FvH4_6g50890

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002210
Physical Location & Seq
Reverse (-)
37807007 .. 37813022
6016 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50890.t1

Sequence Viewer

Length: 3327 bp
ATGGGGACGTTGGGGAGATCTACAGCCCTAGGTAGTGGTTCGGGTGGTTCCGGGTCGGTTTCGGGTTCCGGAGCGGCTAGGTTTGAGGCCGCGGAGGAGAAGGCGAAGGTTGATGACTCCGGCGGGAGGGGAGGGTCCGGCGGAGAGCTGGTTAGGTCGTTTGTGGAGCTGGTTAAGACTGTGGAGGCGAAAGTCTCGGCGGGGTTGAGTTTCGGGGAGCTGGATACGTGTATGTTGAGAGGGAGTGAGAGTGTTGGAGAGATGGAGTCTGGTTTGGGGGATGAGAGGAGAGTGGTGAGTATTGGTTCGAAGACGAATTCGGATGCGAATGTTAATGGGAATTGTTGCAGCGAAACCAAGGCTAGTTCTGGTGTGGACGGCGGTGTTGAGGAGAATGGGAATTCTTGTGATGTTACCAAGGCTGATTCTGGTGTGAACGATGGGGTTGAGGATAATGGGAATTCTTGTGATGTTACTAAGGCCGATTCTGGTGTGAACAACGGGGTTGAGGAGAATGGGAAGTCTTTGGATGAAATTGGAGAAACTGAGGTGATTGAGGTTCCCGTTGCAGGGACGAGTGAGAGTGGTGAAGACCGGGAAGGAGAAGAGGTGGGTGACGGAGTATATGAGTTCTGTATTGGAGATTTTGTGTGGGGTAAGATTAAGAACCACCCGTGGTGGCCGGGCCAGATTTGTGATCCTTCAGATGCTTCTGAGCATGCAGCGAAATTGAAATCCAAGGACAAACTTCTTGTTGCTTACTTTGGGGATGACACTTTTGCCTGGTGCCATCCGTCGCAGTTGAAACCCTTTGAGGAATATTTCGTGGAGAAGTCTAAGCTGAGTAGCTCCAGTGTTTTTGTTATTGCTGTGCAGCAGGCTGTGGATGAGATTGGTAGGCTTGTGAAGTTGAAGATGAGTTGTGCTTGCATGGGGGAAGAGTTTATAAGCAGTTGTAACCGACCCTTAGCATCGAATGCTGGAGTTAAGGAAGGAGTTGTTGTCCCTGAAGGCCGAGTTGGGAAGTTTTTTGGTCATCTATCTCAGCCGGTTGACCTTCTTGCTGAATTGAAACATATTGCACAAGTTGCTTCCTTGACTAGTGCTCTTGAGCTCAATGTATTAAAGAGTCTGCTGTCTTCCTTCTATTTTTCGAAAGGGGGCTATCAGTTACCTGTTTACAGTGAACCGCAGCTCATTCTTGGTCTTGAAGATGAGCGGAAGATGGTGGAAGTCCTAGTGCAGTGGTCATCTGATGACATGCCGTCTTCTCCCATAGGCTCCAATACTGGAAATGCTGAACAAGTTTTGTTGCAAAGGTCACCACCAAGTATAGAAAATAGGCAGCCCCAGAAAAGGAAGGAGAAGAGCATTGCTGATCTTCTTGGAGCAGCCAATGGTGTTGAGGGTAAAAGCATGGATGTGGTTGTAGCTGAAGAAGGTACAAGCTCAGAGAAACGAGGGGTATCATCCAGTCAGAAGAAGCAGAAAAGTAGTGATATTCATGGTGAGGGTATATTGATTCTCAAATCAGGTGAGGAAAGAGGCAAATTCTCAGAGTCTCCCATAAGCACTAAGAAGGCTCCATTGGCAAGGAGAAGGAAAAAAGATGAAGGTATTGGATTCAACAGTAGTGATGGTTTGGCTAAAGAAAGTGCTGGTGAAGAGGAAGAAAGTAAGAAGACTCCACTGTCAAGGAGAAGGAAGAGGGATGAATGTGTTAGCACTGACGGTAAAGATAGTGATGCCCTAGAAAAAGCTGGTGGAACGGATGAAACTAAGAAGGCTCCCTTGTCAAGGAGAAGGAAGAAGAATGAAGATACTCACATCAACAGTAATGATGGTGAGGCCAATGTTGGTCTGCAAACAGACATGAAGGACCAAATTGATAAGGGCTCTTTGTCGAGGGAAAGAAAAAAAAGCAAGTACTTGTCACCACCCTTCACAATAAGTGACACAAGGAAAAGAAAACCAAACCTGGAAACAGAATCTCCAAATGCAGTCACGAGTCATGGTTTGGGGGCAGAGGGGGAAGTGCGAGATATGGAATTAGAAGTATCTCTGGAGGCTTCTAATAAACCTCAGTTTGGGGAGAGGATGACCAGGGCTGCTGCCAACCTTAAGGGGTCCCCTCAGGTTTTGAATCGTTGCAGTGAGGAATTACAAGAGGAACAATCGATAGATGGTGAGAATAACAGCACCGAAGTTAAAACACCAACAGAAGATGAGAAGAAGCGTGTTGATCCAATGAGAGCTAATGCAGCAACTAATGAAGTGCTATCTGGAGTCCTCTCTGCTGCTGTTGATCCATTATATCCAGTCAAACAGAAATCCTCCTACGAGATTGTTGGTGATTTTGTATCCATATTTAGAGACTCAGTATACCACAATGGCTGCTACTCAAAGCTTTACAAGAAGCACCAACGTCTTGGAAAGAGAAAGAAATCAGATTCTGAACCGGGGTTATTGGTGAAAGACAATCAAACTGCTGCAAATGAAGGTGAATCTGGGGAGAGGGACATTAAGAAGAAACGAGTTATGGTGTCTGATAAGCGTACAAAAAAGAAAGCTTCTGAGACACGAAGTGCAAAGAGAAGAAAGCTAAATTCTGTAATTAAGTCGTTGGGAAAAGACAATGAGAATGCAGAAAATCTGCCTGTTAGTGGAAGTGGGCAGAGGAACATCAACAATAACCAAGATAAGCCTAAGAAAAAGCAAGCTGTTGAGCCACCAATTTCAAAGCTGGTGAGGCGAAGATCAAAGCATGCTGAAGGTCGGGATTTGAAGGTTAAGGAGACGGAAGAAGAGGCTTCACCTACATCCCTTTTTGTAATATTTGGCCCTGGATCCTCGCTGCCCACGAAGGATGATCTTCTCAGTATATACGGTAAATTTGGGGAACTAAACGTAACTGAAACTGAGATGTTCTACACCAATTTCTGTGCCCGTGTGGCATTTGTAAGGAGCTCTGATGCAGAAGAAGCCTTCAATCATTCTTTAATCCACAGTCCCTTTGGAGCTTCCAATGTCAATTTCCGGCTCCAAAATCCCTCAGGTGCTTCTAAGACTCGTGAGCTGAGCACATTACCAGAGTCTCCTCCAGCAAAGAAGTCCGAGTCCAAGAGTGCAAGTCGGGCATCAGTAGCTTCAGAAACGCCTGCTGGTAAGCCGTCGCAGGTTGGTTTCATTAGGCATAAACTTGAGATGATGATATCAATGCTGGGAGACTCGGATCACAAAGTGTCACCTGTTGTCACATCCAAATTGAAGAATGAGATAAAGGATCTACTTAAGGTGGTGAGCGCAACGGGTGAATCCTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1109

Amino Acids

119.62

Weight (kDa)

6.05

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 214 - 297 5.8e-21 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 947
AasI GACNNNNNNGTC 1 cut(s) 1690
Acc36I ACCTGC 1 cut(s) 3166
AccB1I GGYRCC 1 cut(s) 786
AccBSI CCGCTC 2 cut(s) 74, 1219
AccI GTMKAC 1 cut(s) 2382
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 68
AciI CCGC 9 cut(s) 74, 90, 92, 123, 141, 200, 381, 1190, 1219
AclWI GGATC 7 cut(s) 692, 2237, 2300, 2841, 2854, 3240, 3291
AcoI YGGCCR 1 cut(s) 680
AcsI RAATTY 6 cut(s) 316, 400, 460, 1550, 2605, 2891
AcuI CTGAAG 5 cut(s) 687, 1029, 1455, 2790, 3132
