RLG00000027035

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002740
Physical Location & Seq
Reverse (-)
7815007 .. 7824706
9700 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027035

Sequence Viewer

Length: 5076 bp
ATGGCGGCGGCTAACGCTCCGATCACCATGAAGGAGGCCTTAACGCTTCCGAGCGTTGGGATTAATCCGCAATTCATGACGTTTACACATGTTACTATGGAGTCCGATAAGTACATATGTGTTCGGGAAACAGCGCCGCAGAATAGTATTGTGATTATTGATATGAGTATGCCGAATCAGCCTTTGAGGAGGCCTATCACCGCCGACTCTGCACTTATGAATCCAAACTCCAAAATCCTTGCTTTGAAAGCTCAAGTCCAGGGAACTACTCAGGACCACCTTCAAATTTTTAACATTGAGATGAAAGCAAAACTGAAATCACACCTTATGCCTGAGCAGTTACATGTTTTCGGTTCTGAATGTATCCTCATTACTTTCTGTGTAGATTGTGAGTCTGAACCAGTAAAGGTATTTGAGCGAACAGCTAACCTGGCAAACAATCAGATCATAAATTACCGTTGTGATCCTTCTGAGAAGTGGTTGGTTCTGATTGGAATTGCTCCTGGTGCTCCTGAGAGGCCACAATTGGTTAAAGGGAACTTGCAACTCTTCTCGGTGGATCAGCAGCGCAGTCAAGCCCTTGAAGCGCATGCCGCATCCTTTGCCCAGTACAAGGTTCCAGGGAATGAGAATCCTTCCATTCTGATTTCCTTTGCCACTAAGACACTCAATTCTGGACAGATTACATCCAAGTTACATGTTATTGAGCTTGGTGCCCAGCCAGGGAAGCCATCTTTTACAAAGAAACAAGCAGATCTTTTCTTTCCTCCAGATTTTGCAGATGATTTCCCAGTTGCCATGCAGATGTCTCACAAGTACAGTTTGATTTATGTAATTACAAAGCTGGGGCTTCTATTTGTATACGACTTAGAGACAGCTAGTGCAGTTTACAGAAATAGAATTAGTCCAGATCCAATTTTCTTGACGACAGAAGCTTCATCAGTGGGAGGGTTTTATGCTGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACCATTAATGAGCAAACAATTGTGCCTTTTGTCAGTGGTCAGTTAAACAATTTGGAGCTTGCCGTCAATCTGGCAAAAAGAGGAAATCTTCCTGGTGCTGAGAATCTGGTTGTCCAGCGTTTCCAAGAACTGTTTGCCCAAACCAAGTACAAAGAAGCTGCCGAACTGGCTGCTGAGTCCCCGCAAGGAATTCTTCGTACACCGGATACAGTTGCTAAATTTCAGAGTGTTCCTGTCCAAGCTGGGCAAACACCCCCTTTGTTGCAATACTTTGGGACTCTCTTAACGAGAGGGAAGCTCAATGCCTTTGAGTCATTGGAATTGTCACGTCTTGTTGTGAACCAGAACAAGAAGAATCTTTTGGAAAACTGGTTGGCAGAGGACAAACTGGAATGCAGTGAGGAACTAGGAGATCTTGTGAAGACTGTGGATAATGATCTTGCTTTGAAAATATACATTAAAGCCAGAGCAACTCCAAAAGTTGTTGCAGCTTTTGCCGAGCGAAGGGAGTTTGACAAAATCCTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTATCTGTTCCTTTTGCAAACAATTCTCCGAGCAGACCCGCAGGGTGCTGTAAATTTTGCATTGATGATGTCCCAAATGGAGGGTGGTTGTCCTGTTGATTACAACACCATTACAGATCTCTTTCTTCAGAGGAATCTGATTCGTGAGGCAACCGCTTTTCTACTGGATGTTTTGAAGCCAAATCTGCCTGAACATGCCTTCCTGCAGACAAAGGTCCTCGAGATTAACTTGGTTACTTTTCCAAATGTGGCTGATGCCATTTTAGCCAATGGTATGTTCAGTCATTATGATCGTCCTCGAATTGCCCAGTTATGTGAAAAAGCTGGTCTTTATGTGCGAGCTTTGCAACACTACTCCGAGTTGCCTGATATTAAACGTGTCATAGTGAATACACATGCAATAGAGCCGCAGTCACTAGTAGAGTTTTTTGGTACCTTGTCAAAGGAATGGGCGTTGGAGTGTATGAAGGATCTCTTGCTGGTCAATTTGAGAGGCAACCTTCAGATAATTGTGCAGGTTGCTAAGGAGTACTCTGATCAGTTGGGTACTGATCAATGCATGAAGCTATTTGAGCAGTTCAAGTCATATGAAGGATTGTACTTCTTCCTCGGATCATTTTTGAGTTCAAGTGAGGATCCTGAAATCCACTTCAAGTACATTGAGGCAGCTGCCAAAACTGGACAAATTAAAGAGGTTGAGAGAGTTACTAGAGAATCAAATTTCTATGATGCAGAGAAAACAAAGAACTTTTTAATGGAAGCTAAGCTCCCTGATGCACGACCCCTTATCAATGTTTGCGACCGTTTTGGTTTTGTTCCAGATCTCACCCACTACTTGTACACAAACAATATGCTCCGTTACATTGAGGGTTACGTTCAAAAGGTGAATCCTTCAAATGCTCCTTTAGTTGTGGGACAGTTGCTAGATGATGAGTGCCCTGAAGATTTCATCAAAGGCTTGATCCTTTCTGTACGTTCATTGCTTCCAGTGGAGCCCCTTGTCGAGGAATGTGAGAAGAGGAATCGACTTCGTTTACTCACTCAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAGGATGTGCATGTCCACAATGCGTTGGGTAAAATCATCATTGATAGTGGAAACAATCCTGAACACTTCCTTACCACAAACCCATATTATGATTCTCGTGTTGTGGGTAAATATTGTGAGAAACGTGATCCTACTCTGGCTGTTGTTGCTTATCGACGTGGGCAATGCGATGATGAGCTTATCAATGTTACAAATAAAAACTCTCTGTTCAAATTACAAGCAAGATATGTCGTTGAGAGGATGGATGAGGATCTTTGGGCTAAAGTTCTTGACCCTGAGAATGAATTTAGAAGACAACTTATCGATCAAGTGGTTTCTACTGCCTTGCCTGAAAGCAAGAGCCCAGAACAAGTTTCTGCGGCTGTTAAGGCTTTCATGACTGCTGATTTGCCCCATGAATTAATTGAGCTTCTTGAAAAGATTGTGCTTCAAAACTCTGCCTTCAGTGGGAACTTTAATCTGCAAAATCTGCTCATTTTGACAGCCATCAAGGCAGATCCGTCCAGAGTTATGGATTATATTAATAGGCTAGACAATTTCGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCCCAACTGTATGAGGAAGCATTTGCAATTTTCAAGAAGTTCAACTTGAATGTCCAAGCTGTCAATGTCTTGCTGGATAATATCCGAAGCATTGATAGGGCTGTAGAATTTGCATTCCGTGTTGAAGAGGATGCTGTTTGGAGTCAGGTTGGAAAGGCTCAACTCCGGGAGGGACTTGTGAGTGATGCGATTGAGTCATTTATTCGGGCGGATGATGCCACACAATTCTTGGATGTTATTCGAGCTTCTGAAGATGCGGATGTTTACCATGACTTGGTGAGATACCTTCTGATGGTTAGGCAGAAGACAAAAGAGCCGAAGGTGGACAGTGAGCTCATTTATGCATATGCAAAGATTGATAGGCTGGCTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCCAATCTTCAAAATGTTGGTGATCGCCTATATGATGAGACCCTCTATGAGGCTGCAAAAATAATTTATGCTTTTATATCCAACTGGGCCAAGTTGGCTGTCACACTTGTCAAGCTCAAACAATTCCAAGGTGCTGTTGATGCAGCACGAAAAGCCAATAGTGCAAAGACATGGAAGGAAGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGCTTAGCTCAAATTTGTGGTCTCAACATCATTATACAGGTGGATGATTTGGAGGAGGTCAGTGAATTCTACCAGAATAGAGGGTGCTTCAATGAGCTAATATCTCTAATGGAAAGTGGTCTTGGCTTAGAACGTGCACACATGGGAATCTTTACTGAATTGGGGGTTCTGTATGCTAGATACCGTCCTGAGAAACTTATGGAGCACATCAAACTGTTCTCAACTCGTTTAAATATTCCCAAGCTCATTCGAGCTTGTGATGAGCAGCAGCATTGGAAGGAGCTAACCTATCTATATATTCAATATGATGAGTTTGATAATGCTGCAACAACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAATTCAAAGATGTGGCAGTTAAAGTTGCAAATGTGGAGCTATATTATAAGGCTGTGCACTTTTACTTGCAAGAGCATCCAGATCTCATTAATGATATTCTAAATGTGCTTGCTCTCCGTGTAGATCATACACGTGTGGTGGACATTATGCGAAAGGCTGGGCACCTACTCCTTGTGAAGCCATACATGGTTGCTGTTCAGAGCAACAATGTCTCAGCTGTGAATGAGGCTTTGAATGAGATATATGTTGAAGAGGAAGACTATGAAAGATTGCGTGAATCAATTGATTTACATGACAGCTTTGATCAAATAGGTCTTGCTCAGAAGATTGAAAAACACGAGCTTCTGGAAATGAGGCGTGTTGCTGCTTATATTTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGCATTATCAAAGAAAGACAAACTTTACAAAGATGCCATGGAGACAGCTTCACAGTCTGGTGACCGCGAACTTGCGGAGGAATTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCATCATGCCTCTTCGTCTGTTACGATTTGATTAGGCCGGATACTGCCCTTGAACTTGCTTGGATGAACAACATGATAGACTTTGCCTTCCCATATCTGTTGCAGTTCATCCGTGAATACACCGGTAAAGTCGATGAATTGGTGAGGGACAGAATTGAAGCCCAGAATGAGGTCAAGGAGAAGGAGAAGGAAGAGAAAGAAGTTATAGCACAGCAGAACATGTATGCACAGTTGTTGCCTCTTGCTTTGCCTGCTCCACCAATGCCTGGTATGGGAGGAGGGTATGGTCCTCCGCCTCCAATGGGAGGAATGGGGATGCCTCCGATGCCACCTTATGGTATGCCACCAATGGGCAGTAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1692

Amino Acids

191.63

Weight (kDa)

5.22

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 4.7e-06 Clathrin propeller repeat
Clathrin-link PF09268 334 - 357 9.2e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 359 - 424 4e-28 Clathrin-H-link
Clathrin PF00637 547 - 682 7.8e-25 Region in Clathrin and VPS
Clathrin PF00637 691 - 831 7.4e-27 Region in Clathrin and VPS
Clathrin PF00637 840 - 974 1.6e-32 Region in Clathrin and VPS
Clathrin PF00637 983 - 1121 4e-32 Region in Clathrin and VPS
Clathrin PF00637 1134 - 1271 1.1e-30 Region in Clathrin and VPS
Clathrin PF00637 1280 - 1422 2.2e-27 Region in Clathrin and VPS
Clathrin PF00637 1428 - 1569 3.6e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4227
Acc36I ACCTGC 3 cut(s) 1519, 2053, 3178
Acc65I GGTACC 1 cut(s) 1979
AccB1I GGYRCC 3 cut(s) 713, 1979, 4341
AccB7I CCANNNNNTGG 3 cut(s) 3469, 4976, 5045
AccI GTMKAC 1 cut(s) 861
AccII CGCG 1 cut(s) 4653
AcsI RAATTY 9 cut(s) 285, 1179, 1208, 1600, 2266, 2906, 3302, 3822, 3875
AcuI CTGAAG 6 cut(s) 1658, 2033, 2508, 3049, 3465, 4182
AcvI CACGTG 1 cut(s) 4313
AflIII ACRYGT 7 cut(s) 88, 343, 697, 1924, 4310, 4312, 4929
AgeI ACCGGT 1 cut(s) 4832
AhlI ACTAGT 1 cut(s) 1963
AjiI CACGTC 2 cut(s) 1319, 2780
AjuI GAANNNNNNNTTGG 6 cut(s) 1334, 1366, 1808, 1840, 3915, 3947
AloI GAACNNNNNNTCC 2 cut(s) 1731, 1763
Alw21I GWGCWC 5 cut(s) 511, 3531, 3949, 4017, 4239
Alw26I GTCTC 6 cut(s) 813, 866, 3629, 3836, 4396, 4622
Alw44I GTGCAC 2 cut(s) 3945, 4235
Ama87I CYCGRG 1 cut(s) 1766
AoxI GGCC 6 cut(s) 36, 191, 518, 973, 3684, 4745
ApaLI GTGCAC 2 cut(s) 3945, 4235
ApoI RAATTY 9 cut(s) 285, 1179, 1208, 1600, 2266, 2906, 3302, 3822, 3875
ArsI GACNNNNNNTTYG 4 cut(s) 2992, 3024, 3814, 3846
AseI ATTAAT 5 cut(s) 63, 996, 3023, 3144, 4269
AsiGI ACCGGT 1 cut(s) 4832
Asp718I GGTACC 1 cut(s) 1979
AspLEI GCGC 3 cut(s) 136, 570, 589
AspS9I GGNCC 6 cut(s) 274, 973, 1762, 3167, 3684, 4997
AsuC2I CCSGG 1 cut(s) 3362
AsuHPI GGTGA 8 cut(s) 16, 190, 2365, 2443, 3484, 3629, 4658, 4864
AvaI CYCGRG 1 cut(s) 1766
AvaII GGWCC 4 cut(s) 274, 1762, 3167, 4997
BaeGI GKGCMC 5 cut(s) 718, 2486, 3949, 4239, 4344
BamHI GGATCC 1 cut(s) 2182
BanI GGYRCC 3 cut(s) 713, 1979, 4341
BanII GRGCYC 3 cut(s) 2544, 2966, 3531
BauI CACGAG 2 cut(s) 2718, 4517
BbrPI CACGTG 1 cut(s) 4313
BbsI GAAGAC 4 cut(s) 1418, 2920, 3506, 4443
Bbv12I GWGCWC 5 cut(s) 511, 3531, 3949, 4017, 4239
BbvCI CCTCAGC 1 cut(s) 3801
BccI CCATC 6 cut(s) 739, 2857, 3116, 3158, 3481, 4151
BceAI ACGGC 1 cut(s) 1037
BcgI CGANNNNNNTGC 4 cut(s) 1142, 1176, 3152, 3186
BciVI GTATCC 3 cut(s) 374, 1189, 4744
BclI TGATCA 3 cut(s) 2083, 2098, 4483
BcnI CCSGG 1 cut(s) 3362
BcoDI GTCTC 6 cut(s) 813, 866, 3629, 3836, 4396, 4622
BcuI ACTAGT 1 cut(s) 1963
BfaI CTAG 7 cut(s) 879, 1397, 1964, 2256, 2471, 3152, 3987
BfmI CTRYAG 3 cut(s) 1751, 3171, 3297
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 3 cut(s) 1519, 2053, 3178
BfuI GTATCC 3 cut(s) 374, 1189, 4744
BglI GCCNNNNNGGC 3 cut(s) 1157, 3113, 3692
BglII AGATCT 5 cut(s) 754, 1402, 1663, 2368, 4261
BlpI GCTNAGC 2 cut(s) 2310, 3814
BmcAI AGTACT 1 cut(s) 2078
Bme18I GGWCC 4 cut(s) 274, 1762, 3167, 4997
BmeT110I CYCGRG 1 cut(s) 1766
BmgBI CACGTC 2 cut(s) 1319, 2780
BmgT120I GGNCC 6 cut(s) 274, 973, 1762, 3167, 3684, 4997
BmiI GGNNCC 7 cut(s) 618, 715, 974, 1981, 2184, 2541, 4343
BmrI ACTGGG 4 cut(s) 601, 785, 1849, 3691
BmuI ACTGGG 4 cut(s) 601, 785, 1849, 3691
BoxI GACNNNNGTC 1 cut(s) 1760
BpiI GAAGAC 4 cut(s) 1418, 2920, 3506, 4443
BplI GAGNNNNNCTC 2 cut(s) 1272, 1304
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 3 cut(s) 333, 2070, 3801
Bpu1102I GCTNAGC 2 cut(s) 2310, 3814
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 3362
Bsa29I ATCGAT 1 cut(s) 2925
