Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Forward (+)

12399900 .. 12404889

Gene Structure 4990 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G012610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGGAACCTATGGTTCTGAACCTAACCAGCATGAGAACTTCTTCAGATGGAGCTTGGCAGGGTGATAATCCTCTGAACCATGCCTTCCCATTGTTGATAGTTCAAACCACTCTGGTCCTCTTCATCAGTCGCTTCTTAGCCTTCATTCTCAAACCACTCCGCCAACCCAAGGTCATTGCCGAGATCTTAGGTGGGATTTTGCTAGGACCTTCTGCACTTGGCCTGATCAAAGATTTCTCCCCCATCATATTCCCTTCATGGAGCACTCCCATACTCGAATCAGTAGCAAGCATCGGCCTCCTCTTCTACCTATTCCTCGTAGGCCTCGAGCTCGACTTGCGCTCCATTCGCCGCAGTGGCAAAAGCGCCATCACCATAGCACTCGCAGGCATTTCCCTCCCTTTCCTCATCGCTGTAGGAGTCACATTCCTCATGCGCAAGGCCATCAACGGTGAGAACAAAGTAGGTTACCCTCAGTTCCTCGTGTTCATAGGGGTAGCCCTCTCCATCACCGCCTTCCCTGTCCTCGCACGCATCTTAGCAGAGCTCAAACTGCTCACCACAAGCATAGGTGAGACGGCAATGGCTGCAGCAGCCTTCAACGACGTGGCTGCATGGATTCTGCTGGCCTTGGCCGTGGCCTTAGCAGGTGGGAACCATAAAGGGCCTCTAGTCTCCATTTGGGTTCTCATTTCCGGCGTGGCTTTTGTTGCTTTTATGTTCATTCTGGTCCGTCCTTTGATGACTTGGGTTGCTAACCGCTGCTCATCTCAAATGGAGGACGAAGCTTATATATGCTTCACCCTAGCCGGGGTCATGTTGTCCGGGTTCATGACTGACCTAATCGGTGTCCATGCCATCTTTGGGGCATTTATTTTTGGATTAACCATTCCGAAAGGGGGTGAATTCGCAGCCAAACTGACTAAAAGGATCGAGGATTTTGTGTCTGGTTTGCTTCTCCCTCTCTACTTTGCCTCAAGTGGTTTGAAGACTGACGTTACTAAAATACGTGGGATCAAGGCATGGGGTCTTTTGGCATTGGTTATATCAGTTTCTTGCACTGGAAAAATTTTGGGAACGTTTTTGGTTGCAATGATGTGTATGATACCAGTGAGGGAGTCCTTAGCTCTAGGTGTACTGATGAATACTAAAGGATTGGTGGAGCTTGTTGTTCTGAATATTGGCAAGGAGAAAAAGATTCTAAACGACGAGATGTTTGCTATTTTTGTGCTGATGGCACTTTTCACCACCTTCATGACGACACCAATAGTGATGGCCACTTACAAACCCTATGTCGTTGCTTCCCAATCTGAGAGTAGTTTAGAGAGACAGAATTCTCCCAGCAAGTACCCGAAGAAAAAGAAGGAACTTCGAATCCTTGCTTGTATTCATGGACCGCGAAGTGTACCCTCCCTTATCAACCTCATTGAGTCGACCGGAACCTCCTCCGAGAATTCTCTTCTCAAAGTTTACGTGATGCATCTTGTTGAACTTACTGATCGTTCATCCTCCATTATGATGGTACACAAAGTTCGGAAAAACGGCTTGCCCTTCATCAACCGCTTTTGTCTAGGGGTGGCACGACATATCGCCTCAGCATTTGAAGCTTATAGTCAAGTTGGTCAAGTCACCATCCGTCACACAACAGCCATCTCGGCTTTGTCTACGATGCACGAGGATATTCGCCATGTTGCAGAAGCCAAGAGGGTGTCCATCATTGTCTTGCCTTTCAACAAACGGGGGAATAAACAGGGTGAAGAGACGATGGATAATTTAGAGCATCGCTGGAGAGCAGTGAATGAGAGAGTTCTAAAATGTGCTAATTGCTCAGTAGCATTGTTTGTGGATCGTGGACTCGGTGTTGGAGCTGAGAAGGGTGAAAAGACTACCAGTCTGAAGAGGGTGTGCATTCTATTCTTGGGTGGAACCGATGACCGCAGGGCACTGCAGTATGTTGCGGGAATTGCAAATCAGAGCGTTGTAGTTACCGTTGTGAGGTGTTTGGAGCACAGCACTACTCCTCCAAGCCCCATCACCAACCCCGACAGGGATCTTGATGAGACTGCAGTGGCAGAGTTTCTAAGGCAGAATGGGTCGGTGGAATTCATAGAGAAGGTGGTAGTCGGCAACATTATAGAAACGGTGGCGGGTATAGTTCAGGGCAGGGAATACAACCTGGTGATCGTGGGGAAGCAGCACCACCCTCCAACAATTGTTGCAAAATTGGCTCACAATCAGCTGGAGCATACCGAACTGGGACCAATTGGAGACGCATTAGCTTCATCCTCACATGGGATTTCGTGCTCCGTGCTTGTGATACAAACACCACAGATTAGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

