Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Reverse (-)

19905591 .. 19910244

Gene Structure 4654 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G53720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGCCCTTCAACATAACCTCCGTGAAAACCTCATCTAACGGAGTATGGCAAGGCGACAATCCTTTAAACTTCGCTTTTCCGTTACTCATCGTCCAAACGGCGTTAATCATCGCCGTCAGTCGCTTCCTCGCCGTCTTATTCAAACCTCTCCGTCAACCCAAAGTCATCGCCGAGATTGTCGGAGGGATTTTGTTAGGACCATCGGCTTTAGGTAGAAACATGGCGTACATGGACCGTATATTTCCGAAATGGAGTATGCCGATACTCGAATCCGTCGCGAGCATAGGACTTCTCTTCTTCCTCTTCCTCGTCGGTCTAGAACTCGATTTATCATCGATCCGACGAAGCGGCAAACGCGCTTTCGGAATCGCAGTCGCTGGAATTACACTACCGTTTATCGCCGGCGTCGGAGTCGCGTTTGTGATCCGTAACACTCTCTACACCGCCGCGGATAAACCAGGTTACGCCGAGTTTCTCGTTTTCATGGGAGTCGCACTCTCGATCACAGCTTTTCCGGTACTTGCGCGTATTTTAGCAGAGCTCAAGCTTTTAACGACTCAGATAGGAGAAACCGCGATGGCTGCAGCCGCTTTTAACGATGTAGCCGCGTGGATTTTACTCGCTTTAGCGGTTGCGTTAGCGGGTAATGGCGGTGAGGGAGGTGGAGAGAAAAAGAGTCCGTTAGTGTCGTTGTGGGTTTTGTTATCGGGCGCTGGGTTTGTGGTTTTTATGTTGGTTGTGATCCGACCCGGAATGAAATGGGTCGCGAAACGTGGATCTCCTGAAAACGACGTCGTACGCGAGTCTTACGTGTGTTTGACGTTAGCCGGTGTTATGGTTTCCGGTTTCGCGACGGATTTAATTGGGATTCATTCGATTTTTGGAGCGTTTGTTTTCGGTTTGACTATACCGAAAGATGGAGAGTTTGGTCAGCGATTGATTGAACGAATTGAGGATTTTGTTTCCGGTTTACTCTTACCGCTTTATTTCGCTACGAGTGGTTTGAAGACTGACGTGGCTAAGATTAGAGGAGCTGAGTCGTGGGGGATGTTGGGTCTTGTTGTTGTTACGGCTTGTGCCGGGAAGATAGTCGGAACTTTTGTTGTGGCGGTGATGGTTAAAGTTCCGGCGAGAGAGGCGTTGACACTTGGTTTCTTGATGAATACTAAAGGTTTAGTGGAGCTCATTGTACTCAACATAGGCAAGGAGAAAAAGGTACTAAACGACGAGACGTTTGCAATACTAGTGCTAATGGCACTCTTCACAACGTTCATAACGACGCCTACTGTAATGGCCATTTACAAGCCGGCACGTGGCACCCACCGCAAACTAAAAGACTTGTCGGCGAGCCAAGACTCCACCAAGGAAGAGCTTCGCATCCTAGCCTGCCTCCACGGCCCAGCCAATGTCTCCTCCCTCATCTCTCTCGTCGAGTCCATCCGAACCACCAAGATACTACGGCTAAAGCTGTTTGTGATGCATCTGATGGAACTAACGGAACGGTCTTCGTCAATCATAATGGTGCAAAGAGCCCGTAAAAACGGACTTCCTTTCGTTCACCGTTACCGTCATGGTGAGCGTCACAGCAACGTCATAGGAGGCTTCGAAGCCTATCGTCAACTAGGCCGGGTCGCAGTCCGGCCCATCACCGCAGTCTCTCCATTACCCACAATGCACGAAGACATTTGCCACATGGCAGATACCAAGAGGGTCACAATGATCATTTTACCTTTCCACAAACGATGGAACGCTGATCATGGTCATAGCCACCACCACCAAGACGGAGGAGGAGATGGAAACGTACCGGAAAACGTTGGTCATGGTTGGCGATTGGTTAACCAAAGGGTTTTGAAGAATGCGCCGTGTTCGGTGGCGGTTCTTGTAGACCGTGGACTTGGGTCCATTGAGGCCCAAACTTTGAGCTTAGATGGGTCGAATGTGGTTGAAAGGGTTTGTGTGATTTTCTTTGGTGGGCCTGATGACCGTGAGTCTATAGAGCTCGGCGGGAGAATGGCTGAGCATCCGGCCGTTAAAGTTACCGTTATTAGGTTTTTGGTAAGAGAAACGTTGAGGAGTACCGCCGTCACTTTACGACCGGCACCGTCTAAAGGCAAGGAGAAGAACTATGCCTTTTTAACAACCAACGTGGATCCAGAAAAAGAAAAGGAATTAGACGAAGGGGCATTGGAAGACTTCAAGAGCAAATGGAAAGAAATGGTGGAGTACAAAGAAAAGGAACCAAACAACATCATTGAAGAAATACTGTCAATAGGACAGAGTAAAGACTTTGATCTAATAGTGGTTGGAAGAGGGAGGATACCGTCGGCCGAGGTGGCGGCATTAGCTGAGCGTCAAGCTGAACATCCTGAGTTAGGTCCTATCGGAGACGTGCTCGCCTCTTCGATCAACCACATCATTCCATCAATCCTTGTGGTTCAACAACACAACAAAGCTCATGTAGAGGATATTACGGTTTCCAAAATTGTTAGTGAGTCTTCTCTAAGTATTAACGGAGACACAAATGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

842

Amino Acids

91.55

Weight (kDa)

8.93

Isoelectric Point (pI)

38.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	41 - 430	3.7e-63	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	487 - 634	6.4e-35	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	651 - 685	1.8e-06	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	795
AccB1I	GGYRCC	2 cut(s)	1316, 2098
AccI	GTMKAC	1 cut(s)	1884
AclI	AACGTT	3 cut(s)	1268, 1812, 2066
AclWI	GGATC	6 cut(s)	331, 418, 736, 784, 2144, 2157
AcoI	YGGCCR	3 cut(s)	1293, 2025, 2325
AcvI	CACGTG	1 cut(s)	1313
AcyI	GRCGYC	3 cut(s)	405, 792, 1280
AfaI	GTAC	8 cut(s)	227, 519, 798, 1191, 1218, 1803, 2077, 2225
AfiI	CCNNNNNNNGG	5 cut(s)	917, 1313, 2046, 2108, 2372
AflIII	ACRYGT	1 cut(s)	810
AgsI	TTSAA	9 cut(s)	10, 142, 944, 1006, 1852, 1946, 2197, 2255, 2438
AhdI	GACNNNNNGTC	1 cut(s)	1987
AhlI	ACTAGT	1 cut(s)	1243
AjiI	CACGTC	2 cut(s)	1015, 2389
AjnI	CCWGG	1 cut(s)	457
Alw21I	GWGCWC	4 cut(s)	543, 1185, 2001, 2394
Alw26I	GTCTC	5 cut(s)	1223, 1414, 1660, 2379, 2508
AlwI	GGATC	6 cut(s)	331, 418, 736, 784, 2144, 2157
AlwNI	CAGNNNCTG	1 cut(s)	377
AoxI	GGCC	8 cut(s)	1293, 1396, 1624, 1640, 1908, 1973, 2025, 2325
ApeKI	GCWGC	2 cut(s)	581, 584
ArsI	GACNNNNNNTTYG	2 cut(s)	1217, 1249
Asp700I	GAANNNNTTC	1 cut(s)	1371
AspLEI	GCGC	4 cut(s)	359, 526, 713, 1861
