGATCAAGTTGTCTCTACTGCTTTGCCTGAAAGCAAGAGCCCTGAGCAGGTTTCAGCAACTGTTAAAGCGTTTATGACTGCGGACCTTCCACATGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTCCAAAATTCAGCTTTTAGTGGGAACTTCAATCTACAGAATCTGTTGATCTTGACTGCAATTAAGGCTGATCCATCCAGAGTCATGGATTATATCAATAGATTGGACAACTTTGATGGGCCTGCGGTTGGCGTAATGGCTGTAGAAGCCCAACTCTATGAGGAAGCATTTGCAATCTTTAAGAAGTTCAATTTGAATGTTGATGCTGTCAATGTTCTGTTGGATAACATTCAGAGCATTGACCGTGCCGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTTGCCAAGGCCCAACTACGAGAGGGGCTTGTGAGTGATGCTATTGAGTCATTCATTCGTGCTGATGATGCCACCCAGTTTTTGGATGTTATACGTGCTGCTCAGGATGCAGATGTGTATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGGTGAAAGAGCCCAAGGTAGACAGTGAACTCATTTATGCATATGCTAAGATTGATCGACTTGGTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCTAATCTCCCAAATGTTGGGGATCGCCTGTATGATGAAGAACTATATGAAGCAGCCAAGATCATATTTGCATTTATTTCTAATTGGGCCAAGCTGGCGTGTACTCTAGTGAAGCTCAAGCAGTTTCAAGGTGCTGTTGATGCTGCCCGGAAAGCTAACAGCTCAAAAACTTGGAAGGAAGTTTGCTTTGCATGTGTTGATGCTGAGGAATTCCGGTTGGCTCAAATTTGTGGACTCAACATCATCATCCAGGTGGATGATTTGGAAGAGGTCAGTGAGTTTTACCAGAACAGGGGTTGCTTTAATGAGCTCATTAATCTTATGGAGAGTGGACTAGGATTGGAACGTGCACATATGGGCATTTTTACTGAATTAGGAGTTCTATATGCAAGATACCGTCCTGAGAAGCTTATGGAGCACATTAAACTGTTTTCTACTCGTCTCAACATTCCCAAACTTATACGAGCTTGTGATGAACAGCAGCACTGGAAGGAATTAACATACTTATATATACAATACGATGAATTTGATAATGCTGCAACCACCATAATGAACCACTCCCCAGAAGCATGGGAACACATGCAATTCAAGGATGTGATCGTCAAAGTTGCAAGTGTTGAGCTTTACTATAGGGCTGTGCACTTTTACTTAGAAGAACATCCTGATATTATCAATGATGTTCTAAATGTGCTTGCGCTTCGTGTGGATCATACCCGAGTGGTGGATATAATGCGAAAGGCTGGTCACATCCGTCTGGTAAAGCCATACATGGTTGCAGTCCAGAGCAACAATGTAACAGCTGTAAATGAAGCTCTAAATGAAATATATATTGAGGAGGAAGACTATGACAGACTTCGTGAATCTATAGATTTGCATGATAGCTTTGACCAGATAGGTCTTGCACAAAAGCTTGAGAAACATGAGCTTCTTGAGATGAGACGTGTTGCTGCCTACATTTATAAGAAAGCTGGAAGATGGAAGCAATCCATTGCATTGTCAAAGAAAGACAAACATTACAAAGACGCCATGGAGACATGCTCACAGTCTGGTGATCATGAACTTTCAGAGGAATTGCTTGTCTATTTCATCGAGCAGGGAAAGAAAGAATGTTTCGCTTCGTGTCTCTTTGTTTGTTTTGATTTAATCCGGCCTGATGTTGCTCTTGAGCTGGCATGGATGAACAATATAATTGATTTTGCATTCCCATATATCCTGCAGTTTATTCGTGAATACACAAGCAAGGTTGATGAACTTGTCAAAGACAAAATTGAGACTCAGAAAGAGGTGAAAGCCAAAGAGCAAGAGGAGAAGGAATTGGTTGCTAAGCAGAACATGTATGCTCAGTTGCTGCCTGCTTTGCCTGCACCTCCGGGAATGGGTGGAGGTTACGCTCCACCTCCAATGCCACCAATGCCACAGATGGGTGGGATGGGGATGCCTCCAATGCCACCTTTCGGAATGCCACAAATGGGCCCTTCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1704

Amino Acids

193.22

Weight (kDa)

5.28

