MD03G1065300.v1.1

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002740
Physical Location & Seq
Reverse (-)
5198913 .. 5209097
10185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1065300.v1.1.491

Sequence Viewer

Length: 5100 bp
ATGGCCGCCGCTAACGCCCCCATGACCATGAAAGAGGCCCTCACGCTGCCTAGTATTGGGATCAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGTGTTAGGGAGACATCACCGCAGAATAGCGTCGTTATCATCGATATGAACATGCCCATGCAGCCGTTGCGTCGACCCATTACCGCCGACTCGGCTTTGATGAATCCCAATTCCAGAATTCTTGCTCTAAAAGCTCAAGTCCAAGGAACTGCTCAGGACCATTTACAAATCTTTAACATTGAAATGAAAGCAAAGATGAAATCACATCAAATGCCTGAGCAGGTTGTCTTTTGGAAGTGGATCACTCCAAAGATGCTAGGTCTAGTCACTCAGACCTCAGTATATCATTGGTTAATTGAAGGTGATTCCGAGCCTACAAAGGTCTTTGAGCGGACAGCTAATTTGGCAAACAATCAAATAATCAACTACCGTTGTGATCCTTCGGAAAAGTGGTTGGTCTTAATTGGAATTGCTCCTGGTTCAGCAGAGAGGCCACAACTTGTCAAAGGAAACATGCAGCTTTTTTCTGTGGAACAGAAACGTAGTCAGGCTCTTGAAGCACATGCTGCATCGTTTGCCACATTTAAAGTTCCAGGGAATGAGCATCCTTCAATTCTTATTTGTTTTGCCTCAAAGTCCTCCAATGCTGGACAGGTTACATCAAAGATGCATGTAATTGAGCTTGGTGCCCAGCCCGGAAAACCATCATATACTAAGAAGCAAGCAGATTTATTCTTTCCTCCTGATTTTGCTGATGACTTTCCAGTATCAATGCAGATATCGGAAAAGTATGGGTTGATATATGTTGTCACAAAGCTTGGACTTTTATTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCAGATCCTATATTTTTGACAGCTGAAGCTTCAACTGTTGGGGGTTTTTATGCAATCAATAGGAGAGGCCAGGTCTTACTTGCCACTATAAATGAACAAACAATTGTACCATTTGTTAGTGGTCAGTTAAACAATTTGGGGCTTGCAGTGAGTCTTGCGAAAAGAGGAAACCTTCCTGGTGCTGAGGACCTGGTTGTCCAGCGGTTCCAAGAGTTGTTTTCTCAGACAAAGTATAAGGAGGCTGCAGAGCTTGCCGCGGAATCTCCAATGGGAATACTCCGTACTCCTGATACTGTTGCTAAATTTCAGAGTGTTCCTGTGCAAGCTGGGCAAACCCCACCTCTATTGCAGTATTTTGGAACTCTTTTAACTAAGGGAAAACTCAATGCTTATGAATCATTAGAACTGTCACGTTTAGTTGTAAATCAGAACAAAAAGAATCTTTTGGAGAATTGGCTAGCAGAGGACAAGCTAGAATGTAGCGAAGAGCTTGGAGATCTTGTGAAGACAGTGGATAATGACCTTGCTTTGAAAATATACATCAAAGCTCGAGCAACGCCCAAAGTTGTTGCAGCATTTGCTGAGAGAAGGGAATTTGACAAGATTTTGATATACTCAAAGCAGGTTGGATATACACCTGATTATTTGTTCCTTCTCCAAACAATTTTGCGGGCAGATCCCCAGGGGGCTGTTAATTTTGCTCTAATGATGTCTCAAATGGAGGGAGGTTGCCCCATTGACTACAATACCATCACCGATCTGTTCCTTCAGAGGAACTTGATCCGTGAAGCAACAGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCTGAGCATGGATTCCTTCAGACAAAGGTTTTGGAAATCAATCTTGTGACTTTTCCAAATGTTGCGGATGCTATATTGGCCAATGGTATGTTCAGCTATTATGATCGCCCACGCATTGGTCAACTTTGTGAAAAAGCTGGTTTGTATTTGCGTGCCCTACAGCACTACACTGAATTGCCTGATATCAAACGTGTCATCGTGAACACACATGCAATTGACCCTCAGGCTCTTGTCGAGTTCTTTGGAACTCTCTCTGTAGAATGGGCTCTTGAGTGCATGAAAGATCTTCTGCTGGTCAATCTTAGGGGCAATCTTCAGATTATTGTACAGACCGCCAAAGAGTATTGCGAGCAACTGGGTGTAGATAAGTGTATAAAATTGTTTGAGCAGTTCAAGTCCTATGAGGGGCTGTACTTCTTCCTAGGCGCATATTTGAGCTCCAGCGAGGATCCTGATATTCATTTCAAGTACATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGTGTGACACGGGAATCAAATTTTTATGACCCTGAAAAGGCTAAGAACTTTTTAATGGAAGCAAAGCTTCCAGACGCACGTCCTCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCAGATCTCACCCATTATCTGTATACGAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTAGGGCAGCTACTGGATGATGAATGTCCTGAAGATTTCATTAAGGGTCTTATTCTATCAGTTCGTTCTCTTCTACCGGTTGAGCCCCTTGTCGAGGAATGTGAAAAGAGGAATCGTCTTCGCTTGCTCACTCAATTTTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGACGTACATGTCCACAATGCTCTGGGTAAAATCATCATAGATAGCGGCAACAATCCTGAGCATTTTCTCACAACTAACCCATACTATGATTCTCGCGTTGTGGGTAAATATTGTGAGAAAAGGGATCCTACCCTTGCTGTTGTTGCATACCGTAGAGGGCAATGTGATGATGAGCTCATTAATGTTACAAATAAAAATTCCTTGTTCAAACTCCAGGCCAGATATGTCGTGGAAAGAATGGATGATGACCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAATATAAAAGGCAACTAATCGATCAAGTTGTATCTACTGCCTTGCCTGAAAGCAAGAGTCCTGAGCAAGTTTCGGCAACTGTTAAAGCATTTATGACTGCGGACCTTCCACACGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTGCAAAATTCTGCTTTTAGTGGGAACTTCAATCTGCAGAATCTGTTAATTCTGACTGCCATCAAAACTGATCCATCCAGAGTCATGGACTATATCAATAGATTAGACAACTTTGATGGACCCGCAGTTGGAGTAATGGCTGTAGAAGCTCAATTGTATGAAGAAGCATTTGCAATCTTCAAGAAGTTCAATTTGAATGTTGATGCTGTGAATGTCCTGTTGGATAACATTCAGAGCATTGACCGAGCTGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTGGCCAAGGCCCAATTGCGAGAGGGATTAGTGAGTGATGCCATCGAATCATTCATCCGTGCAGATGATGCCACCCACTTTTTGGATGTCATACGTGCTTCTGAGGATGCAGATGTATATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGACCAAGGAGCCGAAGGTCGACAGTGAACTTATTTATGCGTATGCTAAGATTGATCGGCTTGGTGATATTGAAGAATTCATTCTTATGCCAAATGTTGCCAATCTCCAAAATGTTGGGGATCGCTTGTTTGATGAGGCCCTATACGAAGCAGCCAAGATCATATTTGCATTCATTTCTAACTGGGGTAAACTAGCATGTACTCTTGTGAAGCTAAATCAGTTTCAAGGTGCCGTTGATGCAGCTCGGAAAGCTAACAGCTCAAAAACGTGGAAGTTTGTTTGCTTTGCATGCGTTGATGCTGAGGAATTCCGCTTAGCTCAGATTTGTGGACTCAACATCATCATTCAGGTGGATGATTTGGAAGAGGTCTGTGAGTATTACCAGAACAGGGGATGCTTTAATGAGCTCATTAGTCTCATGGAGAGTGGGCTAGGGTTGGAGCGTGCACATATGGGCATCTTTACGGAATTAGGAGTTCTATATGCAAGATACCGTCCTGAGAAGCTTATGGAACACATTAAACTGTTCTCTACCCGTCTGAACATTCCCAAGCTTATACGAGCTTGTGATGAACAGCAGCACTGGAAAGAATTAACATACTTATATATCCAATATGATGAGTTTGATAATGCTGCAACTACCATAATGAACCACTCCCCAGAAGCATGGGACCACATGCAATTCAAGGATGTTATTGTCAAAGTTGCAAGTGTCGAACTCTACTATAGGGCTGTGCACTTCTACTTAGAAGAACATCCTGATGTTATTAATGATGTTCTTAATGTGATTGCACTTCGTGTCGACCACACCAGAGTGGTGGATATAATGAGAAAGGCTGGTCACATCTGTCTCATAAAGCCATACATGGTTGCAGTTCAGAGCAACAATGTAACTGCTGTAAATGAAGCTCTAAATGAAATATACATAGAGGAGGAAGACTATGACAGGCTTCGTGAATCAATAGATTTACACGACAGCTTTGATCAGATCGGTCTTGCTCAAAAGCTTGAGAAACATGAGCTTTTGGAGATGAGACGTGTTGCTGCATACATTTACAAGAAAGCAGGTAGATGGAAGCAATCCATTGCCTTGTCAAAGAAAGACAATCATTACAAAGATTGCATGGAGACATGCTCACAGTCTGGTGATCATGAACTTTCGGAGGAACTGCTTATCTATTTCATTGAGCAGGGAAAGAAAGAGTGTTTTGCTGCATGCCTATTTGTTTGTTATGATATGATCCGGCCAGATGTTGCCCTCGAGCTTGCGTGGATGAACAATATAATTGATTTTGCCTTACCATACCTTCTGCAGTTTATTCGAGAGTACACAGGAAAGGTTGATGAACTTATTAAAGACAAAATCGAAGCTCAAAACGAGGTGAAAGCCAAAGAGAAAGAGGAGAAGGAATTGGTTGCCCAGCAGAATATGTATGCTCAGTTGCTGCCTGCCTTGCCTGCACCACCGGGAATGGGAGGTTATGCTCCACCACCAATGCCGCCAATGGGAGGGATGGGGATGCCGCCAATGCCACCATTTGGAATGCCGCAAATGGGCCCCTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1700

Amino Acids

192.64

Weight (kDa)

5.24

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 56 1.4e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 7.5e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 1.8e-27 Clathrin-H-link
Clathrin PF00637 557 - 692 1.1e-24 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 8.4e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 1e-32 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 1.1e-32 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 1.7e-28 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1431 2e-27 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 4.6e-30 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1130, 2663
Acc36I ACCTGC 3 cut(s) 328, 1549, 4586
Acc65I GGTACC 1 cut(s) 3507
AccB1I GGYRCC 3 cut(s) 743, 3507, 3758
AccB7I CCANNNNNTGG 3 cut(s) 1880, 3454, 5069
AccBSI CCGCTC 1 cut(s) 448
AccI GTMKAC 4 cut(s) 190, 2417, 3549, 4332
AccII CGCG 2 cut(s) 1193, 2751
AcoI YGGCCR 4 cut(s) 3, 1842, 3375, 4775
AcsI RAATTY 8 cut(s) 234, 1238, 1529, 2296, 2851, 3085, 3605, 3836
AcuI CTGAAG 6 cut(s) 981, 1688, 1766, 1772, 2063, 2538
AdeI CACNNNGTG 1 cut(s) 4328
AflIII ACRYGT 3 cut(s) 1954, 2662, 4567
AgeI ACCGGT 1 cut(s) 2563
AjiI CACGTC 2 cut(s) 2357, 4568
AjnI CCWGG 9 cut(s) 532, 649, 1005, 1111, 1125, 1617, 2467, 2868, 3369
AjuI GAANNNNNNNTTGG 4 cut(s) 1838, 1870, 3281, 3313
AleI CACNNNNGTG 1 cut(s) 4343
Alw21I GWGCWC 5 cut(s) 2204, 2832, 3939, 3979, 4269
Alw26I GTCTC 7 cut(s) 122, 896, 1653, 3950, 4385, 4558, 4652
Alw44I GTGCAC 2 cut(s) 3975, 4265
AlwNI CAGNNNCTG 4 cut(s) 911, 2500, 3121, 4975
Ama87I CYCGRG 2 cut(s) 1485, 4790
ApaI GGGCCC 1 cut(s) 5090
ApaLI GTGCAC 2 cut(s) 3975, 4265
ApoI RAATTY 8 cut(s) 234, 1238, 1529, 2296, 2851, 3085, 3605, 3836
ArsI GACNNNNNNTTYG 4 cut(s) 442, 474, 1777, 1809
AseI ATTAAT 3 cut(s) 2367, 2835, 4299
AsiGI ACCGGT 1 cut(s) 2563
