AT3G16700

Acylpyruvase FAHD1, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002365
Physical Location & Seq
Forward (+)
5687696 .. 5689796
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16700.4

Sequence Viewer

Length: 675 bp
ATGGCGACTTCGATGATTCAGAGATTGTTCAAACAAGGCACAAAGATCGTCGGCGTTGGACTCAATTACGCTTCTCACGCCAAAGAACTTGGCAATGCCCTCCCAAAGGACCCGATTGTGTTCTTAAAGCCAACTTCGTCGTACCTGGAAAACGGTGGAACAATAGAGATCCCACATCCACTGGATTCACTTCACCATGAGGTGGAACTTGCTGTAGTAATTGGACAGAAAGCAAGAGATGTCCCAGAACGGTTAGCCATGAATTACATTGGAGGATATGCGCTTGCTCTTGATATGACTGCTAGGGAACTCCAAGTTTCTGCCATGGCATCTGGTCTCCCATGTACGCTTGCTAAAGGACAGGATACCTTCACCCCCATCAGCTCCGTTCTACCAAAGGCAATGGTGCTGGATCCCAATAACCTGGAACTCTGGCTAAAGGTAGATGATGAGACAAGACAGAAAGGTTGGACAAAGGATATGATCTTCAAGGTCCCATATCTCATCAGCTACATAAGTTCTGTAATGACCCTTTTCAAAGGAGATGTCATATTGACAGGTACACCAGAAGGTATAGGACCTGTAAAGATAGGTCAGAAGATAACCGCCGGAATCACCGGTCTTTCTGAAGTTCAGTTCGACGTCGGTAGGCGGCTAAAGCCATTGTTGAGATAG

Protein Analysis

224

Amino Acids

24.42

Weight (kDa)

8.56

Isoelectric Point (pI)

