Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002365

Physical Location & Seq

Genomic Coordinates Reverse (-)

2425437 .. 2433340

Gene Structure 7904 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1020300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 426 bp

ATGAACGGCGGCACGATCGAAGTCCCTCACCCTCTGTTATCTTTGGACCATGAGGTGGCGGTGATCATCTCTCAGAAAGCTCACGATGTTCCGCAATCCACCGCCATGGATTACATTGCGGGTTATGCACTTGCTTTGGATATAACTGCCAGAGAAATTCAAGCATCTGCTAAGATAAAATCTACAGAGATTAGAAAGAGAAAAAGGGCAAAGGAAAGCTATGGTGACTCTCAAAAGATGGTTCAAGTCTCTGTTGGAGGGGAGAATCAGACCATTGATTGGGCTATACGACTAACAGTCGCTCTGAACATTGAAAAAGCATTAGATCACTGCAGCAGCGAATGCCGCCCATTGTACCATGACTTAAATGCATATATGGTTCTCTTTGATAAGAGTTGGCTTCATTTCAGTCCAGTAACTCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0016787 GO:0016822 GO:0016823 GO:0018773 GO:0034545 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0046914

KEGG Pathways

Metabolic & Signaling

ko00350 ko01100 ko01120 map00350 map01100 map01120

Pfam Domains

Protein Families

FAA_hydrolase

Protein Analysis

142

Amino Acids

15.72

Weight (kDa)

6.28

Isoelectric Point (pI)

