Rh2BG646900

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002210
Physical Location & Seq
Reverse (-)
86613403 .. 86617516
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG646900.1

Sequence Viewer

Length: 3258 bp
ATGGGGACGTTGGGGAGATCTACAGCCGTAGCTGATGGTTGCTCGGGTGGCTCCGGGTCGGGTTCTGGACCGGCGAGGTTCGAGGCCGGGGAGGAGAGGAGGAGGATTGACGACTCGGGTGGGAGCCGGGTCGGGTCGGGAGGGTCCGGAGGGGAGCTGGTGAGGTCGTTTGTTGAGCTGGTTAAGACTGTGGAGGCTAAAGTTTCGGTTGGGATGAGTTTTGGGGACATGGATACGTGTATGTCGAGGTTGAGAGGGAGTGAGAGTGTTGGAGAGCTGGAGTCTGGTTTAGGTGAGGAGAGGAGAGTGGTGAGCATTGGTTCGAAGACGAATTCGGATGCGAATGTAGGGGATGAGGACAATGGGAATTGTTGTGATGAAACCAAGACCAACGGTGTTGGGGTTGAGGAGAATGGGAAGTGTTTGGATGAAATTGTGGAAGAGCTAGATGGGGGAATTGAGGTTCCCATTGCCGGTACGAGTGAAAATGGTGAAGACCGGGTGGGAGAAGAGTTGAGTGATGGAGTGAATGAGTTCTGTATCGGAGATTTTGTGTGGGGTAAGATTAAGAACCACCCGTGGTGGCCGGGCCAGATTTGTGATCCTTCGGATGCTTCTGAGTTTGCAGTGAAGTTGAGATCCAAGGACAAACTTCTTGTAGCGTACTTTGGGGATGACACTTTCGCGTGGTGCCATCCGTCGCAATTGAAACCCTTTGAGGAAAATTTCGTGGAGAATTCTAAGCTGAGTAGCTCCAAGGTTTTTGTTATTGCTGTGCAGCAGGCTGTGGATGAGATAGGTAGGCTTGTAAAGTTGAAGATGAGTTGTGCTTGCATGGGGGAAGAGTTTCTAAGTGGTCATAAACAGCCTTTAGCATCGAATGCTGGAGTTAAGGAAGGAGTTGTTGTCCCTGAAGGCCGAGTTGGGAAGTTTTTGGGTCATCTATCTCAGCCGGTTGACCTTCTTGCAGAATTGAAACATATGGCACAAGCTGTATCCTTGACTAGTGTTCTTGAGCTCAATATATTAAAGAGTTTGCTGTCTGCCTTTTATTGTTCGAAAGGGGGATATCAGTTACCTGTTTACATTGAACCCCAGCTAATTCCTGGTCTTGAAGATGAGCGGAAGATGGTGGAAGTCCCAGTGCAGTGGCCATCTGATGACAGGTCGTCTTCTCCCATAGGCTCCAAGACTGGAAACACTGAACAAAATTTGTTGCTAAGGTCACCACCAAGTATAGAAAATAGACAGCCCCATAAAAGGAAGGAGAAGACCATTGCTGATCTTCTTGGAGAAGCCAATCGTGTTGAGGTAAAAAGTATGGATGGGGTTGTAGCTAAAGAAAGTGCAATCTCAGAGAAACGAGGGCTATCATCCAGACGGAAGAAGCAGAAAAGCAGTGATATTCATGGTGAGGGTAGATTGACTTTCGAATCAGGAAGGGAAAGAGCCAAACTCTCAAAATCGCCCACAAGCACTTTAGCGAGGAGAAAGGAAGAAGATGAAGGTATTGGCATCGAGAGTAGTGATGGTTTGGCTAAAGAGAGTGCTGGTGGAAATGAAGAAACTAAGAAGACTCCTCTCTCAAGGAAAAGGAAGAGGGATGAAGATAGCATCGACAGTAAAGATGGTGATACCCAAGAAAAAAGTGGTGGAAAGGATGAAACTAAGAAGGCTCCGTTGTCAAGGAGTAGGAAGAAGAATGAAGATATTCACATCAACAGTAATGATGGTGAGGCCAAAGGAAAAGCTGGTGCAAGAGTCGGGGAGTTGCTCAATGTTGGTTTACAAACAGATATGAAAGACCAAATTGGTAAGAGCCCTTTGTTGAGGGAAAGAAAAAAAAGCAAGTACTTGTCCCCACCCTTCACAATAAGTGACGCAGGGAAAAGAAAACGAAACCTGGAAACAGAATCTCGAAATGCTGTAACGAGTCATGATTTGGGGAAAGAGGGGAAAGAGCAAGACATGGAATCAGTATTATCTCTGGAGGTTTCTAGTAAAGCTCAGCTTGGGGACAGGATTACCAGGGCTGCTGCCAACATTAAGGGGTCCCCTCAGATTTTGAATAGTTGCAGTGAGGCATTACAAGAGAAACTCTCAATAGAAGGTGGGAATAAGAGCACTGATGTTAAAACACCAGCAGAAGGTGAGAAGAAGCGCGTTGATCCAATAAAAGCTAATGCAGCCACTAATGAAGTGCTATCTGGAGTCCGCTCTGCAGCTCTTGATCCATTATATCCAGTCAAAAGGAAATCTTCCTTTGAGATTGTTGTTGATTTTGTGTCCATATTTAGAGACTCAGTTTACCACAATGGCTGCTTCCACAAGCTTTACAAGAAGCACCGACCTCATGGAAAGAGGAAGAGATCAGATTCTGAACTGGGGTCATTGGTGAAAGACAATCGAACTGCTGCAAATTTGCATGAAGATGAACCTGGTAACAGGGTTATTAAGAAGAAACAAGATATGAAGTCTGATAAGCCTACAAAAAAGCAAGCTGGTGAGACACGAAGTTCAAAGAGAAGGAAGTTAAATTCTGTAATTAAGTCGTTGGGAAAAGACAATGAGAAAGCAGAAAATCAGCCTGTCAGTGAATCTGGGGAGAGGAACATCAACACTAACCAAGATAAGCCTAAGAAAAAGCAAACTGCTGAGCCACCAATTTCAAAGCTGGTCAGGCGGAGATCAAAGCATGCTGACACTCGGGATTTGAAGATTAAGGAGACGGAAGAGGAGGCTCCGCCTACATTCCTTTTTGTAACATTTGGACCTGGATCCTCGCTGCCTACCAAGGATGATCTTCTCAGATTATACAGTAAATTTGGGGAACTAAATGAAACTGAAACTGAGATGTTCTACACCAATTTCTGTGCTCGTGTTGCGTTTGTAATGAGCTCTGACGCGGAAGAAGCCTTCAATCATTCTCTAATTGACAGTCCCTTTGGAGCTTCCAATGTCAATTTCCGCCTCCAAAATCCCTCAGGTGCTTCCAAGACTCGTGAGCTCAGTGCAATACCCGAGTCTCCTCCAGCTAAGAAGTCCAGGGGCAAGACTGCAAGTGAGGCATTAGTAGCTTCACAACCACCTGCTGGTGAGCCATCGCAAGTTGATTTCATTAGGCATAGACTTAAGATGATGAGCTCAATGCTGGGAAACTCAGATCGCAAAGTGTCGCCTGTTGCAACATCGAAAATTCAGAATGAGATAAAGGAGCTACTGAAGGTCGTAAGCACAATGGGTGAATCCTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1085

Amino Acids

118.28

Weight (kDa)

8.44

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 182 - 265 3.1e-20 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3097
Acc36I ACCTGC 1 cut(s) 3097
AccB1I GGYRCC 1 cut(s) 690
AccB7I CCANNNNNTGG 1 cut(s) 3092
AccBSI CCGCTC 2 cut(s) 1123, 2217
AccII CGCG 3 cut(s) 686, 2163, 2906
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 6 cut(s) 1123, 2215, 2683, 2744, 2906, 2968
AclWI GGATC 6 cut(s) 596, 633, 2162, 2225, 2772, 2785
AcoI YGGCCR 2 cut(s) 584, 1151
AcsI RAATTY 8 cut(s) 331, 724, 736, 1210, 2419, 2536, 2822, 3195
AcuI CTGAAG 2 cut(s) 933, 3242
AfaI GTAC 3 cut(s) 478, 665, 1853
AfiI CCNNNNNNNGG 4 cut(s) 473, 1260, 2147, 3092
AflII CTTAAG 1 cut(s) 3131
AflIII ACRYGT 1 cut(s) 236
AhdI GACNNNNNGTC 1 cut(s) 1168
AhlI ACTAGT 1 cut(s) 1004
AjnI CCWGG 6 cut(s) 1105, 1902, 2027, 2437, 2773, 3044
AjuI GAANNNNNNNTTGG 4 cut(s) 906, 938, 2948, 2980
