Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Forward (+)

12369287 .. 12374359

Gene Structure 5073 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G012600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2070 bp

ATGGTGAATATAACCACCATCAGAACAGCATCAAATGGCCTGTGGCAAGGCGACAACCCTTTAAACTTCGCCTTCCCACTCCTAATAGTTCAGACCACCTTGATCATCGTCGTCAGCCGCTTCCTTGCTTTCCTACTGAAACCCCTCCACCAACCCAAAGTCATTGCAGAGATTGTCGGTGGAATTCTGCTTGGTCCTTCAGCTTTCGGACGAAACATAGATTACTTGCACAAAATCTTTCCTTCACGGGGCACTCCAATATTAGAATCAGTGGCAAGTATTGGTCTTCTATTTTACTTGTTCCTTGTAGGCTTAGAGCTCGACCTGAGCTCCACTCGCCGAAGTGGCAGAAGGGGTTTCAGCATAGCCCTCGCCGGCATAACGCTCCCTTTCTTCTTCGGCATCGGCGTCGCTCTGGTCTTCCGTAAAACAATTGTTGGAGCTGACAAAGCAGGGTTCACTCAGTTCCTTGTCTTCATGGGAGTTGCCCTCTCCATCACCGCCTTCCCTGTGCTTGCCCGCATCTTAGCGGAGCTCAAACTGTTAACAACTCAGATTGGGGAGACTGCCATGGCGGCTGCTGCCGTCAACGACGTTGGTGCATGGATCTTGCTGGCCGTGGCCGTGGCCCTGGCAGGTGACGGTGCTGGTGGCCACAACAAGAGCCCTTTGATATGCATTTGGGTCCTCCTATCAGGTGTTGCCTTTGTGGCCTTCATGATGGTCGTGATCCGACCTGCCATGAAATGGGTCGCCAGCCGGTGCACACCGGAGCAAGATGTCTTGGACGAAGCCTATATTTGCTTAACCTTAACTGGAGTAATGGTGGCCGGGTTCATGACTGACATAATTGGAATTCATTCCATATTTGGAGCTTTCGTCTTCGGACTCACAATTCCGAAAGGACAGTTTGCAGATAGATTAATATCGAGGATTGAAGACTTTGTGTCCGGCTTGTTGCTTCCGCTTTACTTTGCATCAAGTGGGTTGAAAACAGATGTGGCTAAAATACGTGGCGGCAGGGCATGGGGGTTGTTGGCTCTGGTTATAACCACGGCTTGCGCTGGGAAGATTCTCGGGACGTTTGTGGTGGCAATGATGTTTATGATTCCTGCCAGAGAGTCATTGACGCTTGGTGTGCTCATGAACACTAAAGGCTTGGTGGAGCTCATTGTTCTAAACATTGGCAAGGAGCAGAAGGTGCTAAATGACGAGTCATTTGCAATTCTAGTCCTCATGGCACTCTCCACCACCTTCATTACATGTCCGGTAGTGATGGCCATTTACAGACCGGCACGTGGAATCTCTATTCGCACTCACCGTAAACTTTGTGATCTCTCCACAGCTGATCAAGCCGCCAAAGACGAGCTCCGAATTCTTGCATGTGTTCATGGTCCAAACAATGCACCCTCGCTCATAAGCTTAATAGATTCAATTCGAAGCACCAAAAAATCCCAACTCAAACTCTTCCTAATGCACCTTGTTGAACTCACGGAGCGCTCTTCTTCAATCGTTATGGTCCAACGGGCTCGAAAAAATGGGTTTCCTTTCTTTAATCGAACGGCTCGCGGTGAGTGGTATGATAGGGTAGCTGGGGCATTCCAAGCCTATAGTCAATTGGGCCAAGTCACGGTCCGGCCCACAACTGCAATTTCAGCTTTGTCCACAATGTATGAGGATATTTGCCATGCGGCGGAAGAGAAGAGGGCGGCGATGATCGTCTTGCCATTCCACAAGCAATGGAGATGTGATGGTGATGATCATGAGAAAAAAGAGATTGATGTGGGCAATGATTGGAGAGGGGTCAACCAAAGAGTGCTCCAAAATGCGCCGTGCTCGGTTGCAGTGCTTGTGGACCGTGGATTTGATAATTTCAAAGCCCAAAGTCTCGAGCCGGATAAGGTTGTGGCCCTAAGGATTTGTATTGTGTTTTTGGGTGGGCCTGATGATCGAGAGGCCTTGGAATTGGGTGGTCGGATGGCTGACCACCCGGCGGTTAAAGTGAAGGTTGTGAGGTTCGTCGAGAAGAAGGTCTGGAGAGTAGTAATGGGTCTCATGGGCTTATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

