RLG00000022100

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002210
Physical Location & Seq
Reverse (-)
80927596 .. 80930868
3273 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022100

Sequence Viewer

Length: 3273 bp
ATGGGGACGTTGGGGAGATCTACAGCCGTAGCTGATGGTTGTTCGGGTGGCTCCGGGTCGGGTTCTGGACCGGCGAGGTTTGAGGCCGGGGAGGAGAGGAGGAGGATTGACGACTCGGGTGGGAGCCGGGTCGGGTCCGGAGGGGAGCTGGTGAGGTCGTTTGTTGAGCTGGTTAAGACTGTGGAGGCTAAAGTTTCGGTTGGGATGAGTTTTGGGGACATGGATACGTGTATGTCGAGGTTGAGAGGGAGTGAGAGTGTTGGAGAGCTGGAGTCTGGTTTAGGTGAGGAGAGGAGAGTGGTGAGCATTGGTTCGAAGACGAATTCGGATGCGAATGTAGGGGGGGATGAGGACAATGGGAATTGTTGTGATGAAAACAAGAGCAACGGTGGTGGGAACGACGGGGTTGAGGAGAATGGGAAGTGTTTGGATGAAATTGGGGAAGAGCTAGATGGGGGAATTGAGGTTCCCATTGCCGGTACGAGTGAAAATGGTGAAGACTGGGTGAGGGAAGAGTTGAGTGATGGAGTGAATGAGTTCTGTATCGGAGATTTTGTGTGGGGTAAGATTAAGAACCACCCGTGGTGGCCGGGCCAGATTTGTGATCCGTCGGATGCTTCTGAGCTTGCAGCGAAATTGAGATCCAAGGACAAACTTCTTGTAGCGTACTTTGGGGATGACACTTTTGCGTGGTGCCATCCGTCACAATTGAAACCCTTTGAGGAAAATTTTGTGGAGAATTCTAAGCTGAGTAGCTCTAAGGTTTTTGTTATTGCCGTGCAGCAGGCTGTGGATGAGATTGGTAGGCTTGTAAAGTTGAAGATGAGTTGTGCTTGCATGGGGGAAGAGTTTCTAAGTGGTCGTAAACAGCCTTTAGCATCGAATGCTGGAGTTAAGGAAGGAGTTGTTGTCCCTGAAGGCCGAGTTGGGAAGTTTTTGGGTCATCTATCTCAGCCGGTTGACCTTCTTGCGGAATTGAAACATATTGCACAAGTTGTATCCTTGACTAGTGTTCTTGAGCTGAATGTATTAAAGAGTTTGCTGTCTGCCTTTTATTGTTCGAAAGGGGGATATCAGTTACCTGTTTACATTGAACCCCAGCTAATTCCTGGTCTTGAAGATGAGCGGAAGATGGTGGAAGTCCCAGTGCAGTGGCCATCTGGTGACAGGTTGTCTTCTCCCATAGGCTCCAATACTGGAAACGCTAAACAAAATTTGTTGCAAGGGTCACCACCAAGTATAGAAAATAGACAGCCCCATAAAAGGAAGGAGAAGAGCATTGCTGATCTTCTTGGAGAAGCCAATGGTGTTGAGGTAAAAAATATGGATGGGGTTGTAGCTAAAGAAAGTGCAATCCCAAAAAAACGAGGGCTATCATCCAGACGAGAGAAGCAGAAAAGCAGTGATATTCATGATGAGGATAGATTGACTGTCGAATCAGGAAAGGAAAGAGCCAAACTCTCAAAATCGCCCACAAGCACTAAGAAGGCTCCTTTGGCGAGGAGAAGGGAAGAAGATGAAGGTATTGGCATCGACAGTAGTGATATTTTGGCTAAAGAGAGTGCTGGGGGAAATGAAGAAACTAAGAAGACTCCTTTCTCAAGGAGAAGGAAGAGGGATGAAGATAGCATCAACAGTAAAGATGGTGATACCATAGGAAAAAGTGGTGTAAAGGATGAAACTAAGAAGGCTCCGTTGTCAAGGAGAAGGAAGAAGAATGAAGATATTCACATCAACAGTGAGGATGGTAAGGCCAAAGGAAAAGCTGGTGCAAGAGTCGGGGAGTTGCTCAATGTTGGTTTACAAACAGATATAAAAGACCAAATTGGTAAGAGCCCTTTGTTGAGGGAAAGAAAAAAAAGCAAGTACTTGTCCCCACCCTTCACAATAAGTGACGTAGGGAAAAGAAAACGAAACCTGGAAACAGAATCTCCAAATGCTGTAACAAGTCATGATTTGGGGAAAGAGGGAAAAGAGCGAGACATGGAATCAGTATTATCTCTGGAGGTTTCTAGTAAAGCTCAGGTTGGGGAGAGGATTACCGGGGCTGCCGTCAACATTAAGGGGTCCCCTCAGATTTTGAATAGTTGCAGTGAGGCATTACAAGAGAAACTCTCAATAGAAGGTGGGAATAACAGCACTGATGTTAAAACACCAGCAGAAGGTGAGAAGCGTGTTGATCCAATAAAAGCTAATGCAGCAACTAATGAAGTGCTATCTGGAGTCTGCTCTGCAGCTGTTGATCCATTATATCCAGTCAAAACGAAATCCTCCTTTGAGATTGTTGTTGATTTTGTGTCCATATTTAGAGACTCAGTTTACCACAATGGCTGCTACCACAAGCTTTACAAGAAGCACCAACCTCATGGAAAGAGGAAGAGATCAGATTCTGAACTGGGGTCATTGGTGAAAGACAATCAAACTGCTGCAAATTTGCGTGAAGGTGAATCTGGTAACAGGGTTGTTAAGAAGAAACAAGATATGAAGTCTGATAAGCCTACAAAAAAGCAAGCTAGTGAGACACGAAGTTCAAAGAGAAGGAAGTTAAATTCTGTAATTAAGTCGTTGGGAAAAGACAATGAGAAAGCAGAAAATCAGCCTGTCAGTGAATCTAGGCAGAGGAACATCAACACAAACCAAGATAAGCCTAAGAAAAAGCCAGCTGCTGAGCTACCAATTTCAAAGCTGGTCAGGCGGAGATCAAAGCATGCTGATGCTCGGCATTTGAAGATTAAGGAGACGGAAGAGGAGGCGCCGCCTACATTCCTTTTTGTAACATTTGGACCTGGATCCTCGCTGCCTACCAAGGATGATCTTCTCAGATTATACAGTAAATTTGGGGAACTAAATGAAACTGAAACTGAGATGTTCTACACCAATTTCTGTGCTCGTGTTGCGTTTGTAATGAGCTCTGACGCAGAAGAAGCCTTCAATCATTCTCTAATTGACAGTCCCTTTGGAGCTTCCAATGTCAATTTCCGCCTCCAAAATCCCTCGGGTGCTTCCAAGACTCGTGAGCTCAGTGCAATACCCGAGTCTCCTCCAGCTAAGAAGAAGTCCAGGGGCAAGACTGCAAGTGAGGCATTAGTAGCTTCACAACCACCTGCTGGTGAGCCATCGCAAGTTGATTTCATTAGGCATAGACTTCAGATGATGAGCTCAATGCTGGGAAATTCAGATCGCAAAGTGTCGCCTGTTGCAACATCGAAAATTCAGAATGAGATAAAGGAGCTACTGAAGGTCGTAAGCACAATGGGTGAATCCTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1091

Amino Acids

118.56

Weight (kDa)

8.56

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 183 - 266 1.9e-20 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3112
Acc36I ACCTGC 1 cut(s) 3112
AccB1I GGYRCC 2 cut(s) 693, 2752
AccB7I CCANNNNNTGG 1 cut(s) 3107
AccBSI CCGCTC 1 cut(s) 1126
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 5 cut(s) 971, 1126, 2695, 2756, 2980
AclWI GGATC 6 cut(s) 599, 636, 2174, 2237, 2784, 2797
AcoI YGGCCR 2 cut(s) 587, 1154
AcsI RAATTY 9 cut(s) 322, 727, 739, 1213, 2431, 2548, 2834, 3172, 3210
AcuI CTGAAG 3 cut(s) 936, 3131, 3257
AcyI GRCGYC 1 cut(s) 2753
AfaI GTAC 3 cut(s) 481, 668, 1868
AfiI CCNNNNNNNGG 5 cut(s) 476, 970, 1263, 2162, 3107
AflIII ACRYGT 1 cut(s) 227
AhdI GACNNNNNGTC 1 cut(s) 1171
AhlI ACTAGT 1 cut(s) 1007
AjnI CCWGG 4 cut(s) 1108, 1917, 2785, 3059
AjuI GAANNNNNNNTTGG 6 cut(s) 909, 941, 1478, 1510, 2960, 2992