780

Amino Acids

84.75

Weight (kDa)

9.22

Isoelectric Point (pI)

37.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	41 - 424	1e-62	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	488 - 624	2.5e-33	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	635 - 774	1.6e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	638
Acc16I	TGCGCA	1 cut(s)	437
Acc36I	ACCTGC	1 cut(s)	638
AccI	GTMKAC	2 cut(s)	1433, 1664
AccII	CGCG	1 cut(s)	1399
AciI	CCGC	9 cut(s)	160, 352, 513, 760, 1397, 1561, 1936, 1958, 2147
AclI	AACGTT	1 cut(s)	1079
AclWI	GGATC	4 cut(s)	938, 1022, 1854, 2058
AcoI	YGGCCR	2 cut(s)	633, 1275
AcsI	RAATTY	5 cut(s)	905, 1068, 1333, 1453, 2102
AcuI	CTGAAG	2 cut(s)	27, 1916
AfaI	GTAC	4 cut(s)	1137, 1349, 1407, 1524
AfiI	CCNNNNNNNGG	8 cut(s)	169, 810, 811, 864, 899, 1995, 2047, 2292
AgsI	TTSAA	6 cut(s)	104, 601, 988, 1490, 1604, 1732
AhdI	GACNNNNNGTC	1 cut(s)	1890
AjiI	CACGTC	1 cut(s)	607
AjnI	CCWGG	1 cut(s)	2175
AloI	GAACNNNNNNTCC	2 cut(s)	1359, 1391
Alw21I	GWGCWC	5 cut(s)	266, 333, 549, 2010, 2306
Alw26I	GTCTC	6 cut(s)	569, 679, 1321, 1754, 2054, 2262
AlwI	GGATC	4 cut(s)	938, 1022, 1854, 2058
Ama87I	CYCGRG	1 cut(s)	326
AoxI	GGCC	9 cut(s)	220, 295, 322, 441, 627, 633, 639, 665, 1275
ApeKI	GCWGC	7 cut(s)	587, 590, 593, 611, 762, 911, 2194
ApoI	RAATTY	5 cut(s)	905, 1068, 1333, 1453, 2102
ArsI	GACNNNNNNTTYG	4 cut(s)	1199, 1231, 1278, 1310
Asp700I	GAANNNNTTC	1 cut(s)	40
AspLEI	GCGC	3 cut(s)	342, 368, 438
AspS9I	GGNCC	6 cut(s)	115, 206, 665, 730, 1394, 2258
AsuC2I	CCSGG	2 cut(s)	811, 826
AsuII	TTCGAA	1 cut(s)	1372
AvaI	CYCGRG	1 cut(s)	326
AvaII	GGWCC	5 cut(s)	115, 206, 730, 1394, 2258
BaeGI	GKGCMC	1 cut(s)	1945
BalI	TGGCCA	1 cut(s)	1277
BanII	GRGCYC	2 cut(s)	333, 549
BauI	CACGAG	2 cut(s)	482, 1673
BbsI	GAAGAC	1 cut(s)	995
Bbv12I	GWGCWC	5 cut(s)	266, 333, 549, 2010, 2306
BbvCI	CCTCAGC	1 cut(s)	1594
BbvI	GCAGC	7 cut(s)	574, 598, 602, 605, 749, 923, 2206
BceAI	ACGGC	3 cut(s)	594, 620, 1558
BcgI	CGANNNNNNTGC	4 cut(s)	593, 627, 1199, 1233
BciT130I	CCWGG	1 cut(s)	2177
BclI	TGATCA	1 cut(s)	225
BcnI	CCSGG	2 cut(s)	811, 826
