pycom111g02480

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0002210
Physical Location & Seq
Forward (+)
1474140 .. 1476488
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02480.1

Sequence Viewer

Length: 2349 bp
ATGGATGATGAGGGACATGAGTTTCTTGTTGGTGATTTTGTGTGGGGAAAGATTAAGAGCCATCCGTGGTGGCCTGCACAAATTTGTGATCCTTCGGACGCTTCGGAGTATGCCCTGAAATTGAAGGCCAAAGACAGACTCCTGGTGGCGTATTTTGGGGACGGAACATTTGCTTGGTGCAATTCATTGCAGCTGAAACCGTTTGAGGAGAACTTTAGGGAGATGTCGGGGCAGAGTAGCTCGAAGGCGTTTGTTAATGCTGTGCAGCAGGCTGTGGATGAGGTTGGAAGGATTGTGATGTTGAAGATGAGTTGTGGGTGTGTAAAGGAAGAGTCCCTGAGTGAGGTTGGTCGGCCGTTGGCAGTAAATGCCGGAATTAAGGAAGGAGTTCTTGTGCCAGAAGGCAGGGTTGGGAAGCTTTTGGATCGTCTGTCTGACCCGGCAGAGCTTCTTGCTGAGTTGAAACATGTTGCAGAAGTTATGTCCACGTCTAGTGAGCTTCAGCTGAATGCTTTGAAGAGTTGGTTGTCGGCATATTATTGTTCAAAAGGGGGCTATCATTTGCCTGTTTTCGTCGAAGCGCAGCCAGTTGCTGGTCTTGAGGATGACTGGAGGGCGGTGGATGTCCCGCACCAAGGGCCATTTGAAGACTGGCTTTCTTCTCCCAGAAAATCAGGTCAAATTGATCAACCTTTACACGAAAATTCGGCTCAAGGTTTAGAGAATAGGCAATACCAAAGAAGGAAGCAGAAGAGCATTGCTGATATAATGGGAGAGGATGATGATATTCAGGCGGAAACAAAGGAAGGAGCAACATCAGAGAAAGCAGGGGTATCATCTGGGCCGAAGAAGCGGAAAGTTGGCGAGAATCATGGTGAGAGTAATTTGACTTCTGAATCCGGAAAGAGAAGGGCCAAGCTTTCAAAGACACCAACGAGCACGCAGATGAAAAAACTATCATGTGCTGAAAATGGTTCTAGTGACCGTACGGAAGAAACTAAGAAGGGTGCCTTAACAAGGAGCAGGAAGAAGGCTGAAGGTACTGGAATTGAAAGCAATGGTGGCGAGACCAAAGAAGAAGCCGGTGATAGTCCTATTCTGCGCAGTGGCGGTTCACAAACTGACATGAAAGACCAAATTGATCACCCCTTTTCCACAAGAGAGAGGAAAAGGAGCAAGTACTTGTCCCCTCCCTTCATAAATCTAAGCACTGGAAAAAGGAGTCTAGACATAGAAGTAGAATCTCAGAAAGTTTATAATGAGAACCTTGTTGGGTCCCCGAAGATGCTGAATCCTTGCACAGAGACGTTACAGAAGAAAGACTCCACAGAACTTGGGCTTGGGAATGAGATATCTGGTGGCTCAAGTTCGAAAAAACCATCAGCAGATGACAAGAAGAGCATTGATGAGATGAAAGCTAATGTATCTAATCGTAACGTGCTATCTGGAGTCCGCTCTGCAGCTGTTAATCCATCATCTCCAATCAAAAAGAAATCCTTTGAGATTGTTAAGGATTTTCTGTCCATATTCAGAGACTCGATTTATCGCAATGGATCCTACTACGACATATACAAGAAGAAGCAACCTGATAAGAAGAGAAAGAAGTTAGAATCTGAACCTGGCTCAATGGGGAAAGACCGAAATCAGACTGCAGAAAACCTGCCTGAAACTGAATCTAGGAAGAAGATAATCAAGAAGAGCAGTGAGACAAGGTCTGCTAAGCCAACACAAAAGCAAGCTACTGAGACACCAGGTTCAGAGCCTGGTAGTAAGCGAAAATCAAAGCATGCTTCTGGAACTCCGGATTTGAAGAAAAGGCGTAGAAAGACCGACGAAATAGCTTCACCTGCATCCCTTTTTGTGACATTTGGCCCTGGGTCCAATCTCCCCACAAAAGCTGATCTTATCAAGATTTATAGTAAGTTCGGAGAGCTGAACGAAATGGAAACTGAGATGTTCTACACCAATTTCTGCGCTCGTGTTTCCTTTGCAAGGCTAGCTGATGCAGAAGAAGCCTTCAACCATTCGCAAAATGACAGTCCGTTTGGAGCTTCCAACGTCAATTTCCGGCTTCATAATCTGGCAGCTGCTTCGAAGGTTCGTGAACTGAGTGAAATATCCAATTCTGCTCCTGCTAAGAAGTCCAGGGGCAAGACCAAAACCCAGGCATTAGCTTCACAGGTACCCGCTGCTGCTGGTGAGGCGTCGCAGATCGACTTAATCAAGCAGAAACTGGAGAAGATGACCTCAATGCTGGATGATTCAAATGGCCAAGTTTCGGATGTCACGAAATCGAAACTGGAGAGCGAGATAAAAGAGCTGTTGGGGACGGTAAGCACGATGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

783

Amino Acids

85.96

Weight (kDa)

8.92

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 11 - 94 2.9e-21 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1257
AarI CACCTGC 1 cut(s) 1855
Acc16I TGCGCA 1 cut(s) 1103
Acc36I ACCTGC 2 cut(s) 1668, 1855
Acc65I GGTACC 1 cut(s) 2182
AccB1I GGYRCC 2 cut(s) 1007, 2182
AccB7I CCANNNNNTGG 1 cut(s) 593
AccBSI CCGCTC 1 cut(s) 1455
AccIII TCCGGA 2 cut(s) 899, 1801
AciI CCGC 7 cut(s) 617, 629, 794, 853, 1110, 1453, 2187
AclWI GGATC 4 cut(s) 83, 432, 1548, 1561
AcoI YGGCCR 2 cut(s) 353, 2269
AcsI RAATTY 2 cut(s) 81, 703
AcuI CTGAAG 2 cut(s) 485, 1056
AcyI GRCGYC 1 cut(s) 2204
AfaI GTAC 4 cut(s) 988, 1042, 1181, 2184
AfiI CCNNNNNNNGG 6 cut(s) 343, 593, 635, 1017, 1992, 2278
AflIII ACRYGT 1 cut(s) 466
AjiI CACGTC 1 cut(s) 489
AjnI CCWGG 7 cut(s) 141, 1618, 1750, 1762, 1873, 2144, 2163
AjuI GAANNNNNNNTTGG 8 cut(s) 157, 189, 393, 425, 1323, 1355, 2048, 2080
Alw21I GWGCWC 1 cut(s) 941
Alw26I GTCTC 5 cut(s) 1061, 1298, 1527, 1700, 1739
AlwI GGATC 4 cut(s) 83, 432, 1548, 1561
AlwNI CAGNNNCTG 3 cut(s) 593, 1763, 2233
Aor13HI TCCGGA 2 cut(s) 899, 1801
AoxI GGCC 8 cut(s) 71, 126, 353, 638, 842, 912, 1870, 2269
ApeKI GCWGC 8 cut(s) 190, 265, 583, 1460, 2084, 2087, 2189, 2192
ApoI RAATTY 2 cut(s) 81, 703
ArsI GACNNNNNNTTYG 6 cut(s) 152, 184, 2023, 2027, 2055, 2059
Asp700I GAANNNNTTC 1 cut(s) 387
Asp718I GGTACC 1 cut(s) 2182
AspLEI GCGC 3 cut(s) 583, 1104, 1976
AspS9I GGNCC 6 cut(s) 638, 842, 912, 1275, 1871, 1878
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 6 cut(s) 44, 887, 1097, 1136, 1836, 2210
AsuII TTCGAA 2 cut(s) 1370, 2093
AsuNHI GCTAGC 1 cut(s) 1996
AvaII GGWCC 2 cut(s) 1275, 1878
BalI TGGCCA 1 cut(s) 2271
BamHI GGATCC 1 cut(s) 1553
BanI GGYRCC 2 cut(s) 1007, 2182
BarI GAAGNNNNNNTAC 2 cut(s) 874, 906
BauI CACGAG 1 cut(s) 1977