Asp700I GAANNNNTTC 1 cut(s) 3609
Asp718I GGTACC 1 cut(s) 3507
AspA2I CCTAGG 1 cut(s) 2185
AspLEI GCGC 1 cut(s) 2192
AsuC2I CCSGG 3 cut(s) 753, 3344, 4998
AsuHPI GGTGA 7 cut(s) 126, 431, 1681, 2395, 3605, 4688, 4924
AsuNHI GCTAGC 1 cut(s) 1393
AvaI CYCGRG 2 cut(s) 1485, 4790
AvaII GGWCC 6 cut(s) 274, 1123, 2917, 3037, 3197, 4201
AvrII CCTAGG 1 cut(s) 2185
AxyI CCTNAGG 1 cut(s) 1986
BaeGI GKGCMC 5 cut(s) 748, 1921, 3979, 4269, 5090
BaeI ACNNNNGTAYC 2 cut(s) 1026, 1059
BalI TGGCCA 2 cut(s) 1844, 3377
BamHI GGATCC 2 cut(s) 2212, 2779
BanI GGYRCC 3 cut(s) 743, 3507, 3758
BanII GRGCYC 6 cut(s) 2032, 2204, 2574, 2832, 3939, 5090
BarI GAAGNNNNNNTAC 2 cut(s) 3495, 3527
BauI CACGAG 1 cut(s) 3047
BbsI GAAGAC 4 cut(s) 1448, 2597, 3536, 4473
Bbv12I GWGCWC 5 cut(s) 2204, 2832, 3939, 3979, 4269
BbvCI CCTCAGC 2 cut(s) 1119, 3831
BccI CCATC 9 cut(s) 769, 1694, 3146, 3160, 3188, 3422, 3511, 4596, 5038
BceAI ACGGC 2 cut(s) 166, 3746
BcgI CGANNNNNNTGC 4 cut(s) 2410, 2444, 4832, 4866
BciT130I CCWGG 9 cut(s) 534, 651, 1007, 1113, 1127, 1619, 2469, 2870, 3371
BclI TGATCA 2 cut(s) 4513, 4678
BcnI CCSGG 3 cut(s) 753, 3344, 4998
BcoDI GTCTC 7 cut(s) 122, 896, 1653, 3950, 4385, 4558, 4652
BfaI CTAG 9 cut(s) 51, 374, 380, 899, 1394, 1409, 2186, 3722, 3962
BfmI CTRYAG 9 cut(s) 912, 1179, 1922, 2019, 3113, 3219, 3327, 4255, 4841
BfuAI ACCTGC 3 cut(s) 328, 1549, 4586
BglI GCCNNNNNGGC 1 cut(s) 209
BglII AGATCT 3 cut(s) 1432, 2047, 2398
BlnI CCTAGG 1 cut(s) 2185
BlpI GCTNAGC 1 cut(s) 3844
Bme18I GGWCC 6 cut(s) 274, 1123, 2917, 3037, 3197, 4201
BmeT110I CYCGRG 2 cut(s) 1485, 4790
BmgBI CACGTC 2 cut(s) 2357, 4568
BmrI ACTGGG 2 cut(s) 2129, 3721
BmtI GCTAGC 1 cut(s) 1397
BmuI ACTGGG 2 cut(s) 2129, 3721
BoxI GACNNNNGTC 1 cut(s) 2355
BpiI GAAGAC 4 cut(s) 1448, 2597, 3536, 4473
BplI GAGNNNNNCTC 2 cut(s) 4649, 4681
BpmI CTGGAG 2 cut(s) 2188, 2852
Bpu10I CCTNAGC 8 cut(s) 270, 333, 1119, 1767, 2712, 2913, 2997, 3831
Bpu1102I GCTNAGC 1 cut(s) 3844
BpuEI CTTGAG 3 cut(s) 237, 2054, 4559
BpuMI CCSGG 3 cut(s) 753, 3344, 4998
Bsa29I ATCGAT 2 cut(s) 159, 2955
BsaAI YACGTR 1 cut(s) 3467
BsaBI GATNNNNATC 1 cut(s) 3489
BsaJI CCNNGG 8 cut(s) 259, 650, 1191, 1617, 1618, 2185, 3378, 3534
BsaWI WCCGGW 1 cut(s) 2563
BsaXI ACNNNNNCTCC 2 cut(s) 1422, 1452
Bse118I RCCGGY 1 cut(s) 2563
Bse1I ACTGG 6 cut(s) 821, 2124, 2260, 2505, 3716, 4118
Bse21I CCTNAGG 1 cut(s) 1986
Bse3DI GCAATG 2 cut(s) 2822, 4614
Bse8I GATNNNNATC 1 cut(s) 3489
