Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Reverse (-)

21087236 .. 21090933

Gene Structure 3698 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1124400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGCACTCAATTTTACCAACATGAGAACTTCATCTGATGGAGCTTGGCAAGGTGATAATCCTCTTCACCATGCCTTTCCATTATTAATAGTTCAAACCCTTCTCATCCTCTTCGTTAGCCGCTTCCTCGCCTTCCTTCTCAAGCCACTCCGCCAACCTAAAGTCATTGCCGAGATATTAGGTGGGATTTTGTTGGGACCATCAGCACTTGGCAGGAATAAAGATTTCTTGGAGATCTTATTCCCTTCATGGAGCACTCCCATACTCGAATCCGTTGCAAGCATTGGCCTTCTCTTTTACCTATTTCTCGTCGGCCTCGAGCTTGATCTGATCTCGATTCGCCGCAGTGGCAGAAGAGCCATCAGCATAGCACTCGCAGGCATTTCCCTCCCTTTCTTCTTCGGAGTTGGTCTCGCATTCTTTACCCGAAAAGTCATTCACGGAGAGAACAAGGTCGGTTACCCTCAGCTTCTCGTGTTCACAGGGGTAGCCCTCTCCATCACCGCTTTCCCTGTCCTCGCGCGCATCTTAGCGGAGCTCAAACTTCTCACAACTCGTGTCGGTGAAACTGCGATGGCAGCAGCAGCCTTCAACGATATAGCTGCATGGATCCTGCTGGCATTGGCGGTGGCCTTAGCCGGCGGGAGCAACAAAAGCCCCTTAGTGTCCATTTGGGTTCTCATTTCCGGCGTTGGTTTTGTTGCTTTTCAGCTAACTTTGGTCCGACCTTTCATGAACTGGGTTGCCAGACGGTGCTCATCCGATCATGACCTAGTGGATGAGGCTTACATCTGCTTAACCCTAGCCGGGGTACTGTTATCCGGGTTTATGACAGACATGATCGGCATCCATGCCATATTCGGCGCTTTTGTTTTCGGGTTAACGATTCCAAAGGGGGGAGAGTTTGCAGCCAGACTGACCAAAAGGATAGAGGATTTTGTGTCTGGCTTGCTTCTTCCTCTATATTTTGCTTCAAGTGGTTTAAAAACTGATGTTGCTAAAATTCAAGGGCTCAGGGCATGGGGGTTGGTGGCTTTGGTCATATCTGTGTCCTGCACTGGCAAGATTTTGGGTACTTTTCTGGTGGCCATGTTGTTTATGATACCAGTAAGAGAGTCATTAGCACTGGGAGTCCTCATGAATACCAAAGGCTTGGTGGAGCTCATTGTTCTCAACATTGGGAAGGAAAAAAAGATTCTAAACGATGAGATGTTTGCTATTTTGACCCTGATGGCAGTTTTTACCACCTTCATGACAACTCCAGCAGTGATGGCCATTTACAAACCCTCATGGGATGTCATTGCTTCTCAAACTCCCCGTCTCTTACAGGGACAACATTCTCCGACCAAACCCGCCCACAGACGAAAGGACCTTCGAATCCTTGCTTGCGTTCATGGACCTGGAAGCGTACCCTCCCTCGTCAACCTCATCGAGTCAGTCGCACCTGCCTCACAAGACTCATCGCCGCTCAAAGTTTACGTGATGCATCTTGTTGAGCTCACCGACCGTTCATCGTCCATTATGATGGTACATCGAGCTCGGAAAAATGGTTTTCCCTTCATCAACCGCTTATGTCGAGGGGTCTCACAGGACCAAATCCCTGCGGCGTTTGAGGCGTATGGTCAAGTCGGTCAAGTCACCACCCGCCACACAACGGCCATCTCCGCGTTGTCCACAATGCAAGAGGATATTCTCCATGTTGCAGAGGCGAAGACGGTTTCCCTCGTCATCTTGCCTTTTAACAAACAATGGAGAAGAGAGGAAGAGAAGGAAATGGAGGTTCTGGGGGATGACTGGAAAAGGGTCAATCAGAGGGTCTTAAGTACTGCAAATTGCTCAGTGGCATTGTTCGTGGATCGTGGATTTGGCAGCAGACGTGAGAGGGGTGCCGCCACTCCCAAGAGGGTGTGCATTTTGTTCTTGGGGGGACCTGATGACCGGGAGGCTCTGGAATTTGGTGGAAGATTGGCACATTCAACCGTTATGGTAACAGTTGTAAAATGCATTACGGACAGCTTGGAGCAAAATCATGAGAAGGAACTAGATGAGGCAGCAGTTGCAGAGTTGAGGAGGTGTAATGAATCAGTGGAATACATAGAGAAGGTAGTGACAGGCGGCATCACGGGGGCGGCGGTGAGTATAGGGCTGAGTAGGGAATACGACCTGGTTATCGTGGGGAAGGAGCAGCAGAATTCTGAATTGGCTCGCCATCGGCTGGAGCATGCTGAACTGGGACAAATTGGAGACACATTAGCATCGTCACCTGGGATTTCGTCCTCTGTTCTTGTGATACAAACACCAAGAACCCGCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

