RLG00000007578

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002365
Physical Location & Seq
Reverse (-)
18495164 .. 18496939
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007578

Sequence Viewer

Length: 660 bp
ATGGCAAGCAGGCTTTTACAAGTCGGCACTAAGATCATCGGAGTCGGCAGAAACTACGCCGCCCACGCCAAAGAACTCGGCAACGCCGTCCCCAAGGAGCCTGTGCTTTTCCTCAAACCGACGTCGTCTTACTTGGAAAACGGAGGCACGATCGAAATCCCTCACCCTCTGCAGTCGTTGGACTATGAGGTGGAGCTGGCTGTCATCATATCCAAGAAAGCTCGCGATGTTCCCCAGGCCAAGGCCATGGATTACGTTGGTGGTTATGCGCTTGCTCTTGATATGACTGCCAGAGAGATTCAAGCTACTGCTAAGTCTGCAGGTCTTCCATGGACCATAGCAAAAGGGCAGGACACCTTCACACCAATCAGTTCTGTGTTGTCCCTAGCAACGGTTCCAGACCCTGACGATTTGGAACTTTGGTTGAAGGTAGATGGAGAAGTGAGGCAAAAAGGCTCAACCAAGGATATGATATTTAAAATCCCATTTCTCATCAGCCACATAAGTTCCATTATGACACTTCTGGAAGGAGATGTAATACTAACAGGTACTCCCAAAGGTGTTGGTCCAGTAAAAGCGGGCCAACAGATAACCGCAGGAATAACAAACCTTGTGGATGTACAATTCAATGTTGACAAACGGAGGCAAGGAAGTTCTTGA

Protein Analysis

220

Amino Acids

23.51

Weight (kDa)

