MD09G1031100.v1.1

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002210
Physical Location & Seq
Forward (+)
1886077 .. 1889613
3537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1031100.v1.1.491

Sequence Viewer

Length: 2829 bp
ATGGGGATGTTAGGGACGCGATCTACTACATCCCTGGAGGAGGATTTGGGTTCTGCACCTGAACCGGATAAGCATGAAGTTCCGGAGGAGAAGACGATCGGGGAAGTTGTGACCGACTCGGGAGGGTCCGGTCGGGGAAGTTCCGGCGGTGGAAGGAGTTTTGGGAACCTGAGTGTTGATGTCTCGGATGCTGAGGAGGGCTTGGTGAAGCCGAGAGGGAAAGGGAGAGTTGAGAAGTTGGAGTCTGGTTCGAGTAACGAGAAGAAGGCGGTGAGAGGCGGTGCTGAGGTTGATCATGGTGAGAACGGAGGAGGGGTTGGAGAAAATGGGAGTTCTTTGGATGGAATTGGAGAAGGCCCGGATGGGACTGAGATTATTGAGACCGGGACCGATGTGAACGGAGGGGTTGAAGAGAATGGGAGCTGTTTGGATGGGACTGGAGAAGACCCGGATGAGAAAACTGATGAGATTAGTGAGGACATGGATGATGAGGGACATGAGTTTCTTGTTGGTGATTTTGTGTGGGGAAAGATTAAGAGCCATCCGTGGTGGCCTGCACAAATTTGTGATCCTTTGGACGCTTCGGAGTATGCCCTGAAATTGAAGGCCAAAGACAGACTCCTGGTGGCGTATTTTGGGGACGGAACATTTGCTTGGTGCAATTCATTGCAGCTGAAACCGTTTGAGGAGAACTTTAGGGAGATGTCGGAGCAGAGTAGCTCGAAGGCGTTTGTTAATGCTGTGCAGCAGGCTGTGGATGAGGTTGGAAGGATTGTGATGTTGAAGATGAGTTGTGGGTGTGTAAAGGAAAAGTTCCTGAGTGAGGTTGGTCGGCCGTTGGCAGTAAATGCCGGAATTAAGGAAGGAGTTCTTGTGCCAGAAGGCAGGGTTGGGAAGCTTTTGGATCGTCTGTGTGAGCCGGCAGAGCTTCTTGCTGAGTTGAAACATGTTGCAGAAGTTATGTCCACGTCTAGTGAGCTTCAGCTGAATGCTTTGAAGAGTTGGTTGTCGGCATTTTATTGTTCAAAAGGGGGCTATCATTTGCCTGTTTTCGTCGAAGCGCAGCCAGTTCCTGGTCTTGAGGATGATTGGAGGGCGGTGGATGTCCCGCTCCAAGGGCCATTTGAAGACTGGCTTTCTTCTCCCAGAAAATCAGGTCAAACTGATCAACCTTTACATGAAAATTCAGCTCAAGGTTTAGAGAATAGGCAATACCAAAGAAGGAAGCAGAAGAGCATTGCTGATCTAATGGGAGAGTATGATGATATTCAGGTGGAAACAAAGGAAGGAGCAACATCAGAGAAAGCAGGGGTATCATCTGGGCGGAAGAAGCGGAAAGTTGGCGAGAATCATGGTGAGAGTAATTTGACTTCTGAATCCAGAAAGAGAAGGGCCAAGCTTTCAAAGACACCAATGAGCACGCAGATGAAAAAACTATCATGTGTTGAAAATGGTTCTAGTGGCCGTAAGGAAGAAACTAAGAAGGGTGCCTTAACAAGGAGCAGGAAGAAGGCTGAAGGTACTGGAATTGAAAGCAATGGTGGCGAGACCAAAGAAGAAGCTGGTGATAGTCCAATTTTGCGCAGTGGCGGTTCACAAACTGACATGAAAGACCAAATTGATCACGCCTTTTCCACGAGAGAGAGGAAAAGGAGCAAGTACTTGTCCCCTCCCTTCATAAATCTAAGCACTGGAAAAAGGAGTCTAGACATGGAAGTAGAATCTCTGAAAGTTTATAATGAGAACCTTGTTGGGTCGCCGAAGATGCTGAATCCTTGCATGGAGACGTTACAGAAGAAAGACTCTACAGAACTTGGTCTTGGGAATGAGATATCTGGTGGCTCGAGTTCGAAAAAACCATCAGCAGATGACAAGAAGAGCATTGATCAGATGAAAGCTAATGTATCTAATCGTAACGTGCTATCTGGAGTCCGCTCTGCAGCTGTTAATCCATCATCTCCAATAAAAAAGAAATCCTTTGAGATTGTTAAGGATTTTCTGTCCGTATTCAGAGACTCGATTTATCGCAGTGGATCCTACTACGACATATACAAGAAGAAGCAACCTGATAAGAAGAGAAAGAAGTTAGAATCTGAACCTGGCTCATTGGGGAAAGACCGAAATCAGACTGCAGAAAACCTGCCCGAAACTGAATCTGGGAAGAAGAGAATCAAGAAGAGCAGTGAGACAAAGTCTGCTAAGTCTACACAAAAGCAAGCTACTGAGACACCAGGTTCAGAGCCTGGTAGTAAGCGAAAATCAAAGCATGCTTCTGGAACTCCGGATTTGAAGAAAAGGCGTAGGAAGACTGACGAAATAGCTTCACCTGCATCCCTTTTTGTGACATTTGGCCCTGGATCCAATCTCCCCACAAAAGCTGATCTTATCAAGATTTATAGTAAGTTCGGAGAGCTGAACGAAACAGAAACTGAGATGTTCTACACCAATTTCTGCGCTCGTGTTTCCTTTGCAAGGCTAGCCGATGCACAAGAAGCCTTCAACCATTCGCAAAACGACAGTCCGTTTGGAGCTTCCAACGTCAATTTCCGGCTTCATAATCTGGCAGCTGCTTCGAAGGTTCGTGAACTGAGTGAAATATCCAATTCTGCTCCCGCTAAGAAGTCCAGGGGCAAGACCAAAACCCAGGCATTAGCTTCACAGGCACCCGCTGCTGCTGGTGAGGCGTCGCAGATCGACTTAATCAAGCAGAAACTGGAGAAGATGACCTCAATGCTGGATGATTCAAATGGCCAAGTGTCGGATGTCACGAAATCGAAACTGGAGAGCGAGATAAAAGAGCTGTTGGGGGCGGTAAGCACGATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

943

Amino Acids

102.25

Weight (kDa)

6.43

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 171 - 254 8e-21 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1737
AarI CACCTGC 1 cut(s) 2335
Acc16I TGCGCA 1 cut(s) 1583
Acc36I ACCTGC 2 cut(s) 2148, 2335
AccB1I GGYRCC 2 cut(s) 1487, 2662
AccB7I CCANNNNNTGG 1 cut(s) 1073
AccBSI CCGCTC 2 cut(s) 1111, 1935
AccI GTMKAC 1 cut(s) 2204
AccII CGCG 1 cut(s) 19
AccIII TCCGGA 2 cut(s) 82, 2281
AclWI GGATC 6 cut(s) 563, 912, 2028, 2041, 2352, 2365
AcoI YGGCCR 3 cut(s) 833, 1462, 2749
AcsI RAATTY 2 cut(s) 561, 1183
AcuI CTGAAG 2 cut(s) 965, 1536
AcyI GRCGYC 1 cut(s) 2684
AfaI GTAC 2 cut(s) 1522, 1661
AfiI CCNNNNNNNGG 7 cut(s) 40, 823, 1073, 1115, 1497, 2472, 2758
AflIII ACRYGT 1 cut(s) 946
AjiI CACGTC 1 cut(s) 969
AjnI CCWGG 9 cut(s) 33, 621, 1072, 2098, 2230, 2242, 2353, 2624, 2643
AjuI GAANNNNNNNTTGG 8 cut(s) 637, 669, 873, 905, 1803, 1835, 2528, 2560
Alw21I GWGCWC 1 cut(s) 1421
Alw26I GTCTC 7 cut(s) 187, 374, 1541, 1778, 2007, 2180, 2219
AlwI GGATC 6 cut(s) 563, 912, 2028, 2041, 2352, 2365
AlwNI CAGNNNCTG 4 cut(s) 1073, 2243, 2429, 2713
Ama87I CYCGRG 2 cut(s) 118, 1843