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 21 - 57 2.5e-06 Clathrin propeller repeat
Clathrin-link PF09268 345 - 367 6.2e-08 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 370 - 435 1.2e-26 Clathrin-H-link
Clathrin PF00637 558 - 693 6.4e-25 Region in Clathrin and VPS
Clathrin PF00637 702 - 842 7.2e-27 Region in Clathrin and VPS
Clathrin PF00637 851 - 985 2.3e-32 Region in Clathrin and VPS
Clathrin PF00637 994 - 1132 3e-32 Region in Clathrin and VPS
Clathrin PF00637 1145 - 1282 1.5e-29 Region in Clathrin and VPS
Clathrin PF00637 1291 - 1432 7.7e-26 Region in Clathrin and VPS
Clathrin PF00637 1439 - 1580 8.3e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4590
AasI GACNNNNNNGTC 1 cut(s) 1133
Acc36I ACCTGC 1 cut(s) 2995
Acc65I GGTACC 1 cut(s) 3510
AccB1I GGYRCC 2 cut(s) 746, 3510
AccB7I CCANNNNNTGG 3 cut(s) 2028, 3457, 3647
AccI GTMKAC 2 cut(s) 402, 3552
AccII CGCG 1 cut(s) 2359
AccIII TCCGGA 2 cut(s) 802, 940
AcoI YGGCCR 3 cut(s) 3, 194, 1845
AcsI RAATTY 8 cut(s) 1241, 1532, 1714, 2854, 3088, 3839, 3855, 4154
AcuI CTGAAG 7 cut(s) 984, 1143, 1691, 1769, 1775, 2066, 2541
AcyI GRCGYC 1 cut(s) 4653
AflIII ACRYGT 3 cut(s) 2665, 4570, 4962
AhdI GACNNNNNGTC 1 cut(s) 424
AjiI CACGTC 1 cut(s) 4571
AjnI CCWGG 9 cut(s) 535, 754, 1114, 1487, 1620, 2470, 2871, 3372, 3879
AjuI GAANNNNNNNTTGG 2 cut(s) 1841, 1873
Alw21I GWGCWC 6 cut(s) 2207, 3084, 3942, 3982, 4050, 4272
Alw26I GTCTC 9 cut(s) 125, 899, 1656, 2974, 4076, 4561, 4655, 4757, 4895
Alw44I GTGCAC 2 cut(s) 3978, 4268
AlwNI CAGNNNCTG 4 cut(s) 914, 3017, 3124, 4978
Ama87I CYCGRG 1 cut(s) 4344
Aor13HI TCCGGA 2 cut(s) 802, 940
ApaI GGGCCC 1 cut(s) 5105
ApaLI GTGCAC 2 cut(s) 3978, 4268
ApoI RAATTY 8 cut(s) 1241, 1532, 1714, 2854, 3088, 3839, 3855, 4154
ArsI GACNNNNNNTTYG 4 cut(s) 1780, 1812, 4357, 4389
AseI ATTAAT 3 cut(s) 66, 2370, 3945
Asp718I GGTACC 1 cut(s) 3510
AspLEI GCGC 2 cut(s) 2359, 4327
AspS9I GGNCC 7 cut(s) 2920, 3040, 3200, 3385, 3717, 5101, 5102
AsuC2I CCSGG 3 cut(s) 3347, 3778, 5001
AsuHPI GGTGA 4 cut(s) 3547, 3608, 4691, 4927
AsuNHI GCTAGC 1 cut(s) 1396
AvaI CYCGRG 1 cut(s) 4344
AvaII GGWCC 2 cut(s) 2920, 3040
AxyI CCTNAGG 1 cut(s) 1989
BaeGI GKGCMC 4 cut(s) 751, 3982, 4272, 5105
BaeI ACNNNNGTAYC 2 cut(s) 1029, 1062
BalI TGGCCA 1 cut(s) 1847
BamHI GGATCC 2 cut(s) 943, 2215
BanI GGYRCC 2 cut(s) 746, 3510
