Rroxscaffold_1G00010850

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002740
Physical Location & Seq
Reverse (-)
13806550 .. 13823002
16453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00010850.1

Sequence Viewer

Length: 5814 bp
ATGGAGAGAAGAGCGACGACACGTCGATTCTGTCCCTCTCTCTCTCACACTAAAAAGGCCACCCACAAGAATCGTGGGCACATCCAGACGTCCAAGGCATCTGCTTCTTCTGCTTCGCTTAATTTGGCGCCGTCCAGTTCTGTTGCGGCAGACGTGGACCAACCGTTTTTGCTCGGCACCATGCATGTGGGCCCCGCCAACATTCTACGTAAACACGAAGAAGCAAAGGGTTGCAGTGACGTCATCCGCGCCTTTTATCCACTTTGGAAAAATTCCAGTCAATCCCACACGGCTTCATGGATCAGAGGCAAGTGCGTAGGCAGAGGCGCGTTCGGCACGGTTAATATCGGAGTCGGCAAATCGGACGGTGAAGTGTTCGCCGTCAAGTCCGTGGATCTCGCCGCGTGTCTCCCCAGCCAGGCGGAGGCGCTCGAGAACGAAATCCGGATTCTCCGGTCGCTCTCCTCGCCGTACGTCGTGAAGTACCTGAACGACGATCACACGGCGTCGTATCGGAACTTGCATATGGAGTACTTGCCGGGCGGCACCGCCGGGGACTCTGCTGACGTGGACGAGAGTGTTGTACAGTCGCGGACGTGGTGCGTGGTGTCGGCGCTCAAGTATGTGCACTCCCGGGGGATTGTTCACTGCGACGTGAAAGGAAGAAATGTTCTGGTCGGGCGCTGTTCGGACCAGGCGAAGCTCGCGGATTTCGGTTCGGCCGTGGATTATATGTCGGGGGACGGGTGCAGGGGGAGGATCTCGCCACGTGGAAGCCCGCTTTGGATGGCGCCGGAGGTGATCAGCGGGGAGTATCAGGGGCCGGAGTCCGACGTGTGGTCGTTGGGGTGCACGGTGATCGAGATGGTCACCGGGAAGCCGGCGTGGGAGGACGAGGGAATGGAGACGCTTAGCCGGATCGGGTTGTCGGGCGGGTTGCCTGAGTTTCCGACCCGGTTGTCCGAAACGGGTCGGGATTTTCTGGACAAGTGTCTGAGGAGAGACCCGAAGGAGAGGTGGAGCTGCGATCAGCTACTGCAGCACCCATTTCTGGCGTCAGTTTCGCCCAATGCGGCGGCGGATTCTTCGCCGCGGTGCGTGCTCGACTGGGTCAACTCGGAGTTCGAAGACGACGAGTACGAAAACGACGAAGAGAGTTGTTCTATTCACGAGGTTTCCGCTAAGGAGCGGATTGGTAAATTGGCCTCGAACTCAGGGGTAGATTGGGAATTTTCAGATGGGTGGACGGTGGTGCGGGGCGCAGTAGAAGTAGTGGAATCCGGCGCTTTTTCGCGGGAAGAGACGAGTTCGGGATATTCAGATTGTGGAGGGGCATTTTCGGAAGGTGAAAGTTGCGGTGGCGGTGATTTAGTGGAGTGGGCACGTAATGAGTGTGAAACGGTTGGCTCGAGTGGTTGTTGGTATGGGCCGTCGGTGATGGAATATTCAGCGATCTGCACTCTCTCTCAGAGCTTCGCCAATCGGGTCGGTCCATCCCTTCGAAGCCCCGATTCAGGCGCCATGGCCGCCGCTGCCAATGCCCCCATGACCATGAAGGAGCAGCTCACGCTGCCTAGTATTGGGATTAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGCGTTAGGGAGACCTCGCCGCAGAATAGCATCGTGATCGTTGACATGAACATGCCAATGCAGCCGTTACGGCGGCCGATTACTGCTGATTCGGCTTTGATGAATCCTAATTCGAGGATTCTGGCTCTTAAAGCTCAAGTGCAAGGAACTACTCAGGATCATTTACAGATATTTAACATTGAACTGAAAACAAAGATGAAATCACATCAGATGCCTGAGCAGATCGTGTTTTGGAAGTGGGTCACTCCTAAGATGCTGGGGATGGTCACACAGACCTCAGTATACCACTGGTTAATTGACGGGGAGTCTGAGCCAACAAAGGTGTTTGAGCGTACAGCTAATTTAGCAAATAATCAAATTATCAACTACCGTTGTGATCCCTCTGAAAAGTGGTTGGTCTTAATTGGAATTGCTCCTGGTTCTCCTGAGAGACCACAACTGGTTAAAGGAAACATGCAGCTTTTCTCTGTGGAGCAGCAACGTAGTCAGGCTCTTGAAGCACATGCTGCCTCATTTGCCCCATTTAAAGTTGCAGGGAACGAGAACCCTTCAATTCTTATATGCTTTGCTGCGAAGTCCTCCAACGCTGGACAAGTTACATCAAAGTTGCATGTTATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATATACTAAGAAGCAAGCAGATCTATTCTTTCCTCCGGATTTTGCTGATGACTTTCCTGTGTCAATGCAGATATCAGAAAAGTATGGGTTGATATATGTTATCACAAAGCTTGGACTACTGTTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCGGATCCTATATTTTTGACAGCTGAAGCTTCATCTGTTGGAGGTTTTTATGCAGTCAACAGGCGTGGTCAGGTGTTACTTGCCACTGTAAATGAACAAACAATTGTACCTTTTGTTAGTGGTCAGTTAAACAATTTGGGACTCGCAGTTAGTCTTGCCAGAAGAGGAAATCTTCCTGGTGCTGAAGACTTGGTTGTCCAGCGGTTCCAAGAATTGTTTTCTCAGACAAAATATAAGGAGGCTGCAGAGCTTGCTGCAGATTCTCCAATGGGAGTACTCCGTACACCTGAAACTGTTGCTAAATTTCAGGGTGTTCCTGTGCAAGCTGGGCAAACACCACCATTGTTGCAGTACTTTGGAACTTTGTTAACTAAAGGGAAACTCAACACTTTTGAATCATTGGAACTGTCACGTTTGGTTGTAAATCAAAACAAGAAGAATCTTTTGGAGAATTGGCTAGCCGAGGACAAGCTTGAATGTAGCGAAGAGCTAGGAGATCTTGTGAAGTACATAATTTTTCATTGCAAAGAAAGAAACACTTTGAGAATTGCGTTTGAGCTTGGAGCTTGGAGATCTACGAAGGTTGGATATACACCTGATTATATGTTCCTTCTGCAAACAATTTTGCGGACAGATGCCCAGGGGGCAGTCAATTTTGCACTAATGATGTCTCAAATGGAAGGAGGTTGCCCTATTGACTACAATACCATTACTGATCTGTTCCTTCAGAGGAATTTGATTCGTGAGGCAACAGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCTGAGCATGCCCACCTTCAGACAAAGGTTTTGGAAATCAATCTAGTGACTTTTCCCAATGTTGCGGATGCTATATTGGCCAATGGTATTGAGGATCCCGATATCCACTTTAAGTATATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGAGTGACTCGTGAATCTAATTTTTATGATGCTGAGAAGGCTAAGAACTTTCTAATGGAAGCAAAGCTTCCAGATGCGCGTCCCCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCTGATCTCACTCATTACCTATATACGAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTTGGGCAGCTTTTGGATGATGAATGTCCTGAAGATTTCATCAAAGGACTTATTCTATCAGTTCGTTCTCTTCTACCAGTCGAGCCCCTTGTTGATGAATGTGAAAAGAGGAATCGTCTTCGCCTGCTCACTCAGTTCTTGGAGCATCTTGTGAGCGAGGGAAGCCAAGATGTACATGTTCATAATGCTCTTGTTTCTGCAACTGTTAAAGCGTTTATGACCGCGGACCTTCCACATGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTCCAAAATTCAGCTTTTAGTGGGAACTTCAATCTACAGAATCTGTTGATCTTGACTGCAATTAAGGCTGATCCATCCAGAGTCATGGATTATATCAATAGATTAGACAACTTTGATGGGCCTGCAGTTGGAGTAATGGCTGTAGAAGCCCAACTCTATGAGGAAGCATTTGCAATCTTTAAGAAGTTCAATTTGAACGTTGATGCTGTCAATGTTCTGTTGGATAACATTCAGAGCATTGACCGTGCCGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTTGCCAAGGCCCAACTACGAGAGGGGCTTGTGAGTGATGCTATTGAGTCATTCATTCGTGCTGATGATGCCACCCAGTTTTTGGATGTCATACGTGCTGCTCAGGATGCAGATGTGTATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGGCGAAAGAGCCCAAGGTAGACAGTGAACTCATTTATGCATATGCTAAGATTGATCGACTTGGTGACATTGAAGAGTTCATTCTTATGCCAAATGTTGCTAATCTCCCAAATGTTGGGGATCGCCTGTATGATGAAGAACTATATGAAGCAGCCAAGATCATATTTGCATTTATTTCTAATTGGGCCAAGCTGGCGTGTACTCTAGTGAAGCTCAAGCAGTTTCAAGGTGCTGTTGATGCTGCCCGGAAAGCTAACAGCTCAAAAACTTGGAAGGAAGTTTGCTTTGCATGTGTTGATGCTGAGGAATTCCGGTTGGCTCAAATTTGTGGACTCAACATCATCATCCAGGTGGATGATTTGGAAGAGGTCAGATTGGAACGTGCACATATGGGCATCTTTACTGAGTTAGGAGTTCTATATGCAAGATACCGTCCTGAGAAGCTTATGGAGCACATTAAACTGTTTTCTACTCGTCTCAACATTCCCAAACTTATACGAGCTTGTGATGAACAGCAGCACTGGAAGGAATTAACATACTTATATATACAATACGATGAATTTGATAATGCTGCAACCACCATAATGAACCACTCCCCAGAAGCATGGGAACACATGCAATTCAAGGATGTGATAGTCAAAGTTGCAAGTGTTGAGCTTTACTATAAGGCTGTGCACTTTTACTTAGAAGAACATCCTGATATTATCAATGATGTTCTAAATGTGCTTGCACTTCGTGTGGATCATACCCCGAGTGGTGGATATAATGCGAAAGTCCAGAGCAACAATGTAACAGCTGTAAATGAAGCTCTAAATGAAATATATATTGAGGAGGAAGACTATGACAGACTTCGTGAATCTATAGATTTGCATGATAGCTTTGACCAGATAGGTCTTGCACAAAAGCTTGAGAAACATGAGCTTCTTGAGATGAGACGTGTTGCTGCATACATTTATAAGAAAGCTGGAAGATGGAAGCAATCCATTGCATTGTCAAAGAAAGACAAACATTACAAAGACGCCATGGAGACATGCTCACAGTCTGGTGATCATGAACTTTCAGAGGAATTGCTTGTCTATTTCATCGAGCAGGGAAAGAAAGAATGTTTCGCATCGTGTCTCTTTGTTTGTTATGATTTAATCCGGCCAGACGTTGCTCTTGAGCTGGCATGGATGAACAATATAATTGATTTTGCATTCCCATATATCCTGCAGTTCATTCGTGAGTACACAGGCAAGGTTGATGAACTTGTCAAAGACAAAATTGAGACTCAGAAAGAGGTGAAAGCCAAAGAGCAAGAGGAGAAGGAATTGGTTGCTAAGCAGAACATGTATGCTCAGTTGCTGCCTGCTTTGCCTGCACCTCCGGGAATGGGTGGAGGTTACGCTCCACCTCCAATGCCACCAATGCCACAGATGGGTGGGATGGGGATGCCTCCAATGCCACCTTTCGGAATGCCACAAATGGGCCCTTCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1937

Amino Acids

216.42

Weight (kDa)

5.23

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 103 - 351 1.8e-54 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 104 - 347 1.6e-26 Protein tyrosine and serine/threonine kinase
Clathrin_propel PF01394 528 - 564 2.9e-06 Clathrin propeller repeat
Clathrin-link PF09268 852 - 874 7.2e-08 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 877 - 942 1.4e-26 Clathrin-H-link
Clathrin PF00637 1051 - 1109 2.3e-07 Region in Clathrin and VPS
Clathrin PF00637 1108 - 1217 1.5e-17 Region in Clathrin and VPS
Clathrin PF00637 1226 - 1274 2.2e-06 Region in Clathrin and VPS
Clathrin PF00637 1270 - 1399 1.9e-29 Region in Clathrin and VPS
Clathrin PF00637 1412 - 1549 8e-30 Region in Clathrin and VPS
Clathrin PF00637 1565 - 1678 4.7e-20 Region in Clathrin and VPS
Clathrin PF00637 1704 - 1813 1.7e-23 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5289
AasI GACNNNNNNGTC 2 cut(s) 958, 2654
AatII GACGTC 2 cut(s) 92, 243
Acc65I GGTACC 1 cut(s) 4311
AccB1I GGYRCC 7 cut(s) 127, 176, 545, 790, 1517, 2267, 4311
AccB7I CCANNNNNTGG 2 cut(s) 4258, 4448
AccBSI CCGCTC 1 cut(s) 1189
AccI GTMKAC 2 cut(s) 1923, 4353
AccII CGCG 9 cut(s) 249, 329, 404, 592, 707, 1093, 1294, 3484, 3839
AccIII TCCGGA 3 cut(s) 444, 2323, 2461
AclI AACGTT 1 cut(s) 4080
AcoI YGGCCR 5 cut(s) 720, 1524, 1715, 3324, 5477
AcsI RAATTY 8 cut(s) 271, 1229, 2762, 3193, 3889, 4640, 4656, 4892
AcuI CTGAAG 6 cut(s) 2505, 2664, 3170, 3248, 3254, 3666
AcvI CACGTG 1 cut(s) 770
AcyI GRCGYC 8 cut(s) 89, 128, 240, 506, 791, 1055, 1518, 5352
AdeI CACNNNGTG 1 cut(s) 5069
AflIII ACRYGT 5 cut(s) 20, 834, 3790, 5269, 5661
AhdI GACNNNNNGTC 3 cut(s) 21, 838, 1945
AjiI CACGTC 7 cut(s) 23, 154, 568, 597, 655, 835, 5270
AjnI CCWGG 9 cut(s) 417, 693, 2056, 2275, 2635, 3099, 3595, 4173, 4680
AjuI GAANNNNNNNTTGG 4 cut(s) 766, 798, 2888, 2920
AloI GAACNNNNNNTCC 2 cut(s) 654, 686
Alw21I GWGCWC 7 cut(s) 630, 854, 1104, 3885, 4720, 4788, 5010
