FvH4_3g39020

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002740
Physical Location & Seq
Forward (+)
33247424 .. 33257904
10481 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39020.t1

Sequence Viewer

Length: 5118 bp
ATGGCCTCCGCCGCTGCCAACGCCCCCATGACCATGAAAGAGCAGCTCACGCTGCCGAGTATTGGTATAAATCCGCAGTTCATTACGTTTACGCATGTGACGATGGAATCGGATAAGTACATTTGCGTCAGGGAGACCTCGCCGCAGAACAGCGTGGTCATCATTGACATGAGCATGCCTATGCAGCCGTTGCGGCGACCTATTACTGCCGATTCGGCTCTGATGAATCCGAATTCCAGGATTCTCGCTCTCAAAGCTCAAGTGCAAGGAACTACTCAGGACCATTTACAGATATTTAATATTGAGCTGAAAACGAAGATGAAATCACATCAGATGCCTGAGCAGATTGTGTTTTGGAAGTGGGTCACTCCTAAGATGCTGGGGATGGTCACAGTGACCTCAGTATATCATTGGTTAATTGAAGGGGATTCTGAGCCAACAAAGGTGTTTGAGCGTACAGCTAATTTAGCAAATAATCAAATTATCAACTACCGTTGCGATCCCTCTGAAAAGTGGTTGGTCTTAATTGGAATTGCACCTGGTTCTTCTGAGAGGCCACAACTGGTTAAAGGAAACATGCAGCTTTTCTCTGTGGAGCAGCAACGTAGTCAGGCTCTTGAAGCACATGCTGCATCATTTGCCACAATTAAAGTTGCAGGGAACGAGAACCCTTCAACTCTTATATGCTTTGCTGCAAAGTCCTCTAACGCCGGACAAGTTACATCAAAGTTGCATGTTATTGAGCTTGGTGCCCAGCCAGGAAAACCATCATATACTAAGAAGCAAGCAGATCTCTTCTTCCCTCCGGATTTCGCTGATGACTTTCCTGTGTCAATGCAGATATCAGAGAAGTATGGGTTGATATATGTAATCACAAAGCTTGGACTTCTGTTTGTATATGACCTAGAGACAGCAACTGCAGTTTATAGAAATCGAATCAGTCCGGATCCTATATTTTTGACTGCTGAAGCTTCATCTGTTGGGGGCTTTTATGCAGTCAATAGGCGTGGTCAGGTGTTACTTGCTTCTGTAAATGAACAAACAATTGTACCTTTTGTTAGTGGTCAGTTAAACAATTTGGGACTCGCAGTTAGTCTTGCCAGAAGAGGAAATCTTCCCGGTGCTGAAGACTTGGTTGTCCAGCGATTCCAAGAATTGTTCTCTCAGACGAAATATAAGGAGGCTGCAGAGCTTGCTGCAGATTCTCCAATGGGAGTACTTCGTACACCTGAAACTGTTACTAAATTTCAGAGTGTTCCTGTGCAAGCTGGGCAAACACCACCGTTGTTGCAGTACTTTGGAACTTTGTTAACTAAAGGGAAACTCAACGCGTTTGAATCATTGGAACTGTCACGTTTGGTTGTAAATCAAAACAAGAAAAATCTATTGGAGAATTGGCTAGCAGAGGACAAGCTTGAATGTAGTGAAGAGCTAGGAGATCTTGTGAAGACAGTGGATAATGACCTTGCTTTGAAAATATACATCAAAGCCAGGGCAACTCCAAAAGTTGTTGCAGCCTTTGCTGAGAGAAGGGAATTTGACAAGATTTTAGTATACTCAAAACAGGTTGGATATACACCTGATTATATGTTCCTTCTGCAAACAATTTTGCGGACAGATGCCCAGGGGGCTGTCAATTTTGCTCTAATGATGTCTCAAATGGAGGGAGGTTGCCCTATTGACTACAATACCATTACTGATCTGTTCCTTCAGAGGAATCTCATTCGCGAGGCAACGGCCTTTTTGTTGGATGTGCTGAAGCCGAATCTACCCGAGCATGGCTTCCTTCAGACAAAGGTTTTGGAAATCAATCTTGTGACTTTTCCTAATGTTGCGGATGCTATATTGGCCAACGGTATGTTCAGTCATTATGACCGCCCCCGCGTTGGTCAACTCTGTGAAAAAGCTGGTTTGTATTTGCGTGCCTTACAGCATTATACCGAGTTGCCTGATATTAAACGAGTCATTGTGAACACACATGCAATTGATCCTCAGGCTCTTGTTGAGTTCTTCGGGACTCTTTCCATAGAATGGGCTCTCGAGTGCATGAAAGATCTTCTACTGGTCAATCTTAGGGGCAATCTTCAGATTATTGTGCAGACTGCCAAAGAGTATTCCGAGCAACTGGGTGTAGATAAATGTATAAAGTTGTTTGAGCAGTTCAAGTCCTATGAGGGGTTGTACTTTTTCTTAGGCTCATACTTGAGCTCCAGTGAGGATCCTGATATCCACTTTAAGTATATTGAAGCAGCTGCAAAAACTGGACAGATTAAGGAGGTGGAGCGAGTGACTCGTGAATCTAACTTTTATGATGCCGAGAAGGCTAAGAACTTTCTAATGGAAGCCAAGCTTCCAGATGCGCGTCCTCTAATTAATGTCTGTGATCGTTTTGGCTTTGTTCCTGATCTCACTCATTACCTATATACGAACAATATGCTCCGTTACATTGAAGGTTATGTCCAAAAGGTCAATCCGGGAAATGCTCCTTTAGTTGTTGGGCAGCTTTTGGATGATGAATGCCCTGAAGATTTCATCAAAGGTCTTATTCTATCAGTTCGTTCTCTTCTACCAGTTGAACCCCTTGTTGATGAATGTGAAAAGAGGAATCGTCTTCGCCTGCTCACTCAGTTCTTGGAGCATCTTGTGAGCGAAGGAAGCCAAGATGTACATGTTCATAATGCTCTTGGTAAAATCATCATAGATAGTGGCAACAATCCGGAGCATTTTCTCACAACTAACCCATATTATGATTCCCGCGTTGTGGGTAAATATTGTGAAAAAAGAGATCCGACCCTTGCTGTTGTTGCTTACCGTAGAGGGCAATGTGATGATGAACTTGTTAATGTAACAAATAAGAATTCATTGTTCAAACTCCAGGCCAGATATGTTGTGGAAAGAATGGATGATGACCTCTGGGCTAAGGTCCTTGATCCTGAAAATGAGTATAGAAGGCAGCTCATTGATCAAGTTGTCTCAACTGCTTTGCCTGAAAGCAAGAGCCCTGAGCAAGTTTCAGCAACTGTTAAAGCGTTTATGACCGCAGACCTTCCACATGAGTTGATTGAGCTTCTTGAAAAGATAGTGCTCCAGAATTCAGCTTTTAGTGGAAACTTCAATCTACAGAATCTTTTAATCTTGACTGCAATCAAGGCTGATCCGTCCAGAGTCATGGATTATATCAATCGATTAGACAACTTTGATGGACCTGCTGTTGGAGTAATGGCTATTGAAGCCCAACTCTATGAGGAAGCTTTTGCAATCTTCAAGAAGTTCAATTTGAATGTTGATGCTGTCAATGTTCTGTTGGATAACATTCACAGCATTGACCGTGCGGTAGAGTTTGCATTCAGGGTTGAAGAAGATGCTGTTTGGAGCCAGGTTGCCAAGGCCCAATTACGAGAGGGGCTTGTGAGTGATGCTATTGAGTCATTCATTCGTGCTGATGATGCCACCCAGTTTTTGGATGTCATACGTGCTGCTCAGGATGCAGATGTGTATCATGACTTGGTAAGGTACCTTCTGATGGTTAGGCAGAAGGCGAAAGAGCCCAAGGTAGACAGTGAACTCATTTATGCATATGCTAAGATTGATCGACTCGGTGATATTGAAGAGTTCATTCTTATGCCAAATGTTGCTAATCTCCCAAATGTTGGGGATCGCCTGTATGATGAAGAACTATATGAAGCAGCCAAGATCATATTTGCATTTATTTCTAACTGGGCCAAGCTGGCGTGTACTCTAGTGAAGCTCAAGCAGTTTCAAGGTGCTGTTGATGCTGCCCGGAAAGCTAACAGCTCAAAAACTTGGAAGGAAGTTTGCTTTGCATGTGTTGATGCTGAGGAATTCCGGTTGGCTCAAATTTGTGGACTCAACATCATCATCCAGGTGGATGATTTGGAAGAAGTCAGTGAGTTTTACCAGAACAGGGGGTGCTTTAATGAACTCATTAGTCTCATGGAGAGTGGACTAGGATTGGAACGTGCACATATGGGCATCTTTACTGAGTTGGGAGTTCTATATGCGAGATACCGTCCTGAGAAGCTCATGGAGCACATTAAACTGTTTTCTACTCGTCTCAACATTCCCAAACTTATACGAGCTTGTGATGAACAGCAGCACTGGAAGGAATTAACATACTTATATATACAATATGATGAATTTGATAATGCTGCAACCACCATAATGAACCACTCCCCAGAAGCATGGGAACACATGCAATTCAAGGATGTGATAGTCAAAGTTGCAAGTGTTGAGCTTTACTATAGGGCTGTGCACTTTTATTTGGAAGAACATCCTGATATTATCAATGATGTTCTAAATGTGCTTGCACTTCGTGTGGACCATACCCGAGTGGTGGATATAATGCGAAAGGCTGGTCACATCCGTCTGGTAAAGCCATACATGGTTGCAGTCCAGAGCAACAATGTAACAGCTGTAAATGAAGCTCTAAATGAAATATATGTTGAGGAGGAAGACTATGACAGACTTCGTGAATCTATTGATTTGCATGATAGCTTTGATCAGATAGGTCTTGCACAAAAGCTTGAGAAACATGAGCTTCTTGAGATTAGACGTGTTGCTGCATACATTTATAAGAAAGCTGGAAGATGGAAGCAATCCATTGCATTGTCAAAGAAGGACAAACATTACAAAGACGCAATGGAGACGTGCTCACAGTCTGGTGATCATGATCTTTCCGAGGAATTGCTTGTCTATTTCATCGAGCAGGGAAAGAAAGAATGTTTTGCATCGTGTCTCTTTGTTTGTTATGATTTAATCCGGCCAGACGTTGCTCTTGAGCTGGCATGGATGAACAATATAATCGATTTTGCCTTCCCATACATCCTACAGTTTATGCGTGAGTACACAGGCAAGGTTGATGAACTTATCAAAGACAAAATTGAGTCTCAAAAAGAGGTGAAAGCCAAAGAGCAAGAGGAGAAGGAATTGGTTGCGAAGCAGAACATGTATGCTCAGTTGCTGCCTGCTTTGCCTGCACCTCCGGGTATGGGTGGAGGTTTTGCTCCACCTCCAATGCCACCAATGCCACAGATGGGTGGGATGGGGATGCCTCCCATGCCTCCTTTTGGGATGCCACAAATGGGCCCTTCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1706

Amino Acids

193.11

Weight (kDa)

5.28

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 22 - 57 2.7e-06 Clathrin propeller repeat
Clathrin-link PF09268 346 - 368 6.2e-08 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 371 - 436 3.4e-27 Clathrin-H-link
Clathrin PF00637 559 - 694 2.1e-24 Region in Clathrin and VPS
Clathrin PF00637 703 - 843 7.2e-27 Region in Clathrin and VPS
Clathrin PF00637 852 - 986 2.3e-32 Region in Clathrin and VPS
Clathrin PF00637 995 - 1133 9.7e-33 Region in Clathrin and VPS
Clathrin PF00637 1146 - 1283 6.8e-30 Region in Clathrin and VPS
Clathrin PF00637 1292 - 1433 2.8e-26 Region in Clathrin and VPS
Clathrin PF00637 1440 - 1581 6.6e-31 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4593
AasI GACNNNNNNGTC 1 cut(s) 1136
Acc36I ACCTGC 1 cut(s) 3214
Acc65I GGTACC 1 cut(s) 3513
AccB1I GGYRCC 2 cut(s) 749, 3513
AccB7I CCANNNNNTGG 3 cut(s) 2031, 3460, 3650
AccI GTMKAC 2 cut(s) 1554, 3555
AccII CGCG 5 cut(s) 1331, 1728, 1884, 2362, 2757
AccIII TCCGGA 3 cut(s) 805, 943, 2716
AcoI YGGCCR 2 cut(s) 1848, 4781
AcsI RAATTY 8 cut(s) 232, 1244, 1535, 2857, 3091, 3842, 3858, 4157
AcuI CTGAAG 7 cut(s) 987, 1146, 1694, 1772, 1778, 2069, 2544
AdeI CACNNNGTG 1 cut(s) 4334
AflIII ACRYGT 4 cut(s) 1329, 2668, 4573, 4965
AjiI CACGTC 2 cut(s) 4574, 4668
AjnI CCWGG 8 cut(s) 236, 538, 757, 1490, 1623, 2874, 3375, 3882
AjuI GAANNNNNNNTTGG 4 cut(s) 1370, 1402, 1844, 1876
Alw21I GWGCWC 6 cut(s) 2210, 3087, 3985, 4053, 4275, 4673
Alw26I GTCTC 9 cut(s) 128, 902, 1659, 2977, 3956, 4079, 4658, 4760, 4911
Alw44I GTGCAC 2 cut(s) 3981, 4271
AlwNI CAGNNNCTG 3 cut(s) 917, 3020, 4981
Ama87I CYCGRG 3 cut(s) 1772, 2039, 4347
Aor13HI TCCGGA 3 cut(s) 805, 943, 2716
AoxI GGCC 9 cut(s) 3, 554, 1737, 1848, 2877, 3387, 3720, 4781, 5104
ApaI GGGCCC 1 cut(s) 5108
ApaLI GTGCAC 2 cut(s) 3981, 4271
ApoI RAATTY 8 cut(s) 232, 1244, 1535, 2857, 3091, 3842, 3858, 4157
ArsI GACNNNNNNTTYG 4 cut(s) 1783, 1815, 4360, 4392
