MD11G1070100.v1.1

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002740
Physical Location & Seq
Reverse (-)
5989499 .. 5999706
10208 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1070100.v1.1.491

Sequence Viewer

Length: 5100 bp
ATGGCCGCCGCTAACGCCCCCATGACGATGAAAGAGGCCCTCACGTTGCCTAGTATTGGGATCAATCCCCAATTCATTACGTTTACGCATGTGACTATGGAATCGGATAAGTACATTTGTGTTAGGGAGACATCGCCGCAGAATAGCGTCGTTATCATCGATATGAGCATGCCCATGCAGCCATTGCGTCGACCCATTACCGCCGACTCGGCTTTGATGAATCCCAATTCCAGAATTCTTGCTCTAAAAGCTCAAGTTCAAGGAACAACTCAGGACCATTTACAAATATTTAACATTGAAATGAAAGCAAAGATGAAATCACATCAGATGCCTGAGCAGATTGTCTTTTGGAAGTGGATTACTCCAAAGATGCTGGGTCTGGTCACGCAGACCTCAGTATATCATTGGTTAATTGAAGGTGAATCCGAGCCTACAAAGGTCTTTGAGCGAACAGCTAATTTGGCTAACAATCAAATAATCAACTACCGTTGTGATCCTTCGGAAAAATGGTTGGTCTTAATTGGAATTGCTCCTGGTTCAGCAGAGAGGCCACAACTTGTCAAAGGAAATATGCAGCTTTTTTCTGTGGAACAGAAACGTAGTCAGGCTCTTGAAGCACATGCTGCATCGTTTGCCACATATAAAGTTCCTGGGAATGAGCATCCATCAATTCTTATTAGTTTTGCCTCAAAGTCCTCCAATGCTGGACAGGTTACATCAAAGATGCATGTAATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATACACTAAGAAGCAAGCAGATTTATTCTTTCCTCCGGATTTTGCTGATGACTTTCCAGTATCAATGCAGATATCGGAAAAGTATGGGTTGATATATGTTATCACAAAGCTTGGACTTTTATTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCAGATCCTATATTTTTGACAGCTGAAGCTTCAACCGTTGGGGGTTTTTATGCAGTCAATAGGCGAGGCCAGGTCTTACTTGCCACTGTAAATGAACAAACAATTGTACCTTTTGTTAGTGGTCAGTTGAATAATTTGGGGCTTGCAGTGAGTCTTGCGAAAAGAGGAAACCTTCCTGGTGCTGAGGACCTGGTTGTCCAGCGGTTCCAAGAACTGTTTTCTCAGACAAAGTATAAGGAGGCTACAGAGCTTGCCGCGGAATCTCCAATGGGAATACTCCGTACTCCTGATACTGTTGCTAAATTTCAGAGCGTTCCTGTGCAAGCTGGGCAAACCCCACCGTTATTGCAGTATTTTGGAACTCTTTTAACTAGAGGAAAACTCAATGCTTATGAATCATTGGAACTGTCACGTTTAGTTGTAAATCAGAACAAAAAGAATCTTTTGGAGAATTGGCTAGCAGAGGACAAGCTAGAATGTAGTGAAGAGCTTGGAGATCTTGTGAAGACGGTGGATAACGACCTTGCTTTGAAAATATACATCAAAGCCCGGGCAACTCCCAAAGTTGTTGCAGCATTTGCTGAGAGAAGGGAATTTGACAAGATTTTAATATACTCAAAGCAGGTTGGATATACACCTGATTATTTGTTCCTTCTCCAAACAATTTTGCGGGCAGATCCCCAGGGGGCTGTTAATTTTGCTCTAATGATGTCTCAAATGGAGGGAGGTTGTCCCATTGACTACAATACCATCACCGATCTGTTCCTTCAGAGGAACTTGATCCGTGAGGCAACAGCCTTTTTGTTGGATGTTCTGAAGCCGAATCTACCTGAGCATGGATTCCTTCAGACAAAGGTTTTGGAAATCAATCTTGTGACTTTTCCAAATGTTGCCGATGCTATATTGGCCAATGGTATGTTCAGTCATTATGATCGCCCACGTATTGGTCAACTTTGTGAAAAAGCTGGTTTGTATTTGCGTGCCCTACAGCACTACACTGAGTTGCCTGATATCAAACGTGTCATCGTGAACACACATGCAATTGACCCTCAGGCTCTTGTCGAGTTCTTTGGAACTCTTTCTGTAGAATGGGCTCTTGAGTGCATGAAAGATCTTCTGCTGGTCAATCTTAGGGGCAATCTTCAGATTATTGTACAGACCGCCAAAGAGTATTCCGAGCAACTGGGTGTAGATAAGTGCATAAAATTGTTTGAGCAGTTCAAGTCCTATGAGGGGCTGTACTTCTTCCTAGGCGCATATTTGAGCTCCAGTGAGGATCCTGATATTCATTTCAAGTACATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGTGTGACACGGGAATCCAATTTTTATGACCCTGAAAAAGCTAAGAACTTTTTAATGGAAGCAAAGCTTCCAGACGCACGTCCTCTGATTAATGTCTGTGATCGTTTTGGCTTCGTTCCAGATCTCACCCATTATTTGTATACAAACAATATGCTTCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCAGGAAATGCTCCTTTAGTTGTAGGGCAGCTATTGGATGATGAATGCCCTGAAGATTTCATTAAAGGTCTTATTCTATCAGTTCGTTCTCTTCTACCGGTTGAGCCCCTTGTCGAGGAATGTGAAAAGAGGAATCGTCTTCGCTTGCTCACCCAATTCTTGGAGCATCTTGTGAGTGAGGGAAGCCAAGATGTACATGTCCATAATGCTCTGGGTAAAATCATCATAGATAGCGGCAACAATCCTGAGCATTTTCTCACAACTAACCCATACTATGATTCTCGTGTTGTTGGTAAATATTGTGAAAAAAGGGATCCTACCCTTGCTGTTGTCGCATACCGTAGAGGGCAATGCGATGATGAACTTGTTAACGTTACAAATAAAAATTCTTTGTTCAAACTCCAGGCCAGATATGTTGTGGAAAGAATGGATGATGATCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAATATAGAAGGCAGCTAATCGATCAAGTTGTATCTACTGCCTTGCCTGAAAGCAAGAGTCCTGAGCAAGTATCATCAGCTGTTAAAGCATTTATGACTGCAGACCTCCCACACGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTTCAAAATTCTGCTTTTAGTGGGAACTTCAATTTGCAGAATCTATTGATTCTGACTGCCATCAAAGCTGATCCATCCAGAGTCATGGACTACATCAATAGATTAGACAACTTTGATGGACCTGCAGTTGGAGTAATGGCTGTAGAAGCTCAGTTGTATGAAGAAGCATTTGCAATCTTCAAGAAGTTCAATTTGAATGTTGATGCTGTGAATGTCCTGTTGGACAACATACAGAGCATTGACCGAGCTGTAGAGTTTGCATTCCGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTGGCCAAGGCTCAACTGCGAGAGGGATTAGTGAGTGATGCCATCGAGTCATTCATCCGTGCAGATGATGCCACCCACTTTTTGGAGGTCATACGTGCTTCTGAAGATGCAGATGTATATCATGACTTGGTAAGATACCTTCTGATGGTCAGGCAGAAGACCAAGGAGCCGAAGGTGGACAGTGAACTTATTTATGCTTATGCTAAGATTGATCGGCTTGGTGATATTGAAGAGTTCATTCTGATGCCAAATGTTGCCAATCTCCAAAATGTTGGGGATCGCTTGTTTGATGAAACCCTATACGAAGCAGCCAAGATCATATTTGCATTCATTTCTAACTGGGGTAAACTAGCATGCACTCTTGTGAAGCTAAATCAGTTTCAAGGTGCTGTTGATGCAGCTCGGAAAGCTAACAGCTCAAAGACGTGGAAGTTTGTTTGCTTTGCATGTGTGGATGCTGAGGAATTCCGCTTAGCTCAGATTTGTGGACTCAACATCATTATCCAGGTGGATGATCTGGAAGAGGTCTGTGAGTACTACCAGAACAGGGGATGCTTTAATGAGCTCATTAGTCTTATGGAGAGTGGGCTAGGATTGGAGCGTGCACATATGGGCATCTTTACAGAATTAGGAGTTCTATATGCAAGATACCGTCCTGAGAAGCTTATGGAGCACATTAAATTATTCTCTACCCGTCTCAACATTCCCAAGCTTATTAGAGCTTGTGATGAACAGCAGCACTGGAAAGAATTAACATACTTATATGTCCAATATGATGAGTTTGATAATGCTGCAACTACCATAATGAACCACTCCCCAGAAGCATGGGACCACATGCAATTCAAGGATGTTATTGTCAAAGTTGCGAGTGTCGAACTCTACTATAGGGCTGTACACTTCTACTTGGAAGAACATCCTGATGTTATTAATGATGTTCTTAATGTGATTGCGCTTCGTGTGGATCACACCAGAGTGGTGGATATAATGAGAAAGGCTGGTCACATCTGTCTGATAAAGCCATACATGGTTGCAGTTCAGAGCAACAATGTAACTGCTGTGAATGAAGCTCTAAATGAAATATACATGGAGGAGGAAGACTATGACAGGCTTCGTGAATCAATAGATTTGCACGACAGCTTTGATCAGATAGGTCTTGCTCAAAAGCTTGAGAAACATGAACTTCTTGAGATGAGACGCGTTGCTGCATACATTTACAAGAAAGCTGGCAGATGGAAGCAATCCATTGCATTGTCAAAGAAAGACAATCATTACAAAGATTGCATGGAGACGTGCTCACAGTCTGGTGATCATGAACTTTCGGAGGAACTACTTATCTATTTCATTGAGCAGGGAAAGAAGGAGTGTTTTGCTGCATGCCTATTTGTTTGTTATGATATGATCCGGCCAGATGTTGCTCTTGAGCTTGCGTGGATGAACAATATAATTGATTTTGCCTTACCATACCTCCTGCAGTTTATCCGAGAGTACACAGGTAAGGTGGATGAACTTATCAAGGACAAAATTGAAGCTCAAAACGAGTTGAAAGCCAAAGAGAAAGAAGAGAAGGAACTGGTTGCTCAGCAGAATATGTATGCTCAGTTGCTTCCTGCTTTGCCTGCACCACCGGGAATGGGAGGTTATGCTCCACCACCAATGCCACCAATGGGAGGGATGGGAATGCCGCCAATGCCCCCGTTTGGAATGCCACAAATGGGCCCCTCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1700

Amino Acids

192.71

Weight (kDa)

5.26

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 2.7e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 7.5e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 1.6e-26 Clathrin-H-link
Clathrin PF00637 557 - 692 5e-25 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 7.9e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 2.5e-32 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 8e-32 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 4.1e-29 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1431 6e-28 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 1.9e-29 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1130
Acc36I ACCTGC 2 cut(s) 1549, 3208
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 3 cut(s) 1880, 2626, 3454
AccI GTMKAC 2 cut(s) 190, 2417
AccII CGCG 2 cut(s) 1193, 4569
AccIII TCCGGA 1 cut(s) 799
AclI AACGTT 1 cut(s) 2838
AcoI YGGCCR 4 cut(s) 3, 1842, 3375, 4775
AcsI RAATTY 6 cut(s) 234, 1238, 1529, 2851, 3085, 3836
AcuI CTGAAG 7 cut(s) 981, 1688, 1766, 1772, 2063, 2538, 3495
AflIII ACRYGT 3 cut(s) 1954, 2662, 4567
AgeI ACCGGT 1 cut(s) 2563
AjiI CACGTC 3 cut(s) 2357, 3798, 4662
AjuI GAANNNNNNNTTGG 4 cut(s) 1838, 1870, 3281, 3313
AleI CACNNNNGTG 2 cut(s) 3734, 4343
Alw21I GWGCWC 5 cut(s) 2204, 3939, 3979, 4047, 4667
Alw26I GTCTC 6 cut(s) 122, 896, 1653, 4073, 4558, 4652
Alw44I GTGCAC 1 cut(s) 3975
AlwNI CAGNNNCTG 1 cut(s) 911
Ama87I CYCGRG 1 cut(s) 1485
Aor13HI TCCGGA 1 cut(s) 799
AoxI GGCC 9 cut(s) 3, 36, 548, 1003, 1842, 2871, 3375, 4775, 5086
ApaI GGGCCC 1 cut(s) 5090
ApaLI GTGCAC 1 cut(s) 3975
ApoI RAATTY 6 cut(s) 234, 1238, 1529, 2851, 3085, 3836
ArsI GACNNNNNNTTYG 2 cut(s) 1777, 1809
AseI ATTAAT 2 cut(s) 2367, 4299
AsiGI ACCGGT 1 cut(s) 2563
Asp700I GAANNNNTTC 1 cut(s) 2014