Alw44I GTGCAC 4 cut(s) 626, 850, 4716, 5006
AlwNI CAGNNNCTG 4 cut(s) 1036, 2435, 3925, 5677
Ama87I CYCGRG 4 cut(s) 431, 633, 1408, 5083
Aor13HI TCCGGA 3 cut(s) 444, 2323, 2461
ApaI GGGCCC 2 cut(s) 194, 5804
ApaLI GTGCAC 4 cut(s) 626, 850, 4716, 5006
ApoI RAATTY 8 cut(s) 271, 1229, 2762, 3193, 3889, 4640, 4656, 4892
ArsI GACNNNNNNTTYG 2 cut(s) 3259, 3291
AseI ATTAAT 2 cut(s) 1587, 3495
Asp718I GGTACC 1 cut(s) 4311
AsuC2I CCSGG 9 cut(s) 540, 553, 634, 635, 874, 955, 4148, 4579, 5700
AsuII TTCGAA 2 cut(s) 1125, 1501
AsuNHI GCTAGC 1 cut(s) 2917
AvaI CYCGRG 4 cut(s) 431, 633, 1408, 5083
AvaII GGWCC 4 cut(s) 157, 691, 1490, 3841
BaeGI GKGCMC 9 cut(s) 81, 194, 630, 854, 1384, 2272, 4720, 5010, 5804
BaeI ACNNNNGTAYC 2 cut(s) 2550, 2583
BalI TGGCCA 1 cut(s) 3326
BamHI GGATCC 2 cut(s) 2464, 3340
BanI GGYRCC 7 cut(s) 127, 176, 545, 790, 1517, 2267, 4311
BanII GRGCYC 4 cut(s) 194, 3702, 4347, 5804
BarI GAAGNNNNNNTAC 2 cut(s) 4299, 4331
BauI CACGAG 2 cut(s) 1169, 3414
BbrPI CACGTG 1 cut(s) 770
BbsI GAAGAC 4 cut(s) 1134, 2652, 3725, 5175
Bbv12I GWGCWC 7 cut(s) 630, 854, 1104, 3885, 4720, 4788, 5010
BbvCI CCTCAGC 1 cut(s) 4635
BcgI CGANNNNNNTGC 8 cut(s) 841, 875, 1660, 1694, 3538, 3572, 5534, 5568
BciT130I CCWGG 9 cut(s) 419, 695, 2058, 2277, 2637, 3101, 3597, 4175, 4682
BclI TGATCA 2 cut(s) 801, 5380
BcnI CCSGG 9 cut(s) 540, 553, 634, 635, 874, 955, 4148, 4579, 5700
BfaI CTAG 6 cut(s) 1575, 2423, 2918, 2951, 3290, 4538
BfmI CTRYAG 9 cut(s) 1037, 2436, 2703, 2715, 3917, 4005, 4023, 5193, 5543
BfoI RGCGCY 7 cut(s) 131, 431, 617, 685, 794, 1287, 1521
BglI GCCNNNNNGGC 3 cut(s) 1711, 3104, 4526
BglII AGATCT 3 cut(s) 2308, 2956, 3032
BlpI GCTNAGC 2 cut(s) 911, 5652
BmcAI AGTACT 3 cut(s) 533, 2736, 2813
Bme18I GGWCC 4 cut(s) 157, 691, 1490, 3841
BmeRI GACNNNNNGTC 3 cut(s) 21, 838, 1945
BmeT110I CYCGRG 4 cut(s) 431, 633, 1408, 5083
BmgBI CACGTC 7 cut(s) 23, 154, 568, 597, 655, 835, 5270
BmrI ACTGGG 2 cut(s) 1117, 4246
BmtI GCTAGC 1 cut(s) 2921
BmuI ACTGGG 2 cut(s) 1117, 4246
BoxI GACNNNNGTC 1 cut(s) 990
BpiI GAAGAC 4 cut(s) 1134, 2652, 3725, 5175
BplI GAGNNNNNCTC 2 cut(s) 5351, 5383
Bpu10I CCTNAGC 5 cut(s) 1182, 1857, 3249, 4278, 4635
Bpu1102I GCTNAGC 2 cut(s) 911, 5652
Bpu14I TTCGAA 2 cut(s) 1125, 1501
BpuEI CTTGAG 6 cut(s) 602, 1761, 4532, 5261, 5279, 5513
BpuMI CCSGG 9 cut(s) 540, 553, 634, 635, 874, 955, 4148, 4579, 5700
BsaAI YACGTR 4 cut(s) 209, 770, 1385, 4271
BsaBI GATNNNNATC 1 cut(s) 4293
BsaHI GRCGYC 8 cut(s) 89, 128, 240, 506, 791, 1055, 1518, 5352
BsaI GGTCTC 3 cut(s) 996, 1646, 2065
BsaWI WCCGGW 5 cut(s) 444, 453, 2323, 2461, 4644
BsaXI ACNNNNNCTCC 2 cut(s) 2946, 2976
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 9 cut(s) 135, 276, 1112, 1934, 2085, 3388, 3692, 4252, 4859
Bse3DI GCAATG 2 cut(s) 2980, 5316