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 941
BbvI GCAGC 8 cut(s) 202, 277, 595, 1472, 2074, 2096, 2176, 2179
BccI CCATC 4 cut(s) 69, 1387, 1480, 2335
BceAI ACGGC 1 cut(s) 340
BcgI CGANNNNNNTGC 2 cut(s) 2073, 2107
BciT130I CCWGG 7 cut(s) 143, 1620, 1752, 1764, 1875, 2146, 2165
BclI TGATCA 2 cut(s) 685, 1141
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 5 cut(s) 1061, 1298, 1527, 1700, 1739
BfaI CTAG 5 cut(s) 492, 978, 1226, 1677, 1997
BfmI CTRYAG 2 cut(s) 1458, 1650
BfuAI ACCTGC 2 cut(s) 1668, 1855
BisI GCNGC 8 cut(s) 191, 266, 584, 1461, 2085, 2088, 2190, 2193
BlpI GCTNAGC 1 cut(s) 1719
BlsI GCNGC 8 cut(s) 192, 267, 585, 1462, 2086, 2089, 2191, 2194
BmcAI AGTACT 1 cut(s) 1181
Bme1390I CCNGG 8 cut(s) 143, 440, 1620, 1752, 1764, 1875, 2146, 2165
Bme18I GGWCC 2 cut(s) 1275, 1878
BmgBI CACGTC 1 cut(s) 489
BmgT120I GGNCC 6 cut(s) 638, 842, 912, 1275, 1871, 1878
BmiI GGNNCC 6 cut(s) 1009, 1276, 1277, 1555, 1879, 2184
BmrFI CCNGG 8 cut(s) 143, 440, 1620, 1752, 1764, 1875, 2146, 2165
BmsI GCATC 3 cut(s) 1275, 1859, 1993
BmtI GCTAGC 1 cut(s) 2000
BpiI GAAGAC 1 cut(s) 654
BpmI CTGGAG 4 cut(s) 631, 1467, 2255, 2321
Bpu1102I GCTNAGC 1 cut(s) 1719
Bpu14I TTCGAA 2 cut(s) 1370, 2093
BpuEI CTTGAG 3 cut(s) 620, 696, 1348
BpuMI CCSGG 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 2204
BsaI GGTCTC 1 cut(s) 1061
BsaJI CCNNGG 6 cut(s) 65, 634, 1873, 1874, 2145, 2163
BsaWI WCCGGW 2 cut(s) 899, 1801
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 6 cut(s) 343, 593, 635, 1017, 1992, 2278
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 7 cut(s) 587, 614, 656, 1048, 1216, 2238, 2304
Bse3DI GCAATG 4 cut(s) 185, 756, 1063, 1555
BseAI TCCGGA 2 cut(s) 899, 1801
BseBI CCWGG 7 cut(s) 143, 1620, 1752, 1764, 1875, 2146, 2165
BseDI CCNNGG 6 cut(s) 65, 634, 1873, 1874, 2145, 2163
BseGI GGATG 9 cut(s) 10, 61, 283, 610, 628, 784, 1850, 2263, 2287
BseLI CCNNNNNNNGG 6 cut(s) 343, 593, 635, 1017, 1992, 2278
BseMI GCAATG 4 cut(s) 185, 756, 1063, 1555
BseMII CTCAG 6 cut(s) 329, 447, 1259, 1734, 1941, 2099
BseNI ACTGG 7 cut(s) 587, 614, 656, 1048, 1216, 2238, 2304
BseRI GAGGAG 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 8 cut(s) 202, 277, 595, 1472, 2074, 2096, 2176, 2179
