Rorug04G0185000

Acylpyruvase FAHD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002365
Physical Location & Seq
Forward (+)
32181424 .. 32185403
3980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0185000.1

Sequence Viewer

Length: 1029 bp
ATGGCGGGTGTGAGGAGGATGAAGCTCGGGTCACAAGGCCTGGAGGTCTCAGCTCAAGGACTGGGCTGCATGGGCATGTCAGCCTTCTATGGGCCCCCGAAGCCCGAAGCCGACATGATCAAGCTCATCCACCATGCTGTCGACTCCGGCGTCACCTTCCTCGACACCTCCGACATCTACGGTCCCCACACCAACGAAATTCTCCTCGGCAAGGCTTTGAGTGGAGGGGTGAGAGACAAGGTTGAATTGGCCACCAAATTCGGTGCCAGGTCTGCGGACAACAAAAGGGAGATTCGAGGCGAGCCGGCATATGTGAGAGCTGCTTGTGAAGGAAGCTTGAAGCGGCTCGGTGTCGATTCTATTGATCTCTATTATCAGCATCGGATTGACACCCGTGTCCCCACTGAAGTCACGGTGGGGGAGCTGAAGAAGCTAATTGAAGAGGGTAAAATAAAGTACATTGGTCTGTCTGAGGCGTCAGCTTCAACAATAAGAAGAGCCCATGCTGTTCATCCAATAACTGCTGTGCAGCTGGAGTGGTCCTTGTGGTCAAGAGACGTAGAGGAAGAAATAATCCCTACTTGTCGGGAACTTGGCATTGGCATTGTTGCATACAGTCCTCTAGGAAGAGGATTCTTATCATCAGGGGCAAATTTCATCGAAAATCTTCCCAATGATGATTCTCGAAAGAATATGCCTAGGTTCCAAGCTGAAAACCTAGAGCATAATAAAACAATATTTGAGCGAGTGAGTGATCTTGCAGCAAGGAAGGGTTGCACTCCATCTCAACTAGCTCTGGCCTGGGTTCATCACCAAGGAAATGATGTGTGCCCTATACCCGGAACCACCAAGATTGAGAATTTTAACCAGAACATTAAGGCTCTGTCTGTGAAACTGACACCAGAAGAAATGGCTGAGCTTGAATCTTTTGCTTCTGCAGATGCTGTTAAGGGCGACAGATATCAGACTGGCCTCAGTACTTGGAGGAACTCTGACACTCCACCACTGTCTTCATGGAAAGCCACATAA

Protein Analysis

342

Amino Acids

37.5

Weight (kDa)