AloI GAACNNNNNNTCC 2 cut(s) 447, 479
Alw21I GWGCWC 6 cut(s) 1020, 2126, 2878, 2900, 3009, 3146
Alw26I GTCTC 4 cut(s) 2292, 2501, 2720, 3030
AlwI GGATC 6 cut(s) 596, 633, 2162, 2225, 2772, 2785
AlwNI CAGNNNCTG 1 cut(s) 2378
Ama87I CYCGRG 4 cut(s) 43, 115, 2706, 3020
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 6 cut(s) 84, 584, 589, 916, 1151, 1737
ApeKI GCWGC 8 cut(s) 778, 2033, 2036, 2186, 2222, 2319, 2414, 2785
ApoI RAATTY 8 cut(s) 331, 724, 736, 1210, 2419, 2536, 2822, 3195
ArsI GACNNNNNNTTYG 2 cut(s) 2927, 2959
Asp700I GAANNNNTTC 3 cut(s) 533, 846, 1710
AspLEI GCGC 1 cut(s) 2163
AspS9I GGNCC 5 cut(s) 68, 144, 589, 2052, 2771
AsuC2I CCSGG 5 cut(s) 55, 88, 128, 500, 588
AsuII TTCGAA 3 cut(s) 323, 1058, 1431
AvaI CYCGRG 4 cut(s) 43, 115, 2706, 3020
AvaII GGWCC 4 cut(s) 68, 144, 2052, 2771
AxyI CCTNAGG 1 cut(s) 2983
BalI TGGCCA 1 cut(s) 1153
BamHI GGATCC 1 cut(s) 2777
BanI GGYRCC 1 cut(s) 690
BanII GRGCYC 5 cut(s) 1020, 1823, 2900, 3009, 3146
BauI CACGAG 2 cut(s) 2877, 3000
BbsI GAAGAC 5 cut(s) 332, 501, 1164, 1277, 1580
Bbv12I GWGCWC 6 cut(s) 1020, 2126, 2878, 2900, 3009, 3146
BbvI GCAGC 8 cut(s) 790, 2020, 2023, 2198, 2234, 2306, 2401, 2772
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 6 cut(s) 1107, 1904, 2029, 2439, 2775, 3046
BciVI GTATCC 2 cut(s) 226, 1006
BcnI CCSGG 5 cut(s) 55, 88, 128, 500, 588
BcoDI GTCTC 4 cut(s) 2292, 2501, 2720, 3030
BcuI ACTAGT 1 cut(s) 1004
BfaI CTAG 3 cut(s) 446, 1005, 1998
BfmI CTRYAG 2 cut(s) 21, 2220
BfrI CTTAAG 1 cut(s) 3131
BfuAI ACCTGC 1 cut(s) 3097
BfuI GTATCC 2 cut(s) 226, 1006
BglII AGATCT 1 cut(s) 17
BisI GCNGC 8 cut(s) 779, 2034, 2037, 2187, 2223, 2320, 2415, 2786
BlpI GCTNAGC 2 cut(s) 2007, 2655
BlsI GCNGC 8 cut(s) 780, 2035, 2038, 2188, 2224, 2321, 2416, 2787
BmcAI AGTACT 1 cut(s) 1853
Bme18I GGWCC 4 cut(s) 68, 144, 2052, 2771
BmeRI GACNNNNNGTC 1 cut(s) 1168
BmeT110I CYCGRG 4 cut(s) 43, 115, 2706, 3020
BmgT120I GGNCC 5 cut(s) 68, 144, 589, 2052, 2771
BmrI ACTGGG 2 cut(s) 1136, 2393
BmsI GCATC 5 cut(s) 328, 601, 884, 1524, 1623
BmuI ACTGGG 2 cut(s) 1136, 2393
BpiI GAAGAC 5 cut(s) 332, 501, 1164, 1277, 1580
BplI GAGNNNNNCTC 4 cut(s) 1440, 1472, 2084, 2116
BpmI CTGGAG 5 cut(s) 299, 906, 2009, 2229, 3015
