AT3G11130

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002740
Physical Location & Seq
Reverse (-)
3482149 .. 3492065
9917 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G11130.1

Sequence Viewer

Length: 5118 bp
ATGGCGGCTGCTAACGCGCCCATCATTATGAAGGAGGTCCTAACGCTTCCGAGCGTTGGGATCGGTCAACAGTTCATCACCTTTACAAATGTTACTATGGAGTCTGATAAGTACATATGTGTACGAGAGACGGCACCGCAGAACAGTGTTGTAATTATCGATATGAATATGCCGATGCAGCCTTTAAGGAGGCCTATAACTGCTGATTCTGCTCTTATGAACCCAAACTCGAGGATCCTTGCCTTAAAAGCTCAAGTTCCAGGAACTACTCAGGATCATCTACAGATATTTAACATTGAAGCAAAGGCAAAGTTGAAGTCACATCAGATGCCTGAGCAGGTTGCTTTTTGGAAGTGGATCACACCTAAGATGCTGGGACTGGTCACACAAACTTCTGTGTATCATTGGTCAATTGAAGGTGATTCTGAGCCAGTTAAGATGTTTGATAGAACGGCTAATTTGGCAAACAATCAAATTATAAACTATAAGTGCTCTCCTAATGAGAAGTGGTTGGTCTTGATTGGAATTGCCCCTGGCTCTCCTGAGAGACCACAATTGGTGAAAGGAAATATGCAGCTTTTCTCTGTGGATCAACAGCGGAGCCAGGCCCTTGAAGCACATGCTGCATCTTTTGCTCAGTTTAAGGTCCCTGGGAATGAGAATCCTTCGATTCTTATATCATTTGCAAGCAAGAGCTTTAATGCTGGGCAGATAACATCAAAGCTGCATGTTATTGAACTTGGTGCCCAACCAGGGAAACCATCATTTACAAAAAAGCAGGCAGATCTCTTTTTCCCCCCAGATTTTGCTGATGATTTTCCTGTGGCCATGCAGGTCTCTCACAAGTTTAACTTGATTTATGTCATCACCAAGCTTGGCCTGCTGTTTGTATATGATCTAGAGACAGCTTCTGCTATCTACAGAAATCGGATAAGTCCGGACCCAATTTTCTTAACGTCTGAAGCTTCCTCAGTTGGGGGTTTCTATGCCATTAATAGGCGAGGACAAGTTCTTTTGGCAACAGTAAATGAGGCCACCATAATACCTTTTATTAGTGGTCAATTGAACAATTTGGAACTTGCTGTCAATCTTGCTAAAAGAGGAAACCTCCCTGGTGCAGAAAATCTGGTTGTCCAGCGGTTCCAAGAGTTATTTGCTCAAACAAAGTACAAGGAGGCTGCTGAGCTTGCTGCTGAATCTCCTCAGGGCATTCTACGGACACCTGATACGGTGGCCAAATTCCAGAGTGTTCCTGTACAAGCAGGGCAAACTCCTCCATTGTTACAGTATTTTGGGACCCTTTTGACTAGAGGGAAGCTCAATTCATATGAGTCTTTGGAACTATCTCGGCTTGTTGTGAATCAAAACAAAAAGAACCTCTTGGAAAACTGGTTGGCAGAGGATAAGTTAGAATGCAGTGAAGAACTCGGAGACCTTGTCAAAACTGTGGATAATGACCTTGCTCTAAAAATATACATCAAAGCTAGAGCTACTCCAAAAGTCGTTGCAGCTTTTGCAGAGCGAAGGGAGTTTGACAAAATATTGATTTATTCAAAGCAGGTTGGGTACACACCTGATTACATGTTTCTTCTGCAAACGATACTCCGTACGGATCCTCAGGGAGCAGTAAATTTTGCTTTAATGATGTCCCAAATGGAAGGAGGTTGTCCAGTTGACTACAACACTATCACTGATCTTTTCCTTCAGAGAAATCTGATCCGTGAAGCGACTGCTTTCCTTCTGGATGTTCTGAAGCCAAATTTACCCGAGCATGCTTTTCTGCAAACTAAGGTTTTGGAGATCAATTTGGTAACCTTTCCTAATGTGGCTGATGCAATCTTAGCAAATGGGATGTTCAGCCATTATGACCGGCCTCGTGTTGCTCAGCTTTGTGAAAAGGCTGGACTTTACATCCAATCTTTAAAGCATTACTCAGAGTTACCTGATATCAAACGTGTAATTGTGAACACACATGCTATTGAGCCACAGGCTCTTGTCGAGTTTTTTGGTACTCTTTCTAGCGAGTGGGCAATGGAGTGCATGAAAGATCTCCTGTTGGTCAACCTGAGAGGCAACCTTCAGATAATCGTTCAGGCTTGCAAGGAGTACTGTGAGCAACTTGGTGTTGATGCATGCATTAAACTCTTTGAGCAATTCAAGTCGTACGAAGGGCTGTACTTTTTCCTAGGTTCATACTTGAGTATGAGTGAGGATCCTGAGATTCACTTCAAGTACATCGAAGCAGCTGCCAAGACTGGTCAAATAAAGGAGGTTGAGCGTGTGACTAGAGAGTCTAACTTTTATGATGCGGAAAAGACGAAGAACTTTTTAATGGAAGCTAAGCTTCCTGATGCCCGACCTTTGATTAATGTCTGTGATCGTTTTGGCTTTGTACCTGATCTTACTCATTACCTCTACACAAACAACATGCTGCGTTACATTGAAGGTTACGTTCAGAAGGTGAATCCTGGGAATGCTCCCTTAGTTGTGGGGCAGTTGCTTGATGACGAATGCCCTGAAGATTTTATAAAAGGCCTCATTCTTTCTGTTCGTTCATTACTTCCTGTTGAACCCCTTGTTGCAGAATGTGAAAAGAGAAATCGACTCCGTCTCCTCACTCAGTTCTTGGAGCATCTAGTTAGTGAGGGAAGCCAAGATGTGCATGTCCATAATGCCTTGGGTAAAATTATCATAGACAGTAACAACAACCCCGAGCATTTCCTGACCACCAATCCTTACTACGACTCTAAGGTTGTGGGTAAGTACTGCGAGAAACGTGATCCCACTCTGGCTGTTGTGGCATACAGAAGAGGACAATGTGACGAGGAACTCATCAATGTCACCAACAAGAACTCTTTGTTCAAGTTACAAGCCAGGTACGTAGTGGAGAGGATGGATGGTGACCTCTGGGAGAAGGTTCTTACGGAAGAAAATGAATATAGGAGGCAACTTATAGACCAAGTTGTGTCTACTGCTTTACCAGAAAGCAAGAGCCCAGAGCAAGTTTCTGCAGCTGTTAAAGCATTCATGACTGCTGATTTGCCACATGAGCTAATTGAGCTTCTGGAAAAAATTGTGCTTCAAAATTCTGCCTTCAGTGGAAATTTCAATCTGCAAAATCTACTTATACTGACAGCTATTAAGGCAGATCCGTCCAGAGTTATGGATTACATTAACAGGCTGGATAATTTCGATGGTCCAGCTGTTGGAGAAGTGGCAGTGGATGCTCAATTGTATGAGGAAGCATTTGCGATTTTCAAGAAGTTTAACTTAAATGTTCAGGCTGTCAACGTATTGTTGGATAACGTCAGAAGCATTGAACGTGCTGTTGAGTTTGCTTTCCGGGTCGAAGAAGATGCTGTTTGGAGTCAAGTCGCTAAGGCACAGCTCAGGGAAGGACTAGTCAGTGATGCTATTGAGTCCTTTATCCGAGCTGATGACACCACTCAGTTCTTGGAGGTCATCCGAGCCTCTGAAGATACTAATGTCTATGATGACTTGGTCAGATACCTTCTGATGGTTAGACAGAAGGTGAAGGAACCCAAGGTGGATAGTGAGCTCATCTATGCTTATGCAAAGATTGAAAGGTTGGGTGAGATTGAAGAATTTATTCTGATGCCCAACGTTGCCAACCTTCAACATGTTGGTGACCGTTTGTACGATGAAGCTCTGTATGAGGCTGCAAAGATAATATATGCATTCATATCGAATTGGGCCAAGTTAGCCGTCACCCTTGTGAAGTTGCAACAGTTCCAAGGTGCTGTTGATGCTGCAAGGAAAGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGTGTTGATGCTGAGGAATTCCGACTGGCTCAAATCTGTGGACTTAACATTATCATACAGGTGGATGACCTGGAAGAAGTAAGCGAGTACTACCAGAACAGAGGATGCTTCAATGAATTGATCTCCCTTATGGAGAGTGGACTTGGTCTGGAACGTGCTCACATGGGTATCTTCACCGAGTTAGGAGTACTGTACGCCAGATATCGTTATGAGAAGCTTATGGAACACATCAAGTTGTTCTCTACTCGACTCAATATTCCCAAGCTTATCCGGGCCTGTGATGAACAACAGCATTGGCAGGAACTTACCTATCTTTACATTCAATATGATGAGTTTGATAATGCTGCAACCACTGTTATGAATCATTCTCCTGAAGCATGGGAGCACATGCAATTCAAAGACATTGTTGCCAAGGTTGCGAATGTTGAGCTTTACTATAAGGCCGTTCACTTCTACTTGCAAGAGCACCCTGATATAATCAACGATCTTCTCAATGTGCTTGCTCTGCGGTTAGATCACACGCGTGTCGTTGATATTATGCGCAAGGCTGGTCACTTGCGCCTTATTAAGCCATATATGGTTGCAGTTCAGAGCAATAATGTATCTGCTGTAAATGAAGCTCTGAATGAGATATATGCAGAAGAAGAAGACTATGACAGGTTGCGCGAGTCTATTGATTTGCATGACAGTTTTGACCAGATAGGTCTCGCTCAGAAGATTGAGAAACACGAGCTTGTTGAAATGAGACGTGTGGCTGCTTATATCTATAAGAAAGCTGGTAGATGGAAGCAATCGATTGCTTTGTCAAAGAAAGATAACATGTACAAGGACTGTATGGAAACTGCTTCGCAATCCGGCGACCATGATCTTGCAGAACAACTTCTCGTCTACTTCATTGAACAGGGGAAAAAGGAATGCTTTGCTACATGTCTCTTTGTCTGTTATGACTTAATCCGACCAGACGTTGCTCTAGAACTTGCTTGGATCAACAATATGATCGACTTTGCCTTCCCGTATCTCCTCCAGTTTATCCGAGAATACTCCGGCAAAGTGGATGAGCTAATCAAGGACAAGCTGGAGGCACAAAAAGAGGTGAAGGCCAAAGAACAGGAAGAGAAGGATGTCATGTCACAACAGAACATGTACGCCCAATTATTACCACTGGCTCTACCCGCACCACCGATGCCAGGAATGGGAGGTGGCGGGTATGGTCCACCACCACAAATGGGTGGAATGCCAGGAATGTCAGGAATGCCTCCAATGCCTCCATATGGAATGCCACCGATGGGCGGCTACTAA

Protein Analysis

1705

Amino Acids

193.24

Weight (kDa)

5.26

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_propel PF01394 20 - 56 1.1e-07 Clathrin propeller repeat
Clathrin-link PF09268 344 - 367 3.4e-09 Clathrin, heavy-chain linker
Clathrin_H_link PF13838 369 - 434 4e-28 Clathrin-H-link
Clathrin PF00637 557 - 692 7.3e-25 Region in Clathrin and VPS
Clathrin PF00637 701 - 841 6.1e-27 Region in Clathrin and VPS
Clathrin PF00637 850 - 984 5.1e-33 Region in Clathrin and VPS
Clathrin PF00637 993 - 1131 2.3e-31 Region in Clathrin and VPS
Clathrin PF00637 1144 - 1281 2.3e-31 Region in Clathrin and VPS
Clathrin PF00637 1290 - 1431 2e-26 Region in Clathrin and VPS
Clathrin PF00637 1438 - 1579 3.3e-33 Region in Clathrin and VPS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 479, 2528
AasI GACNNNNNNGTC 1 cut(s) 2290
Acc16I TGCGCA 1 cut(s) 4361
Acc36I ACCTGC 3 cut(s) 328, 823, 1549
AccB1I GGYRCC 2 cut(s) 133, 743
AccB7I CCANNNNNTGG 1 cut(s) 3206
AccI GTMKAC 2 cut(s) 2969, 4707
AccII CGCG 3 cut(s) 17, 4342, 4485
AccIII TCCGGA 1 cut(s) 937
AciI CCGC 9 cut(s) 5, 137, 598, 1138, 2309, 4327, 4992, 5022, 5109
AclI AACGTT 1 cut(s) 3624
AcoI YGGCCR 2 cut(s) 825, 1233
AcsI RAATTY 7 cut(s) 1238, 1630, 1759, 3085, 3103, 3605, 3836
AcuI CTGAAG 8 cut(s) 981, 1688, 1772, 2063, 2538, 3079, 3495, 4212
AflIII ACRYGT 8 cut(s) 1581, 1954, 3640, 4340, 4567, 4638, 4745, 4959
AhlI ACTAGT 1 cut(s) 3400
AjiI CACGTC 1 cut(s) 4568
AleI CACNNNNGTG 2 cut(s) 3734, 4341
Alw21I GWGCWC 5 cut(s) 494, 3561, 3979, 4206, 4287
Alw26I GTCTC 9 cut(s) 122, 541, 841, 896, 1425, 2615, 4529, 4558, 4754