BseBI CCWGG 9 cut(s) 534, 651, 1007, 1113, 1127, 1619, 2469, 2870, 3371
BseCI ATCGAT 2 cut(s) 159, 2955
BseDI CCNNGG 8 cut(s) 259, 650, 1191, 1617, 1618, 2185, 3378, 3534
BseJI GATNNNNATC 1 cut(s) 3489
BseMI GCAATG 2 cut(s) 2822, 4614
BseNI ACTGG 6 cut(s) 821, 2124, 2260, 2505, 3716, 4118
BseRI GAGGAG 2 cut(s) 4475, 4946
BseSI GKGCMC 5 cut(s) 748, 1921, 3979, 4269, 5090
BseYI CCCAGC 3 cut(s) 747, 1262, 4950
BsgI GTGCAG 2 cut(s) 3453, 4974
Bsh1236I CGCG 2 cut(s) 1193, 2751
BshNI GGYRCC 3 cut(s) 743, 3507, 3758
BshTI ACCGGT 1 cut(s) 2563
BshVI ATCGAT 2 cut(s) 159, 2955
BsiHKAI GWGCWC 5 cut(s) 2204, 2832, 3939, 3979, 4269
BsiHKCI CYCGRG 2 cut(s) 1485, 4790
BsiSI CCGG 5 cut(s) 753, 2564, 3343, 4774, 4997
BslFI GGGAC 1 cut(s) 4214
BsmAI GTCTC 7 cut(s) 122, 896, 1653, 3950, 4385, 4558, 4652
BsmBI CGTCTC 1 cut(s) 4558
BsmFI GGGAC 1 cut(s) 4214
BsmI GAATGC 3 cut(s) 3338, 3698, 5079
BsoBI CYCGRG 2 cut(s) 1485, 4790
Bsp120I GGGCCC 1 cut(s) 5086
Bsp1407I TGTACA 1 cut(s) 2089
Bsp1720I GCTNAGC 1 cut(s) 3844
BspDI ATCGAT 2 cut(s) 159, 2955
BspFNI CGCG 2 cut(s) 1193, 2751
BspHI TCATGA 2 cut(s) 3493, 4681
BspMAI CTGCAG 4 cut(s) 916, 1183, 3117, 4845
BspMI ACCTGC 3 cut(s) 328, 1549, 4586
BspOI GCTAGC 1 cut(s) 1397
BspQI GCTCTTC 1 cut(s) 1416
BspT107I GGYRCC 3 cut(s) 743, 3507, 3758
BsrBI CCGCTC 1 cut(s) 448
BsrDI GCAATG 2 cut(s) 2822, 4614
BsrFI RCCGGY 1 cut(s) 2563
BsrGI TGTACA 1 cut(s) 2089
BsrI ACTGG 6 cut(s) 821, 2124, 2260, 2505, 3716, 4118
BssAI RCCGGY 1 cut(s) 2563
BssECI CCNNGG 8 cut(s) 259, 650, 1191, 1617, 1618, 2185, 3378, 3534
BssNAI GTATAC 1 cut(s) 2418
BssSI CACGAG 1 cut(s) 3047
BssT1I CCWWGG 4 cut(s) 259, 2185, 3378, 3534
Bst1107I GTATAC 1 cut(s) 2418
Bst2BI CACGAG 1 cut(s) 3047
Bst2UI CCWGG 9 cut(s) 534, 651, 1007, 1113, 1127, 1619, 2469, 2870, 3371
Bst6I CTCTTC 3 cut(s) 1416, 2562, 3888
BstAPI GCANNNNNTGC 6 cut(s) 184, 623, 632, 1187, 1514, 3440
BstAUI TGTACA 1 cut(s) 2089
BstBAI YACGTR 1 cut(s) 3467
BstDSI CCRYGG 1 cut(s) 1191
BstENI CCTNNNNNAGG 1 cut(s) 2579
BstFNI CGCG 2 cut(s) 1193, 2751
BstHHI GCGC 1 cut(s) 2192
BstMAI GTCTC 7 cut(s) 122, 896, 1653, 3950, 4385, 4558, 4652
BstNI CCWGG 9 cut(s) 534, 651, 1007, 1113, 1127, 1619, 2469, 2870, 3371
BstPAI GACNNNNGTC 1 cut(s) 2355
BstSFI CTRYAG 9 cut(s) 912, 1179, 1922, 2019, 3113, 3219, 3327, 4255, 4841
BstSLI GKGCMC 5 cut(s) 748, 1921, 3979, 4269, 5090
BstUI CGCG 2 cut(s) 1193, 2751
BstV2I GAAGAC 4 cut(s) 1448, 2597, 3536, 4473
BstX2I RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