BcoDI	GTCTC	6 cut(s)	569, 679, 1321, 1754, 2054, 2262
BfaI	CTAG	5 cut(s)	203, 671, 806, 1130, 1571
BfmI	CTRYAG	4 cut(s)	414, 588, 1946, 2064
BfoI	RGCGCY	1 cut(s)	369
BfuAI	ACCTGC	1 cut(s)	638
BglI	GCCNNNNNGGC	2 cut(s)	357, 1655
BglII	AGATCT	1 cut(s)	183
BisI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2195
BlsI	GCNGC	8 cut(s)	353, 589, 592, 595, 613, 764, 913, 2196
Bme1390I	CCNGG	3 cut(s)	811, 826, 2177
Bme18I	GGWCC	5 cut(s)	115, 206, 730, 1394, 2258
BmeRI	GACNNNNNGTC	1 cut(s)	1890
BmeT110I	CYCGRG	1 cut(s)	326
BmgBI	CACGTC	1 cut(s)	607
BmgT120I	GGNCC	6 cut(s)	115, 206, 665, 730, 1394, 2258
BmiI	GGNNCC	5 cut(s)	6, 656, 1441, 1927, 2259
BmrFI	CCNGG	3 cut(s)	811, 826, 2177
BmrI	ACTGGG	1 cut(s)	2264
BmsI	GCATC	6 cut(s)	300, 543, 1467, 1489, 1659, 1789
BmuI	ACTGGG	1 cut(s)	2264
BpiI	GAAGAC	1 cut(s)	995
BpmI	CTGGAG	2 cut(s)	1807, 2261
Bpu10I	CCTNAGC	3 cut(s)	643, 1123, 1594
Bpu14I	TTCGAA	1 cut(s)	1372
BpuEI	CTTGAG	1 cut(s)	961
BpuMI	CCSGG	2 cut(s)	811, 826
BsaAI	YACGTR	2 cut(s)	1010, 1474
BsaJI	CCNNGG	4 cut(s)	168, 630, 636, 810
BsaWI	WCCGGW	1 cut(s)	1436
BsaXI	ACNNNNNCTCC	4 cut(s)	326, 356, 2005, 2035
Bsc4I	CCNNNNNNNGG	8 cut(s)	169, 810, 811, 864, 899, 1995, 2047, 2292
Bse1I	ACTGG	4 cut(s)	1066, 1109, 1890, 2259
Bse3DI	GCAATG	3 cut(s)	174, 588, 1098
BseBI	CCWGG	1 cut(s)	2177
BseDI	CCNNGG	4 cut(s)	168, 630, 636, 810
BseGI	GGATG	3 cut(s)	1505, 1632, 2282
BseLI	CCNNNNNNNGG	8 cut(s)	169, 810, 811, 864, 899, 1995, 2047, 2292
BseMI	GCAATG	3 cut(s)	174, 588, 1098
BseMII	CTCAG	5 cut(s)	488, 1302, 1608, 1842, 1860
BseNI	ACTGG	4 cut(s)	1066, 1109, 1890, 2259
BseRI	GAGGAG	3 cut(s)	290, 1435, 2010
BseSI	GKGCMC	1 cut(s)	1945
BseXI	GCAGC	7 cut(s)	574, 598, 602, 605, 749, 923, 2206
BseYI	CCCAGC	1 cut(s)	1340
BsgI	GTGCAG	1 cut(s)	198
Bsh1236I	CGCG	1 cut(s)	1399
Bsh1285I	CGRYCG	1 cut(s)	1437
BshFI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 667, 1277
BsiEI	CGRYCG	1 cut(s)	1437
BsiHKAI	GWGCWC	5 cut(s)	266, 333, 549, 2010, 2306
BsiHKCI	CYCGRG	1 cut(s)	326
BsiSI	CCGG	4 cut(s)	696, 810, 825, 1437
BslFI	GGGAC	1 cut(s)	2271
BslI	CCNNNNNNNGG	8 cut(s)	169, 810, 811, 864, 899, 1995, 2047, 2292
BsmAI	GTCTC	6 cut(s)	569, 679, 1321, 1754, 2054, 2262
BsmBI	CGTCTC	3 cut(s)	569, 1754, 2262
BsmFI	GGGAC	1 cut(s)	2271
BsmI	GAATGC	1 cut(s)	1908
BsnI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 667, 1277
BsoBI	CYCGRG	1 cut(s)	326