Bpu10I CCTNAGC 1 cut(s) 1220
Bpu1102I GCTNAGC 2 cut(s) 2007, 2655
Bpu14I TTCGAA 3 cut(s) 323, 1058, 1431
BpuEI CTTGAG 2 cut(s) 1034, 1570
BpuMI CCSGG 5 cut(s) 55, 88, 128, 500, 588
BsaAI YACGTR 1 cut(s) 237
BsaJI CCNNGG 7 cut(s) 87, 578, 642, 756, 2028, 2793, 3045
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 6 cut(s) 250, 280, 537, 567, 891, 921
Bsc4I CCNNNNNNNGG 4 cut(s) 473, 1260, 2147, 3092
Bse118I RCCGGY 3 cut(s) 70, 473, 952
Bse1I ACTGG 4 cut(s) 1142, 1198, 2243, 2388
Bse21I CCTNAGG 1 cut(s) 2983
Bse3DI GCAATG 2 cut(s) 468, 1275
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 6 cut(s) 1107, 1904, 2029, 2439, 2775, 3046
BseDI CCNNGG 7 cut(s) 87, 578, 642, 756, 2028, 2793, 3045
BseLI CCNNNNNNNGG 4 cut(s) 473, 1260, 2147, 3092
BseMI GCAATG 2 cut(s) 468, 1275
BseNI ACTGG 4 cut(s) 1142, 1198, 2243, 2388
BseXI GCAGC 8 cut(s) 790, 2020, 2023, 2198, 2234, 2306, 2401, 2772
BseYI CCCAGC 2 cut(s) 1095, 3151
BsgI GTGCAG 2 cut(s) 797, 1166
Bsh1236I CGCG 3 cut(s) 686, 2163, 2906
BshFI GGCC 6 cut(s) 86, 586, 591, 918, 1153, 1739
BshNI GGYRCC 1 cut(s) 690
BsiHKAI GWGCWC 6 cut(s) 1020, 2126, 2878, 2900, 3009, 3146
BsiHKCI CYCGRG 4 cut(s) 43, 115, 2706, 3020
BsiSI CCGG 9 cut(s) 54, 71, 87, 127, 147, 474, 499, 587, 953
BslFI GGGAC 8 cut(s) 19, 239, 893, 1124, 1843, 2030, 2038, 2925
BslI CCNNNNNNNGG 4 cut(s) 473, 1260, 2147, 3092
BsmAI GTCTC 4 cut(s) 2292, 2501, 2720, 3030
BsmBI CGTCTC 1 cut(s) 2720
BsmFI GGGAC 8 cut(s) 19, 239, 893, 1124, 1843, 2030, 2038, 2925
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 6 cut(s) 86, 586, 591, 918, 1153, 1739
BsoBI CYCGRG 4 cut(s) 43, 115, 2706, 3020
Bsp119I TTCGAA 3 cut(s) 323, 1058, 1431
Bsp1286I GDGCHC 7 cut(s) 1020, 1823, 2126, 2878, 2900, 3009, 3146
Bsp13I TCCGGA 1 cut(s) 146
Bsp1720I GCTNAGC 2 cut(s) 2007, 2655
BspACI CCGC 6 cut(s) 1123, 2215, 2683, 2744, 2906, 2968
BspANI GGCC 6 cut(s) 86, 586, 591, 918, 1153, 1739
BspEI TCCGGA 1 cut(s) 146
BspFNI CGCG 3 cut(s) 686, 2163, 2906
BspHI TCATGA 1 cut(s) 1936
BspMAI CTGCAG 1 cut(s) 2224
BspMI ACCTGC 1 cut(s) 3097
BspPI GGATC 6 cut(s) 596, 633, 2162, 2225, 2772, 2785
BspQI GCTCTTC 1 cut(s) 435
BspT104I TTCGAA 3 cut(s) 323, 1058, 1431
BspT107I GGYRCC 1 cut(s) 690
BspTI CTTAAG 1 cut(s) 3131
BsrBI CCGCTC 2 cut(s) 1123, 2217
BsrDI GCAATG 2 cut(s) 468, 1275
BsrFI RCCGGY 3 cut(s) 70, 473, 952
BsrI ACTGG 4 cut(s) 1142, 1198, 2243, 2388
BssAI RCCGGY 3 cut(s) 70, 473, 952
BssECI CCNNGG 7 cut(s) 87, 578, 642, 756, 2028, 2793, 3045
BssSI CACGAG 2 cut(s) 2877, 3000
BssT1I CCWWGG 3 cut(s) 642, 756, 2793
Bst2BI CACGAG 2 cut(s) 2877, 3000
Bst2UI CCWGG 6 cut(s) 1107, 1904, 2029, 2439, 2775, 3046
Bst4CI ACNGT 6 cut(s) 190, 395, 1622, 1724, 2819, 2939
Bst6I CTCTTC 6 cut(s) 435, 504, 837, 1592, 2360, 2727
BstAFI CTTAAG 1 cut(s) 3131
BstAPI GCANNNNNTGC 1 cut(s) 881
BstBAI YACGTR 1 cut(s) 237
BstBI TTCGAA 3 cut(s) 323, 1058, 1431
BstC8I GCNNGC 4 cut(s) 783, 832, 2499, 2697
BstDSI CCRYGG 1 cut(s) 578
BstEII GGTNACC 1 cut(s) 1224
BstFNI CGCG 3 cut(s) 686, 2163, 2906
BstHHI GCGC 1 cut(s) 2163
BstMAI GTCTC 4 cut(s) 2292, 2501, 2720, 3030
BstMWI GCNNNNNNNGC 8 cut(s) 48, 881, 2186, 2680, 2882, 2912, 3065, 3074
BstNI CCWGG 6 cut(s) 1107, 1904, 2029, 2439, 2775, 3046
BstNSI RCATGY 1 cut(s) 2699
BstPI GGTNACC 1 cut(s) 1224
BstSFI CTRYAG 2 cut(s) 21, 2220
BstUI CGCG 3 cut(s) 686, 2163, 2906
BstV1I GCAGC 8 cut(s) 790, 2020, 2023, 2198, 2234, 2306, 2401, 2772
BstV2I GAAGAC 5 cut(s) 332, 501, 1164, 1277, 1580
BstX2I RGATCY 3 cut(s) 17, 638, 2777
BstXI CCANNNNNNTGG 1 cut(s) 1149
BstYI RGATCY 3 cut(s) 17, 638, 2777
Bsu36I CCTNAGG 1 cut(s) 2983
BsuI GTATCC 2 cut(s) 226, 1006
BsuRI GGCC 6 cut(s) 86, 586, 591, 918, 1153, 1739
BtgI CCRYGG 1 cut(s) 578
BtgZI GCGATG 1 cut(s) 3087
BtsI GCAGTG 4 cut(s) 633, 1154, 1405, 2083
BtsIMutI CAGTG 9 cut(s) 633, 1149, 1154, 1200, 1405, 2083, 2124, 2599, 3016
BveI ACCTGC 1 cut(s) 3097
Cac8I GCNNGC 4 cut(s) 783, 832, 2499, 2697
CaiI CAGNNNCTG 1 cut(s) 2378
CciI TCATGA 1 cut(s) 1936
CfoI GCGC 1 cut(s) 2163
Cfr10I RCCGGY 3 cut(s) 70, 473, 952
Cfr13I GGNCC 5 cut(s) 68, 144, 589, 2052, 2771
CseI GACGC 2 cut(s) 1889, 2912
CsiI ACCWGGT 1 cut(s) 2437
Csp6I GTAC 3 cut(s) 477, 664, 1852
CviAII CATG 8 cut(s) 229, 835, 1409, 1937, 1969, 2354, 2426, 2696
CviQI GTAC 3 cut(s) 477, 664, 1852
DriI GACNNNNNGTC 1 cut(s) 1168
EaeI YGGCCR 2 cut(s) 584, 1151
Eam1104I CTCTTC 6 cut(s) 435, 504, 837, 1592, 2360, 2727
Eam1105I GACNNNNNGTC 1 cut(s) 1168