AspS9I	GGNCC	8 cut(s)	197, 232, 1397, 1641, 1899, 1909, 1973, 2375
AsuC2I	CCSGG	3 cut(s)	750, 1081, 1628
AsuHPI	GGTGA	5 cut(s)	665, 1123, 1550, 1586, 1639
AsuII	TTCGAA	1 cut(s)	1605
AvaII	GGWCC	4 cut(s)	197, 232, 1899, 2375
BalI	TGGCCA	1 cut(s)	1295
BamHI	GGATCC	1 cut(s)	2149
BanI	GGYRCC	2 cut(s)	1316, 2098
BanII	GRGCYC	4 cut(s)	543, 1185, 1534, 2001
BbrPI	CACGTG	1 cut(s)	1313
BbsI	GAAGAC	5 cut(s)	1013, 1497, 1686, 2196, 2487
Bbv12I	GWGCWC	4 cut(s)	543, 1185, 2001, 2394
BbvI	GCAGC	2 cut(s)	568, 596
BceAI	ACGGC	9 cut(s)	98, 114, 116, 1086, 1411, 1475, 1846, 2012, 2066
BcgI	CGANNNNNNTGC	2 cut(s)	1217, 1251
BciT130I	CCWGG	1 cut(s)	459
BciVI	GTATCC	1 cut(s)	2310
BclI	TGATCA	2 cut(s)	1719, 1753
BcnI	CCSGG	3 cut(s)	750, 1081, 1628
BcoDI	GTCTC	5 cut(s)	1223, 1414, 1660, 2379, 2508
BcuI	ACTAGT	1 cut(s)	1243
BfaI	CTAG	4 cut(s)	317, 1244, 1382, 1622
BfmI	CTRYAG	2 cut(s)	582, 1992
BfoI	RGCGCY	1 cut(s)	714
BfuI	GTATCC	1 cut(s)	2310
BglI	GCCNNNNNGGC	2 cut(s)	1395, 2333
BisI	GCNGC	7 cut(s)	349, 447, 582, 585, 588, 606, 2337
BlpI	GCTNAGC	2 cut(s)	2016, 2346
BlsI	GCNGC	7 cut(s)	350, 448, 583, 586, 589, 607, 2338
Bme1390I	CCNGG	4 cut(s)	459, 750, 1081, 1628
Bme18I	GGWCC	4 cut(s)	197, 232, 1899, 2375
BmeRI	GACNNNNNGTC	1 cut(s)	1987
BmgBI	CACGTC	2 cut(s)	1015, 2389
BmgT120I	GGNCC	8 cut(s)	197, 232, 1397, 1641, 1899, 1909, 1973, 2375
BmiI	GGNNCC	5 cut(s)	1318, 1900, 2100, 2151, 2238
BmrFI	CCNGG	4 cut(s)	459, 750, 1081, 1628
BmsI	GCATC	4 cut(s)	1386, 1467, 1489, 2029
BoxI	GACNNNNGTC	1 cut(s)	1897
BpiI	GAAGAC	5 cut(s)	1013, 1497, 1686, 2196, 2487
BplI	GAGNNNNNCTC	4 cut(s)	480, 512, 2376, 2408
Bpu1102I	GCTNAGC	2 cut(s)	2016, 2346
Bpu14I	TTCGAA	1 cut(s)	1605
BpuEI	CTTGAG	1 cut(s)	527
BpuMI	CCSGG	3 cut(s)	750, 1081, 1628
Bsa29I	ATCGAT	1 cut(s)	335
BsaAI	YACGTR	2 cut(s)	811, 1313
BsaHI	GRCGYC	3 cut(s)	405, 792, 1280
BsaJI	CCNNGG	5 cut(s)	447, 1362, 1393, 1888, 2328
BsaWI	WCCGGW	4 cut(s)	514, 842, 965, 1804
BsaXI	ACNNNNNCTCC	3 cut(s)	32, 876, 906
Bsc4I	CCNNNNNNNGG	5 cut(s)	917, 1313, 2046, 2108, 2372
Bse118I	RCCGGY	4 cut(s)	401, 827, 1306, 2095