24.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 15 - 109 6.9e-27 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 645
AccB7I CCANNNNNTGG 1 cut(s) 202
AciI CCGC 2 cut(s) 606, 652
AclWI GGATC 3 cut(s) 163, 407, 420
AcuI CTGAAG 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 642
AfaI GTAC 3 cut(s) 143, 346, 562
AfiI CCNNNNNNNGG 2 cut(s) 106, 202
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 3 cut(s) 31, 490, 538
AjnI CCWGG 2 cut(s) 144, 423
AluBI AGCT 2 cut(s) 384, 510
AluI AGCT 2 cut(s) 384, 510
Alw26I GTCTC 2 cut(s) 341, 446
AlwI GGATC 3 cut(s) 163, 407, 420
ArsI GACNNNNNNTTYG 2 cut(s) 531, 563
AsiGI ACCGGT 1 cut(s) 617
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 3 cut(s) 109, 493, 578
AsuHPI GGTGA 3 cut(s) 185, 364, 607
AvaII GGWCC 3 cut(s) 109, 493, 578
BamHI GGATCC 1 cut(s) 412
BccI CCATC 1 cut(s) 386
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 2 cut(s) 146, 425
BciVI GTATCC 1 cut(s) 358
BcoDI GTCTC 2 cut(s) 341, 446
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 213
BfuI GTATCC 1 cut(s) 358
BisI GCNGC 1 cut(s) 653
BlsI GCNGC 1 cut(s) 654
Bme1390I CCNGG 2 cut(s) 146, 425
Bme18I GGWCC 3 cut(s) 109, 493, 578
BmgT120I GGNCC 3 cut(s) 109, 493, 578
BmiI GGNNCC 3 cut(s) 111, 414, 495
BmrFI CCNGG 2 cut(s) 146, 425
BmsI GCATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 617
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 202
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 1 cut(s) 186
Bse3DI GCAATG 2 cut(s) 100, 408
BseBI CCWGG 2 cut(s) 146, 425
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 106, 202
BseMI GCAATG 2 cut(s) 100, 408
BseNI ACTGG 1 cut(s) 186
BshTI ACCGGT 1 cut(s) 617
BsiSI CCGG 2 cut(s) 609, 618
BslFI GGGAC 2 cut(s) 227, 479
BslI CCNNNNNNNGG 2 cut(s) 106, 202
BsmAI GTCTC 2 cut(s) 341, 446
BsmFI GGGAC 2 cut(s) 227, 479
Bso31I GGTCTC 1 cut(s) 341
Bsp143I GATC 4 cut(s) 45, 168, 412, 483
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 2 cut(s) 606, 652
BspLI GGNNCC 3 cut(s) 111, 414, 495
BspPI GGATC 3 cut(s) 163, 407, 420
BspTNI GGTCTC 1 cut(s) 341
BsrDI GCAATG 2 cut(s) 100, 408
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 4 cut(s) 45, 168, 412, 483
BssNI GRCGYC 1 cut(s) 642
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 146, 425
Bst4CI ACNGT 2 cut(s) 155, 252
BstACI GRCGYC 1 cut(s) 642
BstC8I GCNNGC 2 cut(s) 285, 351
BstDSI CCRYGG 1 cut(s) 324
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 1 cut(s) 175
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 4 cut(s) 48, 171, 415, 486
BstMAI GTCTC 2 cut(s) 341, 446
BstMBI GATC 4 cut(s) 45, 168, 412, 483
BstMWI GCNNNNNNNGC 2 cut(s) 77, 658
BstNI CCWGG 2 cut(s) 146, 425
BstSCI CCNGG 2 cut(s) 144, 423
BstSFI CTRYAG 1 cut(s) 213
BstX2I RGATCY 2 cut(s) 168, 412
BstXI CCANNNNNNTGG 1 cut(s) 424
BstYI RGATCY 2 cut(s) 168, 412
BsuI GTATCC 1 cut(s) 358
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 285, 351
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 3 cut(s) 109, 493, 578
Csp6I GTAC 3 cut(s) 142, 345, 561
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 4 cut(s) 197, 259, 325, 342
CviJI RGCY 7 cut(s) 130, 257, 384, 436, 510, 655, 661
CviKI_1 RGCY 7 cut(s) 130, 257, 384, 436, 510, 655, 661
CviQI GTAC 3 cut(s) 142, 345, 561
DpnI GATC 4 cut(s) 47, 170, 414, 485
DpnII GATC 4 cut(s) 45, 168, 412, 483
Eco130I CCWWGG 1 cut(s) 324
Eco31I GGTCTC 1 cut(s) 341
Eco47I GGWCC 3 cut(s) 109, 493, 578
Eco57I CTGAAG 1 cut(s) 648
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoO109I RGGNCCY 3 cut(s) 109, 493, 578
EcoRII CCWGG 2 cut(s) 144, 423
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 4 cut(s) 200, 262, 328, 345
FaqI GGGAC 2 cut(s) 227, 479
FatI CATG 4 cut(s) 196, 258, 324, 341
Fnu4HI GCNGC 1 cut(s) 653
FokI GGATG 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 653
FspBI CTAG 1 cut(s) 303
GlaI GCGC 1 cut(s) 282
GluI GCNGC 1 cut(s) 653
HapII CCGG 2 cut(s) 609, 618
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 642
Hin1II CATG 4 cut(s) 200, 262, 328, 345
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 4 cut(s) 16, 60, 185, 612
HpaII CCGG 2 cut(s) 609, 618
HphI GGTGA 3 cut(s) 185, 364, 607
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 3 cut(s) 21, 597, 628
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 1 cut(s) 563
Hpy99I CGWCG 4 cut(s) 53, 142, 644, 647
HpyAV CCTTC 2 cut(s) 379, 563
HpyCH4III ACNGT 2 cut(s) 155, 252
HpyCH4IV ACGT 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 658
HpySE526I ACGT 1 cut(s) 642
Hsp92I GRCGYC 1 cut(s) 642
Hsp92II CATG 4 cut(s) 200, 262, 328, 345
HspAI GCGC 1 cut(s) 281
Kzo9I GATC 4 cut(s) 45, 168, 412, 483
LmnI GCTCC 1 cut(s) 389
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 642
MalI GATC 4 cut(s) 47, 170, 414, 485
MboI GATC 4 cut(s) 45, 168, 412, 483
MboII GAAGA 2 cut(s) 478, 610
MflI RGATCY 2 cut(s) 168, 412
MluCI AATT 3 cut(s) 64, 219, 262
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 2 cut(s) 37, 449
MnlI CCTC 3 cut(s) 110, 193, 266
MseI TTAA 1 cut(s) 125
MspI CCGG 2 cut(s) 609, 618
MspR9I CCNGG 2 cut(s) 146, 425
MvaI CCWGG 2 cut(s) 146, 425
MwoI GCNNNNNNNGC 2 cut(s) 77, 658
NcoI CCATGG 1 cut(s) 324
NdeII GATC 4 cut(s) 45, 168, 412, 483
NlaIII CATG 4 cut(s) 200, 262, 328, 345
NlaIV GGNNCC 3 cut(s) 111, 414, 495
PfeI GAWTC 3 cut(s) 16, 185, 612
PflMI CCANNNNNTGG 1 cut(s) 202
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 1 cut(s) 654
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 3 cut(s) 109, 493, 578
Psp5II RGGWCCY 3 cut(s) 109, 493, 578
Psp6I CCWGG 2 cut(s) 144, 423
PspGI CCWGG 2 cut(s) 144, 423
PspN4I GGNNCC 3 cut(s) 111, 414, 495
PspPI GGNCC 3 cut(s) 109, 493, 578
PspPPI RGGWCCY 3 cut(s) 109, 493, 578
PsuI RGATCY 2 cut(s) 168, 412
RsaI GTAC 3 cut(s) 143, 346, 562
RsaNI GTAC 3 cut(s) 142, 345, 561
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 1 cut(s) 653
Sau3AI GATC 4 cut(s) 45, 168, 412, 483
Sau96I GGNCC 3 cut(s) 109, 493, 578
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 146, 425
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 3 cut(s) 109, 493, 578
Sse9I AATT 3 cut(s) 64, 219, 262
SsiI CCGC 2 cut(s) 606, 652
SspMI CTAG 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 144, 423
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 155, 252
TaiI ACGT 1 cut(s) 645
TaqI TCGA 2 cut(s) 11, 639
TasI AATT 3 cut(s) 64, 219, 262
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 3 cut(s) 16, 185, 612
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 186
TspDTI ATGAA 1 cut(s) 275
TspGWI ACGGA 1 cut(s) 376
TspRI CASTG 1 cut(s) 186
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 3 cut(s) 109, 493, 578
XagI CCTNNNNNAGG 1 cut(s) 104
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.