AspA2I CCTAGG 1 cut(s) 2185
AspLEI GCGC 2 cut(s) 2192, 4324
AspS9I GGNCC 8 cut(s) 37, 274, 1123, 2917, 3197, 4201, 5086, 5087
AsuC2I CCSGG 4 cut(s) 1486, 1487, 3344, 4998
AsuHPI GGTGA 6 cut(s) 431, 1681, 2395, 2608, 3605, 4688
AsuNHI GCTAGC 1 cut(s) 1393
AvaI CYCGRG 1 cut(s) 1485
AvaII GGWCC 5 cut(s) 274, 1123, 2917, 3197, 4201
AvrII CCTAGG 1 cut(s) 2185
AxyI CCTNAGG 1 cut(s) 1986
BaeGI GKGCMC 4 cut(s) 748, 1921, 3979, 5090
BaeI ACNNNNGTAYC 2 cut(s) 1026, 1059
BalI TGGCCA 2 cut(s) 1844, 3377
BamHI GGATCC 2 cut(s) 2212, 2779
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 5 cut(s) 2032, 2204, 2574, 3939, 5090
BarI GAAGNNNNNNTAC 2 cut(s) 3495, 3527
BauI CACGAG 2 cut(s) 2748, 3047
BbsI GAAGAC 4 cut(s) 1448, 2597, 3536, 4473
Bbv12I GWGCWC 5 cut(s) 2204, 3939, 3979, 4047, 4667
BbvCI CCTCAGC 2 cut(s) 1119, 3831
BclI TGATCA 2 cut(s) 4513, 4678
BcnI CCSGG 4 cut(s) 1486, 1487, 3344, 4998
BcoDI GTCTC 6 cut(s) 122, 896, 1653, 4073, 4558, 4652
BfaI CTAG 8 cut(s) 51, 899, 1308, 1394, 1409, 2186, 3722, 3962
BfuAI ACCTGC 2 cut(s) 1549, 3208
BglI GCCNNNNNGGC 1 cut(s) 209
BglII AGATCT 3 cut(s) 1432, 2047, 2398
BlnI CCTAGG 1 cut(s) 2185
BlpI GCTNAGC 2 cut(s) 3844, 4950
BmcAI AGTACT 1 cut(s) 3908
Bme18I GGWCC 5 cut(s) 274, 1123, 2917, 3197, 4201
BmeT110I CYCGRG 1 cut(s) 1485
BmgBI CACGTC 3 cut(s) 2357, 3798, 4662
BmgT120I GGNCC 8 cut(s) 37, 274, 1123, 2917, 3197, 4201, 5086, 5087
BmiI GGNNCC 9 cut(s) 745, 1142, 2214, 2781, 3368, 3540, 4202, 5088, 5089
BmrI ACTGGG 2 cut(s) 2129, 3721
BmtI GCTAGC 1 cut(s) 1397
BmuI ACTGGG 2 cut(s) 2129, 3721
BoxI GACNNNNGTC 1 cut(s) 2355
BpiI GAAGAC 4 cut(s) 1448, 2597, 3536, 4473
BplI GAGNNNNNCTC 4 cut(s) 1302, 1334, 4649, 4681
BpmI CTGGAG 2 cut(s) 2188, 2852
Bpu10I CCTNAGC 7 cut(s) 333, 1119, 1767, 2712, 2913, 2997, 3831
Bpu1102I GCTNAGC 2 cut(s) 3844, 4950
BpuEI CTTGAG 5 cut(s) 237, 2054, 4559, 4577, 4811
BpuMI CCSGG 4 cut(s) 1486, 1487, 3344, 4998
Bsa29I ATCGAT 2 cut(s) 159, 2955
BsaAI YACGTR 2 cut(s) 1877, 3467
BsaBI GATNNNNATC 2 cut(s) 2901, 3489
BsaJI CCNNGG 8 cut(s) 650, 1191, 1485, 1617, 1618, 2185, 3378, 3534
BsaWI WCCGGW 2 cut(s) 799, 2563
BsaXI ACNNNNNCTCC 4 cut(s) 1422, 1452, 4821, 4851
Bse118I RCCGGY 1 cut(s) 2563
Bse1I ACTGG 7 cut(s) 821, 2124, 2205, 2260, 3716, 4118, 4947
Bse21I CCTNAGG 1 cut(s) 1986
Bse3DI GCAATG 3 cut(s) 182, 2822, 4614
Bse8I GATNNNNATC 2 cut(s) 2901, 3489
BseAI TCCGGA 1 cut(s) 799
BseCI ATCGAT 2 cut(s) 159, 2955