AdeI CACNNNGTG 2 cut(s) 2585, 3241
AfaI GTAC 3 cut(s) 1444, 1928, 2557
AfiI CCNNNNNNNGG 7 cut(s) 126, 569, 570, 1356, 1797, 2087, 2663
AflII CTTAAG 2 cut(s) 2120, 3290
AflIII ACRYGT 1 cut(s) 227
AhdI GACNNNNNGTC 1 cut(s) 1264
AhlI ACTAGT 1 cut(s) 1100
AjnI CCWGG 4 cut(s) 782, 1977, 2102, 2842
AjuI GAANNNNNNNTTGG 6 cut(s) 1002, 1034, 1571, 1603, 3017, 3049
Alw21I GWGCWC 4 cut(s) 1108, 1116, 2969, 3083
Alw26I GTCTC 7 cut(s) 199, 1566, 2367, 2570, 2789, 3099, 3219
AlwI GGATC 7 cut(s) 692, 2237, 2300, 2841, 2854, 3240, 3291
AlwNI CAGNNNCTG 1 cut(s) 2453
Aor13HI TCCGGA 1 cut(s) 68
AoxI GGCC 7 cut(s) 87, 480, 680, 685, 1012, 1848, 2839
ApoI RAATTY 6 cut(s) 316, 400, 460, 1550, 2605, 2891
AspA2I CCTAGG 1 cut(s) 28
AspLEI GCGC 1 cut(s) 3305
AspS9I GGNCC 5 cut(s) 135, 685, 1879, 2127, 2840
AsuC2I CCSGG 4 cut(s) 52, 596, 684, 2460
AsuII TTCGAA 2 cut(s) 308, 1154
AvaII GGWCC 3 cut(s) 135, 1879, 2127
AvrII CCTAGG 1 cut(s) 28
AxyI CCTNAGG 2 cut(s) 2133, 3052
BaeGI GKGCMC 1 cut(s) 2947
BamHI GGATCC 1 cut(s) 2846
BanI GGYRCC 1 cut(s) 786
BanII GRGCYC 3 cut(s) 1116, 1898, 2969
BauI CACGAG 2 cut(s) 2005, 3069
BbsI GAAGAC 5 cut(s) 317, 597, 1131, 1260, 1688
Bbv12I GWGCWC 4 cut(s) 1108, 1116, 2969, 3083
BccI CCATC 7 cut(s) 256, 434, 798, 1219, 1631, 1835, 2177
BceAI ACGGC 3 cut(s) 394, 1249, 3154
BciT130I CCWGG 4 cut(s) 784, 1979, 2104, 2844
BciVI GTATCC 2 cut(s) 217, 2371
BcnI CCSGG 4 cut(s) 52, 596, 684, 2460
BcoDI GTCTC 7 cut(s) 199, 1566, 2367, 2570, 2789, 3099, 3219
BcuI ACTAGT 1 cut(s) 1100
BfaI CTAG 6 cut(s) 29, 78, 363, 1101, 1238, 1751
BfmI CTRYAG 1 cut(s) 21
BfrI CTTAAG 2 cut(s) 2120, 3290
BfuAI ACCTGC 1 cut(s) 3166
BfuI GTATCC 2 cut(s) 217, 2371
BglI GCCNNNNNGGC 1 cut(s) 2951
BglII AGATCT 1 cut(s) 17
BlnI CCTAGG 1 cut(s) 28
BlpI GCTNAGC 1 cut(s) 3077
BmcAI AGTACT 1 cut(s) 1928
Bme1390I CCNGG 8 cut(s) 52, 596, 684, 784, 1979, 2104, 2460, 2844
Bme18I GGWCC 3 cut(s) 135, 1879, 2127
BmeRI GACNNNNNGTC 1 cut(s) 1264
BmgT120I GGNCC 5 cut(s) 135, 685, 1879, 2127, 2840
BmrFI CCNGG 8 cut(s) 52, 596, 684, 784, 1979, 2104, 2460, 2844
BmsI GCATC 6 cut(s) 313, 697, 980, 1735, 2962, 3146
BpiI GAAGAC 5 cut(s) 317, 597, 1131, 1260, 1688