689

Amino Acids

75.07

Weight (kDa)

9.32

Isoelectric Point (pI)

32.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	40 - 421	2.6e-54	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	486 - 623	4.3e-38	Plant cation/H(+) antiporter domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1049
AarI	CACCTGC	1 cut(s)	626
Acc36I	ACCTGC	2 cut(s)	626, 745
AccB7I	CCANNNNNTGG	1 cut(s)	747
AccII	CGCG	1 cut(s)	1569
AclWI	GGATC	2 cut(s)	614, 724
AcoI	YGGCCR	5 cut(s)	615, 621, 652, 828, 1278
AcsI	RAATTY	3 cut(s)	183, 855, 1374
AcuI	CTGAAG	1 cut(s)	183
AcvI	CACGTG	1 cut(s)	1298
AcyI	GRCGYC	1 cut(s)	408
AfeI	AGCGCT	1 cut(s)	1499
AfiI	CCNNNNNNNGG	6 cut(s)	248, 747, 1298, 1630, 1693, 1993
AflIII	ACRYGT	1 cut(s)	1262
AgsI	TTSAA	6 cut(s)	938, 991, 1434, 1487, 1509, 1876
AhdI	GACNNNNNGTC	1 cut(s)	946
AjnI	CCWGG	1 cut(s)	630
Alw21I	GWGCWC	9 cut(s)	321, 332, 537, 767, 1143, 1170, 1371, 1821, 1838
Alw26I	GTCTC	3 cut(s)	557, 1892, 2057
Alw44I	GTGCAC	1 cut(s)	763
AlwI	GGATC	2 cut(s)	614, 724
Ama87I	CYCGRG	2 cut(s)	1076, 1889
Aor51HI	AGCGCT	1 cut(s)	1499
ApaLI	GTGCAC	1 cut(s)	763
ApeKI	GCWGC	2 cut(s)	578, 581
ApoI	RAATTY	3 cut(s)	183, 855, 1374
ArsI	GACNNNNNNTTYG	4 cut(s)	1202, 1234, 1847, 1879
AseI	ATTAAT	1 cut(s)	923
Asp700I	GAANNNNTTC	1 cut(s)	859
AspLEI	GCGC	3 cut(s)	1064, 1500, 1831
AsuC2I	CCSGG	2 cut(s)	832, 1991
AsuHPI	GGTGA	6 cut(s)	16, 490, 650, 1310, 1583, 1766
AsuII	TTCGAA	1 cut(s)	1438
AvaI	CYCGRG	2 cut(s)	1076, 1889
AvaII	GGWCC	6 cut(s)	194, 685, 1394, 1519, 1633, 1855
AxyI	CCTNAGG	1 cut(s)	1913
BaeGI	GKGCMC	2 cut(s)	254, 767
BalI	TGGCCA	2 cut(s)	654, 1280
BanII	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
BbrPI	CACGTG	1 cut(s)	1298
BbsI	GAAGAC	5 cut(s)	278, 412, 466, 874, 945
Bbv12I	GWGCWC	9 cut(s)	321, 332, 537, 767, 1143, 1170, 1371, 1821, 1838
BbvI	GCAGC	2 cut(s)	565, 568
BccI	CCATC	6 cut(s)	26, 503, 715, 1270, 1745, 1972
BceAI	ACGGC	6 cut(s)	569, 602, 608, 1071, 1578, 1816
BcgI	CGANNNNNNTGC	8 cut(s)	352, 386, 391, 425, 581, 615, 1202, 1236
BciT130I	CCWGG	1 cut(s)	632
BclI	TGATCA	3 cut(s)	102, 1348, 1759
BcnI	CCSGG	2 cut(s)	832, 1991
BcoDI	GTCTC	3 cut(s)	557, 1892, 2057