BanII GRGCYC 7 cut(s) 2035, 2207, 2577, 2999, 3546, 3942, 5105
BarI GAAGNNNNNNTAC 2 cut(s) 3498, 3530
BauI CACGAG 2 cut(s) 2289, 2751
BbsI GAAGAC 4 cut(s) 1131, 1451, 2600, 4476
Bbv12I GWGCWC 6 cut(s) 2207, 3084, 3942, 3982, 4050, 4272
BbvCI CCTCAGC 1 cut(s) 3834
BccI CCATC 9 cut(s) 376, 772, 2329, 3163, 3191, 3514, 4599, 5044, 5053
BceAI ACGGC 3 cut(s) 169, 209, 3314
BcgI CGANNNNNNTGC 6 cut(s) 139, 173, 2413, 2447, 4835, 4869
BciT130I CCWGG 9 cut(s) 537, 756, 1116, 1489, 1622, 2472, 2873, 3374, 3881
BclI TGATCA 2 cut(s) 2959, 4681
BcnI CCSGG 3 cut(s) 3347, 3778, 5001
BcoDI GTCTC 9 cut(s) 125, 899, 1656, 2974, 4076, 4561, 4655, 4757, 4895
BfaI CTAG 7 cut(s) 54, 902, 1397, 1430, 1811, 3737, 3965
BfmI CTRYAG 8 cut(s) 915, 1182, 1194, 3116, 3222, 4258, 4494, 4844
BfuAI ACCTGC 1 cut(s) 2995
BglI GCCNNNNNGGC 1 cut(s) 3725
BglII AGATCT 3 cut(s) 787, 1435, 2050
BlpI GCTNAGC 1 cut(s) 4953
BmcAI AGTACT 2 cut(s) 1215, 1292
Bme18I GGWCC 2 cut(s) 2920, 3040
BmeRI GACNNNNNGTC 1 cut(s) 424
BmeT110I CYCGRG 1 cut(s) 4344
BmgBI CACGTC 1 cut(s) 4571
BmgT120I GGNCC 7 cut(s) 2920, 3040, 3200, 3385, 3717, 5101, 5102
BmiI GGNNCC 7 cut(s) 748, 945, 1145, 2217, 3371, 3512, 5103
BmrI ACTGGG 2 cut(s) 2132, 3445
BmtI GCTAGC 1 cut(s) 1400
BmuI ACTGGG 2 cut(s) 2132, 3445
BpiI GAAGAC 4 cut(s) 1131, 1451, 2600, 4476
BplI GAGNNNNNCTC 2 cut(s) 4652, 4684
BpmI CTGGAG 2 cut(s) 2191, 2855
Bpu10I CCTNAGC 6 cut(s) 336, 1770, 2916, 3000, 3477, 3834
Bpu1102I GCTNAGC 1 cut(s) 4953
BpuEI CTTGAG 7 cut(s) 240, 2057, 2221, 3731, 4562, 4580, 4814
BpuMI CCSGG 3 cut(s) 3347, 3778, 5001
BsaAI YACGTR 1 cut(s) 3470
BsaBI GATNNNNATC 1 cut(s) 3492
BsaHI GRCGYC 1 cut(s) 4653
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 6 cut(s) 1488, 1620, 1621, 3381, 3546, 4656
BsaWI WCCGGW 3 cut(s) 802, 940, 3843
BsaXI ACNNNNNCTCC 4 cut(s) 1425, 1455, 2189, 2219
Bse1I ACTGG 9 cut(s) 413, 564, 2064, 2127, 2208, 2263, 2567, 3451, 4121
Bse21I CCTNAGG 1 cut(s) 1989
Bse3DI GCAATG 2 cut(s) 2825, 4617
Bse8I GATNNNNATC 1 cut(s) 3492
BseAI TCCGGA 2 cut(s) 802, 940
BseBI CCWGG 9 cut(s) 537, 756, 1116, 1489, 1622, 2472, 2873, 3374, 3881
BseDI CCNNGG 6 cut(s) 1488, 1620, 1621, 3381, 3546, 4656
BseJI GATNNNNATC 1 cut(s) 3492
BseMI GCAATG 2 cut(s) 2825, 4617
BseNI ACTGG 9 cut(s) 413, 564, 2064, 2127, 2208, 2263, 2567, 3451, 4121
BseRI GAGGAG 2 cut(s) 4478, 4949
BseSI GKGCMC 4 cut(s) 751, 3982, 4272, 5105
