MD07G1223800.v1.1

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002740
Physical Location & Seq
Forward (+)
30062151 .. 30073118
10968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1223800.v1.1.491

Sequence Viewer

Length: 5118 bp
ATGGCGGCCGCGAACGCTCCGATCACCATGAAGGAGGCCCTAACGCTTCCGAGCGTTGGGATTAATCCGCAATTCATAACGTTTACACATGTTACTATGGAATCCGATAAGTACATATGCGTTCGGGAGACTTCGCCTCAGAACAGTATTGTTATTATCGATATGAGTATGCCGATGCAACCTCTGAGGAGGCCTATCACTGCGGACTCTGCGCTTATGAATCCGAATTCCAAAATTCTTGCCTTGAAAGCTGGAGTCCAAGGTACTACTCAGGATCACCTTCAAATATTTAACATCGAGATGAAAGCAAAACTGAAATCACATCTTATGCCGGAGCAGATTGTTTTCTGGAAGTGGATAACCCCAAAGATGTTAGGTTTAGTCACTCAGACTACAGTATACCACTGGTCAATTGAAGGTGAATCTGAACCTGTAAAGGTGTTTGAGCGAACAGCTAATCTGGCTAACAATCAGATTATAAATTACCGCTGTGATCCTTCCGAGAAGTGGTTGGTTTTGATTGGAATTGCTCCTGGTGCACCAGAGAGGCCACAATTGGTGAAAGGAAATTTGCAACTCTTCTCTGTGGATCAGCAGCGCAGTCAAGCTCTTGAAGCACATGCTGCATCATTTGCCCAATACAAGGTTCCAGGGAATGAGAATCCTTCAACTCTTATTTCTTTTGCCACTAAGACCCTCAATGCTGGACAGATTACATCAAAGTTGCATGTTATTGAGCTCGGTGCCCAGCCAGGGAAACCATCTTTTACGAAGAAACAAGCAGATCTTTTCTTTCCTCCAGATTTTAATGATGATTTCCCAGTCGCCATGCAGATGTCTCACAAGTACAGCTTGATTTATGTAATTACAAAGCTTGGGCTACTATTTGTGTATGATTTAGAGACAGCTAGTGCCGTTTACAGAAATAGAATTAGTCCGGATCCAATTTTCTTGACAACGGAGGCTTCATCTGTTGGAGGGTTTTATGCGGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACAATTAATGAGCAAACAATTGTGCCTTTTGTCAGCGGCCAGCTAAACAATTTGGAGCTTGCTGTCAATCTGGCTAAAAGAGGAAATCTTCCAGGTGCTGAGAATCTGGTTGTCCAGCGTTTTCAAGAACTGTTTGCCCAAACAAAGTACAAAGAAGCTGCTGAGCTTGCTGCGGAGTCCCCACAAGGAATTCTCCGCACACCTGATACTGTTGCTAAATTTCAGAGTGTTCCTGTCCAAGCTGGGCAAACACCACCTTTGTTGCAGTACTTTGGGACACTCTTGACAAGAGGGAAGCTCAATGCATTTGAATCATTGGAATTGTCACGTCTTGTAGTGAACCAGAATAAGAAGAACCTTTTGGAGAACTGGTTGGCCGAGGACAAACTCGAATGCAGTGAGGAACTGGGAGATCTTGTGAAGACTGTGGATAATGATCTGGCTTTGAAAATATATATTAAAGCCAGAGCAACTCCCAAAGTTGTTGCAGCTTTTGCTGAGCGAAGGGAGTTTGACAAAATACTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTATCTGTTCCTTTTGCAAACAATTCTTCGAGCAGATCCTCAGGGTGCTGTTAATTTTGCATTAATGATGTCTCAAATGGAAGGTGGTTGTCCAGTTGATTACAACACTATAACAGATCTCTTCCTTCAGAGGAACCTTATTCGTGAGGCAACCGCCTTTCTATTGGATGTTTTAAAGCCAAATCTTCCAGAGCATGCATTCCTGCAAACAAAGGTCCTTGAAATCAATTTAGTGACCTTTCCTAATGTGGCTGATGCTATTTTAGCCAATGGAATGTTCAGTCATTATGACCGTCCTCGTATTGCCCAGCTATGCGAAAAAGCTGGTCTTTATGTGCGAGCTTTGCAACATTACTCAGAGTTGCCTGATATTAAACGTGTCATAGTGAATACACATGCAATAGAGCCACAGTCACTTGTGGAGTTTTTTGGCACTCTGTCGAAAGAATGGGCATTGGAGTGCATGAAAGATCTTTTATTGGTCAACTTAAGAGGAAACCTTCAGATCATTGTGCAGGTTGCTAAGGAATATTCTGAGCAGTTGGGTGTTGATCAATGCATGAAGCTCTTTGAACAGTTCAAGTCATATGAAGGGTTGTACTTCTTCCTTGGTTCGTTTTTGAGCTCAAGTGAGGATCCTGATATCCACTTCAAGTACATTGAGGCAGCTGCTAAAACTGGACAAATCAAGGAGGTGGAGCGAGTTACAAGAGAATCAAACTTCTATGATGCTGAGAAGACAAAGAACTTCTTAATGGAAGCTAAGCTCCCCGATGCGCGTCCATTAATTAATGTTTGCGATCGTTTTGGATTTGTTCCAGATCTCACCCACTACTTGTACACGAACAATATGCTTCGTTACATTGAAGGTTACGTTCAAAAGGTGAACCCTGGGAATGCTCCATTAGTTGTGGGACAGTTGTTAGATGATGAGTGCCCTGAAGATTTTATCAAAGGCTTGATTCTTTCTGTTCGTTCATTGCTTCCAGTGGAGCCCCTTGTGGAGGAATGTGAGAAGAGGAATCGCCTTCGTTTACTCACACAGTTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGATGTACATGTGCACAATGCTTTGGGTAAAATCATCATTGATAGTGGCAATAATCCAGAGCACTTCCTTACCACAAACCCATATTATGATTCACGTGTTGTTGGTAAATATTGTGAGAAACGTGATCCTACCCTAGCTGTTGTTGCTTACCGCCGTGGACAATGTGACGATGAACTTATTAATGTCACAAATAAGAACTCATTGTTCAAATTGCAAGCAAGGTATGTGGTTGAGAGGATGGATGAAGATCTTTGGGGGAAAGTTCTTGACCCTGAGAATGAGTACAGAAGACAGCTTATTGATCAAGTTGTTTCTACTGCCTTGCCCGAGAGCAAGAGCCCGGAGCAAGTTTCTGCGTCTGTTAAGGCTTTCATGACTGCTGATTTGCCTCACGAATTAATTGAGCTTCTTGAAAAGATTGTGCTTCAAAACTCTGCCTTCAGTGGAAACTTTAATCTGCAAAACCTGCTAATTTTGACAGCCATCAAGGCAGATCCGTCTAGAGTTATGGATTATATTAATAGGCTAGATAATTTTGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCTCAATTGTATGAGGAAGCATTTGCAATCTTCAAGAAGTTTAACTTGAATGTCCAAGCTGTTAATGTCTTGTTGGATAACATCCGAAGCATTGATAGGGCTGTGGAGTTTGCCTTCCGTGTGGAGGAAGATGCTGTTTGGAGCCAGGTTGCAAAGGCTCAACTTCGGGAGGGTCTAGTGAGCGATGCTATTGAATCATTTATCCGTGCGGATGATGCCACCCAATTCTTGGATGTTATTCGTGCTTCTGAGGATGCAGATGTGTACCATGACTTGGTGAAGTACCTTCTAATGGTTAGGCAGAAGGTCAGAGAACCCAAGGTGGACAGTGAGCTCATTTATGCATATGCAAAGATTGATAGGCTTGCTGACATCGAAGAATTTATTCTTATGCCAAATGTCGCTAATCTCCAAAATGTTGGTGATCGCCTATATGATGAGGCTCTCTATGAAGCTGCAAAGATAATATATGCCTTCATATCTAACTGGGCCAAGTTGGCTGTCACACTTGTGAAGCTCAAACAATTTCAAGGTGCTGTTGATGCAGCACGAAAAGCTAACAGTTCAAAGACATGGAAGGAGGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGCTTAGCTCAGATTTGTGGTCTCAACATCATTATACAGGTGGATGATTTGGAAGAGGTCAGTGAATACTACCAGAATAGAGGATGCTTCAATGAGCTTATATCTCTAATGGAGAGTGGTCTTGGGTTAGAGCGTGCTCACATGGGAATCTTTACTGAGTTGGGGGTTCTATATGCTAGATATCGTCCAGAGAAACTTATGGAGCACATCAAATTGTTCGCAAATCGTCTAAATATCCCCAAGCTTATAAGAGCATGTGATGAGCAGCAACATTGGAAGGAGCTGACCTATCTGTATATTCAATATGATGAGTTTGATAATGCTGCAACTACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAGTTCAAAGATGTGGCAGTTAAAGTTGCTAACGTGGAGCTATATTACAAGGCTGTGCACTTCTACTTGAAAGAGCACCCGGATCTCATTAACGATCTTCTAAATGTGCTCGCGCTTCGTGTAGATCACACACGTGTTGTTGACATCATGCGGAAGGCTGGTCACCTGCTTCTTGTGAAGCCATACATGGTTGCAGTTCAGAGCAACAATGTCTCTGCTGTGAACGAGGCTTTGAATGAGATTTATGTTGAGGAGGAAGACTATGAAAGATTGCGCGAATCAATTGATTTGCATGATAGCTTTGACCAAATAGGCCTCGCTCAAAAGATTGAGAAACATGAGCTTCTGGAAATGAGACGCGTTGCTGCTTATATTTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGGATTGTCAAAGAAAGACAAACTTTACAAAGATGCCATGGAGACAGCCTCACAATCTGGTGACCGTGAACTGGCCGAGGAGTTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCATCGTGCCTCTTTGTCTGTTACGATTTGATAAGGCCGGACATTGCCCTTGAACTTGCATGGATGAACAACATGATCGATTTTGCTTTCCCATATCTGTTACAGTTTATCCGTGAATACACTGGCAAAGTTGATGAACTGGTTAAAGACAAAATTGAAGCACAAAAGGAGATCAAGGCTAAAGAACAAGAAGATAAAGAAGTTATTGCACAGCAGAACATGTATGCTCAGTTGCTGCCTCTTGCTTTGCCTGCACCACCAATCCCTGGTATGAGCGGGGGTATGGGAGGAGGGTATGCTCCACCGCCACCTATGGGAGGAATGCCACCGATGGGCATGCCGCCTTATGGCATGCCACCAATGGGCAGCAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1706

Amino Acids

193.15

Weight (kDa)

5.3

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 4.8e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 9.3e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 4e-28 Clathrin-H-link
Clathrin PF00637 557 - 692 7.9e-25 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 6.4e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 1.3e-32 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 1.5e-31 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 2.3e-30 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1432 1.2e-27 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 8.9e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 479, 4088
AarI CACCTGC 1 cut(s) 4383
Acc36I ACCTGC 5 cut(s) 1549, 2083, 3129, 3208, 4383
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 3 cut(s) 3454, 3499, 5006
AccBSI CCGCTC 1 cut(s) 5016
AccI GTMKAC 1 cut(s) 399
AccII CGCG 5 cut(s) 11, 2356, 4322, 4485, 4569
AccIII TCCGGA 1 cut(s) 937
AclI AACGTT 1 cut(s) 80
AcoI YGGCCR 4 cut(s) 6, 1057, 1395, 4689
AcsI RAATTY 6 cut(s) 226, 234, 568, 1209, 1238, 3605
AcuI CTGAAG 5 cut(s) 1688, 2063, 2538, 3079, 4212
AcvI CACGTG 2 cut(s) 2750, 4343
AflII CTTAAG 1 cut(s) 2065
AflIII ACRYGT 8 cut(s) 88, 1954, 2662, 2749, 4340, 4342, 4567, 4959
AjiI CACGTC 1 cut(s) 1349
AjnI CCWGG 9 cut(s) 532, 649, 751, 1005, 1111, 2467, 3369, 4196, 5005
AjuI GAANNNNNNNTTGG 6 cut(s) 630, 662, 1364, 1396, 1838, 1870
AleI CACNNNNGTG 2 cut(s) 3734, 4341
Alw26I GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
Alw44I GTGCAC 3 cut(s) 537, 2666, 4265
AlwNI CAGNNNCTG 3 cut(s) 1118, 4975, 5115
Ama87I CYCGRG 1 cut(s) 2981