Bse8I GATNNNNATC 1 cut(s) 4293
BseAI TCCGGA 3 cut(s) 444, 2323, 2461
BseBI CCWGG 9 cut(s) 419, 695, 2058, 2277, 2637, 3101, 3597, 4175, 4682
BseJI GATNNNNATC 1 cut(s) 4293
BseMI GCAATG 2 cut(s) 2980, 5316
BseNI ACTGG 9 cut(s) 135, 276, 1112, 1934, 2085, 3388, 3692, 4252, 4859
BseRI GAGGAG 4 cut(s) 454, 1012, 5177, 5648
BseSI GKGCMC 9 cut(s) 81, 194, 630, 854, 1384, 2272, 4720, 5010, 5804
BseX3I CGGCCG 2 cut(s) 720, 1715
BseYI CCCAGC 4 cut(s) 413, 1897, 2271, 2786
BsgI GTGCAG 3 cut(s) 769, 1441, 5676
Bsh1236I CGCG 9 cut(s) 249, 329, 404, 592, 707, 1093, 1294, 3484, 3839
Bsh1285I CGRYCG 3 cut(s) 458, 723, 1718
BshNI GGYRCC 7 cut(s) 127, 176, 545, 790, 1517, 2267, 4311
BsiEI CGRYCG 3 cut(s) 458, 723, 1718
BsiHKAI GWGCWC 7 cut(s) 630, 854, 1104, 3885, 4720, 4788, 5010
BsiHKCI CYCGRG 4 cut(s) 431, 633, 1408, 5083
BsiWI CGTACG 1 cut(s) 471
BslFI GGGAC 5 cut(s) 18, 569, 755, 2613, 3471
BsmBI CGTCTC 4 cut(s) 899, 1295, 4814, 5260
BsmFI GGGAC 5 cut(s) 18, 569, 755, 2613, 3471
BsmI GAATGC 3 cut(s) 4142, 5528, 5793
Bso31I GGTCTC 3 cut(s) 996, 1646, 2065
BsoBI CYCGRG 4 cut(s) 431, 633, 1408, 5083
Bsp119I TTCGAA 2 cut(s) 1125, 1501
Bsp120I GGGCCC 2 cut(s) 190, 5800
Bsp13I TCCGGA 3 cut(s) 444, 2323, 2461
Bsp1407I TGTACA 2 cut(s) 583, 3787
Bsp1720I GCTNAGC 2 cut(s) 911, 5652
Bsp19I CCATGG 2 cut(s) 1521, 5355
BspEI TCCGGA 3 cut(s) 444, 2323, 2461
BspFNI CGCG 9 cut(s) 249, 329, 404, 592, 707, 1093, 1294, 3484, 3839
BspHI TCATGA 2 cut(s) 4297, 5383
BspMAI CTGCAG 6 cut(s) 1041, 2440, 2707, 2719, 4009, 5547
BspOI GCTAGC 1 cut(s) 2921
BspQI GCTCTTC 2 cut(s) 4, 2940
BspT104I TTCGAA 2 cut(s) 1125, 1501
BspT107I GGYRCC 7 cut(s) 127, 176, 545, 790, 1517, 2267, 4311
BspTNI GGTCTC 3 cut(s) 996, 1646, 2065
BsrBI CCGCTC 1 cut(s) 1189
BsrDI GCAATG 2 cut(s) 2980, 5316
BsrFI RCCGGY 1 cut(s) 880
BsrGI TGTACA 2 cut(s) 583, 3787
BsrI ACTGG 9 cut(s) 135, 276, 1112, 1934, 2085, 3388, 3692, 4252, 4859
BssAI RCCGGY 1 cut(s) 880
BssNAI GTATAC 1 cut(s) 1924
BssNI GRCGYC 8 cut(s) 89, 128, 240, 506, 791, 1055, 1518, 5352
BssSI CACGAG 2 cut(s) 1169, 3414
BssT1I CCWWGG 5 cut(s) 93, 1521, 4182, 4347, 5355
Bst1107I GTATAC 1 cut(s) 1924
Bst2BI CACGAG 2 cut(s) 1169, 3414
Bst2UI CCWGG 9 cut(s) 419, 695, 2058, 2277, 2637, 3101, 3597, 4175, 4682
Bst6I CTCTTC 8 cut(s) 4, 1146, 1293, 2617, 2940, 3690, 4401, 4692
BstACI GRCGYC 8 cut(s) 89, 128, 240, 506, 791, 1055, 1518, 5352
BstAPI GCANNNNNTGC 2 cut(s) 2147, 2711
BstAUI TGTACA 2 cut(s) 583, 3787
BstBAI YACGTR 4 cut(s) 209, 770, 1385, 4271
BstBI TTCGAA 2 cut(s) 1125, 1501
BstDSI CCRYGG 6 cut(s) 390, 723, 1091, 1521, 3837, 5355
BstEII GGTNACC 1 cut(s) 868
BstFNI CGCG 9 cut(s) 249, 329, 404, 592, 707, 1093, 1294, 3484, 3839
BstH2I RGCGCY 7 cut(s) 131, 431, 617, 685, 794, 1287, 1521