BseX3I CGGCCG 1 cut(s) 194
BseYI CCCAGC 3 cut(s) 376, 750, 1265
BsgI GTGCAG 2 cut(s) 2114, 4977
Bsh1236I CGCG 1 cut(s) 2359
Bsh1285I CGRYCG 1 cut(s) 197
BshNI GGYRCC 2 cut(s) 746, 3510
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 6 cut(s) 2207, 3084, 3942, 3982, 4050, 4272
BsiHKCI CYCGRG 1 cut(s) 4344
BsiSI CCGG 7 cut(s) 803, 941, 3346, 3778, 3844, 4777, 5000
BslFI GGGAC 3 cut(s) 1092, 2026, 2346
BsmAI GTCTC 9 cut(s) 125, 899, 1656, 2974, 4076, 4561, 4655, 4757, 4895
BsmBI CGTCTC 2 cut(s) 4076, 4561
BsmFI GGGAC 3 cut(s) 1092, 2026, 2346
BsmI GAATGC 3 cut(s) 3341, 4829, 5094
Bso31I GGTCTC 1 cut(s) 125
BsoBI CYCGRG 1 cut(s) 4344
Bsp120I GGGCCC 1 cut(s) 5101
Bsp13I TCCGGA 2 cut(s) 802, 940
Bsp1407I TGTACA 1 cut(s) 2662
Bsp1720I GCTNAGC 1 cut(s) 4953
Bsp19I CCATGG 1 cut(s) 4656
BspEI TCCGGA 2 cut(s) 802, 940
BspFNI CGCG 1 cut(s) 2359
BspHI TCATGA 2 cut(s) 3496, 4684
BspLI GGNNCC 7 cut(s) 748, 945, 1145, 2217, 3371, 3512, 5103
BspMAI CTGCAG 4 cut(s) 919, 1186, 1198, 4848
BspMI ACCTGC 1 cut(s) 2995
BspOI GCTAGC 1 cut(s) 1400
BspQI GCTCTTC 1 cut(s) 1419
BspT107I GGYRCC 2 cut(s) 746, 3510
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 2825, 4617
BsrGI TGTACA 1 cut(s) 2662
BsrI ACTGG 9 cut(s) 413, 564, 2064, 2127, 2208, 2263, 2567, 3451, 4121
BssECI CCNNGG 6 cut(s) 1488, 1620, 1621, 3381, 3546, 4656
BssNAI GTATAC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 4653
BssSI CACGAG 2 cut(s) 2289, 2751
BssT1I CCWWGG 3 cut(s) 3381, 3546, 4656
Bst1107I GTATAC 1 cut(s) 403
Bst2BI CACGAG 2 cut(s) 2289, 2751
Bst2UI CCWGG 9 cut(s) 537, 756, 1116, 1489, 1622, 2472, 2873, 3374, 3881
Bst6I CTCTTC 5 cut(s) 1096, 1419, 2565, 3600, 3891
BstACI GRCGYC 1 cut(s) 4653
BstAPI GCANNNNNTGC 5 cut(s) 187, 626, 635, 1190, 1517
BstAUI TGTACA 1 cut(s) 2662
BstBAI YACGTR 1 cut(s) 3470
BstDSI CCRYGG 1 cut(s) 4656
BstFNI CGCG 1 cut(s) 2359
BstHHI GCGC 2 cut(s) 2359, 4327
BstMAI GTCTC 9 cut(s) 125, 899, 1656, 2974, 4076, 4561, 4655, 4757, 4895
BstMCI CGRYCG 1 cut(s) 197
BstNI CCWGG 9 cut(s) 537, 756, 1116, 1489, 1622, 2472, 2873, 3374, 3881
BstSFI CTRYAG 8 cut(s) 915, 1182, 1194, 3116, 3222, 4258, 4494, 4844
BstSLI GKGCMC 4 cut(s) 751, 3982, 4272, 5105
BstUI CGCG 1 cut(s) 2359
BstV2I GAAGAC 4 cut(s) 1131, 1451, 2600, 4476
BstX2I RGATCY 7 cut(s) 787, 943, 1435, 1615, 2050, 2215, 2782
BstXI CCANNNNNNTGG 2 cut(s) 3166, 4200
BstYI RGATCY 7 cut(s) 787, 943, 1435, 1615, 2050, 2215, 2782
BstZ17I GTATAC 1 cut(s) 403
BstZI CGGCCG 1 cut(s) 194
Bsu36I CCTNAGG 1 cut(s) 1989
BtgI CCRYGG 1 cut(s) 4656
BtrI CACGTC 1 cut(s) 4571
BtsIMutI CAGTG 6 cut(s) 406, 1023, 2215, 3562, 3910, 4114
BveI ACCTGC 1 cut(s) 2995
CaiI CAGNNNCTG 4 cut(s) 914, 3017, 3124, 4978
CciI TCATGA 2 cut(s) 3496, 4684
CfoI GCGC 2 cut(s) 2359, 4327
Cfr13I GGNCC 7 cut(s) 2920, 3040, 3200, 3385, 3717, 5101, 5102
CseI GACGC 2 cut(s) 2348, 4661
CspCI CAANNNNNGTGG 6 cut(s) 985, 1020, 1266, 1301, 3036, 3071
DraI TTTAAA 1 cut(s) 646
DrdI GACNNNNNNGTC 1 cut(s) 1133
DriI GACNNNNNGTC 1 cut(s) 424
DseDI GACNNNNNNGTC 1 cut(s) 1133
EaeI YGGCCR 3 cut(s) 3, 194, 1845
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 5 cut(s) 1096, 1419, 2565, 3600, 3891
Eam1105I GACNNNNNGTC 1 cut(s) 424
EarI CTCTTC 5 cut(s) 1096, 1419, 2565, 3600, 3891
Ecl136II GAGCTC 2 cut(s) 2205, 3940
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 3 cut(s) 3381, 3546, 4656
Eco24I GRGCYC 7 cut(s) 2035, 2207, 2577, 2999, 3546, 3942, 5105
Eco31I GGTCTC 1 cut(s) 125
Eco32I GATATC 2 cut(s) 840, 2224
Eco47I GGWCC 2 cut(s) 2920, 3040
Eco52I CGGCCG 1 cut(s) 194
Eco53kI GAGCTC 2 cut(s) 2205, 3940
Eco57I CTGAAG 7 cut(s) 984, 1143, 1691, 1769, 1775, 2066, 2541
Eco81I CCTNAGG 1 cut(s) 1989
Eco88I CYCGRG 1 cut(s) 4344
EcoICRI GAGCTC 2 cut(s) 2205, 3940
EcoO109I RGGNCCY 2 cut(s) 2920, 5102
EcoRI GAATTC 2 cut(s) 2854, 3839
EcoRII CCWGG 9 cut(s) 535, 754, 1114, 1487, 1620, 2470, 2871, 3372, 3879
EcoRV GATATC 2 cut(s) 840, 2224
EcoT14I CCWWGG 3 cut(s) 3381, 3546, 4656
EcoT22I ATGCAT 1 cut(s) 3574
EcoT38I GRGCYC 7 cut(s) 2035, 2207, 2577, 2999, 3546, 3942, 5105
ErhI CCWWGG 3 cut(s) 3381, 3546, 4656
Esp3I CGTCTC 2 cut(s) 4076, 4561
FalI AAGNNNNNCTT 2 cut(s) 2331, 2363
FaqI GGGAC 3 cut(s) 1092, 2026, 2346
FauI CCCGC 1 cut(s) 1886
FauNDI CATATG 2 cut(s) 3574, 3984
FbaI TGATCA 2 cut(s) 2959, 4681
FblI GTMKAC 2 cut(s) 402, 3552
FriOI GRGCYC 7 cut(s) 2035, 2207, 2577, 2999, 3546, 3942, 5105
FspBI CTAG 7 cut(s) 54, 902, 1397, 1430, 1811, 3737, 3965
GlaI GCGC 2 cut(s) 2358, 4326
GsaI CCCAGC 3 cut(s) 380, 754, 1269
GsuI CTGGAG 2 cut(s) 2191, 2855
HapII CCGG 7 cut(s) 803, 941, 3346, 3778, 3844, 4777, 5000
HgaI GACGC 2 cut(s) 2348, 4661
HhaI GCGC 2 cut(s) 2359, 4327
Hin1I GRCGYC 1 cut(s) 4653