772

Amino Acids

84.1

Weight (kDa)

8.99

Isoelectric Point (pI)

40.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	37 - 422	3.1e-61	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	490 - 624	3.3e-31	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	636 - 765	1.5e-07	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1453
Acc36I	ACCTGC	1 cut(s)	1453
AccB1I	GGYRCC	1 cut(s)	1886
AccB7I	CCANNNNNTGG	1 cut(s)	2215
AccBSI	CCGCTC	1 cut(s)	1468
AccII	CGCG	3 cut(s)	521, 523, 1667
AclWI	GGATC	3 cut(s)	604, 617, 1863
AcoI	YGGCCR	3 cut(s)	1086, 1272, 1656
AcsI	RAATTY	3 cut(s)	1002, 1952, 2191
AfaI	GTAC	5 cut(s)	813, 1075, 1410, 1530, 1825
AfiI	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
AflII	CTTAAG	1 cut(s)	1819
AgsI	TTSAA	5 cut(s)	95, 592, 975, 1007, 1977
AjiI	CACGTC	1 cut(s)	1877
AjnI	CCWGG	3 cut(s)	1399, 2163, 2263
AjuI	GAANNNNNNNTTGG	2 cut(s)	2183, 2215
Alw21I	GWGCWC	6 cut(s)	257, 540, 758, 1164, 1500, 1540
Alw26I	GTCTC	4 cut(s)	416, 1325, 1588, 2238
AlwI	GGATC	3 cut(s)	604, 617, 1863
Ama87I	CYCGRG	1 cut(s)	317
AoxI	GGCC	6 cut(s)	286, 313, 630, 1086, 1272, 1656
ApeKI	GCWGC	8 cut(s)	578, 581, 584, 602, 908, 1869, 2051, 2185
ApoI	RAATTY	3 cut(s)	1002, 1952, 2191
ArsI	GACNNNNNNTTYG	2 cut(s)	1303, 1335
AseI	ATTAAT	1 cut(s)	86
AspLEI	GCGC	3 cut(s)	523, 525, 866
AspS9I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
AsuC2I	CCSGG	3 cut(s)	808, 823, 1940
AsuHPI	GGTGA	8 cut(s)	59, 65, 493, 575, 1492, 1630, 2146, 2253
AsuII	TTCGAA	1 cut(s)	1375
AvaI	CYCGRG	1 cut(s)	317
AvaII	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
BalI	TGGCCA	2 cut(s)	1088, 1274
BamHI	GGATCC	1 cut(s)	609
BanI	GGYRCC	1 cut(s)	1886
BanII	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
BauI	CACGAG	2 cut(s)	473, 555
BbsI	GAAGAC	1 cut(s)	1718
Bbv12I	GWGCWC	6 cut(s)	257, 540, 758, 1164, 1500, 1540
BbvCI	CCTCAGC	1 cut(s)	465
BbvI	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
BceAI	ACGGC	1 cut(s)	1671
BcgI	CGANNNNNNTGC	6 cut(s)	257, 291, 355, 389, 584, 618
BciT130I	CCWGG	3 cut(s)	1401, 2165, 2265
BcnI	CCSGG	3 cut(s)	808, 823, 1940
BcoDI	GTCTC	4 cut(s)	416, 1325, 1588, 2238
BfaI	CTAG	3 cut(s)	773, 803, 2042
BfoI	RGCGCY	1 cut(s)	867
BfrI	CTTAAG	1 cut(s)	1819
BfuAI	ACCTGC	1 cut(s)	1453
BglI	GCCNNNNNGGC	1 cut(s)	348
BglII	AGATCT	1 cut(s)	234