AseI ATTAAT 1 cut(s) 2373
Asp718I GGTACC 1 cut(s) 3513
AspLEI GCGC 1 cut(s) 2362
AspS9I GGNCC 8 cut(s) 280, 2923, 3203, 3388, 3720, 4339, 5104, 5105
AsuC2I CCSGG 4 cut(s) 1119, 2475, 3781, 5004
AsuHPI GGTGA 3 cut(s) 3611, 4694, 4930
AsuNHI GCTAGC 1 cut(s) 1399
AvaI CYCGRG 3 cut(s) 1772, 2039, 4347
AvaII GGWCC 4 cut(s) 280, 2923, 3203, 4339
AxyI CCTNAGG 1 cut(s) 1992
BaeGI GKGCMC 4 cut(s) 754, 3985, 4275, 5108
BaeI ACNNNNGTAYC 2 cut(s) 1032, 1065
BalI TGGCCA 1 cut(s) 1850
BamHI GGATCC 2 cut(s) 946, 2218
BanI GGYRCC 2 cut(s) 749, 3513
BanII GRGCYC 5 cut(s) 2038, 2210, 3002, 3549, 5108
BarI GAAGNNNNNNTAC 2 cut(s) 3501, 3533
BauI CACGAG 1 cut(s) 2292
BbsI GAAGAC 4 cut(s) 1134, 1454, 2603, 4479
Bbv12I GWGCWC 6 cut(s) 2210, 3087, 3985, 4053, 4275, 4673
BbvCI CCTCAGC 1 cut(s) 3837
BccI CCATC 8 cut(s) 97, 379, 775, 3194, 3517, 4602, 5047, 5056
BceAI ACGGC 2 cut(s) 172, 1752
BcgI CGANNNNNNTGC 2 cut(s) 2416, 2450
BciT130I CCWGG 8 cut(s) 238, 540, 759, 1492, 1625, 2876, 3377, 3884
BclI TGATCA 3 cut(s) 2962, 4519, 4684
BcnI CCSGG 4 cut(s) 1119, 2475, 3781, 5004
BcoDI GTCTC 9 cut(s) 128, 902, 1659, 2977, 3956, 4079, 4658, 4760, 4911
BfaI CTAG 5 cut(s) 905, 1400, 1433, 3740, 3968
BfmI CTRYAG 6 cut(s) 918, 1185, 1197, 3119, 4261, 4847
BfuAI ACCTGC 1 cut(s) 3214
BglI GCCNNNNNGGC 5 cut(s) 193, 215, 1628, 2321, 3728
BglII AGATCT 3 cut(s) 790, 1438, 2053
BmcAI AGTACT 2 cut(s) 1218, 1295
Bme18I GGWCC 4 cut(s) 280, 2923, 3203, 4339
BmeT110I CYCGRG 3 cut(s) 1772, 2039, 4347
BmgBI CACGTC 2 cut(s) 4574, 4668
BmgT120I GGNCC 8 cut(s) 280, 2923, 3203, 3388, 3720, 4339, 5104, 5105
BmiI GGNNCC 6 cut(s) 751, 948, 2220, 3374, 3515, 5106
BmrI ACTGGG 3 cut(s) 2135, 3448, 3727
BmtI GCTAGC 1 cut(s) 1403
BmuI ACTGGG 3 cut(s) 2135, 3448, 3727
BpiI GAAGAC 4 cut(s) 1134, 1454, 2603, 4479
BplI GAGNNNNNCTC 2 cut(s) 4655, 4687
BpmI CTGGAG 3 cut(s) 2194, 2858, 3071
Bpu10I CCTNAGC 5 cut(s) 339, 2919, 3003, 3480, 3837
BpuEI CTTGAG 6 cut(s) 243, 2224, 3734, 4565, 4583, 4817
BpuMI CCSGG 4 cut(s) 1119, 2475, 3781, 5004
Bsa29I ATCGAT 2 cut(s) 3184, 4824
BsaAI YACGTR 1 cut(s) 3473
BsaBI GATNNNNATC 2 cut(s) 3495, 4689
BsaI GGTCTC 1 cut(s) 128
BsaJI CCNNGG 6 cut(s) 1491, 1623, 1624, 3384, 3549, 4698
BsaWI WCCGGW 4 cut(s) 805, 943, 2716, 3846
BsaXI ACNNNNNCTCC 4 cut(s) 1428, 1458, 2192, 2222
Bse1I ACTGG 9 cut(s) 567, 2067, 2130, 2211, 2266, 2570, 3454, 3722, 4124
Bse21I CCTNAGG 1 cut(s) 1992
Bse3DI GCAATG 3 cut(s) 2828, 4620, 4665