BpmI CTGGAG 5 cut(s) 835, 1002, 2084, 2304, 3084
Bpu10I CCTNAGC 1 cut(s) 967
Bpu1102I GCTNAGC 1 cut(s) 3077
Bpu14I TTCGAA 2 cut(s) 308, 1154
BpuEI CTTGAG 2 cut(s) 1130, 3221
BpuMI CCSGG 4 cut(s) 52, 596, 684, 2460
Bsa29I ATCGAT 1 cut(s) 2177
BsaAI YACGTR 1 cut(s) 228
BsaJI CCNNGG 9 cut(s) 28, 90, 357, 417, 674, 738, 2103, 2459, 2842
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 569, 570, 1356, 1797, 2087, 2663
Bse118I RCCGGY 1 cut(s) 1048
Bse1I ACTGG 4 cut(s) 852, 1294, 1473, 2318
Bse21I CCTNAGG 2 cut(s) 2133, 3052
Bse3DI GCAATG 1 cut(s) 1371
BseAI TCCGGA 1 cut(s) 68
BseBI CCWGG 4 cut(s) 784, 1979, 2104, 2844
BseCI ATCGAT 1 cut(s) 2177
BseDI CCNNGG 9 cut(s) 28, 90, 357, 417, 674, 738, 2103, 2459, 2842
BseLI CCNNNNNNNGG 7 cut(s) 126, 569, 570, 1356, 1797, 2087, 2663
BseMI GCAATG 1 cut(s) 1371
BseNI ACTGG 4 cut(s) 852, 1294, 1473, 2318
BseRI GAGGAG 5 cut(s) 110, 301, 404, 524, 3087
BseSI GKGCMC 1 cut(s) 2947
BseYI CCCAGC 1 cut(s) 3220
BsgI GTGCAG 2 cut(s) 893, 1262
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 7 cut(s) 89, 482, 682, 687, 1014, 1850, 2841
BshNI GGYRCC 1 cut(s) 786
BshVI ATCGAT 1 cut(s) 2177
BsiHKAI GWGCWC 4 cut(s) 1108, 1116, 2969, 3083
BsiSI CCGG 9 cut(s) 51, 69, 120, 138, 595, 683, 1049, 2459, 3037
BslFI GGGAC 6 cut(s) 19, 586, 989, 2113, 2531, 2994
BslI CCNNNNNNNGG 7 cut(s) 126, 569, 570, 1356, 1797, 2087, 2663
BsmAI GTCTC 7 cut(s) 199, 1566, 2367, 2570, 2789, 3099, 3219
BsmBI CGTCTC 1 cut(s) 2789
BsmFI GGGAC 6 cut(s) 19, 586, 989, 2113, 2531, 2994
BsmI GAATGC 2 cut(s) 982, 2647
BsnI GGCC 7 cut(s) 89, 482, 682, 687, 1014, 1850, 2841
Bsp119I TTCGAA 2 cut(s) 308, 1154
Bsp1286I GDGCHC 6 cut(s) 1108, 1116, 1898, 2947, 2969, 3083
Bsp13I TCCGGA 1 cut(s) 68
Bsp1720I GCTNAGC 1 cut(s) 3077
BspACI CCGC 9 cut(s) 74, 90, 92, 123, 141, 200, 381, 1190, 1219
BspANI GGCC 7 cut(s) 89, 482, 682, 687, 1014, 1850, 2841
BspDI ATCGAT 1 cut(s) 2177
BspEI TCCGGA 1 cut(s) 68
BspFNI CGCG 1 cut(s) 92
BspMI ACCTGC 1 cut(s) 3166
BspPI GGATC 7 cut(s) 692, 2237, 2300, 2841, 2854, 3240, 3291
BspQI GCTCTTC 1 cut(s) 1361
BspT104I TTCGAA 2 cut(s) 308, 1154
BspT107I GGYRCC 1 cut(s) 786