53.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAA_hydrolase	PF01557	5 - 110	3.9e-13	Fumarylacetoacetate (FAA) hydrolase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002365)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16700 AT3G16700 AT3G16700 AT3G16700 AT4G15940
fragaria_vesca	FvH4_4g18580 FvH4_4g18580
malus_domestica	MD04G1020300.v1.1
prunus_persica	Prupe.1G124800_v2.0.a1 Prupe.1G125100_v2.0.a1
pyrus_communis	pycom10g01710 pycom10g01730 pycom16g13990
rosa_chinensis	RchiOBHm_Chr0c32g0501491 RchiOBHm_Chr4g0423181 RchiOBHm_Chr4g0423191
rosa_laevigata	RLG00000007578 RLG00000033195
rosa_multiflora	Rmu_sc0000580.1_g000006 Rmu_sc0003368.1_g000036 Rmu_sc0007269.1_g000013 Rmu_sc0007782.1_g000002 Rmu_sc0008829.1_g000004
rosa_roxburghii	Rroxscaffold_5G00365340 Rroxscaffold_5G00365370
rosa_rugosa	Rorug02G0398700 Rorug04G0185000 Rorug04G0185100
rosa_samantha	Rh2AG299600 Rh4AG244500 Rh4AG247800 Rh4BG249700 Rh4CG258800 Rh4DG244500 Rh5AG208600 Rh5AG217600 Rh5AG294800 Rh5BG129400 Rh5BG217400 Rh5DG211400 Rh6AG307700
rosa_wichuraiana	Rw4G021060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	55, 279
AciI	CCGC	6 cut(s)	9, 59, 92, 102, 119, 346
AcsI	RAATTY	1 cut(s)	156
AfaI	GTAC	1 cut(s)	356
AfiI	CCNNNNNNNGG	2 cut(s)	55, 279
AgsI	TTSAA	3 cut(s)	161, 245, 314
AhdI	GACNNNNNGTC	1 cut(s)	296
AluBI	AGCT	2 cut(s)	80, 219
AluI	AGCT	2 cut(s)	80, 219
Alw26I	GTCTC	1 cut(s)	253
ApeKI	GCWGC	2 cut(s)	333, 336
ApoI	RAATTY	1 cut(s)	156
AspS9I	GGNCC	1 cut(s)	46
AsuHPI	GGTGA	3 cut(s)	20, 73, 236
AvaII	GGWCC	1 cut(s)	46
BbvI	GCAGC	2 cut(s)	345, 348
BccI	CCATC	1 cut(s)	232
BceAI	ACGGC	1 cut(s)	22
BclI	TGATCA	1 cut(s)	63
BcoDI	GTCTC	1 cut(s)	253
BfmI	CTRYAG	2 cut(s)	183, 331
BisI	GCNGC	4 cut(s)	10, 334, 337, 346
BlsI	GCNGC	4 cut(s)	11, 335, 338, 347
Bme18I	GGWCC	1 cut(s)	46
BmeRI	GACNNNNNGTC	1 cut(s)	296
BmgT120I	GGNCC	1 cut(s)	46
BmsI	GCATC	1 cut(s)	173
BsaJI	CCNNGG	1 cut(s)	105
Bsc4I	CCNNNNNNNGG	2 cut(s)	55, 279
Bse1I	ACTGG	1 cut(s)	413
Bse3DI	GCAATG	1 cut(s)	114
BseDI	CCNNGG	1 cut(s)	105
BseLI	CCNNNNNNNGG	2 cut(s)	55, 279
BseMI	GCAATG	1 cut(s)	114
BseMII	CTCAG	1 cut(s)	86
BseNI	ACTGG	1 cut(s)	413
BseXI	GCAGC	2 cut(s)	345, 348
Bsh1285I	CGRYCG	1 cut(s)	18
BsiEI	CGRYCG	1 cut(s)	18
BslFI	GGGAC	1 cut(s)	8
BslI	CCNNNNNNNGG	2 cut(s)	55, 279
BsmAI	GTCTC	1 cut(s)	253
BsmFI	GGGAC	1 cut(s)	8
BsmI	GAATGC	1 cut(s)	347
Bsp143I	GATC	3 cut(s)	15, 63, 325
Bsp19I	CCATGG	1 cut(s)	105
BspACI	CCGC	6 cut(s)	9, 59, 92, 102, 119, 346
BspCNI	CTCAG	1 cut(s)	85
BspMAI	CTGCAG	1 cut(s)	335
BsrDI	GCAATG	1 cut(s)	114
BsrI	ACTGG	1 cut(s)	413
BssECI	CCNNGG	1 cut(s)	105
BssMI	GATC	3 cut(s)	15, 63, 325
BssT1I	CCWWGG	1 cut(s)	105
Bst4CI	ACNGT	1 cut(s)	298
BstAPI	GCANNNNNTGC	1 cut(s)	342
BstDEI	CTNAG	2 cut(s)	72, 171
BstDSI	CCRYGG	1 cut(s)	105
BstKTI	GATC	3 cut(s)	18, 66, 328
BstMAI	GTCTC	1 cut(s)	253
BstMBI	GATC	3 cut(s)	15, 63, 325
BstMCI	CGRYCG	1 cut(s)	18
BstMWI	GCNNNNNNNGC	3 cut(s)	125, 342, 345
BstSFI	CTRYAG	2 cut(s)	183, 331
BstV1I	GCAGC	2 cut(s)	345, 348
BstXI	CCANNNNNNTGG	1 cut(s)	106
