FvH4_7g24660

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002740
Physical Location & Seq
Forward (+)
19017367 .. 19027913
10547 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24660.t1

Sequence Viewer

Length: 5127 bp
ATGGCGGCGGCTAACGCTCCGATCACCATGAAGGAGGCCTTGACGCTTCCGAGCGTCGGGATTAATCCGCAATTCATAACGTTTACACATGTTACTATGGAGTCAGATAAGTACATATGTGTAAGGGAGACAGCGCCGCAGAATAGTATTGTGATTATTGATATGAGCATGCCGAATCAGCCTTTGAGGAGGCCTATCACTGCAGACTCTGCACTTATGAATCCAAACTCCAAAATCCTTGCTTTGAAAGCTCAAGTCCAGGGATCTACTCAGGACCACCTTCAAATTTTTAACATTGAGATGAAAGCAAAACTGAAATCATACCTTATGCCTGAGCAGATCGTCTTTTGGAAATGGATAACCCCAAAGATGTTGGGCCTTGTCACACAGACTACAGTATATCATTGGTCAATTGAAGGTGAGTCTGAACCTGTAAAGGTATTTGAGCGAACAGCTAATTTGGTGAACAATCAGATAATAAATTACCGTTGTGATCCTTCTGAGAAGTGGTTGGTTCTGATTGGAATTGCTCCCGGTGCTCCTGAGAGGCCACAATTGGTTAAAGGGAACTTGCAACTCTTCTCAGTGGAGCAGCAGCGCAGTCAAGCTCTTGAAGCGCATGCTGCATCCTTCGCCCAGTACAAGGTTCCAGGGAATGAGAACCCTTCCATTCTGATTTCCTTTGCCACCAAGACGCTCAATGCTGGACAGATTACATCCAAGTTGCATGTTATTGAGCTCGGTGCCCAGTCAGGGAAGCCATCTTTTTCAAAGAAACAAGCAGATCTTTTCTTTCCTCCAGATTTTGCTGATGATTTCCCAGTTGCCATGCAGATGTCTCACAAGTACAGTTTAATTTATGTAATTACAAAGCTGGGGCTACTATTTGTATACGACTTAGAGACAGCTAGTGCAGTTTACAGAAATAGAATTAGTCCAGATCCAATTTTCTTGACTACAGAAGCTTCTTCAGTGGGAGGCTTTTATGCGGTTAATAGGCGGGGCCAGGTGTTGTTGGCTACCATTAATGAGCAAACAATTGTGCCTTTTGTCAGTGGTCAGTTAAACAATTTGGAGCTTGCTGTCAATCTGGCAAAAAGAGGAAATCTTCCTGGTGCTGAGAATCTGGTTGTCCAGCGTTTCCAAGAACTGTTTGCCCAGACAAAGTACAAAGAAGCTGCTGAACTGGCTGCTGAATCCCCGCAAGGAATTCTTCGTACACCGGATACAGTTGCTAAATTTCAGAGTGTTCCTGTCCAAGCTGGGCAAACACCTCCTTTGTTGCAGTATTTTGGGACACTCTTAACGAGAGGGAAGCTGAATGCCTTTGAGTCATTGGAATTGTCACGTCTTGTTGTGAACCAGAACAAGAAGAATCTTTTGGAGAACTGGTTGGCAGAGGACAAACTGGAATGCAGTGAGGAACTTGGAGATCTTGTGAAGACTGTGGATAATGATCTGGCTTTGAAGATATACATTAAAGCCCGAGCAACTCCAAAAGTTGTTGCAGCGTTTGCCGAGCGAAGGGAATTTGACAAAATCCTGATCTACTCAAAGCAGGTTGGCTACACACCTGACTATCTGTTCCTTTTGCAAACAATTCTCCGAGCAGACCCTCAGGGTGCTGTTAATTTTGCGTTAATGATGTCCCAAATGGAAGGTGGTTGTCCTGTTGATTACAACACCATTACAGATCTCTTTCTTCAGAGGAATCTGATTCGTGAGGCGACCGCTTTTCTACTGGATGTTTTGAAGCCAAATCTCCCTGAACACGCCTTTTTGCAGACAAAGGTCCTCGAGATTAACTTGGTTACTTTTCCAAACGTGGCTGATGCCATTTTAGCCAATGGTATGTTCAGTCATTATGATCGTCCTCGAATTGCCCAGTTATGTGAAAAAGCTGGTCTTTATGTGCGAGCTCTGCAACACTACTCTGAGTTGCCTGATATTAAACGTGTCATAGTGAATACACATGCAATAGAGCCGCAATCTCTAGTGGAGTTTTTTGGTACTCTGTCAAAGGAATGGGCACTGGAGTGTATGAAGGATCTCTTGCTGGTCAATTTGAGAGGCAACCTTCAGATAATTGTGCAGGTTGCTAAGGAGTACTCTGATCAGTTGGGTACTGATCAATGCATGAAGCTGTTTGAGCAGTTCAAGTCATATGAAGGATTGTACTTCTTCCTCGGATCATTTTTGAGTTCAAGTGAGGATCCTGAAATCCACTTCAAGTACATTGAGGCTGCAGCCAAAACTGGACAAATTAAAGAGGTCGAGAGAGTCACCAGAGAATCAAATTTCTACGATGCTGAGAAAACAAAGAACTTTTTGATGGAAGCTAAGCTCCCAGATGCGCGACCCCTTATCAATGTTTGTGACCGTTTTGGTTTTGTTCCAGATCTCACCCACTACTTGTACACAAACAATATGCTCCGTTACATTGAGGGTTATGTCCAGAAGGTGAATCCTTCAAATGCTCCTCTAGTTGTGGGACAGTTGCTAGATGATGAGTGCCCTGAAGATTTTATCAAAGGCTTGATTCTCTCTGTACGTTCATTGCTTCCAGTGGAGCCCCTTGTGGAGGAATGTGAGAAAAGGAATCGACTTCGTTTACTCACTCAGTTCCTGGAGCATCTTGTGAGTGAGGGAAGCCAGGATGTGCACGTCCACAATGCATTGGGTAAAATCATCATTGATAGTGGAAACAATCCAGAACACTTCCTCACCACGAACCCATATTATGATTCTCGTGTGGTGGGTAAATATTGTGAGAAACGCGATCCTACCCTGGCTGTTGTTGCTTATCGACGTGGACAATGTGATGATGAGCTTATCAATGTTACAAATAAAAACTCTTTGTTCAAGTTACAAGCAAGATATGTGGTTGAGAGGATGGATGAGGATCTTTGGGCTAAAGCTCTTGACCCTGAGAATGAATATAGAAGACAACTTATCGATCAAGTGGTTTCTACTGCCTTGCCCGAAAGCAAGAGTCCAGAACAAGTTTCTGCAGCTGTTAAGGCTTTCATGACAGCTGATTTGCCCCATGAATTAATTGAGCTTCTTGAAAAGATTGTGCTTCAGAACTCTGCCTTCAGTGGGAACTTTAATCTGCAGAATCTGCTAATTTTGACAGCCATCAAGGCAGATCCGTCTAGAGTTATGGATTATATTAATAGGCTAGACAATTTCGATGGACCTGCAGTTGGGGAAGTAGCTGTGGAAGCCCAACTCTTTGAGGAAGCATTTGCAATTTTCAAGAAATTCAACTTGAATGTACAGGCCGTCAATGTCTTGCTTGATAATATCCGAAGCATTGATAGGGCTGTAGAATTTGCATTCCGTGTCGAAGAGGATGCTGTTTGGAGTCAGGTTGGAAAGGCTCAACTCCGGGAGGGGCTTGTGAGCGATGCCATTGAGTCATTTATTCGGGCGGATGATGCCACACAATTCTTGGATGTTATTCGTGCTTCTGAGGATGCAGATGTTTACCATGACTTGGTGAGATACCTTCTGATGGTTAGGCAGAAGACAAAGGAGCCGAGGGTAGACAGTGAGCTTATTTATGCATATGCCAAGATTGATAGGCTAGCTGACATTGAAGAGTTCATTCTTATGCCAAATGTCGCCAATCTCCAAAATGTTGGTGATCGCCTATATGATGAGGCCCTCTATGAGGCTGCAAAAATAATTTATGCTTTTATATCCAACTGGGCCAAGTTGGCTGTCACACTTGTGAAGCTCAAACAATTCCAAGGTGCTGTTGATGCAGCACGAAAAGCCAATAGTGCAAAGACATGGAAGGAAGTTTGCTTTGCTTGTGTTGATGCTGAGGAGTTCCGCTTAGCTCAAATTTGTGGTCTCAACATCATCATACAGGTGGATGATTTGGAGGAGGTCAGTGAATTCTACCAGAATAGAGGATGCTTCAATGAGCTAATATCTCTAATGGAAAGTGGTCTTGGCCTAGAACGTGCACACATGGGAATCTTTACTGAGTTGGGAGTTCTGTATGCTAGATACCGTCCTGAGAAACTAATGGAGCACATCAAACTGTTCTCAACTCGGTTAAATATTCCCAAGCTCATACGAGCTTGTGATGAGCAGCAGCATTGGAAGGAGCTGACCTATTTATACATTCAATATGATGAGTTTGATAATGCTGCAACAACCATCATGAATCATTCCCCTGAAGCCTGGGATCACATGCAGTTCAAAGATGTGGCAGTTAAAGTTGCAAATGTGGAGCTATACTATAAGGCTGTGCACTTTTACTTGCAAGAGCATCCTGATCTCATCAATGATATTCTAAATGTTCTTGCTCTCCGTGTAGATCATACACGTGTGGTGGACATTATGCGAAAGGCTGGTCACCTACTTCTTGTGAAGCCATACATGGTTGCTGTTCAGAGCAACAATGTCTCAGCTGTGAATGAGGCTTTGAATGAGATATATGTTGAAGAGGAAGACTATGAAAGATTGCGTGAATCAATTGATTTACATGACAGCTTTGATCAAATAGGTCTTGCTCAAAAGATCGAAAAACACGAGCTTCTGGAAATGAGGCGTGTTGCTGCTTATATTTACAAGAAAGCTGGAAGGTGGAAGCAGTCCATTGCTTTATCAAAGAAAGATAAACTTTACAAAGATGCCATGGAGACCGCATCGCAGTCTGGTGACCGTGAACTTGCAGAGGAATTGCTTGTTTACTTCATTGAACAGGGAAAGAAAGAATGCTTTGCGTCATGCCTCTTTGTCTGTTACGATTTGATTAGGCCAGATACTGCTCTTGAACTTGCGTGGATGAACAACATGATAGACTTTGCCTTCCCATATCTGTTACAGTTTATCCGTGAATACACCGGTAAAGTGGATGAACTGGTGAAGGACAGAATTGAAGCTCAGAATGAAATGAAGTCCAAGGAGAAGGAAGAGAAAGAAGTTATAGCACAGCAGAACATGTATGCACAACTGTTGCCTCTTGCTTTGCCTGCTCCACCAATGCCCGGTATGGGCGGAGGTATGGGAGGCATGGGCGGAGGGTATGGTCCTCCGCCTCCTATGGGAGGAATGGGGATGCCTCCGATGCCACCTTATGGCATGCCACCAATGGGCAGCAGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 ko04144 ko04721 ko04961 ko05016 ko05100 map04142 map04144 map04721 map04961 map05016 map05100
Pfam Domains
Protein Families
Clathrin Clathrin-link Clathrin_H_link Clathrin_propel

Protein Analysis

1709

Amino Acids

193.44

Weight (kDa)

5.27

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 55 2.9e-06 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 9.3e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 4e-28 Clathrin-H-link
Clathrin PF00637 557 - 692 7.9e-25 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 7.5e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 1.6e-32 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 2.3e-31 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 2.1e-30 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1432 2.2e-27 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 3.7e-32 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1549, 2083, 3208
AccB1I GGYRCC 1 cut(s) 743
AccB7I CCANNNNNTGG 2 cut(s) 3499, 5096
AccI GTMKAC 2 cut(s) 891, 3549
AccII CGCG 2 cut(s) 2356, 2778
AclI AACGTT 1 cut(s) 80
AcsI RAATTY 9 cut(s) 285, 1209, 1238, 1529, 2296, 3263, 3332, 3852, 3905
AcuI CTGAAG 7 cut(s) 954, 1688, 2063, 2538, 3065, 3079, 4212
AcvI CACGTG 1 cut(s) 4343
AflIII ACRYGT 5 cut(s) 88, 1954, 4340, 4342, 4959
AgeI ACCGGT 1 cut(s) 4862
AjiI CACGTC 3 cut(s) 1349, 2665, 2810
AjnI CCWGG 8 cut(s) 258, 649, 1005, 1111, 2625, 2652, 2787, 4196
AjuI GAANNNNNNNTTGG 6 cut(s) 1364, 1396, 1838, 1870, 3945, 3977
AleI CACNNNNGTG 2 cut(s) 2035, 3734
Alw21I GWGCWC 7 cut(s) 541, 741, 1921, 2664, 3979, 4047, 4269
Alw26I GTCTC 6 cut(s) 122, 843, 896, 3866, 4426, 4652
Alw44I GTGCAC 3 cut(s) 2660, 3975, 4265
AlwNI CAGNNNCTG 5 cut(s) 209, 2626, 3121, 4784, 5124
Ama87I CYCGRG 2 cut(s) 1485, 1796
ApaLI GTGCAC 3 cut(s) 2660, 3975, 4265
ApoI RAATTY 9 cut(s) 285, 1209, 1238, 1529, 2296, 3263, 3332, 3852, 3905
ArsI GACNNNNNNTTYG 6 cut(s) 3022, 3054, 3844, 3876, 4354, 4386