BspTI CTTAAG 2 cut(s) 2120, 3290
BsrBI CCGCTC 2 cut(s) 74, 1219
BsrDI GCAATG 1 cut(s) 1371
BsrFI RCCGGY 1 cut(s) 1048
BsrI ACTGG 4 cut(s) 852, 1294, 1473, 2318
BssAI RCCGGY 1 cut(s) 1048
BssECI CCNNGG 9 cut(s) 28, 90, 357, 417, 674, 738, 2103, 2459, 2842
BssNAI GTATAC 1 cut(s) 2383
BssSI CACGAG 2 cut(s) 2005, 3069
BssT1I CCWWGG 4 cut(s) 28, 357, 417, 738
Bst1107I GTATAC 1 cut(s) 2383
Bst2BI CACGAG 2 cut(s) 2005, 3069
Bst2UI CCWGG 4 cut(s) 784, 1979, 2104, 2844
Bst4CI ACNGT 8 cut(s) 181, 1184, 1631, 1692, 1733, 1835, 2888, 3008
Bst6I CTCTTC 6 cut(s) 600, 933, 1361, 1659, 1700, 2799
BstAFI CTTAAG 2 cut(s) 2120, 3290
BstAPI GCANNNNNTGC 2 cut(s) 977, 1088
BstBAI YACGTR 1 cut(s) 228
BstBI TTCGAA 2 cut(s) 308, 1154
BstC8I GCNNGC 6 cut(s) 720, 879, 928, 2718, 2766, 3159
BstDSI CCRYGG 2 cut(s) 90, 674
BstEII GGTNACC 1 cut(s) 1320
BstENI CCTNNNNNAGG 1 cut(s) 1795
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 3305
BstMAI GTCTC 7 cut(s) 199, 1566, 2367, 2570, 2789, 3099, 3219
BstMWI GCNNNNNNNGC 9 cut(s) 977, 1088, 1589, 2261, 2749, 2951, 2981, 3134, 3143
BstNI CCWGG 4 cut(s) 784, 1979, 2104, 2844
BstNSI RCATGY 3 cut(s) 722, 1264, 2768
BstPI GGTNACC 1 cut(s) 1320
BstSCI CCNGG 8 cut(s) 50, 594, 682, 782, 1977, 2102, 2458, 2842
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 2947
BstUI CGCG 1 cut(s) 92
BstV2I GAAGAC 5 cut(s) 317, 597, 1131, 1260, 1688
BstX2I RGATCY 3 cut(s) 17, 2846, 3283
BstXI CCANNNNNNTGG 1 cut(s) 2429
BstYI RGATCY 3 cut(s) 17, 2846, 3283
BstZ17I GTATAC 1 cut(s) 2383
Bsu15I ATCGAT 1 cut(s) 2177
Bsu36I CCTNAGG 2 cut(s) 2133, 3052
BsuI GTATCC 2 cut(s) 217, 2371
BsuRI GGCC 7 cut(s) 89, 482, 682, 687, 1014, 1850, 2841
BsuTUI ATCGAT 1 cut(s) 2177
BtgI CCRYGG 2 cut(s) 90, 674
BtsI GCAGTG 2 cut(s) 1250, 2158
BtsIMutI CAGTG 6 cut(s) 859, 1189, 1250, 1688, 1725, 2158
BveI ACCTGC 1 cut(s) 3166
Cac8I GCNNGC 6 cut(s) 720, 879, 928, 2718, 2766, 3159
CaiI CAGNNNCTG 1 cut(s) 2453
CfoI GCGC 1 cut(s) 3305
Cfr10I RCCGGY 1 cut(s) 1048
Cfr13I GGNCC 5 cut(s) 135, 685, 1879, 2127, 2840
Cfr42I CCGCGG 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 2177
Csp6I GTAC 3 cut(s) 1443, 1927, 2556
