Rh4BG249700

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002365
Physical Location & Seq
Forward (+)
41831072 .. 41833723
2652 bp
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UTR
Exon/CDS
Intron
Rh4BG249700.1

Sequence Viewer

Length: 555 bp
ATGGCAAGCAGGCTTTTACAAGTAGGCACTAAGATCATCGGAGTCGGCAGAAACTACGCCGCCCACGCCAAAGAGCTCGGCAACGCCGTCCCCAAGGAGCCTGTGCTTTTCCTCAAACCGACGTCGTCTTACTTGGAAAACGGAGGCACGATCGAAATCCCTCACCCTCTGCAGTCATTGGACTATGAGGTGGAGCTGGCCGTCATCATATCCAAGAAAGCTCGCGATGTTCCCCAGGCCAAGGCCATGGATTACGTTGGTGGTTATGCACTTGCTCTTGATATGACTGCCAGAGAGATTCAAGCTACTGCTAAGTCTGCAGGTCTTCCATGGACCATAGCAAAAGGGCAGGACACCTTCACACCAATCAGTTCTGTGTTGTCCCTAGCAACGGTGCCAGACCCTGACGATTTGGAACTTTGGTTGAAGGTAGATGGAGAAGTGAGGCAAAAAGGTTCAACCAAGGATATGATATTTAAAATCCCATTTCTCATTAGCCACATAAGTTCCATTATGACACTTCTGGAAGGAGATGTAATACTAACAGGTACATAG

Protein Analysis

184

Amino Acids

19.86

Weight (kDa)