AloI GAACNNNNNNTCC 2 cut(s) 450, 482
Alw21I GWGCWC 4 cut(s) 2890, 2912, 3021, 3161
Alw26I GTCTC 5 cut(s) 1974, 2304, 2513, 2732, 3042
AlwI GGATC 6 cut(s) 599, 636, 2174, 2237, 2784, 2797
AlwNI CAGNNNCTG 2 cut(s) 2390, 2666
Ama87I CYCGRG 3 cut(s) 115, 2995, 3032
Aor13HI TCCGGA 1 cut(s) 137
AoxI GGCC 6 cut(s) 84, 587, 592, 919, 1154, 1752
ApeKI GCWGC 9 cut(s) 629, 781, 2048, 2198, 2234, 2331, 2426, 2663, 2797
ApoI RAATTY 9 cut(s) 322, 727, 739, 1213, 2431, 2548, 2834, 3172, 3210
ArsI GACNNNNNNTTYG 2 cut(s) 2939, 2971
Asp700I GAANNNNTTC 3 cut(s) 536, 849, 1725
AspLEI GCGC 1 cut(s) 2755
AspS9I GGNCC 5 cut(s) 68, 135, 592, 2067, 2783
AsuC2I CCSGG 5 cut(s) 55, 88, 128, 591, 2044
AsuII TTCGAA 2 cut(s) 314, 1061
AvaI CYCGRG 3 cut(s) 115, 2995, 3032
AvaII GGWCC 4 cut(s) 68, 135, 2067, 2783
BalI TGGCCA 1 cut(s) 1156
BamHI GGATCC 1 cut(s) 2789
BanI GGYRCC 2 cut(s) 693, 2752
BanII GRGCYC 4 cut(s) 1838, 2912, 3021, 3161
BauI CACGAG 2 cut(s) 2889, 3012
BbsI GAAGAC 4 cut(s) 323, 504, 1167, 1595
Bbv12I GWGCWC 4 cut(s) 2890, 2912, 3021, 3161
BbvI GCAGC 9 cut(s) 641, 793, 2035, 2210, 2246, 2318, 2413, 2650, 2784
BceAI ACGGC 3 cut(s) 11, 761, 2036
BciT130I CCWGG 4 cut(s) 1110, 1919, 2787, 3061
BciVI GTATCC 2 cut(s) 217, 1009
BcnI CCSGG 5 cut(s) 55, 88, 128, 591, 2044
BcoDI GTCTC 5 cut(s) 1974, 2304, 2513, 2732, 3042
BcuI ACTAGT 1 cut(s) 1007
BfaI CTAG 5 cut(s) 449, 1008, 2013, 2514, 2613
BfmI CTRYAG 2 cut(s) 21, 2232
BfoI RGCGCY 1 cut(s) 2756
BfuAI ACCTGC 1 cut(s) 3112
BfuI GTATCC 2 cut(s) 217, 1009
BglII AGATCT 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 2667
BmcAI AGTACT 1 cut(s) 1868
Bme1390I CCNGG 9 cut(s) 55, 88, 128, 591, 1110, 1919, 2044, 2787, 3061
Bme18I GGWCC 4 cut(s) 68, 135, 2067, 2783
BmeRI GACNNNNNGTC 1 cut(s) 1171
BmeT110I CYCGRG 3 cut(s) 115, 2995, 3032
BmgT120I GGNCC 5 cut(s) 68, 135, 592, 2067, 2783
BmrFI CCNGG 9 cut(s) 55, 88, 128, 591, 1110, 1919, 2044, 2787, 3061
BmrI ACTGGG 3 cut(s) 511, 1139, 2405
BmsI GCATC 6 cut(s) 319, 604, 887, 1539, 1638, 2704
BmuI ACTGGG 3 cut(s) 511, 1139, 2405
BpiI GAAGAC 4 cut(s) 323, 504, 1167, 1595
BplI GAGNNNNNCTC 4 cut(s) 1443, 1475, 2099, 2131
BpmI CTGGAG 5 cut(s) 290, 909, 2024, 2241, 3027
Bpu10I CCTNAGC 1 cut(s) 2022
