Rw2G052440

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002210
Physical Location & Seq
Reverse (-)
80386601 .. 80390402
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052440.1

Sequence Viewer

Length: 3249 bp
ATGGGGACGTTGGGGAGATCTACAGCCGTAGCTGATGGTTGCTCGGGTGGCTCCGGGTCGGGTTCTGGACCGGCGAGGTTTGAGGCCGGGGAGGAGAGGAGGAGGATTGACGACTCGGGTGGGGGCCGGGTCGGGTCGGGAGGGTCCGGAGGGGAGCTGGTGAGGTCGTTCGTTGAGCTGGTTAAGACTGTGGAGGCTAAAGTCTCGGTTGGGATGAGTTTTGGGGACATGGATACGTGTATGTCGAGGTTGAGAGGGAGTGAGAGCGTTGGAGAGCTGGAGTCTGGTTTGGGTGAGGAGAGGAGAGTAGTGAGCATTGGTTCGAAGACGAATTCGGATGCGAATGTAGGGGGGGATGAGGACAATGGGAATTGTTGTGATGAAACCAAGGCCAACGGTGTTGGGGTTGAGGAGAATGGCAAGTGTTTGGATGAAATTGTGGAAGAGCTAGATGGGGGAATTGAGGTTCCCATTGCCGGTACGAGTGAAAATGGTGAGGACCGGGTGGGAGAAGAGTTGAGTGATGGAGTGAATGAGTTCTGTATCGGAGATTTTGTGTGGGGTAAGATTAAGAACCACCCGTGGTGGCCGGGCCAGATTTGTGATCCTTCGGATGCTTCTGAGTTTGCAGTGAAGTTGAGATCCAAGGACAAGCTTCTTGTAGCGTACTTTGGGGATGACACCTTCGCGTGGTGCCATCCGTCGCAATTGAAACCCTTTGAGGAAAATTTCGTGGAGAATTCTAAGCTGAGTAGCTCCAAGGTTTTTGTTATTGCTGTGCAGCAGGCTGTGGATGAGATAGGTAGGCTTGTAAAGTTGAAGATGAGTTGCGCTTGCATGGGGGAAGAGTTTCTAAGTGGTCGTAAACAGCCTTTAGCATCGAATGCTGGAGTTAAGGAAGGAGTTGTTGTCCCTGAAGGCCGAGTTGGGAAGTTTTTGGGTCATCTATCTCAGTCGGTTGACCTTCTTGCGGAATTGAAACATATGGCACAAGCTGTATCCTTGACTAGTGTTCTTGAGCTCAATGTATTAAAGAGTTTGCTGTCTGCCTTTTATTGTTCGAAAGGGGGATATCAGTTACCTGTTTACATTGAACCCCAGCTAATTCCTGGTCTTGAAGATGAGCGGAAGATGGTGGAAGTCCCAGTGCAGTGGCCATCTGATGACAGGTCGTCTTCTCCCATAGGCTCCAATACTGGAAACACTGAACAAAATTTGTTGCAAAGGTCACCACCAAGTATAGAAAATAGACAGCCCCATAAAAGGAAGGAGAAGACCATTGCTGATCTTCTTGGAGAAGCCAATCGTGTTGAGGTAAAAAGTATGGATGGGGTTGTAGCTAAAGAAAGTGCAATCTCAGAGAAACGAGGGCTATCATCCAGACGGAAGAAGCAGAAAAGCAGTGATATTCATGGTAAGGGTAGATTGACTTTCGAATCAGGAAGGGAAAGAGCCAAACTCTCAAAATCGCCCAGGAGAAAGGAAGAAGATGAAGGTATTGGCATCGAGAGTAGTGATGGTTTGGCTAAAGAGAGTGCTGGTGGAAATGAAGAAACTAAGAAGACTCCTCTCTCAAGGAAAAGGAAGAGGGATGAAGATAGCATCGACAGTAAAGATGGTGATACCCAAGAAAAAAGTGGTGAAAAGGATGAAACTAAGAAGGCTCCGTTGTCAAGGAGAAGGAAGAAGAATGAAGATATTCACATCAACAGTAATGATGGTGAGGCCAAAGGAAAAGCTGGTGCAAGAGTGGGGGAGTTGCTCAATGTTGGTTTACAAACAGATATGAAAGACCAAATTGGTAAGAGCCCTTTGTTGAGGGAAAGAAAAAAAAGCAAGTACTTGTCCCCACCCTTCACAATAAGTGACGCAGGGAAAAGAAAACGAAACTTGGAAACAGAATCTCGAAATGCTGTAACGAGTCATGATTTGGGGAAAGAGGGGAAAGAGCAAGACATGGAATCAGTATTATCTCTGGAGGTTTCTAGTAAAGCTCAGCTTGGGGACAGGATTACCAGGGATGCTGCCAACATTAAGGGGTCCCCTCAGATTTTGAATAGTTGCAGTGAGGCATTACAAGAGAAACTCTCAATAGAAGGTGGGAATAAGAGCACTGATGTTAAAACACCAGCAGAAGGTGAGAAGAAGCGTGTCGATCCAATAAAAGCTAATGCAGCCACTAATGAAGTGCTGTCTGGAGTCCGCTCTGCAGCTCTTGATCCATTATATCCAGTCAAAAGGAAATCCTCCTTTGAGATTGTTGTTGATTTTGTGTCCATATTTAGAGACTCAGTTTACCACAATGGCTGCTACCACAAGGTTTACAAGAAGCACCAACCTCATGGAAAGAGGAAGAGATCAGATTCTGAACTGGGGTCATTGGTGAAAGACAATCGAACTGCTGCAAATTTGCATGAAGATGAACCTGGTAACAGGGTTATGAAGAAGAAACAAGATATGAAGTCTGATAAGCCTACAAAAAAGCAAGCTGGTGAGACACGAAGTTCAAAGAGAAGGAAGTTAAATTCTGTAATTAAGTCGTTGGGAAAAGACAATGAGAAAGCAGAAAATCAGCCTGTCAGTGAATCTGGGGAGAGGAACATCAACACTAATCAAGATAAGCCTAAGAAAAAGCAAGCTGCTGAGCCACCAATTTCAAAGCTGGTCAGGCGGAGATCAAAGCATGCTGACACTCGGGATTTGAAGATTAAGGAGACGGAAGAGGAGGCTCCGCCTACATTCCTTTTTGTAACATTTGGACCTGGATCCTCGCTGCCTACCAAGGATGATCTTCTCAGATTATACAGTAAATTTGGGGAACTAAATGAAACTGAAACTGAGATGTTCTACACCAATTTCTGTGCTCGTGTTGCGTTTGTAATGAGCTCTGACGCGGAAGAAGCCTTCAATCATTCTCTAATTGACAGTCCCTTTGGAGCATCCAATGTAAATTTCCGCCTCCAAAATCCCTCAGGTGCTTCCAAGACTCGTGAGCTCAGTGCAATACCCGAGTCTCCTCCAGCTAAGAAGAAGTCCAGGGGCAAGACTGCAAGTGAGGCATTAGTAGCTTCACAACCACCTGCTGGTGAGCCCTCGCAAGTTGATTTCATTAGGCATAGACTTAAGATGATGAGCTCAATGCTGGGAAACTCAGATCGCAAAGTGTCGCCTGTTGCAACATCGAAAATTCAGAATGAGATAAAGGAGCTACTGAAGGTCGTAAGCACAATGGGTGAATCCTCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1082

Amino Acids

118.08

Weight (kDa)

8.51

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 183 - 266 3.1e-20 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3088
Acc36I ACCTGC 1 cut(s) 3088
AccB1I GGYRCC 1 cut(s) 693
AccB7I CCANNNNNTGG 1 cut(s) 3083
AccBSI CCGCTC 2 cut(s) 1126, 2205
AccII CGCG 2 cut(s) 689, 2894
AccIII TCCGGA 1 cut(s) 146
AciI CCGC 7 cut(s) 971, 1126, 2203, 2671, 2732, 2894, 2956
AclWI GGATC 6 cut(s) 599, 636, 2150, 2213, 2760, 2773
AcoI YGGCCR 2 cut(s) 587, 1154
AcsI RAATTY 9 cut(s) 331, 727, 739, 1213, 2407, 2524, 2810, 2950, 3186
AcuI CTGAAG 2 cut(s) 936, 3233
AfaI GTAC 3 cut(s) 481, 668, 1841
AfiI CCNNNNNNNGG 6 cut(s) 476, 690, 970, 1263, 2135, 3083
AflII CTTAAG 1 cut(s) 3122
AflIII ACRYGT 1 cut(s) 236
AhdI GACNNNNNGTC 1 cut(s) 1171
AhlI ACTAGT 1 cut(s) 1007
AjnI CCWGG 6 cut(s) 1108, 1472, 2015, 2425, 2761, 3035
AjuI GAANNNNNNNTTGG 4 cut(s) 909, 941, 2936, 2968
AloI GAACNNNNNNTCC 2 cut(s) 450, 482