BseBI	CCWGG	1 cut(s)	459
BseCI	ATCGAT	1 cut(s)	335
BseDI	CCNNGG	5 cut(s)	447, 1362, 1393, 1888, 2328
BseGI	GGATG	5 cut(s)	1053, 1377, 1437, 2020, 2362
BseLI	CCNNNNNNNGG	5 cut(s)	917, 1313, 2046, 2108, 2372
BseMII	CTCAG	5 cut(s)	572, 1026, 2007, 2337, 2358
BseRI	GAGGAG	5 cut(s)	1044, 1402, 1800, 1803, 2086
BseX3I	CGGCCG	2 cut(s)	2025, 2325
BseXI	GCAGC	2 cut(s)	568, 596
BseYI	CCCAGC	2 cut(s)	713, 1399
Bsh1285I	CGRYCG	3 cut(s)	2028, 2096, 2328
BshFI	GGCC	8 cut(s)	1295, 1398, 1626, 1642, 1910, 1975, 2027, 2327
BshNI	GGYRCC	2 cut(s)	1316, 2098
BshVI	ATCGAT	1 cut(s)	335
BsiEI	CGRYCG	3 cut(s)	2028, 2096, 2328
BsiHKAI	GWGCWC	4 cut(s)	543, 1185, 2001, 2394
BsiWI	CGTACG	1 cut(s)	796
BslI	CCNNNNNNNGG	5 cut(s)	917, 1313, 2046, 2108, 2372
BsmAI	GTCTC	5 cut(s)	1223, 1414, 1660, 2379, 2508
BsmBI	CGTCTC	2 cut(s)	1223, 2379
BsmI	GAATGC	1 cut(s)	1861
BsnI	GGCC	8 cut(s)	1295, 1398, 1626, 1642, 1910, 1975, 2027, 2327
Bsp119I	TTCGAA	1 cut(s)	1605
Bsp1286I	GDGCHC	5 cut(s)	543, 1185, 1534, 2001, 2394
Bsp1720I	GCTNAGC	2 cut(s)	2016, 2346
Bsp68I	TCGCGA	3 cut(s)	278, 767, 851
BspANI	GGCC	8 cut(s)	1295, 1398, 1626, 1642, 1910, 1975, 2027, 2327
BspCNI	CTCAG	5 cut(s)	571, 1027, 2008, 2338, 2359
BspDI	ATCGAT	1 cut(s)	335
BspLI	GGNNCC	5 cut(s)	1318, 1900, 2100, 2151, 2238
BspMAI	CTGCAG	1 cut(s)	586
BspPI	GGATC	6 cut(s)	331, 418, 736, 784, 2144, 2157
BspQI	GCTCTTC	1 cut(s)	1362
BspT104I	TTCGAA	1 cut(s)	1605
BspT107I	GGYRCC	2 cut(s)	1316, 2098
BsrFI	RCCGGY	4 cut(s)	401, 827, 1306, 2095
BssAI	RCCGGY	4 cut(s)	401, 827, 1306, 2095
BssECI	CCNNGG	5 cut(s)	447, 1362, 1393, 1888, 2328
BssNI	GRCGYC	3 cut(s)	405, 792, 1280
BssT1I	CCWWGG	1 cut(s)	1362
Bst2UI	CCWGG	1 cut(s)	459
Bst6I	CTCTTC	6 cut(s)	299, 308, 1265, 1362, 2302, 2404
BstACI	GRCGYC	3 cut(s)	405, 792, 1280
BstBAI	YACGTR	2 cut(s)	811, 1313
BstBI	TTCGAA	1 cut(s)	1605
BstC8I	GCNNGC	6 cut(s)	280, 403, 1308, 1348, 1387, 2394
BstDEI	CTNAG	8 cut(s)	558, 1020, 1035, 1924, 2016, 2346, 2367, 2501
BstDSI	CCRYGG	3 cut(s)	447, 1393, 1888
BstENI	CCTNNNNNAGG	1 cut(s)	2370