BsaAI YACGTR 1 cut(s) 4313
BsaBI GATNNNNATC 2 cut(s) 1425, 2871
BsaI GGTCTC 2 cut(s) 3629, 3836
BsaJI CCNNGG 7 cut(s) 259, 620, 722, 2155, 3724, 4167, 4623
BsaWI WCCGGW 2 cut(s) 1192, 4832
BsaXI ACNNNNNCTCC 2 cut(s) 1392, 1422
Bse118I RCCGGY 1 cut(s) 4832
Bse3DI GCAATG 3 cut(s) 2525, 2792, 4584
Bse8I GATNNNNATC 2 cut(s) 1425, 2871
BseCI ATCGAT 1 cut(s) 2925
BseDI CCNNGG 7 cut(s) 259, 620, 722, 2155, 3724, 4167, 4623
BseJI GATNNNNATC 2 cut(s) 1425, 2871
BseMI GCAATG 3 cut(s) 2525, 2792, 4584
BseRI GAGGAG 4 cut(s) 202, 3818, 3878, 5001
BseSI GKGCMC 5 cut(s) 718, 2486, 3949, 4239, 4344
BseYI CCCAGC 4 cut(s) 717, 844, 1232, 4337
BsgI GTGCAG 3 cut(s) 195, 903, 2081
Bsh1236I CGCG 1 cut(s) 4653
Bsh1285I CGRYCG 1 cut(s) 2350
BshFI GGCC 6 cut(s) 38, 193, 520, 975, 3686, 4747
BshNI GGYRCC 3 cut(s) 713, 1979, 4341
BshTI ACCGGT 1 cut(s) 4832
BshVI ATCGAT 1 cut(s) 2925
BsiEI CGRYCG 1 cut(s) 2350
BsiHKAI GWGCWC 5 cut(s) 511, 3531, 3949, 4017, 4239
BsiHKCI CYCGRG 1 cut(s) 1766
BsiSI CCGG 4 cut(s) 1193, 3361, 4748, 4833
BslFI GGGAC 6 cut(s) 1153, 1279, 1603, 2475, 3381, 4871
BsmAI GTCTC 6 cut(s) 813, 866, 3629, 3836, 4396, 4622
BsmFI GGGAC 6 cut(s) 1153, 1279, 1603, 2475, 3381, 4871
BsmI GAATGC 3 cut(s) 1388, 3308, 4709
BsnI GGCC 6 cut(s) 38, 193, 520, 975, 3686, 4747
Bso31I GGTCTC 2 cut(s) 3629, 3836
BsoBI CYCGRG 1 cut(s) 1766
Bsp1407I TGTACA 1 cut(s) 2385
Bsp1720I GCTNAGC 2 cut(s) 2310, 3814
Bsp19I CCATGG 1 cut(s) 4623
BspANI GGCC 6 cut(s) 38, 193, 520, 975, 3686, 4747
BspDI ATCGAT 1 cut(s) 2925
BspFNI CGCG 1 cut(s) 4653
BspHI TCATGA 3 cut(s) 75, 2997, 4146
BspLI GGNNCC 7 cut(s) 618, 715, 974, 1981, 2184, 2541, 4343
BspMAI CTGCAG 2 cut(s) 1755, 3175
BspMI ACCTGC 3 cut(s) 1519, 2053, 3178
BspT107I GGYRCC 3 cut(s) 713, 1979, 4341
BspTNI GGTCTC 2 cut(s) 3629, 3836
BsrDI GCAATG 3 cut(s) 2525, 2792, 4584
BsrFI RCCGGY 1 cut(s) 4832
BsrGI TGTACA 1 cut(s) 2385
BssAI RCCGGY 1 cut(s) 4832
BssECI CCNNGG 7 cut(s) 259, 620, 722, 2155, 3724, 4167, 4623
BssNAI GTATAC 1 cut(s) 862
BssSI CACGAG 2 cut(s) 2718, 4517
BssT1I CCWWGG 2 cut(s) 3724, 4623
Bst1107I GTATAC 1 cut(s) 862
Bst2BI CACGAG 2 cut(s) 2718, 4517
Bst6I CTCTTC 7 cut(s) 554, 2558, 3315, 3567, 4425, 4727, 4896
BstAPI GCANNNNNTGC 3 cut(s) 602, 1484, 3091
BstAUI TGTACA 1 cut(s) 2385
BstBAI YACGTR 1 cut(s) 4313