Alw21I GWGCWC 6 cut(s) 1023, 2114, 2866, 2888, 2997, 3137
Alw26I GTCTC 5 cut(s) 208, 2280, 2489, 2708, 3018
AlwI GGATC 6 cut(s) 599, 636, 2150, 2213, 2760, 2773
AlwNI CAGNNNCTG 1 cut(s) 2366
Ama87I CYCGRG 4 cut(s) 43, 115, 2694, 3008
Aor13HI TCCGGA 1 cut(s) 146
AoxI GGCC 8 cut(s) 84, 124, 390, 587, 592, 919, 1154, 1725
ApeKI GCWGC 8 cut(s) 781, 2024, 2174, 2210, 2307, 2402, 2639, 2773
ApoI RAATTY 9 cut(s) 331, 727, 739, 1213, 2407, 2524, 2810, 2950, 3186
ArsI GACNNNNNNTTYG 2 cut(s) 2915, 2947
Asp700I GAANNNNTTC 3 cut(s) 536, 849, 1698
AspLEI GCGC 1 cut(s) 833
AspS9I GGNCC 7 cut(s) 68, 124, 144, 499, 592, 2040, 2759
AsuC2I CCSGG 5 cut(s) 55, 88, 128, 503, 591
AsuII TTCGAA 3 cut(s) 323, 1061, 1434
AvaI CYCGRG 4 cut(s) 43, 115, 2694, 3008
AvaII GGWCC 5 cut(s) 68, 144, 499, 2040, 2759
AxyI CCTNAGG 1 cut(s) 2971
BalI TGGCCA 1 cut(s) 1156
BamHI GGATCC 1 cut(s) 2765
BanI GGYRCC 1 cut(s) 693
BanII GRGCYC 6 cut(s) 1023, 1811, 2888, 2997, 3093, 3137
BauI CACGAG 2 cut(s) 2865, 2988
BbsI GAAGAC 4 cut(s) 332, 1167, 1280, 1568
Bbv12I GWGCWC 6 cut(s) 1023, 2114, 2866, 2888, 2997, 3137
BbvI GCAGC 8 cut(s) 793, 2011, 2186, 2222, 2294, 2389, 2626, 2760
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 6 cut(s) 1110, 1474, 2017, 2427, 2763, 3037
BciVI GTATCC 2 cut(s) 226, 1009
BcnI CCSGG 5 cut(s) 55, 88, 128, 503, 591
BcoDI GTCTC 5 cut(s) 208, 2280, 2489, 2708, 3018
BcuI ACTAGT 1 cut(s) 1007
BfaI CTAG 3 cut(s) 449, 1008, 1986
BfmI CTRYAG 2 cut(s) 21, 2208
BfrI CTTAAG 1 cut(s) 3122
BfuAI ACCTGC 1 cut(s) 3088
BfuI GTATCC 2 cut(s) 226, 1009
BglII AGATCT 1 cut(s) 17
BisI GCNGC 8 cut(s) 782, 2025, 2175, 2211, 2308, 2403, 2640, 2774
BlpI GCTNAGC 2 cut(s) 1995, 2643
BlsI GCNGC 8 cut(s) 783, 2026, 2176, 2212, 2309, 2404, 2641, 2775
BmcAI AGTACT 1 cut(s) 1841
Bme18I GGWCC 5 cut(s) 68, 144, 499, 2040, 2759
BmeRI GACNNNNNGTC 1 cut(s) 1171
BmeT110I CYCGRG 4 cut(s) 43, 115, 2694, 3008
BmgT120I GGNCC 7 cut(s) 68, 124, 144, 499, 592, 2040, 2759
BmrI ACTGGG 2 cut(s) 1139, 2381
BmsI GCATC 7 cut(s) 328, 604, 887, 1512, 1611, 2011, 2948
BmuI ACTGGG 2 cut(s) 1139, 2381
BpiI GAAGAC 4 cut(s) 332, 1167, 1280, 1568
BplI GAGNNNNNCTC 4 cut(s) 1443, 1475, 2072, 2104
BpmI CTGGAG 5 cut(s) 299, 909, 1997, 2217, 3003
Bpu1102I GCTNAGC 2 cut(s) 1995, 2643