BstF5I	GGATG	5 cut(s)	1053, 1377, 1437, 2020, 2362
BstH2I	RGCGCY	1 cut(s)	714
BstHHI	GCGC	4 cut(s)	359, 526, 713, 1861
BstMAI	GTCTC	5 cut(s)	1223, 1414, 1660, 2379, 2508
BstMCI	CGRYCG	3 cut(s)	2028, 2096, 2328
BstMWI	GCNNNNNNNGC	8 cut(s)	354, 581, 587, 1136, 1323, 1395, 2333, 2342
BstNI	CCWGG	1 cut(s)	459
BstPAI	GACNNNNGTC	1 cut(s)	1897
BstSCI	CCNGG	4 cut(s)	457, 748, 1079, 1626
BstSFI	CTRYAG	2 cut(s)	582, 1992
BstV1I	GCAGC	2 cut(s)	568, 596
BstV2I	GAAGAC	5 cut(s)	1013, 1497, 1686, 2196, 2487
BstX2I	RGATCY	2 cut(s)	776, 2149
BstYI	RGATCY	2 cut(s)	776, 2149
BstZI	CGGCCG	2 cut(s)	2025, 2325
Bsu15I	ATCGAT	1 cut(s)	335
BsuI	GTATCC	1 cut(s)	2310
BsuRI	GGCC	8 cut(s)	1295, 1398, 1626, 1642, 1910, 1975, 2027, 2327
BsuTUI	ATCGAT	1 cut(s)	335
BtgI	CCRYGG	3 cut(s)	447, 1393, 1888
BtgZI	GCGATG	3 cut(s)	94, 151, 590
BtrI	CACGTC	2 cut(s)	1015, 2389
BtsCI	GGATG	5 cut(s)	1053, 1377, 1437, 2020, 2362
BtuMI	TCGCGA	3 cut(s)	278, 767, 851
Cac8I	GCNNGC	6 cut(s)	280, 403, 1308, 1348, 1387, 2394
CaiI	CAGNNNCTG	1 cut(s)	377
CfoI	GCGC	4 cut(s)	359, 526, 713, 1861
Cfr10I	RCCGGY	4 cut(s)	401, 827, 1306, 2095
Cfr13I	GGNCC	8 cut(s)	197, 232, 1397, 1641, 1899, 1909, 1973, 2375
Cfr42I	CCGCGG	1 cut(s)	450
ClaI	ATCGAT	1 cut(s)	335
CseI	GACGC	4 cut(s)	394, 1288, 1568, 2339
CsiI	ACCWGGT	1 cut(s)	457
Csp6I	GTAC	8 cut(s)	226, 518, 797, 1190, 1217, 1802, 2076, 2224
CspCI	CAANNNNNGTGG	4 cut(s)	2127, 2162, 2412, 2447
CviAII	CATG	8 cut(s)	220, 229, 484, 1571, 1693, 1757, 1820, 2456
CviQI	GTAC	8 cut(s)	226, 518, 797, 1190, 1217, 1802, 2076, 2224
DdeI	CTNAG	8 cut(s)	558, 1020, 1035, 1924, 2016, 2346, 2367, 2501
DraI	TTTAAA	1 cut(s)	66
DriI	GACNNNNNGTC	1 cut(s)	1987
EaeI	YGGCCR	3 cut(s)	1293, 2025, 2325
EagI	CGGCCG	2 cut(s)	2025, 2325
Eam1104I	CTCTTC	6 cut(s)	299, 308, 1265, 1362, 2302, 2404
Eam1105I	GACNNNNNGTC	1 cut(s)	1987
EarI	CTCTTC	6 cut(s)	299, 308, 1265, 1362, 2302, 2404
Ecl136II	GAGCTC	3 cut(s)	541, 1183, 1999
EclXI	CGGCCG	2 cut(s)	2025, 2325
Eco130I	CCWWGG	1 cut(s)	1362
Eco24I	GRGCYC	4 cut(s)	543, 1185, 1534, 2001
Eco47I	GGWCC	4 cut(s)	197, 232, 1899, 2375