CviAII CATG 8 cut(s) 719, 931, 1261, 1417, 1505, 1873, 2012, 2765
CviQI GTAC 3 cut(s) 1443, 1927, 2556
DraIII CACNNNGTG 2 cut(s) 2585, 3241
DrdI GACNNNNNNGTC 1 cut(s) 1690
DriI GACNNNNNGTC 1 cut(s) 1264
DseDI GACNNNNNNGTC 1 cut(s) 1690
EaeI YGGCCR 1 cut(s) 680
Eam1104I CTCTTC 6 cut(s) 600, 933, 1361, 1659, 1700, 2799
Eam1105I GACNNNNNGTC 1 cut(s) 1264
EarI CTCTTC 6 cut(s) 600, 933, 1361, 1659, 1700, 2799
EciI GGCGGA 1 cut(s) 156
Ecl136II GAGCTC 2 cut(s) 1114, 2967
Eco130I CCWWGG 4 cut(s) 28, 357, 417, 738
Eco24I GRGCYC 3 cut(s) 1116, 1898, 2969
Eco32I GATATC 1 cut(s) 3213
Eco47I GGWCC 3 cut(s) 135, 1879, 2127
Eco53kI GAGCTC 2 cut(s) 1114, 2967
Eco57I CTGAAG 5 cut(s) 687, 1029, 1455, 2790, 3132
Eco81I CCTNAGG 2 cut(s) 2133, 3052
Eco91I GGTNACC 1 cut(s) 1320
EcoICRI GAGCTC 2 cut(s) 1114, 2967
EcoNI CCTNNNNNAGG 1 cut(s) 1795
EcoO109I RGGNCCY 1 cut(s) 2127
EcoO65I GGTNACC 1 cut(s) 1320
EcoRI GAATTC 3 cut(s) 316, 400, 460
EcoRII CCWGG 4 cut(s) 782, 1977, 2102, 2842
EcoRV GATATC 1 cut(s) 3213
EcoT14I CCWWGG 4 cut(s) 28, 357, 417, 738
EcoT38I GRGCYC 3 cut(s) 1116, 1898, 2969
ErhI CCWWGG 4 cut(s) 28, 357, 417, 738
Esp3I CGTCTC 1 cut(s) 2789
FaeI CATG 8 cut(s) 722, 934, 1264, 1420, 1508, 1876, 2015, 2768
FalI AAGNNNNNCTT 2 cut(s) 1775, 1807
FaqI GGGAC 6 cut(s) 19, 586, 989, 2113, 2531, 2994
FatI CATG 8 cut(s) 718, 930, 1260, 1416, 1504, 1872, 2011, 2764
FauI CCCGC 2 cut(s) 116, 193
FblI GTMKAC 1 cut(s) 2382
FriOI GRGCYC 3 cut(s) 1116, 1898, 2969
FspBI CTAG 6 cut(s) 29, 78, 363, 1101, 1238, 1751
GlaI GCGC 1 cut(s) 3304
GsaI CCCAGC 1 cut(s) 3224
GsuI CTGGAG 5 cut(s) 835, 1002, 2084, 2304, 3084
HaeIII GGCC 7 cut(s) 89, 482, 682, 687, 1014, 1850, 2841
HapII CCGG 9 cut(s) 51, 69, 120, 138, 595, 683, 1049, 2459, 3037
HhaI GCGC 1 cut(s) 3305
Hin1II CATG 8 cut(s) 722, 934, 1264, 1420, 1508, 1876, 2015, 2768
Hin6I GCGC 1 cut(s) 3303
HinP1I GCGC 1 cut(s) 3303
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 2 cut(s) 2405, 2568
HpaII CCGG 9 cut(s) 51, 69, 120, 138, 595, 683, 1049, 2459, 3037
Hpy166II GTNNAC 7 cut(s) 376, 436, 496, 1054, 1180, 1187, 2383
Hpy188III TCNNGA 8 cut(s) 69, 1109, 1208, 2005, 2063, 2283, 2777, 3071