Bsp119I	TTCGAA	1 cut(s)	1372
Bsp1286I	GDGCHC	6 cut(s)	266, 333, 549, 1945, 2010, 2306
Bsp143I	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
BspACI	CCGC	9 cut(s)	160, 352, 513, 760, 1397, 1561, 1936, 1958, 2147
BspANI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 667, 1277
BspCNI	CTCAG	5 cut(s)	487, 1303, 1607, 1841, 1861
BspFNI	CGCG	1 cut(s)	1399
BspHI	TCATGA	2 cut(s)	831, 1254
BspLI	GGNNCC	5 cut(s)	6, 656, 1441, 1927, 2259
BspMAI	CTGCAG	3 cut(s)	592, 1950, 2068
BspMI	ACCTGC	1 cut(s)	638
BspPI	GGATC	4 cut(s)	938, 1022, 1854, 2058
BspT104I	TTCGAA	1 cut(s)	1372
BsrDI	GCAATG	3 cut(s)	174, 588, 1098
BsrI	ACTGG	4 cut(s)	1066, 1109, 1890, 2259
BssECI	CCNNGG	4 cut(s)	168, 630, 636, 810
BssMI	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
BssSI	CACGAG	2 cut(s)	482, 1673
BssT1I	CCWWGG	2 cut(s)	168, 630
Bst2BI	CACGAG	2 cut(s)	482, 1673
Bst2UI	CCWGG	1 cut(s)	2177
Bst4CI	ACNGT	3 cut(s)	452, 1990, 2143
Bst6I	CTCTTC	5 cut(s)	125, 308, 1464, 1752, 1892
BstAPI	GCANNNNNTGC	1 cut(s)	587
BstBAI	YACGTR	2 cut(s)	1010, 1474
BstBI	TTCGAA	1 cut(s)	1372
BstC8I	GCNNGC	5 cut(s)	289, 388, 532, 627, 1547
BstDSI	CCRYGG	1 cut(s)	636
BstEII	GGTNACC	1 cut(s)	467
BstF5I	GGATG	3 cut(s)	1505, 1632, 2282
BstFNI	CGCG	1 cut(s)	1399
BstH2I	RGCGCY	1 cut(s)	369
BstHHI	GCGC	3 cut(s)	342, 368, 438
BstKTI	GATC	8 cut(s)	186, 228, 933, 1017, 1501, 1849, 2053, 2184
BstMAI	GTCTC	6 cut(s)	569, 679, 1321, 1754, 2054, 2262
BstMBI	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
BstMCI	CGRYCG	1 cut(s)	1437
BstNI	CCWGG	1 cut(s)	2177
BstPI	GGTNACC	1 cut(s)	467
BstSCI	CCNGG	3 cut(s)	809, 824, 2175
BstSFI	CTRYAG	4 cut(s)	414, 588, 1946, 2064
BstSLI	GKGCMC	1 cut(s)	1945
BstUI	CGCG	1 cut(s)	1399
BstV1I	GCAGC	7 cut(s)	574, 598, 602, 605, 749, 923, 2206
BstV2I	GAAGAC	1 cut(s)	995
BstX2I	RGATCY	2 cut(s)	183, 2050
BstXI	CCANNNNNNTGG	1 cut(s)	1519
BstYI	RGATCY	2 cut(s)	183, 2050
BsuRI	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 667, 1277
BtgI	CCRYGG	1 cut(s)	636
BtgZI	GCGATG	2 cut(s)	394, 1766
BtrI	CACGTC	1 cut(s)	607
BtsCI	GGATG	3 cut(s)	1505, 1632, 2282
BtsI	GCAGTG	4 cut(s)	361, 1800, 1943, 2073
BtsIMutI	CAGTG	6 cut(s)	361, 1059, 1116, 1800, 1943, 2073
BveI	ACCTGC	1 cut(s)	638
Cac8I	GCNNGC	5 cut(s)	289, 388, 532, 627, 1547
CciI	TCATGA	2 cut(s)	831, 1254
CfoI	GCGC	3 cut(s)	342, 368, 438
Cfr13I	GGNCC	6 cut(s)	115, 206, 665, 730, 1394, 2258