BseDI CCNNGG 8 cut(s) 650, 1191, 1485, 1617, 1618, 2185, 3378, 3534
BseJI GATNNNNATC 2 cut(s) 2901, 3489
BseMI GCAATG 3 cut(s) 182, 2822, 4614
BseNI ACTGG 7 cut(s) 821, 2124, 2205, 2260, 3716, 4118, 4947
BseRI GAGGAG 1 cut(s) 4475
BseSI GKGCMC 4 cut(s) 748, 1921, 3979, 5090
BseYI CCCAGC 3 cut(s) 373, 747, 1262
BsgI GTGCAG 2 cut(s) 3453, 4974
Bsh1236I CGCG 2 cut(s) 1193, 4569
BshFI GGCC 9 cut(s) 5, 38, 550, 1005, 1844, 2873, 3377, 4777, 5088
BshNI GGYRCC 1 cut(s) 743
BshTI ACCGGT 1 cut(s) 2563
BshVI ATCGAT 2 cut(s) 159, 2955
BsiHKAI GWGCWC 5 cut(s) 2204, 3939, 3979, 4047, 4667
BsiHKCI CYCGRG 1 cut(s) 1485
BsiSI CCGG 6 cut(s) 800, 1486, 2564, 3343, 4774, 4997
BslFI GGGAC 2 cut(s) 1653, 4214
BsmAI GTCTC 6 cut(s) 122, 896, 1653, 4073, 4558, 4652
BsmBI CGTCTC 3 cut(s) 4073, 4558, 4652
BsmFI GGGAC 2 cut(s) 1653, 4214
BsmI GAATGC 5 cut(s) 2516, 3338, 3698, 5055, 5079
BsnI GGCC 9 cut(s) 5, 38, 550, 1005, 1844, 2873, 3377, 4777, 5088
BsoBI CYCGRG 1 cut(s) 1485
Bsp120I GGGCCC 1 cut(s) 5086
Bsp13I TCCGGA 1 cut(s) 799
Bsp1407I TGTACA 3 cut(s) 2089, 2659, 4264
Bsp1720I GCTNAGC 2 cut(s) 3844, 4950
BspANI GGCC 9 cut(s) 5, 38, 550, 1005, 1844, 2873, 3377, 4777, 5088
BspDI ATCGAT 2 cut(s) 159, 2955
BspEI TCCGGA 1 cut(s) 799
BspFNI CGCG 2 cut(s) 1193, 4569
BspHI TCATGA 2 cut(s) 3493, 4681
BspLI GGNNCC 9 cut(s) 745, 1142, 2214, 2781, 3368, 3540, 4202, 5088, 5089
BspMAI CTGCAG 4 cut(s) 916, 3037, 3205, 4845
BspMI ACCTGC 2 cut(s) 1549, 3208
BspOI GCTAGC 1 cut(s) 1397
BspQI GCTCTTC 1 cut(s) 1416
BspT107I GGYRCC 1 cut(s) 743
BsrDI GCAATG 3 cut(s) 182, 2822, 4614
BsrFI RCCGGY 1 cut(s) 2563
BsrGI TGTACA 3 cut(s) 2089, 2659, 4264
BsrI ACTGG 7 cut(s) 821, 2124, 2205, 2260, 3716, 4118, 4947
BssAI RCCGGY 1 cut(s) 2563
BssECI CCNNGG 8 cut(s) 650, 1191, 1485, 1617, 1618, 2185, 3378, 3534
BssNAI GTATAC 1 cut(s) 2418
BssSI CACGAG 2 cut(s) 2748, 3047
BssT1I CCWWGG 3 cut(s) 2185, 3378, 3534
Bst1107I GTATAC 1 cut(s) 2418
Bst2BI CACGAG 2 cut(s) 2748, 3047
Bst6I CTCTTC 5 cut(s) 1416, 2562, 3597, 3888, 4926
BstAPI GCANNNNNTGC 5 cut(s) 184, 623, 632, 1514, 3440
BstAUI TGTACA 3 cut(s) 2089, 2659, 4264
BstBAI YACGTR 2 cut(s) 1877, 3467
BstDSI CCRYGG 1 cut(s) 1191
BstENI CCTNNNNNAGG 1 cut(s) 2579
BstFNI CGCG 2 cut(s) 1193, 4569
BstHHI GCGC 2 cut(s) 2192, 4324
BstMAI GTCTC 6 cut(s) 122, 896, 1653, 4073, 4558, 4652
BstPAI GACNNNNGTC 1 cut(s) 2355
BstSLI GKGCMC 4 cut(s) 748, 1921, 3979, 5090
BstUI CGCG 2 cut(s) 1193, 4569
BstV2I GAAGAC 4 cut(s) 1448, 2597, 3536, 4473