BsgI GTGCAG 2 cut(s) 60, 284
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 8 cut(s) 73, 128, 355, 640, 844, 914, 1872, 2271
BshNI GGYRCC 2 cut(s) 1007, 2182
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 941
BsiSI CCGG 6 cut(s) 372, 440, 900, 1083, 1802, 2068
BsiWI CGTACG 1 cut(s) 986
BslFI GGGAC 7 cut(s) 27, 173, 319, 611, 1171, 1261, 2341
BslI CCNNNNNNNGG 6 cut(s) 343, 593, 635, 1017, 1992, 2278
BsmAI GTCTC 5 cut(s) 1061, 1298, 1527, 1700, 1739
BsmBI CGTCTC 1 cut(s) 1298
BsmFI GGGAC 7 cut(s) 27, 173, 319, 611, 1171, 1261, 2341
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 8 cut(s) 73, 128, 355, 640, 844, 914, 1872, 2271
Bso31I GGTCTC 1 cut(s) 1061
Bsp119I TTCGAA 2 cut(s) 1370, 2093
Bsp1286I GDGCHC 1 cut(s) 941
Bsp13I TCCGGA 2 cut(s) 899, 1801
Bsp143I GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
Bsp1720I GCTNAGC 1 cut(s) 1719
BspACI CCGC 7 cut(s) 617, 629, 794, 853, 1110, 1453, 2187
BspANI GGCC 8 cut(s) 73, 128, 355, 640, 844, 914, 1872, 2271
BspCNI CTCAG 6 cut(s) 330, 448, 1258, 1735, 1942, 2100
BspEI TCCGGA 2 cut(s) 899, 1801
BspLI GGNNCC 6 cut(s) 1009, 1276, 1277, 1555, 1879, 2184
BspMAI CTGCAG 2 cut(s) 1462, 1654
BspMI ACCTGC 2 cut(s) 1668, 1855
BspOI GCTAGC 1 cut(s) 2000
BspPI GGATC 4 cut(s) 83, 432, 1548, 1561
BspQI GCTCTTC 3 cut(s) 746, 1391, 1691
BspT104I TTCGAA 2 cut(s) 1370, 2093
BspT107I GGYRCC 2 cut(s) 1007, 2182
BspTNI GGTCTC 1 cut(s) 1061
BsrBI CCGCTC 1 cut(s) 1455
BsrDI GCAATG 4 cut(s) 185, 756, 1063, 1555
BsrFI RCCGGY 1 cut(s) 1082
BsrI ACTGG 7 cut(s) 587, 614, 656, 1048, 1216, 2238, 2304
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 6 cut(s) 65, 634, 1873, 1874, 2145, 2163
BssMI GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
BssNI GRCGYC 1 cut(s) 2204
BssSI CACGAG 1 cut(s) 1977
BssT1I CCWWGG 1 cut(s) 634
Bst2BI CACGAG 1 cut(s) 1977
Bst2UI CCWGG 7 cut(s) 143, 1620, 1752, 1764, 1875, 2146, 2165
Bst4CI ACNGT 4 cut(s) 201, 986, 2039, 2332
Bst6I CTCTTC 6 cut(s) 324, 512, 746, 1391, 1589, 1691
BstACI GRCGYC 1 cut(s) 2204
BstAPI GCANNNNNTGC 1 cut(s) 368
BstBI TTCGAA 2 cut(s) 1370, 2093
BstC8I GCNNGC 6 cut(s) 75, 270, 941, 1737, 1788, 1998
BstDSI CCRYGG 1 cut(s) 65
BstENI CCTNNNNNAGG 3 cut(s) 341, 1015, 1990