BstMCI CGRYCG 3 cut(s) 458, 723, 1718
BstNI CCWGG 9 cut(s) 419, 695, 2058, 2277, 2637, 3101, 3597, 4175, 4682
BstPAI GACNNNNGTC 1 cut(s) 990
BstPI GGTNACC 1 cut(s) 868
BstSFI CTRYAG 9 cut(s) 1037, 2436, 2703, 2715, 3917, 4005, 4023, 5193, 5543
BstSLI GKGCMC 9 cut(s) 81, 194, 630, 854, 1384, 2272, 4720, 5010, 5804
BstSNI TACGTA 1 cut(s) 209
BstUI CGCG 9 cut(s) 249, 329, 404, 592, 707, 1093, 1294, 3484, 3839
BstV2I GAAGAC 4 cut(s) 1134, 2652, 3725, 5175
BstX2I RGATCY 7 cut(s) 394, 759, 2308, 2464, 2956, 3032, 3340
BstXI CCANNNNNNTGG 3 cut(s) 187, 3967, 4938
BstYI RGATCY 7 cut(s) 394, 759, 2308, 2464, 2956, 3032, 3340
BstZ17I GTATAC 1 cut(s) 1924
BstZI CGGCCG 2 cut(s) 720, 1715
BtgI CCRYGG 6 cut(s) 390, 723, 1091, 1521, 3837, 5355
BtrI CACGTC 7 cut(s) 23, 154, 568, 597, 655, 835, 5270
BtsI GCAGTG 2 cut(s) 241, 646
BtsIMutI CAGTG 6 cut(s) 241, 646, 1927, 2544, 4363, 4852
CaiI CAGNNNCTG 4 cut(s) 1036, 2435, 3925, 5677
CciI TCATGA 2 cut(s) 4297, 5383
Cfr10I RCCGGY 1 cut(s) 880
Cfr42I CCGCGG 2 cut(s) 1094, 3840
Cfr9I CCCGGG 1 cut(s) 633
CseI GACGC 5 cut(s) 495, 916, 1044, 3473, 5360
CspCI CAANNNNNGTGG 8 cut(s) 55, 90, 2506, 2541, 2787, 2822, 3837, 3872
DinI GGCGCC 3 cut(s) 129, 792, 1519
DraI TTTAAA 1 cut(s) 2167
DraIII CACNNNGTG 1 cut(s) 5069
DrdI GACNNNNNNGTC 2 cut(s) 958, 2654
DriI GACNNNNNGTC 3 cut(s) 21, 838, 1945
DseDI GACNNNNNNGTC 2 cut(s) 958, 2654
EaeI YGGCCR 5 cut(s) 720, 1524, 1715, 3324, 5477
EagI CGGCCG 2 cut(s) 720, 1715
Eam1104I CTCTTC 8 cut(s) 4, 1146, 1293, 2617, 2940, 3690, 4401, 4692
Eam1105I GACNNNNNGTC 3 cut(s) 21, 838, 1945
EarI CTCTTC 8 cut(s) 4, 1146, 1293, 2617, 2940, 3690, 4401, 4692
EciI GGCGGA 2 cut(s) 437, 1094
EclXI CGGCCG 2 cut(s) 720, 1715
Eco105I TACGTA 1 cut(s) 209
Eco130I CCWWGG 5 cut(s) 93, 1521, 4182, 4347, 5355
Eco24I GRGCYC 4 cut(s) 194, 3702, 4347, 5804
Eco31I GGTCTC 3 cut(s) 996, 1646, 2065
Eco32I GATATC 2 cut(s) 2361, 3349
Eco47I GGWCC 4 cut(s) 157, 691, 1490, 3841
Eco52I CGGCCG 2 cut(s) 720, 1715
Eco57I CTGAAG 6 cut(s) 2505, 2664, 3170, 3248, 3254, 3666
Eco72I CACGTG 1 cut(s) 770
Eco88I CYCGRG 4 cut(s) 431, 633, 1408, 5083
Eco91I GGTNACC 1 cut(s) 868
EcoO109I RGGNCCY 2 cut(s) 191, 5801
EcoO65I GGTNACC 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 4640
EcoRII CCWGG 9 cut(s) 417, 693, 2056, 2275, 2635, 3099, 3595, 4173, 4680
EcoRV GATATC 2 cut(s) 2361, 3349
EcoT14I CCWWGG 5 cut(s) 93, 1521, 4182, 4347, 5355
EcoT22I ATGCAT 2 cut(s) 186, 4375
EcoT38I GRGCYC 4 cut(s) 194, 3702, 4347, 5804
EgeI GGCGCC 3 cut(s) 129, 792, 1519
EheI GGCGCC 3 cut(s) 129, 792, 1519
ErhI CCWWGG 5 cut(s) 93, 1521, 4182, 4347, 5355
Esp3I CGTCTC 4 cut(s) 899, 1295, 4814, 5260
FalI AAGNNNNNCTT 4 cut(s) 2983, 3015, 3456, 3488
FaqI GGGAC 5 cut(s) 18, 569, 755, 2613, 3471
