Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Forward (+)

8662093 .. 8669656

Gene Structure 7564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g14141.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGGTGAATATAACTTCCATCAAAACAGCATCTAATGGCCTGTGGCAAGGCGACAATCCTCTAAACTTCGCCTTCCCGCTCCTAATAGTTCAGACCACCTTGATCATCGTGGTTAGCCGCTTCCTCGCTTTCCTCCTCAAACCCCTCCGCCAACCCAAAGTCATTGCAGAAATTGTCGGTGGAGTTCTGCTTGGTCCTTCAGCTTTTGGACGAAACACAGAATACTTGCACACAATCTTTCCATCATGGAGCACCCCAATACTAGAATCAGTAGCAAGTATTGGTCTACTATTTTACTTGTTCCTTGTAGGCTTAGAGCTCGACCTGAGCTCCACTCGCCGAAGTGGCACTAAGGCTTTCGGAATAGCCATCGCCGGCATAACGCTCCCTTTCCTCTGTGGCATCGGCGTCGCTTTGGTCTTCCGCAAAACAATTTACGGAGCTGACAAAGCAGGGTTCACTCAGTTCCTTGTCTTCATGGGAGTGGCACTCTCCATCACCGCCTTCCCTGTGCTTGCCCGCATCTTAGCGGAGCTCAAACTCTTAACGACTCAGATCGGGGAGACCGCCATGGCCGCTGCTGCCTTCAATGACGTTGGTGCATGGATCTTGTTAGCCTTGGCGGTGGCCTTGGCAGGTGACGGTGCTGGTGGCCACAACAAGAGCCCCTTGATATCCATTTGGGTCCTCCTTTCAGGTGTTGCATTTGTCGCGTTCATGATGGTCGTGATCCGACCGGCCATGAAATGGGTCGCCAGTCGGTGCACACCGGAGCAAGACGTCGTGGACGAAGCCTATATTTGCTTAACCTTAGCCGGAGTAATGGTGGCCGGGTTCATTACTGACCTAATTGGAATTCATTCCATATTTGGAGCTTTCATCTTCGGACTCACAATTCCGAAAGGACAGTTTGCAGATAGATTAATATCGAGGATTGAAGACTTTGTGTCCGGCTTGCTGCTTCCTCTTTACTTTGCGTCGAGTGGGTTGAAGACGGATGTGGCTAAAATACGTGGCGGCGGGGCATGGGGGTTGTTGGCTCTGGTTATCACCACGGCGTGCGCCGGGAAGATTCTGGGGACGTTTGTGGTGGCGATGATGTTTATGATTCCGGTAAGAGAGTCCTTGACGCTTGGTGTGCTCATGAACACCAAAGGCTTGGTGGAACTCATCGTCCTAAACATTGGCAAGGAGAAGAAGGTACTAAATGATGAGTCATTTGCGATTCTAGTCCTCATGGCACTCTTCACCACCTTCATCACAAGTCCGATCGCGATGGCCATTTACAAACCGGCACGTGGAATCTCATTTTCCACTCACCGTAAACTATGTGATCTCTCCACAGTTGATCAAGCTTCCAAAGACGAGCTCCGGATACTTGCATGTGTTCATGGTCCAAACAATGCACCTTCACTCATAAGCTTCATAGATTCCATTCGAAGCACCAAAAATTCCCAACTCAAACTCTTCCTAATGCACCTTGTTGAACTCACCGAGCGCTCTTCTTCAATCGTTATGGTCCAACGGGCTCGAAAAAATGGGTATCCCTTCTTTAATCGAAGGCCTCGAGGTGAGTTGTATGATAGGGTTTATGGGGCATTCCAAGCCTATAGTCAATTGGGCCGAGTCTCGGTCAGGCCCACAACCGTAATTTCACCCTTGTCCACAATGTACGAGGATATTTGCCATGTGGCGGAAGATAAGAGGGCGACGATGATCGTCTTACCATTCCACAAGCAATGGAGATGTGATGGTGAAGATCATGAGAAAAAAGAGATCAATTTTGGGAATGCTTGGAGAGGGGTCAACCAAAGGGTGCTTCAAAATGCGCCGTGCTCGGTTGCGGTGCTTGTGGATCGTGGATTCGGAAATTTTGAAGCCCAAACTCCCGAGCCGGATAAAGTTGTGGCCCGACTAATCTGTATTCTATTTTTCGGTGGGCCAGATGATCGAGAGGCCTTGGAATTGGGTGGTCGGATGGCTGACCACCCATTGGTTAAAGTGAAGGTTGTGAGATTCGTCGAGAAAGAAGGTTTGGAGAGTAATGGGCCTCATGGGCCTATGTTTTGCCCCCTACCCACCAAGTCCACCGAGAACAGTTATAGCTTCTCCACAAACAAAATGGACCGAGGGAAAGAAAAGGAACTAGATGAGGCTGCATTGGCTGAGTTCAGGAGCAAACTAGTGGAGGATGGGAAAGCTGAGTACACTGAGCAAGTGGTGGCTAGGAATATTGTGGAGGGGGTGCTGGCAATAGTACGAGGTGGAGATCAGGACCTAATAATTGTAGGAAGAGGACGGTTCTCATCAAGCATGGTGGTGGAATTGCCAGACCGGCAAGCCGAGCACTCCGAGCTGGGGCCTGTTGGTGACATATTGGCCTCTTCCGGCCATGGTGTTGTCTCATCAGTCCTTGTGATTCAACAGCACGATGTAGCTCATGCTAGAGGAAGCTTCGGTGTCCAAGATAGTGCATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

827

Amino Acids

89.82

Weight (kDa)

8.35

Isoelectric Point (pI)

32.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	40 - 424	3.1e-57	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	486 - 624	5.8e-37	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	641 - 673	9.9e-06	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	626
AatII	GACGTC	1 cut(s)	783
AbsI	CCTCGAGG	1 cut(s)	1566
Acc36I	ACCTGC	1 cut(s)	626
AccB7I	CCANNNNNTGG	2 cut(s)	747, 1993
AccBSI	CCGCTC	1 cut(s)	79
AccI	GTMKAC	1 cut(s)	286
AccII	CGCG	2 cut(s)	713, 1274
AccIII	TCCGGA	1 cut(s)	1371
AclWI	GGATC	3 cut(s)	614, 724, 1863
AcoI	YGGCCR	6 cut(s)	573, 652, 738, 828, 1278, 2389
AcsI	RAATTY	3 cut(s)	855, 1450, 1870
AcuI	CTGAAG	1 cut(s)	183
AcvI	CACGTG	1 cut(s)	1298
AcyI	GRCGYC	2 cut(s)	408, 780
AdeI	CACNNNGTG	1 cut(s)	1059
AfaI	GTAC	4 cut(s)	1203, 1673, 2207, 2259
AfeI	AGCGCT	1 cut(s)	1499
AfiI	CCNNNNNNNGG	6 cut(s)	747, 1298, 1630, 1693, 1993, 2358
AgsI	TTSAA	8 cut(s)	589, 938, 991, 1487, 1509, 1823, 1877, 2423
AhdI	GACNNNNNGTC	1 cut(s)	946
AhlI	ACTAGT	1 cut(s)	2182
AjuI	GAANNNNNNNTTGG	4 cut(s)	1803, 1835, 2018, 2050
AloI	GAACNNNNNNTCC	2 cut(s)	1158, 1190
Alw21I	GWGCWC	9 cut(s)	254, 321, 332, 537, 767, 1143, 1371, 1838, 2349
Alw26I	GTCTC	3 cut(s)	557, 1633, 2407