BstF5I GGATG 9 cut(s) 10, 61, 283, 610, 628, 784, 1850, 2263, 2287
BstHHI GCGC 3 cut(s) 583, 1104, 1976
BstKTI GATC 7 cut(s) 91, 427, 688, 1144, 1556, 1903, 2214
BstMAI GTCTC 5 cut(s) 1061, 1298, 1527, 1700, 1739
BstMBI GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 8 cut(s) 368, 637, 850, 1062, 1847, 1997, 2012, 2201
BstNI CCWGG 7 cut(s) 143, 1620, 1752, 1764, 1875, 2146, 2165
BstNSI RCATGY 2 cut(s) 470, 1790
BstSCI CCNGG 8 cut(s) 141, 438, 1618, 1750, 1762, 1873, 2144, 2163
BstSFI CTRYAG 2 cut(s) 1458, 1650
BstV1I GCAGC 8 cut(s) 202, 277, 595, 1472, 2074, 2096, 2176, 2179
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 1 cut(s) 1553
BstYI RGATCY 1 cut(s) 1553
BstZI CGGCCG 1 cut(s) 353
BsuRI GGCC 8 cut(s) 73, 128, 355, 640, 844, 914, 1872, 2271
BtgI CCRYGG 1 cut(s) 65
BtrI CACGTC 1 cut(s) 489
BtsCI GGATG 9 cut(s) 10, 61, 283, 610, 628, 784, 1850, 2263, 2287
BtsI GCAGTG 2 cut(s) 1111, 1708
BtsIMutI CAGTG 3 cut(s) 1111, 1209, 1708
BveI ACCTGC 2 cut(s) 1668, 1855
Cac8I GCNNGC 6 cut(s) 75, 270, 941, 1737, 1788, 1998
CaiI CAGNNNCTG 3 cut(s) 593, 1763, 2233
CfoI GCGC 3 cut(s) 583, 1104, 1976
Cfr10I RCCGGY 1 cut(s) 1082
Cfr13I GGNCC 6 cut(s) 638, 842, 912, 1275, 1871, 1878
CseI GACGC 2 cut(s) 107, 2193
CsiI ACCWGGT 1 cut(s) 1750
Csp6I GTAC 4 cut(s) 987, 1041, 1180, 2183
CspCI CAANNNNNGTGG 2 cut(s) 1315, 1350
CviAII CATG 6 cut(s) 17, 467, 872, 960, 1126, 1787
CviQI GTAC 4 cut(s) 987, 1041, 1180, 2183
DpnI GATC 7 cut(s) 90, 426, 687, 1143, 1555, 1902, 2213
DpnII GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
EaeI YGGCCR 2 cut(s) 353, 2269
EagI CGGCCG 1 cut(s) 353
Eam1104I CTCTTC 6 cut(s) 324, 512, 746, 1391, 1589, 1691
EarI CTCTTC 6 cut(s) 324, 512, 746, 1391, 1589, 1691
EciI GGCGGA 1 cut(s) 809
EclXI CGGCCG 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 634
Eco31I GGTCTC 1 cut(s) 1061
Eco32I GATATC 1 cut(s) 1353
Eco47I GGWCC 2 cut(s) 1275, 1878
Eco52I CGGCCG 1 cut(s) 353
Eco57I CTGAAG 2 cut(s) 485, 1056
EcoNI CCTNNNNNAGG 3 cut(s) 341, 1015, 1990
EcoO109I RGGNCCY 1 cut(s) 1275
EcoRII CCWGG 7 cut(s) 141, 1618, 1750, 1762, 1873, 2144, 2163
EcoRV GATATC 1 cut(s) 1353
EcoT14I CCWWGG 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 634
Esp3I CGTCTC 1 cut(s) 1298
FaeI CATG 6 cut(s) 20, 470, 875, 963, 1129, 1790
FaqI GGGAC 7 cut(s) 27, 173, 319, 611, 1171, 1261, 2341
FatI CATG 6 cut(s) 16, 466, 871, 959, 1125, 1786
FauI CCCGC 2 cut(s) 636, 2194
FbaI TGATCA 2 cut(s) 685, 1141
Fnu4HI GCNGC 8 cut(s) 191, 266, 584, 1461, 2085, 2088, 2190, 2193
FokI GGATG 9 cut(s) 17, 48, 290, 617, 635, 791, 1837, 2270, 2294
Fsp4HI GCNGC 8 cut(s) 191, 266, 584, 1461, 2085, 2088, 2190, 2193
FspBI CTAG 5 cut(s) 492, 978, 1226, 1677, 1997
FspI TGCGCA 1 cut(s) 1103
GlaI GCGC 3 cut(s) 582, 1103, 1975
GluI GCNGC 8 cut(s) 191, 266, 584, 1461, 2085, 2088, 2190, 2193
GsuI CTGGAG 4 cut(s) 631, 1467, 2255, 2321
HaeIII GGCC 8 cut(s) 73, 128, 355, 640, 844, 914, 1872, 2271
HapII CCGG 6 cut(s) 372, 440, 900, 1083, 1802, 2068
HgaI GACGC 2 cut(s) 107, 2193
HhaI GCGC 3 cut(s) 583, 1104, 1976
Hin1I GRCGYC 1 cut(s) 2204
Hin1II CATG 6 cut(s) 20, 470, 875, 963, 1129, 1790
Hin6I GCGC 3 cut(s) 581, 1102, 1974
HinP1I GCGC 3 cut(s) 581, 1102, 1974
HindIII AAGCTT 2 cut(s) 416, 917
HpaII CCGG 6 cut(s) 372, 440, 900, 1083, 1802, 2068
HphI GGTGA 6 cut(s) 44, 887, 1097, 1136, 1836, 2210
Hpy166II GTNNAC 3 cut(s) 486, 1115, 2105
Hpy8I GTNNAC 3 cut(s) 486, 1115, 2105
Hpy99I CGWCG 3 cut(s) 578, 1835, 2209
HpyCH4III ACNGT 4 cut(s) 201, 986, 2039, 2332
HpyCH4IV ACGT 4 cut(s) 488, 1307, 1437, 2058
HpyF10VI GCNNNNNNNGC 8 cut(s) 368, 637, 850, 1062, 1847, 1997, 2012, 2201
HpySE526I ACGT 4 cut(s) 488, 1307, 1437, 2058
Hsp92I GRCGYC 1 cut(s) 2204
Hsp92II CATG 6 cut(s) 20, 470, 875, 963, 1129, 1790
HspAI GCGC 3 cut(s) 581, 1102, 1974
KflI GGGWCCC 1 cut(s) 1275
Kpn2I TCCGGA 2 cut(s) 899, 1801
KpnI GGTACC 1 cut(s) 2186
Ksp22I TGATCA 2 cut(s) 685, 1141
Kzo9I GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
LguI GCTCTTC 3 cut(s) 746, 1391, 1691
LmnI GCTCC 5 cut(s) 809, 1020, 1173, 2048, 2134
Lsp1109I GCAGC 8 cut(s) 202, 277, 595, 1472, 2074, 2096, 2176, 2179
LweI GCATC 3 cut(s) 1275, 1859, 1993
MabI ACCWGGT 1 cut(s) 1750
MaeI CTAG 5 cut(s) 492, 978, 1226, 1677, 1997
MaeII ACGT 4 cut(s) 488, 1307, 1437, 2058
MaeIII GTNAC 5 cut(s) 980, 1308, 1433, 1861, 2284
MalI GATC 7 cut(s) 90, 426, 687, 1143, 1555, 1902, 2213
MbiI CCGCTC 1 cut(s) 1455
MboI GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