BmcAI	AGTACT	1 cut(s)	1825
Bme1390I	CCNGG	6 cut(s)	808, 823, 1401, 1940, 2165, 2265
Bme18I	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
BmeT110I	CYCGRG	1 cut(s)	317
BmgBI	CACGTC	1 cut(s)	1877
BmgT120I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
BmiI	GGNNCC	4 cut(s)	198, 611, 1888, 1929
BmrFI	CCNGG	6 cut(s)	808, 823, 1401, 1940, 2165, 2265
BmrI	ACTGGG	3 cut(s)	748, 1136, 2240
BmsI	GCATC	6 cut(s)	534, 855, 1473, 1495, 2127, 2264
BmuI	ACTGGG	3 cut(s)	748, 1136, 2240
BpiI	GAAGAC	1 cut(s)	1718
BplI	GAGNNNNNCTC	2 cut(s)	396, 428
BpmI	CTGGAG	2 cut(s)	1245, 2237
Bpu10I	CCTNAGC	3 cut(s)	465, 634, 1013
Bpu14I	TTCGAA	1 cut(s)	1375
BpuEI	CTTGAG	1 cut(s)	125
BpuMI	CCSGG	3 cut(s)	808, 823, 1940
BsaAI	YACGTR	1 cut(s)	1480
BsaBI	GATNNNNATC	1 cut(s)	845
BsaI	GGTCTC	2 cut(s)	416, 1588
BsaJI	CCNNGG	2 cut(s)	807, 2264
Bsc4I	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
Bse118I	RCCGGY	1 cut(s)	638
Bse1I	ACTGG	6 cut(s)	743, 1063, 1106, 1131, 1799, 2235
Bse3DI	GCAATG	2 cut(s)	165, 1299
Bse8I	GATNNNNATC	1 cut(s)	845
BseBI	CCWGG	3 cut(s)	1401, 2165, 2265
BseDI	CCNNGG	2 cut(s)	807, 2264
BseGI	GGATG	6 cut(s)	105, 758, 784, 846, 1300, 1795
BseJI	GATNNNNATC	1 cut(s)	845
BseLI	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
BseMI	GCAATG	2 cut(s)	165, 1299
BseMII	CTCAG	4 cut(s)	479, 1027, 1851, 2138
BseNI	ACTGG	6 cut(s)	743, 1063, 1106, 1131, 1799, 2235
BsePI	GCGCGC	1 cut(s)	521
BseRI	GAGGAG	1 cut(s)	2083
BseXI	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
BsgI	GTGCAG	1 cut(s)	1039
Bsh1236I	CGCG	3 cut(s)	521, 523, 1667
Bsh1285I	CGRYCG	1 cut(s)	1507
BshFI	GGCC	6 cut(s)	288, 315, 632, 1088, 1274, 1658
BshNI	GGYRCC	1 cut(s)	1886
BsiEI	CGRYCG	1 cut(s)	1507
BsiHKAI	GWGCWC	6 cut(s)	257, 540, 758, 1164, 1500, 1540
BsiHKCI	CYCGRG	1 cut(s)	317
BsiSI	CCGG	5 cut(s)	639, 687, 807, 822, 1939
BslFI	GGGAC	4 cut(s)	210, 1344, 1941, 2247
BslI	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
BsmAI	GTCTC	4 cut(s)	416, 1325, 1588, 2238
BsmBI	CGTCTC	1 cut(s)	1325
BsmFI	GGGAC	4 cut(s)	210, 1344, 1941, 2247
BsmI	GAATGC	1 cut(s)	416
BsnI	GGCC	6 cut(s)	288, 315, 632, 1088, 1274, 1658
Bso31I	GGTCTC	2 cut(s)	416, 1588
BsoBI	CYCGRG	1 cut(s)	317
Bsp119I	TTCGAA	1 cut(s)	1375
Bsp1286I	GDGCHC	7 cut(s)	257, 540, 758, 1014, 1164, 1500, 1540
Bsp143I	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
BspANI	GGCC	6 cut(s)	288, 315, 632, 1088, 1274, 1658
BspCNI	CTCAG	4 cut(s)	478, 1026, 1850, 2139
BspFNI	CGCG	3 cut(s)	521, 523, 1667