EarI CTCTTC 6 cut(s) 435, 504, 837, 1592, 2360, 2727
EciI GGCGGA 3 cut(s) 2698, 2733, 2957
Ecl136II GAGCTC 4 cut(s) 1018, 2898, 3007, 3144
Eco130I CCWWGG 3 cut(s) 642, 756, 2793
Eco24I GRGCYC 5 cut(s) 1020, 1823, 2900, 3009, 3146
Eco32I GATATC 1 cut(s) 1070
Eco47I GGWCC 4 cut(s) 68, 144, 2052, 2771
Eco53kI GAGCTC 4 cut(s) 1018, 2898, 3007, 3144
Eco57I CTGAAG 2 cut(s) 933, 3242
Eco81I CCTNAGG 1 cut(s) 2983
Eco88I CYCGRG 4 cut(s) 43, 115, 2706, 3020
Eco91I GGTNACC 1 cut(s) 1224
EcoICRI GAGCTC 4 cut(s) 1018, 2898, 3007, 3144
EcoO109I RGGNCCY 1 cut(s) 2052
EcoO65I GGTNACC 1 cut(s) 1224
EcoRI GAATTC 2 cut(s) 331, 736
EcoRII CCWGG 6 cut(s) 1105, 1902, 2027, 2437, 2773, 3044
EcoRV GATATC 1 cut(s) 1070
EcoT14I CCWWGG 3 cut(s) 642, 756, 2793
EcoT38I GRGCYC 5 cut(s) 1020, 1823, 2900, 3009, 3146
ErhI CCWWGG 3 cut(s) 642, 756, 2793
Esp3I CGTCTC 1 cut(s) 2720
FaeI CATG 8 cut(s) 232, 838, 1412, 1940, 1972, 2357, 2429, 2699
FalI AAGNNNNNCTT 4 cut(s) 1995, 2027, 2242, 2274
FaqI GGGAC 8 cut(s) 19, 239, 893, 1124, 1843, 2030, 2038, 2925
FatI CATG 8 cut(s) 228, 834, 1408, 1936, 1968, 2353, 2425, 2695
FauNDI CATATG 1 cut(s) 981
Fnu4HI GCNGC 8 cut(s) 779, 2034, 2037, 2187, 2223, 2320, 2415, 2786
FriOI GRGCYC 5 cut(s) 1020, 1823, 2900, 3009, 3146
Fsp4HI GCNGC 8 cut(s) 779, 2034, 2037, 2187, 2223, 2320, 2415, 2786
FspBI CTAG 3 cut(s) 446, 1005, 1998
GlaI GCGC 1 cut(s) 2162
GluI GCNGC 8 cut(s) 779, 2034, 2037, 2187, 2223, 2320, 2415, 2786
GsaI CCCAGC 2 cut(s) 1099, 3155
GsuI CTGGAG 5 cut(s) 299, 906, 2009, 2229, 3015
HaeIII GGCC 6 cut(s) 86, 586, 591, 918, 1153, 1739
HapII CCGG 9 cut(s) 54, 71, 87, 127, 147, 474, 499, 587, 953
HgaI GACGC 2 cut(s) 1889, 2912
HhaI GCGC 1 cut(s) 2163
Hin1II CATG 8 cut(s) 232, 838, 1412, 1940, 1972, 2357, 2429, 2699
Hin6I GCGC 1 cut(s) 2161
HinP1I GCGC 1 cut(s) 2161
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HindIII AAGCTT 1 cut(s) 2330
HpaII CCGG 9 cut(s) 54, 71, 87, 127, 147, 474, 499, 587, 953
Hpy166II GTNNAC 4 cut(s) 958, 1084, 1787, 2308
Hpy8I GTNNAC 4 cut(s) 958, 1084, 1787, 2308
Hpy99I CGWCG 1 cut(s) 703
HpyCH4III ACNGT 6 cut(s) 190, 395, 1622, 1724, 2819, 2939
HpyCH4IV ACGT 2 cut(s) 8, 236
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 881, 2186, 2680, 2882, 2912, 3065, 3074