Alw44I	GTGCAC	1 cut(s)	763
AlwI	GGATC	3 cut(s)	614, 724, 1863
Ama87I	CYCGRG	2 cut(s)	1566, 1889
Aor13HI	TCCGGA	1 cut(s)	1371
Aor51HI	AGCGCT	1 cut(s)	1499
ApaLI	GTGCAC	1 cut(s)	763
ApeKI	GCWGC	4 cut(s)	578, 581, 958, 2156
ApoI	RAATTY	3 cut(s)	855, 1450, 1870
AseI	ATTAAT	1 cut(s)	923
Asp700I	GAANNNNTTC	1 cut(s)	859
AspLEI	GCGC	3 cut(s)	1064, 1500, 1831
AsuC2I	CCSGG	2 cut(s)	832, 1066
AsuII	TTCGAA	1 cut(s)	1438
AvaI	CYCGRG	2 cut(s)	1566, 1889
AvaII	GGWCC	6 cut(s)	194, 685, 1394, 1519, 2125, 2275
BaeGI	GKGCMC	1 cut(s)	767
BalI	TGGCCA	2 cut(s)	654, 1280
BanII	GRGCYC	6 cut(s)	321, 332, 537, 668, 1371, 1531
BbrPI	CACGTG	1 cut(s)	1298
BbsI	GAAGAC	4 cut(s)	412, 466, 945, 998
Bbv12I	GWGCWC	9 cut(s)	254, 321, 332, 537, 767, 1143, 1371, 1838, 2349
BbvI	GCAGC	4 cut(s)	565, 568, 945, 2143
BccI	CCATC	9 cut(s)	26, 250, 377, 503, 715, 1270, 1745, 1972, 2186
BceAI	ACGGC	2 cut(s)	1071, 1816
BcgI	CGANNNNNNTGC	2 cut(s)	391, 425
BciVI	GTATCC	2 cut(s)	1368, 1554
BclI	TGATCA	2 cut(s)	102, 1348
BcnI	CCSGG	2 cut(s)	832, 1066
BcoDI	GTCTC	3 cut(s)	557, 1633, 2407
BcuI	ACTAGT	1 cut(s)	2182
BfaI	CTAG	6 cut(s)	263, 1229, 2147, 2183, 2226, 2445
BfmI	CTRYAG	1 cut(s)	1609
BfoI	RGCGCY	1 cut(s)	1501
BfuAI	ACCTGC	1 cut(s)	626
BfuI	GTATCC	2 cut(s)	1368, 1554
BglI	GCCNNNNNGGC	3 cut(s)	345, 2056, 2335
BisI	GCNGC	7 cut(s)	118, 576, 579, 582, 959, 1018, 2157
BlsI	GCNGC	7 cut(s)	119, 577, 580, 583, 960, 1019, 2158
Bme1390I	CCNGG	2 cut(s)	832, 1066
Bme18I	GGWCC	6 cut(s)	194, 685, 1394, 1519, 2125, 2275
BmeRI	GACNNNNNGTC	1 cut(s)	946
BmeT110I	CYCGRG	2 cut(s)	1566, 1889
BmiI	GGNNCC	2 cut(s)	686, 2361
BmrFI	CCNGG	2 cut(s)	832, 1066
BmsI	GCATC	3 cut(s)	38, 411, 531
BpiI	GAAGAC	4 cut(s)	412, 466, 945, 998
BplI	GAGNNNNNCTC	4 cut(s)	319, 351, 474, 506
Bpu10I	CCTNAGC	2 cut(s)	326, 811
Bpu14I	TTCGAA	1 cut(s)	1438
BpuMI	CCSGG	2 cut(s)	832, 1066
BsaAI	YACGTR	2 cut(s)	1013, 1298
BsaBI	GATNNNNATC	1 cut(s)	925
BsaHI	GRCGYC	2 cut(s)	408, 780
BsaI	GGTCTC	1 cut(s)	557
BsaJI	CCNNGG	7 cut(s)	570, 618, 630, 1053, 1959, 2128, 2392