BspHI	TCATGA	5 cut(s)	732, 766, 1137, 1251, 2029
BspLI	GGNNCC	4 cut(s)	198, 611, 1888, 1929
BspMI	ACCTGC	1 cut(s)	1453
BspPI	GGATC	3 cut(s)	604, 617, 1863
BspQI	GCTCTTC	1 cut(s)	349
BspT104I	TTCGAA	1 cut(s)	1375
BspT107I	GGYRCC	1 cut(s)	1886
BspTI	CTTAAG	1 cut(s)	1819
BspTNI	GGTCTC	2 cut(s)	416, 1588
BsrBI	CCGCTC	1 cut(s)	1468
BsrDI	GCAATG	2 cut(s)	165, 1299
BsrFI	RCCGGY	1 cut(s)	638
BsrI	ACTGG	6 cut(s)	743, 1063, 1106, 1131, 1799, 2235
BssAI	RCCGGY	1 cut(s)	638
BssECI	CCNNGG	2 cut(s)	807, 2264
BssHII	GCGCGC	1 cut(s)	521
BssMI	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
BssSI	CACGAG	2 cut(s)	473, 555
Bst2BI	CACGAG	2 cut(s)	473, 555
Bst2UI	CCWGG	3 cut(s)	1401, 2165, 2265
Bst4CI	ACNGT	6 cut(s)	753, 816, 1508, 1717, 1981, 1993
Bst6I	CTCTTC	5 cut(s)	69, 116, 349, 1750, 1758
BstAFI	CTTAAG	1 cut(s)	1819
BstAPI	GCANNNNNTGC	1 cut(s)	2057
BstBAI	YACGTR	1 cut(s)	1480
BstBI	TTCGAA	1 cut(s)	1375
BstC8I	GCNNGC	9 cut(s)	280, 379, 523, 618, 640, 950, 1387, 2206, 2223
BstDEI	CTNAG	7 cut(s)	465, 529, 634, 661, 1013, 1837, 2147
BstEII	GGTNACC	1 cut(s)	458
BstF5I	GGATG	6 cut(s)	105, 758, 784, 846, 1300, 1795
BstFNI	CGCG	3 cut(s)	521, 523, 1667
BstH2I	RGCGCY	1 cut(s)	867
BstHHI	GCGC	3 cut(s)	523, 525, 866
BstKTI	GATC	7 cut(s)	237, 328, 333, 612, 766, 843, 1858
BstMAI	GTCTC	4 cut(s)	416, 1325, 1588, 2238
BstMBI	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
BstMCI	CGRYCG	1 cut(s)	1507
BstMWI	GCNNNNNNNGC	8 cut(s)	348, 578, 584, 654, 1271, 1613, 1664, 2057
BstNI	CCWGG	3 cut(s)	1401, 2165, 2265
BstNSI	RCATGY	1 cut(s)	2225
BstPI	GGTNACC	1 cut(s)	458
BstSCI	CCNGG	6 cut(s)	806, 821, 1399, 1938, 2163, 2263
BstUI	CGCG	3 cut(s)	521, 523, 1667
BstV1I	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
BstV2I	GAAGAC	1 cut(s)	1718
BstX2I	RGATCY	2 cut(s)	234, 609
BstXI	CCANNNNNNTGG	2 cut(s)	1153, 1525
BstYI	RGATCY	2 cut(s)	234, 609
BsuRI	GGCC	6 cut(s)	288, 315, 632, 1088, 1274, 1658
BtgZI	GCGATG	2 cut(s)	587, 1446
BtrI	CACGTC	1 cut(s)	1877
BtsCI	GGATG	6 cut(s)	105, 758, 784, 846, 1300, 1795
BtsI	GCAGTG	2 cut(s)	352, 1272
BtsIMutI	CAGTG	6 cut(s)	352, 1056, 1124, 1272, 1845, 2091
BveI	ACCTGC	1 cut(s)	1453
Cac8I	GCNNGC	9 cut(s)	280, 379, 523, 618, 640, 950, 1387, 2206, 2223
CciI	TCATGA	5 cut(s)	732, 766, 1137, 1251, 2029
CfoI	GCGC	3 cut(s)	523, 525, 866
Cfr10I	RCCGGY	1 cut(s)	638
Cfr13I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
CsiI	ACCWGGT	1 cut(s)	2163
Csp6I	GTAC	5 cut(s)	812, 1074, 1409, 1529, 1824
