Rroxscaffold_5G00365370

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002365
Physical Location & Seq
Forward (+)
46926963 .. 46928692
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365370.1

Sequence Viewer

Length: 570 bp
ATGGCAAGCAGGCTTTTACAAGTAGGCACTAAGATCATCGGAGTCGGCAGAAACTACGCCGTCCCCAAGGAGTCTGTGCTGTTTCTCAAACCGACGTCGTCTTACTTGGAAAACGGAGGCACGATCGAAATCCCTCACCCTCTGCAGTCGTTGGACTATGAGGTGGAGCTGGCCGTCATCATATCCAAGAAAGCTCGCGATGTTCCCCAGGCCAAGGCCATGGATTACGTTGGTGGTTATGCACTTGCTCTTGATATGACTGCCAGAGAGATTCAAGCTACTGCTAAGTTGTCCCTAGCAACGGTGCCAGACCCTGACGATTTGGAACTTTGGTTGAAGGTAGATGGAGAAGTGAGGCAAAAAGGTTCAACCAAGGATATGATATTTAAAATCCCATTTCTCATTAGCCACATAAGTTCCATTATGACACTTCTGGAAGGAGATGTAATACTAACAGGTACTCCCAAAGGTGTTGGTCCAGTAAAAGCAGGCCAAAAGATAACCGCGGGAATAACAAACCTTGTGGATGTACAATTCAATGCTGACAAATGGAGGCAAGGAAGTTCTTGA

Protein Analysis

189

Amino Acids

20.45

Weight (kDa)

6.74

Isoelectric Point (pI)