Bse8I GATNNNNATC 2 cut(s) 3495, 4689
BseAI TCCGGA 3 cut(s) 805, 943, 2716
BseBI CCWGG 8 cut(s) 238, 540, 759, 1492, 1625, 2876, 3377, 3884
BseCI ATCGAT 2 cut(s) 3184, 4824
BseDI CCNNGG 6 cut(s) 1491, 1623, 1624, 3384, 3549, 4698
BseJI GATNNNNATC 2 cut(s) 3495, 4689
BseMI GCAATG 3 cut(s) 2828, 4620, 4665
BseNI ACTGG 9 cut(s) 567, 2067, 2130, 2211, 2266, 2570, 3454, 3722, 4124
BseRI GAGGAG 2 cut(s) 4481, 4952
BseSI GKGCMC 4 cut(s) 754, 3985, 4275, 5108
BseYI CCCAGC 3 cut(s) 379, 753, 1268
BsgI GTGCAG 2 cut(s) 2117, 4980
Bsh1236I CGCG 5 cut(s) 1331, 1728, 1884, 2362, 2757
BshFI GGCC 9 cut(s) 5, 556, 1739, 1850, 2879, 3389, 3722, 4783, 5106
BshNI GGYRCC 2 cut(s) 749, 3513
BshVI ATCGAT 2 cut(s) 3184, 4824
BsiHKAI GWGCWC 6 cut(s) 2210, 3087, 3985, 4053, 4275, 4673
BsiHKCI CYCGRG 3 cut(s) 1772, 2039, 4347
BslFI GGGAC 2 cut(s) 1095, 2029
BsmAI GTCTC 9 cut(s) 128, 902, 1659, 2977, 3956, 4079, 4658, 4760, 4911
BsmBI CGTCTC 2 cut(s) 4079, 4658
BsmFI GGGAC 2 cut(s) 1095, 2029
BsmI GAATGC 2 cut(s) 2522, 3344
BsnI GGCC 9 cut(s) 5, 556, 1739, 1850, 2879, 3389, 3722, 4783, 5106
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 3 cut(s) 1772, 2039, 4347
Bsp120I GGGCCC 1 cut(s) 5104
Bsp13I TCCGGA 3 cut(s) 805, 943, 2716
Bsp1407I TGTACA 1 cut(s) 2665
Bsp68I TCGCGA 1 cut(s) 1728
BspANI GGCC 9 cut(s) 5, 556, 1739, 1850, 2879, 3389, 3722, 4783, 5106
BspDI ATCGAT 2 cut(s) 3184, 4824
BspEI TCCGGA 3 cut(s) 805, 943, 2716
BspFNI CGCG 5 cut(s) 1331, 1728, 1884, 2362, 2757
BspHI TCATGA 2 cut(s) 3499, 4687
BspLI GGNNCC 6 cut(s) 751, 948, 2220, 3374, 3515, 5106
BspMAI CTGCAG 3 cut(s) 922, 1189, 1201
BspMI ACCTGC 1 cut(s) 3214
BspOI GCTAGC 1 cut(s) 1403
BspQI GCTCTTC 1 cut(s) 1422
BspT107I GGYRCC 2 cut(s) 749, 3513
BspTNI GGTCTC 1 cut(s) 128
BsrDI GCAATG 3 cut(s) 2828, 4620, 4665
BsrGI TGTACA 1 cut(s) 2665
BsrI ACTGG 9 cut(s) 567, 2067, 2130, 2211, 2266, 2570, 3454, 3722, 4124
BssECI CCNNGG 6 cut(s) 1491, 1623, 1624, 3384, 3549, 4698
BssNAI GTATAC 1 cut(s) 1555
BssSI CACGAG 1 cut(s) 2292
BssT1I CCWWGG 2 cut(s) 3384, 3549
Bst1107I GTATAC 1 cut(s) 1555
Bst2BI CACGAG 1 cut(s) 2292
Bst2UI CCWGG 8 cut(s) 238, 540, 759, 1492, 1625, 2876, 3377, 3884
Bst6I CTCTTC 5 cut(s) 800, 1099, 1422, 2568, 3603
BstAPI GCANNNNNTGC 5 cut(s) 190, 629, 638, 1193, 1520
BstAUI TGTACA 1 cut(s) 2665
BstBAI YACGTR 1 cut(s) 3473
BstFNI CGCG 5 cut(s) 1331, 1728, 1884, 2362, 2757
BstHHI GCGC 1 cut(s) 2362
BstMAI GTCTC 9 cut(s) 128, 902, 1659, 2977, 3956, 4079, 4658, 4760, 4911