CseI	GACGC	1 cut(s)	2279
CsiI	ACCWGGT	1 cut(s)	2175
Csp6I	GTAC	4 cut(s)	1136, 1348, 1406, 1523
CviQI	GTAC	4 cut(s)	1136, 1348, 1406, 1523
DpnI	GATC	8 cut(s)	185, 227, 932, 1016, 1500, 1848, 2052, 2183
DpnII	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
DriI	GACNNNNNGTC	1 cut(s)	1890
EaeI	YGGCCR	2 cut(s)	633, 1275
Eam1104I	CTCTTC	5 cut(s)	125, 308, 1464, 1752, 1892
Eam1105I	GACNNNNNGTC	1 cut(s)	1890
EarI	CTCTTC	5 cut(s)	125, 308, 1464, 1752, 1892
EciI	GGCGGA	1 cut(s)	149
Ecl136II	GAGCTC	2 cut(s)	331, 547
Eco130I	CCWWGG	2 cut(s)	168, 630
Eco147I	AGGCCT	1 cut(s)	324
Eco24I	GRGCYC	2 cut(s)	333, 549
Eco47I	GGWCC	5 cut(s)	115, 206, 730, 1394, 2258
Eco53kI	GAGCTC	2 cut(s)	331, 547
Eco57I	CTGAAG	2 cut(s)	27, 1916
Eco88I	CYCGRG	1 cut(s)	326
Eco91I	GGTNACC	1 cut(s)	467
EcoICRI	GAGCTC	2 cut(s)	331, 547
EcoO109I	RGGNCCY	2 cut(s)	206, 665
EcoO65I	GGTNACC	1 cut(s)	467
EcoRI	GAATTC	4 cut(s)	905, 1333, 1453, 2102
EcoRII	CCWGG	1 cut(s)	2175
EcoT14I	CCWWGG	2 cut(s)	168, 630
EcoT22I	ATGCAT	1 cut(s)	1482
EcoT38I	GRGCYC	2 cut(s)	333, 549
ErhI	CCWWGG	2 cut(s)	168, 630
Esp3I	CGTCTC	3 cut(s)	569, 1754, 2262
FaqI	GGGAC	1 cut(s)	2271
FauI	CCCGC	2 cut(s)	1951, 2140
FbaI	TGATCA	1 cut(s)	225
FblI	GTMKAC	2 cut(s)	1433, 1664
Fnu4HI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2195
FokI	GGATG	3 cut(s)	1492, 1619, 2269
FriOI	GRGCYC	2 cut(s)	333, 549
Fsp4HI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2195
FspBI	CTAG	5 cut(s)	203, 671, 806, 1130, 1571
FspI	TGCGCA	1 cut(s)	437
GlaI	GCGC	3 cut(s)	341, 367, 437
GluI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2195
GsaI	CCCAGC	1 cut(s)	1344
GsuI	CTGGAG	2 cut(s)	1807, 2261
HaeII	RGCGCY	1 cut(s)	369
HaeIII	GGCC	9 cut(s)	222, 297, 324, 443, 629, 635, 641, 667, 1277
HapII	CCGG	4 cut(s)	696, 810, 825, 1437
HgaI	GACGC	1 cut(s)	2279
HhaI	GCGC	3 cut(s)	342, 368, 438
Hin6I	GCGC	3 cut(s)	340, 366, 436
HinP1I	GCGC	3 cut(s)	340, 366, 436
HincII	GTYRAC	1 cut(s)	1434
HindII	GTYRAC	1 cut(s)	1434
HindIII	AAGCTT	2 cut(s)	786, 1605
HinfI	GANTC	8 cut(s)	278, 420, 619, 1118, 1198, 1374, 1430, 1854
HpaII	CCGG	4 cut(s)	696, 810, 825, 1437
Hpy166II	GTNNAC	7 cut(s)	1136, 1406, 1434, 1471, 1525, 1665, 1853
Hpy188III	TCNNGA	3 cut(s)	832, 1255, 2054
Hpy8I	GTNNAC	7 cut(s)	1136, 1406, 1434, 1471, 1525, 1665, 1853
Hpy99I	CGWCG	2 cut(s)	608, 1211
HpyCH4III	ACNGT	3 cut(s)	452, 1990, 2143