AlwNI CAGNNNCTG 1 cut(s) 911
Ama87I CYCGRG 3 cut(s) 229, 1766, 2711
Aor13HI TCCGGA 1 cut(s) 937
ApoI RAATTY 7 cut(s) 1238, 1630, 1759, 3085, 3103, 3605, 3836
ArsI GACNNNNNNTTYG 4 cut(s) 302, 334, 3022, 3054
AseI ATTAAT 2 cut(s) 993, 2367
Asp700I GAANNNNTTC 2 cut(s) 3609, 4698
AspA2I CCTAGG 1 cut(s) 2185
AspLEI GCGC 4 cut(s) 19, 4362, 4380, 4485
AspS9I GGNCC 9 cut(s) 37, 607, 646, 940, 1296, 3197, 3714, 4092, 5030
AsuC2I CCSGG 2 cut(s) 3344, 4091
AvaI CYCGRG 3 cut(s) 229, 1766, 2711
AvaII GGWCC 6 cut(s) 37, 646, 940, 1296, 3197, 5030
AvrII CCTAGG 1 cut(s) 2185
AxyI CCTNAGG 2 cut(s) 1203, 1617
BaeGI GKGCMC 1 cut(s) 748
BaeI ACNNNNGTAYC 4 cut(s) 3471, 3504, 3970, 4003
BalI TGGCCA 2 cut(s) 827, 1235
BamHI GGATCC 3 cut(s) 234, 1612, 2212
BanI GGYRCC 2 cut(s) 133, 743
BanII GRGCYC 2 cut(s) 2996, 3561
BauI CACGAG 2 cut(s) 1875, 4547
BbsI GAAGAC 1 cut(s) 4473
Bbv12I GWGCWC 5 cut(s) 494, 3561, 3979, 4206, 4287
BbvCI CCTCAGC 1 cut(s) 3831
BccI CCATC 8 cut(s) 29, 769, 2887, 2891, 3188, 3511, 4596, 5098
BceAI ACGGC 4 cut(s) 147, 468, 3710, 4247
BcgI CGANNNNNNTGC 6 cut(s) 2228, 2262, 3338, 3372, 3687, 3721
BcnI CCSGG 2 cut(s) 3344, 4091
BcoDI GTCTC 9 cut(s) 122, 541, 841, 896, 1425, 2615, 4529, 4558, 4754
BcuI ACTAGT 1 cut(s) 3400
BfaI CTAG 9 cut(s) 899, 1308, 1485, 2019, 2186, 2286, 2636, 3401, 4790
BfmI CTRYAG 3 cut(s) 281, 919, 3009
BfuAI ACCTGC 3 cut(s) 328, 823, 1549
BglII AGATCT 2 cut(s) 784, 2047
BlnI CCTAGG 1 cut(s) 2185
BlpI GCTNAGC 3 cut(s) 1182, 1884, 2340
BmcAI AGTACT 4 cut(s) 2108, 2765, 3908, 4008
Bme18I GGWCC 6 cut(s) 37, 646, 940, 1296, 3197, 5030
BmeT110I CYCGRG 3 cut(s) 229, 1766, 2711
BmgBI CACGTC 1 cut(s) 4568
BmgT120I GGNCC 9 cut(s) 37, 607, 646, 940, 1296, 3197, 3714, 4092, 5030
BpiI GAAGAC 1 cut(s) 4473
BplI GAGNNNNNCTC 4 cut(s) 1092, 1124, 1302, 1334
BpmI CTGGAG 2 cut(s) 4826, 4916
Bpu10I CCTNAGC 4 cut(s) 333, 3378, 3389, 3831
Bpu1102I GCTNAGC 3 cut(s) 1182, 1884, 2340
BpuEI CTTGAG 2 cut(s) 237, 2218
BpuMI CCSGG 2 cut(s) 3344, 4091
Bsa29I ATCGAT 2 cut(s) 159, 4613
BsaAI YACGTR 1 cut(s) 2881
BsaI GGTCTC 4 cut(s) 541, 841, 1425, 4529
BsaWI WCCGGW 1 cut(s) 937
BsaXI ACNNNNNCTCC 4 cut(s) 1422, 1452, 2595, 2625
Bse118I RCCGGY 1 cut(s) 1869
Bse1I ACTGG 8 cut(s) 384, 431, 1394, 1670, 2260, 3849, 4843, 4986
Bse21I CCTNAGG 2 cut(s) 1203, 1617
Bse3DI GCAATG 1 cut(s) 2037
