FvH4_4g18580

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002365
Physical Location & Seq
Forward (+)
22441850 .. 22444221
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18580.t2

Sequence Viewer

Length: 660 bp
ATGGCAAGCAGGCTTCTACAAGTTGGCACCAAGATCATCGGAGTCGGCAGAAACTACGCCGCCCACGCCAAAGAGCTCGGAAACGCTGTCCCTAAGGAGCCTGTGCTGTTCCTCAAACCGACGTCGTCTTACTTGGAAAACGGAGGCACCATCGAAATCCCTCACCCACTTGAGTCGTTGGACCATGAGGTGGAGCTGGCCGTCGTTATATCCAAGAAAGCTCGGGATGTTCCTCAGGCCAAGGCCATGGAATACGTTGGTGGTTATGCACTTGCTCTTGATATGACTGCCAGAGAAATTCAAGCTACTGCTAAGTCTGCAGGCCTTCCATGGACCATAGCTAAAGGGCAGGACACCTTCACACCAATCAGTTCTGTGCTTTCCCTAGCAACAGTGCCAGACCCTGATGATTTGGAACTTTGGTTGAAGGTGGATGGAGAAGTGAGGCAGAAAGGCTCAACCAAGGATATGATATTTAAAATCCCATTTCTCATCAGCCACATAAGTTCCATTATGACACTTCTCGAAGGAGATGTAATACTAACAGGCACTCCCAAAGGTGTTGGTCCGGTAAAAGCGGGCCAAAAGATAACTGCAGGAATAACAAACCTTGTGGATGTTCAATTCAATGTTGAAAAACGAAGGCAAGGAAGCTCTTGA

Protein Analysis

220

Amino Acids

23.5

Weight (kDa)

6.92

Isoelectric Point (pI)