Hin6I GCGC 2 cut(s) 2357, 4325
HinP1I GCGC 2 cut(s) 2357, 4325
HincII GTYRAC 3 cut(s) 163, 997, 1308
HindII GTYRAC 3 cut(s) 163, 997, 1308
HindIII AAGCTT 6 cut(s) 875, 966, 1409, 2345, 4037, 4538
HpaI GTTAAC 1 cut(s) 1308
HpaII CCGG 7 cut(s) 803, 941, 3346, 3778, 3844, 4777, 5000
HphI GGTGA 4 cut(s) 3547, 3608, 4691, 4927
Hpy99I CGWCG 1 cut(s) 195
HpyCH4IV ACGT 7 cut(s) 83, 601, 1351, 1479, 3469, 3976, 4570
HpySE526I ACGT 7 cut(s) 83, 601, 1351, 1479, 3469, 3976, 4570
Hsp92I GRCGYC 1 cut(s) 4653
HspAI GCGC 2 cut(s) 2357, 4325
Kpn2I TCCGGA 2 cut(s) 802, 940
KpnI GGTACC 1 cut(s) 3514
Ksp22I TGATCA 2 cut(s) 2959, 4681
KspAI GTTAAC 1 cut(s) 1308
LguI GCTCTTC 1 cut(s) 1419
MaeI CTAG 7 cut(s) 54, 902, 1397, 1430, 1811, 3737, 3965
MaeII ACGT 7 cut(s) 83, 601, 1351, 1479, 3469, 3976, 4570
MfeI CAATTG 2 cut(s) 1041, 1980
MflI RGATCY 7 cut(s) 787, 943, 1435, 1615, 2050, 2215, 2782
MlsI TGGCCA 1 cut(s) 1847
MluNI TGGCCA 1 cut(s) 1847
MlyI GAGTC 9 cut(s) 434, 1074, 1968, 2008, 2281, 3171, 3431, 3858, 4897
MmeI TCCRAC 5 cut(s) 726, 958, 1546, 1725, 3282
Mox20I TGGCCA 1 cut(s) 1847
Mph1103I ATGCAT 1 cut(s) 3574
MroI TCCGGA 2 cut(s) 802, 940
MscI TGGCCA 1 cut(s) 1847
MslI CAYNNNNRTG 6 cut(s) 29, 170, 176, 3617, 3881, 4178
Msp20I TGGCCA 1 cut(s) 1847
MspA1I CMGCKG 5 cut(s) 11, 962, 1141, 2249, 4430
MspI CCGG 7 cut(s) 803, 941, 3346, 3778, 3844, 4777, 5000
MunI CAATTG 2 cut(s) 1041, 1980
Mva1269I GAATGC 3 cut(s) 3341, 4829, 5094
MvaI CCWGG 9 cut(s) 537, 756, 1116, 1489, 1622, 2472, 2873, 3374, 3881
MvnI CGCG 1 cut(s) 2359
NciI CCSGG 3 cut(s) 3347, 3778, 5001
NcoI CCATGG 1 cut(s) 4656
NdeI CATATG 2 cut(s) 3574, 3984
NheI GCTAGC 1 cut(s) 1396
NlaIV GGNNCC 7 cut(s) 748, 945, 1145, 2217, 3371, 3512, 5103
NmuCI GTSAC 8 cut(s) 94, 361, 385, 1347, 1813, 2284, 3596, 4373
NsiI ATGCAT 1 cut(s) 3574
PaeI GCATGC 1 cut(s) 1778
PagI TCATGA 2 cut(s) 3496, 4684
PasI CCCWGGG 1 cut(s) 1621
PciI ACATGT 2 cut(s) 2665, 4962
PciSI GCTCTTC 1 cut(s) 1419
PctI GAATGC 3 cut(s) 3341, 4829, 5094
PflMI CCANNNNNTGG 3 cut(s) 2028, 3457, 3647
PfoI TCCNGGA 2 cut(s) 2470, 4999
PleI GAGTC 9 cut(s) 433, 1074, 1967, 2008, 2281, 3170, 3430, 3858, 4897
PpsI GAGTC 9 cut(s) 433, 1074, 1967, 2008, 2281, 3170, 3430, 3858, 4897
Ppu21I YACGTR 1 cut(s) 3470
PpuMI RGGWCCY 1 cut(s) 2920
PscI ACATGT 2 cut(s) 2665, 4962
PshBI ATTAAT 3 cut(s) 66, 2370, 3945