BseAI TCCGGA 1 cut(s) 937
BseCI ATCGAT 2 cut(s) 159, 4613
BseMI GCAATG 1 cut(s) 2037
BseNI ACTGG 8 cut(s) 384, 431, 1394, 1670, 2260, 3849, 4843, 4986
BseRI GAGGAG 4 cut(s) 1191, 1263, 2603, 4829
BseSI GKGCMC 1 cut(s) 748
BseYI CCCAGC 2 cut(s) 373, 704
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 3 cut(s) 17, 4342, 4485
BshNI GGYRCC 2 cut(s) 133, 743
BshVI ATCGAT 2 cut(s) 159, 4613
BsiHKAI GWGCWC 5 cut(s) 494, 3561, 3979, 4206, 4287
BsiHKCI CYCGRG 3 cut(s) 229, 1766, 2711
BsiSI CCGG 6 cut(s) 938, 1870, 3343, 4090, 4674, 4863
BsiWI CGTACG 2 cut(s) 1607, 2163
BslFI GGGAC 4 cut(s) 390, 632, 1309, 1633
BsmAI GTCTC 9 cut(s) 122, 541, 841, 896, 1425, 2615, 4529, 4558, 4754
BsmBI CGTCTC 3 cut(s) 122, 2615, 4558
BsmFI GGGAC 4 cut(s) 390, 632, 1309, 1633
Bso31I GGTCTC 4 cut(s) 541, 841, 1425, 4529
BsoBI CYCGRG 3 cut(s) 229, 1766, 2711
Bsp1286I GDGCHC 7 cut(s) 494, 748, 2996, 3561, 3979, 4206, 4287
Bsp13I TCCGGA 1 cut(s) 937
Bsp1407I TGTACA 2 cut(s) 1255, 4641
Bsp1720I GCTNAGC 3 cut(s) 1182, 1884, 2340
BspACI CCGC 9 cut(s) 5, 137, 598, 1138, 2309, 4327, 4992, 5022, 5109
BspDI ATCGAT 2 cut(s) 159, 4613
BspEI TCCGGA 1 cut(s) 937
BspFNI CGCG 3 cut(s) 17, 4342, 4485
BspHI TCATGA 1 cut(s) 3027
BspMAI CTGCAG 1 cut(s) 3013
BspMI ACCTGC 3 cut(s) 328, 823, 1549
BspT107I GGYRCC 2 cut(s) 133, 743
BspTNI GGTCTC 4 cut(s) 541, 841, 1425, 4529
BsrDI GCAATG 1 cut(s) 2037
BsrFI RCCGGY 1 cut(s) 1869
BsrGI TGTACA 2 cut(s) 1255, 4641
BsrI ACTGG 8 cut(s) 384, 431, 1394, 1670, 2260, 3849, 4843, 4986
BssAI RCCGGY 1 cut(s) 1869
BssSI CACGAG 2 cut(s) 1875, 4547
BssT1I CCWWGG 5 cut(s) 2185, 2676, 3543, 3754, 4230
Bst2BI CACGAG 2 cut(s) 1875, 4547
Bst6I CTCTTC 2 cut(s) 2803, 4926
BstAPI GCANNNNNTGC 5 cut(s) 623, 632, 1514, 3224, 3788
BstAUI TGTACA 2 cut(s) 1255, 4641
BstBAI YACGTR 1 cut(s) 2881
BstC8I GCNNGC 8 cut(s) 688, 780, 881, 1188, 1773, 2098, 2134, 4320
BstEII GGTNACC 3 cut(s) 1810, 2900, 3647
BstFNI CGCG 3 cut(s) 17, 4342, 4485
BstHHI GCGC 4 cut(s) 19, 4362, 4380, 4485
BstMAI GTCTC 9 cut(s) 122, 541, 841, 896, 1425, 2615, 4529, 4558, 4754
BstPI GGTNACC 3 cut(s) 1810, 2900, 3647
BstSFI CTRYAG 3 cut(s) 281, 919, 3009
BstSLI GKGCMC 1 cut(s) 748
BstSNI TACGTA 1 cut(s) 2881
BstUI CGCG 3 cut(s) 17, 4342, 4485
BstV2I GAAGAC 1 cut(s) 4473
BstX2I RGATCY 6 cut(s) 234, 784, 1612, 2047, 2212, 3148