HpyCH4IV	ACGT	5 cut(s)	606, 996, 1009, 1079, 1473
HpySE526I	ACGT	5 cut(s)	606, 996, 1009, 1079, 1473
HspAI	GCGC	3 cut(s)	340, 366, 436
Ksp22I	TGATCA	1 cut(s)	225
Kzo9I	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
LmnI	GCTCC	8 cut(s)	50, 261, 347, 1162, 1865, 2005, 2242, 2309
Lsp1109I	GCAGC	7 cut(s)	574, 598, 602, 605, 749, 923, 2206
LweI	GCATC	6 cut(s)	300, 543, 1467, 1489, 1659, 1789
MabI	ACCWGGT	1 cut(s)	2175
MaeI	CTAG	5 cut(s)	203, 671, 806, 1130, 1571
MaeII	ACGT	5 cut(s)	606, 996, 1009, 1079, 1473
MaeIII	GTNAC	6 cut(s)	421, 467, 997, 1627, 1637, 1984
MalI	GATC	8 cut(s)	185, 227, 932, 1016, 1500, 1848, 2052, 2183
MboI	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
MboII	GAAGA	8 cut(s)	33, 112, 295, 1000, 1366, 1451, 1769, 1909
MfeI	CAATTG	2 cut(s)	2211, 2262
MflI	RGATCY	2 cut(s)	183, 2050
MhlI	GDGCHC	6 cut(s)	266, 333, 549, 1945, 2010, 2306
MlsI	TGGCCA	1 cut(s)	1277
MluNI	TGGCCA	1 cut(s)	1277
MlyI	GAGTC	4 cut(s)	429, 1127, 1439, 1848
MmeI	TCCRAC	2 cut(s)	1843, 2231
Mox20I	TGGCCA	1 cut(s)	1277
Mph1103I	ATGCAT	1 cut(s)	1482
MroXI	GAANNNNTTC	1 cut(s)	40
MscI	TGGCCA	1 cut(s)	1277
MseI	TTAA	1 cut(s)	884
MslI	CAYNNNNRTG	2 cut(s)	1253, 1517
Msp20I	TGGCCA	1 cut(s)	1277
MspA1I	CMGCKG	2 cut(s)	762, 2239
MspI	CCGG	4 cut(s)	696, 810, 825, 1437
MspR9I	CCNGG	3 cut(s)	811, 826, 2177
MunI	CAATTG	2 cut(s)	2211, 2262
Mva1269I	GAATGC	1 cut(s)	1908
MvaI	CCWGG	1 cut(s)	2177
MvnI	CGCG	1 cut(s)	1399
NciI	CCSGG	2 cut(s)	811, 826
NdeII	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
NlaIV	GGNNCC	5 cut(s)	6, 656, 1441, 1927, 2259
NmeAIII	GCCGAG	2 cut(s)	205, 1634
NmuCI	GTSAC	3 cut(s)	421, 1627, 1637
NsbI	TGCGCA	1 cut(s)	437
NsiI	ATGCAT	1 cut(s)	1482
NspV	TTCGAA	1 cut(s)	1372
PaeR7I	CTCGAG	1 cut(s)	326
PagI	TCATGA	2 cut(s)	831, 1254
PaqCI	CACCTGC	1 cut(s)	638
PceI	AGGCCT	1 cut(s)	324
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PctI	GAATGC	1 cut(s)	1908
PdmI	GAANNNNTTC	1 cut(s)	40
PfeI	GAWTC	4 cut(s)	278, 619, 1198, 1374
PkrI	GCNGC	8 cut(s)	353, 589, 592, 595, 613, 764, 913, 2196
PleI	GAGTC	4 cut(s)	428, 1126, 1438, 1848
PpsI	GAGTC	4 cut(s)	428, 1126, 1438, 1848
Ppu21I	YACGTR	2 cut(s)	1010, 1474
PpuMI	RGGWCCY	1 cut(s)	206
Psp124BI	GAGCTC	2 cut(s)	333, 549
Psp1406I	AACGTT	1 cut(s)	1079
Psp5II	RGGWCCY	1 cut(s)	206
Psp6I	CCWGG	1 cut(s)	2175
PspEI	GGTNACC	1 cut(s)	467
PspFI	CCCAGC	1 cut(s)	1340