PsiI TTATAA 1 cut(s) 4590
Psp124BI GAGCTC 2 cut(s) 2207, 3942
Psp5II RGGWCCY 1 cut(s) 2920
Psp6I CCWGG 9 cut(s) 535, 754, 1114, 1487, 1620, 2470, 2871, 3372, 3879
PspFI CCCAGC 3 cut(s) 376, 750, 1265
PspGI CCWGG 9 cut(s) 535, 754, 1114, 1487, 1620, 2470, 2871, 3372, 3879
PspN4I GGNNCC 7 cut(s) 748, 945, 1145, 2217, 3371, 3512, 5103
PspOMI GGGCCC 1 cut(s) 5101
PspPI GGNCC 7 cut(s) 2920, 3040, 3200, 3385, 3717, 5101, 5102
PspPPI RGGWCCY 1 cut(s) 2920
PstI CTGCAG 4 cut(s) 919, 1186, 1198, 4848
PstNI CAGNNNCTG 4 cut(s) 914, 3017, 3124, 4978
PsuI RGATCY 7 cut(s) 787, 943, 1435, 1615, 2050, 2215, 2782
PvuII CAGCTG 3 cut(s) 962, 2249, 4430
RseI CAYNNNNRTG 6 cut(s) 29, 170, 176, 3617, 3881, 4178
SacI GAGCTC 2 cut(s) 2207, 3942
SapI GCTCTTC 1 cut(s) 1419
Sau96I GGNCC 7 cut(s) 2920, 3040, 3200, 3385, 3717, 5101, 5102
ScaI AGTACT 2 cut(s) 1215, 1292
SchI GAGTC 9 cut(s) 434, 1074, 1968, 2008, 2281, 3171, 3431, 3858, 4897
SfcI CTRYAG 8 cut(s) 915, 1182, 1194, 3116, 3222, 4258, 4494, 4844
SinI GGWCC 2 cut(s) 2920, 3040
SmiMI CAYNNNNRTG 6 cut(s) 29, 170, 176, 3617, 3881, 4178
SmlI CTYRAG 7 cut(s) 255, 2036, 2200, 3746, 4541, 4559, 4793
SmoI CTYRAG 7 cut(s) 255, 2036, 2200, 3746, 4541, 4559, 4793
SphI GCATGC 1 cut(s) 1778
SspI AATATT 1 cut(s) 2768
SspMI CTAG 7 cut(s) 54, 902, 1397, 1430, 1811, 3737, 3965
SstI GAGCTC 2 cut(s) 2207, 3942
StyI CCWWGG 3 cut(s) 3381, 3546, 4656
TaiI ACGT 7 cut(s) 86, 604, 1354, 1482, 3472, 3979, 4573
TaqI TCGA 6 cut(s) 233, 931, 1556, 2571, 3589, 4719
TatI WGTACW 6 cut(s) 114, 1213, 1290, 2178, 2662, 3731
TauI GCSGC 3 cut(s) 8, 11, 142
TscAI CASTG 6 cut(s) 413, 1030, 2215, 3562, 3910, 4121
TseFI GTSAC 8 cut(s) 94, 361, 385, 1347, 1813, 2284, 3596, 4373
Tsp45I GTSAC 8 cut(s) 94, 361, 385, 1347, 1813, 2284, 3596, 4373
TspGWI ACGGA 2 cut(s) 1208, 4370
TspRI CASTG 6 cut(s) 413, 1030, 2215, 3562, 3910, 4121
Van91I CCANNNNNTGG 3 cut(s) 2028, 3457, 3647
VneI GTGCAC 2 cut(s) 3978, 4268
VpaK11BI GGWCC 2 cut(s) 2920, 3040
VspI ATTAAT 3 cut(s) 66, 2370, 3945
XapI RAATTY 8 cut(s) 1241, 1532, 1714, 2854, 3088, 3839, 3855, 4154
XbaI TCTAGA 1 cut(s) 901
XcmI CCANNNNNNNNNTGG 2 cut(s) 2884, 3454
XmiI GTMKAC 2 cut(s) 402, 3552
XspI CTAG 7 cut(s) 54, 902, 1397, 1430, 1811, 3737, 3965
ZrmI AGTACT 2 cut(s) 1215, 1292
Zsp2I ATGCAT 1 cut(s) 3574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.