BfaI	CTAG	1 cut(s)	1229
BfmI	CTRYAG	1 cut(s)	1609
BfoI	RGCGCY	1 cut(s)	1501
BfuAI	ACCTGC	2 cut(s)	626, 745
BglI	GCCNNNNNGGC	3 cut(s)	345, 575, 710
BisI	GCNGC	8 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710
BlsI	GCNGC	8 cut(s)	119, 577, 580, 583, 1019, 1357, 1693, 1711
Bme1390I	CCNGG	3 cut(s)	632, 832, 1991
Bme18I	GGWCC	6 cut(s)	194, 685, 1394, 1519, 1633, 1855
BmeRI	GACNNNNNGTC	1 cut(s)	946
BmeT110I	CYCGRG	2 cut(s)	1076, 1889
BmiI	GGNNCC	1 cut(s)	686
BmrFI	CCNGG	3 cut(s)	632, 832, 1991
BmsI	GCATC	4 cut(s)	38, 411, 531, 986
BpiI	GAAGAC	5 cut(s)	278, 412, 466, 874, 945
BplI	GAGNNNNNCTC	4 cut(s)	319, 351, 474, 506
BpmI	CTGGAG	2 cut(s)	837, 2056
Bpu10I	CCTNAGC	1 cut(s)	326
Bpu14I	TTCGAA	1 cut(s)	1438
BpuMI	CCSGG	2 cut(s)	832, 1991
BsaAI	YACGTR	2 cut(s)	1013, 1298
BsaBI	GATNNNNATC	1 cut(s)	925
BsaHI	GRCGYC	1 cut(s)	408
BsaI	GGTCTC	1 cut(s)	2057
BsaJI	CCNNGG	7 cut(s)	570, 618, 624, 630, 1053, 1858, 1959
BsaWI	WCCGGW	2 cut(s)	769, 1267
BsaXI	ACNNNNNCTCC	6 cut(s)	314, 344, 810, 840, 864, 894
Bsc4I	CCNNNNNNNGG	6 cut(s)	248, 747, 1298, 1630, 1693, 1993
Bse118I	RCCGGY	3 cut(s)	374, 759, 1291
Bse1I	ACTGG	1 cut(s)	820
Bse21I	CCTNAGG	1 cut(s)	1913
Bse3DI	GCAATG	4 cut(s)	162, 1101, 1745, 1795
Bse8I	GATNNNNATC	1 cut(s)	925
BseBI	CCWGG	1 cut(s)	632
BseDI	CCNNGG	7 cut(s)	570, 618, 624, 630, 1053, 1858, 1959
BseGI	GGATG	1 cut(s)	1983
BseJI	GATNNNNATC	1 cut(s)	925
BseLI	CCNNNNNNNGG	6 cut(s)	248, 747, 1298, 1630, 1693, 1993
BseMI	GCAATG	4 cut(s)	162, 1101, 1745, 1795
BseMII	CTCAG	3 cut(s)	317, 476, 566
BseNI	ACTGG	1 cut(s)	820
BseSI	GKGCMC	2 cut(s)	254, 767
BseXI	GCAGC	2 cut(s)	565, 568
BseYI	CCCAGC	2 cut(s)	1064, 1592
Bsh1236I	CGCG	1 cut(s)	1569
BsiHKAI	GWGCWC	9 cut(s)	321, 332, 537, 767, 1143, 1170, 1371, 1821, 1838
BsiHKCI	CYCGRG	2 cut(s)	1076, 1889
BslFI	GGGAC	1 cut(s)	1093
BslI	CCNNNNNNNGG	6 cut(s)	248, 747, 1298, 1630, 1693, 1993
BsmAI	GTCTC	3 cut(s)	557, 1892, 2057
BsmFI	GGGAC	1 cut(s)	1093
BsmI	GAATGC	1 cut(s)	1598
Bso31I	GGTCTC	1 cut(s)	2057
BsoBI	CYCGRG	2 cut(s)	1076, 1889
Bsp119I	TTCGAA	1 cut(s)	1438
Bsp143I	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
Bsp19I	CCATGG	1 cut(s)	570
BspCNI	CTCAG	3 cut(s)	318, 475, 565
BspFNI	CGCG	1 cut(s)	1569
BspHI	TCATGA	4 cut(s)	717, 837, 1143, 1762