Aor13HI TCCGGA 2 cut(s) 82, 2281
AoxI GGCC 9 cut(s) 355, 551, 606, 833, 1118, 1392, 1462, 2350, 2749
ApeKI GCWGC 8 cut(s) 670, 745, 1063, 1940, 2564, 2567, 2669, 2672
ApoI RAATTY 2 cut(s) 561, 1183
ArsI GACNNNNNNTTYG 6 cut(s) 632, 664, 2503, 2507, 2535, 2539
Asp700I GAANNNNTTC 1 cut(s) 867
AspLEI GCGC 3 cut(s) 1063, 1584, 2456
AspS9I GGNCC 6 cut(s) 126, 356, 387, 1118, 1392, 2351
AsuC2I CCSGG 3 cut(s) 359, 385, 449
AsuHPI GGTGA 8 cut(s) 217, 283, 311, 524, 1367, 1577, 2316, 2690
AsuII TTCGAA 2 cut(s) 1850, 2573
AsuNHI GCTAGC 1 cut(s) 2476
AvaI CYCGRG 2 cut(s) 118, 1843
AvaII GGWCC 2 cut(s) 126, 387
BalI TGGCCA 1 cut(s) 2751
BamHI GGATCC 2 cut(s) 2033, 2357
BanI GGYRCC 2 cut(s) 1487, 2662
BarI GAAGNNNNNNTAC 2 cut(s) 1354, 1386
BauI CACGAG 2 cut(s) 1636, 2457
BbsI GAAGAC 4 cut(s) 98, 450, 1134, 2312
Bbv12I GWGCWC 1 cut(s) 1421
BbvCI CCTCAGC 2 cut(s) 192, 285
BbvI GCAGC 8 cut(s) 682, 757, 1075, 1952, 2554, 2576, 2656, 2659
BccI CCATC 7 cut(s) 335, 356, 425, 549, 1867, 1960, 2815
BceAI ACGGC 2 cut(s) 820, 1449
BcgI CGANNNNNNTGC 2 cut(s) 2553, 2587
BciT130I CCWGG 9 cut(s) 35, 623, 1074, 2100, 2232, 2244, 2355, 2626, 2645
BclI TGATCA 4 cut(s) 292, 1165, 1621, 1885
BcnI CCSGG 3 cut(s) 359, 385, 449
BcoDI GTCTC 7 cut(s) 187, 374, 1541, 1778, 2007, 2180, 2219
BfaI CTAG 4 cut(s) 972, 1458, 1706, 2477
BfmI CTRYAG 3 cut(s) 1806, 1938, 2130
BfuAI ACCTGC 2 cut(s) 2148, 2335
BisI GCNGC 8 cut(s) 671, 746, 1064, 1941, 2565, 2568, 2670, 2673
BlsI GCNGC 8 cut(s) 672, 747, 1065, 1942, 2566, 2569, 2671, 2674
BmcAI AGTACT 1 cut(s) 1661
Bme18I GGWCC 2 cut(s) 126, 387
BmeT110I CYCGRG 2 cut(s) 118, 1843
BmgBI CACGTC 1 cut(s) 969
BmgT120I GGNCC 6 cut(s) 126, 356, 387, 1118, 1392, 2351
BmiI GGNNCC 7 cut(s) 127, 167, 388, 1489, 2035, 2359, 2664
BmsI GCATC 4 cut(s) 178, 1755, 2339, 2473
BmtI GCTAGC 1 cut(s) 2480
BpiI GAAGAC 4 cut(s) 98, 450, 1134, 2312
BpmI CTGGAG 5 cut(s) 56, 459, 1947, 2735, 2801
Bpu10I CCTNAGC 2 cut(s) 192, 285
Bpu14I TTCGAA 2 cut(s) 1850, 2573
BpuEI CTTGAG 2 cut(s) 1100, 1176
BpuMI CCSGG 3 cut(s) 359, 385, 449
BsaHI GRCGYC 1 cut(s) 2684
BsaI GGTCTC 2 cut(s) 374, 1541
BsaJI CCNNGG 6 cut(s) 33, 545, 1114, 2353, 2625, 2643