AseI ATTAAT 4 cut(s) 63, 1026, 3053, 3174
AsiGI ACCGGT 1 cut(s) 4862
AspLEI GCGC 4 cut(s) 136, 600, 619, 2356
AspS9I GGNCC 8 cut(s) 274, 376, 1003, 1792, 3197, 3667, 3714, 5048
AsuC2I CCSGG 3 cut(s) 534, 3392, 5007
AsuNHI GCTAGC 1 cut(s) 3589
AvaI CYCGRG 2 cut(s) 1485, 1796
AvaII GGWCC 4 cut(s) 274, 1792, 3197, 5048
AxyI CCTNAGG 1 cut(s) 1617
BaeGI GKGCMC 6 cut(s) 748, 2032, 2516, 2664, 3979, 4269
BamHI GGATCC 1 cut(s) 2212
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 3 cut(s) 741, 1921, 2574
BauI CACGAG 2 cut(s) 2748, 4547
BbrPI CACGTG 1 cut(s) 4343
BbsI GAAGAC 4 cut(s) 1448, 2950, 3536, 4473
Bbv12I GWGCWC 7 cut(s) 541, 741, 1921, 2664, 3979, 4047, 4269
BbvCI CCTCAGC 1 cut(s) 3831
BccI CCATC 7 cut(s) 769, 2326, 2887, 3146, 3188, 3511, 4181
BceAI ACGGC 1 cut(s) 3269
BcgI CGANNNNNNTGC 4 cut(s) 3182, 3216, 3338, 3372
BciT130I CCWGG 8 cut(s) 260, 651, 1007, 1113, 2627, 2654, 2789, 4198
BciVI GTATCC 1 cut(s) 1219
BclI TGATCA 3 cut(s) 2113, 2128, 4513
BcnI CCSGG 3 cut(s) 534, 3392, 5007
BcoDI GTCTC 6 cut(s) 122, 843, 896, 3866, 4426, 4652
BfaI CTAG 9 cut(s) 909, 1994, 2483, 2501, 3156, 3182, 3590, 3968, 4017
BfmI CTRYAG 8 cut(s) 201, 393, 957, 2244, 3009, 3113, 3201, 3327
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 3 cut(s) 1549, 2083, 3208
BfuI GTATCC 1 cut(s) 1219
BglI GCCNNNNNGGC 2 cut(s) 3143, 3722
BglII AGATCT 4 cut(s) 784, 1432, 1693, 2398
BlpI GCTNAGC 2 cut(s) 2340, 3844
BmcAI AGTACT 1 cut(s) 2108
Bme18I GGWCC 4 cut(s) 274, 1792, 3197, 5048
BmeT110I CYCGRG 2 cut(s) 1485, 1796
BmgBI CACGTC 3 cut(s) 1349, 2665, 2810
BmgT120I GGNCC 8 cut(s) 274, 376, 1003, 1792, 3197, 3667, 3714, 5048
BmiI GGNNCC 6 cut(s) 648, 745, 1004, 2214, 2571, 3540
BmrI ACTGGG 5 cut(s) 631, 742, 815, 1879, 3721
BmtI GCTAGC 1 cut(s) 3593
BmuI ACTGGG 5 cut(s) 631, 742, 815, 1879, 3721
BoxI GACNNNNGTC 1 cut(s) 1790
BpiI GAAGAC 4 cut(s) 1448, 2950, 3536, 4473
BpmI CTGGAG 3 cut(s) 783, 2054, 2648
Bpu10I CCTNAGC 3 cut(s) 333, 2100, 3831
Bpu1102I GCTNAGC 2 cut(s) 2340, 3844
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 3 cut(s) 534, 3392, 5007
Bsa29I ATCGAT 1 cut(s) 2955
BsaAI YACGTR 1 cut(s) 4343
BsaBI GATNNNNATC 2 cut(s) 1455, 2901
BsaI GGTCTC 2 cut(s) 3866, 4652
BsaJI CCNNGG 9 cut(s) 259, 650, 2185, 2787, 3542, 3754, 4197, 4653, 4920
BsaWI WCCGGW 2 cut(s) 1222, 4862
BsaXI ACNNNNNCTCC 4 cut(s) 1376, 1406, 1422, 1452
Bse118I RCCGGY 1 cut(s) 4862
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 2 cut(s) 2555, 4614