BsaWI	WCCGGW	3 cut(s)	769, 1111, 1371
BsaXI	ACNNNNNCTCC	4 cut(s)	314, 344, 810, 840
Bsc4I	CCNNNNNNNGG	6 cut(s)	747, 1298, 1630, 1693, 1993, 2358
Bse118I	RCCGGY	4 cut(s)	374, 736, 1291, 2334
Bse1I	ACTGG	1 cut(s)	756
Bse3DI	GCAATG	2 cut(s)	162, 1745
Bse8I	GATNNNNATC	1 cut(s)	925
BseAI	TCCGGA	1 cut(s)	1371
BseDI	CCNNGG	7 cut(s)	570, 618, 630, 1053, 1959, 2128, 2392
BseGI	GGATG	3 cut(s)	1003, 1983, 2197
BseJI	GATNNNNATC	1 cut(s)	925
BseLI	CCNNNNNNNGG	6 cut(s)	747, 1298, 1630, 1693, 1993, 2358
BseMI	GCAATG	2 cut(s)	162, 1745
BseMII	CTCAG	6 cut(s)	317, 476, 566, 2157, 2193, 2202
BseNI	ACTGG	1 cut(s)	756
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	1 cut(s)	767
BseXI	GCAGC	4 cut(s)	565, 568, 945, 2143
BseYI	CCCAGC	1 cut(s)	2356
Bsh1236I	CGCG	2 cut(s)	713, 1274
Bsh1285I	CGRYCG	2 cut(s)	737, 1272
BsiEI	CGRYCG	2 cut(s)	737, 1272
BsiHKAI	GWGCWC	9 cut(s)	254, 321, 332, 537, 767, 1143, 1371, 1838, 2349
BsiHKCI	CYCGRG	2 cut(s)	1566, 1889
BslFI	GGGAC	1 cut(s)	1093
BslI	CCNNNNNNNGG	6 cut(s)	747, 1298, 1630, 1693, 1993, 2358
BsmAI	GTCTC	3 cut(s)	557, 1633, 2407
BsmFI	GGGAC	1 cut(s)	1093
BsmI	GAATGC	2 cut(s)	1598, 1795
Bso31I	GGTCTC	1 cut(s)	557
BsoBI	CYCGRG	2 cut(s)	1566, 1889
Bsp119I	TTCGAA	1 cut(s)	1438
Bsp13I	TCCGGA	1 cut(s)	1371
Bsp19I	CCATGG	2 cut(s)	570, 2392
Bsp68I	TCGCGA	1 cut(s)	1274
BspCNI	CTCAG	6 cut(s)	318, 475, 565, 2158, 2194, 2203
BspEI	TCCGGA	1 cut(s)	1371
BspFNI	CGCG	2 cut(s)	713, 1274
BspHI	TCATGA	3 cut(s)	717, 1143, 1762
BspLI	GGNNCC	2 cut(s)	686, 2361
BspMI	ACCTGC	1 cut(s)	626
BspPI	GGATC	3 cut(s)	614, 724, 1863
BspQI	GCTCTTC	1 cut(s)	1507
BspT104I	TTCGAA	1 cut(s)	1438
BspTNI	GGTCTC	1 cut(s)	557
BsrBI	CCGCTC	1 cut(s)	79
BsrDI	GCAATG	2 cut(s)	162, 1745
BsrFI	RCCGGY	4 cut(s)	374, 736, 1291, 2334
BsrI	ACTGG	1 cut(s)	756
BssAI	RCCGGY	4 cut(s)	374, 736, 1291, 2334
BssECI	CCNNGG	7 cut(s)	570, 618, 630, 1053, 1959, 2128, 2392
BssNI	GRCGYC	2 cut(s)	408, 780
BssT1I	CCWWGG	5 cut(s)	570, 618, 630, 1959, 2392
Bst4CI	ACNGT	7 cut(s)	644, 909, 1322, 1345, 1648, 2099, 2301