Eco52I	CGGCCG	2 cut(s)	2025, 2325
Eco53kI	GAGCTC	3 cut(s)	541, 1183, 1999
Eco72I	CACGTG	1 cut(s)	1313
EcoICRI	GAGCTC	3 cut(s)	541, 1183, 1999
EcoNI	CCTNNNNNAGG	1 cut(s)	2370
EcoO109I	RGGNCCY	1 cut(s)	2375
EcoRII	CCWGG	1 cut(s)	457
EcoT14I	CCWWGG	1 cut(s)	1362
EcoT22I	ATGCAT	1 cut(s)	1482
EcoT38I	GRGCYC	4 cut(s)	543, 1185, 1534, 2001
ErhI	CCWWGG	1 cut(s)	1362
Esp3I	CGTCTC	2 cut(s)	1223, 2379
FaeI	CATG	8 cut(s)	223, 232, 487, 1574, 1696, 1760, 1823, 2459
FatI	CATG	8 cut(s)	219, 228, 483, 1570, 1692, 1756, 1819, 2455
FauI	CCCGC	2 cut(s)	634, 1997
FbaI	TGATCA	2 cut(s)	1719, 1753
FblI	GTMKAC	1 cut(s)	1884
Fnu4HI	GCNGC	7 cut(s)	349, 447, 582, 585, 588, 606, 2337
FokI	GGATG	5 cut(s)	1060, 1364, 1424, 2007, 2349
FriOI	GRGCYC	4 cut(s)	543, 1185, 1534, 2001
Fsp4HI	GCNGC	7 cut(s)	349, 447, 582, 585, 588, 606, 2337
FspBI	CTAG	4 cut(s)	317, 1244, 1382, 1622
GlaI	GCGC	4 cut(s)	358, 525, 712, 1860
GluI	GCNGC	7 cut(s)	349, 447, 582, 585, 588, 606, 2337
GsaI	CCCAGC	2 cut(s)	717, 1403
HaeII	RGCGCY	1 cut(s)	714
HaeIII	GGCC	8 cut(s)	1295, 1398, 1626, 1642, 1910, 1975, 2027, 2327
HgaI	GACGC	4 cut(s)	394, 1288, 1568, 2339
HhaI	GCGC	4 cut(s)	359, 526, 713, 1861
Hin1I	GRCGYC	3 cut(s)	405, 792, 1280
Hin1II	CATG	8 cut(s)	223, 232, 487, 1574, 1696, 1760, 1823, 2459
Hin6I	GCGC	4 cut(s)	357, 524, 711, 1859
HinP1I	GCGC	4 cut(s)	357, 524, 711, 1859
HincII	GTYRAC	4 cut(s)	155, 1143, 1619, 1837
HindII	GTYRAC	4 cut(s)	155, 1143, 1619, 1837
HindIII	AAGCTT	1 cut(s)	545
HpaI	GTTAAC	1 cut(s)	1837
HphI	GGTGA	5 cut(s)	665, 1123, 1550, 1586, 1639
Hpy166II	GTNNAC	8 cut(s)	155, 971, 1143, 1558, 1619, 1837, 1885, 1892
Hpy8I	GTNNAC	8 cut(s)	155, 971, 1143, 1558, 1619, 1837, 1885, 1892
HpyAV	CCTTC	2 cut(s)	16, 2171
HpyCH4V	TGCA	5 cut(s)	584, 1238, 1480, 1525, 1675
HpyF10VI	GCNNNNNNNGC	8 cut(s)	354, 581, 587, 1136, 1323, 1395, 2333, 2342
HpyF3I	CTNAG	8 cut(s)	558, 1020, 1035, 1924, 2016, 2346, 2367, 2501
Hsp92I	GRCGYC	3 cut(s)	405, 792, 1280
Hsp92II	CATG	8 cut(s)	223, 232, 487, 1574, 1696, 1760, 1823, 2459
HspAI	GCGC	4 cut(s)	357, 524, 711, 1859
KroI	GCCGGC	2 cut(s)	401, 1306