Bpu1102I GCTNAGC 1 cut(s) 2667
Bpu14I TTCGAA 2 cut(s) 314, 1061
BpuEI CTTGAG 2 cut(s) 1037, 1585
BpuMI CCSGG 5 cut(s) 55, 88, 128, 591, 2044
BsaAI YACGTR 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 2753
BsaJI CCNNGG 7 cut(s) 87, 581, 645, 2043, 2805, 2994, 3060
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 8 cut(s) 241, 271, 540, 570, 894, 924, 1915, 1945
Bsc4I CCNNNNNNNGG 5 cut(s) 476, 970, 1263, 2162, 3107
Bse118I RCCGGY 3 cut(s) 70, 476, 955
Bse1I ACTGG 5 cut(s) 506, 1145, 1201, 2255, 2400
Bse3DI GCAATG 2 cut(s) 471, 1278
BseAI TCCGGA 1 cut(s) 137
BseBI CCWGG 4 cut(s) 1110, 1919, 2787, 3061
BseDI CCNNGG 7 cut(s) 87, 581, 645, 2043, 2805, 2994, 3060
BseLI CCNNNNNNNGG 5 cut(s) 476, 970, 1263, 2162, 3107
BseMI GCAATG 2 cut(s) 471, 1278
BseNI ACTGG 5 cut(s) 506, 1145, 1201, 2255, 2400
BseRI GAGGAG 9 cut(s) 107, 112, 115, 302, 307, 425, 1516, 2762, 3030
BseXI GCAGC 9 cut(s) 641, 793, 2035, 2210, 2246, 2318, 2413, 2650, 2784
BseYI CCCAGC 3 cut(s) 1098, 1565, 3166
BsgI GTGCAG 2 cut(s) 800, 1169
BshFI GGCC 6 cut(s) 86, 589, 594, 921, 1156, 1754
BshNI GGYRCC 2 cut(s) 693, 2752
BsiHKAI GWGCWC 4 cut(s) 2890, 2912, 3021, 3161
BsiHKCI CYCGRG 3 cut(s) 115, 2995, 3032
BsiSI CCGG 9 cut(s) 54, 71, 87, 127, 138, 477, 590, 956, 2043
BslFI GGGAC 7 cut(s) 19, 230, 896, 1127, 1858, 2053, 2937
BslI CCNNNNNNNGG 5 cut(s) 476, 970, 1263, 2162, 3107
BsmAI GTCTC 5 cut(s) 1974, 2304, 2513, 2732, 3042
BsmBI CGTCTC 1 cut(s) 2732
BsmFI GGGAC 7 cut(s) 19, 230, 896, 1127, 1858, 2053, 2937
BsmI GAATGC 1 cut(s) 889
BsnI GGCC 6 cut(s) 86, 589, 594, 921, 1156, 1754
BsoBI CYCGRG 3 cut(s) 115, 2995, 3032
Bsp119I TTCGAA 2 cut(s) 314, 1061
Bsp1286I GDGCHC 5 cut(s) 1838, 2890, 2912, 3021, 3161
Bsp13I TCCGGA 1 cut(s) 137
Bsp1720I GCTNAGC 1 cut(s) 2667
BspACI CCGC 5 cut(s) 971, 1126, 2695, 2756, 2980
BspANI GGCC 6 cut(s) 86, 589, 594, 921, 1156, 1754
BspEI TCCGGA 1 cut(s) 137
BspHI TCATGA 2 cut(s) 1411, 1951
BspMAI CTGCAG 1 cut(s) 2236
BspMI ACCTGC 1 cut(s) 3112
BspPI GGATC 6 cut(s) 599, 636, 2174, 2237, 2784, 2797
BspQI GCTCTTC 2 cut(s) 438, 1268
BspT104I TTCGAA 2 cut(s) 314, 1061
BspT107I GGYRCC 2 cut(s) 693, 2752
BsrBI CCGCTC 1 cut(s) 1126
BsrDI GCAATG 2 cut(s) 471, 1278
BsrFI RCCGGY 3 cut(s) 70, 476, 955