Bst6I	CTCTTC	5 cut(s)	1250, 1472, 1507, 2287, 2389
BstACI	GRCGYC	2 cut(s)	408, 780
BstBAI	YACGTR	2 cut(s)	1013, 1298
BstBI	TTCGAA	1 cut(s)	1438
BstC8I	GCNNGC	7 cut(s)	376, 516, 520, 956, 1060, 2250, 2340
BstDSI	CCRYGG	3 cut(s)	570, 1053, 2392
BstF5I	GGATG	3 cut(s)	1003, 1983, 2197
BstFNI	CGCG	2 cut(s)	713, 1274
BstH2I	RGCGCY	1 cut(s)	1501
BstHHI	GCGC	3 cut(s)	1064, 1500, 1831
BstMAI	GTCTC	3 cut(s)	557, 1633, 2407
BstMCI	CGRYCG	2 cut(s)	737, 1272
BstNSI	RCATGY	1 cut(s)	1386
BstSCI	CCNGG	2 cut(s)	830, 1064
BstSFI	CTRYAG	1 cut(s)	1609
BstSLI	GKGCMC	1 cut(s)	767
BstUI	CGCG	2 cut(s)	713, 1274
BstV1I	GCAGC	4 cut(s)	565, 568, 945, 2143
BstV2I	GAAGAC	4 cut(s)	412, 466, 945, 998
BstX2I	RGATCY	1 cut(s)	606
BstXI	CCANNNNNNTGG	1 cut(s)	1159
BstYI	RGATCY	1 cut(s)	606
BsuI	GTATCC	2 cut(s)	1368, 1554
BtgI	CCRYGG	3 cut(s)	570, 1053, 2392
BtgZI	GCGATG	3 cut(s)	355, 1109, 1289
BtsCI	GGATG	3 cut(s)	1003, 1983, 2197
BtsIMutI	CAGTG	1 cut(s)	2208
BtuMI	TCGCGA	1 cut(s)	1274
BveI	ACCTGC	1 cut(s)	626
Cac8I	GCNNGC	7 cut(s)	376, 516, 520, 956, 1060, 2250, 2340
CciI	TCATGA	3 cut(s)	717, 1143, 1762
CfoI	GCGC	3 cut(s)	1064, 1500, 1831
Cfr10I	RCCGGY	4 cut(s)	374, 736, 1291, 2334
CseI	GACGC	3 cut(s)	397, 966, 1138
Csp6I	GTAC	4 cut(s)	1202, 1672, 2206, 2258
CspCI	CAANNNNNGTGG	2 cut(s)	2298, 2333
CviQI	GTAC	4 cut(s)	1202, 1672, 2206, 2258
DraIII	CACNNNGTG	1 cut(s)	1059
DriI	GACNNNNNGTC	1 cut(s)	946
EaeI	YGGCCR	6 cut(s)	573, 652, 738, 828, 1278, 2389
Eam1104I	CTCTTC	5 cut(s)	1250, 1472, 1507, 2287, 2389
Eam1105I	GACNNNNNGTC	1 cut(s)	946
EarI	CTCTTC	5 cut(s)	1250, 1472, 1507, 2287, 2389
EciI	GGCGGA	2 cut(s)	137, 1709
Ecl136II	GAGCTC	4 cut(s)	319, 330, 535, 1369
Eco130I	CCWWGG	5 cut(s)	570, 618, 630, 1959, 2392
Eco147I	AGGCCT	2 cut(s)	1564, 1958
Eco24I	GRGCYC	6 cut(s)	321, 332, 537, 668, 1371, 1531
Eco31I	GGTCTC	1 cut(s)	557
Eco32I	GATATC	1 cut(s)	675
Eco47I	GGWCC	6 cut(s)	194, 685, 1394, 1519, 2125, 2275
Eco47III	AGCGCT	1 cut(s)	1499
Eco53kI	GAGCTC	4 cut(s)	319, 330, 535, 1369