Aor13HI TCCGGA 1 cut(s) 937
ApaLI GTGCAC 3 cut(s) 537, 2666, 4265
ApoI RAATTY 6 cut(s) 226, 234, 568, 1209, 1238, 3605
ArsI GACNNNNNNTTYG 2 cut(s) 3022, 3054
AseI ATTAAT 8 cut(s) 63, 1026, 1640, 2363, 2367, 2835, 3053, 3174
Asp700I GAANNNNTTC 1 cut(s) 3609
AspLEI GCGC 5 cut(s) 214, 600, 2356, 4324, 4485
AspS9I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 3714
AsuC2I CCSGG 2 cut(s) 2996, 4289
AvaI CYCGRG 1 cut(s) 2981
AvaII GGWCC 2 cut(s) 1792, 3197
AxyI CCTNAGG 1 cut(s) 1617
BaeGI GKGCMC 5 cut(s) 541, 748, 2516, 2670, 4269
BamHI GGATCC 2 cut(s) 940, 2212
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 5 cut(s) 741, 2204, 2574, 2996, 3561
BbrPI CACGTG 2 cut(s) 2750, 4343
BbsI GAAGAC 4 cut(s) 1448, 2321, 2950, 4473
BbvCI CCTCAGC 1 cut(s) 3831
BccI CCATC 6 cut(s) 769, 2887, 3146, 3188, 4181, 5065
BceAI ACGGC 2 cut(s) 899, 2793
BcgI CGANNNNNNTGC 4 cut(s) 2410, 2444, 5059, 5093
BciT130I CCWGG 9 cut(s) 534, 651, 753, 1007, 1113, 2469, 3371, 4198, 5007
BclI TGATCA 2 cut(s) 2128, 2956
BcnI CCSGG 2 cut(s) 2996, 4289
BcoDI GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
BfaI CTAG 6 cut(s) 909, 2789, 3156, 3182, 3401, 4017
BfmI CTRYAG 2 cut(s) 393, 3201
BfrI CTTAAG 1 cut(s) 2065
BfuAI ACCTGC 5 cut(s) 1549, 2083, 3129, 3208, 4383
BglI GCCNNNNNGGC 2 cut(s) 3143, 3722
BglII AGATCT 6 cut(s) 784, 1432, 1693, 2047, 2398, 2902
BlpI GCTNAGC 4 cut(s) 1182, 1518, 2340, 3844
BmcAI AGTACT 1 cut(s) 1289
Bme18I GGWCC 2 cut(s) 1792, 3197
BmeT110I CYCGRG 1 cut(s) 2981
BmgBI CACGTC 1 cut(s) 1349
BmgT120I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 3714
BmiI GGNNCC 8 cut(s) 648, 745, 942, 1004, 1712, 2214, 2571, 3368
BmrI ACTGGG 3 cut(s) 815, 1436, 3721
BmuI ACTGGG 3 cut(s) 815, 1436, 3721
BpiI GAAGAC 4 cut(s) 1448, 2321, 2950, 4473
BplI GAGNNNNNCTC 4 cut(s) 1302, 1334, 4649, 4681
BpmI CTGGAG 2 cut(s) 273, 783
Bpu10I CCTNAGC 2 cut(s) 2100, 3831
Bpu1102I GCTNAGC 4 cut(s) 1182, 1518, 2340, 3844
BpuEI CTTGAG 1 cut(s) 2188
BpuMI CCSGG 2 cut(s) 2996, 4289
Bsa29I ATCGAT 2 cut(s) 159, 4818
BsaAI YACGTR 2 cut(s) 2750, 4343
BsaBI GATNNNNATC 2 cut(s) 1455, 2901
BsaI GGTCTC 1 cut(s) 3866
BsaWI WCCGGW 1 cut(s) 937
BsaXI ACNNNNNCTCC 4 cut(s) 1376, 1406, 1422, 1452
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 2 cut(s) 2555, 4782
Bse8I GATNNNNATC 2 cut(s) 1455, 2901
BseAI TCCGGA 1 cut(s) 937
BseBI CCWGG 9 cut(s) 534, 651, 753, 1007, 1113, 2469, 3371, 4198, 5007