BsrI ACTGG 5 cut(s) 506, 1145, 1201, 2255, 2400
BssAI RCCGGY 3 cut(s) 70, 476, 955
BssECI CCNNGG 7 cut(s) 87, 581, 645, 2043, 2805, 2994, 3060
BssNI GRCGYC 1 cut(s) 2753
BssSI CACGAG 2 cut(s) 2889, 3012
BssT1I CCWWGG 2 cut(s) 645, 2805
Bst2BI CACGAG 2 cut(s) 2889, 3012
Bst2UI CCWGG 4 cut(s) 1110, 1919, 2787, 3061
Bst4CI ACNGT 8 cut(s) 181, 389, 1432, 1538, 1637, 1739, 2831, 2951
Bst6I CTCTTC 7 cut(s) 438, 507, 840, 1268, 1607, 2372, 2739
BstACI GRCGYC 1 cut(s) 2753
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBAI YACGTR 1 cut(s) 228
BstBI TTCGAA 2 cut(s) 314, 1061
BstC8I GCNNGC 6 cut(s) 627, 786, 835, 2511, 2661, 2709
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 1227
BstH2I RGCGCY 1 cut(s) 2756
BstHHI GCGC 1 cut(s) 2755
BstMAI GTCTC 5 cut(s) 1974, 2304, 2513, 2732, 3042
BstMWI GCNNNNNNNGC 8 cut(s) 884, 1496, 2198, 2692, 2894, 2924, 3080, 3089
BstNI CCWGG 4 cut(s) 1110, 1919, 2787, 3061
BstNSI RCATGY 1 cut(s) 2711
BstPI GGTNACC 1 cut(s) 1227
BstSCI CCNGG 9 cut(s) 53, 86, 126, 589, 1108, 1917, 2042, 2785, 3059
BstSFI CTRYAG 2 cut(s) 21, 2232
BstV1I GCAGC 9 cut(s) 641, 793, 2035, 2210, 2246, 2318, 2413, 2650, 2784
BstV2I GAAGAC 4 cut(s) 323, 504, 1167, 1595
BstX2I RGATCY 3 cut(s) 17, 641, 2789
BstXI CCANNNNNNTGG 2 cut(s) 1152, 2366
BstYI RGATCY 3 cut(s) 17, 641, 2789
BsuI GTATCC 2 cut(s) 217, 1009
BsuRI GGCC 6 cut(s) 86, 589, 594, 921, 1156, 1754
BtgI CCRYGG 1 cut(s) 581
BtgZI GCGATG 1 cut(s) 3102
BtsI GCAGTG 3 cut(s) 1157, 1408, 2098
BtsIMutI CAGTG 8 cut(s) 1152, 1157, 1408, 1744, 2098, 2139, 2611, 3028
BveI ACCTGC 1 cut(s) 3112
Cac8I GCNNGC 6 cut(s) 627, 786, 835, 2511, 2661, 2709
CaiI CAGNNNCTG 2 cut(s) 2390, 2666
CciI TCATGA 2 cut(s) 1411, 1951
CfoI GCGC 1 cut(s) 2755
Cfr10I RCCGGY 3 cut(s) 70, 476, 955
Cfr13I GGNCC 5 cut(s) 68, 135, 592, 2067, 2783
CseI GACGC 1 cut(s) 2924
Csp6I GTAC 3 cut(s) 480, 667, 1867
CspCI CAANNNNNGTGG 2 cut(s) 373, 408
CviAII CATG 7 cut(s) 220, 838, 1412, 1952, 1984, 2366, 2708
CviQI GTAC 3 cut(s) 480, 667, 1867
DinI GGCGCC 1 cut(s) 2754
DriI GACNNNNNGTC 1 cut(s) 1171
EaeI YGGCCR 2 cut(s) 587, 1154
Eam1104I CTCTTC 7 cut(s) 438, 507, 840, 1268, 1607, 2372, 2739
Eam1105I GACNNNNNGTC 1 cut(s) 1171
EarI CTCTTC 7 cut(s) 438, 507, 840, 1268, 1607, 2372, 2739