BsaWI WCCGGW 4 cut(s) 64, 82, 128, 2281
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 858, 888
Bsc4I CCNNNNNNNGG 7 cut(s) 40, 823, 1073, 1115, 1497, 2472, 2758
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 7 cut(s) 442, 1067, 1136, 1528, 1696, 2718, 2784
Bse3DI GCAATG 3 cut(s) 665, 1236, 1543
BseAI TCCGGA 2 cut(s) 82, 2281
BseBI CCWGG 9 cut(s) 35, 623, 1074, 2100, 2232, 2244, 2355, 2626, 2645
BseDI CCNNGG 6 cut(s) 33, 545, 1114, 2353, 2625, 2643
BseLI CCNNNNNNNGG 7 cut(s) 40, 823, 1073, 1115, 1497, 2472, 2758
BseMI GCAATG 3 cut(s) 665, 1236, 1543
BseMII CTCAG 9 cut(s) 161, 183, 276, 360, 809, 927, 2214, 2421, 2579
BseNI ACTGG 7 cut(s) 442, 1067, 1136, 1528, 1696, 2718, 2784
BseRI GAGGAG 5 cut(s) 53, 101, 209, 324, 701
BseX3I CGGCCG 1 cut(s) 833
BseXI GCAGC 8 cut(s) 682, 757, 1075, 1952, 2554, 2576, 2656, 2659
BsgI GTGCAG 3 cut(s) 39, 540, 764
Bsh1236I CGCG 1 cut(s) 19
Bsh1285I CGRYCG 3 cut(s) 99, 133, 836
BshFI GGCC 9 cut(s) 357, 553, 608, 835, 1120, 1394, 1464, 2352, 2751
BshNI GGYRCC 2 cut(s) 1487, 2662
BsiEI CGRYCG 3 cut(s) 99, 133, 836
BsiHKAI GWGCWC 1 cut(s) 1421
BsiHKCI CYCGRG 2 cut(s) 118, 1843
BslFI GGGAC 8 cut(s) 28, 379, 400, 448, 507, 653, 1091, 1651
BslI CCNNNNNNNGG 7 cut(s) 40, 823, 1073, 1115, 1497, 2472, 2758
BsmAI GTCTC 7 cut(s) 187, 374, 1541, 1778, 2007, 2180, 2219
BsmBI CGTCTC 1 cut(s) 1778
BsmFI GGGAC 8 cut(s) 28, 379, 400, 448, 507, 653, 1091, 1651
BsmI GAATGC 1 cut(s) 994
BsnI GGCC 9 cut(s) 357, 553, 608, 835, 1120, 1394, 1464, 2352, 2751
Bso31I GGTCTC 2 cut(s) 374, 1541
BsoBI CYCGRG 2 cut(s) 118, 1843
Bsp119I TTCGAA 2 cut(s) 1850, 2573
Bsp1286I GDGCHC 1 cut(s) 1421
Bsp13I TCCGGA 2 cut(s) 82, 2281
BspANI GGCC 9 cut(s) 357, 553, 608, 835, 1120, 1394, 1464, 2352, 2751
BspCNI CTCAG 9 cut(s) 162, 184, 277, 361, 810, 928, 2215, 2422, 2580
BspEI TCCGGA 2 cut(s) 82, 2281
BspFNI CGCG 1 cut(s) 19
BspLI GGNNCC 7 cut(s) 127, 167, 388, 1489, 2035, 2359, 2664
BspMAI CTGCAG 2 cut(s) 1942, 2134
BspMI ACCTGC 2 cut(s) 2148, 2335
BspOI GCTAGC 1 cut(s) 2480
BspPI GGATC 6 cut(s) 563, 912, 2028, 2041, 2352, 2365
BspQI GCTCTTC 3 cut(s) 1226, 1871, 2171
BspT104I TTCGAA 2 cut(s) 1850, 2573