6.52

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 23 - 310 3.2e-72 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 149, 395
AccB1I GGYRCC 1 cut(s) 263
AccI GTMKAC 1 cut(s) 141
AciI CCGC 3 cut(s) 5, 275, 343
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 4 cut(s) 198, 257, 652, 859
AcuI CTGAAG 2 cut(s) 426, 446
AcyI GRCGYC 2 cut(s) 150, 476
AfaI GTAC 2 cut(s) 458, 979
AfiI CCNNNNNNNGG 3 cut(s) 90, 211, 839
AgsI TTSAA 5 cut(s) 245, 340, 440, 486, 923
AjnI CCWGG 3 cut(s) 39, 266, 800
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
Alw26I GTCTC 3 cut(s) 52, 228, 549
AlwNI CAGNNNCTG 1 cut(s) 944
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 5 cut(s) 37, 92, 249, 798, 970
ApaI GGGCCC 1 cut(s) 96
ApeKI GCWGC 4 cut(s) 66, 320, 529, 761
ApoI RAATTY 4 cut(s) 198, 257, 652, 859
Asp700I GAANNNNTTC 1 cut(s) 666
AspA2I CCTAGG 1 cut(s) 698
AspS9I GGNCC 4 cut(s) 92, 93, 182, 540
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 3 cut(s) 145, 241, 803
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 2 cut(s) 182, 540
AvrII CCTAGG 1 cut(s) 698
BaeGI GKGCMC 2 cut(s) 96, 833
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 2 cut(s) 96, 502
BbsI GAAGAC 1 cut(s) 1002
BbvI GCAGC 4 cut(s) 53, 307, 541, 773
BccI CCATC 1 cut(s) 790
BciT130I CCWGG 3 cut(s) 41, 268, 802
BclI TGATCA 1 cut(s) 117
BcnI CCSGG 1 cut(s) 840
BcoDI GTCTC 3 cut(s) 52, 228, 549
BfaI CTAG 4 cut(s) 623, 699, 719, 791
BfmI CTRYAG 1 cut(s) 936
BisI GCNGC 5 cut(s) 67, 321, 344, 530, 762
BlnI CCTAGG 1 cut(s) 698
BlpI GCTNAGC 1 cut(s) 915
BlsI GCNGC 5 cut(s) 68, 322, 345, 531, 763
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 4 cut(s) 41, 268, 802, 840
Bme18I GGWCC 2 cut(s) 182, 540
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 4 cut(s) 92, 93, 182, 540
BmiI GGNNCC 6 cut(s) 94, 95, 184, 265, 704, 844
BmrFI CCNGG 4 cut(s) 41, 268, 802, 840
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 2 cut(s) 388, 931
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 1002
BpmI CTGGAG 2 cut(s) 62, 554
Bpu1102I GCTNAGC 1 cut(s) 915
BpuEI CTTGAG 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 840
BsaBI GATNNNNATC 1 cut(s) 637
BsaHI GRCGYC 2 cut(s) 150, 476
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 4 cut(s) 205, 698, 801, 814
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 211, 839
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 66, 973
Bse8I GATNNNNATC 1 cut(s) 637
BseBI CCWGG 3 cut(s) 41, 268, 802
BseDI CCNNGG 4 cut(s) 205, 698, 801, 814
BseGI GGATG 3 cut(s) 24, 126, 511
BseJI GATNNNNATC 1 cut(s) 637
BseLI CCNNNNNNNGG 3 cut(s) 90, 211, 839
BseMII CTCAG 4 cut(s) 63, 462, 906, 988
BseNI ACTGG 2 cut(s) 66, 973
BseRI GAGGAG 2 cut(s) 28, 194
BseSI GKGCMC 2 cut(s) 96, 833
BseXI GCAGC 4 cut(s) 53, 307, 541, 773
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 5 cut(s) 39, 94, 251, 800, 972
BshNI GGYRCC 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 147, 305, 840
BslFI GGGAC 2 cut(s) 168, 383
BslI CCNNNNNNNGG 3 cut(s) 90, 211, 839
BsmAI GTCTC 3 cut(s) 52, 228, 549
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 2 cut(s) 168, 383
BsnI GGCC 5 cut(s) 39, 94, 251, 800, 972
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 26
Bsp120I GGGCCC 1 cut(s) 92
Bsp1286I GDGCHC 3 cut(s) 96, 502, 833
Bsp143I GATC 3 cut(s) 117, 364, 754
Bsp1720I GCTNAGC 1 cut(s) 915
BspACI CCGC 3 cut(s) 5, 275, 343
BspANI GGCC 5 cut(s) 39, 94, 251, 800, 972
BspCNI CTCAG 4 cut(s) 62, 463, 907, 987
BspLI GGNNCC 6 cut(s) 94, 95, 184, 265, 704, 844