BstC8I GCNNGC 6 cut(s) 591, 1050, 1887, 3561, 4290, 4962
BstDSI CCRYGG 1 cut(s) 4623
BstEII GGTNACC 1 cut(s) 4646
BstENI CCTNNNNNAGG 2 cut(s) 2549, 3644
BstFNI CGCG 1 cut(s) 4653
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 3 cut(s) 136, 570, 589
BstMAI GTCTC 6 cut(s) 813, 866, 3629, 3836, 4396, 4622
BstMCI CGRYCG 1 cut(s) 2350
BstNSI RCATGY 9 cut(s) 92, 347, 593, 701, 1745, 1946, 2636, 4180, 4933
BstPAI GACNNNNGTC 1 cut(s) 1760
BstPI GGTNACC 1 cut(s) 4646
BstSFI CTRYAG 3 cut(s) 1751, 3171, 3297
BstSLI GKGCMC 5 cut(s) 718, 2486, 3949, 4239, 4344
BstUI CGCG 1 cut(s) 4653
BstV2I GAAGAC 4 cut(s) 1418, 2920, 3506, 4443
BstXI CCANNNNNNTGG 1 cut(s) 3133
BstZ17I GTATAC 1 cut(s) 862
Bsu15I ATCGAT 1 cut(s) 2925
BsuI GTATCC 3 cut(s) 374, 1189, 4744
BsuRI GGCC 6 cut(s) 38, 193, 520, 975, 3686, 4747
BsuTUI ATCGAT 1 cut(s) 2925
BtgI CCRYGG 1 cut(s) 4623
BtgZI GCGATG 1 cut(s) 2805
BtrI CACGTC 2 cut(s) 1319, 2780
BtsI GCAGTG 1 cut(s) 1393
BtsIMutI CAGTG 7 cut(s) 948, 1030, 1393, 2541, 3073, 3529, 3877
BveI ACCTGC 3 cut(s) 1519, 2053, 3178
Cac8I GCNNGC 6 cut(s) 591, 1050, 1887, 3561, 4290, 4962
CciI TCATGA 3 cut(s) 75, 2997, 4146
CfoI GCGC 3 cut(s) 136, 570, 589
Cfr10I RCCGGY 1 cut(s) 4832
Cfr13I GGNCC 6 cut(s) 274, 973, 1762, 3167, 3684, 4997
ClaI ATCGAT 1 cut(s) 2925
CspAI ACCGGT 1 cut(s) 4832
DraI TTTAAA 1 cut(s) 4041
Eam1104I CTCTTC 7 cut(s) 554, 2558, 3315, 3567, 4425, 4727, 4896
EarI CTCTTC 7 cut(s) 554, 2558, 3315, 3567, 4425, 4727, 4896
EciI GGCGGA 2 cut(s) 3419, 4992
Ecl136II GAGCTC 1 cut(s) 3529
Eco130I CCWWGG 2 cut(s) 3724, 4623
Eco147I AGGCCT 2 cut(s) 38, 193
Eco24I GRGCYC 3 cut(s) 2544, 2966, 3531
Eco31I GGTCTC 2 cut(s) 3629, 3836
Eco47I GGWCC 4 cut(s) 274, 1762, 3167, 4997
Eco53kI GAGCTC 1 cut(s) 3529
Eco57I CTGAAG 6 cut(s) 1658, 2033, 2508, 3049, 3465, 4182
Eco72I CACGTG 1 cut(s) 4313
Eco88I CYCGRG 1 cut(s) 1766
Eco91I GGTNACC 1 cut(s) 4646
EcoICRI GAGCTC 1 cut(s) 3529
EcoNI CCTNNNNNAGG 2 cut(s) 2549, 3644
EcoO109I RGGNCCY 1 cut(s) 1762
EcoO65I GGTNACC 1 cut(s) 4646
EcoRI GAATTC 2 cut(s) 1179, 3875
EcoT14I CCWWGG 2 cut(s) 3724, 4623
EcoT22I ATGCAT 2 cut(s) 2108, 3541
EcoT38I GRGCYC 3 cut(s) 2544, 2966, 3531
ErhI CCWWGG 2 cut(s) 3724, 4623
FaqI GGGAC 6 cut(s) 1153, 1279, 1603, 2475, 3381, 4871
FauI CCCGC 3 cut(s) 963, 1179, 1593
FauNDI CATATG 3 cut(s) 116, 2134, 3541