BseCI ATCGAT 2 cut(s) 159, 4818
BseJI GATNNNNATC 2 cut(s) 1455, 2901
BseMI GCAATG 2 cut(s) 2555, 4782
BseRI GAGGAG 5 cut(s) 202, 3848, 4475, 4709, 5043
BseSI GKGCMC 5 cut(s) 541, 748, 2516, 2670, 4269
BseX3I CGGCCG 1 cut(s) 6
BseYI CCCAGC 3 cut(s) 747, 1262, 1884
BsgI GTGCAG 2 cut(s) 2111, 4977
Bsh1236I CGCG 5 cut(s) 11, 2356, 4322, 4485, 4569
Bsh1285I CGRYCG 2 cut(s) 9, 2380
BshNI GGYRCC 1 cut(s) 743
BshVI ATCGAT 2 cut(s) 159, 4818
BsiEI CGRYCG 2 cut(s) 9, 2380
BsiHKCI CYCGRG 1 cut(s) 2981
BsiSI CCGG 5 cut(s) 332, 938, 2996, 4289, 4778
BslFI GGGAC 3 cut(s) 1183, 1309, 2505
BsmAI GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
BsmBI CGTCTC 1 cut(s) 4558
BsmFI GGGAC 3 cut(s) 1183, 1309, 2505
BsmI GAATGC 5 cut(s) 1418, 1775, 2479, 4739, 5067
Bso31I GGTCTC 1 cut(s) 3866
BsoBI CYCGRG 1 cut(s) 2981
Bsp13I TCCGGA 1 cut(s) 937
Bsp1407I TGTACA 2 cut(s) 2415, 2659
Bsp1720I GCTNAGC 4 cut(s) 1182, 1518, 2340, 3844
Bsp19I CCATGG 1 cut(s) 4653
BspDI ATCGAT 2 cut(s) 159, 4818
BspEI TCCGGA 1 cut(s) 937
BspFNI CGCG 5 cut(s) 11, 2356, 4322, 4485, 4569
BspHI TCATGA 2 cut(s) 3027, 4176
BspLI GGNNCC 8 cut(s) 648, 745, 942, 1004, 1712, 2214, 2571, 3368
BspMAI CTGCAG 1 cut(s) 3205
BspMI ACCTGC 5 cut(s) 1549, 2083, 3129, 3208, 4383
BspT107I GGYRCC 1 cut(s) 743
BspTI CTTAAG 1 cut(s) 2065
BspTNI GGTCTC 1 cut(s) 3866
BsrBI CCGCTC 1 cut(s) 5016
BsrDI GCAATG 2 cut(s) 2555, 4782
BsrGI TGTACA 2 cut(s) 2415, 2659
BssNAI GTATAC 1 cut(s) 400
BssT1I CCWWGG 4 cut(s) 259, 2185, 3543, 4653
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 9 cut(s) 534, 651, 753, 1007, 1113, 2469, 3371, 4198, 5007
Bst6I CTCTTC 4 cut(s) 584, 1703, 2588, 3888
BstAFI CTTAAG 1 cut(s) 2065
BstAPI GCANNNNNTGC 4 cut(s) 623, 632, 1514, 3121
BstAUI TGTACA 2 cut(s) 2415, 2659
BstBAI YACGTR 2 cut(s) 2750, 4343
BstDSI CCRYGG 2 cut(s) 2809, 4653
BstEII GGTNACC 2 cut(s) 4370, 4676
BstENI CCTNNNNNAGG 2 cut(s) 2579, 5055
BstFNI CGCG 5 cut(s) 11, 2356, 4322, 4485, 4569
BstHHI GCGC 5 cut(s) 214, 600, 2356, 4324, 4485
BstMAI GTCTC 8 cut(s) 122, 843, 896, 1653, 3866, 4426, 4558, 4652
BstMCI CGRYCG 2 cut(s) 9, 2380
BstNI CCWGG 9 cut(s) 534, 651, 753, 1007, 1113, 2469, 3371, 4198, 5007
BstPI GGTNACC 2 cut(s) 4370, 4676
BstSFI CTRYAG 2 cut(s) 393, 3201
BstSLI GKGCMC 5 cut(s) 541, 748, 2516, 2670, 4269
BstUI CGCG 5 cut(s) 11, 2356, 4322, 4485, 4569
BstV2I GAAGAC 4 cut(s) 1448, 2321, 2950, 4473
BstXI CCANNNNNNTGG 1 cut(s) 3644
