AT3G27860
ERF Family

Tudor PWWP MBT superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002210
Physical Location & Seq
Forward (+)
10325834 .. 10328323
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27860.1

Sequence Viewer

Length: 1959 bp
ATGGGGAATATTCTACCGACTGGGTCTAGGGTTTCTGAGAACGAGGGTTCTGATGACAACAAATTGAAGGAGCACGATGTTAAGATGGAGAATGTTAAGGAAGACTCTGAACAAATTGGAGATAATGTGAGGAGTGGGGTTTCTTCATTGAGAGATAATTTTGAAGAATTAGAAAATGGGTTTCATGTGGGAGATTTTGTTTGGGGAGAAGAAGCCAATAGTCAACAATGGTGGCCAGGTCAGATTTATGATTCTTTAGATGCTTCAGACTTGGCTTTGAAGACAATGCAGAAAGGTAAATTACTTGTGGCGTATTTTGGAGATGGGAGCTTTTTTGGTTGGTGTAATCCATTGGAATTGAAACCGTTTCTTGAGAATTTCAAAGAGTTTTCGAAGATGAGTGATTCGAGAAGGTTTCTTCTTGCTGTGGAAGATGCTGTGAGAGAGATTGGTGAGCATGTTGAGAAGTTTTTGGTTTGTGATGACGCTGCTTTAGTTAGTTCTGTAGCTTTGAATTTGGGGATTAAGGATGGTGTTGTTGTGCCTGATGTTAGAAGAAAGATTATATCGTCTTTGGTTCTTGAAAACCCCGGTGTTGTTCTTGAAGATGTTAAAAGACTTGCCATGACGGTTAGATTTGATGATTTGTTAGAGATTGAGGTTTTGAGGAGGAAGATATCGGCGTTTTATCGGTGTAAAGGAAGGTTTGATTTAGCTAAGTTTGATGAACATCGATATATTATAGGACTTGAAGACAAAGAGCATGAATCTTGTCAGAGATTATTGAGAAAGTGTTCAGGTTTTGCTAGCAAAAAGAGGAAATGTGGTGATGTAGCAACCACTGGTTCTACTACTTTGAGGAAGAAAAGACTGAGTGAAGTTTCGAAGATTGAAACCGCTGAGAAAGAAATTAGTAACGGGAAGAGTTTGTCGTCGAGGAAGAGAAAGAGCAAAAGGGGTTTGGATGAGAATGATGATGATGGGATTGAGAAAAGAGAAGAGTCGAACGACTCAAACCATTTAGAAGAGAGTGAGAAGAAAGATGATTTAGCAACTCCATTGGCTTCAATCTGCAAAAGGCTCAATGTTGATGTTTCTTCATGTGTTAAAAGGTGTAATGGAAATGGCGAGGCAATATTGCAGACAGGTAAAAGAGAGAGGAAGAAAAGCAAGTATCTTTCTCCTGAATACATGACAGATTTTAGTTGCAGAGCGAGAAAGATCAAAATAGAATCTGCTGAATCAAGTCAGATCCAAGTAGCAGTGCGAATGACAACGCCTAATACAGCCATCGATGTTGTGAAACTGGGAGCTACACCAGAGGAAATGCTGGCTCTAATCCGTGCGGCTGCTCTCAATGCACAATATCCAAAAGACTATAATAGTACCTCATGTGACATGGTAAGAGAGTTTGTGTCCAATTATCGAAGCTTCAATAACAAGAGGAACCTTTCAGATGTAGAGAAGCAGCCTGAAGTGAAACAGGAAATAGTGGATGAGAAAGAGAAAACCAGAAATGAACCAGGTGTTGAGCTATATATCAAGACTGGTTTTGGTTCTACTCTGCCTTCAAAAGATGATTTGATCAAAACATACGAAAAGTTTGGAGCTTTAGACAAAGAGAGAAGCTATATGTTCAACAATAACTCATGCTCATGCGTTGCCTTTGTGAATGCATCTGATGGAGAAGAAGCTTTTAACAGGTCATTAGAGAAATGTCCATTTGCCACTACTTCCACAGTAACATTCAAGCTCGAGTATCCATCTTCTGCTTCATCTGAGAAGAAGGAAGCTGAAACAAGAAAGGGCGTAACAGAAATCGAGTGTTTGAAAGAGAAGCTCGAGGGGATAAGAGCATTGTTAGATCAATCAGAGGGAAAGATTACGGAAGAATTGAAAATGAAACTTGAAGATGAATCAAGAAACTTGCTTGACAAGGTAAGGAAGATGATTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

652

Amino Acids

73.67

Weight (kDa)