HpySE526I ACGT 2 cut(s) 8, 236
Hsp92II CATG 8 cut(s) 232, 838, 1412, 1940, 1972, 2357, 2429, 2699
HspAI GCGC 1 cut(s) 2161
KflI GGGWCCC 1 cut(s) 2052
Kpn2I TCCGGA 1 cut(s) 146
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 9 cut(s) 56, 123, 154, 758, 1190, 1681, 2746, 2948, 3214
Lsp1109I GCAGC 8 cut(s) 790, 2020, 2023, 2198, 2234, 2306, 2401, 2772
LweI GCATC 5 cut(s) 328, 601, 884, 1524, 1623
MabI ACCWGGT 1 cut(s) 2437
MaeI CTAG 3 cut(s) 446, 1005, 1998
MaeII ACGT 2 cut(s) 8, 236
MaeIII GTNAC 6 cut(s) 1074, 1224, 1877, 1927, 2441, 2761
MbiI CCGCTC 2 cut(s) 1123, 2217
MfeI CAATTG 1 cut(s) 704
MflI RGATCY 3 cut(s) 17, 638, 2777
MhlI GDGCHC 7 cut(s) 1020, 1823, 2126, 2878, 2900, 3009, 3146
MlsI TGGCCA 1 cut(s) 1153
MluNI TGGCCA 1 cut(s) 1153
MlyI GAGTC 9 cut(s) 107, 290, 1570, 1770, 1942, 2220, 2294, 2992, 3032
MmeI TCCRAC 1 cut(s) 250
Mox20I TGGCCA 1 cut(s) 1153
MroI TCCGGA 1 cut(s) 146
MroXI GAANNNNTTC 3 cut(s) 533, 846, 1710
MscI TGGCCA 1 cut(s) 1153
MslI CAYNNNNRTG 1 cut(s) 2430
Msp20I TGGCCA 1 cut(s) 1153
MspCI CTTAAG 1 cut(s) 3131
MspI CCGG 9 cut(s) 54, 71, 87, 127, 147, 474, 499, 587, 953
MunI CAATTG 1 cut(s) 704
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 6 cut(s) 1107, 1904, 2029, 2439, 2775, 3046
MvnI CGCG 3 cut(s) 686, 2163, 2906
MwoI GCNNNNNNNGC 8 cut(s) 48, 881, 2186, 2680, 2882, 2912, 3065, 3074
NciI CCSGG 5 cut(s) 55, 88, 128, 500, 588
NdeI CATATG 1 cut(s) 981
NlaIII CATG 8 cut(s) 232, 838, 1412, 1940, 1972, 2357, 2429, 2699
NmeAIII GCCGAG 1 cut(s) 944
NmuCI GTSAC 2 cut(s) 1224, 1877
NspI RCATGY 1 cut(s) 2699
NspV TTCGAA 3 cut(s) 323, 1058, 1431
PaeI GCATGC 1 cut(s) 2699
PagI TCATGA 1 cut(s) 1936
PaqCI CACCTGC 1 cut(s) 3097
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PctI GAATGC 1 cut(s) 886
PdmI GAANNNNTTC 3 cut(s) 533, 846, 1710
PfeI GAWTC 6 cut(s) 1433, 1913, 1973, 2375, 2597, 3245
PflMI CCANNNNNTGG 1 cut(s) 3092
PkrI GCNGC 8 cut(s) 780, 2035, 2038, 2188, 2224, 2321, 2416, 2787
PleI GAGTC 9 cut(s) 107, 289, 1570, 1769, 1941, 2219, 2294, 2992, 3031
PpsI GAGTC 9 cut(s) 107, 289, 1570, 1769, 1941, 2219, 2294, 2992, 3031
Ppu21I YACGTR 1 cut(s) 237
PpuMI RGGWCCY 1 cut(s) 2052
Psp124BI GAGCTC 4 cut(s) 1020, 2900, 3009, 3146