BstZ17I GTATAC 1 cut(s) 400
BstZI CGGCCG 1 cut(s) 6
Bsu15I ATCGAT 2 cut(s) 159, 4818
Bsu36I CCTNAGG 1 cut(s) 1617
BsuTUI ATCGAT 2 cut(s) 159, 4818
BtgI CCRYGG 2 cut(s) 2809, 4653
BtgZI GCGATG 1 cut(s) 3423
BtrI CACGTC 1 cut(s) 1349
BtsI GCAGTG 2 cut(s) 198, 1423
BtsIMutI CAGTG 8 cut(s) 198, 403, 1423, 2571, 3103, 3559, 3907, 4860
BveI ACCTGC 5 cut(s) 1549, 2083, 3129, 3208, 4383
CaiI CAGNNNCTG 3 cut(s) 1118, 4975, 5115
CciI TCATGA 2 cut(s) 3027, 4176
CciNI GCGGCCGC 1 cut(s) 6
CfoI GCGC 5 cut(s) 214, 600, 2356, 4324, 4485
Cfr13I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 3714
ClaI ATCGAT 2 cut(s) 159, 4818
CseI GACGC 3 cut(s) 2345, 3000, 4575
CspCI CAANNNNNGTGG 4 cut(s) 1263, 1298, 2862, 2897
DraI TTTAAA 1 cut(s) 1752
EaeI YGGCCR 4 cut(s) 6, 1057, 1395, 4689
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 4 cut(s) 584, 1703, 2588, 3888
EarI CTCTTC 4 cut(s) 584, 1703, 2588, 3888
Ecl136II GAGCTC 3 cut(s) 739, 2202, 3559
EclXI CGGCCG 1 cut(s) 6
Eco130I CCWWGG 4 cut(s) 259, 2185, 3543, 4653
Eco147I AGGCCT 2 cut(s) 193, 4524
Eco24I GRGCYC 5 cut(s) 741, 2204, 2574, 2996, 3561
Eco31I GGTCTC 1 cut(s) 3866
Eco32I GATATC 2 cut(s) 2221, 4022
Eco47I GGWCC 2 cut(s) 1792, 3197
Eco52I CGGCCG 1 cut(s) 6
Eco53kI GAGCTC 3 cut(s) 739, 2202, 3559
Eco57I CTGAAG 5 cut(s) 1688, 2063, 2538, 3079, 4212
Eco72I CACGTG 2 cut(s) 2750, 4343
Eco81I CCTNAGG 1 cut(s) 1617
Eco88I CYCGRG 1 cut(s) 2981
Eco91I GGTNACC 2 cut(s) 4370, 4676
EcoICRI GAGCTC 3 cut(s) 739, 2202, 3559
EcoNI CCTNNNNNAGG 2 cut(s) 2579, 5055
EcoO109I RGGNCCY 2 cut(s) 37, 1792
EcoO65I GGTNACC 2 cut(s) 4370, 4676
EcoRI GAATTC 2 cut(s) 226, 1209
EcoRII CCWGG 9 cut(s) 532, 649, 751, 1005, 1111, 2467, 3369, 4196, 5005
EcoRV GATATC 2 cut(s) 2221, 4022
EcoT14I CCWWGG 4 cut(s) 259, 2185, 3543, 4653
EcoT22I ATGCAT 4 cut(s) 1327, 1777, 2138, 3571
EcoT38I GRGCYC 5 cut(s) 741, 2204, 2574, 2996, 3561
ErhI CCWWGG 4 cut(s) 259, 2185, 3543, 4653
Esp3I CGTCTC 1 cut(s) 4558
FaqI GGGAC 3 cut(s) 1183, 1309, 2505
FauI CCCGC 2 cut(s) 993, 5009
FauNDI CATATG 3 cut(s) 116, 2164, 3571
FbaI TGATCA 2 cut(s) 2128, 2956
FblI GTMKAC 1 cut(s) 399
FriOI GRGCYC 5 cut(s) 741, 2204, 2574, 2996, 3561
FspBI CTAG 6 cut(s) 909, 2789, 3156, 3182, 3401, 4017
GlaI GCGC 5 cut(s) 213, 599, 2355, 4323, 4484
GsaI CCCAGC 3 cut(s) 751, 1266, 1888
GsuI CTGGAG 2 cut(s) 273, 783
HapII CCGG 5 cut(s) 332, 938, 2996, 4289, 4778
HgaI GACGC 3 cut(s) 2345, 3000, 4575