BstX2I RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
BstXI CCANNNNNNTGG 4 cut(s) 3163, 3644, 4197, 4348
BstYI RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
BstZ17I GTATAC 1 cut(s) 2418
Bsu15I ATCGAT 2 cut(s) 159, 2955
Bsu36I CCTNAGG 1 cut(s) 1986
BsuRI GGCC 9 cut(s) 5, 38, 550, 1005, 1844, 2873, 3377, 4777, 5088
BsuTUI ATCGAT 2 cut(s) 159, 2955
BtgI CCRYGG 1 cut(s) 1191
BtgZI GCGATG 2 cut(s) 117, 2835
BtrI CACGTC 3 cut(s) 2357, 3798, 4662
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 6 cut(s) 1020, 1089, 1932, 2212, 3559, 4111
BveI ACCTGC 2 cut(s) 1549, 3208
CaiI CAGNNNCTG 1 cut(s) 911
CciI TCATGA 2 cut(s) 3493, 4681
CfoI GCGC 2 cut(s) 2192, 4324
Cfr10I RCCGGY 1 cut(s) 2563
Cfr13I GGNCC 8 cut(s) 37, 274, 1123, 2917, 3197, 4201, 5086, 5087
Cfr42I CCGCGG 1 cut(s) 1194
Cfr9I CCCGGG 1 cut(s) 1485
ClaI ATCGAT 2 cut(s) 159, 2955
CseI GACGC 4 cut(s) 136, 176, 2360, 4575
CsiI ACCWGGT 1 cut(s) 1125
CspAI ACCGGT 1 cut(s) 2563
CspCI CAANNNNNGTGG 4 cut(s) 1263, 1298, 3033, 3068
DrdI GACNNNNNNGTC 1 cut(s) 1130
DseDI GACNNNNNNGTC 1 cut(s) 1130
EaeI YGGCCR 4 cut(s) 3, 1842, 3375, 4775
Eam1104I CTCTTC 5 cut(s) 1416, 2562, 3597, 3888, 4926
EarI CTCTTC 5 cut(s) 1416, 2562, 3597, 3888, 4926
Ecl136II GAGCTC 2 cut(s) 2202, 3937
Eco130I CCWWGG 3 cut(s) 2185, 3378, 3534
Eco24I GRGCYC 5 cut(s) 2032, 2204, 2574, 3939, 5090
Eco32I GATATC 2 cut(s) 837, 1948
Eco47I GGWCC 5 cut(s) 274, 1123, 2917, 3197, 4201
Eco53kI GAGCTC 2 cut(s) 2202, 3937
Eco57I CTGAAG 7 cut(s) 981, 1688, 1766, 1772, 2063, 2538, 3495
Eco81I CCTNAGG 1 cut(s) 1986
Eco88I CYCGRG 1 cut(s) 1485
EcoICRI GAGCTC 2 cut(s) 2202, 3937
EcoNI CCTNNNNNAGG 1 cut(s) 2579
EcoO109I RGGNCCY 4 cut(s) 37, 1123, 2917, 5087
EcoRI GAATTC 2 cut(s) 234, 3836
EcoRV GATATC 2 cut(s) 837, 1948
EcoT14I CCWWGG 3 cut(s) 2185, 3378, 3534
EcoT22I ATGCAT 1 cut(s) 729
EcoT38I GRGCYC 5 cut(s) 2032, 2204, 2574, 3939, 5090
ErhI CCWWGG 3 cut(s) 2185, 3378, 3534
Esp3I CGTCTC 3 cut(s) 4073, 4558, 4652
FalI AAGNNNNNCTT 2 cut(s) 2328, 2360
FaqI GGGAC 2 cut(s) 1653, 4214
FauI CCCGC 1 cut(s) 1599
FauNDI CATATG 1 cut(s) 3981
FbaI TGATCA 2 cut(s) 4513, 4678
FblI GTMKAC 2 cut(s) 190, 2417
FriOI GRGCYC 5 cut(s) 2032, 2204, 2574, 3939, 5090
FspBI CTAG 8 cut(s) 51, 899, 1308, 1394, 1409, 2186, 3722, 3962
GlaI GCGC 2 cut(s) 2191, 4323
GsaI CCCAGC 3 cut(s) 377, 751, 1266
GsuI CTGGAG 2 cut(s) 2188, 2852
HaeIII GGCC 9 cut(s) 5, 38, 550, 1005, 1844, 2873, 3377, 4777, 5088