CviQI	GTAC	5 cut(s)	812, 1074, 1409, 1529, 1824
DdeI	CTNAG	7 cut(s)	465, 529, 634, 661, 1013, 1837, 2147
DpnI	GATC	7 cut(s)	236, 327, 332, 611, 765, 842, 1857
DpnII	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
DraI	TTTAAA	1 cut(s)	984
EaeI	YGGCCR	3 cut(s)	1086, 1272, 1656
Eam1104I	CTCTTC	5 cut(s)	69, 116, 349, 1750, 1758
EarI	CTCTTC	5 cut(s)	69, 116, 349, 1750, 1758
EciI	GGCGGA	1 cut(s)	140
Ecl136II	GAGCTC	4 cut(s)	538, 1162, 1498, 1538
Eco24I	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
Eco31I	GGTCTC	2 cut(s)	416, 1588
Eco47I	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
Eco53kI	GAGCTC	4 cut(s)	538, 1162, 1498, 1538
Eco88I	CYCGRG	1 cut(s)	317
Eco91I	GGTNACC	1 cut(s)	458
EcoICRI	GAGCTC	4 cut(s)	538, 1162, 1498, 1538
EcoO109I	RGGNCCY	2 cut(s)	1369, 1928
EcoO65I	GGTNACC	1 cut(s)	458
EcoRI	GAATTC	1 cut(s)	2191
EcoRII	CCWGG	3 cut(s)	1399, 2163, 2263
EcoT22I	ATGCAT	2 cut(s)	1488, 2006
EcoT38I	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
Esp3I	CGTCTC	1 cut(s)	1325
FaqI	GGGAC	4 cut(s)	210, 1344, 1941, 2247
FauI	CCCGC	3 cut(s)	635, 1360, 1652
FokI	GGATG	6 cut(s)	92, 745, 791, 833, 1307, 1802
FriOI	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
FspBI	CTAG	3 cut(s)	773, 803, 2042
GlaI	GCGC	3 cut(s)	522, 524, 865
GsuI	CTGGAG	2 cut(s)	1245, 2237
HaeII	RGCGCY	1 cut(s)	867
HaeIII	GGCC	6 cut(s)	288, 315, 632, 1088, 1274, 1658
HapII	CCGG	5 cut(s)	639, 687, 807, 822, 1939
HhaI	GCGC	3 cut(s)	523, 525, 866
Hin6I	GCGC	3 cut(s)	521, 523, 864
HinP1I	GCGC	3 cut(s)	521, 523, 864
HincII	GTYRAC	2 cut(s)	882, 1423
HindII	GTYRAC	2 cut(s)	882, 1423
HpaI	GTTAAC	1 cut(s)	882
HpaII	CCGG	5 cut(s)	639, 687, 807, 822, 1939
HphI	GGTGA	8 cut(s)	59, 65, 493, 575, 1492, 1630, 2146, 2253
Hpy166II	GTNNAC	5 cut(s)	480, 882, 1423, 1477, 1674
Hpy188I	TCNGA	9 cut(s)	37, 330, 404, 725, 763, 1344, 1542, 1812, 2197
Hpy188III	TCNNGA	7 cut(s)	334, 733, 767, 1138, 1252, 1949, 2030
Hpy8I	GTNNAC	5 cut(s)	480, 882, 1423, 1477, 1674
Hpy99I	CGWCG	1 cut(s)	314
HpyCH4III	ACNGT	6 cut(s)	753, 816, 1508, 1717, 1981, 1993
HpyCH4IV	ACGT	2 cut(s)	1479, 1876
HpyF10VI	GCNNNNNNNGC	8 cut(s)	348, 578, 584, 654, 1271, 1613, 1664, 2057
HpyF3I	CTNAG	7 cut(s)	465, 529, 634, 661, 1013, 1837, 2147
HpySE526I	ACGT	2 cut(s)	1479, 1876
HspAI	GCGC	3 cut(s)	521, 523, 864
KroI	GCCGGC	1 cut(s)	638
KroNI	GCCGGC	1 cut(s)	640
KspAI	GTTAAC	1 cut(s)	882
Kzo9I	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
