Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Reverse (-)

15886049 .. 15889882

Gene Structure 3834 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g13420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1791 bp

ATGAACTGGGTGGCCCGCCGGAGCTCCCCAGAACAAGATGACGTAGACGAAGCCTATATATGCCTAACCCTAGCCGGAGTCATGGTATCCGGATTCATTACTGACCTAATTGGTATCCATTCTATATTTGGAGCCTTTGTCTTTGGTCTAACAATTCCCAAAGAAGGAGAGTTTGCAGACAGATTGATGGCGAAGACTGAGGACTTTGTGTCCGGCTTGTTGCTTCCACTTTACTTTGCATCAAGTGGGTTGAAGACCGATGTAGGAAAGATACACGGTGCAGAGGCTTGGGGGCTGTTGGTGCTCGTTATATCGATCGCTTGTGCCGGGAAGATTTTGGGGACGTTTGTGGTGGCAATGACGTTTATGATTCCGGCTAGAGAATCGTTGACGCTTGGTGTGCTCATGAACACCAAAGGGTTGGTGGAGCTCATTGTTCTCAACATTGGCAAGGAGAAAAAGGTGCTTAATGATGAGACATTTGCTATTCTGGTAATCATGGCACTCTTCACCACCTTTATCACTAGTCCATTAGTGATGGCCATTTACAAGCCCGCACGTGGCATCTCAGTTCGCGCTCACCGTAAGCTCTGTGATCTGTCAACCAGTGAATCCTTCAAGGACGAGCTTCGAGTCCTGGCGTGTGTTCATGGGCCTGCCAATGTGCCCTCACTCATTAGCCTCATTGAGTCCATCCGAAGTGACAAGAAAGCCCAACTCAAACTCTTCCTCATGCACCTTGTGGAGCTAACTGAGGATCGATCTTCTTCCATCATCATGGTCCAACGGGCCCGAAAGAATGGCTTTCCCTTCTTTAAACGATTGGGCCGCTGCCCATGGAGCCAGGGCGTTGTTTCCGCCTTCAAGGCCTATAGCCAATTGGGCCGAGTATCGGTCCGACCCACAACCGCAATTTCAACCCTGTCCACAATGCACGAGGATGTTTGCCATGTGGCGGACGACCGGAGGGTGACGATGATCATCTTGCCATTCCACAAACAATGGATGCGTGAGAGCGATAATCATGAAGCGACGGAGGTTGTTGGCCACGGCTGGCGAGGGGTCAACCAGAGGGTGCTTCAAACTGCGCCGTGCTCCGTTGCAGTGCTCATTGATCGGGGGTTTGGTAACACCAGAGCCCAAACTCCAAGGCCTAGCACCACTGTGACCCGACGAGTTTGCATTGTGTTTTTTGGTGGGCCTGATGATCGCGAGGCCTTGGAGTTTGGCGGTAGGATGGCCGAACATCCGACAGTTAAAGTGGTGGTTGTGAAATTTGTTGAGAAAGAGGGCATGGAGAGTAATGGGATCATGTTAAAGCCTTCGCCCTCCAAGTCTACCGAGAATAGCTATTGTTTTTCCACAGCTAAAATGGACCGAAAGAAAGAAAAGGAGCTGGATGAGGAAGCAGTGGCTGAGTTCAGAAGCAAGTGGGATGGGAAAGCAGAGTACATAGAGAAGGTGGCAGCCAATAACGTTGTGGAGGGGGTTTTGGAAATAGGAAGAAGTGGAGATCATGACCTGTTAATCGTAGGCAAAGGAAGATTCCCGTCACGCATGGTGGCAGAATTGGCCGACCGCCATGCAGAGCATGCAGAGTTAGGCCCCATTGGAGACATATTGGCTTCATCGAGCCAGGGTGTGGTCTCCTCAGTGCTTGTGATTCAACAACATGACGTAGCTCATGCCGTGGAGGCTCCGGTGTCGAAGGTACTGCACGGTGATGACGAAAGGTTCGTCGCGGATGAGTCCTCCAGTTCTGTGCTTGAGATCTCTAAGGAATCTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

597

Amino Acids

65.43

Weight (kDa)

6.52

Isoelectric Point (pI)