HapII CCGG 6 cut(s) 800, 1486, 2564, 3343, 4774, 4997
HgaI GACGC 4 cut(s) 136, 176, 2360, 4575
HhaI GCGC 2 cut(s) 2192, 4324
Hin6I GCGC 2 cut(s) 2190, 4322
HinP1I GCGC 2 cut(s) 2190, 4322
HincII GTYRAC 3 cut(s) 191, 1886, 2836
HindII GTYRAC 3 cut(s) 191, 1886, 2836
HindIII AAGCTT 6 cut(s) 872, 963, 2342, 4034, 4082, 4535
HpaI GTTAAC 1 cut(s) 2836
HpaII CCGG 6 cut(s) 800, 1486, 2564, 3343, 4774, 4997
HphI GGTGA 6 cut(s) 431, 1681, 2395, 2608, 3605, 4688
Hpy99I CGWCG 2 cut(s) 152, 192
HspAI GCGC 2 cut(s) 2190, 4322
Kpn2I TCCGGA 1 cut(s) 799
Ksp22I TGATCA 2 cut(s) 4513, 4678
KspAI GTTAAC 1 cut(s) 2836
KspI CCGCGG 1 cut(s) 1194
LguI GCTCTTC 1 cut(s) 1416
MabI ACCWGGT 1 cut(s) 1125
MaeI CTAG 8 cut(s) 51, 899, 1308, 1394, 1409, 2186, 3722, 3962
MfeI CAATTG 2 cut(s) 1038, 1977
MflI RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
MlsI TGGCCA 2 cut(s) 1844, 3377
MluI ACGCGT 1 cut(s) 4567
MluNI TGGCCA 2 cut(s) 1844, 3377
MlyI GAGTC 6 cut(s) 200, 1096, 3001, 3168, 3428, 3855
MmeI TCCRAC 4 cut(s) 1543, 1722, 3187, 3279
Mox20I TGGCCA 2 cut(s) 1844, 3377
Mph1103I ATGCAT 1 cut(s) 729
MroI TCCGGA 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 2014
MscI TGGCCA 2 cut(s) 1844, 3377
MslI CAYNNNNRTG 8 cut(s) 26, 161, 173, 299, 3614, 3734, 4290, 4343
Msp20I TGGCCA 2 cut(s) 1844, 3377
MspA1I CMGCKG 5 cut(s) 959, 1138, 1193, 2246, 3014
MspI CCGG 6 cut(s) 800, 1486, 2564, 3343, 4774, 4997
MunI CAATTG 2 cut(s) 1038, 1977
Mva1269I GAATGC 5 cut(s) 2516, 3338, 3698, 5055, 5079
MvnI CGCG 2 cut(s) 1193, 4569
NciI CCSGG 4 cut(s) 1486, 1487, 3344, 4998
NdeI CATATG 1 cut(s) 3981
NheI GCTAGC 1 cut(s) 1393
NlaIV GGNNCC 9 cut(s) 745, 1142, 2214, 2781, 3368, 3540, 4202, 5088, 5089
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 6 cut(s) 91, 382, 1344, 1810, 2281, 4370
NsiI ATGCAT 1 cut(s) 729
OliI CACNNNNGTG 2 cut(s) 3734, 4343
PaeI GCATGC 3 cut(s) 172, 3729, 4749
PagI TCATGA 2 cut(s) 3493, 4681
PasI CCCWGGG 1 cut(s) 1618
PciI ACATGT 1 cut(s) 2662
PciSI GCTCTTC 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 5 cut(s) 2516, 3338, 3698, 5055, 5079
PdmI GAANNNNTTC 1 cut(s) 2014
PflMI CCANNNNNTGG 3 cut(s) 1880, 2626, 3454
PfoI TCCNGGA 1 cut(s) 2467
PinAI ACCGGT 1 cut(s) 2563
PleI GAGTC 6 cut(s) 200, 1095, 3000, 3167, 3427, 3855
PpsI GAGTC 6 cut(s) 200, 1095, 3000, 3167, 3427, 3855
Ppu21I YACGTR 2 cut(s) 1877, 3467
PpuMI RGGWCCY 2 cut(s) 1123, 2917
PscI ACATGT 1 cut(s) 2662
PshAI GACNNNNGTC 1 cut(s) 2355