Bpu14I TTCGAA 3 cut(s) 323, 1061, 1434
BpuEI CTTGAG 2 cut(s) 1037, 1558
BpuMI CCSGG 5 cut(s) 55, 88, 128, 503, 591
BsaAI YACGTR 1 cut(s) 237
BsaJI CCNNGG 9 cut(s) 87, 387, 581, 645, 759, 1472, 2016, 2781, 3036
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 4 cut(s) 540, 570, 894, 924
Bsc4I CCNNNNNNNGG 6 cut(s) 476, 690, 970, 1263, 2135, 3083
Bse118I RCCGGY 2 cut(s) 70, 476
Bse1I ACTGG 4 cut(s) 1145, 1201, 2231, 2376
Bse21I CCTNAGG 1 cut(s) 2971
Bse3DI GCAATG 2 cut(s) 471, 1278
BseAI TCCGGA 1 cut(s) 146
BseBI CCWGG 6 cut(s) 1110, 1474, 2017, 2427, 2763, 3037
BseDI CCNNGG 9 cut(s) 87, 387, 581, 645, 759, 1472, 2016, 2781, 3036
BseLI CCNNNNNNNGG 6 cut(s) 476, 690, 970, 1263, 2135, 3083
BseMI GCAATG 2 cut(s) 471, 1278
BseNI ACTGG 4 cut(s) 1145, 1201, 2231, 2376
BseRI GAGGAG 9 cut(s) 107, 112, 115, 311, 316, 425, 1557, 2738, 3006
BseXI GCAGC 8 cut(s) 793, 2011, 2186, 2222, 2294, 2389, 2626, 2760
BseYI CCCAGC 2 cut(s) 1098, 3142
BsgI GTGCAG 2 cut(s) 800, 1169
Bsh1236I CGCG 2 cut(s) 689, 2894
BshFI GGCC 8 cut(s) 86, 126, 392, 589, 594, 921, 1156, 1727
BshNI GGYRCC 1 cut(s) 693
BsiHKAI GWGCWC 6 cut(s) 1023, 2114, 2866, 2888, 2997, 3137
BsiHKCI CYCGRG 4 cut(s) 43, 115, 2694, 3008
BsiSI CCGG 8 cut(s) 54, 71, 87, 127, 147, 477, 502, 590
BslFI GGGAC 8 cut(s) 19, 239, 896, 1127, 1831, 2018, 2026, 2913
BslI CCNNNNNNNGG 6 cut(s) 476, 690, 970, 1263, 2135, 3083
BsmAI GTCTC 5 cut(s) 208, 2280, 2489, 2708, 3018
BsmBI CGTCTC 1 cut(s) 2708
BsmFI GGGAC 8 cut(s) 19, 239, 896, 1127, 1831, 2018, 2026, 2913
BsmI GAATGC 1 cut(s) 889
BsnI GGCC 8 cut(s) 86, 126, 392, 589, 594, 921, 1156, 1727
BsoBI CYCGRG 4 cut(s) 43, 115, 2694, 3008
Bsp119I TTCGAA 3 cut(s) 323, 1061, 1434
Bsp1286I GDGCHC 8 cut(s) 1023, 1811, 2114, 2866, 2888, 2997, 3093, 3137
Bsp13I TCCGGA 1 cut(s) 146
Bsp1720I GCTNAGC 2 cut(s) 1995, 2643
BspACI CCGC 7 cut(s) 971, 1126, 2203, 2671, 2732, 2894, 2956
BspANI GGCC 8 cut(s) 86, 126, 392, 589, 594, 921, 1156, 1727
BspEI TCCGGA 1 cut(s) 146
BspFNI CGCG 2 cut(s) 689, 2894
BspHI TCATGA 1 cut(s) 1924
BspMAI CTGCAG 1 cut(s) 2212
BspMI ACCTGC 1 cut(s) 3088
BspPI GGATC 6 cut(s) 599, 636, 2150, 2213, 2760, 2773
BspQI GCTCTTC 1 cut(s) 438
BspT104I TTCGAA 3 cut(s) 323, 1061, 1434