BtgI	CCRYGG	1 cut(s)	105
BtsI	GCAGTG	1 cut(s)	328
BtsIMutI	CAGTG	1 cut(s)	328
Cfr13I	GGNCC	1 cut(s)	46
Csp6I	GTAC	1 cut(s)	355
CviAII	CATG	3 cut(s)	50, 106, 359
CviJI	RGCY	4 cut(s)	80, 219, 284, 400
CviKI_1	RGCY	4 cut(s)	80, 219, 284, 400
CviQI	GTAC	1 cut(s)	355
DdeI	CTNAG	2 cut(s)	72, 171
DpnI	GATC	3 cut(s)	17, 65, 327
DpnII	GATC	3 cut(s)	15, 63, 325
DriI	GACNNNNNGTC	1 cut(s)	296
Eam1105I	GACNNNNNGTC	1 cut(s)	296
Eco130I	CCWWGG	1 cut(s)	105
Eco47I	GGWCC	1 cut(s)	46
EcoT14I	CCWWGG	1 cut(s)	105
EcoT22I	ATGCAT	1 cut(s)	373
ErhI	CCWWGG	1 cut(s)	105
FaeI	CATG	3 cut(s)	53, 109, 362
FaqI	GGGAC	1 cut(s)	8
FatI	CATG	3 cut(s)	49, 105, 358
FauI	CCCGC	1 cut(s)	112
FbaI	TGATCA	1 cut(s)	63
Fnu4HI	GCNGC	4 cut(s)	10, 334, 337, 346
Fsp4HI	GCNGC	4 cut(s)	10, 334, 337, 346
GluI	GCNGC	4 cut(s)	10, 334, 337, 346
Hin1II	CATG	3 cut(s)	53, 109, 362
HinfI	GANTC	2 cut(s)	227, 265
HphI	GGTGA	3 cut(s)	20, 73, 236
Hpy188I	TCNGA	3 cut(s)	75, 270, 306
Hpy188III	TCNNGA	1 cut(s)	83
HpyCH4III	ACNGT	1 cut(s)	298
HpyCH4V	TGCA	3 cut(s)	128, 333, 371
HpyF10VI	GCNNNNNNNGC	3 cut(s)	125, 342, 345
HpyF3I	CTNAG	2 cut(s)	72, 171
Hsp92II	CATG	3 cut(s)	53, 109, 362
Ksp22I	TGATCA	1 cut(s)	63
Kzo9I	GATC	3 cut(s)	15, 63, 325
LpnPI	CCDG	1 cut(s)	163
Lsp1109I	GCAGC	2 cut(s)	345, 348
LweI	GCATC	1 cut(s)	173
MaeIII	GTNAC	2 cut(s)	224, 415
MalI	GATC	3 cut(s)	17, 65, 327
MboI	GATC	3 cut(s)	15, 63, 325
MluCI	AATT	1 cut(s)	156
MlyI	GAGTC	1 cut(s)	221
MmeI	TCCRAC	1 cut(s)	235
MnlI	CCTC	4 cut(s)	36, 42, 46, 251
Mph1103I	ATGCAT	1 cut(s)	373
MseI	TTAA	1 cut(s)	365
MslI	CAYNNNNRTG	1 cut(s)	104
Mva1269I	GAATGC	1 cut(s)	347
MwoI	GCNNNNNNNGC	3 cut(s)	125, 342, 345
NcoI	CCATGG	1 cut(s)	105
NdeII	GATC	3 cut(s)	15, 63, 325
NlaIII	CATG	3 cut(s)	53, 109, 362
NmuCI	GTSAC	1 cut(s)	224
NsiI	ATGCAT	1 cut(s)	373
PctI	GAATGC	1 cut(s)	347
PfeI	GAWTC	1 cut(s)	265
PflMI	CCANNNNNTGG	2 cut(s)	55, 279
PkrI	GCNGC	4 cut(s)	11, 335, 338, 347
Ple19I	CGATCG	1 cut(s)	18
PleI	GAGTC	1 cut(s)	221
PpsI	GAGTC	1 cut(s)	221
PspPI	GGNCC	1 cut(s)	46
PstI	CTGCAG	1 cut(s)	335
PvuI	CGATCG	1 cut(s)	18
RsaI	GTAC	1 cut(s)	356
RsaNI	GTAC	1 cut(s)	355
RseI	CAYNNNNRTG	1 cut(s)	104
SaqAI	TTAA	1 cut(s)	365
SatI	GCNGC	4 cut(s)	10, 334, 337, 346
Sau3AI	GATC	3 cut(s)	15, 63, 325
Sau96I	GGNCC	1 cut(s)	46
SchI	GAGTC	1 cut(s)	221
SetI	ASST	3 cut(s)	57, 82, 221
SfaNI	GCATC	1 cut(s)	173
SfcI	CTRYAG	2 cut(s)	183, 331
SinI	GGWCC	1 cut(s)	46
SmiMI	CAYNNNNRTG	1 cut(s)	104
Sse9I	AATT	1 cut(s)	156
SsiI	CCGC	6 cut(s)	9, 59, 92, 102, 119, 346
StyI	CCWWGG	1 cut(s)	105
TaaI	ACNGT	1 cut(s)	298
TaqI	TCGA	1 cut(s)	18
TasI	AATT	1 cut(s)	156
TauI	GCSGC	2 cut(s)	12, 348
TfiI	GAWTC	1 cut(s)	265
Tru1I	TTAA	1 cut(s)	365
Tru9I	TTAA	1 cut(s)	365
TscAI	CASTG	1 cut(s)	335
TseFI	GTSAC	1 cut(s)	224
TseI	GCWGC	2 cut(s)	333, 336
Tsp45I	GTSAC	1 cut(s)	224
TspDTI	ATGAA	2 cut(s)	17, 392
TspRI	CASTG	1 cut(s)	335
Van91I	CCANNNNNTGG	2 cut(s)	55, 279
VpaK11BI	GGWCC	1 cut(s)	46
XapI	RAATTY	1 cut(s)	156
Zsp2I	ATGCAT	1 cut(s)	373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.