28.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAA_hydrolase PF01557 11 - 211 2.1e-63 Fumarylacetoacetate (FAA) hydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 125
AccB1I GGYRCC 2 cut(s) 26, 146
AccB7I CCANNNNNTGG 1 cut(s) 190
AciI CCGC 2 cut(s) 60, 578
AcoI YGGCCR 1 cut(s) 198
AcsI RAATTY 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 5 cut(s) 302, 427, 623, 628, 635
AluBI AGCT 6 cut(s) 76, 196, 221, 305, 341, 654
AluI AGCT 6 cut(s) 76, 196, 221, 305, 341, 654
Alw21I GWGCWC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 222
AoxI GGCC 5 cut(s) 198, 237, 243, 322, 580
ApoI RAATTY 1 cut(s) 297
AspS9I GGNCC 4 cut(s) 181, 333, 566, 580
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 222
AvaII GGWCC 3 cut(s) 181, 333, 566
AxyI CCTNAGG 2 cut(s) 93, 234
BanI GGYRCC 2 cut(s) 26, 146
BanII GRGCYC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 2 cut(s) 158, 428
BceAI ACGGC 1 cut(s) 185
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 2 cut(s) 318, 594
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme18I GGWCC 3 cut(s) 181, 333, 566
BmeT110I CYCGRG 1 cut(s) 222
BmgT120I GGNCC 4 cut(s) 181, 333, 566, 580
BmiI GGNNCC 3 cut(s) 28, 99, 148
BpuEI CTTGAG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 4 cut(s) 240, 246, 329, 462
BsaWI WCCGGW 1 cut(s) 568
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse21I CCTNAGG 2 cut(s) 93, 234
BseDI CCNNGG 4 cut(s) 240, 246, 329, 462
BseGI GGATG 3 cut(s) 232, 439, 622
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 248
BshFI GGCC 5 cut(s) 200, 239, 245, 324, 582
BshNI GGYRCC 2 cut(s) 26, 146
BsiHKAI GWGCWC 1 cut(s) 78
BsiHKCI CYCGRG 1 cut(s) 222
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 1 cut(s) 190
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 5 cut(s) 200, 239, 245, 324, 582
BsoBI CYCGRG 1 cut(s) 222
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 33
Bsp19I CCATGG 2 cut(s) 246, 329
BspACI CCGC 2 cut(s) 60, 578
BspANI GGCC 5 cut(s) 200, 239, 245, 324, 582
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 3 cut(s) 28, 99, 148
BspMAI CTGCAG 2 cut(s) 322, 598
BspT107I GGYRCC 2 cut(s) 26, 146
BssECI CCNNGG 4 cut(s) 240, 246, 329, 462
BssMI GATC 1 cut(s) 33
BssNI GRCGYC 1 cut(s) 122
BssT1I CCWWGG 4 cut(s) 240, 246, 329, 462
Bst4CI ACNGT 1 cut(s) 394
BstACI GRCGYC 1 cut(s) 122
BstC8I GCNNGC 5 cut(s) 7, 11, 198, 322, 580
BstDEI CTNAG 3 cut(s) 93, 234, 312
BstDSI CCRYGG 2 cut(s) 246, 329
BstF5I GGATG 3 cut(s) 232, 439, 622
BstKTI GATC 1 cut(s) 36
BstMBI GATC 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 65, 317
BstSFI CTRYAG 2 cut(s) 318, 594
BstXI CCANNNNNNTGG 1 cut(s) 247
Bsu36I CCTNAGG 2 cut(s) 93, 234
BsuRI GGCC 5 cut(s) 200, 239, 245, 324, 582
BtgI CCRYGG 2 cut(s) 246, 329
BtsCI GGATG 3 cut(s) 232, 439, 622
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 5 cut(s) 7, 11, 198, 322, 580
CaiI CAGNNNCTG 1 cut(s) 404
Cfr13I GGNCC 4 cut(s) 181, 333, 566, 580
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 3 cut(s) 185, 247, 330
DdeI CTNAG 3 cut(s) 93, 234, 312
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DraI TTTAAA 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 76
Eco130I CCWWGG 4 cut(s) 240, 246, 329, 462
Eco147I AGGCCT 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 3 cut(s) 181, 333, 566
Eco53kI GAGCTC 1 cut(s) 76
Eco81I CCTNAGG 2 cut(s) 93, 234
Eco88I CYCGRG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 76
EcoT14I CCWWGG 4 cut(s) 240, 246, 329, 462
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 4 cut(s) 240, 246, 329, 462
FaeI CATG 3 cut(s) 188, 250, 333
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 184, 246, 329
FauI CCCGC 1 cut(s) 571
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 3 cut(s) 239, 446, 629
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 60
FspBI CTAG 1 cut(s) 386
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 5 cut(s) 200, 239, 245, 324, 582
HapII CCGG 1 cut(s) 569
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 3 cut(s) 188, 250, 333
HinfI GANTC 2 cut(s) 42, 173
HpaII CCGG 1 cut(s) 569
HphI GGTGA 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 41, 80
Hpy188III TCNNGA 4 cut(s) 224, 278, 524, 657
Hpy99I CGWCG 3 cut(s) 124, 127, 206
HpyAV CCTTC 5 cut(s) 335, 367, 421, 521, 636
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 2 cut(s) 122, 255
HpyCH4V TGCA 3 cut(s) 269, 320, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 317
HpyF3I CTNAG 3 cut(s) 93, 234, 312
HpySE526I ACGT 2 cut(s) 122, 255
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 3 cut(s) 188, 250, 333
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 97, 193
MaeI CTAG 1 cut(s) 386
MaeII ACGT 2 cut(s) 122, 255
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 2 cut(s) 297, 623
MlyI GAGTC 2 cut(s) 51, 182
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 6 cut(s) 122, 137, 171, 181, 243, 438
MseI TTAA 1 cut(s) 477
MspI CCGG 1 cut(s) 569
MwoI GCNNNNNNNGC 2 cut(s) 65, 317
NcoI CCATGG 2 cut(s) 246, 329
NdeII GATC 1 cut(s) 33
NlaIII CATG 3 cut(s) 188, 250, 333
NlaIV GGNNCC 3 cut(s) 28, 99, 148
PceI AGGCCT 1 cut(s) 324
PflFI GACNNNGTC 1 cut(s) 124
PflMI CCANNNNNTGG 1 cut(s) 190
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 2 cut(s) 50, 181
PpsI GAGTC 2 cut(s) 50, 181
Psp124BI GAGCTC 1 cut(s) 78
PspN4I GGNNCC 3 cut(s) 28, 99, 148
PspPI GGNCC 4 cut(s) 181, 333, 566, 580
PstI CTGCAG 2 cut(s) 322, 598
PstNI CAGNNNCTG 1 cut(s) 404
PsyI GACNNNGTC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 78
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 4 cut(s) 181, 333, 566, 580
SchI GAGTC 2 cut(s) 51, 182
SduI GDGCHC 1 cut(s) 78
SfcI CTRYAG 2 cut(s) 318, 594
SinI GGWCC 3 cut(s) 181, 333, 566
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 2 cut(s) 297, 623
SseBI AGGCCT 1 cut(s) 324
SsiI CCGC 2 cut(s) 60, 578
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 78
StuI AGGCCT 1 cut(s) 324
StyI CCWWGG 4 cut(s) 240, 246, 329, 462
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 2 cut(s) 125, 258
TaqI TCGA 2 cut(s) 153, 525
TasI AATT 2 cut(s) 297, 623
TauI GCSGC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 1 cut(s) 399
TspGWI ACGGA 1 cut(s) 156
TspRI CASTG 1 cut(s) 399
Tth111I GACNNNGTC 1 cut(s) 124
Van91I CCANNNNNTGG 1 cut(s) 190
VpaK11BI GGWCC 3 cut(s) 181, 333, 566
XapI RAATTY 1 cut(s) 297
XspI CTAG 1 cut(s) 386
ZraI GACGTC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.