MflI RGATCY 1 cut(s) 1553
MhlI GDGCHC 1 cut(s) 941
MlsI TGGCCA 1 cut(s) 2271
MluNI TGGCCA 1 cut(s) 2271
MlyI GAGTC 6 cut(s) 132, 341, 1231, 1316, 1458, 1529
MmeI TCCRAC 2 cut(s) 265, 2079
Mox20I TGGCCA 1 cut(s) 2271
MroI TCCGGA 2 cut(s) 899, 1801
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 1 cut(s) 2271
MseI TTAA 7 cut(s) 54, 255, 378, 1013, 1467, 1509, 2219
MslI CAYNNNNRTG 1 cut(s) 944
Msp20I TGGCCA 1 cut(s) 2271
MspA1I CMGCKG 5 cut(s) 193, 505, 1463, 2087, 2189
MspI CCGG 6 cut(s) 372, 440, 900, 1083, 1802, 2068
MspR9I CCNGG 8 cut(s) 143, 440, 1620, 1752, 1764, 1875, 2146, 2165
Mva1269I GAATGC 1 cut(s) 514
MvaI CCWGG 7 cut(s) 143, 1620, 1752, 1764, 1875, 2146, 2165
MwoI GCNNNNNNNGC 8 cut(s) 368, 637, 850, 1062, 1847, 1997, 2012, 2201
NciI CCSGG 1 cut(s) 440
NdeII GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
NheI GCTAGC 1 cut(s) 1996
NlaIII CATG 6 cut(s) 20, 470, 875, 963, 1129, 1790
NlaIV GGNNCC 6 cut(s) 1009, 1276, 1277, 1555, 1879, 2184
NmuCI GTSAC 3 cut(s) 980, 1861, 2284
NsbI TGCGCA 1 cut(s) 1103
NspI RCATGY 2 cut(s) 470, 1790
NspV TTCGAA 2 cut(s) 1370, 2093
PaeI GCATGC 1 cut(s) 1790
PaqCI CACCTGC 1 cut(s) 1855
PasI CCCWGGG 1 cut(s) 1874
PciI ACATGT 1 cut(s) 466
PciSI GCTCTTC 3 cut(s) 746, 1391, 1691
PcsI WCGNNNNNNNCGW 2 cut(s) 2285, 2336
PctI GAATGC 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 7 cut(s) 868, 896, 1241, 1291, 1610, 1673, 2261
Pfl23II CGTACG 1 cut(s) 986
PflFI GACNNNGTC 1 cut(s) 1711
PflMI CCANNNNNTGG 1 cut(s) 593
PkrI GCNGC 8 cut(s) 192, 267, 585, 1462, 2086, 2089, 2191, 2194
PleI GAGTC 6 cut(s) 132, 340, 1230, 1316, 1457, 1529
PpsI GAGTC 6 cut(s) 132, 340, 1230, 1316, 1457, 1529
PpuMI RGGWCCY 1 cut(s) 1275
PscI ACATGT 1 cut(s) 466
PsiI TTATAA 1 cut(s) 1257
Psp5II RGGWCCY 1 cut(s) 1275
Psp6I CCWGG 7 cut(s) 141, 1618, 1750, 1762, 1873, 2144, 2163
PspGI CCWGG 7 cut(s) 141, 1618, 1750, 1762, 1873, 2144, 2163
PspLI CGTACG 1 cut(s) 986
PspN4I GGNNCC 6 cut(s) 1009, 1276, 1277, 1555, 1879, 2184
PspPI GGNCC 6 cut(s) 638, 842, 912, 1275, 1871, 1878
PspPPI RGGWCCY 1 cut(s) 1275
PstI CTGCAG 2 cut(s) 1462, 1654
PstNI CAGNNNCTG 3 cut(s) 593, 1763, 2233
PsuI RGATCY 1 cut(s) 1553
PsyI GACNNNGTC 1 cut(s) 1711