Hpy8I GTNNAC 7 cut(s) 376, 436, 496, 1054, 1180, 1187, 2383
Hpy99I CGWCG 2 cut(s) 799, 3175
HpyCH4III ACNGT 8 cut(s) 181, 1184, 1631, 1692, 1733, 1835, 2888, 3008
HpyCH4IV ACGT 4 cut(s) 8, 227, 2425, 2907
HpyF10VI GCNNNNNNNGC 9 cut(s) 977, 1088, 1589, 2261, 2749, 2951, 2981, 3134, 3143
HpySE526I ACGT 4 cut(s) 8, 227, 2425, 2907
Hsp92II CATG 8 cut(s) 722, 934, 1264, 1420, 1508, 1876, 2015, 2768
HspAI GCGC 1 cut(s) 3303
KflI GGGWCCC 1 cut(s) 2127
Kpn2I TCCGGA 1 cut(s) 68
KspI CCGCGG 1 cut(s) 93
LguI GCTCTTC 1 cut(s) 1361
LweI GCATC 6 cut(s) 313, 697, 980, 1735, 2962, 3146
MaeI CTAG 6 cut(s) 29, 78, 363, 1101, 1238, 1751
MaeII ACGT 4 cut(s) 8, 227, 2425, 2907
MbiI CCGCTC 2 cut(s) 74, 1219
MflI RGATCY 3 cut(s) 17, 2846, 3283
MhlI GDGCHC 6 cut(s) 1108, 1116, 1898, 2947, 2969, 3083
MmeI TCCRAC 1 cut(s) 235
MroI TCCGGA 1 cut(s) 68
MslI CAYNNNNRTG 1 cut(s) 1421
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 2 cut(s) 2120, 3290
MspI CCGG 9 cut(s) 51, 69, 120, 138, 595, 683, 1049, 2459, 3037
MspR9I CCNGG 8 cut(s) 52, 596, 684, 784, 1979, 2104, 2460, 2844
Mva1269I GAATGC 2 cut(s) 982, 2647
MvaI CCWGG 4 cut(s) 784, 1979, 2104, 2844
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 9 cut(s) 977, 1088, 1589, 2261, 2749, 2951, 2981, 3134, 3143
NciI CCSGG 4 cut(s) 52, 596, 684, 2460
NlaIII CATG 8 cut(s) 722, 934, 1264, 1420, 1508, 1876, 2015, 2768
NmeAIII GCCGAG 2 cut(s) 176, 1040
NmuCI GTSAC 7 cut(s) 614, 1320, 1932, 1952, 2002, 3243, 3253
NspI RCATGY 3 cut(s) 722, 1264, 2768
NspV TTCGAA 2 cut(s) 308, 1154
PaeI GCATGC 2 cut(s) 722, 2768
PciSI GCTCTTC 1 cut(s) 1361
PcsI WCGNNNNNNNCGW 1 cut(s) 2858
PctI GAATGC 2 cut(s) 982, 2647
PfeI GAWTC 9 cut(s) 425, 485, 1522, 1623, 1988, 2143, 2450, 2504, 3314
Ppu21I YACGTR 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 2127
PsiI TTATAA 1 cut(s) 947
Psp124BI GAGCTC 2 cut(s) 1116, 2969
Psp5II RGGWCCY 1 cut(s) 2127
Psp6I CCWGG 4 cut(s) 782, 1977, 2102, 2842
PspEI GGTNACC 1 cut(s) 1320
PspFI CCCAGC 1 cut(s) 3220
PspGI CCWGG 4 cut(s) 782, 1977, 2102, 2842
PspPI GGNCC 5 cut(s) 135, 685, 1879, 2127, 2840
PspPPI RGGWCCY 1 cut(s) 2127