BspT107I GGYRCC 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 3122
BsrBI CCGCTC 2 cut(s) 1126, 2205
BsrDI GCAATG 2 cut(s) 471, 1278
BsrFI RCCGGY 2 cut(s) 70, 476
BsrI ACTGG 4 cut(s) 1145, 1201, 2231, 2376
BssAI RCCGGY 2 cut(s) 70, 476
BssECI CCNNGG 9 cut(s) 87, 387, 581, 645, 759, 1472, 2016, 2781, 3036
BssSI CACGAG 2 cut(s) 2865, 2988
BssT1I CCWWGG 4 cut(s) 387, 645, 759, 2781
Bst2BI CACGAG 2 cut(s) 2865, 2988
Bst2UI CCWGG 6 cut(s) 1110, 1474, 2017, 2427, 2763, 3037
Bst4CI ACNGT 6 cut(s) 190, 398, 1610, 1712, 2807, 2927
Bst6I CTCTTC 6 cut(s) 438, 507, 840, 1580, 2348, 2715
BstAFI CTTAAG 1 cut(s) 3122
BstAPI GCANNNNNTGC 1 cut(s) 884
BstBAI YACGTR 1 cut(s) 237
BstBI TTCGAA 3 cut(s) 323, 1061, 1434
BstC8I GCNNGC 5 cut(s) 786, 835, 2487, 2637, 2685
BstDSI CCRYGG 1 cut(s) 581
BstEII GGTNACC 1 cut(s) 1227
BstFNI CGCG 2 cut(s) 689, 2894
BstHHI GCGC 1 cut(s) 833
BstMAI GTCTC 5 cut(s) 208, 2280, 2489, 2708, 3018
BstMWI GCNNNNNNNGC 8 cut(s) 48, 884, 2174, 2668, 2870, 2900, 3056, 3065
BstNI CCWGG 6 cut(s) 1110, 1474, 2017, 2427, 2763, 3037
BstNSI RCATGY 1 cut(s) 2687
BstPI GGTNACC 1 cut(s) 1227
BstSFI CTRYAG 2 cut(s) 21, 2208
BstUI CGCG 2 cut(s) 689, 2894
BstV1I GCAGC 8 cut(s) 793, 2011, 2186, 2222, 2294, 2389, 2626, 2760
BstV2I GAAGAC 4 cut(s) 332, 1167, 1280, 1568
BstX2I RGATCY 3 cut(s) 17, 641, 2765
BstXI CCANNNNNNTGG 2 cut(s) 1152, 2342
BstYI RGATCY 3 cut(s) 17, 641, 2765
Bsu36I CCTNAGG 1 cut(s) 2971
BsuI GTATCC 2 cut(s) 226, 1009
BsuRI GGCC 8 cut(s) 86, 126, 392, 589, 594, 921, 1156, 1727
BtgI CCRYGG 1 cut(s) 581
BtsI GCAGTG 4 cut(s) 636, 1157, 1408, 2071
BtsIMutI CAGTG 9 cut(s) 636, 1152, 1157, 1203, 1408, 2071, 2112, 2587, 3004
BveI ACCTGC 1 cut(s) 3088
Cac8I GCNNGC 5 cut(s) 786, 835, 2487, 2637, 2685
CaiI CAGNNNCTG 1 cut(s) 2366
CciI TCATGA 1 cut(s) 1924
CfoI GCGC 1 cut(s) 833
Cfr10I RCCGGY 2 cut(s) 70, 476
Cfr13I GGNCC 7 cut(s) 68, 124, 144, 499, 592, 2040, 2759
CseI GACGC 2 cut(s) 1877, 2900
CsiI ACCWGGT 1 cut(s) 2425
Csp6I GTAC 3 cut(s) 480, 667, 1840
CviAII CATG 8 cut(s) 229, 838, 1412, 1925, 1957, 2342, 2414, 2684
CviQI GTAC 3 cut(s) 480, 667, 1840
DriI GACNNNNNGTC 1 cut(s) 1171
EaeI YGGCCR 2 cut(s) 587, 1154
Eam1104I CTCTTC 6 cut(s) 438, 507, 840, 1580, 2348, 2715
Eam1105I GACNNNNNGTC 1 cut(s) 1171