PvuII CAGCTG 4 cut(s) 193, 505, 1463, 2087
RsaI GTAC 4 cut(s) 988, 1042, 1181, 2184
RsaNI GTAC 4 cut(s) 987, 1041, 1180, 2183
RseI CAYNNNNRTG 1 cut(s) 944
SapI GCTCTTC 3 cut(s) 746, 1391, 1691
SaqAI TTAA 7 cut(s) 54, 255, 378, 1013, 1467, 1509, 2219
SatI GCNGC 8 cut(s) 191, 266, 584, 1461, 2085, 2088, 2190, 2193
Sau3AI GATC 7 cut(s) 88, 424, 685, 1141, 1553, 1900, 2211
Sau96I GGNCC 6 cut(s) 638, 842, 912, 1275, 1871, 1878
ScaI AGTACT 1 cut(s) 1181
SchI GAGTC 6 cut(s) 132, 341, 1231, 1316, 1458, 1529
ScrFI CCNGG 8 cut(s) 143, 440, 1620, 1752, 1764, 1875, 2146, 2165
SduI GDGCHC 1 cut(s) 941
SexAI ACCWGGT 1 cut(s) 1750
SfaNI GCATC 3 cut(s) 1275, 1859, 1993
SfcI CTRYAG 2 cut(s) 1458, 1650
SfuI TTCGAA 2 cut(s) 1370, 2093
SinI GGWCC 2 cut(s) 1275, 1878
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 3 cut(s) 599, 711, 1363
SmoI CTYRAG 3 cut(s) 599, 711, 1363
SphI GCATGC 1 cut(s) 1790
SsiI CCGC 7 cut(s) 617, 629, 794, 853, 1110, 1453, 2187
SspMI CTAG 5 cut(s) 492, 978, 1226, 1677, 1997
StyD4I CCNGG 8 cut(s) 141, 438, 1618, 1750, 1762, 1873, 2144, 2163
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 4 cut(s) 201, 986, 2039, 2332
TaiI ACGT 4 cut(s) 491, 1310, 1440, 2061
TaqI TCGA 7 cut(s) 242, 576, 1370, 1538, 2093, 2214, 2294
TaqII GACCGA 2 cut(s) 1653, 1844
TatI WGTACW 1 cut(s) 1179
TfiI GAWTC 7 cut(s) 868, 896, 1241, 1291, 1610, 1673, 2261
Tru1I TTAA 7 cut(s) 54, 255, 378, 1013, 1467, 1509, 2219
Tru9I TTAA 7 cut(s) 54, 255, 378, 1013, 1467, 1509, 2219
TscAI CASTG 3 cut(s) 1111, 1216, 1708
TseFI GTSAC 3 cut(s) 980, 1861, 2284
TseI GCWGC 8 cut(s) 190, 265, 583, 1460, 2084, 2087, 2189, 2192
Tsp45I GTSAC 3 cut(s) 980, 1861, 2284
TspDTI ATGAA 6 cut(s) 174, 962, 1142, 1186, 1427, 2063
TspGWI ACGGA 4 cut(s) 54, 177, 1004, 2031
TspRI CASTG 3 cut(s) 1111, 1216, 1708
Tth111I GACNNNGTC 1 cut(s) 1711
Van91I CCANNNNNTGG 1 cut(s) 593
VpaK11BI GGWCC 2 cut(s) 1275, 1878
XagI CCTNNNNNAGG 3 cut(s) 341, 1015, 1990
XapI RAATTY 2 cut(s) 81, 703
XbaI TCTAGA 1 cut(s) 1225
XceI RCATGY 2 cut(s) 470, 1790
XcmI CCANNNNNNNNNTGG 1 cut(s) 648
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 5 cut(s) 492, 978, 1226, 1677, 1997
ZrmI AGTACT 1 cut(s) 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.