HhaI GCGC 5 cut(s) 214, 600, 2356, 4324, 4485
Hin6I GCGC 5 cut(s) 212, 598, 2354, 4322, 4483
HinP1I GCGC 5 cut(s) 212, 598, 2354, 4322, 4483
HincII GTYRAC 2 cut(s) 2062, 4351
HindII GTYRAC 2 cut(s) 2062, 4351
HindIII AAGCTT 2 cut(s) 872, 4082
HpaII CCGG 5 cut(s) 332, 938, 2996, 4289, 4778
HpyCH4IV ACGT 8 cut(s) 80, 1348, 1954, 2451, 2749, 2776, 4242, 4342
HpySE526I ACGT 8 cut(s) 80, 1348, 1954, 2451, 2749, 2776, 4242, 4342
HspAI GCGC 5 cut(s) 212, 598, 2354, 4322, 4483
Kpn2I TCCGGA 1 cut(s) 937
Ksp22I TGATCA 2 cut(s) 2128, 2956
MaeI CTAG 6 cut(s) 909, 2789, 3156, 3182, 3401, 4017
MaeII ACGT 8 cut(s) 80, 1348, 1954, 2451, 2749, 2776, 4242, 4342
MbiI CCGCTC 1 cut(s) 5016
MfeI CAATTG 5 cut(s) 411, 554, 1038, 3230, 4491
MluI ACGCGT 1 cut(s) 4567
MlyI GAGTC 3 cut(s) 200, 264, 1205
MmeI TCCRAC 2 cut(s) 955, 3279
Mph1103I ATGCAT 4 cut(s) 1327, 1777, 2138, 3571
MroI TCCGGA 1 cut(s) 937
MroXI GAANNNNTTC 1 cut(s) 3609
MslI CAYNNNNRTG 5 cut(s) 299, 833, 2035, 3734, 4341
MspA1I CMGCKG 3 cut(s) 489, 1056, 2246
MspCI CTTAAG 1 cut(s) 2065
MspI CCGG 5 cut(s) 332, 938, 2996, 4289, 4778
MunI CAATTG 5 cut(s) 411, 554, 1038, 3230, 4491
Mva1269I GAATGC 5 cut(s) 1418, 1775, 2479, 4739, 5067
MvaI CCWGG 9 cut(s) 534, 651, 753, 1007, 1113, 2469, 3371, 4198, 5007
MvnI CGCG 5 cut(s) 11, 2356, 4322, 4485, 4569
NciI CCSGG 2 cut(s) 2996, 4289
NcoI CCATGG 1 cut(s) 4653
NdeI CATATG 3 cut(s) 116, 2164, 3571
NlaIV GGNNCC 8 cut(s) 648, 745, 942, 1004, 1712, 2214, 2571, 3368
NmeAIII GCCGAG 2 cut(s) 1423, 4717
NmuCI GTSAC 9 cut(s) 382, 1344, 1810, 1989, 2819, 2839, 3727, 4370, 4676
NotI GCGGCCGC 1 cut(s) 6
NsiI ATGCAT 4 cut(s) 1327, 1777, 2138, 3571
OliI CACNNNNGTG 2 cut(s) 3734, 4341
PacI TTAATTAA 1 cut(s) 2367
PaeI GCATGC 3 cut(s) 1775, 5080, 5095
PagI TCATGA 2 cut(s) 3027, 4176
PaqCI CACCTGC 1 cut(s) 4383
PasI CCCWGGG 1 cut(s) 2468
PceI AGGCCT 2 cut(s) 193, 4524
PciI ACATGT 3 cut(s) 88, 2662, 4959
PctI GAATGC 5 cut(s) 1418, 1775, 2479, 4739, 5067
PdmI GAANNNNTTC 1 cut(s) 3609
PflMI CCANNNNNTGG 3 cut(s) 3454, 3499, 5006
Ple19I CGATCG 1 cut(s) 2380
PleI GAGTC 3 cut(s) 200, 263, 1204
PmaCI CACGTG 2 cut(s) 2750, 4343
PmlI CACGTG 2 cut(s) 2750, 4343
PpsI GAGTC 3 cut(s) 200, 263, 1204
Ppu21I YACGTR 2 cut(s) 2750, 4343
PpuMI RGGWCCY 1 cut(s) 1792
PscI ACATGT 3 cut(s) 88, 2662, 4959
PshBI ATTAAT 8 cut(s) 63, 1026, 1640, 2363, 2367, 2835, 3053, 3174
PsiI TTATAA 2 cut(s) 479, 4088
Psp124BI GAGCTC 3 cut(s) 741, 2204, 3561