BspT107I GGYRCC 2 cut(s) 1487, 2662
BspTNI GGTCTC 2 cut(s) 374, 1541
BsrBI CCGCTC 2 cut(s) 1111, 1935
BsrDI GCAATG 3 cut(s) 665, 1236, 1543
BsrFI RCCGGY 1 cut(s) 919
BsrI ACTGG 7 cut(s) 442, 1067, 1136, 1528, 1696, 2718, 2784
BssAI RCCGGY 1 cut(s) 919
BssECI CCNNGG 6 cut(s) 33, 545, 1114, 2353, 2625, 2643
BssNI GRCGYC 1 cut(s) 2684
BssSI CACGAG 2 cut(s) 1636, 2457
BssT1I CCWWGG 1 cut(s) 1114
Bst2BI CACGAG 2 cut(s) 1636, 2457
Bst2UI CCWGG 9 cut(s) 35, 623, 1074, 2100, 2232, 2244, 2355, 2626, 2645
Bst4CI ACNGT 2 cut(s) 681, 2519
Bst6I CTCTTC 7 cut(s) 405, 992, 1226, 1871, 2069, 2159, 2171
BstACI GRCGYC 1 cut(s) 2684
BstAPI GCANNNNNTGC 2 cut(s) 848, 2669
BstBI TTCGAA 2 cut(s) 1850, 2573
BstC8I GCNNGC 7 cut(s) 555, 750, 921, 1421, 2217, 2268, 2478
BstDSI CCRYGG 1 cut(s) 545
BstENI CCTNNNNNAGG 4 cut(s) 38, 821, 1495, 2470
BstFNI CGCG 1 cut(s) 19
BstHHI GCGC 3 cut(s) 1063, 1584, 2456
BstMAI GTCTC 7 cut(s) 187, 374, 1541, 1778, 2007, 2180, 2219
BstMCI CGRYCG 3 cut(s) 99, 133, 836
BstNI CCWGG 9 cut(s) 35, 623, 1074, 2100, 2232, 2244, 2355, 2626, 2645
BstNSI RCATGY 2 cut(s) 950, 2270
BstSFI CTRYAG 3 cut(s) 1806, 1938, 2130
BstUI CGCG 1 cut(s) 19
BstV1I GCAGC 8 cut(s) 682, 757, 1075, 1952, 2554, 2576, 2656, 2659
BstV2I GAAGAC 4 cut(s) 98, 450, 1134, 2312
BstX2I RGATCY 2 cut(s) 2033, 2357
BstYI RGATCY 2 cut(s) 2033, 2357
BstZI CGGCCG 1 cut(s) 833
BsuRI GGCC 9 cut(s) 357, 553, 608, 835, 1120, 1394, 1464, 2352, 2751
BtgI CCRYGG 1 cut(s) 545
BtrI CACGTC 1 cut(s) 969
BtsI GCAGTG 3 cut(s) 1591, 2035, 2188
BtsIMutI CAGTG 4 cut(s) 1591, 1689, 2035, 2188
BveI ACCTGC 2 cut(s) 2148, 2335
Cac8I GCNNGC 7 cut(s) 555, 750, 921, 1421, 2217, 2268, 2478
CaiI CAGNNNCTG 4 cut(s) 1073, 2243, 2429, 2713
CfoI GCGC 3 cut(s) 1063, 1584, 2456
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 6 cut(s) 126, 356, 387, 1118, 1392, 2351
CseI GACGC 3 cut(s) 25, 587, 2673
CsiI ACCWGGT 1 cut(s) 2230
Csp6I GTAC 2 cut(s) 1521, 1660
CviQI GTAC 2 cut(s) 1521, 1660
EaeI YGGCCR 3 cut(s) 833, 1462, 2749
EagI CGGCCG 1 cut(s) 833
Eam1104I CTCTTC 7 cut(s) 405, 992, 1226, 1871, 2069, 2159, 2171
EarI CTCTTC 7 cut(s) 405, 992, 1226, 1871, 2069, 2159, 2171