BstNI CCWGG 8 cut(s) 238, 540, 759, 1492, 1625, 2876, 3377, 3884
BstSFI CTRYAG 6 cut(s) 918, 1185, 1197, 3119, 4261, 4847
BstSLI GKGCMC 4 cut(s) 754, 3985, 4275, 5108
BstUI CGCG 5 cut(s) 1331, 1728, 1884, 2362, 2757
BstV2I GAAGAC 4 cut(s) 1134, 1454, 2603, 4479
BstX2I RGATCY 6 cut(s) 790, 946, 1438, 2053, 2218, 2785
BstXI CCANNNNNNTGG 2 cut(s) 3169, 4203
BstYI RGATCY 6 cut(s) 790, 946, 1438, 2053, 2218, 2785
BstZ17I GTATAC 1 cut(s) 1555
Bsu15I ATCGAT 2 cut(s) 3184, 4824
Bsu36I CCTNAGG 1 cut(s) 1992
BsuRI GGCC 9 cut(s) 5, 556, 1739, 1850, 2879, 3389, 3722, 4783, 5106
BsuTUI ATCGAT 2 cut(s) 3184, 4824
BtrI CACGTC 2 cut(s) 4574, 4668
BtsIMutI CAGTG 6 cut(s) 399, 1458, 2218, 3565, 3913, 4117
BtuMI TCGCGA 1 cut(s) 1728
BveI ACCTGC 1 cut(s) 3214
CaiI CAGNNNCTG 3 cut(s) 917, 3020, 4981
CciI TCATGA 2 cut(s) 3499, 4687
CfoI GCGC 1 cut(s) 2362
Cfr13I GGNCC 8 cut(s) 280, 2923, 3203, 3388, 3720, 4339, 5104, 5105
ClaI ATCGAT 2 cut(s) 3184, 4824
CseI GACGC 3 cut(s) 115, 2351, 4664
CsiI ACCWGGT 1 cut(s) 538
CspCI CAANNNNNGTGG 6 cut(s) 988, 1023, 1269, 1304, 3039, 3074
DraIII CACNNNGTG 1 cut(s) 4334
DrdI GACNNNNNNGTC 1 cut(s) 1136
DseDI GACNNNNNNGTC 1 cut(s) 1136
EaeI YGGCCR 2 cut(s) 1848, 4781
Eam1104I CTCTTC 5 cut(s) 800, 1099, 1422, 2568, 3603
EarI CTCTTC 5 cut(s) 800, 1099, 1422, 2568, 3603
Ecl136II GAGCTC 1 cut(s) 2208
Eco130I CCWWGG 2 cut(s) 3384, 3549
Eco24I GRGCYC 5 cut(s) 2038, 2210, 3002, 3549, 5108
Eco31I GGTCTC 1 cut(s) 128
Eco32I GATATC 2 cut(s) 843, 2227
Eco47I GGWCC 4 cut(s) 280, 2923, 3203, 4339
Eco53kI GAGCTC 1 cut(s) 2208
Eco57I CTGAAG 7 cut(s) 987, 1146, 1694, 1772, 1778, 2069, 2544
Eco81I CCTNAGG 1 cut(s) 1992
Eco88I CYCGRG 3 cut(s) 1772, 2039, 4347
EcoICRI GAGCTC 1 cut(s) 2208
EcoO109I RGGNCCY 2 cut(s) 2923, 5105
EcoRI GAATTC 4 cut(s) 232, 2857, 3091, 3842
EcoRII CCWGG 8 cut(s) 236, 538, 757, 1490, 1623, 2874, 3375, 3882
EcoRV GATATC 2 cut(s) 843, 2227
EcoT14I CCWWGG 2 cut(s) 3384, 3549
EcoT22I ATGCAT 1 cut(s) 3577
EcoT38I GRGCYC 5 cut(s) 2038, 2210, 3002, 3549, 5108
ErhI CCWWGG 2 cut(s) 3384, 3549
Esp3I CGTCTC 2 cut(s) 4079, 4658
FalI AAGNNNNNCTT 2 cut(s) 2334, 2366
FaqI GGGAC 2 cut(s) 1095, 2029
FauI CCCGC 2 cut(s) 1889, 2762
FauNDI CATATG 2 cut(s) 3577, 3987
FbaI TGATCA 3 cut(s) 2962, 4519, 4684
FblI GTMKAC 2 cut(s) 1554, 3555
FriOI GRGCYC 5 cut(s) 2038, 2210, 3002, 3549, 5108
FspBI CTAG 5 cut(s) 905, 1400, 1433, 3740, 3968
GlaI GCGC 1 cut(s) 2361