39.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	6 - 179	1.1e-29	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	239 - 378	1.4e-34	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	393 - 558	6.4e-15	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1642
AccI	GTMKAC	2 cut(s)	45, 1337
AccII	CGCG	3 cut(s)	576, 1212, 1742
AccIII	TCCGGA	1 cut(s)	89
AciI	CCGC	9 cut(s)	16, 555, 829, 858, 909, 956, 1230, 1579, 1742
AclI	AACGTT	1 cut(s)	1476
AclWI	GGATC	2 cut(s)	765, 1316
AcoI	YGGCCR	4 cut(s)	540, 1045, 1239, 1572
AcsI	RAATTY	1 cut(s)	1274
AcvI	CACGTG	1 cut(s)	560
AdeI	CACNNNGTG	1 cut(s)	742
AfaI	GTAC	2 cut(s)	1451, 1713
AfiI	CCNNNNNNNGG	5 cut(s)	164, 560, 892, 955, 1642
AgsI	TTSAA	6 cut(s)	253, 619, 865, 918, 1082, 1667
AhdI	GACNNNNNGTC	1 cut(s)	208
AhlI	ACTAGT	1 cut(s)	524
AjnI	CCWGG	3 cut(s)	636, 843, 1635
AleI	CACNNNNGTG	1 cut(s)	1163
AluBI	AGCT	9 cut(s)	24, 430, 589, 628, 748, 1350, 1367, 1396, 1682
AluI	AGCT	9 cut(s)	24, 430, 589, 628, 748, 1350, 1367, 1396, 1682
Alw21I	GWGCWC	6 cut(s)	26, 306, 405, 432, 1097, 1110
Alw26I	GTCTC	3 cut(s)	470, 1608, 1651
AlwI	GGATC	2 cut(s)	765, 1316
AlwNI	CAGNNNCTG	1 cut(s)	1415
Aor13HI	TCCGGA	1 cut(s)	89
ApaI	GGGCCC	1 cut(s)	793
ApeKI	GCWGC	2 cut(s)	831, 1466
ApoI	RAATTY	1 cut(s)	1274
ArsI	GACNNNNNNTTYG	4 cut(s)	1718, 1722, 1750, 1754
AspLEI	GCGC	2 cut(s)	578, 1090
AsuC2I	CCSGG	1 cut(s)	328
AsuHPI	GGTGA	4 cut(s)	502, 572, 982, 1733
AvaII	GGWCC	3 cut(s)	781, 895, 1375
BaeGI	GKGCMC	2 cut(s)	669, 793
BalI	TGGCCA	2 cut(s)	542, 1047
BanII	GRGCYC	4 cut(s)	26, 432, 793, 1141
BauI	CACGAG	1 cut(s)	935
BbrPI	CACGTG	1 cut(s)	560
BbsI	GAAGAC	2 cut(s)	200, 260
Bbv12I	GWGCWC	6 cut(s)	26, 306, 405, 432, 1097, 1110
BbvI	GCAGC	2 cut(s)	818, 1478
BccI	CCATC	6 cut(s)	181, 532, 701, 779, 1231, 1430
BceAI	ACGGC	3 cut(s)	1066, 1075, 1673
BcgI	CGANNNNNNTGC	6 cut(s)	1161, 1195, 1565, 1599, 1696, 1730
BciT130I	CCWGG	3 cut(s)	638, 845, 1637
BciVI	GTATCC	2 cut(s)	97, 125
BclI	TGATCA	1 cut(s)	978
BcnI	CCSGG	1 cut(s)	328
BcoDI	GTCTC	3 cut(s)	470, 1608, 1651
BcuI	ACTAGT	1 cut(s)	524
BfaI	CTAG	4 cut(s)	71, 378, 525, 1155
BfmI	CTRYAG	1 cut(s)	871
BfuI	GTATCC	2 cut(s)	97, 125