EciI GGCGGA 2 cut(s) 2710, 2969
Ecl136II GAGCTC 3 cut(s) 2910, 3019, 3159
Eco130I CCWWGG 2 cut(s) 645, 2805
Eco24I GRGCYC 4 cut(s) 1838, 2912, 3021, 3161
Eco32I GATATC 1 cut(s) 1073
Eco47I GGWCC 4 cut(s) 68, 135, 2067, 2783
Eco53kI GAGCTC 3 cut(s) 2910, 3019, 3159
Eco57I CTGAAG 3 cut(s) 936, 3131, 3257
Eco88I CYCGRG 3 cut(s) 115, 2995, 3032
Eco91I GGTNACC 1 cut(s) 1227
EcoICRI GAGCTC 3 cut(s) 2910, 3019, 3159
EcoO109I RGGNCCY 1 cut(s) 2067
EcoO65I GGTNACC 1 cut(s) 1227
EcoRI GAATTC 2 cut(s) 322, 739
EcoRII CCWGG 4 cut(s) 1108, 1917, 2785, 3059
EcoRV GATATC 1 cut(s) 1073
EcoT14I CCWWGG 2 cut(s) 645, 2805
EcoT38I GRGCYC 4 cut(s) 1838, 2912, 3021, 3161
EgeI GGCGCC 1 cut(s) 2754
EheI GGCGCC 1 cut(s) 2754
ErhI CCWWGG 2 cut(s) 645, 2805
Esp3I CGTCTC 1 cut(s) 2732
FaeI CATG 7 cut(s) 223, 841, 1415, 1955, 1987, 2369, 2711
FaqI GGGAC 7 cut(s) 19, 230, 896, 1127, 1858, 2053, 2937
FatI CATG 7 cut(s) 219, 837, 1411, 1951, 1983, 2365, 2707
FriOI GRGCYC 4 cut(s) 1838, 2912, 3021, 3161
FspBI CTAG 5 cut(s) 449, 1008, 2013, 2514, 2613
GlaI GCGC 1 cut(s) 2754
GsaI CCCAGC 3 cut(s) 1102, 1569, 3170
GsuI CTGGAG 5 cut(s) 290, 909, 2024, 2241, 3027
HaeII RGCGCY 1 cut(s) 2756
HaeIII GGCC 6 cut(s) 86, 589, 594, 921, 1156, 1754
HapII CCGG 9 cut(s) 54, 71, 87, 127, 138, 477, 590, 956, 2043
HgaI GACGC 1 cut(s) 2924
HhaI GCGC 1 cut(s) 2755
Hin1I GRCGYC 1 cut(s) 2753
Hin1II CATG 7 cut(s) 223, 841, 1415, 1955, 1987, 2369, 2711
Hin6I GCGC 1 cut(s) 2753
HinP1I GCGC 1 cut(s) 2753
HincII GTYRAC 2 cut(s) 961, 2056
HindII GTYRAC 2 cut(s) 961, 2056
HindIII AAGCTT 1 cut(s) 2342
HpaII CCGG 9 cut(s) 54, 71, 87, 127, 138, 477, 590, 956, 2043
Hpy166II GTNNAC 6 cut(s) 866, 961, 1087, 1802, 2056, 2320
Hpy8I GTNNAC 6 cut(s) 866, 961, 1087, 1802, 2056, 2320
Hpy99I CGWCG 2 cut(s) 404, 613
HpyCH4III ACNGT 8 cut(s) 181, 389, 1432, 1538, 1637, 1739, 2831, 2951
HpyCH4IV ACGT 3 cut(s) 8, 227, 1896
HpyF10VI GCNNNNNNNGC 8 cut(s) 884, 1496, 2198, 2692, 2894, 2924, 3080, 3089
HpySE526I ACGT 3 cut(s) 8, 227, 1896
Hsp92I GRCGYC 1 cut(s) 2753
Hsp92II CATG 7 cut(s) 223, 841, 1415, 1955, 1987, 2369, 2711
HspAI GCGC 1 cut(s) 2753
KasI GGCGCC 1 cut(s) 2752
KflI GGGWCCC 1 cut(s) 2067
Kpn2I TCCGGA 1 cut(s) 137
LguI GCTCTTC 2 cut(s) 438, 1268