Psp5II RGGWCCY 1 cut(s) 2052
Psp6I CCWGG 6 cut(s) 1105, 1902, 2027, 2437, 2773, 3044
PspEI GGTNACC 1 cut(s) 1224
PspFI CCCAGC 2 cut(s) 1095, 3151
PspGI CCWGG 6 cut(s) 1105, 1902, 2027, 2437, 2773, 3044
PspPI GGNCC 5 cut(s) 68, 144, 589, 2052, 2771
PspPPI RGGWCCY 1 cut(s) 2052
PstI CTGCAG 1 cut(s) 2224
PstNI CAGNNNCTG 1 cut(s) 2378
PsuI RGATCY 3 cut(s) 17, 638, 2777
RsaI GTAC 3 cut(s) 478, 665, 1853
RsaNI GTAC 3 cut(s) 477, 664, 1852
RseI CAYNNNNRTG 1 cut(s) 2430
SacI GAGCTC 4 cut(s) 1020, 2900, 3009, 3146
SapI GCTCTTC 1 cut(s) 435
SatI GCNGC 8 cut(s) 779, 2034, 2037, 2187, 2223, 2320, 2415, 2786
Sau96I GGNCC 5 cut(s) 68, 144, 589, 2052, 2771
ScaI AGTACT 1 cut(s) 1853
SchI GAGTC 9 cut(s) 107, 290, 1570, 1770, 1942, 2220, 2294, 2992, 3032
SduI GDGCHC 7 cut(s) 1020, 1823, 2126, 2878, 2900, 3009, 3146
SexAI ACCWGGT 1 cut(s) 2437
SfaNI GCATC 5 cut(s) 328, 601, 884, 1524, 1623
SfcI CTRYAG 2 cut(s) 21, 2220
SfuI TTCGAA 3 cut(s) 323, 1058, 1431
SinI GGWCC 4 cut(s) 68, 144, 2052, 2771
SmiMI CAYNNNNRTG 1 cut(s) 2430
SmlI CTYRAG 3 cut(s) 1013, 1585, 3131
SmoI CTYRAG 3 cut(s) 1013, 1585, 3131
SpeI ACTAGT 1 cut(s) 1004
SphI GCATGC 1 cut(s) 2699
SsiI CCGC 6 cut(s) 1123, 2215, 2683, 2744, 2906, 2968
SspMI CTAG 3 cut(s) 446, 1005, 1998
SstI GAGCTC 4 cut(s) 1020, 2900, 3009, 3146
StyI CCWWGG 3 cut(s) 642, 756, 2793
TaaI ACNGT 6 cut(s) 190, 395, 1622, 1724, 2819, 2939
TaiI ACGT 2 cut(s) 11, 239
TatI WGTACW 1 cut(s) 1851
TfiI GAWTC 6 cut(s) 1433, 1913, 1973, 2375, 2597, 3245
TscAI CASTG 9 cut(s) 633, 1149, 1154, 1207, 1405, 2083, 2131, 2599, 3016
TseFI GTSAC 2 cut(s) 1224, 1877
TseI GCWGC 8 cut(s) 778, 2033, 2036, 2186, 2222, 2319, 2414, 2785
Tsp45I GTSAC 2 cut(s) 1224, 1877
TspGWI ACGGA 4 cut(s) 687, 1396, 1668, 2744
TspRI CASTG 9 cut(s) 633, 1149, 1154, 1207, 1405, 2083, 2131, 2599, 3016
Van91I CCANNNNNTGG 1 cut(s) 3092
Vha464I CTTAAG 1 cut(s) 3131
VpaK11BI GGWCC 4 cut(s) 68, 144, 2052, 2771
XapI RAATTY 8 cut(s) 331, 724, 736, 1210, 2419, 2536, 2822, 3195
XceI RCATGY 1 cut(s) 2699
XcmI CCANNNNNNNNNTGG 2 cut(s) 1103, 1646
XmnI GAANNNNTTC 3 cut(s) 533, 846, 1710
XspI CTAG 3 cut(s) 446, 1005, 1998
ZrmI AGTACT 1 cut(s) 1853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.