FbaI TGATCA 3 cut(s) 2083, 2098, 4483
FblI GTMKAC 1 cut(s) 861
FriOI GRGCYC 3 cut(s) 2544, 2966, 3531
FspBI CTAG 7 cut(s) 879, 1397, 1964, 2256, 2471, 3152, 3987
GlaI GCGC 3 cut(s) 135, 569, 588
GsaI CCCAGC 4 cut(s) 721, 848, 1236, 4341
GsuI CTGGAG 1 cut(s) 753
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 6 cut(s) 38, 193, 520, 975, 3686, 4747
HapII CCGG 4 cut(s) 1193, 3361, 4748, 4833
HhaI GCGC 3 cut(s) 136, 570, 589
Hin6I GCGC 3 cut(s) 134, 568, 587
HinP1I GCGC 3 cut(s) 134, 568, 587
HindIII AAGCTT 1 cut(s) 933
HpaII CCGG 4 cut(s) 1193, 3361, 4748, 4833
HphI GGTGA 8 cut(s) 16, 190, 2365, 2443, 3484, 3629, 4658, 4864
Hpy99I CGWCG 1 cut(s) 2781
HpyCH4IV ACGT 9 cut(s) 80, 1318, 1924, 2421, 2521, 2746, 2779, 3943, 4312
HpySE526I ACGT 9 cut(s) 80, 1318, 1924, 2421, 2521, 2746, 2779, 3943, 4312
HspAI GCGC 3 cut(s) 134, 568, 587
KpnI GGTACC 1 cut(s) 1983
Ksp22I TGATCA 3 cut(s) 2083, 2098, 4483
MaeI CTAG 7 cut(s) 879, 1397, 1964, 2256, 2471, 3152, 3987
MaeII ACGT 9 cut(s) 80, 1318, 1924, 2421, 2521, 2746, 2779, 3943, 4312
MfeI CAATTG 3 cut(s) 524, 1008, 4461
MlyI GAGTC 8 cut(s) 110, 200, 401, 1175, 1261, 1310, 3346, 3398
MmeI TCCRAC 3 cut(s) 1983, 3325, 3702
Mph1103I ATGCAT 2 cut(s) 2108, 3541
MslI CAYNNNNRTG 4 cut(s) 299, 803, 3584, 4311
MspA1I CMGCKG 2 cut(s) 2216, 4397
MspI CCGG 4 cut(s) 1193, 3361, 4748, 4833
MunI CAATTG 3 cut(s) 524, 1008, 4461
Mva1269I GAATGC 3 cut(s) 1388, 3308, 4709
MvnI CGCG 1 cut(s) 4653
NciI CCSGG 1 cut(s) 3362
NcoI CCATGG 1 cut(s) 4623
NdeI CATATG 3 cut(s) 116, 2134, 3541
NlaIV GGNNCC 7 cut(s) 618, 715, 974, 1981, 2184, 2541, 4343
NmeAIII GCCGAG 1 cut(s) 1513
NmuCI GTSAC 4 cut(s) 1314, 1959, 3697, 4646
NsiI ATGCAT 2 cut(s) 2108, 3541
NspI RCATGY 9 cut(s) 92, 347, 593, 701, 1745, 1946, 2636, 4180, 4933
PaeI GCATGC 1 cut(s) 593
PaeR7I CTCGAG 1 cut(s) 1766
PagI TCATGA 3 cut(s) 75, 2997, 4146
PceI AGGCCT 2 cut(s) 38, 193
PciI ACATGT 4 cut(s) 88, 343, 697, 4929
PcsI WCGNNNNNNNCGW 1 cut(s) 4731
PctI GAATGC 3 cut(s) 1388, 3308, 4709
PflMI CCANNNNNTGG 3 cut(s) 3469, 4976, 5045
PfoI TCCNGGA 1 cut(s) 3360
PinAI ACCGGT 1 cut(s) 4832
PleI GAGTC 8 cut(s) 109, 200, 400, 1174, 1261, 1309, 3345, 3397
PmaCI CACGTG 1 cut(s) 4313
PmlI CACGTG 1 cut(s) 4313
PpsI GAGTC 8 cut(s) 109, 200, 400, 1174, 1261, 1309, 3345, 3397
Ppu21I YACGTR 1 cut(s) 4313
PpuMI RGGWCCY 1 cut(s) 1762