EarI CTCTTC 6 cut(s) 438, 507, 840, 1580, 2348, 2715
EciI GGCGGA 3 cut(s) 2686, 2721, 2945
Ecl136II GAGCTC 4 cut(s) 1021, 2886, 2995, 3135
Eco130I CCWWGG 4 cut(s) 387, 645, 759, 2781
Eco24I GRGCYC 6 cut(s) 1023, 1811, 2888, 2997, 3093, 3137
Eco32I GATATC 1 cut(s) 1073
Eco47I GGWCC 5 cut(s) 68, 144, 499, 2040, 2759
Eco53kI GAGCTC 4 cut(s) 1021, 2886, 2995, 3135
Eco57I CTGAAG 2 cut(s) 936, 3233
Eco81I CCTNAGG 1 cut(s) 2971
Eco88I CYCGRG 4 cut(s) 43, 115, 2694, 3008
Eco91I GGTNACC 1 cut(s) 1227
EcoICRI GAGCTC 4 cut(s) 1021, 2886, 2995, 3135
EcoO109I RGGNCCY 1 cut(s) 2040
EcoO65I GGTNACC 1 cut(s) 1227
EcoRI GAATTC 2 cut(s) 331, 739
EcoRII CCWGG 6 cut(s) 1108, 1472, 2015, 2425, 2761, 3035
EcoRV GATATC 1 cut(s) 1073
EcoT14I CCWWGG 4 cut(s) 387, 645, 759, 2781
EcoT38I GRGCYC 6 cut(s) 1023, 1811, 2888, 2997, 3093, 3137
ErhI CCWWGG 4 cut(s) 387, 645, 759, 2781
Esp3I CGTCTC 1 cut(s) 2708
FaeI CATG 8 cut(s) 232, 841, 1415, 1928, 1960, 2345, 2417, 2687
FalI AAGNNNNNCTT 2 cut(s) 1983, 2015
FaqI GGGAC 8 cut(s) 19, 239, 896, 1127, 1831, 2018, 2026, 2913
FatI CATG 8 cut(s) 228, 837, 1411, 1924, 1956, 2341, 2413, 2683
FauNDI CATATG 1 cut(s) 984
Fnu4HI GCNGC 8 cut(s) 782, 2025, 2175, 2211, 2308, 2403, 2640, 2774
FriOI GRGCYC 6 cut(s) 1023, 1811, 2888, 2997, 3093, 3137
Fsp4HI GCNGC 8 cut(s) 782, 2025, 2175, 2211, 2308, 2403, 2640, 2774
FspBI CTAG 3 cut(s) 449, 1008, 1986
GlaI GCGC 1 cut(s) 832
GluI GCNGC 8 cut(s) 782, 2025, 2175, 2211, 2308, 2403, 2640, 2774
GsaI CCCAGC 2 cut(s) 1102, 3146
GsuI CTGGAG 5 cut(s) 299, 909, 1997, 2217, 3003
HaeIII GGCC 8 cut(s) 86, 126, 392, 589, 594, 921, 1156, 1727
HapII CCGG 8 cut(s) 54, 71, 87, 127, 147, 477, 502, 590
HgaI GACGC 2 cut(s) 1877, 2900
HhaI GCGC 1 cut(s) 833
Hin1II CATG 8 cut(s) 232, 841, 1415, 1928, 1960, 2345, 2417, 2687
Hin6I GCGC 1 cut(s) 831
HinP1I GCGC 1 cut(s) 831
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HindIII AAGCTT 1 cut(s) 653
HpaII CCGG 8 cut(s) 54, 71, 87, 127, 147, 477, 502, 590
Hpy166II GTNNAC 6 cut(s) 866, 961, 1087, 1775, 2296, 2323
Hpy8I GTNNAC 6 cut(s) 866, 961, 1087, 1775, 2296, 2323
Hpy99I CGWCG 1 cut(s) 706
HpyCH4III ACNGT 6 cut(s) 190, 398, 1610, 1712, 2807, 2927
HpyCH4IV ACGT 2 cut(s) 8, 236
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 884, 2174, 2668, 2870, 2900, 3056, 3065