Eco57I	CTGAAG	1 cut(s)	183
Eco72I	CACGTG	1 cut(s)	1298
Eco88I	CYCGRG	2 cut(s)	1566, 1889
EcoICRI	GAGCTC	4 cut(s)	319, 330, 535, 1369
EcoO109I	RGGNCCY	3 cut(s)	685, 2275, 2360
EcoRI	GAATTC	1 cut(s)	855
EcoRV	GATATC	1 cut(s)	675
EcoT14I	CCWWGG	5 cut(s)	570, 618, 630, 1959, 2392
EcoT38I	GRGCYC	6 cut(s)	321, 332, 537, 668, 1371, 1531
ErhI	CCWWGG	5 cut(s)	570, 618, 630, 1959, 2392
FalI	AAGNNNNNCTT	2 cut(s)	653, 685
FaqI	GGGAC	1 cut(s)	1093
FauI	CCCGC	3 cut(s)	84, 527, 1013
FbaI	TGATCA	2 cut(s)	102, 1348
FblI	GTMKAC	1 cut(s)	286
Fnu4HI	GCNGC	7 cut(s)	118, 576, 579, 582, 959, 1018, 2157
FokI	GGATG	3 cut(s)	1010, 1990, 2204
FriOI	GRGCYC	6 cut(s)	321, 332, 537, 668, 1371, 1531
Fsp4HI	GCNGC	7 cut(s)	118, 576, 579, 582, 959, 1018, 2157
FspBI	CTAG	6 cut(s)	263, 1229, 2147, 2183, 2226, 2445
GlaI	GCGC	3 cut(s)	1063, 1499, 1830
GluI	GCNGC	7 cut(s)	118, 576, 579, 582, 959, 1018, 2157
GsaI	CCCAGC	1 cut(s)	2360
HaeII	RGCGCY	1 cut(s)	1501
HgaI	GACGC	3 cut(s)	397, 966, 1138
HhaI	GCGC	3 cut(s)	1064, 1500, 1831
Hin1I	GRCGYC	2 cut(s)	408, 780
Hin6I	GCGC	3 cut(s)	1062, 1498, 1829
HinP1I	GCGC	3 cut(s)	1062, 1498, 1829
HincII	GTYRAC	1 cut(s)	1807
HindII	GTYRAC	1 cut(s)	1807
HindIII	AAGCTT	3 cut(s)	1353, 1420, 2452
Hpy166II	GTNNAC	9 cut(s)	287, 459, 765, 787, 1325, 1665, 1807, 2088, 2208
Hpy8I	GTNNAC	9 cut(s)	287, 459, 765, 787, 1325, 1665, 1807, 2088, 2208
Hpy99I	CGWCG	5 cut(s)	413, 785, 982, 1714, 2024
HpyCH4III	ACNGT	7 cut(s)	644, 909, 1322, 1345, 1648, 2099, 2301
HpyCH4IV	ACGT	5 cut(s)	594, 780, 1012, 1082, 1297
HpySE526I	ACGT	5 cut(s)	594, 780, 1012, 1082, 1297
Hsp92I	GRCGYC	2 cut(s)	408, 780
HspAI	GCGC	3 cut(s)	1062, 1498, 1829
Kpn2I	TCCGGA	1 cut(s)	1371
KroI	GCCGGC	1 cut(s)	374
KroNI	GCCGGC	1 cut(s)	376
Ksp22I	TGATCA	2 cut(s)	102, 1348
LguI	GCTCTTC	1 cut(s)	1507
Lsp1109I	GCAGC	4 cut(s)	565, 568, 945, 2143
LweI	GCATC	3 cut(s)	38, 411, 531
MaeI	CTAG	6 cut(s)	263, 1229, 2147, 2183, 2226, 2445
MaeII	ACGT	5 cut(s)	594, 780, 1012, 1082, 1297
MaeIII	GTNAC	2 cut(s)	638, 2369
MbiI	CCGCTC	1 cut(s)	79