PstNI CAGNNNCTG 1 cut(s) 2453
PsuI RGATCY 3 cut(s) 17, 2846, 3283
RsaI GTAC 3 cut(s) 1444, 1928, 2557
RsaNI GTAC 3 cut(s) 1443, 1927, 2556
RseI CAYNNNNRTG 1 cut(s) 1421
SacI GAGCTC 2 cut(s) 1116, 2969
SacII CCGCGG 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 1361
Sau96I GGNCC 5 cut(s) 135, 685, 1879, 2127, 2840
ScaI AGTACT 1 cut(s) 1928
ScrFI CCNGG 8 cut(s) 52, 596, 684, 784, 1979, 2104, 2460, 2844
SduI GDGCHC 6 cut(s) 1108, 1116, 1898, 2947, 2969, 3083
SfaNI GCATC 6 cut(s) 313, 697, 980, 1735, 2962, 3146
SfcI CTRYAG 1 cut(s) 21
Sfr303I CCGCGG 1 cut(s) 93
SfuI TTCGAA 2 cut(s) 308, 1154
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 3 cut(s) 135, 1879, 2127
SmiMI CAYNNNNRTG 1 cut(s) 1421
SmlI CTYRAG 4 cut(s) 1109, 2120, 3200, 3290
SmoI CTYRAG 4 cut(s) 1109, 2120, 3200, 3290
SpeI ACTAGT 1 cut(s) 1100
SphI GCATGC 2 cut(s) 722, 2768
SsiI CCGC 9 cut(s) 74, 90, 92, 123, 141, 200, 381, 1190, 1219
SspI AATATT 2 cut(s) 821, 2835
SspMI CTAG 6 cut(s) 29, 78, 363, 1101, 1238, 1751
SstI GAGCTC 2 cut(s) 1116, 2969
StyD4I CCNGG 8 cut(s) 50, 594, 682, 782, 1977, 2102, 2458, 2842
StyI CCWWGG 4 cut(s) 28, 357, 417, 738
TaaI ACNGT 8 cut(s) 181, 1184, 1631, 1692, 1733, 1835, 2888, 3008
TaiI ACGT 4 cut(s) 11, 230, 2428, 2910
TaqI TCGA 5 cut(s) 308, 974, 1154, 1904, 2177
TatI WGTACW 1 cut(s) 1926
TauI GCSGC 2 cut(s) 77, 92
TfiI GAWTC 9 cut(s) 425, 485, 1522, 1623, 1988, 2143, 2450, 2504, 3314
TscAI CASTG 6 cut(s) 859, 1189, 1250, 1695, 1732, 2158
TseFI GTSAC 7 cut(s) 614, 1320, 1932, 1952, 2002, 3243, 3253
Tsp45I GTSAC 7 cut(s) 614, 1320, 1932, 1952, 2002, 3243, 3253
TspGWI ACGGA 4 cut(s) 633, 783, 1784, 2813
TspRI CASTG 6 cut(s) 859, 1189, 1250, 1695, 1732, 2158
Vha464I CTTAAG 2 cut(s) 2120, 3290
VpaK11BI GGWCC 3 cut(s) 135, 1879, 2127
XagI CCTNNNNNAGG 1 cut(s) 1795
XapI RAATTY 6 cut(s) 316, 400, 460, 1550, 2605, 2891
XceI RCATGY 3 cut(s) 722, 1264, 2768
XcmI CCANNNNNNNNNTGG 1 cut(s) 3011
XmaJI CCTAGG 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 2382
XspI CTAG 6 cut(s) 29, 78, 363, 1101, 1238, 1751
ZrmI AGTACT 1 cut(s) 1928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.