BspMAI CTGCAG 1 cut(s) 940
BspQI GCTCTTC 1 cut(s) 490
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 66, 973
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 4 cut(s) 205, 698, 801, 814
BssMI GATC 3 cut(s) 117, 364, 754
BssNI GRCGYC 2 cut(s) 150, 476
BssT1I CCWWGG 2 cut(s) 698, 814
Bst2UI CCWGG 3 cut(s) 41, 268, 802
Bst4CI ACNGT 4 cut(s) 182, 415, 617, 1008
Bst6I CTCTTC 3 cut(s) 435, 490, 622
BstACI GRCGYC 2 cut(s) 150, 476
BstC8I GCNNGC 2 cut(s) 302, 306
BstDEI CTNAG 4 cut(s) 49, 471, 915, 974
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 24, 126, 511
BstKTI GATC 3 cut(s) 120, 367, 757
BstMAI GTCTC 3 cut(s) 52, 228, 549
BstMBI GATC 3 cut(s) 117, 364, 754
BstMWI GCNNNNNNNGC 4 cut(s) 72, 100, 272, 430
BstNI CCWGG 3 cut(s) 41, 268, 802
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 4 cut(s) 39, 266, 800, 838
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 2 cut(s) 96, 833
BstV1I GCAGC 4 cut(s) 53, 307, 541, 773
BstV2I GAAGAC 1 cut(s) 1002
BsuRI GGCC 5 cut(s) 39, 94, 251, 800, 972
BtsCI GGATG 3 cut(s) 24, 126, 511
BtsIMutI CAGTG 2 cut(s) 402, 1004
Cac8I GCNNGC 2 cut(s) 302, 306
CaiI CAGNNNCTG 1 cut(s) 944
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 4 cut(s) 92, 93, 182, 540
CseI GACGC 2 cut(s) 139, 465
Csp6I GTAC 2 cut(s) 457, 978
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 6 cut(s) 70, 76, 115, 134, 503, 1014
CviQI GTAC 2 cut(s) 457, 978
DdeI CTNAG 4 cut(s) 49, 471, 915, 974
DpnI GATC 3 cut(s) 119, 366, 756
DpnII GATC 3 cut(s) 117, 364, 754
DrdI GACNNNNNNGTC 2 cut(s) 149, 395
DseDI GACNNNNNNGTC 2 cut(s) 149, 395
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 3 cut(s) 435, 490, 622
EarI CTCTTC 3 cut(s) 435, 490, 622
Eco130I CCWWGG 2 cut(s) 698, 814
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 2 cut(s) 96, 502
Eco31I GGTCTC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 962
Eco47I GGWCC 2 cut(s) 182, 540
Eco57I CTGAAG 2 cut(s) 426, 446
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 93
EcoRII CCWGG 3 cut(s) 39, 266, 800
EcoRV GATATC 1 cut(s) 962
EcoT14I CCWWGG 2 cut(s) 698, 814
EcoT38I GRGCYC 2 cut(s) 96, 502
ErhI CCWWGG 2 cut(s) 698, 814
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
FaqI GGGAC 2 cut(s) 168, 383
FatI CATG 6 cut(s) 69, 75, 114, 133, 502, 1013
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 117
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 5 cut(s) 67, 321, 344, 530, 762
FokI GGATG 3 cut(s) 31, 113, 498
FriOI GRGCYC 2 cut(s) 96, 502
Fsp4HI GCNGC 5 cut(s) 67, 321, 344, 530, 762
FspBI CTAG 4 cut(s) 623, 699, 719, 791
GluI GCNGC 5 cut(s) 67, 321, 344, 530, 762
GsuI CTGGAG 2 cut(s) 62, 554
HaeIII GGCC 5 cut(s) 39, 94, 251, 800, 972
HapII CCGG 3 cut(s) 147, 305, 840
HgaI GACGC 2 cut(s) 139, 465
Hin1I GRCGYC 2 cut(s) 150, 476
Hin1II CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 6 cut(s) 143, 292, 356, 633, 680, 923
HpaII CCGG 3 cut(s) 147, 305, 840
HphI GGTGA 3 cut(s) 145, 241, 803
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 5 cut(s) 172, 384, 472, 966, 994
Hpy188III TCNNGA 3 cut(s) 552, 587, 684
Hpy8I GTNNAC 1 cut(s) 142
HpyAV CCTTC 4 cut(s) 94, 166, 323, 763
HpyCH4III ACNGT 4 cut(s) 182, 415, 617, 1008
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 6 cut(s) 69, 529, 611, 761, 777, 938
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 100, 272, 430
HpyF3I CTNAG 4 cut(s) 49, 471, 915, 974
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 2 cut(s) 150, 476
Hsp92II CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 3 cut(s) 117, 364, 754