BstXI CCANNNNNNTGG 4 cut(s) 3163, 3644, 4197, 4348
BstYI RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
BstZ17I GTATAC 1 cut(s) 2418
Bsu15I ATCGAT 2 cut(s) 159, 2955
Bsu36I CCTNAGG 1 cut(s) 1986
BsuTUI ATCGAT 2 cut(s) 159, 2955
BtgI CCRYGG 1 cut(s) 1191
BtrI CACGTC 2 cut(s) 2357, 4568
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 5 cut(s) 1089, 1452, 1932, 3559, 4111
BveI ACCTGC 3 cut(s) 328, 1549, 4586
CaiI CAGNNNCTG 4 cut(s) 911, 2500, 3121, 4975
CciI TCATGA 2 cut(s) 3493, 4681
CfoI GCGC 1 cut(s) 2192
Cfr10I RCCGGY 1 cut(s) 2563
Cfr42I CCGCGG 1 cut(s) 1194
ClaI ATCGAT 2 cut(s) 159, 2955
CseI GACGC 3 cut(s) 136, 176, 2360
CsiI ACCWGGT 1 cut(s) 1125
CspAI ACCGGT 1 cut(s) 2563
CspCI CAANNNNNGTGG 4 cut(s) 1263, 1298, 3033, 3068
DraI TTTAAA 1 cut(s) 643
DraIII CACNNNGTG 1 cut(s) 4328
DrdI GACNNNNNNGTC 2 cut(s) 1130, 2663
DseDI GACNNNNNNGTC 2 cut(s) 1130, 2663
EaeI YGGCCR 4 cut(s) 3, 1842, 3375, 4775
Eam1104I CTCTTC 3 cut(s) 1416, 2562, 3888
EarI CTCTTC 3 cut(s) 1416, 2562, 3888
Ecl136II GAGCTC 3 cut(s) 2202, 2830, 3937
Eco130I CCWWGG 4 cut(s) 259, 2185, 3378, 3534
Eco24I GRGCYC 6 cut(s) 2032, 2204, 2574, 2832, 3939, 5090
Eco32I GATATC 2 cut(s) 837, 1948
Eco47I GGWCC 6 cut(s) 274, 1123, 2917, 3037, 3197, 4201
Eco53kI GAGCTC 3 cut(s) 2202, 2830, 3937
Eco57I CTGAAG 6 cut(s) 981, 1688, 1766, 1772, 2063, 2538
Eco81I CCTNAGG 1 cut(s) 1986
Eco88I CYCGRG 2 cut(s) 1485, 4790
EcoICRI GAGCTC 3 cut(s) 2202, 2830, 3937
EcoNI CCTNNNNNAGG 1 cut(s) 2579
EcoO109I RGGNCCY 5 cut(s) 37, 1123, 2917, 3667, 5087
EcoRI GAATTC 3 cut(s) 234, 3605, 3836
EcoRII CCWGG 9 cut(s) 532, 649, 1005, 1111, 1125, 1617, 2467, 2868, 3369
EcoRV GATATC 2 cut(s) 837, 1948
EcoT14I CCWWGG 4 cut(s) 259, 2185, 3378, 3534
EcoT22I ATGCAT 1 cut(s) 729
EcoT38I GRGCYC 6 cut(s) 2032, 2204, 2574, 2832, 3939, 5090
ErhI CCWWGG 4 cut(s) 259, 2185, 3378, 3534
Esp3I CGTCTC 1 cut(s) 4558
FalI AAGNNNNNCTT 2 cut(s) 2328, 2360
FaqI GGGAC 1 cut(s) 4214
FauI CCCGC 2 cut(s) 1599, 3208
FauNDI CATATG 1 cut(s) 3981
FbaI TGATCA 2 cut(s) 4513, 4678
FblI GTMKAC 4 cut(s) 190, 2417, 3549, 4332
FriOI GRGCYC 6 cut(s) 2032, 2204, 2574, 2832, 3939, 5090
FspBI CTAG 9 cut(s) 51, 374, 380, 899, 1394, 1409, 2186, 3722, 3962
GlaI GCGC 1 cut(s) 2191
GsaI CCCAGC 3 cut(s) 751, 1266, 4954
GsuI CTGGAG 2 cut(s) 2188, 2852
HapII CCGG 5 cut(s) 753, 2564, 3343, 4774, 4997
HgaI GACGC 3 cut(s) 136, 176, 2360
HhaI GCGC 1 cut(s) 2192
Hin6I GCGC 1 cut(s) 2190
HinP1I GCGC 1 cut(s) 2190
HincII GTYRAC 4 cut(s) 191, 1886, 3550, 4333