PspGI	CCWGG	1 cut(s)	2175
PspN4I	GGNNCC	5 cut(s)	6, 656, 1441, 1927, 2259
PspPI	GGNCC	6 cut(s)	115, 206, 665, 730, 1394, 2258
PspPPI	RGGWCCY	1 cut(s)	206
PspXI	VCTCGAGB	1 cut(s)	326
PsrI	GAACNNNNNNTAC	2 cut(s)	1515, 1547
PstI	CTGCAG	3 cut(s)	592, 1950, 2068
PsuI	RGATCY	2 cut(s)	183, 2050
PvuII	CAGCTG	1 cut(s)	2239
RsaI	GTAC	4 cut(s)	1137, 1349, 1407, 1524
RsaNI	GTAC	4 cut(s)	1136, 1348, 1406, 1523
RseI	CAYNNNNRTG	2 cut(s)	1253, 1517
SacI	GAGCTC	2 cut(s)	333, 549
SalI	GTCGAC	1 cut(s)	1432
SaqAI	TTAA	1 cut(s)	884
SatI	GCNGC	8 cut(s)	352, 588, 591, 594, 612, 763, 912, 2195
Sau3AI	GATC	8 cut(s)	183, 225, 930, 1014, 1498, 1846, 2050, 2181
Sau96I	GGNCC	6 cut(s)	115, 206, 665, 730, 1394, 2258
SchI	GAGTC	4 cut(s)	429, 1127, 1439, 1848
ScrFI	CCNGG	3 cut(s)	811, 826, 2177
SduI	GDGCHC	6 cut(s)	266, 333, 549, 1945, 2010, 2306
SexAI	ACCWGGT	1 cut(s)	2175
SfaNI	GCATC	6 cut(s)	300, 543, 1467, 1489, 1659, 1789
SfcI	CTRYAG	4 cut(s)	414, 588, 1946, 2064
Sfr274I	CTCGAG	1 cut(s)	326
SfuI	TTCGAA	1 cut(s)	1372
SinI	GGWCC	5 cut(s)	115, 206, 730, 1394, 2258
SlaI	CTCGAG	1 cut(s)	326
SmiMI	CAYNNNNRTG	2 cut(s)	1253, 1517
SmlI	CTYRAG	2 cut(s)	326, 976
SmoI	CTYRAG	2 cut(s)	326, 976
SseBI	AGGCCT	1 cut(s)	324
SsiI	CCGC	9 cut(s)	160, 352, 513, 760, 1397, 1561, 1936, 1958, 2147
SspI	AATATT	1 cut(s)	1180
SspMI	CTAG	5 cut(s)	203, 671, 806, 1130, 1571
SstI	GAGCTC	2 cut(s)	333, 549
StuI	AGGCCT	1 cut(s)	324
StyD4I	CCNGG	3 cut(s)	809, 824, 2175
StyI	CCWWGG	2 cut(s)	168, 630
TaaI	ACNGT	3 cut(s)	452, 1990, 2143
TaiI	ACGT	5 cut(s)	609, 999, 1012, 1082, 1476
TaqI	TCGA	6 cut(s)	276, 327, 333, 933, 1372, 1433
TatI	WGTACW	1 cut(s)	1135
TauI	GCSGC	1 cut(s)	354
TfiI	GAWTC	4 cut(s)	278, 619, 1198, 1374
Tru1I	TTAA	1 cut(s)	884
Tru9I	TTAA	1 cut(s)	884
TscAI	CASTG	6 cut(s)	361, 1066, 1116, 1800, 1950, 2073
TseFI	GTSAC	3 cut(s)	421, 1627, 1637
TseI	GCWGC	7 cut(s)	587, 590, 593, 611, 762, 911, 2194
Tsp45I	GTSAC	3 cut(s)	421, 1627, 1637
TspGWI	ACGGA	3 cut(s)	722, 1625, 2296
TspRI	CASTG	6 cut(s)	361, 1066, 1116, 1800, 1950, 2073
VpaK11BI	GGWCC	5 cut(s)	115, 206, 730, 1394, 2258
XapI	RAATTY	5 cut(s)	905, 1068, 1333, 1453, 2102
XcmI	CCANNNNNNNNNTGG	1 cut(s)	860
XhoI	CTCGAG	1 cut(s)	326
XmiI	GTMKAC	2 cut(s)	1433, 1664
XmnI	GAANNNNTTC	1 cut(s)	40
XspI	CTAG	5 cut(s)	203, 671, 806, 1130, 1571
Zsp2I	ATGCAT	1 cut(s)	1482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.