BspLI	GGNNCC	1 cut(s)	686
BspMI	ACCTGC	2 cut(s)	626, 745
BspPI	GGATC	2 cut(s)	614, 724
BspQI	GCTCTTC	1 cut(s)	1507
BspT104I	TTCGAA	1 cut(s)	1438
BspTNI	GGTCTC	1 cut(s)	2057
BsrDI	GCAATG	4 cut(s)	162, 1101, 1745, 1795
BsrFI	RCCGGY	3 cut(s)	374, 759, 1291
BsrI	ACTGG	1 cut(s)	820
BssAI	RCCGGY	3 cut(s)	374, 759, 1291
BssECI	CCNNGG	7 cut(s)	570, 618, 624, 630, 1053, 1858, 1959
BssMI	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
BssNI	GRCGYC	1 cut(s)	408
BssT1I	CCWWGG	2 cut(s)	570, 1959
Bst2UI	CCWGG	1 cut(s)	632
Bst4CI	ACNGT	6 cut(s)	543, 644, 909, 1322, 1633, 1859
Bst6I	CTCTTC	4 cut(s)	1472, 1507, 1692, 1697
BstACI	GRCGYC	1 cut(s)	408
BstAPI	GCANNNNNTGC	1 cut(s)	1201
BstBAI	YACGTR	2 cut(s)	1013, 1298
BstBI	TTCGAA	1 cut(s)	1438
BstC8I	GCNNGC	7 cut(s)	376, 516, 520, 615, 757, 1060, 1567
BstDEI	CTNAG	6 cut(s)	313, 326, 462, 526, 552, 1913
BstDSI	CCRYGG	5 cut(s)	570, 618, 624, 1053, 1858
BstF5I	GGATG	1 cut(s)	1983
BstFNI	CGCG	1 cut(s)	1569
BstH2I	RGCGCY	1 cut(s)	1501
BstHHI	GCGC	3 cut(s)	1064, 1500, 1831
BstKTI	GATC	8 cut(s)	105, 609, 732, 1336, 1351, 1719, 1762, 1951
BstMAI	GTCTC	3 cut(s)	557, 1892, 2057
BstMBI	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
BstNI	CCWGG	1 cut(s)	632
BstNSI	RCATGY	2 cut(s)	1266, 1386
BstSCI	CCNGG	3 cut(s)	630, 830, 1989
BstSFI	CTRYAG	1 cut(s)	1609
BstSLI	GKGCMC	2 cut(s)	254, 767
BstUI	CGCG	1 cut(s)	1569
BstV1I	GCAGC	2 cut(s)	565, 568
BstV2I	GAAGAC	5 cut(s)	278, 412, 466, 874, 945
BstX2I	RGATCY	1 cut(s)	606
BstYI	RGATCY	1 cut(s)	606
Bsu36I	CCTNAGG	1 cut(s)	1913
BtgI	CCRYGG	5 cut(s)	570, 618, 624, 1053, 1858
BtgZI	GCGATG	1 cut(s)	1727
BtsCI	GGATG	1 cut(s)	1983
BtsI	GCAGTG	1 cut(s)	1851
BtsIMutI	CAGTG	2 cut(s)	276, 1851
BveI	ACCTGC	2 cut(s)	626, 745
Cac8I	GCNNGC	7 cut(s)	376, 516, 520, 615, 757, 1060, 1567
CciI	TCATGA	4 cut(s)	717, 837, 1143, 1762
CfoI	GCGC	3 cut(s)	1064, 1500, 1831
Cfr10I	RCCGGY	3 cut(s)	374, 759, 1291
CpoI	CGGWCCG	1 cut(s)	1633
CseI	GACGC	2 cut(s)	397, 1138
CspI	CGGWCCG	1 cut(s)	1633
DdeI	CTNAG	6 cut(s)	313, 326, 462, 526, 552, 1913
DpnI	GATC	8 cut(s)	104, 608, 731, 1335, 1350, 1718, 1761, 1950
DpnII	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
DraI	TTTAAA	1 cut(s)	63
DriI	GACNNNNNGTC	1 cut(s)	946