MfeI	CAATTG	1 cut(s)	1616
MflI	RGATCY	1 cut(s)	606
MlsI	TGGCCA	2 cut(s)	654, 1280
MluNI	TGGCCA	2 cut(s)	654, 1280
MlyI	GAGTC	5 cut(s)	544, 882, 1130, 1223, 1635
MmeI	TCCRAC	3 cut(s)	757, 1546, 1955
Mox20I	TGGCCA	2 cut(s)	654, 1280
MroI	TCCGGA	1 cut(s)	1371
MroNI	GCCGGC	1 cut(s)	374
MroXI	GAANNNNTTC	1 cut(s)	859
MscI	TGGCCA	2 cut(s)	654, 1280
MseI	TTAA	5 cut(s)	545, 806, 923, 1554, 1998
MslI	CAYNNNNRTG	2 cut(s)	482, 2318
Msp20I	TGGCCA	2 cut(s)	654, 1280
MspA1I	CMGCKG	1 cut(s)	578
MspR9I	CCNGG	2 cut(s)	832, 1066
MunI	CAATTG	1 cut(s)	1616
Mva1269I	GAATGC	2 cut(s)	1598, 1795
MvnI	CGCG	2 cut(s)	713, 1274
NaeI	GCCGGC	1 cut(s)	376
NciI	CCSGG	2 cut(s)	832, 1066
NcoI	CCATGG	2 cut(s)	570, 2392
NgoMIV	GCCGGC	1 cut(s)	374
NlaIV	GGNNCC	2 cut(s)	686, 2361
NmeAIII	GCCGAG	2 cut(s)	1649, 2368
NmuCI	GTSAC	2 cut(s)	638, 2369
NruI	TCGCGA	1 cut(s)	1274
NspI	RCATGY	1 cut(s)	1386
NspV	TTCGAA	1 cut(s)	1438
PaeR7I	CTCGAG	1 cut(s)	1566
PagI	TCATGA	3 cut(s)	717, 1143, 1762
PaqCI	CACCTGC	1 cut(s)	626
PceI	AGGCCT	2 cut(s)	1564, 1958
PciSI	GCTCTTC	1 cut(s)	1507
PcsI	WCGNNNNNNNCGW	2 cut(s)	786, 1564
PctI	GAATGC	2 cut(s)	1598, 1795
PdiI	GCCGGC	1 cut(s)	376
PdmI	GAANNNNTTC	1 cut(s)	859
PfeI	GAWTC	9 cut(s)	266, 1072, 1108, 1225, 1302, 1430, 1863, 2016, 2419
PflMI	CCANNNNNTGG	2 cut(s)	747, 1993
PkrI	GCNGC	7 cut(s)	119, 577, 580, 583, 960, 1019, 2158
Ple19I	CGATCG	1 cut(s)	1272
PleI	GAGTC	5 cut(s)	544, 882, 1129, 1222, 1634
PmaCI	CACGTG	1 cut(s)	1298
PmlI	CACGTG	1 cut(s)	1298
PpsI	GAGTC	5 cut(s)	544, 882, 1129, 1222, 1634
Ppu21I	YACGTR	2 cut(s)	1013, 1298
PpuMI	RGGWCCY	2 cut(s)	685, 2275
PshBI	ATTAAT	1 cut(s)	923
Psp124BI	GAGCTC	4 cut(s)	321, 332, 537, 1371
Psp5II	RGGWCCY	2 cut(s)	685, 2275
PspCI	CACGTG	1 cut(s)	1298
PspFI	CCCAGC	1 cut(s)	2356
PspN4I	GGNNCC	2 cut(s)	686, 2361
PspPPI	RGGWCCY	2 cut(s)	685, 2275
PspXI	VCTCGAGB	1 cut(s)	1566
PsuI	RGATCY	1 cut(s)	606
PvuI	CGATCG	1 cut(s)	1272
RruI	TCGCGA	1 cut(s)	1274
RsaI	GTAC	4 cut(s)	1203, 1673, 2207, 2259
RsaNI	GTAC	4 cut(s)	1202, 1672, 2206, 2258