PshBI ATTAAT 2 cut(s) 2367, 4299
Psp124BI GAGCTC 2 cut(s) 2204, 3939
Psp1406I AACGTT 1 cut(s) 2838
Psp5II RGGWCCY 2 cut(s) 1123, 2917
PspFI CCCAGC 3 cut(s) 373, 747, 1262
PspN4I GGNNCC 9 cut(s) 745, 1142, 2214, 2781, 3368, 3540, 4202, 5088, 5089
PspOMI GGGCCC 1 cut(s) 5086
PspPI GGNCC 8 cut(s) 37, 274, 1123, 2917, 3197, 4201, 5086, 5087
PspPPI RGGWCCY 2 cut(s) 1123, 2917
PstI CTGCAG 4 cut(s) 916, 3037, 3205, 4845
PstNI CAGNNNCTG 1 cut(s) 911
PsuI RGATCY 7 cut(s) 940, 1432, 1612, 2047, 2212, 2398, 2779
PvuII CAGCTG 3 cut(s) 959, 2246, 3014
RseI CAYNNNNRTG 8 cut(s) 26, 161, 173, 299, 3614, 3734, 4290, 4343
SacI GAGCTC 2 cut(s) 2204, 3939
SacII CCGCGG 1 cut(s) 1194
SalI GTCGAC 1 cut(s) 189
SapI GCTCTTC 1 cut(s) 1416
Sau96I GGNCC 8 cut(s) 37, 274, 1123, 2917, 3197, 4201, 5086, 5087
ScaI AGTACT 1 cut(s) 3908
SchI GAGTC 6 cut(s) 200, 1096, 3001, 3168, 3428, 3855
SexAI ACCWGGT 1 cut(s) 1125
Sfr303I CCGCGG 1 cut(s) 1194
SgrBI CCGCGG 1 cut(s) 1194
SinI GGWCC 5 cut(s) 274, 1123, 2917, 3197, 4201
SmaI CCCGGG 1 cut(s) 1487
SmiMI CAYNNNNRTG 8 cut(s) 26, 161, 173, 299, 3614, 3734, 4290, 4343
SmlI CTYRAG 5 cut(s) 252, 2033, 4538, 4556, 4790
SmoI CTYRAG 5 cut(s) 252, 2033, 4538, 4556, 4790
SphI GCATGC 3 cut(s) 172, 3729, 4749
SrfI GCCCGGGC 1 cut(s) 1487
SspI AATATT 2 cut(s) 288, 2765
SspMI CTAG 8 cut(s) 51, 899, 1308, 1394, 1409, 2186, 3722, 3962
SstI GAGCTC 2 cut(s) 2204, 3939
StyI CCWWGG 3 cut(s) 2185, 3378, 3534
TaqI TCGA 8 cut(s) 159, 190, 928, 1998, 2580, 2955, 3417, 4245
TaqII GACCGA 1 cut(s) 3336
TatI WGTACW 8 cut(s) 111, 2089, 2175, 2232, 2659, 3906, 4264, 4857
TauI GCSGC 6 cut(s) 8, 11, 139, 1193, 2703, 5056
TscAI CASTG 6 cut(s) 1027, 1089, 1939, 2212, 3559, 4118
TseFI GTSAC 6 cut(s) 91, 382, 1344, 1810, 2281, 4370
Tsp45I GTSAC 6 cut(s) 91, 382, 1344, 1810, 2281, 4370
TspGWI ACGGA 3 cut(s) 1205, 1709, 3419
TspMI CCCGGG 1 cut(s) 1485
TspRI CASTG 6 cut(s) 1027, 1089, 1939, 2212, 3559, 4118
Van91I CCANNNNNTGG 3 cut(s) 1880, 2626, 3454
VneI GTGCAC 1 cut(s) 3975
VpaK11BI GGWCC 5 cut(s) 274, 1123, 2917, 3197, 4201
VspI ATTAAT 2 cut(s) 2367, 4299
XagI CCTNNNNNAGG 1 cut(s) 2579
XapI RAATTY 6 cut(s) 234, 1238, 1529, 2851, 3085, 3836
XcmI CCANNNNNNNNNTGG 2 cut(s) 2881, 3451
XmaI CCCGGG 1 cut(s) 1485
XmaJI CCTAGG 1 cut(s) 2185
XmiI GTMKAC 2 cut(s) 190, 2417
XmnI GAANNNNTTC 1 cut(s) 2014
XspI CTAG 8 cut(s) 51, 899, 1308, 1394, 1409, 2186, 3722, 3962
ZrmI AGTACT 1 cut(s) 3908
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.