5.61

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 11 - 184 2e-59 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 394
AccII CGCG 1 cut(s) 225
AciI CCGC 1 cut(s) 60
AcoI YGGCCR 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 241, 391
AgsI TTSAA 3 cut(s) 302, 427, 459
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 4 cut(s) 76, 196, 221, 305
AluI AGCT 4 cut(s) 76, 196, 221, 305
Alw21I GWGCWC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 3 cut(s) 198, 237, 243
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 333
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 1 cut(s) 428
BceAI ACGGC 2 cut(s) 71, 185
BciT130I CCWGG 1 cut(s) 236
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 2 cut(s) 170, 318
BfuAI ACCTGC 1 cut(s) 311
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 2 cut(s) 99, 396
BmrFI CCNGG 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 6 cut(s) 93, 234, 240, 246, 329, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 391
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 6 cut(s) 93, 234, 240, 246, 329, 462
BseJI GATNNNNATC 1 cut(s) 155
BseLI CCNNNNNNNGG 2 cut(s) 241, 391
Bsh1236I CGCG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 3 cut(s) 200, 239, 245
BshNI GGYRCC 1 cut(s) 394
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 78
BslFI GGGAC 2 cut(s) 74, 367
BslI CCNNNNNNNGG 2 cut(s) 241, 391
BsmFI GGGAC 2 cut(s) 74, 367
BsnI GGCC 3 cut(s) 200, 239, 245
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 2 cut(s) 33, 150
Bsp19I CCATGG 2 cut(s) 246, 329
Bsp68I TCGCGA 1 cut(s) 225
BspACI CCGC 1 cut(s) 60
BspANI GGCC 3 cut(s) 200, 239, 245
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 99, 396
BspMAI CTGCAG 2 cut(s) 174, 322
BspMI ACCTGC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 394
BssECI CCNNGG 6 cut(s) 93, 234, 240, 246, 329, 462
BssMI GATC 2 cut(s) 33, 150
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 5 cut(s) 93, 240, 246, 329, 462
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 394
BstACI GRCGYC 1 cut(s) 122
BstC8I GCNNGC 4 cut(s) 7, 11, 198, 223
BstDEI CTNAG 2 cut(s) 30, 312
BstDSI CCRYGG 2 cut(s) 246, 329
BstFNI CGCG 1 cut(s) 225
BstKTI GATC 2 cut(s) 36, 153
BstMBI GATC 2 cut(s) 33, 150
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 65, 317
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 2 cut(s) 170, 318
BstUI CGCG 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 317
BstXI CCANNNNNNTGG 1 cut(s) 247
BsuRI GGCC 3 cut(s) 200, 239, 245
BtgI CCRYGG 2 cut(s) 246, 329
BtgZI GCGATG 1 cut(s) 240
BtuMI TCGCGA 1 cut(s) 225
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 4 cut(s) 7, 11, 198, 223
CaiI CAGNNNCTG 1 cut(s) 404
Cfr13I GGNCC 1 cut(s) 333
Csp6I GTAC 1 cut(s) 549
CviAII CATG 2 cut(s) 247, 330
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 2 cut(s) 30, 312
DpnI GATC 2 cut(s) 35, 152
DpnII GATC 2 cut(s) 33, 150
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 5 cut(s) 93, 240, 246, 329, 462
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 5 cut(s) 93, 240, 246, 329, 462
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 5 cut(s) 93, 240, 246, 329, 462
FaeI CATG 2 cut(s) 250, 333
FaqI GGGAC 2 cut(s) 74, 367
FatI CATG 2 cut(s) 246, 329
Fnu4HI GCNGC 1 cut(s) 60
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 60
FspBI CTAG 1 cut(s) 386
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 3 cut(s) 200, 239, 245
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 2 cut(s) 250, 333
HinfI GANTC 2 cut(s) 42, 298
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 3 cut(s) 224, 278, 524
Hpy99I CGWCG 2 cut(s) 124, 127
HpyAV CCTTC 3 cut(s) 367, 421, 521
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 2 cut(s) 122, 255
HpyCH4V TGCA 3 cut(s) 172, 269, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 317
HpyF3I CTNAG 2 cut(s) 30, 312
HpySE526I ACGT 2 cut(s) 122, 255
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 2 cut(s) 250, 333
Kzo9I GATC 2 cut(s) 33, 150
LmnI GCTCC 2 cut(s) 97, 193
MaeI CTAG 1 cut(s) 386
MaeII ACGT 2 cut(s) 122, 255
MalI GATC 2 cut(s) 35, 152
MboI GATC 2 cut(s) 33, 150
MboII GAAGA 1 cut(s) 317
MhlI GDGCHC 1 cut(s) 78
MlyI GAGTC 1 cut(s) 51
MnlI CCTC 6 cut(s) 122, 137, 171, 177, 181, 438
MseI TTAA 1 cut(s) 477
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 65, 317
NcoI CCATGG 2 cut(s) 246, 329
NdeII GATC 2 cut(s) 33, 150
NlaIII CATG 2 cut(s) 250, 333
NlaIV GGNNCC 2 cut(s) 99, 396
NmeAIII GCCGAG 1 cut(s) 57
NruI TCGCGA 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 1 cut(s) 298
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 1 cut(s) 61
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 99, 396
PspPI GGNCC 1 cut(s) 333
PstI CTGCAG 2 cut(s) 174, 322
PstNI CAGNNNCTG 1 cut(s) 404
PsyI GACNNNGTC 1 cut(s) 124
PvuI CGATCG 1 cut(s) 153
RruI TCGCGA 1 cut(s) 225
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
SacI GAGCTC 1 cut(s) 78
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 2 cut(s) 33, 150
Sau96I GGNCC 1 cut(s) 333
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 1 cut(s) 78
SfcI CTRYAG 2 cut(s) 170, 318
SinI GGWCC 1 cut(s) 333
SsiI CCGC 1 cut(s) 60
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 5 cut(s) 93, 240, 246, 329, 462
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 2 cut(s) 125, 258
TaqI TCGA 1 cut(s) 153
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TspGWI ACGGA 1 cut(s) 156
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 333
XspI CTAG 1 cut(s) 386
ZraI GACGTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.