Rh5AG294800

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002365
Physical Location & Seq
Forward (+)
40926438 .. 40926804
367 bp
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UTR
Exon/CDS
Intron
Rh5AG294800.1

Sequence Viewer

Length: 249 bp
ATGAAGGAGCTTGTGCTTTTTCTCAAACCGACGTCGTCTTACTTGGAAAACAGAGGCACCATCGAAATCCCTCACCCTCTGGAGTCGTTGGACCATGAGGTGGAGCTGGCTGTCATCATATCCAAGAAAGCTCGCGATGTTCCCCAGGCTAAGGCCATGGATTACGTTAGTGGTTATGCACTTGCTCTTGATATGACTGCCAGAGAGATTCAAGCTACTGCTAAGGTAATCATCATGTTTGTGACGTAA

Protein Analysis

82

Amino Acids

9.16

Weight (kDa)

5.58

Isoelectric Point (pI)

24.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 4 - 75 1.4e-20 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 100
AccII CGCG 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 100, 151
AgsI TTSAA 1 cut(s) 212
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 4 cut(s) 10, 106, 131, 215
AluI AGCT 4 cut(s) 10, 106, 131, 215
AoxI GGCC 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 91
AsuHPI GGTGA 1 cut(s) 65
AvaII GGWCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 56
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 146
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 58
BmrFI CCNGG 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 2 cut(s) 150, 222
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 144, 156
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 151
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 144, 156
BseLI CCNNNNNNNGG 2 cut(s) 100, 151
Bsh1236I CGCG 1 cut(s) 135
BshFI GGCC 1 cut(s) 155
BshNI GGYRCC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 100, 151
BsnI GGCC 1 cut(s) 155
Bsp19I CCATGG 1 cut(s) 156
Bsp68I TCGCGA 1 cut(s) 135
BspANI GGCC 1 cut(s) 155
BspFNI CGCG 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 58
BspT107I GGYRCC 1 cut(s) 56
BssECI CCNNGG 2 cut(s) 144, 156
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 146
BstACI GRCGYC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 108, 133
BstDEI CTNAG 2 cut(s) 150, 222
BstDSI CCRYGG 1 cut(s) 156
BstFNI CGCG 1 cut(s) 135
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 1 cut(s) 135
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 150
BtuMI TCGCGA 1 cut(s) 135
Cac8I GCNNGC 2 cut(s) 108, 133
Cfr13I GGNCC 1 cut(s) 91
CviAII CATG 3 cut(s) 95, 157, 235
CviJI RGCY 7 cut(s) 10, 106, 110, 131, 149, 155, 215
CviKI_1 RGCY 7 cut(s) 10, 106, 110, 131, 149, 155, 215
DdeI CTNAG 2 cut(s) 150, 222
Eco130I CCWWGG 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 156
FaeI CATG 3 cut(s) 98, 160, 238
FaiI YATR 6 cut(s) 96, 119, 158, 177, 194, 236
FatI CATG 3 cut(s) 94, 156, 234
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 1 cut(s) 155
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 3 cut(s) 98, 160, 238
HinfI GANTC 2 cut(s) 83, 208
HphI GGTGA 1 cut(s) 65
Hpy188III TCNNGA 3 cut(s) 80, 134, 188
Hpy99I CGWCG 2 cut(s) 34, 37
HpyCH4IV ACGT 3 cut(s) 32, 165, 245
HpyCH4V TGCA 1 cut(s) 179
HpyF3I CTNAG 2 cut(s) 150, 222
HpySE526I ACGT 3 cut(s) 32, 165, 245
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 3 cut(s) 98, 160, 238
LmnI GCTCC 2 cut(s) 7, 103
LpnPI CCDG 5 cut(s) 65, 92, 131, 158, 214
MaeII ACGT 3 cut(s) 32, 165, 245
MaeIII GTNAC 1 cut(s) 241
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 69
MnlI CCTC 4 cut(s) 47, 81, 87, 91
MslI CAYNNNNRTG 1 cut(s) 239
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 135
NcoI CCATGG 1 cut(s) 156
NlaIII CATG 3 cut(s) 98, 160, 238
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 241
NruI TCGCGA 1 cut(s) 135
PfeI GAWTC 1 cut(s) 208
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 100
PleI GAGTC 1 cut(s) 91
PpsI GAGTC 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 58
PspPI GGNCC 1 cut(s) 91
PsyI GACNNNGTC 1 cut(s) 34
RruI TCGCGA 1 cut(s) 135
RseI CAYNNNNRTG 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 146
SetI ASST 9 cut(s) 12, 35, 102, 108, 133, 168, 217, 228, 248
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 239
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 156
TaiI ACGT 3 cut(s) 35, 168, 248
TaqI TCGA 1 cut(s) 63
TfiI GAWTC 1 cut(s) 208
TseFI GTSAC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 91
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.