BglI	GCCNNNNNGGC	3 cut(s)	866, 882, 1694
BglII	AGATCT	1 cut(s)	1770
BisI	GCNGC	3 cut(s)	829, 832, 1467
BlsI	GCNGC	3 cut(s)	830, 833, 1468
Bme1390I	CCNGG	4 cut(s)	328, 638, 845, 1637
Bme18I	GGWCC	3 cut(s)	781, 895, 1375
BmeRI	GACNNNNNGTC	1 cut(s)	208
BmiI	GGNNCC	5 cut(s)	133, 791, 842, 1606, 1698
BmrFI	CCNGG	4 cut(s)	328, 638, 845, 1637
BmrI	ACTGGG	1 cut(s)	16
BmsI	GCATC	3 cut(s)	248, 573, 996
BmuI	ACTGGG	1 cut(s)	16
BpiI	GAAGAC	2 cut(s)	200, 260
BpmI	CTGGAG	1 cut(s)	1738
BpuEI	CTTGAG	1 cut(s)	1787
BpuMI	CCSGG	1 cut(s)	328
Bsa29I	ATCGAT	2 cut(s)	314, 760
BsaAI	YACGTR	1 cut(s)	560
BsaBI	GATNNNNATC	1 cut(s)	980
BsaI	GGTCTC	1 cut(s)	1651
BsaJI	CCNNGG	7 cut(s)	836, 844, 1048, 1148, 1218, 1636, 1689
BsaWI	WCCGGW	3 cut(s)	89, 963, 1699
BsaXI	ACNNNNNCTCC	4 cut(s)	69, 99, 123, 153
Bsc4I	CCNNNNNNNGG	5 cut(s)	164, 560, 892, 955, 1642
Bse1I	ACTGG	3 cut(s)	11, 606, 1755
Bse3DI	GCAATG	1 cut(s)	363
Bse8I	GATNNNNATC	1 cut(s)	980
BseAI	TCCGGA	1 cut(s)	89
BseBI	CCWGG	3 cut(s)	638, 845, 1637
BseCI	ATCGAT	2 cut(s)	314, 760
BseDI	CCNNGG	7 cut(s)	836, 844, 1048, 1148, 1218, 1636, 1689
BseGI	GGATG	8 cut(s)	693, 946, 1011, 1242, 1246, 1405, 1441, 1750
BseJI	GATNNNNATC	1 cut(s)	980
BseLI	CCNNNNNNNGG	5 cut(s)	164, 560, 892, 955, 1642
BseMI	GCAATG	1 cut(s)	363
BseMII	CTCAG	5 cut(s)	189, 582, 744, 1407, 1665
BseNI	ACTGG	3 cut(s)	11, 606, 1755
BseRI	GAGGAG	1 cut(s)	1639
BseSI	GKGCMC	2 cut(s)	669, 793
BseXI	GCAGC	2 cut(s)	818, 1478
BsgI	GTGCAG	2 cut(s)	300, 1700
Bsh1236I	CGCG	3 cut(s)	576, 1212, 1742
Bsh1285I	CGRYCG	3 cut(s)	318, 964, 1579
BshVI	ATCGAT	2 cut(s)	314, 760
BsiEI	CGRYCG	3 cut(s)	318, 964, 1579
BsiHKAI	GWGCWC	6 cut(s)	26, 306, 405, 432, 1097, 1110
BsiSI	CCGG	8 cut(s)	19, 75, 90, 213, 327, 374, 964, 1700
BslFI	GGGAC	1 cut(s)	355
BslI	CCNNNNNNNGG	5 cut(s)	164, 560, 892, 955, 1642
BsmAI	GTCTC	3 cut(s)	470, 1608, 1651
BsmFI	GGGAC	1 cut(s)	355
Bso31I	GGTCTC	1 cut(s)	1651
Bsp120I	GGGCCC	1 cut(s)	789
Bsp1286I	GDGCHC	9 cut(s)	26, 306, 405, 432, 669, 793, 1097, 1110, 1141
Bsp13I	TCCGGA	1 cut(s)	89
Bsp19I	CCATGG	1 cut(s)	836
Bsp68I	TCGCGA	1 cut(s)	1212
BspACI	CCGC	9 cut(s)	16, 555, 829, 858, 909, 956, 1230, 1579, 1742
BspCNI	CTCAG	5 cut(s)	190, 581, 745, 1408, 1664
BspDI	ATCGAT	2 cut(s)	314, 760
BspEI	TCCGGA	1 cut(s)	89
BspFNI	CGCG	3 cut(s)	576, 1212, 1742
BspHI	TCATGA	3 cut(s)	405, 1024, 1516
BspLI	GGNNCC	5 cut(s)	133, 791, 842, 1606, 1698
BspPI	GGATC	2 cut(s)	765, 1316
BspTNI	GGTCTC	1 cut(s)	1651
BsrDI	GCAATG	1 cut(s)	363
BsrI	ACTGG	3 cut(s)	11, 606, 1755
BssECI	CCNNGG	7 cut(s)	836, 844, 1048, 1148, 1218, 1636, 1689
BssSI	CACGAG	1 cut(s)	935
BssT1I	CCWWGG	3 cut(s)	836, 1148, 1218
Bst2BI	CACGAG	1 cut(s)	935
Bst2UI	CCWGG	3 cut(s)	638, 845, 1637
Bst4CI	ACNGT	5 cut(s)	278, 584, 1165, 1255, 1721
Bst6I	CTCTTC	2 cut(s)	512, 731
BstAPI	GCANNNNNTGC	1 cut(s)	1592
BstBAI	YACGTR	1 cut(s)	560
BstC8I	GCNNGC	5 cut(s)	16, 555, 657, 1055, 1593
BstDEI	CTNAG	6 cut(s)	198, 568, 753, 1416, 1651, 1776
BstDSI	CCRYGG	3 cut(s)	836, 1048, 1689
BstF5I	GGATG	8 cut(s)	693, 946, 1011, 1242, 1246, 1405, 1441, 1750
BstFNI	CGCG	3 cut(s)	576, 1212, 1742
BstHHI	GCGC	2 cut(s)	578, 1090
BstMAI	GTCTC	3 cut(s)	470, 1608, 1651
BstMCI	CGRYCG	3 cut(s)	318, 964, 1579
BstMWI	GCNNNNNNNGC	8 cut(s)	301, 400, 840, 866, 882, 1571, 1592, 1694
BstNI	CCWGG	3 cut(s)	638, 845, 1637
BstNSI	RCATGY	1 cut(s)	1595
BstSCI	CCNGG	4 cut(s)	326, 636, 843, 1635
BstSFI	CTRYAG	1 cut(s)	871
BstSLI	GKGCMC	2 cut(s)	669, 793
BstUI	CGCG	3 cut(s)	576, 1212, 1742
BstV1I	GCAGC	2 cut(s)	818, 1478
BstV2I	GAAGAC	2 cut(s)	200, 260
BstX2I	RGATCY	1 cut(s)	1770
BstXI	CCANNNNNNTGG	2 cut(s)	421, 778
BstYI	RGATCY	1 cut(s)	1770
Bsu15I	ATCGAT	2 cut(s)	314, 760
BsuI	GTATCC	2 cut(s)	97, 125
BsuTUI	ATCGAT	2 cut(s)	314, 760
BtgI	CCRYGG	3 cut(s)	836, 1048, 1689
BtsCI	GGATG	8 cut(s)	693, 946, 1011, 1242, 1246, 1405, 1441, 1750
BtsI	GCAGTG	2 cut(s)	1110, 1416
BtsIMutI	CAGTG	5 cut(s)	613, 1110, 1161, 1416, 1659
BtuMI	TCGCGA	1 cut(s)	1212
Cac8I	GCNNGC	5 cut(s)	16, 555, 657, 1055, 1593
CaiI	CAGNNNCTG	1 cut(s)	1415
CciI	TCATGA	3 cut(s)	405, 1024, 1516
CfoI	GCGC	2 cut(s)	578, 1090
ClaI	ATCGAT	2 cut(s)	314, 760
CpoI	CGGWCCG	1 cut(s)	895
CseI	GACGC	1 cut(s)	400
Csp6I	GTAC	2 cut(s)	1450, 1712
CspI	CGGWCCG	1 cut(s)	895
CviQI	GTAC	2 cut(s)	1450, 1712
DdeI	CTNAG	6 cut(s)	198, 568, 753, 1416, 1651, 1776
DraI	TTTAAA	1 cut(s)	817