5.75

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 64 - 150 6.9e-11 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 897, 1346
AclWI GGATC 1 cut(s) 1246
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 2 cut(s) 376, 514
AcuI CTGAAG 2 cut(s) 249, 1494
AfaI GTAC 1 cut(s) 1387
AjnI CCWGG 2 cut(s) 235, 1522
Alw21I GWGCWC 1 cut(s) 75
AlwI GGATC 1 cut(s) 1246
Ama87I CYCGRG 2 cut(s) 1754, 1841
AoxI GGCC 1 cut(s) 233
ApeKI GCWGC 3 cut(s) 488, 1349, 1468
ApoI RAATTY 2 cut(s) 376, 514
Asp700I GAANNNNTTC 1 cut(s) 793
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 2 cut(s) 464, 839
AsuII TTCGAA 2 cut(s) 392, 884
AsuNHI GCTAGC 1 cut(s) 806
AvaI CYCGRG 2 cut(s) 1754, 1841
BalI TGGCCA 1 cut(s) 235
BbsI GAAGAC 3 cut(s) 108, 287, 759
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 3 cut(s) 475, 1336, 1480
BccI CCATC 7 cut(s) 79, 317, 524, 974, 1298, 1676, 1771
BciT130I CCWGG 2 cut(s) 237, 1524
BciVI GTATCC 1 cut(s) 1770
BclI TGATCA 1 cut(s) 1584
BcnI CCSGG 1 cut(s) 591
BfaI CTAG 2 cut(s) 27, 807
BfmI CTRYAG 1 cut(s) 504
BfuI GTATCC 1 cut(s) 1770
BisI GCNGC 4 cut(s) 489, 1347, 1350, 1469
BlsI GCNGC 4 cut(s) 490, 1348, 1351, 1470
Bme1390I CCNGG 3 cut(s) 237, 591, 1524
BmeT110I CYCGRG 2 cut(s) 1754, 1841
BmiI GGNNCC 1 cut(s) 1448
BmrFI CCNGG 3 cut(s) 237, 591, 1524
BmrI ACTGGG 2 cut(s) 30, 1316
BmsI GCATC 3 cut(s) 250, 424, 1685
BmtI GCTAGC 1 cut(s) 810
BmuI ACTGGG 2 cut(s) 30, 1316
BpiI GAAGAC 3 cut(s) 108, 287, 759
Bpu14I TTCGAA 2 cut(s) 392, 884
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 591
Bsa29I ATCGAT 2 cut(s) 733, 1293
BsaBI GATNNNNATC 1 cut(s) 729
BsaJI CCNNGG 1 cut(s) 589
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 183, 213
Bse1I ACTGG 4 cut(s) 25, 847, 1311, 1552
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 2 cut(s) 237, 1524
BseCI ATCGAT 2 cut(s) 733, 1293
BseDI CCNNGG 1 cut(s) 589
BseGI GGATG 3 cut(s) 535, 970, 1501
BseJI GATNNNNATC 1 cut(s) 729
BseMII CTCAG 4 cut(s) 27, 863, 891, 1770
BseNI ACTGG 4 cut(s) 25, 847, 1311, 1552
BseRI GAGGAG 2 cut(s) 145, 682
BseXI GCAGC 3 cut(s) 475, 1336, 1480
BshFI GGCC 1 cut(s) 235
BshVI ATCGAT 2 cut(s) 733, 1293
BsiHKAI GWGCWC 1 cut(s) 75
BsiHKCI CYCGRG 2 cut(s) 1754, 1841
BsiSI CCGG 1 cut(s) 591
BsmI GAATGC 1 cut(s) 1678
BsnI GGCC 1 cut(s) 235
BsoBI CYCGRG 2 cut(s) 1754, 1841
Bsp119I TTCGAA 2 cut(s) 392, 884
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 4 cut(s) 1221, 1251, 1584, 1864
BspACI CCGC 2 cut(s) 897, 1346
BspANI GGCC 1 cut(s) 235
BspCNI CTCAG 4 cut(s) 28, 864, 892, 1771
BspDI ATCGAT 2 cut(s) 733, 1293
BspLI GGNNCC 1 cut(s) 1448
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 1 cut(s) 1246
BspT104I TTCGAA 2 cut(s) 392, 884
BsrI ACTGG 4 cut(s) 25, 847, 1311, 1552
BssECI CCNNGG 1 cut(s) 589
