Rh5BG217400

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002365
Physical Location & Seq
Forward (+)
25114441 .. 25115917
1477 bp
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UTR
Exon/CDS
Intron
Rh5BG217400.1

Sequence Viewer

Length: 243 bp
ATGAGGTGGAGGCGTGGAGCTGCTGTCATCATATCCAAGAAAGCTCGCGATGTTCCTCAGGCCAAGGCCATGGATTACGTTGGTGGTTATGCACTTGCTCTTGATATTACTGACAGAGAGATTCAAGCTACTGCTAAGATTGAGGTTGGGCAACTGCCACCCCCTGAATCCGCCCCTGCCGGCGAAGACGCTCGACGGCAAGGCCCAGCGGCTATTCGGACTCCGATCGACACAAGTCCTTAG

Protein Analysis

80

Amino Acids

8.58

Weight (kDa)

9.3

Isoelectric Point (pI)

66.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 8 - 49 1.4e-09 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 48
AciI CCGC 2 cut(s) 171, 209
AgsI TTSAA 1 cut(s) 125
AluBI AGCT 3 cut(s) 20, 44, 128
AluI AGCT 3 cut(s) 20, 44, 128
AoxI GGCC 3 cut(s) 60, 66, 202
ApeKI GCWGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 57
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 7
BceAI ACGGC 1 cut(s) 212
BisI GCNGC 2 cut(s) 21, 210
BlsI GCNGC 2 cut(s) 22, 211
BmgT120I GGNCC 1 cut(s) 203
BoxI GACNNNNGTC 1 cut(s) 234
BpiI GAAGAC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 63, 69
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bse118I RCCGGY 1 cut(s) 179
Bse21I CCTNAGG 1 cut(s) 57
BseDI CCNNGG 2 cut(s) 63, 69
BseMII CTCAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 7
BseYI CCCAGC 1 cut(s) 205
Bsh1236I CGCG 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 3 cut(s) 62, 68, 204
BsiEI CGRYCG 1 cut(s) 228
BsiSI CCGG 1 cut(s) 180
BsnI GGCC 3 cut(s) 62, 68, 204
Bsp143I GATC 1 cut(s) 225
Bsp19I CCATGG 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 48
BspACI CCGC 2 cut(s) 171, 209
BspANI GGCC 3 cut(s) 62, 68, 204
BspCNI CTCAG 1 cut(s) 70
BspFNI CGCG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 179
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 63, 69
BssMI GATC 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 63, 69
BstC8I GCNNGC 2 cut(s) 46, 181
BstDEI CTNAG 3 cut(s) 57, 135, 240
BstDSI CCRYGG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 48
BstKTI GATC 1 cut(s) 228
BstMBI GATC 1 cut(s) 225
BstMCI CGRYCG 1 cut(s) 228
BstPAI GACNNNNGTC 1 cut(s) 234
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 70
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 62, 68, 204
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 63
BtuMI TCGCGA 1 cut(s) 48
Cac8I GCNNGC 2 cut(s) 46, 181
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 197
CviAII CATG 1 cut(s) 70
CviJI RGCY 7 cut(s) 20, 44, 62, 68, 128, 204, 212
CviKI_1 RGCY 7 cut(s) 20, 44, 62, 68, 128, 204, 212
DdeI CTNAG 3 cut(s) 57, 135, 240
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 63, 69
Eco81I CCTNAGG 1 cut(s) 57
EcoT14I CCWWGG 2 cut(s) 63, 69
ErhI CCWWGG 2 cut(s) 63, 69
FaeI CATG 1 cut(s) 73
FaiI YATR 3 cut(s) 32, 71, 90
FatI CATG 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 21, 210
Fsp4HI GCNGC 2 cut(s) 21, 210
GluI GCNGC 2 cut(s) 21, 210
GsaI CCCAGC 1 cut(s) 209
HaeIII GGCC 3 cut(s) 62, 68, 204
HapII CCGG 1 cut(s) 180
HgaI GACGC 1 cut(s) 197
Hin1II CATG 1 cut(s) 73
HinfI GANTC 3 cut(s) 121, 167, 220
HpaII CCGG 1 cut(s) 180
Hpy188I TCNGA 2 cut(s) 219, 225
Hpy188III TCNNGA 2 cut(s) 47, 101
Hpy99I CGWCG 1 cut(s) 198
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 1 cut(s) 92
HpyF3I CTNAG 3 cut(s) 57, 135, 240
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 1 cut(s) 73
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 17
LpnPI CCDG 5 cut(s) 44, 177, 189, 193, 219
Lsp1109I GCAGC 1 cut(s) 7
MaeII ACGT 1 cut(s) 78
MalI GATC 1 cut(s) 227
MboI GATC 1 cut(s) 225
MboII GAAGA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 214
MnlI CCTC 3 cut(s) 3, 66, 136
MroNI GCCGGC 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 1 cut(s) 180
MvnI CGCG 1 cut(s) 48
NaeI GCCGGC 1 cut(s) 181
NcoI CCATGG 1 cut(s) 69
NdeII GATC 1 cut(s) 225
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 1 cut(s) 73
NruI TCGCGA 1 cut(s) 48
PdiI GCCGGC 1 cut(s) 181
PfeI GAWTC 2 cut(s) 121, 167
PkrI GCNGC 2 cut(s) 22, 211
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 203
PvuI CGATCG 1 cut(s) 228
RruI TCGCGA 1 cut(s) 48
SatI GCNGC 2 cut(s) 21, 210
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 1 cut(s) 214
SetI ASST 6 cut(s) 8, 22, 46, 81, 130, 147
SsiI CCGC 2 cut(s) 171, 209
StyI CCWWGG 2 cut(s) 63, 69
TaiI ACGT 1 cut(s) 81
TaqI TCGA 2 cut(s) 193, 228
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 2 cut(s) 121, 167
TseI GCWGC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.