DraIII	CACNNNGTG	1 cut(s)	742
DriI	GACNNNNNGTC	1 cut(s)	208
EaeI	YGGCCR	4 cut(s)	540, 1045, 1239, 1572
Eam1104I	CTCTTC	2 cut(s)	512, 731
Eam1105I	GACNNNNNGTC	1 cut(s)	208
EarI	CTCTTC	2 cut(s)	512, 731
EciI	GGCGGA	2 cut(s)	847, 971
Ecl136II	GAGCTC	2 cut(s)	24, 430
Eco130I	CCWWGG	3 cut(s)	836, 1148, 1218
Eco147I	AGGCCT	3 cut(s)	869, 1153, 1217
Eco24I	GRGCYC	4 cut(s)	26, 432, 793, 1141
Eco31I	GGTCTC	1 cut(s)	1651
Eco47I	GGWCC	3 cut(s)	781, 895, 1375
Eco53kI	GAGCTC	2 cut(s)	24, 430
Eco72I	CACGTG	1 cut(s)	560
EcoICRI	GAGCTC	2 cut(s)	24, 430
EcoO109I	RGGNCCY	1 cut(s)	1604
EcoRII	CCWGG	3 cut(s)	636, 843, 1635
EcoT14I	CCWWGG	3 cut(s)	836, 1148, 1218
EcoT38I	GRGCYC	4 cut(s)	26, 432, 793, 1141
ErhI	CCWWGG	3 cut(s)	836, 1148, 1218
FalI	AAGNNNNNCTT	2 cut(s)	788, 820
FaqI	GGGAC	1 cut(s)	355
FauI	CCCGC	2 cut(s)	23, 562
FbaI	TGATCA	1 cut(s)	978
FblI	GTMKAC	2 cut(s)	45, 1337
Fnu4HI	GCNGC	3 cut(s)	829, 832, 1467
FokI	GGATG	8 cut(s)	680, 953, 1018, 1233, 1249, 1412, 1448, 1757
FriOI	GRGCYC	4 cut(s)	26, 432, 793, 1141
Fsp4HI	GCNGC	3 cut(s)	829, 832, 1467
FspBI	CTAG	4 cut(s)	71, 378, 525, 1155
GlaI	GCGC	2 cut(s)	577, 1089
GluI	GCNGC	3 cut(s)	829, 832, 1467
GsuI	CTGGAG	1 cut(s)	1738
HapII	CCGG	8 cut(s)	19, 75, 90, 213, 327, 374, 964, 1700
HgaI	GACGC	1 cut(s)	400
HhaI	GCGC	2 cut(s)	578, 1090
Hin6I	GCGC	2 cut(s)	576, 1088
HinP1I	GCGC	2 cut(s)	576, 1088
HincII	GTYRAC	3 cut(s)	390, 603, 1066
HindII	GTYRAC	3 cut(s)	390, 603, 1066
HpaII	CCGG	8 cut(s)	19, 75, 90, 213, 327, 374, 964, 1700
HphI	GGTGA	4 cut(s)	502, 572, 982, 1733
Hpy166II	GTNNAC	6 cut(s)	46, 390, 603, 927, 1066, 1338
Hpy188I	TCNGA	5 cut(s)	698, 899, 1251, 1424, 1790
Hpy188III	TCNNGA	5 cut(s)	90, 406, 1025, 1211, 1517
Hpy8I	GTNNAC	6 cut(s)	46, 390, 603, 927, 1066, 1338
Hpy99I	CGWCG	3 cut(s)	1036, 1176, 1742
HpyAV	CCTTC	7 cut(s)	158, 625, 820, 871, 1332, 1453, 1702
HpyCH4III	ACNGT	5 cut(s)	278, 584, 1165, 1255, 1721
HpyCH4IV	ACGT	6 cut(s)	42, 344, 362, 559, 1476, 1677
HpyF10VI	GCNNNNNNNGC	8 cut(s)	301, 400, 840, 866, 882, 1571, 1592, 1694
HpyF3I	CTNAG	6 cut(s)	198, 568, 753, 1416, 1651, 1776
HpySE526I	ACGT	6 cut(s)	42, 344, 362, 559, 1476, 1677
HspAI	GCGC	2 cut(s)	576, 1088