BssMI GATC 4 cut(s) 1221, 1251, 1584, 1864
Bst2UI CCWGG 2 cut(s) 237, 1524
Bst4CI ACNGT 3 cut(s) 366, 631, 1741
Bst6I CTCTTC 4 cut(s) 917, 935, 993, 1020
BstBI TTCGAA 2 cut(s) 392, 884
BstC8I GCNNGC 2 cut(s) 808, 1332
BstDEI CTNAG 5 cut(s) 36, 717, 872, 900, 1779
BstF5I GGATG 3 cut(s) 535, 970, 1501
BstKTI GATC 4 cut(s) 1224, 1254, 1587, 1867
BstMBI GATC 4 cut(s) 1221, 1251, 1584, 1864
BstMWI GCNNNNNNNGC 1 cut(s) 1358
BstNI CCWGG 2 cut(s) 237, 1524
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 3 cut(s) 235, 589, 1522
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 3 cut(s) 475, 1336, 1480
BstV2I GAAGAC 3 cut(s) 108, 287, 759
BstX2I RGATCY 1 cut(s) 1251
BstYI RGATCY 1 cut(s) 1251
Bsu15I ATCGAT 2 cut(s) 733, 1293
BsuI GTATCC 1 cut(s) 1770
BsuRI GGCC 1 cut(s) 235
BsuTUI ATCGAT 2 cut(s) 733, 1293
BtsCI GGATG 3 cut(s) 535, 970, 1501
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 2 cut(s) 840, 1269
Cac8I GCNNGC 2 cut(s) 808, 1332
ClaI ATCGAT 2 cut(s) 733, 1293
CseI GACGC 1 cut(s) 494
CsiI ACCWGGT 1 cut(s) 1522
Csp6I GTAC 1 cut(s) 1386
CspCI CAANNNNNGTGG 2 cut(s) 212, 247
CviQI GTAC 1 cut(s) 1386
DdeI CTNAG 5 cut(s) 36, 717, 872, 900, 1779
DpnI GATC 4 cut(s) 1223, 1253, 1586, 1866
DpnII GATC 4 cut(s) 1221, 1251, 1584, 1864
EaeI YGGCCR 1 cut(s) 233
Eam1104I CTCTTC 4 cut(s) 917, 935, 993, 1020
EarI CTCTTC 4 cut(s) 917, 935, 993, 1020
Eco32I GATATC 1 cut(s) 678
Eco57I CTGAAG 2 cut(s) 249, 1494
Eco88I CYCGRG 2 cut(s) 1754, 1841
EcoRII CCWGG 2 cut(s) 235, 1522
EcoRV GATATC 1 cut(s) 678
EcoT22I ATGCAT 1 cut(s) 1678
FbaI TGATCA 1 cut(s) 1584
Fnu4HI GCNGC 4 cut(s) 489, 1347, 1350, 1469
FokI GGATG 3 cut(s) 542, 977, 1508
Fsp4HI GCNGC 4 cut(s) 489, 1347, 1350, 1469
FspBI CTAG 2 cut(s) 27, 807
GluI GCNGC 4 cut(s) 489, 1347, 1350, 1469
HaeIII GGCC 1 cut(s) 235
HapII CCGG 1 cut(s) 591
HgaI GACGC 1 cut(s) 494
HincII GTYRAC 1 cut(s) 224
HindII GTYRAC 1 cut(s) 224
HindIII AAGCTT 2 cut(s) 1429, 1692
HinfI GANTC 9 cut(s) 104, 251, 404, 767, 1001, 1010, 1232, 1241, 1916
HpaII CCGG 1 cut(s) 591
HphI GGTGA 2 cut(s) 464, 839
Hpy166II GTNNAC 1 cut(s) 224
Hpy188III TCNNGA 7 cut(s) 371, 408, 581, 602, 1184, 1543, 1920
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 1 cut(s) 937
HpyAV CCTTC 5 cut(s) 61, 405, 696, 1578, 1780
HpyCH4III ACNGT 3 cut(s) 366, 631, 1741
HpyCH4V TGCA 6 cut(s) 289, 1074, 1141, 1209, 1361, 1676
HpyF10VI GCNNNNNNNGC 1 cut(s) 1358
HpyF3I CTNAG 5 cut(s) 36, 717, 872, 900, 1779
Ksp22I TGATCA 1 cut(s) 1584
Kzo9I GATC 4 cut(s) 1221, 1251, 1584, 1864
LmnI GCTCC 4 cut(s) 70, 327, 1310, 1607
Lsp1109I GCAGC 3 cut(s) 475, 1336, 1480
LweI GCATC 3 cut(s) 250, 424, 1685
MabI ACCWGGT 1 cut(s) 1522
MaeI CTAG 2 cut(s) 27, 807
MaeIII GTNAC 4 cut(s) 914, 1394, 1741, 1810
MalI GATC 4 cut(s) 1223, 1253, 1586, 1866