BstXI CCANNNNNNTGG 1 cut(s) 3163
BstYI RGATCY 6 cut(s) 234, 784, 1612, 2047, 2212, 3148
Bsu15I ATCGAT 2 cut(s) 159, 4613
Bsu36I CCTNAGG 2 cut(s) 1203, 1617
BsuTUI ATCGAT 2 cut(s) 159, 4613
BtrI CACGTC 1 cut(s) 4568
BtsI GCAGTG 2 cut(s) 1423, 3225
BtsIMutI CAGTG 9 cut(s) 151, 1423, 1689, 3103, 3225, 3412, 3793, 4170, 4979
BveI ACCTGC 3 cut(s) 328, 823, 1549
Cac8I GCNNGC 8 cut(s) 688, 780, 881, 1188, 1773, 2098, 2134, 4320
CaiI CAGNNNCTG 1 cut(s) 911
CciI TCATGA 1 cut(s) 3027
CfoI GCGC 4 cut(s) 19, 4362, 4380, 4485
Cfr10I RCCGGY 1 cut(s) 1869
Cfr13I GGNCC 9 cut(s) 37, 607, 646, 940, 1296, 3197, 3714, 4092, 5030
ClaI ATCGAT 2 cut(s) 159, 4613
DraI TTTAAA 1 cut(s) 1923
DrdI GACNNNNNNGTC 1 cut(s) 2290
DseDI GACNNNNNNGTC 1 cut(s) 2290
EaeI YGGCCR 2 cut(s) 825, 1233
Eam1104I CTCTTC 2 cut(s) 2803, 4926
EarI CTCTTC 2 cut(s) 2803, 4926
Ecl136II GAGCTC 1 cut(s) 3559
Eco105I TACGTA 1 cut(s) 2881
Eco130I CCWWGG 5 cut(s) 2185, 2676, 3543, 3754, 4230
Eco147I AGGCCT 2 cut(s) 193, 2535
Eco24I GRGCYC 2 cut(s) 2996, 3561
Eco31I GGTCTC 4 cut(s) 541, 841, 1425, 4529
Eco32I GATATC 2 cut(s) 1948, 4022
Eco47I GGWCC 6 cut(s) 37, 646, 940, 1296, 3197, 5030
Eco53kI GAGCTC 1 cut(s) 3559
Eco57I CTGAAG 8 cut(s) 981, 1688, 1772, 2063, 2538, 3079, 3495, 4212
Eco81I CCTNAGG 2 cut(s) 1203, 1617
Eco88I CYCGRG 3 cut(s) 229, 1766, 2711
Eco91I GGTNACC 3 cut(s) 1810, 2900, 3647
EcoICRI GAGCTC 1 cut(s) 3559
EcoO109I RGGNCCY 4 cut(s) 37, 607, 646, 1296
EcoO65I GGTNACC 3 cut(s) 1810, 2900, 3647
EcoRI GAATTC 1 cut(s) 3836
EcoRV GATATC 2 cut(s) 1948, 4022
EcoT14I CCWWGG 5 cut(s) 2185, 2676, 3543, 3754, 4230
EcoT22I ATGCAT 3 cut(s) 2134, 2138, 3700
EcoT38I GRGCYC 2 cut(s) 2996, 3561
ErhI CCWWGG 5 cut(s) 2185, 2676, 3543, 3754, 4230
Esp3I CGTCTC 3 cut(s) 122, 2615, 4558
FaqI GGGAC 4 cut(s) 390, 632, 1309, 1633
FauI CCCGC 2 cut(s) 4999, 5015
FauNDI CATATG 3 cut(s) 116, 1327, 5089
FblI GTMKAC 2 cut(s) 2969, 4707
FriOI GRGCYC 2 cut(s) 2996, 3561
FspBI CTAG 9 cut(s) 899, 1308, 1485, 2019, 2186, 2286, 2636, 3401, 4790
FspI TGCGCA 1 cut(s) 4361
GlaI GCGC 4 cut(s) 18, 4361, 4379, 4484
GsaI CCCAGC 2 cut(s) 377, 708
GsuI CTGGAG 2 cut(s) 4826, 4916
HapII CCGG 6 cut(s) 938, 1870, 3343, 4090, 4674, 4863
HhaI GCGC 4 cut(s) 19, 4362, 4380, 4485
Hin6I GCGC 4 cut(s) 17, 4360, 4378, 4483
HinP1I GCGC 4 cut(s) 17, 4360, 4378, 4483