Bse8I GATNNNNATC 2 cut(s) 1455, 2901
BseBI CCWGG 8 cut(s) 260, 651, 1007, 1113, 2627, 2654, 2789, 4198
BseCI ATCGAT 1 cut(s) 2955
BseDI CCNNGG 9 cut(s) 259, 650, 2185, 2787, 3542, 3754, 4197, 4653, 4920
BseJI GATNNNNATC 2 cut(s) 1455, 2901
BseMI GCAATG 2 cut(s) 2555, 4614
BseRI GAGGAG 4 cut(s) 202, 2469, 3848, 3908
BseSI GKGCMC 6 cut(s) 748, 2032, 2516, 2664, 3979, 4269
BseYI CCCAGC 2 cut(s) 874, 1262
BsgI GTGCAG 3 cut(s) 195, 933, 2111
Bsh1236I CGCG 2 cut(s) 2356, 2778
Bsh1285I CGRYCG 1 cut(s) 1731
BshNI GGYRCC 1 cut(s) 743
BshTI ACCGGT 1 cut(s) 4862
BshVI ATCGAT 1 cut(s) 2955
BsiEI CGRYCG 1 cut(s) 1731
BsiHKAI GWGCWC 7 cut(s) 541, 741, 1921, 2664, 3979, 4047, 4269
BsiHKCI CYCGRG 2 cut(s) 1485, 1796
BsiSI CCGG 5 cut(s) 534, 1223, 3391, 4863, 5007
BslFI GGGAC 3 cut(s) 1309, 1633, 2505
BsmAI GTCTC 6 cut(s) 122, 843, 896, 3866, 4426, 4652
BsmFI GGGAC 3 cut(s) 1309, 1633, 2505
BsmI GAATGC 4 cut(s) 1327, 1418, 3338, 4739
Bso31I GGTCTC 2 cut(s) 3866, 4652
BsoBI CYCGRG 2 cut(s) 1485, 1796
Bsp1407I TGTACA 2 cut(s) 2415, 3277
Bsp1720I GCTNAGC 2 cut(s) 2340, 3844
Bsp19I CCATGG 1 cut(s) 4653
BspDI ATCGAT 1 cut(s) 2955
BspFNI CGCG 2 cut(s) 2356, 2778
BspHI TCATGA 2 cut(s) 3027, 4176
BspLI GGNNCC 6 cut(s) 648, 745, 1004, 2214, 2571, 3540
BspMAI CTGCAG 5 cut(s) 205, 2248, 3013, 3117, 3205
BspMI ACCTGC 3 cut(s) 1549, 2083, 3208
BspOI GCTAGC 1 cut(s) 3593
BspT107I GGYRCC 1 cut(s) 743
BspTNI GGTCTC 2 cut(s) 3866, 4652
BsrDI GCAATG 2 cut(s) 2555, 4614
BsrFI RCCGGY 1 cut(s) 4862
BsrGI TGTACA 2 cut(s) 2415, 3277
BssAI RCCGGY 1 cut(s) 4862
BssECI CCNNGG 9 cut(s) 259, 650, 2185, 2787, 3542, 3754, 4197, 4653, 4920
BssNAI GTATAC 1 cut(s) 892
BssSI CACGAG 2 cut(s) 2748, 4547
BssT1I CCWWGG 3 cut(s) 3754, 4653, 4920
Bst1107I GTATAC 1 cut(s) 892
Bst2BI CACGAG 2 cut(s) 2748, 4547
Bst2UI CCWGG 8 cut(s) 260, 651, 1007, 1113, 2627, 2654, 2789, 4198
Bst6I CTCTTC 5 cut(s) 584, 3345, 3597, 4455, 4926
BstAPI GCANNNNNTGC 3 cut(s) 209, 1514, 3121
BstAUI TGTACA 2 cut(s) 2415, 3277
BstBAI YACGTR 1 cut(s) 4343
BstC8I GCNNGC 7 cut(s) 170, 621, 1080, 1917, 3591, 4992, 5102
BstDSI CCRYGG 1 cut(s) 4653
BstEII GGTNACC 2 cut(s) 4370, 4676
BstENI CCTNNNNNAGG 3 cut(s) 2579, 3674, 5064
BstFNI CGCG 2 cut(s) 2356, 2778
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 4 cut(s) 136, 600, 619, 2356
BstMAI GTCTC 6 cut(s) 122, 843, 896, 3866, 4426, 4652
BstMCI CGRYCG 1 cut(s) 1731
BstNI CCWGG 8 cut(s) 260, 651, 1007, 1113, 2627, 2654, 2789, 4198