HpySE526I ACGT 2 cut(s) 8, 236
Hsp92II CATG 8 cut(s) 232, 841, 1415, 1928, 1960, 2345, 2417, 2687
HspAI GCGC 1 cut(s) 831
KflI GGGWCCC 1 cut(s) 2040
Kpn2I TCCGGA 1 cut(s) 146
LguI GCTCTTC 1 cut(s) 438
LmnI GCTCC 8 cut(s) 56, 154, 761, 1193, 1669, 2734, 2936, 3205
Lsp1109I GCAGC 8 cut(s) 793, 2011, 2186, 2222, 2294, 2389, 2626, 2760
LweI GCATC 7 cut(s) 328, 604, 887, 1512, 1611, 2011, 2948
MabI ACCWGGT 1 cut(s) 2425
MaeI CTAG 3 cut(s) 449, 1008, 1986
MaeII ACGT 2 cut(s) 8, 236
MaeIII GTNAC 6 cut(s) 1077, 1227, 1865, 1915, 2429, 2749
MbiI CCGCTC 2 cut(s) 1126, 2205
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 3 cut(s) 17, 641, 2765
MhlI GDGCHC 8 cut(s) 1023, 1811, 2114, 2866, 2888, 2997, 3093, 3137
MlsI TGGCCA 1 cut(s) 1156
MluNI TGGCCA 1 cut(s) 1156
MlyI GAGTC 8 cut(s) 107, 290, 1558, 1930, 2208, 2282, 2980, 3020
MmeI TCCRAC 1 cut(s) 250
Mox20I TGGCCA 1 cut(s) 1156
MroI TCCGGA 1 cut(s) 146
MroXI GAANNNNTTC 3 cut(s) 536, 849, 1698
MscI TGGCCA 1 cut(s) 1156
MslI CAYNNNNRTG 1 cut(s) 2418
Msp20I TGGCCA 1 cut(s) 1156
MspCI CTTAAG 1 cut(s) 3122
MspI CCGG 8 cut(s) 54, 71, 87, 127, 147, 477, 502, 590
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 889
MvaI CCWGG 6 cut(s) 1110, 1474, 2017, 2427, 2763, 3037
MvnI CGCG 2 cut(s) 689, 2894
MwoI GCNNNNNNNGC 8 cut(s) 48, 884, 2174, 2668, 2870, 2900, 3056, 3065
NciI CCSGG 5 cut(s) 55, 88, 128, 503, 591
NdeI CATATG 1 cut(s) 984
NlaIII CATG 8 cut(s) 232, 841, 1415, 1928, 1960, 2345, 2417, 2687
NmeAIII GCCGAG 1 cut(s) 947
NmuCI GTSAC 2 cut(s) 1227, 1865
NspI RCATGY 1 cut(s) 2687
NspV TTCGAA 3 cut(s) 323, 1061, 1434
PaeI GCATGC 1 cut(s) 2687
PagI TCATGA 1 cut(s) 1924
PaqCI CACCTGC 1 cut(s) 3088
PciSI GCTCTTC 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PctI GAATGC 1 cut(s) 889
PdmI GAANNNNTTC 3 cut(s) 536, 849, 1698
PfeI GAWTC 6 cut(s) 1436, 1901, 1961, 2363, 2585, 3236
PflMI CCANNNNNTGG 1 cut(s) 3083
PkrI GCNGC 8 cut(s) 783, 2026, 2176, 2212, 2309, 2404, 2641, 2775
PleI GAGTC 8 cut(s) 107, 289, 1558, 1929, 2207, 2282, 2980, 3019
PpsI GAGTC 8 cut(s) 107, 289, 1558, 1929, 2207, 2282, 2980, 3019
Ppu21I YACGTR 1 cut(s) 237
PpuMI RGGWCCY 1 cut(s) 2040
Psp124BI GAGCTC 4 cut(s) 1023, 2888, 2997, 3137
Psp5II RGGWCCY 1 cut(s) 2040