MboI GATC 4 cut(s) 1221, 1251, 1584, 1864
MflI RGATCY 1 cut(s) 1251
MhlI GDGCHC 1 cut(s) 75
MlsI TGGCCA 1 cut(s) 235
MluNI TGGCCA 1 cut(s) 235
MlyI GAGTC 3 cut(s) 98, 1004, 1010
Mox20I TGGCCA 1 cut(s) 235
Mph1103I ATGCAT 1 cut(s) 1678
MroXI GAANNNNTTC 1 cut(s) 793
MscI TGGCCA 1 cut(s) 235
MseI TTAA 6 cut(s) 81, 96, 525, 612, 1107, 1698
MslI CAYNNNNRTG 1 cut(s) 1654
Msp20I TGGCCA 1 cut(s) 235
MspA1I CMGCKG 1 cut(s) 899
MspI CCGG 1 cut(s) 591
MspR9I CCNGG 3 cut(s) 237, 591, 1524
Mva1269I GAATGC 1 cut(s) 1678
MvaI CCWGG 2 cut(s) 237, 1524
MwoI GCNNNNNNNGC 1 cut(s) 1358
NciI CCSGG 1 cut(s) 591
NdeII GATC 4 cut(s) 1221, 1251, 1584, 1864
NheI GCTAGC 1 cut(s) 806
NlaIV GGNNCC 1 cut(s) 1448
NmuCI GTSAC 1 cut(s) 1394
NsiI ATGCAT 1 cut(s) 1678
NspI RCATGY 1 cut(s) 461
NspV TTCGAA 2 cut(s) 392, 884
PaeR7I CTCGAG 2 cut(s) 1754, 1841
PctI GAATGC 1 cut(s) 1678
PdmI GAANNNNTTC 1 cut(s) 793
PfeI GAWTC 6 cut(s) 251, 404, 767, 1232, 1241, 1916
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 4 cut(s) 490, 1348, 1351, 1470
PleI GAGTC 3 cut(s) 98, 1004, 1009
PpsI GAGTC 3 cut(s) 98, 1004, 1009
Psp6I CCWGG 2 cut(s) 235, 1522
PspGI CCWGG 2 cut(s) 235, 1522
PspN4I GGNNCC 1 cut(s) 1448
PspXI VCTCGAGB 2 cut(s) 1754, 1841
PsuI RGATCY 1 cut(s) 1251
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 1 cut(s) 1387
RsaNI GTAC 1 cut(s) 1386
RseI CAYNNNNRTG 1 cut(s) 1654
SaqAI TTAA 6 cut(s) 81, 96, 525, 612, 1107, 1698
SatI GCNGC 4 cut(s) 489, 1347, 1350, 1469
Sau3AI GATC 4 cut(s) 1221, 1251, 1584, 1864
SchI GAGTC 3 cut(s) 98, 1004, 1010
ScrFI CCNGG 3 cut(s) 237, 591, 1524
SduI GDGCHC 1 cut(s) 75
SexAI ACCWGGT 1 cut(s) 1522
SfaNI GCATC 3 cut(s) 250, 424, 1685
SfcI CTRYAG 1 cut(s) 504
Sfr274I CTCGAG 2 cut(s) 1754, 1841
SfuI TTCGAA 2 cut(s) 392, 884
SlaI CTCGAG 2 cut(s) 1754, 1841
SmiMI CAYNNNNRTG 1 cut(s) 1654
SmlI CTYRAG 3 cut(s) 371, 1754, 1841
SmoI CTYRAG 3 cut(s) 371, 1754, 1841
SsiI CCGC 2 cut(s) 897, 1346
SspI AATATT 2 cut(s) 10, 1137
SspMI CTAG 2 cut(s) 27, 807
StyD4I CCNGG 3 cut(s) 235, 589, 1522
TaaI ACNGT 3 cut(s) 366, 631, 1741
TauI GCSGC 1 cut(s) 1349
TfiI GAWTC 6 cut(s) 251, 404, 767, 1232, 1241, 1916
Tru1I TTAA 6 cut(s) 81, 96, 525, 612, 1107, 1698
Tru9I TTAA 6 cut(s) 81, 96, 525, 612, 1107, 1698
TscAI CASTG 2 cut(s) 847, 1269
TseFI GTSAC 1 cut(s) 1394
TseI GCWGC 3 cut(s) 488, 1349, 1468
Tsp45I GTSAC 1 cut(s) 1394
TspDTI ATGAA 9 cut(s) 135, 173, 741, 780, 1089, 1533, 1764, 1916, 1929
TspGWI ACGGA 2 cut(s) 1331, 1901
TspRI CASTG 2 cut(s) 847, 1269
Tth111I GACNNNGTC 1 cut(s) 22
XapI RAATTY 2 cut(s) 376, 514
XceI RCATGY 1 cut(s) 461
XhoI CTCGAG 2 cut(s) 1754, 1841
XmnI GAANNNNTTC 1 cut(s) 793
XspI CTAG 2 cut(s) 27, 807
Zsp2I ATGCAT 1 cut(s) 1678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.