LmnI GCTCC 8 cut(s) 56, 123, 145, 1193, 1495, 1696, 2960, 3229
Lsp1109I GCAGC 9 cut(s) 641, 793, 2035, 2210, 2246, 2318, 2413, 2650, 2784
LweI GCATC 6 cut(s) 319, 604, 887, 1539, 1638, 2704
MaeI CTAG 5 cut(s) 449, 1008, 2013, 2514, 2613
MaeII ACGT 3 cut(s) 8, 227, 1896
MaeIII GTNAC 8 cut(s) 702, 1077, 1163, 1227, 1892, 1942, 2453, 2773
MbiI CCGCTC 1 cut(s) 1126
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 3 cut(s) 17, 641, 2789
MhlI GDGCHC 5 cut(s) 1838, 2890, 2912, 3021, 3161
MlsI TGGCCA 1 cut(s) 1156
MluNI TGGCCA 1 cut(s) 1156
Mly113I GGCGCC 1 cut(s) 2753
MlyI GAGTC 8 cut(s) 107, 281, 1585, 1785, 2232, 2306, 3004, 3044
MmeI TCCRAC 2 cut(s) 241, 591
Mox20I TGGCCA 1 cut(s) 1156
MroI TCCGGA 1 cut(s) 137
MroXI GAANNNNTTC 3 cut(s) 536, 849, 1725
MscI TGGCCA 1 cut(s) 1156
MslI CAYNNNNRTG 1 cut(s) 2712
Msp20I TGGCCA 1 cut(s) 1156
MspA1I CMGCKG 2 cut(s) 2237, 2663
MspI CCGG 9 cut(s) 54, 71, 87, 127, 138, 477, 590, 956, 2043
MspR9I CCNGG 9 cut(s) 55, 88, 128, 591, 1110, 1919, 2044, 2787, 3061
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 889
MvaI CCWGG 4 cut(s) 1110, 1919, 2787, 3061
MwoI GCNNNNNNNGC 8 cut(s) 884, 1496, 2198, 2692, 2894, 2924, 3080, 3089
NarI GGCGCC 1 cut(s) 2753
NciI CCSGG 5 cut(s) 55, 88, 128, 591, 2044
NlaIII CATG 7 cut(s) 223, 841, 1415, 1955, 1987, 2369, 2711
NmeAIII GCCGAG 2 cut(s) 947, 2698
NmuCI GTSAC 4 cut(s) 702, 1163, 1227, 1892
NspI RCATGY 1 cut(s) 2711
NspV TTCGAA 2 cut(s) 314, 1061
PaeI GCATGC 1 cut(s) 2711
PagI TCATGA 2 cut(s) 1411, 1951
PaqCI CACCTGC 1 cut(s) 3112
PciSI GCTCTTC 2 cut(s) 438, 1268
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PctI GAATGC 1 cut(s) 889
PdmI GAANNNNTTC 3 cut(s) 536, 849, 1725
PfeI GAWTC 7 cut(s) 1436, 1928, 1988, 2387, 2447, 2609, 3260
PflMI CCANNNNNTGG 1 cut(s) 3107
PleI GAGTC 8 cut(s) 107, 280, 1585, 1784, 2231, 2306, 3004, 3043
PluTI GGCGCC 1 cut(s) 2756
PpsI GAGTC 8 cut(s) 107, 280, 1585, 1784, 2231, 2306, 3004, 3043
Ppu21I YACGTR 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 2067
Psp124BI GAGCTC 3 cut(s) 2912, 3021, 3161
Psp5II RGGWCCY 1 cut(s) 2067
Psp6I CCWGG 4 cut(s) 1108, 1917, 2785, 3059
PspEI GGTNACC 1 cut(s) 1227
PspFI CCCAGC 3 cut(s) 1098, 1565, 3166
PspGI CCWGG 4 cut(s) 1108, 1917, 2785, 3059