HindII GTYRAC 4 cut(s) 191, 1886, 3550, 4333
HindIII AAGCTT 6 cut(s) 872, 963, 2342, 4034, 4082, 4535
HpaII CCGG 5 cut(s) 753, 2564, 3343, 4774, 4997
HphI GGTGA 7 cut(s) 126, 431, 1681, 2395, 3605, 4688, 4924
Hpy99I CGWCG 2 cut(s) 152, 192
HpyCH4IV ACGT 9 cut(s) 80, 598, 1348, 1954, 2356, 2658, 3466, 3797, 4567
HpySE526I ACGT 9 cut(s) 80, 598, 1348, 1954, 2356, 2658, 3466, 3797, 4567
HspAI GCGC 1 cut(s) 2190
KpnI GGTACC 1 cut(s) 3511
Ksp22I TGATCA 2 cut(s) 4513, 4678
KspI CCGCGG 1 cut(s) 1194
LguI GCTCTTC 1 cut(s) 1416
LmnI GCTCC 9 cut(s) 535, 2207, 2275, 2482, 2629, 3366, 3538, 3970, 5020
MabI ACCWGGT 1 cut(s) 1125
MaeI CTAG 9 cut(s) 51, 374, 380, 899, 1394, 1409, 2186, 3722, 3962
MaeII ACGT 9 cut(s) 80, 598, 1348, 1954, 2356, 2658, 3466, 3797, 4567
MbiI CCGCTC 1 cut(s) 448
MfeI CAATTG 4 cut(s) 1038, 1977, 3230, 3386
MflI RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
MlsI TGGCCA 2 cut(s) 1844, 3377
MluNI TGGCCA 2 cut(s) 1844, 3377
MlyI GAGTC 5 cut(s) 200, 1096, 3001, 3168, 3855
MmeI TCCRAC 5 cut(s) 1543, 1722, 3187, 3279, 3948
Mox20I TGGCCA 2 cut(s) 1844, 3377
Mph1103I ATGCAT 1 cut(s) 729
MroXI GAANNNNTTC 1 cut(s) 3609
MscI TGGCCA 2 cut(s) 1844, 3377
MslI CAYNNNNRTG 8 cut(s) 26, 161, 173, 299, 3614, 3878, 4290, 4343
Msp20I TGGCCA 2 cut(s) 1844, 3377
MspA1I CMGCKG 4 cut(s) 959, 1138, 1193, 2246
MspI CCGG 5 cut(s) 753, 2564, 3343, 4774, 4997
MunI CAATTG 4 cut(s) 1038, 1977, 3230, 3386
Mva1269I GAATGC 3 cut(s) 3338, 3698, 5079
MvaI CCWGG 9 cut(s) 534, 651, 1007, 1113, 1127, 1619, 2469, 2870, 3371
MvnI CGCG 2 cut(s) 1193, 2751
NciI CCSGG 3 cut(s) 753, 3344, 4998
NdeI CATATG 1 cut(s) 3981
NheI GCTAGC 1 cut(s) 1393
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 7 cut(s) 91, 382, 865, 1344, 1810, 2281, 4370
NsiI ATGCAT 1 cut(s) 729
OliI CACNNNNGTG 1 cut(s) 4343
PaeI GCATGC 2 cut(s) 3822, 4749
PaeR7I CTCGAG 2 cut(s) 1485, 4790
PagI TCATGA 2 cut(s) 3493, 4681
PasI CCCWGGG 1 cut(s) 1618
PciI ACATGT 1 cut(s) 2662
PciSI GCTCTTC 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 3 cut(s) 3338, 3698, 5079
PdmI GAANNNNTTC 1 cut(s) 3609
PflMI CCANNNNNTGG 3 cut(s) 1880, 3454, 5069
PfoI TCCNGGA 1 cut(s) 2467
PinAI ACCGGT 1 cut(s) 2563
PleI GAGTC 5 cut(s) 200, 1095, 3000, 3167, 3855
PpsI GAGTC 5 cut(s) 200, 1095, 3000, 3167, 3855
Ppu21I YACGTR 1 cut(s) 3467
PpuMI RGGWCCY 2 cut(s) 1123, 2917
PscI ACATGT 1 cut(s) 2662
PshAI GACNNNNGTC 1 cut(s) 2355
PshBI ATTAAT 3 cut(s) 2367, 2835, 4299