Psp6I CCWGG 6 cut(s) 1108, 1472, 2015, 2425, 2761, 3035
PspEI GGTNACC 1 cut(s) 1227
PspFI CCCAGC 2 cut(s) 1098, 3142
PspGI CCWGG 6 cut(s) 1108, 1472, 2015, 2425, 2761, 3035
PspPI GGNCC 7 cut(s) 68, 124, 144, 499, 592, 2040, 2759
PspPPI RGGWCCY 1 cut(s) 2040
PstI CTGCAG 1 cut(s) 2212
PstNI CAGNNNCTG 1 cut(s) 2366
PsuI RGATCY 3 cut(s) 17, 641, 2765
RsaI GTAC 3 cut(s) 481, 668, 1841
RsaNI GTAC 3 cut(s) 480, 667, 1840
RseI CAYNNNNRTG 1 cut(s) 2418
SacI GAGCTC 4 cut(s) 1023, 2888, 2997, 3137
SapI GCTCTTC 1 cut(s) 438
SatI GCNGC 8 cut(s) 782, 2025, 2175, 2211, 2308, 2403, 2640, 2774
Sau96I GGNCC 7 cut(s) 68, 124, 144, 499, 592, 2040, 2759
ScaI AGTACT 1 cut(s) 1841
SchI GAGTC 8 cut(s) 107, 290, 1558, 1930, 2208, 2282, 2980, 3020
SduI GDGCHC 8 cut(s) 1023, 1811, 2114, 2866, 2888, 2997, 3093, 3137
SexAI ACCWGGT 1 cut(s) 2425
SfaNI GCATC 7 cut(s) 328, 604, 887, 1512, 1611, 2011, 2948
SfcI CTRYAG 2 cut(s) 21, 2208
SfuI TTCGAA 3 cut(s) 323, 1061, 1434
SinI GGWCC 5 cut(s) 68, 144, 499, 2040, 2759
SmiMI CAYNNNNRTG 1 cut(s) 2418
SmlI CTYRAG 3 cut(s) 1016, 1573, 3122
SmoI CTYRAG 3 cut(s) 1016, 1573, 3122
SpeI ACTAGT 1 cut(s) 1007
SphI GCATGC 1 cut(s) 2687
SsiI CCGC 7 cut(s) 971, 1126, 2203, 2671, 2732, 2894, 2956
SspMI CTAG 3 cut(s) 449, 1008, 1986
SstI GAGCTC 4 cut(s) 1023, 2888, 2997, 3137
StyI CCWWGG 4 cut(s) 387, 645, 759, 2781
TaaI ACNGT 6 cut(s) 190, 398, 1610, 1712, 2807, 2927
TaiI ACGT 2 cut(s) 11, 239
TatI WGTACW 1 cut(s) 1839
TfiI GAWTC 6 cut(s) 1436, 1901, 1961, 2363, 2585, 3236
TscAI CASTG 9 cut(s) 636, 1152, 1157, 1210, 1408, 2071, 2119, 2587, 3004
TseFI GTSAC 2 cut(s) 1227, 1865
TseI GCWGC 8 cut(s) 781, 2024, 2174, 2210, 2307, 2402, 2639, 2773
Tsp45I GTSAC 2 cut(s) 1227, 1865
TspGWI ACGGA 4 cut(s) 690, 1399, 1656, 2732
TspRI CASTG 9 cut(s) 636, 1152, 1157, 1210, 1408, 2071, 2119, 2587, 3004
Van91I CCANNNNNTGG 1 cut(s) 3083
Vha464I CTTAAG 1 cut(s) 3122
VpaK11BI GGWCC 5 cut(s) 68, 144, 499, 2040, 2759
XapI RAATTY 9 cut(s) 331, 727, 739, 1213, 2407, 2524, 2810, 2950, 3186
XceI RCATGY 1 cut(s) 2687
XcmI CCANNNNNNNNNTGG 2 cut(s) 1106, 1634
XmnI GAANNNNTTC 3 cut(s) 536, 849, 1698
XspI CTAG 3 cut(s) 449, 1008, 1986
ZrmI AGTACT 1 cut(s) 1841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.