HincII GTYRAC 4 cut(s) 68, 1675, 2062, 3289
HindII GTYRAC 4 cut(s) 68, 1675, 2062, 3289
HindIII AAGCTT 5 cut(s) 872, 963, 2342, 4034, 4082
HpaII CCGG 6 cut(s) 938, 1870, 3343, 4090, 4674, 4863
HspAI GCGC 4 cut(s) 17, 4360, 4378, 4483
KflI GGGWCCC 1 cut(s) 1296
Kpn2I TCCGGA 1 cut(s) 937
LmnI GCTCC 5 cut(s) 600, 1622, 2482, 2629, 4201
MaeI CTAG 9 cut(s) 899, 1308, 1485, 2019, 2186, 2286, 2636, 3401, 4790
MfeI CAATTG 4 cut(s) 411, 554, 1061, 3230
MflI RGATCY 6 cut(s) 234, 784, 1612, 2047, 2212, 3148
MhlI GDGCHC 7 cut(s) 494, 748, 2996, 3561, 3979, 4206, 4287
MlsI TGGCCA 2 cut(s) 827, 1235
MluI ACGCGT 1 cut(s) 4340
MluNI TGGCCA 2 cut(s) 827, 1235
MlyI GAGTC 9 cut(s) 110, 1340, 2300, 2598, 2738, 3376, 3428, 4062, 4496
MmeI TCCRAC 4 cut(s) 3187, 3279, 3865, 4798
Mox20I TGGCCA 2 cut(s) 827, 1235
Mph1103I ATGCAT 3 cut(s) 2134, 2138, 3700
MroI TCCGGA 1 cut(s) 937
MroXI GAANNNNTTC 2 cut(s) 3609, 4698
MscI TGGCCA 2 cut(s) 827, 1235
MslI CAYNNNNRTG 6 cut(s) 26, 3645, 3734, 3878, 4175, 4341
Msp20I TGGCCA 2 cut(s) 827, 1235
MspA1I CMGCKG 5 cut(s) 598, 1138, 2246, 3014, 3203
MspI CCGG 6 cut(s) 938, 1870, 3343, 4090, 4674, 4863
MunI CAATTG 4 cut(s) 411, 554, 1061, 3230
MvnI CGCG 3 cut(s) 17, 4342, 4485
NciI CCSGG 2 cut(s) 3344, 4091
NdeI CATATG 3 cut(s) 116, 1327, 5089
NmeAIII GCCGAG 1 cut(s) 1327
NsbI TGCGCA 1 cut(s) 4361
NsiI ATGCAT 3 cut(s) 2134, 2138, 3700
OliI CACNNNNGTG 2 cut(s) 3734, 4341
PaeI GCATGC 2 cut(s) 1775, 2136
PaeR7I CTCGAG 1 cut(s) 229
PagI TCATGA 1 cut(s) 3027
PasI CCCWGGG 1 cut(s) 650
PceI AGGCCT 2 cut(s) 193, 2535
PciI ACATGT 5 cut(s) 1581, 3640, 4638, 4745, 4959
PdmI GAANNNNTTC 2 cut(s) 3609, 4698
PfeI GAWTC 9 cut(s) 206, 422, 661, 670, 1196, 1360, 2221, 2464, 4180
Pfl23II CGTACG 2 cut(s) 1607, 2163
PflFI GACNNNGTC 4 cut(s) 1436, 2607, 3500, 3963
PflMI CCANNNNNTGG 1 cut(s) 3206
PfoI TCCNGGA 1 cut(s) 259
PleI GAGTC 9 cut(s) 109, 1339, 2299, 2598, 2738, 3375, 3427, 4062, 4495
PpsI GAGTC 9 cut(s) 109, 1339, 2299, 2598, 2738, 3375, 3427, 4062, 4495
Ppu21I YACGTR 1 cut(s) 2881
PpuMI RGGWCCY 3 cut(s) 37, 646, 1296
PscI ACATGT 5 cut(s) 1581, 3640, 4638, 4745, 4959
PshBI ATTAAT 2 cut(s) 993, 2367
PsiI TTATAA 2 cut(s) 479, 2528
Psp124BI GAGCTC 1 cut(s) 3561
Psp1406I AACGTT 1 cut(s) 3624
Psp5II RGGWCCY 3 cut(s) 37, 646, 1296
PspEI GGTNACC 3 cut(s) 1810, 2900, 3647
PspFI CCCAGC 2 cut(s) 373, 704