EaeI	YGGCCR	5 cut(s)	615, 621, 652, 828, 1278
Eam1104I	CTCTTC	4 cut(s)	1472, 1507, 1692, 1697
Eam1105I	GACNNNNNGTC	1 cut(s)	946
EarI	CTCTTC	4 cut(s)	1472, 1507, 1692, 1697
EciI	GGCGGA	1 cut(s)	1709
Ecl136II	GAGCTC	5 cut(s)	319, 330, 535, 1168, 1369
Eco130I	CCWWGG	2 cut(s)	570, 1959
Eco147I	AGGCCT	1 cut(s)	1958
Eco24I	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
Eco31I	GGTCTC	1 cut(s)	2057
Eco47I	GGWCC	6 cut(s)	194, 685, 1394, 1519, 1633, 1855
Eco47III	AGCGCT	1 cut(s)	1499
Eco53kI	GAGCTC	5 cut(s)	319, 330, 535, 1168, 1369
Eco57I	CTGAAG	1 cut(s)	183
Eco72I	CACGTG	1 cut(s)	1298
Eco81I	CCTNAGG	1 cut(s)	1913
Eco88I	CYCGRG	2 cut(s)	1076, 1889
EcoICRI	GAGCTC	5 cut(s)	319, 330, 535, 1168, 1369
EcoO109I	RGGNCCY	1 cut(s)	685
EcoRI	GAATTC	3 cut(s)	183, 855, 1374
EcoRII	CCWGG	1 cut(s)	630
EcoT14I	CCWWGG	2 cut(s)	570, 1959
EcoT22I	ATGCAT	1 cut(s)	680
EcoT38I	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
ErhI	CCWWGG	2 cut(s)	570, 1959
FaqI	GGGAC	1 cut(s)	1093
FauI	CCCGC	1 cut(s)	527
FbaI	TGATCA	3 cut(s)	102, 1348, 1759
Fnu4HI	GCNGC	8 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710
FokI	GGATG	1 cut(s)	1990
FriOI	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
Fsp4HI	GCNGC	8 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710
FspBI	CTAG	1 cut(s)	1229
GlaI	GCGC	3 cut(s)	1063, 1499, 1830
GluI	GCNGC	8 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710
GsaI	CCCAGC	2 cut(s)	1068, 1596
GsuI	CTGGAG	2 cut(s)	837, 2056
HaeII	RGCGCY	1 cut(s)	1501
HgaI	GACGC	2 cut(s)	397, 1138
HhaI	GCGC	3 cut(s)	1064, 1500, 1831
Hin1I	GRCGYC	1 cut(s)	408
Hin6I	GCGC	3 cut(s)	1062, 1498, 1829
HinP1I	GCGC	3 cut(s)	1062, 1498, 1829
HincII	GTYRAC	3 cut(s)	546, 589, 1807
HindII	GTYRAC	3 cut(s)	546, 589, 1807
HindIII	AAGCTT	1 cut(s)	1420
HinfI	GANTC	8 cut(s)	266, 888, 1072, 1108, 1121, 1214, 1302, 1430
HpaI	GTTAAC	1 cut(s)	546
HphI	GGTGA	6 cut(s)	16, 490, 650, 1310, 1583, 1766
Hpy166II	GTNNAC	8 cut(s)	459, 546, 589, 765, 1325, 1665, 1807, 1855
Hpy188I	TCNGA	9 cut(s)	23, 93, 209, 555, 734, 887, 900, 1373, 1977
Hpy8I	GTNNAC	8 cut(s)	459, 546, 589, 765, 1325, 1665, 1807, 1855
Hpy99I	CGWCG	4 cut(s)	113, 413, 596, 2024
HpyCH4III	ACNGT	6 cut(s)	543, 644, 909, 1322, 1633, 1859
HpyCH4IV	ACGT	4 cut(s)	594, 1012, 1082, 1297