PscI ACATGT 4 cut(s) 88, 343, 697, 4929
PshAI GACNNNNGTC 1 cut(s) 1760
PshBI ATTAAT 5 cut(s) 63, 996, 3023, 3144, 4269
PsiI TTATAA 1 cut(s) 4227
Psp124BI GAGCTC 1 cut(s) 3531
Psp5II RGGWCCY 1 cut(s) 1762
PspCI CACGTG 1 cut(s) 4313
PspEI GGTNACC 1 cut(s) 4646
PspFI CCCAGC 4 cut(s) 717, 844, 1232, 4337
PspN4I GGNNCC 7 cut(s) 618, 715, 974, 1981, 2184, 2541, 4343
PspPI GGNCC 6 cut(s) 274, 973, 1762, 3167, 3684, 4997
PspPPI RGGWCCY 1 cut(s) 1762
PstI CTGCAG 2 cut(s) 1755, 3175
PvuII CAGCTG 2 cut(s) 2216, 4397
RseI CAYNNNNRTG 4 cut(s) 299, 803, 3584, 4311
SacI GAGCTC 1 cut(s) 3531
Sau96I GGNCC 6 cut(s) 274, 973, 1762, 3167, 3684, 4997
ScaI AGTACT 1 cut(s) 2078
SchI GAGTC 8 cut(s) 110, 200, 401, 1175, 1261, 1310, 3346, 3398
SfcI CTRYAG 3 cut(s) 1751, 3171, 3297
Sfr274I CTCGAG 1 cut(s) 1766
SinI GGWCC 4 cut(s) 274, 1762, 3167, 4997
SlaI CTCGAG 1 cut(s) 1766
SmiMI CAYNNNNRTG 4 cut(s) 299, 803, 3584, 4311
SmlI CTYRAG 2 cut(s) 252, 1766
SmoI CTYRAG 2 cut(s) 252, 1766
SpeI ACTAGT 1 cut(s) 1963
SphI GCATGC 1 cut(s) 593
SseBI AGGCCT 2 cut(s) 38, 193
SspI AATATT 2 cut(s) 2735, 4045
SspMI CTAG 7 cut(s) 879, 1397, 1964, 2256, 2471, 3152, 3987
SstI GAGCTC 1 cut(s) 3531
StuI AGGCCT 2 cut(s) 38, 193
StyI CCWWGG 2 cut(s) 3724, 4623
TaiI ACGT 9 cut(s) 83, 1321, 1927, 2424, 2524, 2749, 2782, 3946, 4315
TatI WGTACW 8 cut(s) 111, 609, 816, 1137, 2076, 2145, 2202, 2385
TauI GCSGC 6 cut(s) 8, 11, 139, 596, 1957, 2984
TscAI CASTG 7 cut(s) 948, 1030, 1393, 2541, 3073, 3529, 3877
TseFI GTSAC 4 cut(s) 1314, 1959, 3697, 4646
Tsp45I GTSAC 4 cut(s) 1314, 1959, 3697, 4646
TspGWI ACGGA 5 cut(s) 2393, 3111, 3302, 4286, 4811
TspRI CASTG 7 cut(s) 948, 1030, 1393, 2541, 3073, 3529, 3877
Van91I CCANNNNNTGG 3 cut(s) 3469, 4976, 5045
VneI GTGCAC 2 cut(s) 3945, 4235
VpaK11BI GGWCC 4 cut(s) 274, 1762, 3167, 4997
VspI ATTAAT 5 cut(s) 63, 996, 3023, 3144, 4269
XagI CCTNNNNNAGG 2 cut(s) 2549, 3644
XapI RAATTY 9 cut(s) 285, 1179, 1208, 1600, 2266, 2906, 3302, 3822, 3875
XceI RCATGY 9 cut(s) 92, 347, 593, 701, 1745, 1946, 2636, 4180, 4933
XcmI CCANNNNNNNNNTGG 2 cut(s) 1628, 3421
XhoI CTCGAG 1 cut(s) 1766
XmiI GTMKAC 1 cut(s) 861
XspI CTAG 7 cut(s) 879, 1397, 1964, 2256, 2471, 3152, 3987
ZrmI AGTACT 1 cut(s) 2078
Zsp2I ATGCAT 2 cut(s) 2108, 3541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.