PspLI CGTACG 2 cut(s) 1607, 2163
PspPI GGNCC 9 cut(s) 37, 607, 646, 940, 1296, 3197, 3714, 4092, 5030
PspPPI RGGWCCY 3 cut(s) 37, 646, 1296
PspXI VCTCGAGB 1 cut(s) 229
PstI CTGCAG 1 cut(s) 3013
PstNI CAGNNNCTG 1 cut(s) 911
PsuI RGATCY 6 cut(s) 234, 784, 1612, 2047, 2212, 3148
PsyI GACNNNGTC 4 cut(s) 1436, 2607, 3500, 3963
PvuII CAGCTG 3 cut(s) 2246, 3014, 3203
RseI CAYNNNNRTG 6 cut(s) 26, 3645, 3734, 3878, 4175, 4341
SacI GAGCTC 1 cut(s) 3561
Sau96I GGNCC 9 cut(s) 37, 607, 646, 940, 1296, 3197, 3714, 4092, 5030
ScaI AGTACT 4 cut(s) 2108, 2765, 3908, 4008
SchI GAGTC 9 cut(s) 110, 1340, 2300, 2598, 2738, 3376, 3428, 4062, 4496
SduI GDGCHC 7 cut(s) 494, 748, 2996, 3561, 3979, 4206, 4287
SfcI CTRYAG 3 cut(s) 281, 919, 3009
Sfr274I CTCGAG 1 cut(s) 229
SinI GGWCC 6 cut(s) 37, 646, 940, 1296, 3197, 5030
SlaI CTCGAG 1 cut(s) 229
SmiMI CAYNNNNRTG 6 cut(s) 26, 3645, 3734, 3878, 4175, 4341
SmlI CTYRAG 3 cut(s) 229, 252, 2197
SmoI CTYRAG 3 cut(s) 229, 252, 2197
SnaBI TACGTA 1 cut(s) 2881
SpeI ACTAGT 1 cut(s) 3400
SphI GCATGC 2 cut(s) 1775, 2136
SseBI AGGCCT 2 cut(s) 193, 2535
SsiI CCGC 9 cut(s) 5, 137, 598, 1138, 2309, 4327, 4992, 5022, 5109
SspI AATATT 2 cut(s) 1542, 4075
SspMI CTAG 9 cut(s) 899, 1308, 1485, 2019, 2186, 2286, 2636, 3401, 4790
SstI GAGCTC 1 cut(s) 3561
StuI AGGCCT 2 cut(s) 193, 2535
StyI CCWWGG 5 cut(s) 2185, 2676, 3543, 3754, 4230
TaqII GACCGA 1 cut(s) 53
TauI GCSGC 2 cut(s) 8, 5112
TfiI GAWTC 9 cut(s) 206, 422, 661, 670, 1196, 1360, 2221, 2464, 4180
TscAI CASTG 9 cut(s) 151, 1423, 1696, 3103, 3225, 3412, 3793, 4177, 4986
TspGWI ACGGA 7 cut(s) 1231, 1595, 1625, 1709, 2597, 2939, 3141
TspRI CASTG 9 cut(s) 151, 1423, 1696, 3103, 3225, 3412, 3793, 4177, 4986
Tth111I GACNNNGTC 4 cut(s) 1436, 2607, 3500, 3963
Van91I CCANNNNNTGG 1 cut(s) 3206
VpaK11BI GGWCC 6 cut(s) 37, 646, 940, 1296, 3197, 5030
VspI ATTAAT 2 cut(s) 993, 2367
XapI RAATTY 7 cut(s) 1238, 1630, 1759, 3085, 3103, 3605, 3836
XbaI TCTAGA 2 cut(s) 898, 4789
XcmI CCANNNNNNNNNTGG 2 cut(s) 2881, 3451
XhoI CTCGAG 1 cut(s) 229
XmaJI CCTAGG 1 cut(s) 2185
XmiI GTMKAC 2 cut(s) 2969, 4707
XmnI GAANNNNTTC 2 cut(s) 3609, 4698
XspI CTAG 9 cut(s) 899, 1308, 1485, 2019, 2186, 2286, 2636, 3401, 4790
ZrmI AGTACT 4 cut(s) 2108, 2765, 3908, 4008
Zsp2I ATGCAT 3 cut(s) 2134, 2138, 3700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.