EciI GGCGGA 1 cut(s) 1339
EclXI CGGCCG 1 cut(s) 833
Eco130I CCWWGG 1 cut(s) 1114
Eco31I GGTCTC 2 cut(s) 374, 1541
Eco32I GATATC 1 cut(s) 1833
Eco47I GGWCC 2 cut(s) 126, 387
Eco52I CGGCCG 1 cut(s) 833
Eco57I CTGAAG 2 cut(s) 965, 1536
Eco88I CYCGRG 2 cut(s) 118, 1843
EcoNI CCTNNNNNAGG 4 cut(s) 38, 821, 1495, 2470
EcoRII CCWGG 9 cut(s) 33, 621, 1072, 2098, 2230, 2242, 2353, 2624, 2643
EcoRV GATATC 1 cut(s) 1833
EcoT14I CCWWGG 1 cut(s) 1114
ErhI CCWWGG 1 cut(s) 1114
Esp3I CGTCTC 1 cut(s) 1778
FaqI GGGAC 8 cut(s) 28, 379, 400, 448, 507, 653, 1091, 1651
FauI CCCGC 3 cut(s) 1116, 2620, 2674
FbaI TGATCA 4 cut(s) 292, 1165, 1621, 1885
FblI GTMKAC 1 cut(s) 2204
Fnu4HI GCNGC 8 cut(s) 671, 746, 1064, 1941, 2565, 2568, 2670, 2673
Fsp4HI GCNGC 8 cut(s) 671, 746, 1064, 1941, 2565, 2568, 2670, 2673
FspBI CTAG 4 cut(s) 972, 1458, 1706, 2477
FspI TGCGCA 1 cut(s) 1583
GlaI GCGC 3 cut(s) 1062, 1583, 2455
GluI GCNGC 8 cut(s) 671, 746, 1064, 1941, 2565, 2568, 2670, 2673
GsuI CTGGAG 5 cut(s) 56, 459, 1947, 2735, 2801
HaeIII GGCC 9 cut(s) 357, 553, 608, 835, 1120, 1394, 1464, 2352, 2751
HgaI GACGC 3 cut(s) 25, 587, 2673
HhaI GCGC 3 cut(s) 1063, 1584, 2456
Hin1I GRCGYC 1 cut(s) 2684
Hin6I GCGC 3 cut(s) 1061, 1582, 2454
HinP1I GCGC 3 cut(s) 1061, 1582, 2454
HindIII AAGCTT 2 cut(s) 896, 1397
HphI GGTGA 8 cut(s) 217, 283, 311, 524, 1367, 1577, 2316, 2690
Hpy166II GTNNAC 5 cut(s) 397, 966, 1595, 2205, 2585
Hpy8I GTNNAC 5 cut(s) 397, 966, 1595, 2205, 2585
Hpy99I CGWCG 2 cut(s) 1058, 2689
HpyCH4III ACNGT 2 cut(s) 681, 2519
HpyCH4IV ACGT 4 cut(s) 968, 1787, 1917, 2538
HpySE526I ACGT 4 cut(s) 968, 1787, 1917, 2538
Hsp92I GRCGYC 1 cut(s) 2684
HspAI GCGC 3 cut(s) 1061, 1582, 2454
Kpn2I TCCGGA 2 cut(s) 82, 2281
KroI GCCGGC 1 cut(s) 919
KroNI GCCGGC 1 cut(s) 921
Ksp22I TGATCA 4 cut(s) 292, 1165, 1621, 1885
LguI GCTCTTC 3 cut(s) 1226, 1871, 2171
LmnI GCTCC 8 cut(s) 420, 709, 1116, 1289, 1500, 1653, 2528, 2614
Lsp1109I GCAGC 8 cut(s) 682, 757, 1075, 1952, 2554, 2576, 2656, 2659
LweI GCATC 4 cut(s) 178, 1755, 2339, 2473
MabI ACCWGGT 1 cut(s) 2230
MaeI CTAG 4 cut(s) 972, 1458, 1706, 2477
MaeII ACGT 4 cut(s) 968, 1787, 1917, 2538