Psp1406I AACGTT 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 1792
Psp6I CCWGG 9 cut(s) 532, 649, 751, 1005, 1111, 2467, 3369, 4196, 5005
PspCI CACGTG 2 cut(s) 2750, 4343
PspEI GGTNACC 2 cut(s) 4370, 4676
PspFI CCCAGC 3 cut(s) 747, 1262, 1884
PspGI CCWGG 9 cut(s) 532, 649, 751, 1005, 1111, 2467, 3369, 4196, 5005
PspN4I GGNNCC 8 cut(s) 648, 745, 942, 1004, 1712, 2214, 2571, 3368
PspPI GGNCC 5 cut(s) 37, 1003, 1792, 3197, 3714
PspPPI RGGWCCY 1 cut(s) 1792
PstI CTGCAG 1 cut(s) 3205
PstNI CAGNNNCTG 3 cut(s) 1118, 4975, 5115
PvuI CGATCG 1 cut(s) 2380
PvuII CAGCTG 1 cut(s) 2246
RseI CAYNNNNRTG 5 cut(s) 299, 833, 2035, 3734, 4341
SacI GAGCTC 3 cut(s) 741, 2204, 3561
Sau96I GGNCC 5 cut(s) 37, 1003, 1792, 3197, 3714
ScaI AGTACT 1 cut(s) 1289
SchI GAGTC 3 cut(s) 200, 264, 1205
SfcI CTRYAG 2 cut(s) 393, 3201
SinI GGWCC 2 cut(s) 1792, 3197
SmiMI CAYNNNNRTG 5 cut(s) 299, 833, 2035, 3734, 4341
SmlI CTYRAG 2 cut(s) 2065, 2203
SmoI CTYRAG 2 cut(s) 2065, 2203
SphI GCATGC 3 cut(s) 1775, 5080, 5095
SseBI AGGCCT 2 cut(s) 193, 4524
SspI AATATT 3 cut(s) 288, 2108, 2765
SspMI CTAG 6 cut(s) 909, 2789, 3156, 3182, 3401, 4017
SstI GAGCTC 3 cut(s) 741, 2204, 3561
StuI AGGCCT 2 cut(s) 193, 4524
StyI CCWWGG 4 cut(s) 259, 2185, 3543, 4653
TaiI ACGT 8 cut(s) 83, 1351, 1957, 2454, 2752, 2779, 4245, 4345
TaqI TCGA 7 cut(s) 159, 297, 1410, 1606, 2018, 3600, 4818
TatI WGTACW 9 cut(s) 111, 846, 1167, 1287, 2175, 2232, 2415, 2659, 2937
TauI GCSGC 4 cut(s) 8, 11, 1059, 5083
TscAI CASTG 8 cut(s) 205, 410, 1423, 2571, 3103, 3559, 3907, 4867
TseFI GTSAC 9 cut(s) 382, 1344, 1810, 1989, 2819, 2839, 3727, 4370, 4676
Tsp45I GTSAC 9 cut(s) 382, 1344, 1810, 1989, 2819, 2839, 3727, 4370, 4676
TspGWI ACGGA 5 cut(s) 974, 3141, 3332, 3419, 4841
TspRI CASTG 8 cut(s) 205, 410, 1423, 2571, 3103, 3559, 3907, 4867
Van91I CCANNNNNTGG 3 cut(s) 3454, 3499, 5006
Vha464I CTTAAG 1 cut(s) 2065
VneI GTGCAC 3 cut(s) 537, 2666, 4265
VpaK11BI GGWCC 2 cut(s) 1792, 3197
VspI ATTAAT 8 cut(s) 63, 1026, 1640, 2363, 2367, 2835, 3053, 3174
XagI CCTNNNNNAGG 2 cut(s) 2579, 5055
XapI RAATTY 6 cut(s) 226, 234, 568, 1209, 1238, 3605
XbaI TCTAGA 1 cut(s) 3155
XcmI CCANNNNNNNNNTGG 1 cut(s) 3451
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 3609
XspI CTAG 6 cut(s) 909, 2789, 3156, 3182, 3401, 4017
ZrmI AGTACT 1 cut(s) 1289
Zsp2I ATGCAT 4 cut(s) 1327, 1777, 2138, 3571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.