GsaI CCCAGC 3 cut(s) 383, 757, 1272
GsuI CTGGAG 3 cut(s) 2194, 2858, 3071
HaeIII GGCC 9 cut(s) 5, 556, 1739, 1850, 2879, 3389, 3722, 4783, 5106
HgaI GACGC 3 cut(s) 115, 2351, 4664
HhaI GCGC 1 cut(s) 2362
Hin6I GCGC 1 cut(s) 2360
HinP1I GCGC 1 cut(s) 2360
HincII GTYRAC 2 cut(s) 1311, 1892
HindII GTYRAC 2 cut(s) 1311, 1892
HindIII AAGCTT 6 cut(s) 878, 969, 1412, 2348, 3249, 4541
HpaI GTTAAC 1 cut(s) 1311
HphI GGTGA 3 cut(s) 3611, 4694, 4930
HpyCH4IV ACGT 8 cut(s) 86, 604, 1354, 3472, 3979, 4573, 4667, 4788
HpySE526I ACGT 8 cut(s) 86, 604, 1354, 3472, 3979, 4573, 4667, 4788
HspAI GCGC 1 cut(s) 2360
Kpn2I TCCGGA 3 cut(s) 805, 943, 2716
KpnI GGTACC 1 cut(s) 3517
Ksp22I TGATCA 3 cut(s) 2962, 4519, 4684
KspAI GTTAAC 1 cut(s) 1311
LguI GCTCTTC 1 cut(s) 1422
MabI ACCWGGT 1 cut(s) 538
MaeI CTAG 5 cut(s) 905, 1400, 1433, 3740, 3968
MaeII ACGT 8 cut(s) 86, 604, 1354, 3472, 3979, 4573, 4667, 4788
MfeI CAATTG 2 cut(s) 1044, 1983
MflI RGATCY 6 cut(s) 790, 946, 1438, 2053, 2218, 2785
MlsI TGGCCA 1 cut(s) 1850
MluI ACGCGT 1 cut(s) 1329
MluNI TGGCCA 1 cut(s) 1850
MlyI GAGTC 9 cut(s) 1077, 1971, 2011, 2284, 3174, 3434, 3588, 3861, 4913
MmeI TCCRAC 5 cut(s) 1549, 1728, 2813, 3193, 3285
Mox20I TGGCCA 1 cut(s) 1850
Mph1103I ATGCAT 1 cut(s) 3577
MroI TCCGGA 3 cut(s) 805, 943, 2716
MscI TGGCCA 1 cut(s) 1850
MslI CAYNNNNRTG 7 cut(s) 32, 167, 173, 179, 3620, 3884, 4181
Msp20I TGGCCA 1 cut(s) 1850
MspA1I CMGCKG 3 cut(s) 14, 2252, 4433
MunI CAATTG 2 cut(s) 1044, 1983
Mva1269I GAATGC 2 cut(s) 2522, 3344
MvaI CCWGG 8 cut(s) 238, 540, 759, 1492, 1625, 2876, 3377, 3884
MvnI CGCG 5 cut(s) 1331, 1728, 1884, 2362, 2757
NciI CCSGG 4 cut(s) 1119, 2475, 3781, 5004
NdeI CATATG 2 cut(s) 3577, 3987
NheI GCTAGC 1 cut(s) 1399
NlaIV GGNNCC 6 cut(s) 751, 948, 2220, 3374, 3515, 5106
NmeAIII GCCGAG 2 cut(s) 81, 2341
NmuCI GTSAC 8 cut(s) 97, 364, 388, 394, 1350, 1816, 2287, 4376
NruI TCGCGA 1 cut(s) 1728
NsiI ATGCAT 1 cut(s) 3577
PaeI GCATGC 1 cut(s) 178
PaeR7I CTCGAG 1 cut(s) 2039
PagI TCATGA 2 cut(s) 3499, 4687
PasI CCCWGGG 1 cut(s) 1624
PciI ACATGT 2 cut(s) 2668, 4965
PciSI GCTCTTC 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PctI GAATGC 2 cut(s) 2522, 3344
PflMI CCANNNNNTGG 3 cut(s) 2031, 3460, 3650
PfoI TCCNGGA 2 cut(s) 236, 2473
PleI GAGTC 9 cut(s) 1077, 1970, 2011, 2284, 3173, 3433, 3588, 3861, 4912
PpsI GAGTC 9 cut(s) 1077, 1970, 2011, 2284, 3173, 3433, 3588, 3861, 4912
Ppu21I YACGTR 1 cut(s) 3473
PpuMI RGGWCCY 1 cut(s) 2923