BstNSI RCATGY 8 cut(s) 92, 172, 623, 731, 1976, 4210, 4963, 5104
BstPAI GACNNNNGTC 1 cut(s) 1790
BstPI GGTNACC 2 cut(s) 4370, 4676
BstSFI CTRYAG 8 cut(s) 201, 393, 957, 2244, 3009, 3113, 3201, 3327
BstSLI GKGCMC 6 cut(s) 748, 2032, 2516, 2664, 3979, 4269
BstUI CGCG 2 cut(s) 2356, 2778
BstV2I GAAGAC 4 cut(s) 1448, 2950, 3536, 4473
BstXI CCANNNNNNTGG 1 cut(s) 3644
BstZ17I GTATAC 1 cut(s) 892
Bsu15I ATCGAT 1 cut(s) 2955
Bsu36I CCTNAGG 1 cut(s) 1617
BsuI GTATCC 1 cut(s) 1219
BsuTUI ATCGAT 1 cut(s) 2955
BtgI CCRYGG 1 cut(s) 4653
BtgZI GCGATG 2 cut(s) 3423, 4650
BtrI CACGTC 3 cut(s) 1349, 2665, 2810
BtsI GCAGTG 2 cut(s) 198, 1423
BveI ACCTGC 3 cut(s) 1549, 2083, 3208
Cac8I GCNNGC 7 cut(s) 170, 621, 1080, 1917, 3591, 4992, 5102
CaiI CAGNNNCTG 5 cut(s) 209, 2626, 3121, 4784, 5124
CciI TCATGA 2 cut(s) 3027, 4176
CfoI GCGC 4 cut(s) 136, 600, 619, 2356
Cfr10I RCCGGY 1 cut(s) 4862
Cfr13I GGNCC 8 cut(s) 274, 376, 1003, 1792, 3197, 3667, 3714, 5048
ClaI ATCGAT 1 cut(s) 2955
CseI GACGC 4 cut(s) 43, 52, 703, 4731
CspAI ACCGGT 1 cut(s) 4862
CspCI CAANNNNNGTGG 2 cut(s) 2862, 2897
Eam1104I CTCTTC 5 cut(s) 584, 3345, 3597, 4455, 4926
EarI CTCTTC 5 cut(s) 584, 3345, 3597, 4455, 4926
EciI GGCGGA 4 cut(s) 3449, 5031, 5043, 5052
Ecl136II GAGCTC 2 cut(s) 739, 1919
Eco130I CCWWGG 3 cut(s) 3754, 4653, 4920
Eco147I AGGCCT 2 cut(s) 38, 193
Eco24I GRGCYC 3 cut(s) 741, 1921, 2574
Eco31I GGTCTC 2 cut(s) 3866, 4652
Eco47I GGWCC 4 cut(s) 274, 1792, 3197, 5048
Eco53kI GAGCTC 2 cut(s) 739, 1919
Eco57I CTGAAG 7 cut(s) 954, 1688, 2063, 2538, 3065, 3079, 4212
Eco72I CACGTG 1 cut(s) 4343
Eco81I CCTNAGG 1 cut(s) 1617
Eco88I CYCGRG 2 cut(s) 1485, 1796
Eco91I GGTNACC 2 cut(s) 4370, 4676
EcoICRI GAGCTC 2 cut(s) 739, 1919
EcoNI CCTNNNNNAGG 3 cut(s) 2579, 3674, 5064
EcoO109I RGGNCCY 2 cut(s) 1792, 3667
EcoO65I GGTNACC 2 cut(s) 4370, 4676
EcoRI GAATTC 2 cut(s) 1209, 3905
EcoRII CCWGG 8 cut(s) 258, 649, 1005, 1111, 2625, 2652, 2787, 4196
EcoT14I CCWWGG 3 cut(s) 3754, 4653, 4920
EcoT22I ATGCAT 3 cut(s) 2138, 2677, 3571
EcoT38I GRGCYC 3 cut(s) 741, 1921, 2574
ErhI CCWWGG 3 cut(s) 3754, 4653, 4920
FaqI GGGAC 3 cut(s) 1309, 1633, 2505
FauI CCCGC 2 cut(s) 993, 1209
FauNDI CATATG 3 cut(s) 116, 2164, 3571
FbaI TGATCA 3 cut(s) 2113, 2128, 4513
FblI GTMKAC 2 cut(s) 891, 3549
FriOI GRGCYC 3 cut(s) 741, 1921, 2574
FspBI CTAG 9 cut(s) 909, 1994, 2483, 2501, 3156, 3182, 3590, 3968, 4017
GlaI GCGC 4 cut(s) 135, 599, 618, 2355