FauI CCCGC 6 cut(s) 202, 786, 800, 926, 1248, 1287
FauNDI CATATG 3 cut(s) 525, 4375, 4722
FbaI TGATCA 2 cut(s) 801, 5380
FblI GTMKAC 2 cut(s) 1923, 4353
FriOI GRGCYC 4 cut(s) 194, 3702, 4347, 5804
FspBI CTAG 6 cut(s) 1575, 2423, 2918, 2951, 3290, 4538
GsaI CCCAGC 4 cut(s) 417, 1901, 2275, 2790
HaeII RGCGCY 7 cut(s) 131, 431, 617, 685, 794, 1287, 1521
HgaI GACGC 5 cut(s) 495, 916, 1044, 3473, 5360
Hin1I GRCGYC 8 cut(s) 89, 128, 240, 506, 791, 1055, 1518, 5352
HincII GTYRAC 4 cut(s) 1114, 1684, 2518, 2829
HindII GTYRAC 4 cut(s) 1114, 1684, 2518, 2829
HindIII AAGCTT 6 cut(s) 2396, 2487, 2930, 3470, 4775, 5237
HpaI GTTAAC 1 cut(s) 2829
Hsp92I GRCGYC 8 cut(s) 89, 128, 240, 506, 791, 1055, 1518, 5352
KasI GGCGCC 3 cut(s) 127, 790, 1517
Kpn2I TCCGGA 3 cut(s) 444, 2323, 2461
KpnI GGTACC 1 cut(s) 4315
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
Ksp22I TGATCA 2 cut(s) 801, 5380
KspAI GTTAAC 1 cut(s) 2829
KspI CCGCGG 2 cut(s) 1094, 3840
LguI GCTCTTC 2 cut(s) 4, 2940
MaeI CTAG 6 cut(s) 1575, 2423, 2918, 2951, 3290, 4538
MbiI CCGCTC 1 cut(s) 1189
MfeI CAATTG 1 cut(s) 2562
MflI RGATCY 7 cut(s) 394, 759, 2308, 2464, 2956, 3032, 3340
MlsI TGGCCA 1 cut(s) 3326
MluNI TGGCCA 1 cut(s) 3326
Mly113I GGCGCC 3 cut(s) 128, 791, 1518
MmeI TCCRAC 8 cut(s) 855, 974, 2247, 2479, 3025, 3204, 3991, 4083
Mox20I TGGCCA 1 cut(s) 3326
Mph1103I ATGCAT 2 cut(s) 186, 4375
MroI TCCGGA 3 cut(s) 444, 2323, 2461
MroNI GCCGGC 1 cut(s) 880
MscI TGGCCA 1 cut(s) 3326
MslI CAYNNNNRTG 7 cut(s) 185, 1550, 1691, 1697, 4418, 4682, 4916
Msp20I TGGCCA 1 cut(s) 3326
MspA1I CMGCKG 8 cut(s) 807, 1093, 1532, 2483, 2662, 3374, 3839, 5129
MunI CAATTG 1 cut(s) 2562
Mva1269I GAATGC 3 cut(s) 4142, 5528, 5793
MvaI CCWGG 9 cut(s) 419, 695, 2058, 2277, 2637, 3101, 3597, 4175, 4682
MvnI CGCG 9 cut(s) 249, 329, 404, 592, 707, 1093, 1294, 3484, 3839
NaeI GCCGGC 1 cut(s) 882
NarI GGCGCC 3 cut(s) 128, 791, 1518
NciI CCSGG 9 cut(s) 540, 553, 634, 635, 874, 955, 4148, 4579, 5700
NcoI CCATGG 2 cut(s) 1521, 5355
NdeI CATATG 3 cut(s) 525, 4375, 4722
NgoMIV GCCGGC 1 cut(s) 880
NheI GCTAGC 1 cut(s) 2917
NmeAIII GCCGAG 2 cut(s) 153, 2947
NmuCI GTSAC 9 cut(s) 236, 868, 1615, 1882, 1906, 2868, 3292, 3409, 4397
NsiI ATGCAT 2 cut(s) 186, 4375
NspV TTCGAA 2 cut(s) 1125, 1501
PaeI GCATGC 1 cut(s) 3257
PaeR7I CTCGAG 2 cut(s) 431, 1408
PagI TCATGA 2 cut(s) 4297, 5383
PasI CCCWGGG 1 cut(s) 3100
PciI ACATGT 2 cut(s) 3790, 5661
PciSI GCTCTTC 2 cut(s) 4, 2940
PcsI WCGNNNNNNNCGW 6 cut(s) 695, 720, 1131, 1137, 1146, 1406
PctI GAATGC 3 cut(s) 4142, 5528, 5793
PdiI GCCGGC 1 cut(s) 882
Pfl23II CGTACG 1 cut(s) 471
PflFI GACNNNGTC 1 cut(s) 1109
PflMI CCANNNNNTGG 2 cut(s) 4258, 4448
PfoI TCCNGGA 2 cut(s) 3595, 5698
PluTI GGCGCC 3 cut(s) 131, 794, 1521