6.84

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 11 - 211 1.9e-64 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 311
AccII CGCG 1 cut(s) 225
AciI CCGC 3 cut(s) 60, 578, 594
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 2 cut(s) 550, 621
AfiI CCNNNNNNNGG 2 cut(s) 241, 391
AgsI TTSAA 3 cut(s) 302, 427, 628
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 3 cut(s) 196, 221, 305
AluI AGCT 3 cut(s) 196, 221, 305
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 3 cut(s) 237, 243, 580
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 333, 566, 580
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 2 cut(s) 333, 566
BbsI GAAGAC 1 cut(s) 317
BccI CCATC 1 cut(s) 428
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 236
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 2 cut(s) 170, 318
BfuAI ACCTGC 1 cut(s) 311
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 2 cut(s) 333, 566
BmgT120I GGNCC 3 cut(s) 333, 566, 580
BmiI GGNNCC 2 cut(s) 99, 396
BmrFI CCNGG 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 6 cut(s) 93, 234, 240, 246, 329, 462
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 391
Bse1I ACTGG 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 6 cut(s) 93, 234, 240, 246, 329, 462
BseGI GGATG 1 cut(s) 622
BseJI GATNNNNATC 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 241, 391
BseNI ACTGG 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 3 cut(s) 239, 245, 582
BsiEI CGRYCG 1 cut(s) 153
BslFI GGGAC 2 cut(s) 74, 367
BslI CCNNNNNNNGG 2 cut(s) 241, 391
BsmFI GGGAC 2 cut(s) 74, 367
BsnI GGCC 3 cut(s) 239, 245, 582
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 2 cut(s) 33, 150
Bsp19I CCATGG 2 cut(s) 246, 329
Bsp68I TCGCGA 1 cut(s) 225
BspACI CCGC 3 cut(s) 60, 578, 594
BspANI GGCC 3 cut(s) 239, 245, 582
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 99, 396
BspMAI CTGCAG 2 cut(s) 174, 322
BspMI ACCTGC 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 1 cut(s) 569
BssECI CCNNGG 6 cut(s) 93, 234, 240, 246, 329, 462
BssMI GATC 2 cut(s) 33, 150
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 5 cut(s) 93, 240, 246, 329, 462
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 394
BstACI GRCGYC 1 cut(s) 122
BstAUI TGTACA 1 cut(s) 619
BstC8I GCNNGC 6 cut(s) 7, 11, 198, 223, 273, 580
BstDEI CTNAG 2 cut(s) 30, 312
BstDSI CCRYGG 2 cut(s) 246, 329
BstF5I GGATG 1 cut(s) 622
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 2 cut(s) 36, 153
BstMBI GATC 2 cut(s) 33, 150
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 65, 317
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 2 cut(s) 170, 318
BstUI CGCG 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 317
BstXI CCANNNNNNTGG 1 cut(s) 247
BsuRI GGCC 3 cut(s) 239, 245, 582
BtgI CCRYGG 2 cut(s) 246, 329
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 622
BtuMI TCGCGA 1 cut(s) 225
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 6 cut(s) 7, 11, 198, 223, 273, 580
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 3 cut(s) 333, 566, 580
Csp6I GTAC 2 cut(s) 549, 620
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 2 cut(s) 247, 330
CviQI GTAC 2 cut(s) 549, 620
DdeI CTNAG 2 cut(s) 30, 312
DpnI GATC 2 cut(s) 35, 152
DpnII GATC 2 cut(s) 33, 150
DraI TTTAAA 1 cut(s) 478
Eco130I CCWWGG 5 cut(s) 93, 240, 246, 329, 462
Eco47I GGWCC 2 cut(s) 333, 566
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 5 cut(s) 93, 240, 246, 329, 462
ErhI CCWWGG 5 cut(s) 93, 240, 246, 329, 462
FaeI CATG 2 cut(s) 250, 333
FaqI GGGAC 2 cut(s) 74, 367
FatI CATG 2 cut(s) 246, 329
FauI CCCGC 1 cut(s) 571
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 1 cut(s) 60
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 270
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 3 cut(s) 239, 245, 582
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 2 cut(s) 250, 333
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 2 cut(s) 42, 298
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 5 cut(s) 224, 278, 398, 524, 657
Hpy8I GTNNAC 1 cut(s) 634
Hpy99I CGWCG 2 cut(s) 124, 127
HpyAV CCTTC 3 cut(s) 367, 421, 521
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 2 cut(s) 122, 255
HpyCH4V TGCA 2 cut(s) 172, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 317
HpyF3I CTNAG 2 cut(s) 30, 312
HpySE526I ACGT 2 cut(s) 122, 255
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 2 cut(s) 250, 333
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 2 cut(s) 33, 150
LmnI GCTCC 2 cut(s) 97, 193
MaeI CTAG 1 cut(s) 386
MaeII ACGT 2 cut(s) 122, 255
MalI GATC 2 cut(s) 35, 152
MboI GATC 2 cut(s) 33, 150
MboII GAAGA 1 cut(s) 317
MluCI AATT 1 cut(s) 623
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 7 cut(s) 122, 137, 171, 177, 181, 438, 636
MseI TTAA 1 cut(s) 477
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 65, 317
NcoI CCATGG 2 cut(s) 246, 329
NdeII GATC 2 cut(s) 33, 150
NlaIII CATG 2 cut(s) 250, 333
NlaIV GGNNCC 2 cut(s) 99, 396
NmeAIII GCCGAG 1 cut(s) 57
NruI TCGCGA 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 298
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 1 cut(s) 61
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 99, 396
PspPI GGNCC 3 cut(s) 333, 566, 580
PstI CTGCAG 2 cut(s) 174, 322
PstNI CAGNNNCTG 1 cut(s) 404
PsyI GACNNNGTC 1 cut(s) 124
PvuI CGATCG 1 cut(s) 153
RruI TCGCGA 1 cut(s) 225
RsaI GTAC 2 cut(s) 550, 621
RsaNI GTAC 2 cut(s) 549, 620
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 2 cut(s) 33, 150
Sau96I GGNCC 3 cut(s) 333, 566, 580
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 236
SfcI CTRYAG 2 cut(s) 170, 318
SinI GGWCC 2 cut(s) 333, 566
Sse9I AATT 1 cut(s) 623
SsiI CCGC 3 cut(s) 60, 578, 594
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 5 cut(s) 93, 240, 246, 329, 462
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 2 cut(s) 125, 258
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 623
TatI WGTACW 1 cut(s) 619
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TspGWI ACGGA 2 cut(s) 156, 655
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 2 cut(s) 333, 566
XspI CTAG 1 cut(s) 386
ZraI GACGTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.