GsaI CCCAGC 2 cut(s) 878, 1266
GsuI CTGGAG 3 cut(s) 783, 2054, 2648
HaeII RGCGCY 1 cut(s) 137
HapII CCGG 5 cut(s) 534, 1223, 3391, 4863, 5007
HgaI GACGC 4 cut(s) 43, 52, 703, 4731
HhaI GCGC 4 cut(s) 136, 600, 619, 2356
Hin6I GCGC 4 cut(s) 134, 598, 617, 2354
HinP1I GCGC 4 cut(s) 134, 598, 617, 2354
HindIII AAGCTT 1 cut(s) 963
HpaII CCGG 5 cut(s) 534, 1223, 3391, 4863, 5007
Hpy99I CGWCG 2 cut(s) 59, 2811
HpyCH4IV ACGT 9 cut(s) 80, 1348, 1824, 1954, 2551, 2664, 2809, 3973, 4342
HpySE526I ACGT 9 cut(s) 80, 1348, 1824, 1954, 2551, 2664, 2809, 3973, 4342
HspAI GCGC 4 cut(s) 134, 598, 617, 2354
Ksp22I TGATCA 3 cut(s) 2113, 2128, 4513
MaeI CTAG 9 cut(s) 909, 1994, 2483, 2501, 3156, 3182, 3590, 3968, 4017
MaeII ACGT 9 cut(s) 80, 1348, 1824, 1954, 2551, 2664, 2809, 3973, 4342
MfeI CAATTG 4 cut(s) 411, 554, 1038, 4491
MlyI GAGTC 8 cut(s) 110, 200, 431, 1340, 2289, 3001, 3376, 3428
MmeI TCCRAC 2 cut(s) 3355, 3732
Mph1103I ATGCAT 3 cut(s) 2138, 2677, 3571
MslI CAYNNNNRTG 7 cut(s) 299, 833, 2035, 3614, 3734, 3878, 4341
MspA1I CMGCKG 3 cut(s) 3014, 3035, 4427
MspI CCGG 5 cut(s) 534, 1223, 3391, 4863, 5007
MunI CAATTG 4 cut(s) 411, 554, 1038, 4491
Mva1269I GAATGC 4 cut(s) 1327, 1418, 3338, 4739
MvaI CCWGG 8 cut(s) 260, 651, 1007, 1113, 2627, 2654, 2789, 4198
MvnI CGCG 2 cut(s) 2356, 2778
NciI CCSGG 3 cut(s) 534, 3392, 5007
NcoI CCATGG 1 cut(s) 4653
NdeI CATATG 3 cut(s) 116, 2164, 3571
NheI GCTAGC 1 cut(s) 3589
NlaIV GGNNCC 6 cut(s) 648, 745, 1004, 2214, 2571, 3540
NmeAIII GCCGAG 2 cut(s) 1543, 3567
NmuCI GTSAC 7 cut(s) 382, 1344, 2281, 2375, 3727, 4370, 4676
NsiI ATGCAT 3 cut(s) 2138, 2677, 3571
NspI RCATGY 8 cut(s) 92, 172, 623, 731, 1976, 4210, 4963, 5104
OliI CACNNNNGTG 2 cut(s) 2035, 3734
PaeI GCATGC 3 cut(s) 172, 623, 5104
PaeR7I CTCGAG 1 cut(s) 1796
PagI TCATGA 2 cut(s) 3027, 4176
PceI AGGCCT 2 cut(s) 38, 193
PciI ACATGT 2 cut(s) 88, 4959
PcsI WCGNNNNNNNCGW 1 cut(s) 4545
PctI GAATGC 4 cut(s) 1327, 1418, 3338, 4739
PflMI CCANNNNNTGG 2 cut(s) 3499, 5096
PfoI TCCNGGA 2 cut(s) 2625, 3390
PinAI ACCGGT 1 cut(s) 4862
PleI GAGTC 8 cut(s) 109, 200, 430, 1339, 2288, 3000, 3375, 3427
PmaCI CACGTG 1 cut(s) 4343
PmlI CACGTG 1 cut(s) 4343
PpsI GAGTC 8 cut(s) 109, 200, 430, 1339, 2288, 3000, 3375, 3427
Ppu21I YACGTR 1 cut(s) 4343
PpuMI RGGWCCY 1 cut(s) 1792
PscI ACATGT 2 cut(s) 88, 4959
PshAI GACNNNNGTC 1 cut(s) 1790
PshBI ATTAAT 4 cut(s) 63, 1026, 3053, 3174
Psp124BI GAGCTC 2 cut(s) 741, 1921
Psp1406I AACGTT 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 1792