RseI	CAYNNNNRTG	2 cut(s)	482, 2318
SacI	GAGCTC	4 cut(s)	321, 332, 537, 1371
SapI	GCTCTTC	1 cut(s)	1507
SaqAI	TTAA	5 cut(s)	545, 806, 923, 1554, 1998
SatI	GCNGC	7 cut(s)	118, 576, 579, 582, 959, 1018, 2157
SchI	GAGTC	5 cut(s)	544, 882, 1130, 1223, 1635
ScrFI	CCNGG	2 cut(s)	832, 1066
SfaNI	GCATC	3 cut(s)	38, 411, 531
SfcI	CTRYAG	1 cut(s)	1609
SfiI	GGCCNNNNNGGCC	1 cut(s)	2056
Sfr274I	CTCGAG	1 cut(s)	1566
SfuI	TTCGAA	1 cut(s)	1438
SinI	GGWCC	6 cut(s)	194, 685, 1394, 1519, 2125, 2275
SlaI	CTCGAG	1 cut(s)	1566
SmiMI	CAYNNNNRTG	2 cut(s)	482, 2318
SmlI	CTYRAG	1 cut(s)	1566
SmoI	CTYRAG	1 cut(s)	1566
SpeI	ACTAGT	1 cut(s)	2182
SseBI	AGGCCT	2 cut(s)	1564, 1958
SspI	AATATT	1 cut(s)	2233
SspMI	CTAG	6 cut(s)	263, 1229, 2147, 2183, 2226, 2445
SstI	GAGCTC	4 cut(s)	321, 332, 537, 1371
StuI	AGGCCT	2 cut(s)	1564, 1958
StyD4I	CCNGG	2 cut(s)	830, 1064
StyI	CCWWGG	5 cut(s)	570, 618, 630, 1959, 2392
TaaI	ACNGT	7 cut(s)	644, 909, 1322, 1345, 1648, 2099, 2301
TaiI	ACGT	5 cut(s)	597, 783, 1015, 1085, 1300
TaqI	TCGA	9 cut(s)	321, 929, 980, 1438, 1531, 1558, 1567, 1951, 2022
TaqII	GACCGA	2 cut(s)	1621, 2142
TatI	WGTACW	1 cut(s)	2205
TauI	GCSGC	3 cut(s)	120, 578, 1020
TfiI	GAWTC	9 cut(s)	266, 1072, 1108, 1225, 1302, 1430, 1863, 2016, 2419
Tru1I	TTAA	5 cut(s)	545, 806, 923, 1554, 1998
Tru9I	TTAA	5 cut(s)	545, 806, 923, 1554, 1998
TscAI	CASTG	1 cut(s)	2215
TseFI	GTSAC	2 cut(s)	638, 2369
TseI	GCWGC	4 cut(s)	578, 581, 958, 2156
Tsp45I	GTSAC	2 cut(s)	638, 2369
TspGWI	ACGGA	2 cut(s)	453, 1010
TspRI	CASTG	1 cut(s)	2215
Van91I	CCANNNNNTGG	2 cut(s)	747, 1993
VneI	GTGCAC	1 cut(s)	763
VpaK11BI	GGWCC	6 cut(s)	194, 685, 1394, 1519, 2125, 2275
VspI	ATTAAT	1 cut(s)	923
XapI	RAATTY	3 cut(s)	855, 1450, 1870
XceI	RCATGY	1 cut(s)	1386
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1159, 2119
XhoI	CTCGAG	1 cut(s)	1566
XmiI	GTMKAC	1 cut(s)	286
XmnI	GAANNNNTTC	1 cut(s)	859
XspI	CTAG	6 cut(s)	263, 1229, 2147, 2183, 2226, 2445
ZraI	GACGTC	1 cut(s)	781

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.