PscI ACATGT 2 cut(s) 2668, 4965
PshBI ATTAAT 1 cut(s) 2373
PsiI TTATAA 1 cut(s) 4593
Psp124BI GAGCTC 1 cut(s) 2210
Psp5II RGGWCCY 1 cut(s) 2923
Psp6I CCWGG 8 cut(s) 236, 538, 757, 1490, 1623, 2874, 3375, 3882
PspFI CCCAGC 3 cut(s) 379, 753, 1268
PspGI CCWGG 8 cut(s) 236, 538, 757, 1490, 1623, 2874, 3375, 3882
PspN4I GGNNCC 6 cut(s) 751, 948, 2220, 3374, 3515, 5106
PspOMI GGGCCC 1 cut(s) 5104
PspPI GGNCC 8 cut(s) 280, 2923, 3203, 3388, 3720, 4339, 5104, 5105
PspPPI RGGWCCY 1 cut(s) 2923
PstI CTGCAG 3 cut(s) 922, 1189, 1201
PstNI CAGNNNCTG 3 cut(s) 917, 3020, 4981
PsuI RGATCY 6 cut(s) 790, 946, 1438, 2053, 2218, 2785
PvuII CAGCTG 2 cut(s) 2252, 4433
RruI TCGCGA 1 cut(s) 1728
RseI CAYNNNNRTG 7 cut(s) 32, 167, 173, 179, 3620, 3884, 4181
SacI GAGCTC 1 cut(s) 2210
SapI GCTCTTC 1 cut(s) 1422
Sau96I GGNCC 8 cut(s) 280, 2923, 3203, 3388, 3720, 4339, 5104, 5105
ScaI AGTACT 2 cut(s) 1218, 1295
SchI GAGTC 9 cut(s) 1077, 1971, 2011, 2284, 3174, 3434, 3588, 3861, 4913
SexAI ACCWGGT 1 cut(s) 538
SfcI CTRYAG 6 cut(s) 918, 1185, 1197, 3119, 4261, 4847
Sfr274I CTCGAG 1 cut(s) 2039
SinI GGWCC 4 cut(s) 280, 2923, 3203, 4339
SlaI CTCGAG 1 cut(s) 2039
SmiMI CAYNNNNRTG 7 cut(s) 32, 167, 173, 179, 3620, 3884, 4181
SmlI CTYRAG 7 cut(s) 258, 2039, 2203, 3749, 4544, 4562, 4796
SmoI CTYRAG 7 cut(s) 258, 2039, 2203, 3749, 4544, 4562, 4796
SphI GCATGC 1 cut(s) 178
SspI AATATT 2 cut(s) 301, 2771
SspMI CTAG 5 cut(s) 905, 1400, 1433, 3740, 3968
SstI GAGCTC 1 cut(s) 2210
StyI CCWWGG 2 cut(s) 3384, 3549
TaiI ACGT 8 cut(s) 89, 607, 1357, 3475, 3982, 4576, 4670, 4791
TaqI TCGA 6 cut(s) 934, 2040, 3184, 3592, 4722, 4824
TatI WGTACW 7 cut(s) 117, 1216, 1293, 2181, 2665, 3734, 4863
TauI GCSGC 3 cut(s) 14, 145, 196
TscAI CASTG 6 cut(s) 399, 1458, 2218, 3565, 3913, 4124
TseFI GTSAC 8 cut(s) 97, 364, 388, 394, 1350, 1816, 2287, 4376
Tsp45I GTSAC 8 cut(s) 97, 364, 388, 394, 1350, 1816, 2287, 4376
TspGWI ACGGA 3 cut(s) 2429, 3147, 4373
TspRI CASTG 6 cut(s) 399, 1458, 2218, 3565, 3913, 4124
Van91I CCANNNNNTGG 3 cut(s) 2031, 3460, 3650
VneI GTGCAC 2 cut(s) 3981, 4271
VpaK11BI GGWCC 4 cut(s) 280, 2923, 3203, 4339
VspI ATTAAT 1 cut(s) 2373
XapI RAATTY 8 cut(s) 232, 1244, 1535, 2857, 3091, 3842, 3858, 4157
XcmI CCANNNNNNNNNTGG 2 cut(s) 2887, 3457
XhoI CTCGAG 1 cut(s) 2039
XmiI GTMKAC 2 cut(s) 1554, 3555
XspI CTAG 5 cut(s) 905, 1400, 1433, 3740, 3968
ZrmI AGTACT 2 cut(s) 1218, 1295
Zsp2I ATGCAT 1 cut(s) 3577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.