Psp6I CCWGG 8 cut(s) 258, 649, 1005, 1111, 2625, 2652, 2787, 4196
PspCI CACGTG 1 cut(s) 4343
PspEI GGTNACC 2 cut(s) 4370, 4676
PspFI CCCAGC 2 cut(s) 874, 1262
PspGI CCWGG 8 cut(s) 258, 649, 1005, 1111, 2625, 2652, 2787, 4196
PspN4I GGNNCC 6 cut(s) 648, 745, 1004, 2214, 2571, 3540
PspPI GGNCC 8 cut(s) 274, 376, 1003, 1792, 3197, 3667, 3714, 5048
PspPPI RGGWCCY 1 cut(s) 1792
PstI CTGCAG 5 cut(s) 205, 2248, 3013, 3117, 3205
PstNI CAGNNNCTG 5 cut(s) 209, 2626, 3121, 4784, 5124
PvuII CAGCTG 3 cut(s) 3014, 3035, 4427
RseI CAYNNNNRTG 7 cut(s) 299, 833, 2035, 3614, 3734, 3878, 4341
SacI GAGCTC 2 cut(s) 741, 1921
Sau96I GGNCC 8 cut(s) 274, 376, 1003, 1792, 3197, 3667, 3714, 5048
ScaI AGTACT 1 cut(s) 2108
SchI GAGTC 8 cut(s) 110, 200, 431, 1340, 2289, 3001, 3376, 3428
SfcI CTRYAG 8 cut(s) 201, 393, 957, 2244, 3009, 3113, 3201, 3327
Sfr274I CTCGAG 1 cut(s) 1796
SinI GGWCC 4 cut(s) 274, 1792, 3197, 5048
SlaI CTCGAG 1 cut(s) 1796
SmiMI CAYNNNNRTG 7 cut(s) 299, 833, 2035, 3614, 3734, 3878, 4341
SmlI CTYRAG 2 cut(s) 252, 1796
SmoI CTYRAG 2 cut(s) 252, 1796
SphI GCATGC 3 cut(s) 172, 623, 5104
SseBI AGGCCT 2 cut(s) 38, 193
SspI AATATT 2 cut(s) 2765, 4075
SspMI CTAG 9 cut(s) 909, 1994, 2483, 2501, 3156, 3182, 3590, 3968, 4017
SstI GAGCTC 2 cut(s) 741, 1921
StuI AGGCCT 2 cut(s) 38, 193
StyI CCWWGG 3 cut(s) 3754, 4653, 4920
TaiI ACGT 9 cut(s) 83, 1351, 1827, 1957, 2554, 2667, 2812, 3976, 4345
TaqI TCGA 9 cut(s) 1797, 1876, 2274, 2602, 2806, 2955, 3192, 3348, 4539
TatI WGTACW 9 cut(s) 111, 639, 846, 1167, 2106, 2175, 2232, 2415, 3277
TauI GCSGC 4 cut(s) 8, 11, 139, 1987
TseFI GTSAC 7 cut(s) 382, 1344, 2281, 2375, 3727, 4370, 4676
Tsp45I GTSAC 7 cut(s) 382, 1344, 2281, 2375, 3727, 4370, 4676
TspGWI ACGGA 5 cut(s) 2423, 3141, 3332, 4316, 4841
Van91I CCANNNNNTGG 2 cut(s) 3499, 5096
VneI GTGCAC 3 cut(s) 2660, 3975, 4265
VpaK11BI GGWCC 4 cut(s) 274, 1792, 3197, 5048
VspI ATTAAT 4 cut(s) 63, 1026, 3053, 3174
XagI CCTNNNNNAGG 3 cut(s) 2579, 3674, 5064
XapI RAATTY 9 cut(s) 285, 1209, 1238, 1529, 2296, 3263, 3332, 3852, 3905
XbaI TCTAGA 1 cut(s) 3155
XceI RCATGY 8 cut(s) 92, 172, 623, 731, 1976, 4210, 4963, 5104
XcmI CCANNNNNNNNNTGG 2 cut(s) 1658, 3451
XhoI CTCGAG 1 cut(s) 1796
XmiI GTMKAC 2 cut(s) 891, 3549
XspI CTAG 9 cut(s) 909, 1994, 2483, 2501, 3156, 3182, 3590, 3968, 4017
ZrmI AGTACT 1 cut(s) 2108
Zsp2I ATGCAT 3 cut(s) 2138, 2677, 3571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.