MaeIII GTNAC 6 cut(s) 109, 254, 1788, 1913, 2341, 2764
MbiI CCGCTC 2 cut(s) 1111, 1935
MflI RGATCY 2 cut(s) 2033, 2357
MhlI GDGCHC 1 cut(s) 1421
MlsI TGGCCA 1 cut(s) 2751
MluNI TGGCCA 1 cut(s) 2751
MlyI GAGTC 7 cut(s) 110, 251, 612, 1711, 1796, 1938, 2009
MmeI TCCRAC 6 cut(s) 219, 298, 687, 745, 2559, 2739
Mox20I TGGCCA 1 cut(s) 2751
MroI TCCGGA 2 cut(s) 82, 2281
MroNI GCCGGC 1 cut(s) 919
MroXI GAANNNNTTC 1 cut(s) 867
MscI TGGCCA 1 cut(s) 2751
MseI TTAA 7 cut(s) 534, 735, 858, 1493, 1947, 1989, 2699
MslI CAYNNNNRTG 1 cut(s) 1424
Msp20I TGGCCA 1 cut(s) 2751
MspA1I CMGCKG 5 cut(s) 673, 985, 1943, 2567, 2669
Mva1269I GAATGC 1 cut(s) 994
MvaI CCWGG 9 cut(s) 35, 623, 1074, 2100, 2232, 2244, 2355, 2626, 2645
MvnI CGCG 1 cut(s) 19
NaeI GCCGGC 1 cut(s) 921
NciI CCSGG 3 cut(s) 359, 385, 449
NgoMIV GCCGGC 1 cut(s) 919
NheI GCTAGC 1 cut(s) 2476
NlaIV GGNNCC 7 cut(s) 127, 167, 388, 1489, 2035, 2359, 2664
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 3 cut(s) 109, 2341, 2764
NsbI TGCGCA 1 cut(s) 1583
NspI RCATGY 2 cut(s) 950, 2270
NspV TTCGAA 2 cut(s) 1850, 2573
PaeI GCATGC 1 cut(s) 2270
PaeR7I CTCGAG 1 cut(s) 1843
PaqCI CACCTGC 1 cut(s) 2335
PciI ACATGT 1 cut(s) 946
PciSI GCTCTTC 3 cut(s) 1226, 1871, 2171
PcsI WCGNNNNNNNCGW 1 cut(s) 2765
PctI GAATGC 1 cut(s) 994
PdiI GCCGGC 1 cut(s) 921
PdmI GAANNNNTTC 1 cut(s) 867
PfeI GAWTC 8 cut(s) 1348, 1376, 1721, 1771, 2090, 2153, 2169, 2741
PflFI GACNNNGTC 1 cut(s) 2191
PflMI CCANNNNNTGG 1 cut(s) 1073
PkrI GCNGC 8 cut(s) 672, 747, 1065, 1942, 2566, 2569, 2671, 2674
Ple19I CGATCG 1 cut(s) 99
PleI GAGTC 7 cut(s) 110, 250, 612, 1710, 1796, 1937, 2009
PpsI GAGTC 7 cut(s) 110, 250, 612, 1710, 1796, 1937, 2009
PscI ACATGT 1 cut(s) 946
PsiI TTATAA 1 cut(s) 1737
Psp6I CCWGG 9 cut(s) 33, 621, 1072, 2098, 2230, 2242, 2353, 2624, 2643
PspGI CCWGG 9 cut(s) 33, 621, 1072, 2098, 2230, 2242, 2353, 2624, 2643
PspN4I GGNNCC 7 cut(s) 127, 167, 388, 1489, 2035, 2359, 2664
PspPI GGNCC 6 cut(s) 126, 356, 387, 1118, 1392, 2351
PspXI VCTCGAGB 1 cut(s) 1843
PstI CTGCAG 2 cut(s) 1942, 2134
PstNI CAGNNNCTG 4 cut(s) 1073, 2243, 2429, 2713
PsuI RGATCY 2 cut(s) 2033, 2357