PmaCI CACGTG 1 cut(s) 770
PmlI CACGTG 1 cut(s) 770
Ppu21I YACGTR 4 cut(s) 209, 770, 1385, 4271
PscI ACATGT 2 cut(s) 3790, 5661
PshAI GACNNNNGTC 1 cut(s) 990
PshBI ATTAAT 2 cut(s) 1587, 3495
PsiI TTATAA 1 cut(s) 5289
Psp1406I AACGTT 1 cut(s) 4080
Psp6I CCWGG 9 cut(s) 417, 693, 2056, 2275, 2635, 3099, 3595, 4173, 4680
PspCI CACGTG 1 cut(s) 770
PspEI GGTNACC 1 cut(s) 868
PspFI CCCAGC 4 cut(s) 413, 1897, 2271, 2786
PspGI CCWGG 9 cut(s) 417, 693, 2056, 2275, 2635, 3099, 3595, 4173, 4680
PspLI CGTACG 1 cut(s) 471
PspOMI GGGCCC 2 cut(s) 190, 5800
PspXI VCTCGAGB 1 cut(s) 1408
PstI CTGCAG 6 cut(s) 1041, 2440, 2707, 2719, 4009, 5547
PstNI CAGNNNCTG 4 cut(s) 1036, 2435, 3925, 5677
PsuI RGATCY 7 cut(s) 394, 759, 2308, 2464, 2956, 3032, 3340
PsyI GACNNNGTC 1 cut(s) 1109
PvuII CAGCTG 3 cut(s) 2483, 3374, 5129
RseI CAYNNNNRTG 7 cut(s) 185, 1550, 1691, 1697, 4418, 4682, 4916
SacII CCGCGG 2 cut(s) 1094, 3840
SapI GCTCTTC 2 cut(s) 4, 2940
ScaI AGTACT 3 cut(s) 533, 2736, 2813
SfcI CTRYAG 9 cut(s) 1037, 2436, 2703, 2715, 3917, 4005, 4023, 5193, 5543
SfoI GGCGCC 3 cut(s) 129, 792, 1519
Sfr274I CTCGAG 2 cut(s) 431, 1408
Sfr303I CCGCGG 2 cut(s) 1094, 3840
SfuI TTCGAA 2 cut(s) 1125, 1501
SgrBI CCGCGG 2 cut(s) 1094, 3840
SinI GGWCC 4 cut(s) 157, 691, 1490, 3841
SlaI CTCGAG 2 cut(s) 431, 1408
SmaI CCCGGG 1 cut(s) 635
SmiMI CAYNNNNRTG 7 cut(s) 185, 1550, 1691, 1697, 4418, 4682, 4916
SmlI CTYRAG 8 cut(s) 431, 617, 1408, 1776, 4547, 5240, 5258, 5492
SmoI CTYRAG 8 cut(s) 431, 617, 1408, 1776, 4547, 5240, 5258, 5492
SnaBI TACGTA 1 cut(s) 209
SphI GCATGC 1 cut(s) 3257
SspDI GGCGCC 3 cut(s) 127, 790, 1517
SspI AATATT 1 cut(s) 1445
SspMI CTAG 6 cut(s) 1575, 2423, 2918, 2951, 3290, 4538
StyI CCWWGG 5 cut(s) 93, 1521, 4182, 4347, 5355
TaqII GACCGA 1 cut(s) 1478
TatI WGTACW 9 cut(s) 531, 583, 1635, 2734, 2811, 2967, 3787, 4532, 5559
TscAI CASTG 6 cut(s) 241, 653, 1934, 2551, 4363, 4859
TseFI GTSAC 9 cut(s) 236, 868, 1615, 1882, 1906, 2868, 3292, 3409, 4397
Tsp45I GTSAC 9 cut(s) 236, 868, 1615, 1882, 1906, 2868, 3292, 3409, 4397
TspGWI ACGGA 2 cut(s) 379, 2729
TspMI CCCGGG 1 cut(s) 633
TspRI CASTG 6 cut(s) 241, 653, 1934, 2551, 4363, 4859
Tth111I GACNNNGTC 1 cut(s) 1109
Van91I CCANNNNNTGG 2 cut(s) 4258, 4448
VneI GTGCAC 4 cut(s) 626, 850, 4716, 5006
VpaK11BI GGWCC 4 cut(s) 157, 691, 1490, 3841
VspI ATTAAT 2 cut(s) 1587, 3495
XapI RAATTY 8 cut(s) 271, 1229, 2762, 3193, 3889, 4640, 4656, 4892
XcmI CCANNNNNNNNNTGG 2 cut(s) 71, 4255
XhoI CTCGAG 2 cut(s) 431, 1408
XmaI CCCGGG 1 cut(s) 633
XmiI GTMKAC 2 cut(s) 1923, 4353
XspI CTAG 6 cut(s) 1575, 2423, 2918, 2951, 3290, 4538
ZraI GACGTC 2 cut(s) 90, 241
ZrmI AGTACT 3 cut(s) 533, 2736, 2813
Zsp2I ATGCAT 2 cut(s) 186, 4375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.