HpyF3I	CTNAG	6 cut(s)	313, 326, 462, 526, 552, 1913
HpySE526I	ACGT	4 cut(s)	594, 1012, 1082, 1297
Hsp92I	GRCGYC	1 cut(s)	408
HspAI	GCGC	3 cut(s)	1062, 1498, 1829
KroI	GCCGGC	1 cut(s)	374
KroNI	GCCGGC	1 cut(s)	376
Ksp22I	TGATCA	3 cut(s)	102, 1348, 1759
KspAI	GTTAAC	1 cut(s)	546
Kzo9I	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
LguI	GCTCTTC	1 cut(s)	1507
Lsp1109I	GCAGC	2 cut(s)	565, 568
LweI	GCATC	4 cut(s)	38, 411, 531, 986
MaeI	CTAG	1 cut(s)	1229
MaeII	ACGT	4 cut(s)	594, 1012, 1082, 1297
MaeIII	GTNAC	2 cut(s)	638, 1627
MalI	GATC	8 cut(s)	104, 608, 731, 1335, 1350, 1718, 1761, 1950
MboI	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
MfeI	CAATTG	2 cut(s)	432, 1616
MflI	RGATCY	1 cut(s)	606
MlsI	TGGCCA	2 cut(s)	654, 1280
MluNI	TGGCCA	2 cut(s)	654, 1280
MlyI	GAGTC	3 cut(s)	882, 1130, 1223
MmeI	TCCRAC	4 cut(s)	418, 757, 1546, 1955
Mox20I	TGGCCA	2 cut(s)	654, 1280
Mph1103I	ATGCAT	1 cut(s)	680
MroNI	GCCGGC	1 cut(s)	374
MroXI	GAANNNNTTC	1 cut(s)	859
MscI	TGGCCA	2 cut(s)	654, 1280
MseI	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2068
Msp20I	TGGCCA	2 cut(s)	654, 1280
MspA1I	CMGCKG	1 cut(s)	1346
MspR9I	CCNGG	3 cut(s)	632, 832, 1991
MunI	CAATTG	2 cut(s)	432, 1616
Mva1269I	GAATGC	1 cut(s)	1598
MvaI	CCWGG	1 cut(s)	632
MvnI	CGCG	1 cut(s)	1569
NaeI	GCCGGC	1 cut(s)	376
NciI	CCSGG	2 cut(s)	832, 1991
NcoI	CCATGG	1 cut(s)	570
NdeII	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
NgoMIV	GCCGGC	1 cut(s)	374
NlaIV	GGNNCC	1 cut(s)	686
NmuCI	GTSAC	2 cut(s)	638, 1627
NsiI	ATGCAT	1 cut(s)	680
NspI	RCATGY	2 cut(s)	1266, 1386
NspV	TTCGAA	1 cut(s)	1438
PaeR7I	CTCGAG	1 cut(s)	1889
PagI	TCATGA	4 cut(s)	717, 837, 1143, 1762
PaqCI	CACCTGC	1 cut(s)	626
PceI	AGGCCT	1 cut(s)	1958
PciI	ACATGT	1 cut(s)	1262
PciSI	GCTCTTC	1 cut(s)	1507
PcsI	WCGNNNNNNNCGW	2 cut(s)	405, 1318
PctI	GAATGC	1 cut(s)	1598
PdiI	GCCGGC	1 cut(s)	376
PdmI	GAANNNNTTC	1 cut(s)	859
PfeI	GAWTC	5 cut(s)	266, 1072, 1108, 1302, 1430
PflMI	CCANNNNNTGG	1 cut(s)	747
PkrI	GCNGC	8 cut(s)	119, 577, 580, 583, 1019, 1357, 1693, 1711
PleI	GAGTC	3 cut(s)	882, 1129, 1222
PmaCI	CACGTG	1 cut(s)	1298
PmlI	CACGTG	1 cut(s)	1298
PpsI	GAGTC	3 cut(s)	882, 1129, 1222
Ppu21I	YACGTR	2 cut(s)	1013, 1298
PpuMI	RGGWCCY	1 cut(s)	685
PscI	ACATGT	1 cut(s)	1262