KroNI	GCCGGC	2 cut(s)	403, 1308
Ksp22I	TGATCA	2 cut(s)	1719, 1753
KspAI	GTTAAC	1 cut(s)	1837
KspI	CCGCGG	1 cut(s)	450
LguI	GCTCTTC	1 cut(s)	1362
LmnI	GCTCC	3 cut(s)	884, 1031, 1180
Lsp1109I	GCAGC	2 cut(s)	568, 596
LweI	GCATC	4 cut(s)	1386, 1467, 1489, 2029
MabI	ACCWGGT	1 cut(s)	457
MaeI	CTAG	4 cut(s)	317, 1244, 1382, 1622
MaeIII	GTNAC	9 cut(s)	81, 428, 461, 1066, 1562, 1580, 1711, 2035, 2083
MflI	RGATCY	2 cut(s)	776, 2149
MhlI	GDGCHC	5 cut(s)	543, 1185, 1534, 2001, 2394
MlsI	TGGCCA	1 cut(s)	1295
MluCI	AATT	5 cut(s)	381, 861, 948, 2168, 2481
MluNI	TGGCCA	1 cut(s)	1295
MmeI	TCCRAC	6 cut(s)	160, 364, 388, 769, 1072, 2284
Mox20I	TGGCCA	1 cut(s)	1295
Mph1103I	ATGCAT	1 cut(s)	1482
MreI	CGCCGGCG	1 cut(s)	401
MroNI	GCCGGC	2 cut(s)	401, 1306
MroXI	GAANNNNTTC	1 cut(s)	1371
MscI	TGGCCA	1 cut(s)	1295
MslI	CAYNNNNRTG	1 cut(s)	1520
Msp20I	TGGCCA	1 cut(s)	1295
MspA1I	CMGCKG	1 cut(s)	449
MspR9I	CCNGG	4 cut(s)	459, 750, 1081, 1628
Mva1269I	GAATGC	1 cut(s)	1861
MvaI	CCWGG	1 cut(s)	459
MwoI	GCNNNNNNNGC	8 cut(s)	354, 581, 587, 1136, 1323, 1395, 2333, 2342
NaeI	GCCGGC	2 cut(s)	403, 1308
NciI	CCSGG	3 cut(s)	750, 1081, 1628
NgoMIV	GCCGGC	2 cut(s)	401, 1306
NlaIII	CATG	8 cut(s)	223, 232, 487, 1574, 1696, 1760, 1823, 2459
NlaIV	GGNNCC	5 cut(s)	1318, 1900, 2100, 2151, 2238
NmeAIII	GCCGAG	4 cut(s)	196, 493, 1980, 2353
NmuCI	GTSAC	3 cut(s)	1580, 1711, 2083
NruI	TCGCGA	3 cut(s)	278, 767, 851
NsiI	ATGCAT	1 cut(s)	1482
NspV	TTCGAA	1 cut(s)	1605
PciSI	GCTCTTC	1 cut(s)	1362
PcsI	WCGNNNNNNNCGW	2 cut(s)	798, 1274
PctI	GAATGC	1 cut(s)	1861
PdiI	GCCGGC	2 cut(s)	403, 1308
PdmI	GAANNNNTTC	1 cut(s)	1371
PfeI	GAWTC	3 cut(s)	269, 366, 868
Pfl23II	CGTACG	1 cut(s)	796
PkrI	GCNGC	7 cut(s)	350, 448, 583, 586, 589, 607, 2338
PmaCI	CACGTG	1 cut(s)	1313
PmlI	CACGTG	1 cut(s)	1313
Ppu21I	YACGTR	2 cut(s)	811, 1313
PpuMI	RGGWCCY	1 cut(s)	2375
PshAI	GACNNNNGTC	1 cut(s)	1897
Psp124BI	GAGCTC	3 cut(s)	543, 1185, 2001
Psp1406I	AACGTT	3 cut(s)	1268, 1812, 2066
Psp5II	RGGWCCY	1 cut(s)	2375
Psp6I	CCWGG	1 cut(s)	457
PspCI	CACGTG	1 cut(s)	1313
PspFI	CCCAGC	2 cut(s)	713, 1399