LguI	GCTCTTC	1 cut(s)	349
LmnI	GCTCC	8 cut(s)	41, 252, 535, 645, 1159, 2020, 2182, 2218
Lsp1109I	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
LweI	GCATC	6 cut(s)	534, 855, 1473, 1495, 2127, 2264
MabI	ACCWGGT	1 cut(s)	2163
MaeI	CTAG	3 cut(s)	773, 803, 2042
MaeII	ACGT	2 cut(s)	1479, 1876
MaeIII	GTNAC	5 cut(s)	458, 1636, 1987, 2107, 2259
MalI	GATC	7 cut(s)	236, 327, 332, 611, 765, 842, 1857
MbiI	CCGCTC	1 cut(s)	1468
MboI	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
MflI	RGATCY	2 cut(s)	234, 609
MhlI	GDGCHC	7 cut(s)	257, 540, 758, 1014, 1164, 1500, 1540
MlsI	TGGCCA	2 cut(s)	1088, 1274
MluCI	AATT	7 cut(s)	10, 1002, 1831, 1952, 2191, 2198, 2238
MluNI	TGGCCA	2 cut(s)	1088, 1274
MlyI	GAGTC	4 cut(s)	1124, 1140, 1442, 1451
MmeI	TCCRAC	2 cut(s)	748, 1367
Mox20I	TGGCCA	2 cut(s)	1088, 1274
Mph1103I	ATGCAT	2 cut(s)	1488, 2006
MroNI	GCCGGC	1 cut(s)	638
MscI	TGGCCA	2 cut(s)	1088, 1274
MseI	TTAA	6 cut(s)	86, 797, 881, 983, 1740, 1820
MslI	CAYNNNNRTG	3 cut(s)	1046, 1250, 1523
Msp20I	TGGCCA	2 cut(s)	1088, 1274
MspCI	CTTAAG	1 cut(s)	1819
MspI	CCGG	5 cut(s)	639, 687, 807, 822, 1939
MspR9I	CCNGG	6 cut(s)	808, 823, 1401, 1940, 2165, 2265
Mva1269I	GAATGC	1 cut(s)	416
MvaI	CCWGG	3 cut(s)	1401, 2165, 2265
MvnI	CGCG	3 cut(s)	521, 523, 1667
MwoI	GCNNNNNNNGC	8 cut(s)	348, 578, 584, 654, 1271, 1613, 1664, 2057
NaeI	GCCGGC	1 cut(s)	640
NciI	CCSGG	3 cut(s)	808, 823, 1940
NdeII	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
NgoMIV	GCCGGC	1 cut(s)	638
NlaIV	GGNNCC	4 cut(s)	198, 611, 1888, 1929
NmeAIII	GCCGAG	1 cut(s)	196
NmuCI	GTSAC	3 cut(s)	1636, 2107, 2259
NsiI	ATGCAT	2 cut(s)	1488, 2006
NspI	RCATGY	1 cut(s)	2225
NspV	TTCGAA	1 cut(s)	1375
PaeI	GCATGC	1 cut(s)	2225
PaeR7I	CTCGAG	1 cut(s)	317
PagI	TCATGA	5 cut(s)	732, 766, 1137, 1251, 2029
PaqCI	CACCTGC	1 cut(s)	1453
PauI	GCGCGC	1 cut(s)	521
PciSI	GCTCTTC	1 cut(s)	349
PcsI	WCGNNNNNNNCGW	1 cut(s)	315
PctI	GAATGC	1 cut(s)	416
PdiI	GCCGGC	1 cut(s)	640
PfeI	GAWTC	6 cut(s)	269, 337, 886, 1195, 1377, 2081
PflMI	CCANNNNNTGG	1 cut(s)	2215
PleI	GAGTC	4 cut(s)	1123, 1139, 1441, 1451
PpsI	GAGTC	4 cut(s)	1123, 1139, 1441, 1451
Ppu21I	YACGTR	1 cut(s)	1480
PpuMI	RGGWCCY	2 cut(s)	1369, 1928
PshBI	ATTAAT	1 cut(s)	86
Psp124BI	GAGCTC	4 cut(s)	540, 1164, 1500, 1540
Psp5II	RGGWCCY	2 cut(s)	1369, 1928
Psp6I	CCWGG	3 cut(s)	1399, 2163, 2263
PspEI	GGTNACC	1 cut(s)	458
PspGI	CCWGG	3 cut(s)	1399, 2163, 2263
PspN4I	GGNNCC	4 cut(s)	198, 611, 1888, 1929
PspPI	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