Kpn2I	TCCGGA	1 cut(s)	89
Ksp22I	TGATCA	1 cut(s)	978
LmnI	GCTCC	9 cut(s)	21, 29, 131, 427, 745, 840, 1100, 1393, 1702
Lsp1109I	GCAGC	2 cut(s)	818, 1478
LweI	GCATC	3 cut(s)	248, 573, 996
MaeI	CTAG	4 cut(s)	71, 378, 525, 1155
MaeII	ACGT	6 cut(s)	42, 344, 362, 559, 1476, 1677
MaeIII	GTNAC	5 cut(s)	701, 970, 1127, 1165, 1551
MboII	GAAGA	9 cut(s)	205, 265, 343, 499, 718, 756, 759, 1515, 1554
MfeI	CAATTG	1 cut(s)	878
MflI	RGATCY	1 cut(s)	1770
MhlI	GDGCHC	9 cut(s)	26, 306, 405, 432, 669, 793, 1097, 1110, 1141
MlsI	TGGCCA	2 cut(s)	542, 1047
MluCI	AATT	6 cut(s)	108, 153, 878, 912, 1274, 1568
MluNI	TGGCCA	2 cut(s)	542, 1047
MlyI	GAGTC	4 cut(s)	87, 642, 698, 1757
MmeI	TCCRAC	3 cut(s)	808, 922, 1274
Mox20I	TGGCCA	2 cut(s)	542, 1047
MroI	TCCGGA	1 cut(s)	89
MscI	TGGCCA	2 cut(s)	542, 1047
MseI	TTAA	5 cut(s)	468, 816, 1257, 1316, 1526
MslI	CAYNNNNRTG	4 cut(s)	776, 939, 1163, 1722
Msp20I	TGGCCA	2 cut(s)	542, 1047
MspA1I	CMGCKG	1 cut(s)	831
MspI	CCGG	8 cut(s)	19, 75, 90, 213, 327, 374, 964, 1700
MspR9I	CCNGG	4 cut(s)	328, 638, 845, 1637
MunI	CAATTG	1 cut(s)	878
MvaI	CCWGG	3 cut(s)	638, 845, 1637
MvnI	CGCG	3 cut(s)	576, 1212, 1742
MwoI	GCNNNNNNNGC	8 cut(s)	301, 400, 840, 866, 882, 1571, 1592, 1694
NciI	CCSGG	1 cut(s)	328
NcoI	CCATGG	1 cut(s)	836
NlaIV	GGNNCC	5 cut(s)	133, 791, 842, 1606, 1698
NmeAIII	GCCGAG	1 cut(s)	911
NmuCI	GTSAC	4 cut(s)	701, 970, 1165, 1551
NruI	TCGCGA	1 cut(s)	1212
NspI	RCATGY	1 cut(s)	1595
OliI	CACNNNNGTG	1 cut(s)	1163
PaeI	GCATGC	1 cut(s)	1595
PagI	TCATGA	3 cut(s)	405, 1024, 1516
PceI	AGGCCT	3 cut(s)	869, 1153, 1217
PcsI	WCGNNNNNNNCGW	3 cut(s)	580, 1725, 1734
PfeI	GAWTC	7 cut(s)	93, 370, 383, 611, 1545, 1663, 1781
PflMI	CCANNNNNTGG	1 cut(s)	1642
PkrI	GCNGC	3 cut(s)	830, 833, 1468
Ple19I	CGATCG	1 cut(s)	318
PleI	GAGTC	4 cut(s)	86, 641, 697, 1756
PmaCI	CACGTG	1 cut(s)	560
PmlI	CACGTG	1 cut(s)	560
PpsI	GAGTC	4 cut(s)	86, 641, 697, 1756
Ppu21I	YACGTR	1 cut(s)	560
Psp124BI	GAGCTC	2 cut(s)	26, 432
Psp1406I	AACGTT	1 cut(s)	1476
Psp6I	CCWGG	3 cut(s)	636, 843, 1635
PspCI	CACGTG	1 cut(s)	560
PspGI	CCWGG	3 cut(s)	636, 843, 1635
PspN4I	GGNNCC	5 cut(s)	133, 791, 842, 1606, 1698
PspOMI	GGGCCC	1 cut(s)	789