PspGI	CCWGG	1 cut(s)	457
PspLI	CGTACG	1 cut(s)	796
PspN4I	GGNNCC	5 cut(s)	1318, 1900, 2100, 2151, 2238
PspPI	GGNCC	8 cut(s)	197, 232, 1397, 1641, 1899, 1909, 1973, 2375
PspPPI	RGGWCCY	1 cut(s)	2375
PstI	CTGCAG	1 cut(s)	586
PstNI	CAGNNNCTG	1 cut(s)	377
PsuI	RGATCY	2 cut(s)	776, 2149
RruI	TCGCGA	3 cut(s)	278, 767, 851
RsaI	GTAC	8 cut(s)	227, 519, 798, 1191, 1218, 1803, 2077, 2225
RsaNI	GTAC	8 cut(s)	226, 518, 797, 1190, 1217, 1802, 2076, 2224
RseI	CAYNNNNRTG	1 cut(s)	1520
SacI	GAGCTC	3 cut(s)	543, 1185, 2001
SacII	CCGCGG	1 cut(s)	450
SapI	GCTCTTC	1 cut(s)	1362
SatI	GCNGC	7 cut(s)	349, 447, 582, 585, 588, 606, 2337
Sau96I	GGNCC	8 cut(s)	197, 232, 1397, 1641, 1899, 1909, 1973, 2375
ScrFI	CCNGG	4 cut(s)	459, 750, 1081, 1628
SduI	GDGCHC	5 cut(s)	543, 1185, 1534, 2001, 2394
SexAI	ACCWGGT	1 cut(s)	457
SfaNI	GCATC	4 cut(s)	1386, 1467, 1489, 2029
SfcI	CTRYAG	2 cut(s)	582, 1992
Sfr303I	CCGCGG	1 cut(s)	450
SfuI	TTCGAA	1 cut(s)	1605
SgrAI	CRCCGGYG	1 cut(s)	401
SgrBI	CCGCGG	1 cut(s)	450
SinI	GGWCC	4 cut(s)	197, 232, 1899, 2375
SmiMI	CAYNNNNRTG	1 cut(s)	1520
SmlI	CTYRAG	1 cut(s)	542
SmoI	CTYRAG	1 cut(s)	542
SpeI	ACTAGT	1 cut(s)	1243
Sse9I	AATT	5 cut(s)	381, 861, 948, 2168, 2481
SspMI	CTAG	4 cut(s)	317, 1244, 1382, 1622
SstI	GAGCTC	3 cut(s)	543, 1185, 2001
StyD4I	CCNGG	4 cut(s)	457, 748, 1079, 1626
StyI	CCWWGG	1 cut(s)	1362
TaqI	TCGA	9 cut(s)	267, 324, 335, 500, 875, 1431, 1605, 1934, 2402
TaqII	GACCGA	1 cut(s)	302
TasI	AATT	5 cut(s)	381, 861, 948, 2168, 2481
TatI	WGTACW	2 cut(s)	1189, 2223
TauI	GCSGC	5 cut(s)	351, 449, 590, 608, 2339
TfiI	GAWTC	3 cut(s)	269, 366, 868
TseFI	GTSAC	3 cut(s)	1580, 1711, 2083
TseI	GCWGC	2 cut(s)	581, 584
Tsp45I	GTSAC	3 cut(s)	1580, 1711, 2083
TspDTI	ATGAA	5 cut(s)	472, 770, 860, 1175, 1261
VpaK11BI	GGWCC	4 cut(s)	197, 232, 1899, 2375
XagI	CCTNNNNNAGG	1 cut(s)	2370
XbaI	TCTAGA	1 cut(s)	316
XmiI	GTMKAC	1 cut(s)	1884
XmnI	GAANNNNTTC	1 cut(s)	1371
XspI	CTAG	4 cut(s)	317, 1244, 1382, 1622
ZraI	GACGTC	1 cut(s)	793
Zsp2I	ATGCAT	1 cut(s)	1482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.