PspPPI	RGGWCCY	2 cut(s)	1369, 1928
PspXI	VCTCGAGB	1 cut(s)	317
PsuI	RGATCY	2 cut(s)	234, 609
PteI	GCGCGC	1 cut(s)	521
RsaI	GTAC	5 cut(s)	813, 1075, 1410, 1530, 1825
RsaNI	GTAC	5 cut(s)	812, 1074, 1409, 1529, 1824
RseI	CAYNNNNRTG	3 cut(s)	1046, 1250, 1523
SacI	GAGCTC	4 cut(s)	540, 1164, 1500, 1540
SapI	GCTCTTC	1 cut(s)	349
SaqAI	TTAA	6 cut(s)	86, 797, 881, 983, 1740, 1820
Sau3AI	GATC	7 cut(s)	234, 325, 330, 609, 763, 840, 1855
Sau96I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
ScaI	AGTACT	1 cut(s)	1825
SchI	GAGTC	4 cut(s)	1124, 1140, 1442, 1451
ScrFI	CCNGG	6 cut(s)	808, 823, 1401, 1940, 2165, 2265
SduI	GDGCHC	7 cut(s)	257, 540, 758, 1014, 1164, 1500, 1540
SexAI	ACCWGGT	1 cut(s)	2163
SfaNI	GCATC	6 cut(s)	534, 855, 1473, 1495, 2127, 2264
Sfr274I	CTCGAG	1 cut(s)	317
SfuI	TTCGAA	1 cut(s)	1375
SinI	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
SlaI	CTCGAG	1 cut(s)	317
SmiMI	CAYNNNNRTG	3 cut(s)	1046, 1250, 1523
SmlI	CTYRAG	3 cut(s)	140, 317, 1819
SmoI	CTYRAG	3 cut(s)	140, 317, 1819
SphI	GCATGC	1 cut(s)	2225
Sse9I	AATT	7 cut(s)	10, 1002, 1831, 1952, 2191, 2198, 2238
SspMI	CTAG	3 cut(s)	773, 803, 2042
SstI	GAGCTC	4 cut(s)	540, 1164, 1500, 1540
StyD4I	CCNGG	6 cut(s)	806, 821, 1399, 1938, 2163, 2263
TaaI	ACNGT	6 cut(s)	753, 816, 1508, 1717, 1981, 1993
TaiI	ACGT	2 cut(s)	1482, 1879
TaqI	TCGA	7 cut(s)	267, 318, 335, 1375, 1431, 1534, 1576
TaqII	GACCGA	1 cut(s)	1619
TasI	AATT	7 cut(s)	10, 1002, 1831, 1952, 2191, 2198, 2238
TatI	WGTACW	1 cut(s)	1823
TauI	GCSGC	7 cut(s)	123, 345, 1468, 1607, 1892, 2118, 2132
TfiI	GAWTC	6 cut(s)	269, 337, 886, 1195, 1377, 2081
Tru1I	TTAA	6 cut(s)	86, 797, 881, 983, 1740, 1820
Tru9I	TTAA	6 cut(s)	86, 797, 881, 983, 1740, 1820
TscAI	CASTG	6 cut(s)	352, 1063, 1131, 1272, 1845, 2091
TseFI	GTSAC	3 cut(s)	1636, 2107, 2259
TseI	GCWGC	8 cut(s)	578, 581, 584, 602, 908, 1869, 2051, 2185
Tsp45I	GTSAC	3 cut(s)	1636, 2107, 2259
TspGWI	ACGGA	3 cut(s)	262, 456, 2024
TspRI	CASTG	6 cut(s)	352, 1063, 1131, 1272, 1845, 2091
Van91I	CCANNNNNTGG	1 cut(s)	2215
Vha464I	CTTAAG	1 cut(s)	1819
VpaK11BI	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
VspI	ATTAAT	1 cut(s)	86
XapI	RAATTY	3 cut(s)	1002, 1952, 2191
XceI	RCATGY	1 cut(s)	2225
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1153
XhoI	CTCGAG	1 cut(s)	317
XspI	CTAG	3 cut(s)	773, 803, 2042
ZrmI	AGTACT	1 cut(s)	1825
Zsp2I	ATGCAT	2 cut(s)	1488, 2006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.