PsyI GACNNNGTC 1 cut(s) 2191
PvuI CGATCG 1 cut(s) 99
PvuII CAGCTG 4 cut(s) 673, 985, 1943, 2567
RsaI GTAC 2 cut(s) 1522, 1661
RsaNI GTAC 2 cut(s) 1521, 1660
RseI CAYNNNNRTG 1 cut(s) 1424
SapI GCTCTTC 3 cut(s) 1226, 1871, 2171
SaqAI TTAA 7 cut(s) 534, 735, 858, 1493, 1947, 1989, 2699
SatI GCNGC 8 cut(s) 671, 746, 1064, 1941, 2565, 2568, 2670, 2673
Sau96I GGNCC 6 cut(s) 126, 356, 387, 1118, 1392, 2351
ScaI AGTACT 1 cut(s) 1661
SchI GAGTC 7 cut(s) 110, 251, 612, 1711, 1796, 1938, 2009
SduI GDGCHC 1 cut(s) 1421
SexAI ACCWGGT 1 cut(s) 2230
SfaNI GCATC 4 cut(s) 178, 1755, 2339, 2473
SfcI CTRYAG 3 cut(s) 1806, 1938, 2130
Sfr274I CTCGAG 1 cut(s) 1843
SfuI TTCGAA 2 cut(s) 1850, 2573
SinI GGWCC 2 cut(s) 126, 387
SlaI CTCGAG 1 cut(s) 1843
SmiMI CAYNNNNRTG 1 cut(s) 1424
SmlI CTYRAG 3 cut(s) 1079, 1191, 1843
SmoI CTYRAG 3 cut(s) 1079, 1191, 1843
SphI GCATGC 1 cut(s) 2270
SspMI CTAG 4 cut(s) 972, 1458, 1706, 2477
StyI CCWWGG 1 cut(s) 1114
TaaI ACNGT 2 cut(s) 681, 2519
TaiI ACGT 4 cut(s) 971, 1790, 1920, 2541
TaqI TCGA 9 cut(s) 251, 722, 1056, 1844, 1850, 2018, 2573, 2694, 2774
TaqII GACCGA 3 cut(s) 128, 404, 2133
TatI WGTACW 1 cut(s) 1659
TfiI GAWTC 8 cut(s) 1348, 1376, 1721, 1771, 2090, 2153, 2169, 2741
Tru1I TTAA 7 cut(s) 534, 735, 858, 1493, 1947, 1989, 2699
Tru9I TTAA 7 cut(s) 534, 735, 858, 1493, 1947, 1989, 2699
TscAI CASTG 4 cut(s) 1591, 1696, 2035, 2188
TseFI GTSAC 3 cut(s) 109, 2341, 2764
TseI GCWGC 8 cut(s) 670, 745, 1063, 1940, 2564, 2567, 2669, 2672
Tsp45I GTSAC 3 cut(s) 109, 2341, 2764
TspDTI ATGAA 8 cut(s) 90, 654, 1194, 1442, 1622, 1666, 1907, 2543
TspGWI ACGGA 6 cut(s) 321, 414, 534, 657, 1993, 2511
TspRI CASTG 4 cut(s) 1591, 1696, 2035, 2188
Tth111I GACNNNGTC 1 cut(s) 2191
Van91I CCANNNNNTGG 1 cut(s) 1073
VpaK11BI GGWCC 2 cut(s) 126, 387
XagI CCTNNNNNAGG 4 cut(s) 38, 821, 1495, 2470
XapI RAATTY 2 cut(s) 561, 1183
XbaI TCTAGA 1 cut(s) 1705
XceI RCATGY 2 cut(s) 950, 2270
XcmI CCANNNNNNNNNTGG 1 cut(s) 1128
XhoI CTCGAG 1 cut(s) 1843
XmiI GTMKAC 1 cut(s) 2204
XmnI GAANNNNTTC 1 cut(s) 867
XspI CTAG 4 cut(s) 972, 1458, 1706, 2477
ZrmI AGTACT 1 cut(s) 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.