26.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 20 - 176 2.1e-43 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 98
AccB1I GGYRCC 1 cut(s) 304
AccII CGCG 2 cut(s) 198, 506
AciI CCGC 2 cut(s) 504, 506
AcoI YGGCCR 1 cut(s) 171
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 2 cut(s) 460, 531
AfiI CCNNNNNNNGG 2 cut(s) 214, 301
AgsI TTSAA 4 cut(s) 275, 337, 369, 538
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 3 cut(s) 169, 194, 278
AluI AGCT 3 cut(s) 169, 194, 278
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 4 cut(s) 171, 210, 216, 490
AspS9I GGNCC 1 cut(s) 476
AsuHPI GGTGA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 304
BccI CCATC 1 cut(s) 338
BceAI ACGGC 2 cut(s) 44, 158
BciT130I CCWGG 1 cut(s) 209
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 143
Bme1390I CCNGG 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 6 cut(s) 66, 207, 213, 219, 372, 504
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 301
Bse1I ACTGG 1 cut(s) 479
Bse8I GATNNNNATC 1 cut(s) 128
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 6 cut(s) 66, 207, 213, 219, 372, 504
BseGI GGATG 1 cut(s) 532
BseJI GATNNNNATC 1 cut(s) 128
BseLI CCNNNNNNNGG 2 cut(s) 214, 301
BseNI ACTGG 1 cut(s) 479
Bsh1236I CGCG 2 cut(s) 198, 506
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 4 cut(s) 173, 212, 218, 492
BshNI GGYRCC 1 cut(s) 304
BsiEI CGRYCG 1 cut(s) 126
BslFI GGGAC 2 cut(s) 47, 277
BslI CCNNNNNNNGG 2 cut(s) 214, 301
BsmFI GGGAC 2 cut(s) 47, 277
BsnI GGCC 4 cut(s) 173, 212, 218, 492
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 2 cut(s) 33, 123
Bsp19I CCATGG 1 cut(s) 219
Bsp68I TCGCGA 1 cut(s) 198
BspACI CCGC 2 cut(s) 504, 506
BspANI GGCC 4 cut(s) 173, 212, 218, 492
BspFNI CGCG 2 cut(s) 198, 506
BspLI GGNNCC 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 147
BspT107I GGYRCC 1 cut(s) 304
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 6 cut(s) 66, 207, 213, 219, 372, 504
BssMI GATC 2 cut(s) 33, 123
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 4 cut(s) 66, 213, 219, 372
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 304
BstACI GRCGYC 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 5 cut(s) 7, 11, 171, 196, 490
BstDEI CTNAG 2 cut(s) 30, 285
BstDSI CCRYGG 2 cut(s) 219, 504
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 2 cut(s) 198, 506
BstKTI GATC 2 cut(s) 36, 126
BstMBI GATC 2 cut(s) 33, 123
BstMCI CGRYCG 1 cut(s) 126
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 1 cut(s) 207
BstSFI CTRYAG 1 cut(s) 143
BstUI CGCG 2 cut(s) 198, 506
BstXI CCANNNNNNTGG 1 cut(s) 220
BsuRI GGCC 4 cut(s) 173, 212, 218, 492
BtgI CCRYGG 2 cut(s) 219, 504
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 1 cut(s) 532
BtuMI TCGCGA 1 cut(s) 198
Cac8I GCNNGC 5 cut(s) 7, 11, 171, 196, 490
CaiI CAGNNNCTG 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 476
Cfr42I CCGCGG 1 cut(s) 507
Csp6I GTAC 2 cut(s) 459, 530
CspCI CAANNNNNGTGG 2 cut(s) 504, 539
CviAII CATG 1 cut(s) 220
CviJI RGCY 9 cut(s) 13, 169, 173, 194, 212, 218, 278, 408, 492
CviKI_1 RGCY 9 cut(s) 13, 169, 173, 194, 212, 218, 278, 408, 492
CviQI GTAC 2 cut(s) 459, 530
DdeI CTNAG 2 cut(s) 30, 285
DpnI GATC 2 cut(s) 35, 125
DpnII GATC 2 cut(s) 33, 123
DraI TTTAAA 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 171
Eco130I CCWWGG 4 cut(s) 66, 213, 219, 372
Eco47I GGWCC 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 207
EcoT14I CCWWGG 4 cut(s) 66, 213, 219, 372
ErhI CCWWGG 4 cut(s) 66, 213, 219, 372
FaeI CATG 1 cut(s) 223
FaiI YATR 8 cut(s) 159, 182, 221, 240, 257, 380, 413, 425
FaqI GGGAC 2 cut(s) 47, 277
FatI CATG 1 cut(s) 219
FauI CCCGC 1 cut(s) 499
FokI GGATG 1 cut(s) 539
FspBI CTAG 1 cut(s) 296
HaeIII GGCC 4 cut(s) 173, 212, 218, 492
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 223
HinfI GANTC 3 cut(s) 42, 71, 271
HphI GGTGA 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 4 cut(s) 197, 251, 434, 567
Hpy99I CGWCG 2 cut(s) 97, 100
HpyAV CCTTC 2 cut(s) 331, 431
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 2 cut(s) 95, 228
HpyCH4V TGCA 2 cut(s) 145, 242
HpyF3I CTNAG 2 cut(s) 30, 285
HpySE526I ACGT 2 cut(s) 95, 228
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 223
KspI CCGCGG 1 cut(s) 507
Kzo9I GATC 2 cut(s) 33, 123
LmnI GCTCC 1 cut(s) 166
MaeI CTAG 1 cut(s) 296
MaeII ACGT 2 cut(s) 95, 228
MalI GATC 2 cut(s) 35, 125
MboI GATC 2 cut(s) 33, 123
MluCI AATT 1 cut(s) 533
MlyI GAGTC 2 cut(s) 51, 80
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 6 cut(s) 110, 144, 150, 154, 348, 546
MseI TTAA 1 cut(s) 387
MspA1I CMGCKG 1 cut(s) 506
MspR9I CCNGG 1 cut(s) 209
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 2 cut(s) 198, 506
NcoI CCATGG 1 cut(s) 219
NdeII GATC 2 cut(s) 33, 123
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 1 cut(s) 306
NruI TCGCGA 1 cut(s) 198
PfeI GAWTC 1 cut(s) 271
PflFI GACNNNGTC 1 cut(s) 97
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 2 cut(s) 50, 79
PpsI GAGTC 2 cut(s) 50, 79
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 1 cut(s) 476
PstI CTGCAG 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 314
PsyI GACNNNGTC 1 cut(s) 97
PvuI CGATCG 1 cut(s) 126
RruI TCGCGA 1 cut(s) 198
RsaI GTAC 2 cut(s) 460, 531
RsaNI GTAC 2 cut(s) 459, 530
SacII CCGCGG 1 cut(s) 507
SaqAI TTAA 1 cut(s) 387
Sau3AI GATC 2 cut(s) 33, 123
Sau96I GGNCC 1 cut(s) 476
SchI GAGTC 2 cut(s) 51, 80
ScrFI CCNGG 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 143
Sfr303I CCGCGG 1 cut(s) 507
SgrBI CCGCGG 1 cut(s) 507
SinI GGWCC 1 cut(s) 476
Sse9I AATT 1 cut(s) 533
SsiI CCGC 2 cut(s) 504, 506
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 1 cut(s) 207
StyI CCWWGG 4 cut(s) 66, 213, 219, 372
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 2 cut(s) 98, 231
TaqI TCGA 1 cut(s) 126
TasI AATT 1 cut(s) 533
TatI WGTACW 1 cut(s) 529
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TspGWI ACGGA 1 cut(s) 129
Tth111I GACNNNGTC 1 cut(s) 97
VpaK11BI GGWCC 1 cut(s) 476
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.