Psp124BI GAGCTC 3 cut(s) 2204, 2832, 3939
Psp5II RGGWCCY 2 cut(s) 1123, 2917
Psp6I CCWGG 9 cut(s) 532, 649, 1005, 1111, 1125, 1617, 2467, 2868, 3369
PspFI CCCAGC 3 cut(s) 747, 1262, 4950
PspGI CCWGG 9 cut(s) 532, 649, 1005, 1111, 1125, 1617, 2467, 2868, 3369
PspOMI GGGCCC 1 cut(s) 5086
PspPPI RGGWCCY 2 cut(s) 1123, 2917
PspXI VCTCGAGB 2 cut(s) 1485, 4790
PstI CTGCAG 4 cut(s) 916, 1183, 3117, 4845
PstNI CAGNNNCTG 4 cut(s) 911, 2500, 3121, 4975
PsuI RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
PvuII CAGCTG 2 cut(s) 959, 2246
RseI CAYNNNNRTG 8 cut(s) 26, 161, 173, 299, 3614, 3878, 4290, 4343
SacI GAGCTC 3 cut(s) 2204, 2832, 3939
SacII CCGCGG 1 cut(s) 1194
SalI GTCGAC 3 cut(s) 189, 3548, 4331
SapI GCTCTTC 1 cut(s) 1416
SchI GAGTC 5 cut(s) 200, 1096, 3001, 3168, 3855
SexAI ACCWGGT 1 cut(s) 1125
SfcI CTRYAG 9 cut(s) 912, 1179, 1922, 2019, 3113, 3219, 3327, 4255, 4841
Sfr274I CTCGAG 2 cut(s) 1485, 4790
Sfr303I CCGCGG 1 cut(s) 1194
SgrBI CCGCGG 1 cut(s) 1194
SinI GGWCC 6 cut(s) 274, 1123, 2917, 3037, 3197, 4201
SlaI CTCGAG 2 cut(s) 1485, 4790
SmiMI CAYNNNNRTG 8 cut(s) 26, 161, 173, 299, 3614, 3878, 4290, 4343
SmlI CTYRAG 5 cut(s) 252, 1485, 2033, 4538, 4790
SmoI CTYRAG 5 cut(s) 252, 1485, 2033, 4538, 4790
SphI GCATGC 2 cut(s) 3822, 4749
SspI AATATT 1 cut(s) 2765
SspMI CTAG 9 cut(s) 51, 374, 380, 899, 1394, 1409, 2186, 3722, 3962
SstI GAGCTC 3 cut(s) 2204, 2832, 3939
StyI CCWWGG 4 cut(s) 259, 2185, 3378, 3534
TaiI ACGT 9 cut(s) 83, 601, 1351, 1957, 2359, 2661, 3469, 3800, 4570
TaqII GACCGA 2 cut(s) 3336, 4511
TatI WGTACW 6 cut(s) 111, 2089, 2175, 2232, 3728, 4857
TauI GCSGC 7 cut(s) 8, 11, 1193, 2703, 5032, 5056, 5080
TscAI CASTG 5 cut(s) 1089, 1452, 1939, 3559, 4118
TseFI GTSAC 7 cut(s) 91, 382, 865, 1344, 1810, 2281, 4370
Tsp45I GTSAC 7 cut(s) 91, 382, 865, 1344, 1810, 2281, 4370
TspGWI ACGGA 4 cut(s) 1205, 1709, 3419, 4010
TspRI CASTG 5 cut(s) 1089, 1452, 1939, 3559, 4118
Van91I CCANNNNNTGG 3 cut(s) 1880, 3454, 5069
VneI GTGCAC 2 cut(s) 3975, 4265
VpaK11BI GGWCC 6 cut(s) 274, 1123, 2917, 3037, 3197, 4201
VspI ATTAAT 3 cut(s) 2367, 2835, 4299
XagI CCTNNNNNAGG 1 cut(s) 2579
XapI RAATTY 8 cut(s) 234, 1238, 1529, 2296, 2851, 3085, 3605, 3836
XcmI CCANNNNNNNNNTGG 2 cut(s) 2881, 3451
XhoI CTCGAG 2 cut(s) 1485, 4790
XmaJI CCTAGG 1 cut(s) 2185
XmiI GTMKAC 4 cut(s) 190, 2417, 3549, 4332
XmnI GAANNNNTTC 1 cut(s) 3609
XspI CTAG 9 cut(s) 51, 374, 380, 899, 1394, 1409, 2186, 3722, 3962
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.