PstNI	CAGNNNCTG	1 cut(s)	1415
PsuI	RGATCY	1 cut(s)	1770
PvuI	CGATCG	1 cut(s)	318
RruI	TCGCGA	1 cut(s)	1212
RsaI	GTAC	2 cut(s)	1451, 1713
RsaNI	GTAC	2 cut(s)	1450, 1712
RseI	CAYNNNNRTG	4 cut(s)	776, 939, 1163, 1722
Rsr2I	CGGWCCG	1 cut(s)	895
RsrII	CGGWCCG	1 cut(s)	895
SacI	GAGCTC	2 cut(s)	26, 432
SaqAI	TTAA	5 cut(s)	468, 816, 1257, 1316, 1526
SatI	GCNGC	3 cut(s)	829, 832, 1467
SchI	GAGTC	4 cut(s)	87, 642, 698, 1757
ScrFI	CCNGG	4 cut(s)	328, 638, 845, 1637
SduI	GDGCHC	9 cut(s)	26, 306, 405, 432, 669, 793, 1097, 1110, 1141
SfaNI	GCATC	3 cut(s)	248, 573, 996
SfcI	CTRYAG	1 cut(s)	871
SinI	GGWCC	3 cut(s)	781, 895, 1375
SmiMI	CAYNNNNRTG	4 cut(s)	776, 939, 1163, 1722
SmlI	CTYRAG	1 cut(s)	1766
SmoI	CTYRAG	1 cut(s)	1766
SpeI	ACTAGT	1 cut(s)	524
SphI	GCATGC	1 cut(s)	1595
Sse9I	AATT	6 cut(s)	108, 153, 878, 912, 1274, 1568
SseBI	AGGCCT	3 cut(s)	869, 1153, 1217
SsiI	CCGC	9 cut(s)	16, 555, 829, 858, 909, 956, 1230, 1579, 1742
SspMI	CTAG	4 cut(s)	71, 378, 525, 1155
SstI	GAGCTC	2 cut(s)	26, 432
StuI	AGGCCT	3 cut(s)	869, 1153, 1217
StyD4I	CCNGG	4 cut(s)	326, 636, 843, 1635
StyI	CCWWGG	3 cut(s)	836, 1148, 1218
TaaI	ACNGT	5 cut(s)	278, 584, 1165, 1255, 1721
TaiI	ACGT	6 cut(s)	45, 347, 365, 562, 1479, 1680
TaqI	TCGA	5 cut(s)	314, 631, 760, 1631, 1706
TaqII	GACCGA	3 cut(s)	272, 883, 1392
TasI	AATT	6 cut(s)	108, 153, 878, 912, 1274, 1568
TatI	WGTACW	1 cut(s)	1449
TauI	GCSGC	1 cut(s)	831
TfiI	GAWTC	7 cut(s)	93, 370, 383, 611, 1545, 1663, 1781
Tru1I	TTAA	5 cut(s)	468, 816, 1257, 1316, 1526
Tru9I	TTAA	5 cut(s)	468, 816, 1257, 1316, 1526
TscAI	CASTG	5 cut(s)	613, 1110, 1168, 1416, 1659
TseFI	GTSAC	4 cut(s)	701, 970, 1165, 1551
TseI	GCWGC	2 cut(s)	831, 1466
Tsp45I	GTSAC	4 cut(s)	701, 970, 1165, 1551
TspDTI	ATGAA	6 cut(s)	17, 85, 422, 638, 1041, 1617
TspGWI	ACGGA	2 cut(s)	1049, 1087
TspRI	CASTG	5 cut(s)	613, 1110, 1168, 1416, 1659
Van91I	CCANNNNNTGG	1 cut(s)	1642
VpaK11BI	GGWCC	3 cut(s)	781, 895, 1375
XapI	RAATTY	1 cut(s)	1274
XceI	RCATGY	1 cut(s)	1595
XcmI	CCANNNNNNNNNTGG	4 cut(s)	125, 421, 1369, 1477
XmiI	GTMKAC	2 cut(s)	45, 1337
XspI	CTAG	4 cut(s)	71, 378, 525, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.