PspPI GGNCC 5 cut(s) 68, 135, 592, 2067, 2783
PspPPI RGGWCCY 1 cut(s) 2067
PstI CTGCAG 1 cut(s) 2236
PstNI CAGNNNCTG 2 cut(s) 2390, 2666
PsuI RGATCY 3 cut(s) 17, 641, 2789
PvuII CAGCTG 2 cut(s) 2237, 2663
RsaI GTAC 3 cut(s) 481, 668, 1868
RsaNI GTAC 3 cut(s) 480, 667, 1867
RseI CAYNNNNRTG 1 cut(s) 2712
SacI GAGCTC 3 cut(s) 2912, 3021, 3161
SapI GCTCTTC 2 cut(s) 438, 1268
Sau96I GGNCC 5 cut(s) 68, 135, 592, 2067, 2783
ScaI AGTACT 1 cut(s) 1868
SchI GAGTC 8 cut(s) 107, 281, 1585, 1785, 2232, 2306, 3004, 3044
ScrFI CCNGG 9 cut(s) 55, 88, 128, 591, 1110, 1919, 2044, 2787, 3061
SduI GDGCHC 5 cut(s) 1838, 2890, 2912, 3021, 3161
SfaNI GCATC 6 cut(s) 319, 604, 887, 1539, 1638, 2704
SfcI CTRYAG 2 cut(s) 21, 2232
SfoI GGCGCC 1 cut(s) 2754
SfuI TTCGAA 2 cut(s) 314, 1061
SinI GGWCC 4 cut(s) 68, 135, 2067, 2783
SmiMI CAYNNNNRTG 1 cut(s) 2712
SmlI CTYRAG 2 cut(s) 1016, 1600
SmoI CTYRAG 2 cut(s) 1016, 1600
SpeI ACTAGT 1 cut(s) 1007
SphI GCATGC 1 cut(s) 2711
SsiI CCGC 5 cut(s) 971, 1126, 2695, 2756, 2980
SspDI GGCGCC 1 cut(s) 2752
SspMI CTAG 5 cut(s) 449, 1008, 2013, 2514, 2613
SstI GAGCTC 3 cut(s) 2912, 3021, 3161
StyD4I CCNGG 9 cut(s) 53, 86, 126, 589, 1108, 1917, 2042, 2785, 3059
StyI CCWWGG 2 cut(s) 645, 2805
TaaI ACNGT 8 cut(s) 181, 389, 1432, 1538, 1637, 1739, 2831, 2951
TaiI ACGT 3 cut(s) 11, 230, 1899
TaqI TCGA 7 cut(s) 236, 314, 881, 1061, 1434, 1533, 3206
TatI WGTACW 1 cut(s) 1866
TauI GCSGC 1 cut(s) 2758
TfiI GAWTC 7 cut(s) 1436, 1928, 1988, 2387, 2447, 2609, 3260
TscAI CASTG 8 cut(s) 1152, 1157, 1408, 1744, 2098, 2146, 2611, 3028
TseFI GTSAC 4 cut(s) 702, 1163, 1227, 1892
TseI GCWGC 9 cut(s) 629, 781, 2048, 2198, 2234, 2331, 2426, 2663, 2797
Tsp45I GTSAC 4 cut(s) 702, 1163, 1227, 1892
TspGWI ACGGA 4 cut(s) 597, 690, 1683, 2756
TspRI CASTG 8 cut(s) 1152, 1157, 1408, 1744, 2098, 2146, 2611, 3028
Van91I CCANNNNNTGG 1 cut(s) 3107
VpaK11BI GGWCC 4 cut(s) 68, 135, 2067, 2783
XapI RAATTY 9 cut(s) 322, 727, 739, 1213, 2431, 2548, 2834, 3172, 3210
XceI RCATGY 1 cut(s) 2711
XcmI CCANNNNNNNNNTGG 1 cut(s) 1106
XmnI GAANNNNTTC 3 cut(s) 536, 849, 1725
XspI CTAG 5 cut(s) 449, 1008, 2013, 2514, 2613
ZrmI AGTACT 1 cut(s) 1868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.