PshBI	ATTAAT	1 cut(s)	923
PsiI	TTATAA	1 cut(s)	1049
Psp124BI	GAGCTC	5 cut(s)	321, 332, 537, 1170, 1371
Psp5II	RGGWCCY	1 cut(s)	685
Psp6I	CCWGG	1 cut(s)	630
PspCI	CACGTG	1 cut(s)	1298
PspFI	CCCAGC	2 cut(s)	1064, 1592
PspGI	CCWGG	1 cut(s)	630
PspN4I	GGNNCC	1 cut(s)	686
PspPPI	RGGWCCY	1 cut(s)	685
PsuI	RGATCY	1 cut(s)	606
PvuII	CAGCTG	1 cut(s)	1346
Rsr2I	CGGWCCG	1 cut(s)	1633
RsrII	CGGWCCG	1 cut(s)	1633
SacI	GAGCTC	5 cut(s)	321, 332, 537, 1170, 1371
SapI	GCTCTTC	1 cut(s)	1507
SaqAI	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2068
SatI	GCNGC	8 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710
Sau3AI	GATC	8 cut(s)	102, 606, 729, 1333, 1348, 1716, 1759, 1948
SchI	GAGTC	3 cut(s)	882, 1130, 1223
ScrFI	CCNGG	3 cut(s)	632, 832, 1991
SfaNI	GCATC	4 cut(s)	38, 411, 531, 986
SfcI	CTRYAG	1 cut(s)	1609
Sfr274I	CTCGAG	1 cut(s)	1889
SfuI	TTCGAA	1 cut(s)	1438
SinI	GGWCC	6 cut(s)	194, 685, 1394, 1519, 1633, 1855
SlaI	CTCGAG	1 cut(s)	1889
SmlI	CTYRAG	1 cut(s)	1889
SmoI	CTYRAG	1 cut(s)	1889
SseBI	AGGCCT	1 cut(s)	1958
SspI	AATATT	1 cut(s)	261
SspMI	CTAG	1 cut(s)	1229
SstI	GAGCTC	5 cut(s)	321, 332, 537, 1170, 1371
StuI	AGGCCT	1 cut(s)	1958
StyD4I	CCNGG	3 cut(s)	630, 830, 1989
StyI	CCWWGG	2 cut(s)	570, 1959
TaaI	ACNGT	6 cut(s)	543, 644, 909, 1322, 1633, 1859
TaiI	ACGT	4 cut(s)	597, 1015, 1085, 1300
TaqI	TCGA	8 cut(s)	321, 929, 1438, 1531, 1558, 1890, 1951, 2022
TauI	GCSGC	6 cut(s)	120, 578, 1020, 1358, 1694, 1712
TfiI	GAWTC	5 cut(s)	266, 1072, 1108, 1302, 1430
Tru1I	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2068
Tru9I	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2068
TscAI	CASTG	2 cut(s)	276, 1851
TseFI	GTSAC	2 cut(s)	638, 1627
TseI	GCWGC	2 cut(s)	578, 581
Tsp45I	GTSAC	2 cut(s)	638, 1627
TspDTI	ATGAA	8 cut(s)	466, 706, 758, 826, 848, 1160, 1246, 1379
TspGWI	ACGGA	2 cut(s)	413, 1508
TspRI	CASTG	2 cut(s)	276, 1851
Van91I	CCANNNNNTGG	1 cut(s)	747
VneI	GTGCAC	1 cut(s)	763
VpaK11BI	GGWCC	6 cut(s)	194, 685, 1394, 1519, 1633, 1855
VspI	ATTAAT	1 cut(s)	923
XapI	RAATTY	3 cut(s)	183, 855, 1374
XceI	RCATGY	2 cut(s)	1266, 1386
XhoI	CTCGAG	1 cut(s)	1889
XmnI	GAANNNNTTC	1 cut(s)	859
XspI	CTAG	1 cut(s)	1229
Zsp2I	ATGCAT	1 cut(s)	680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.