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 4 cut(s) 53, 307, 541, 773
LweI GCATC 2 cut(s) 388, 931
MaeI CTAG 4 cut(s) 623, 699, 719, 791
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 3 cut(s) 30, 151, 409
MalI GATC 3 cut(s) 119, 366, 756
MboI GATC 3 cut(s) 117, 364, 754
MboII GAAGA 8 cut(s) 439, 452, 507, 578, 639, 659, 917, 1002
MhlI GDGCHC 3 cut(s) 96, 502, 833
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 6 cut(s) 198, 245, 257, 435, 652, 859
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 251
MroNI GCCGGC 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 666
MscI TGGCCA 1 cut(s) 251
MseI TTAA 3 cut(s) 864, 876, 948
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 532
MspI CCGG 3 cut(s) 147, 305, 840
MspR9I CCNGG 4 cut(s) 41, 268, 802, 840
MvaI CCWGG 3 cut(s) 41, 268, 802
MwoI GCNNNNNNNGC 4 cut(s) 72, 100, 272, 430
NaeI GCCGGC 1 cut(s) 306
NciI CCSGG 1 cut(s) 840
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 117, 364, 754
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 6 cut(s) 73, 79, 118, 137, 506, 1017
NlaIV GGNNCC 6 cut(s) 94, 95, 184, 265, 704, 844
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 3 cut(s) 30, 151, 409
NspI RCATGY 1 cut(s) 79
PceI AGGCCT 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 168
PdiI GCCGGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 5 cut(s) 292, 356, 633, 680, 923
PkrI GCNGC 5 cut(s) 68, 322, 345, 531, 763
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp6I CCWGG 3 cut(s) 39, 266, 800
PspGI CCWGG 3 cut(s) 39, 266, 800
PspN4I GGNNCC 6 cut(s) 94, 95, 184, 265, 704, 844
PspOMI GGGCCC 1 cut(s) 92
PspPI GGNCC 4 cut(s) 92, 93, 182, 540
PstI CTGCAG 1 cut(s) 940
PstNI CAGNNNCTG 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 2 cut(s) 458, 979
RsaNI GTAC 2 cut(s) 457, 978
RseI CAYNNNNRTG 1 cut(s) 74
SalI GTCGAC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 3 cut(s) 864, 876, 948
SatI GCNGC 5 cut(s) 67, 321, 344, 530, 762
Sau3AI GATC 3 cut(s) 117, 364, 754
Sau96I GGNCC 4 cut(s) 92, 93, 182, 540
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 4 cut(s) 41, 268, 802, 840
SduI GDGCHC 3 cut(s) 96, 502, 833
SfaNI GCATC 2 cut(s) 388, 931
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 2 cut(s) 182, 540
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 6 cut(s) 198, 245, 257, 435, 652, 859
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 3 cut(s) 5, 275, 343
SspI AATATT 1 cut(s) 738
SspMI CTAG 4 cut(s) 623, 699, 719, 791
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 4 cut(s) 39, 266, 800, 838
StyI CCWWGG 2 cut(s) 698, 814
TaaI ACNGT 4 cut(s) 182, 415, 617, 1008
TaiI ACGT 1 cut(s) 561
TaqI TCGA 6 cut(s) 141, 162, 295, 354, 660, 685
TasI AATT 6 cut(s) 198, 245, 257, 435, 652, 859
TatI WGTACW 2 cut(s) 456, 977
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 5 cut(s) 292, 356, 633, 680, 923
Tru1I TTAA 3 cut(s) 864, 876, 948
Tru9I TTAA 3 cut(s) 864, 876, 948
TscAI CASTG 2 cut(s) 409, 1011
TseFI GTSAC 3 cut(s) 30, 151, 409
TseI GCWGC 4 cut(s) 66, 320, 529, 761
Tsp45I GTSAC 3 cut(s) 30, 151, 409
TspDTI ATGAA 5 cut(s) 35, 500, 646, 797, 1002
TspRI CASTG 2 cut(s) 409, 1011
VpaK11BI GGWCC 2 cut(s) 182, 540
XagI CCTNNNNNAGG 1 cut(s) 209
XapI RAATTY 4 cut(s) 198, 257, 652, 859
XceI RCATGY 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 1011
XmaJI CCTAGG 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 4 cut(s) 623, 699, 719, 791
ZrmI AGTACT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.